Objective Plant Pathology
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Sugarcane Wilt: New Insights Into Pathogen Identity, Variability and Pathogenicity
® Functional Plant Science and Biotechnology ©2012 Global Science Books Sugarcane Wilt: New Insights into Pathogen Identity, Variability and Pathogenicity Rasappa Viswanathan* • Muthusamy Poongothai • Palaniyandi Malathi • Amalraj R. Sundar Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore 641007, India Corresponding author : * [email protected] ABSTRACT Wilt of sugarcane, a fungal disease is known to cause significant damage to sugarcane production and productivity in India and other countries for the past one century. Although sugarcane wilt is known in India for a long time, research work on this important disease is totally lacking. The causal organism was found to vary with time and investigator and could not reproduce the disease under artificial conditions in the field. We have made detailed disease surveys in 13 major sugarcane growing states in the country and 263 Fusarium isolates were isolated. We have established the variation in Fusarium isolates associated with sugarcane wilt, based on cultural, morphological, pathogenic and molecular characterization of 117 isolates. Critical observations in the conventional techniques combined with molecular biological approaches clearly established that Fusarium sacchari as the causal agent of the disease. Other Fusarium sp isolated from wilt infected sugarcane stalks were found to be either secondary invaders or non-pathogenic in nature. We have developed an artificial simulation technique to induce wilt in sugarcane under field conditions and -
PCBR 1956.Pdf (858.1Kb)
UNITED STATES DEPARTMENT OF AGRICULTURE Forest Service Region One BR REPORTS Annual - 1956 WHITE PINE BLISTER RUST CONTROL Calendar Year 1956 INF. IV. National Park Program I. Highlights of the 1956 Season The 1956 objectives of the National Park Service Region II white pine blister rust control program were accomplished. The program was planned and conducted as in previous years according to the cooperative arrangements between the Na- tional Park Service and the U. S. Forest Service. National Park Service personnel participating: Glacier Elmer Fladniark, chief ranger *A. D. Cannavina, s~pervisory park ranger Paul Webb, district ranger Yellowstone Otto Brown, chief ranger •~H. o. Edwards, assistant chief ranger Rocky Mountain: Harry During, chief ranger ->~Merle Stitt, staff ranger Grand Teton '-'"Ernest K. Field, chief r~ger Maynard Barrows, National Park Service consulting forester U. s .. forest Service· representatives: ~John C. Gynn, forester C. M. Chapman, forester The Na tionai Park Service Director approves new areas for -control. In January 1956, John c. Gynn met with National Park Service Region II Director Howard w. Baker, Regional Forester Frank ff. Childs, Fore:ster Maynard Barrows, and other members of their .s-taff at Omaha, "Nebraska, to review the results of the 1955 white pine and ribes survey on l7,270 acres of National Park lands. The group deter- mined ·the following areas should be incl\Jded in the pro;gram and the areas were later approved by the Director of the National Park Service. ~15- Glacier - expanded protection zones to present control areas only. Unit Acres Park Headquarters 300 East Glacier (Rising Sun Campground) 370 Twd Medicine 200 Total 870 Yellowstone New Unit Antelope Creek 1,390 Canyon 11,470 Fishing Bridge 2,090 Craig Pass (extension) 5,240 Total 20,190 Grand Teton New Unit Snake River (De adman 's Bar) 1,010 Grand Total 22,070 New areas surveyed at Roc~Mounta~~· At the request of Superintendent James V. -
US EPA, Pesticide Product Label, COPPER SULFATE
