ARTICLE 155 et al. 673U  5 Phthirusa Phthirusa et al !""4 2  $     `SQ* ! Key words: >    Pucciniomycotina   Urediniomycetes IMA · 6(1): 155–162 (2015) 155–162 · 6(1): FUNGUS IMA    54    ` G<  .5 ! et al.566!V*@Z   [     <>/?  Uromyces      Loranthaceae !""#      ?  V      "7 G 7  _6"7 G !   47 G !    #5 G  !   #5 G 4 [ $   ;G      O !z      sequencing V    %           $        /           %        .   !4*      M56 GV    O  ?        [     .     <>/ $        GV/+          <I<!""6;G              54%[64ZV ?<>/; ; 653U  )*     4*!6‹  !4UUG `@U.#66!WU     X       U  G   Z %   +   Z+         ;           [$ !6       .        54/!!6     %   K  $ !6\ 3* <>/K .        %;  NV@7.   NV@4.                ;  NV@   >/    5 % * F/ 566FX  I9  !""6   * 6    ! U % <  +;  ! ,  , Loranthaceae U U U. , as it Phthirusa urbanianus       + O  G       U. urbanianus U U. nilagiricus Uromyces    A. goyazense ,   ‰   Q@  Aecidum goyazense    + O   ! Loranthaceae loranthi U U. socius  U. loranthi, %         V    Aecidium goyazense          , U. bahiensis stelis

 UG % !  + O    phthirusae U. evastigatus, U , Phthirusa et al. 5664                  , V$  

            [                          Uromyces           <  W   N  G  +   Z %   +  #6"!6."66+  / *@Z    <>/ %      V             Article info:@  [5_U 56!4K/ [_`56!4K;  ["`56!4 © 2015 International Mycological Association © 2015 International Mycological conditions: distribute and transmit the work, under the following are free to share - to copy, You Attribution: ) from the Brazilian Cerrado the Brazilian ) from (Loranthaceae stelis !   @G@O Aecidium goyazense , on Aecidium for nov. nom. 789686 Non-commercial:No derivative works:Any of the above conditions can be waived if you get For any reuse or distribution, you must make clear to others the license terms of this work, which can be found at http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode.                         Nothing in this license impairs or restricts the author’s moral rights. permission from the copyright holder. * % MATERIALS & METHODS MATERIALS +    <   W  V      %         \              %          /        !4M563      U   O    @?                $        >               *   /?  %      [    Souza et al.

Table 1.]+     Uromyces hawksworthii          Species GenBank accession numbers (LSU)* Source and Country Uromyces hawksworthiiZ+55_E5 2 E5!!_" ;   + O  Uromyces hawksworthiiZ+55E#4 2 E5!!76 ;   + O  Cumminsiella mirabilissima <ƒ_474_! / 566F]   ARTICLE Cumminsiella mirabilissima /W75F56F U  et al566_]   Puccinia coronata <ƒ_4745F / 566FZ@/ Puccinia coronata SZE4!!7!   et al.56!! Puccinia graminis /W455!## + et al.!""5 Puccinia graminis 9ƒ7!5F7E <  et al.56!! Puccinia hemerocallidis ]Z64E656 < $et al56!6Z@/ Puccinia hemerocallidis <ƒ_474!" / 566FZ@/ Puccinia heucherae <ƒ_4"#6! 9  et al.566#Z2 Puccinia heucherae <ƒ_4"#65 9  et al.566#Z2 Puccinia hordei <ƒ_4745# / 566FZ@/ Puccinia melanocephala 2;56!E_E ‰ et al.Z 56!7G  Puccinia melanocephala 2;56!E_" ‰ et alZ 56!7G  Puccinia nakanishikii ]Z64E665 < $et al.56!6Z@/ Puccinia peperomiae SZE4!!7F   et al.56!!G Uromyces acuminatus ]Z!6"5E5 et al.56!6S  Uromyces appendiculatus 2U57"E#6 UV  56!7/  Uromyces appendiculatus /#74#67 U  et al.Z 5664 Uromyces ari-triphylli <ƒ_4745" / 566FZ@/ Uromyces ixiae <ƒ"!##_E U  et al.566#@ /  Uromyces ixiae <ƒ"!##_" U  et al.566#@ /  Uromyces pisi /W75F56! U  et al.566_G S  Uromyces striatus 9ƒ7!5F45 <  et al.56!!Q  Uromyces striatus 9ƒ_!#4!5 * et al.56!4 G   Uromyces trifoli ]Z"_FF_7   et al.56!!G Uromyces viciae-fabae 2`#!F_7_ ;  IU2O 56!7>   Uromyces viciae-fabae /W75F!"" U  et al.566_G S  Uromyces vignae /+!!4F7" Get al.5667`   Melampsora larici-populina    `ƒ675546 +  et al.56!5Z@/ Ê*@Z[ <>/  

