A Draft Genome of the Striped Catfish, Pangasianodon Hypophthalmus, for Comparative Analysis of Genes Relevant to Development An
Kim et al. BMC Genomics (2018) 19:733 https://doi.org/10.1186/s12864-018-5079-x RESEARCHARTICLE Open Access A draft genome of the striped catfish, Pangasianodon hypophthalmus, for comparative analysis of genes relevant to development and a resource for aquaculture improvement Oanh T. P. Kim1*† , Phuong T. Nguyen1†, Eiichi Shoguchi2†, Kanako Hisata2, Thuy T. B. Vo1, Jun Inoue2, Chuya Shinzato2,4, Binh T. N. Le1, Koki Nishitsuji2, Miyuki Kanda3, Vu H. Nguyen1, Hai V. Nong1 and Noriyuki Satoh2* Abstract Background: The striped catfish, Pangasianodon hypophthalmus, is a freshwater and benthopelagic fish common in the Mekong River delta. Catfish constitute a valuable source of dietary protein. Therefore, they are cultured worldwide, and P. hypophthalmus is a food staple in the Mekong area. However, genetic information about the culture stock, is unavailable for breeding improvement, although genetics of the channel catfish, Ictalurus punctatus, has been reported. Toacquiregenomesequencedataasausefulresourcefor marker-assisted breeding, we decoded a draft genome of P. hypophthalmus and performed comparative analyses. Results: Using the Illumina platform, we obtained both nuclear and mitochondrial DNA sequences. Molecular phylogeny using the mitochondrial genome confirmed that P. hypophthalmus is a member of the family Pangasiidae and is nested within a clade including the families Cranoglanididae and Ictaluridae. The nuclear genome was estimated at approximately 700 Mb, assembled into 568 scaffolds with an N50 of 14.29 Mbp, and was estimated to contain ~ 28,600 protein-coding genes, comparable to those of channel catfish and zebrafish. Interestingly, zebrafish produce gadusol, but genes for biosynthesis of this sunscreen compound have been lost from catfish genomes.
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