c C EPA Reg. Number: Date of U.S. ENVIRONMENTAL PROTECTION AGENCY Issuance: Office of Chemical Safety and Pollution Prevention Office of Pesticide Programs 88633-3 AUG 26 20H Registration Division (7504P) 1200 Pennsylvania Ave.', N.W. Washington, DC 20460 Term of Issuance: Conditional NOTICE OF PESTICIDE: X_ Registration Name of Pesticide Product: Reregistration Copper Sulfate [under FIFRA, as amended) Pentahydrate Name and Address of Registrant (include ZIP Code): Mailed to: Delta Agro Chemicals Connie Welch Road 6 No 15 A Agent of Delta Agro Chemicals Maddi, Cairo, Egypt toXcel Toxicology & Regulatory Affairs 7140 Heritage Village Plaza Gainesville, VA 20155 On the basis of information furnished by the registrant, the above named pesticide is hereby registered under the Federal Insecticide, Fungicide and Rodenticide Act Registration is in no way to be construed as an endorsement or recommendation of this product by the Agency. Jn order to protect health and the environment, the Administrator, on his motion, may at any time suspend or cancel the registration of a pesticide in accordance with the Act. The acceptance of any name in connection with the registration of a product under this Act is not to be construed as giving the registrant a right to exclusive use of the name or to its use if it has been covered by others. The basic formulation CSF dated April 9, 2014 of the product referred to above, submitted in connection with registration under the Federal Insecticide, Fungicide, and Rodenticide Act is acceptable. The basic CSF will be added to your file. This product is conditionally registered in accordance with FIFRA section 3(c)(7)(A) provided that you: Page 1 of 2 Signature of Approving Official: Date: AUG 2 6 20H Tony Kish, Product Manager1^) Fungicide Branch/Registration Division/OPP/OCSPP (7504P) EPA Form 8570-6 C~ C" Notice of Pesticide Registration— v_- ""y Copper Sulfate Pentahydrate " // EPA Reg. -
Monilochaetes and Allied Genera of the Glomerellales, and a Reconsideration of Families in the Microascales
available online at www.studiesinmycology.org StudieS in Mycology 68: 163–191. 2011. doi:10.3114/sim.2011.68.07 Monilochaetes and allied genera of the Glomerellales, and a reconsideration of families in the Microascales M. Réblová1*, W. Gams2 and K.A. Seifert3 1Department of Taxonomy, Institute of Botany of the Academy of Sciences, CZ – 252 43 Průhonice, Czech Republic; 2Molenweg 15, 3743CK Baarn, The Netherlands; 3Biodiversity (Mycology and Botany), Agriculture and Agri-Food Canada, Ottawa, Ontario, K1A 0C6, Canada *Correspondence: Martina Réblová, [email protected] Abstract: We examined the phylogenetic relationships of two species that mimic Chaetosphaeria in teleomorph and anamorph morphologies, Chaetosphaeria tulasneorum with a Cylindrotrichum anamorph and Australiasca queenslandica with a Dischloridium anamorph. Four data sets were analysed: a) the internal transcribed spacer region including ITS1, 5.8S rDNA and ITS2 (ITS), b) nc28S (ncLSU) rDNA, c) nc18S (ncSSU) rDNA, and d) a combined data set of ncLSU-ncSSU-RPB2 (ribosomal polymerase B2). The traditional placement of Ch. tulasneorum in the Microascales based on ncLSU sequences is unsupported and Australiasca does not belong to the Chaetosphaeriaceae. Both holomorph species are nested within the Glomerellales. A new genus, Reticulascus, is introduced for Ch. tulasneorum with associated Cylindrotrichum anamorph; another species of Reticulascus and its anamorph in Cylindrotrichum are described as new. The taxonomic structure of the Glomerellales is clarified and the name is validly published. As delimited here, it includes three families, the Glomerellaceae and the newly described Australiascaceae and Reticulascaceae. Based on ITS and ncLSU rDNA sequence analyses, we confirm the synonymy of the anamorph generaDischloridium with Monilochaetes. -
Plant-Parasitic Algae (Chlorophyta: Trentepohliales) in American Samoa1