         !‹V/S    Pucciniaceae+ et al.!""5U  et al.566_G %   O ZX      [  O  et al.5667/ 566FU et al.566F9  et al.  ;G       S$@/;.NV&Z@+ 566#U  et al.566#et al.56!6< $et al.56!6 ?  / +  /+N_!_6$U <  et al56!!  et al.56!!+ et al. 56!5    G  Z %  +   UV  56!7;   U2O 56!7* et al. V   ?       + S  56!4 /      ?       9 56!5/?     W/@V/           ?             [     UZ@G*S&S 5667    US]/          ? > ? %F  V  et al.56!!/         ]+   [\\  %\    Œ     ]   \V !           V            V +/@S  [\\   \    !#FF# +     +N    PHYLOGENETIC ANALYSIS    U % G  U  G         *@Z?+     G?     ]+ R +N                U  +*/@V +    +*/@V  U UQ

156 IMA FUNGUS 789686 on Pihthirusa (Loranthaceae)

V   . %     %    Diagnosis[ / Uromyces    Phthirusa stelis ARTICLE     %            Loranthaceae                       $     ]V ŽNŽ]  %            !5        V                  .            GN; S@     U   et al. 56!6   U +  % _5  ?   I 9  Type[Brazil: ] @ ; Phthirusa stelis 56!5W UGUG       † Loranthus~/!E"5Ule 1909 +5"74M K     !6666666 V   +  ] N/ G  ;   % !666      !6666  +  !4 7E)75!5P@&7#:4E)554_P‰ % V[  5466        .   Phthirusa stelis"W 56!7J. C. DianeseZ+UG     ;            55E#4Mepitype designated hereU+V56!4_4  Œ .         #466  V     Melampsora larici-populina Description[ Spermogonia    Aecidia 4MF    %   O W V    566" $  & _66M766 )                                 \                      %     64M!    RESULTS        !5        K peridial cells _6M36M4# & 5!M22M_4 ) Phylogeny                      / [   ?    *@Z  NV@ <>/  Aecidiospores 57M54M5"M_4 & !#M5!M54M5#4               )          %           Z+55_E5    Z+55E#4   %        K walls 5M_4M74 ) V  [   5E@ <>/ NV@ %  Uredinia Telia!M2M_      ? !466 746   %/               >  *@Z[Z+55_E5„2 E5!!_"Z+55E#4„2 E5!!76 %        \       NV@[ Z+55_E5„2 E5!!_# Z+55E#4„2 E5!!_E V               *@Z  NV@ ?           .        Teliospores _7M       V     <>/ _"M7_M7F & !EM 55M57 M5F4 ) !.  . *@Z       [     K                               @SU         %K lateral wall 54M_M7 3    %  [        4MFM#3  .  Kpedicels   9et al.56!7VNV@?  7EME7M!7_M!4#&7M4MFM#3      ]+  Z>NVS> et al.56!7+              .            *@Z    $      \$           9et al 56!7 _! $              Other specimens examined  %Phthirusa stelis[Brazil[  *@Z %  V     +  ] N/ G  ; +   !6_#       "#         !EU 56!7J. C. DianeseZ+U55E#"K/ > G    %5!! %  E!6  % Z %   +   Z %      !#@56!5 E. S. C. de Souza Z+ U 55_E"K ; ? Q