Plant-Parasitic Algae (Chlorophyta: Trentepohliales) in American Samoa1 Fnd E. Erooks 2 Abstract: A survey conducted betweenJune 2000 and May 2002 on the island of Tutuila, American Samoa, recorded filamentous green algae of the order Tren tepohliales (CWorophyta) and their plant hosts. Putative pathogenicity of the parasitic genus Cephaleuros and its lichenized state, Strig;ula, was also inves tigated. Three genera and nine species were identified: Cephaleuros (five spp.), Phycopeltis (two spp.), and Stomatochroon (two spp.). A widely distributed species of Trentepohlia was not classified. These algae occurred on 146 plant species and cultivars in 101 genera and 48 families; 90% of the hosts were dicotyledonous plants. Cephaleuros spp. have aroused worldwide curiosity, confusion, and con cern for over a century. Their hyphaelike filaments, sporangiophores, and as sociated plant damage have led unsuspecting plant pathologists to misidentify them as fungi, and some phycologists question their parasitic ability. Of the five species of Cephaleuros identified, C. virescens was the most prevalent, followed by C. parasiticus. Leaf tissue beneath thalli of Cephaleuros spp. on 124 different hosts was dissected with a scalpel and depth of necrosis evaluated using a four point scale. No injury was observed beneath thalli on 6% of the hosts, but full thickness necrosis occurred on leaves of 43% of hosts. Tissue damage beneath nonlichenized Cephaleuros thalli was equal to or greater than damage beneath lichenized thalli (Strig;ula elegans). In spite of moderate to severe leaf necrosis caused by Cephaleuros spp., damage was usually confined to older leaves near the base of plants. Unhealthy, crowded, poorly maintained plants tended to have the highest percentage of leaf surface area affected by TrentepoWiales. -
Inoculation with Mycorrhizal Fungi and Irrigation Management Shape the Bacterial and Fungal Communities and Networks in Vineyard Soils
microorganisms Article Inoculation with Mycorrhizal Fungi and Irrigation Management Shape the Bacterial and Fungal Communities and Networks in Vineyard Soils Nazareth Torres † , Runze Yu and S. Kaan Kurtural * Department of Viticulture and Enology, University of California Davis, 1 Shields Avenue, Davis, CA 95616, USA; [email protected] (N.T.); [email protected] (R.Y.) * Correspondence: [email protected] † Current address: Advanced Fruit and Grape Growing Group, Public University of Navarra, 31006 Pamplona, Spain. Abstract: Vineyard-living microbiota affect grapevine health and adaptation to changing environ- ments and determine the biological quality of soils that strongly influence wine quality. However, their abundance and interactions may be affected by vineyard management. The present study was conducted to assess whether the vineyard soil microbiome was altered by the use of biostimulants (arbuscular mycorrhizal fungi (AMF) inoculation vs. non-inoculated) and/or irrigation management (fully irrigated vs. half irrigated). Bacterial and fungal communities in vineyard soils were shaped by both time course and soil management (i.e., the use of biostimulants and irrigation). Regarding alpha diversity, fungal communities were more responsive to treatments, whereas changes in beta diversity were mainly recorded in the bacterial communities. Edaphic factors rarely influence bacte- rial and fungal communities. Microbial network analyses suggested that the bacterial associations Citation: Torres, N.; Yu, R.; Kurtural, were weaker than the fungal ones under half irrigation and that the inoculation with AMF led to S.K. Inoculation with Mycorrhizal the increase in positive associations between vineyard-soil-living microbes. Altogether, the results Fungi and Irrigation Management highlight the need for more studies on the effect of management practices, especially the addition Shape the Bacterial and Fungal of AMF on cropping systems, to fully understand the factors that drive their variability, strengthen Communities and Networks in Vineyard Soils. -
Early Blight of Tomato