VOLUME 6 · NO. 1 157 Souza et al. ARTICLE

Fig. 1. A–H. Uromyces hawksworthiiZ+UG55E#4[    %      A. S      B. N      C.V         D. G     %     E. /          F.;       $  G. /  H.< %      + [/MG„5<„ 463S„_663W„563]„!639„!3

158 IMA FUNGUS 789686 on Pihthirusa (Loranthaceae) ARTICLE

Fig. 2. A–E. Uromyces hawksworthiiZ+UG55E#4[A.G            $  B.S     C. /       %   .  D.V  @SUE.V             @SU+ [/„!+„5G„!63<„!663S„43

Key to Uromyces species on Loranthaceae

! V   . _6M74&5!M_63    E3   U. nilagiricus  V    .  5

5! V     763 3  V      763 #

_5 V            U. ornatipes  V     % 7

7_ V      .      4  V      % F

47 V         U. circumscriptus  V   %53   U. bahiensis

F7 V    [% K                     U. loranthi  V     K             U. socius

#5 V   .    463 E  V  .      "6 !F63!6

VOLUME 6 · NO. 1 159 Souza et al. ARTICLE

Fig. 3. ;        +         *@Z ?  Uromyces    $ V +         %  %6#4      ]+         V           + ?      <>/?           %V      Melampsora larici-populina

E# V   % [                 K       %      U. phthiruzae  V     %      %  %  "

"E V  % . K    %  U. urbanianus  V           %  U. evastigatus

!6# V     .% K     .   U. struthanthi  V     .% K    %  U. hawksworthii

DISCUSSION    ]+  +           ]+          *@Z Uromyces hawksworthii            ? Puccinia heucherae 9@45"F\64]+           + O   Loranthaceae, in <ƒ_4"#65KN "Ez„!6_F\!6F6U. acuminatus                        GV.X6E6F5_._ ]+  ]Z!6"5E5K N  "E z  _4      .[   %    „ !6_4\!64" U. ari-triphylli ZF_# ]+  <ƒ_4745"K       W    U. hawksworthii N  "E z „ !6_7\!64# Puccinia graminis Z.F#7            $     ]+ 9ƒ7!5F7EKN "Ez„!65_\!67E 