Dr. Yonghao Li Department of Plant Pathology and Ecology The Connecticut Agricultural Experiment Station 123 Huntington Street, P. O. Box 1106 New Haven, CT 06504 Phone: (203) 974-8601 Fax: (203) 974-8502 Founded in 1875 Email: [email protected] Putting science to work for society Website: www.ct.gov/caes EARLY BLIGHT OF TOMATO Early blight, caused by Alternaria solani, is a sunscald of fruit. common fungal disease of tomatoes grown in Stem infections can occur at any age, and fields, greenhouses, and high tunnels. In result in small, dark, slightly sunken areas that warm, rainy and wet weather, epidemics of enlarge to form circular or elongated this disease can cause severe defoliation, yield concentric lesions (Figure 2). loss, and poor fruit quality. The fungus also infects potato. Fruit can be infected during the green or ripe SYMPTOMS AND DIAGNOSTICS The fungus can infect most parts of a tomato plant, including leaves, stems, and fruit. Lesions on leaves first appear as small (less than 1/16 inch) brown spots surrounded by yellow discolorations. Diagnostic symptoms develop as the spots enlarge and become dark brown or black lesions with concentric rings, usually 1/3 to 1/4 inch in diameter (Figure 1). Under favorable conditions, many lesions Figure 2. An elongated concentric lesion coalesce and result in severe defoliation and (arrow) on the stem. Figure 3. A black and sunken lesion on the tomato fruit. Figure 1. Dark brown or black concentric Figure 3. A black and sunken lesion (arrow) lesions (arrows) on tomato leaves. on the tomato fruit. -
Neoproterozoic Origin and Multiple Transitions to Macroscopic Growth in Green Seaweeds
Neoproterozoic origin and multiple transitions to macroscopic growth in green seaweeds Andrea Del Cortonaa,b,c,d,1, Christopher J. Jacksone, François Bucchinib,c, Michiel Van Belb,c, Sofie D’hondta, f g h i,j,k e Pavel Skaloud , Charles F. Delwiche , Andrew H. Knoll , John A. Raven , Heroen Verbruggen , Klaas Vandepoeleb,c,d,1,2, Olivier De Clercka,1,2, and Frederik Leliaerta,l,1,2 aDepartment of Biology, Phycology Research Group, Ghent University, 9000 Ghent, Belgium; bDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Zwijnaarde, Belgium; cVlaams Instituut voor Biotechnologie Center for Plant Systems Biology, 9052 Zwijnaarde, Belgium; dBioinformatics Institute Ghent, Ghent University, 9052 Zwijnaarde, Belgium; eSchool of Biosciences, University of Melbourne, Melbourne, VIC 3010, Australia; fDepartment of Botany, Faculty of Science, Charles University, CZ-12800 Prague 2, Czech Republic; gDepartment of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742; hDepartment of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138; iDivision of Plant Sciences, University of Dundee at the James Hutton Institute, Dundee DD2 5DA, United Kingdom; jSchool of Biological Sciences, University of Western Australia, WA 6009, Australia; kClimate Change Cluster, University of Technology, Ultimo, NSW 2006, Australia; and lMeise Botanic Garden, 1860 Meise, Belgium Edited by Pamela S. Soltis, University of Florida, Gainesville, FL, and approved December 13, 2019 (received for review June 11, 2019) The Neoproterozoic Era records the transition from a largely clear interpretation of how many times and when green seaweeds bacterial to a predominantly eukaryotic phototrophic world, creat- emerged from unicellular ancestors (8). ing the foundation for the complex benthic ecosystems that have There is general consensus that an early split in the evolution sustained Metazoa from the Ediacaran Period onward. -
11 the Evolutionary Strategy of Claviceps
Pažoutová S. (2002) Evolutionary strategy of Claviceps. In: Clavicipitalean Fungi: Evolutionary Biology, Chemistry, Biocontrol and Cultural Impacts. White JF, Bacon CW, Hywel-Jones NL (Eds.) Marcel Dekker, New York, Basel, pp.329-354. 