160 IMA FUNGUS 789686 on Pihthirusa (Loranthaceae)

P. hordei/WVQ*.N<!765]+ <ƒ_4745#KN  /  `G!"!EUredinales]       ARTICLE "Ez„!6!#\!67_/           ‰‰<9 MNNAecidiaceae$ %Puccinia      Uromyces hawksworthii  $       American Journal of Botany 5[756M77F                 Uromyces +  V< X    +  @U ]O O < 9   <@ et al. ari-triphylli Puccinia peperomiaeW _‰     !""5 S%           [                  „ 6"E      >/?Molecular Phylogenetics      U. hawksworthii        and Evolution 1[5_!M57!        $  /       @  +  ;S /  UG >  ] 56!5 W     /         O      Populus angustifolia        +   <>/ ?                  > /  Fungal Biology 116[#"5ME6!        G‰9V  VQ2    U5667U    V                  Uromyces appendiculatus U. Aecidium goyazense9 !E"4       vignae `  Mycoscience 45[5__M577       %    V   Uromyces <  U*/@  /U/U ?  UW GG `G56!6         Œ / 4_! ;         Mycological G?    %  %        Progress 9[74"M7FE Uromyces hawksworthii  <``<`*!""6N   <>/    V  [     Uromyces    Focus 12[!_M!4 Loranthaceae  %  9et al.5664; . S  G5667UZ@G*S[  ?      @O I ;   56!7 N          $ BMC Bioinformatics 5[!!_                9 V56!5+ S %#6"[+   ?                     ‰ "4\"E\52\‹;\#             ; .@O 9`WW  U+G % ` //9;]5664 I ;   56!7 %         G        Uredinales+ O     @SU   Uromyces  N  ;?  `  +   `    Loranthaceae     $    U. nilagiricus, `  + O     Loranthus  N    9 ;!E"4W  OHedwigia 34[EEM!!5         %   V       9  +/< ]/* G+566#W    Puccinia     *  /          heucheraeHeuchera  Z2Plant Pathology 56[_45         I     !"46 92<>  9@/ //    /9+ Set al. 56!7 +              Uromyces Q [            Loranthaceae      .[  [N56!7Fungal Diversity 67[5!M!54  Q  [ %     2 + 9 < ‰;]W  2UV@X!""#/      U. euphlebius, U. ornatipes, U.     R       loranthi, U. phthirusae  Usocius@!"56/    Puccinia  G   Phytopathology 87[4F4M4#! !"!4!"!E; .@OI;  56!7V * UUG SG  `VG `‰ @2et al.56!4           %    <     [          U. bahiensis, U. circumscriptus, Uevastigatus, U V ?U &  . ;G Canadian Journal of Plant Pathology loranthi, Uphthirusae, U. struthanthi Uurnabianus 37["5M!64 N           U. loranthi     U  ‰+ <‰ UQ    W566_;       %   U phthirusae    [                  %      <>/?Canadian Journal of Botany 81[!5M5_ U  ‰‰ [+<U U‰ [U`566#;              Puccinia  ACKNOWLEDGEMENTS Uromyces. Mycological Research 111[!#FM!E4 U ;+] `/  ;2 V2/9  <@566F ‰  U O @ O   >]U   < U    %     Wallemiomycetes[     V    G>;?\+ O  [      Œ   BasidiomycotaCanadian Journal of     ;;+NQ.G ; Œ  Botany 84[!#"7M!E64 UV  /  ] /<‰  @ %  ] 56!7 Uredinopsis pteridis Desmella aneimiae [     Pucciniales REFERENCES   Pteridophyta /  Australasian Plant Disease Notes 9[!7" / UG566FV  %   .%       U  U/;  ‰@ OV56!6G   GN; S@@   UredinalesMycoscience 47[!!5M!55 ]         Proceedings /   `G !"!4 >    Uredineae N‹ Bulletin of Torrey of the Gateway Computing Environments Workshop (GCE)!7 Botanical Club 42[4E4M4"_ >%56!6>Q  */[!ME

VOLUME 6 · NO. 1 161 Souza et al.

U %`U‰ 999 @!""4;        / 566" FigTree X   !5_ S   [ N    in Ganoderma              S%  + Z %  S    54@  <>/?Mycologia 87[55_M5_E ?  WVU%  U ;/ <*<  /et al >  992<;  `   UV *et al. 56!5 U  _5[ [   +       56!7 N %  NV@ ?      [                  Systematic

ARTICLE    Fungal Diversity 67[!!M!" Biology 61[4_"M475 ;  UU2O S9G56!7/    @9;!"56>%   Annales Mycologici 18[ >     Myosotidium     !47M!F6 G  N Phytotaxa 174[55_M5_6 V   2 ;   < ;   > @   ] >  U 2  @ ; .@OQ;  U56!7@ Uromyces 56!!U 4[ %        Puccinilaes, Basidiomycota  Loranthaceae Tropical Plant  $     %        $  Pathology 39[!7!M!4_    Molecular Biology and Evolution 28[5#_!M ;  U @  @  +/ 566! @? %   5#_"         \ .       X    9   U !""6      [   Uromyces pisi     Euphorbia cyparissias Mycological   O    [  <>/ %  Research 105[4#MFF Cryptococcus Journal of Bacteriology 172[75_EM757F ;  <+V5667U   %   9U  /U< $*`G    */56!6W      [ %  /        Uromyces acuminatusHonckenya peploides    +      %        Systematic      Plant Disease 94[5#" Biology 53[#"_ME6E    G +  ; U  U 56!! W    \       V@     2 !"46/      Uredinales O         U  XNNNProceedings of the Indian Academy of Science,+  /<>  5E@Revista de Biología Tropical 3[!65M!!6 59[4!#M476

162 IMA FUNGUS