11 The Evolutionary Strategy of Claviceps Sylvie Pažoutová Institute of Microbiology, Czech Academy of Sciences Vídeòská 1083, 142 20 Prague, Czech Republic 1. INTRODUCTION Members of the genus Claviceps are specialized parasites of grasses, rushes and sedges that specifically infect florets. The host reproductive organs are replaced with a sclerotium. However, it has been shown that after artificial inoculation, C. purpurea can grow and form sclerotia on stem meristems (Lewis, 1956) so that there is a capacity for epiphytic and endophytic growth. C. phalaridis, an Australian endemite, colonizes whole plants of pooid hosts in a way similar to Epichloë and it forms sclerotia in all florets of the infected plant, rendering it sterile (Walker, 1957; 1970). Until now, about 45 teleomorph species of Claviceps have been described, but presumably many species may exist only in anamorphic (sphacelial) stage and therefore go unnoticed. Although C. purpurea is type species for the genus, it is in many aspects untypical, because most Claviceps species originate from tropical regions, colonize panicoid grasses, produce macroconidia and microconidia in their sphacelial stage and are able of microcyclic conidiation from macroconidia. Species on panicoid hosts with monogeneric to polygeneric host ranges predominate. 329 2. PHYLOGENETIC TREE We compared sequences of ITS1-5.8S-ITS2 rDNA region for 19 species of Claviceps, Database sequences of Myrothecium atroviride (AJ302002) (outgroup from Bionectriaceae), Epichloe amarillans (L07141), Atkinsonella hypoxylon (U57405) and Myriogenospora atramentosa (U57407) were included to root the tree among other related genera. -
Blister Blight Disease of Tea: an Enigma Chayanika Chaliha and Eeshan Kalita
Chapter Blister Blight Disease of Tea: An Enigma Chayanika Chaliha and Eeshan Kalita Abstract Tea is one of the most popular beverages consumed across the world and is also considered a major cash crop in countries with a moderately hot and humid climate. Tea is produced from the leaves of woody, perennial, and monoculture crop tea plants. The tea leaves being the source of production the foliar diseases which may be caused by a variety of bacteria, fungi, and other pests have serious impacts on production. The blis- ter blight disease is one such serious foliar tea disease caused by the obligate biotrophic fungus Exobasidium vexans. E. vexans, belonging to the phylum basidiomycete primarily infects the young succulent harvestable tea leaves and results in ~40% yield crop loss. It reportedly alters the critical biochemical characteristics of tea such as catechin, flavo- noid, phenol, as well as the aroma in severely affected plants. The disease is managed, so far, by administering high doses of copper-based chemical fungicides. Although alternate approaches such as the use of biocontrol agents, biotic and abiotic elicitors for inducing systemic acquired resistance, and transgenic resistant varieties have been tested, they are far from being adopted worldwide. As the research on blister blight disease is chiefly focussed towards the evaluation of defense responses in tea plants, during infection very little is yet known about the pathogenesis and the factors contrib- uting to the disease. The purpose of this chapter is to explore blister blight disease and to highlight the current challenges involved in understanding the pathogen and patho- genic mechanism that could significantly contribute to better disease management. -
Potato Di~Ea~E~ Early Blight Phillip Wharton and William Kirk Department of Plant Pathology, Michigan State University Early Blight Symptoms Alternaria Solani (E
Extension Bulletin E-2991 • New • May 2007 ~----------------------- -----------------------~------~ MICHI6AN Potato Di~ea~e~ Early Blight Phillip Wharton and William Kirk Department of Plant Pathology, Michigan State University Early Blight Symptoms Alternaria solani (E. & M.) Jones and Grout Foliar symptoms of early blight first appear as small, (Hyphomycetes, Hyphales) irregular to circular dark brown spots on the lower (older) leaves. These spots may range in size from Introduction a pinpoint to 1/8 inch in diameter (Fig. 1). As the Early blight is a very common disease of potato that spots enlarge, they become restricted by leaf veins is found in most potato-growing areas. Although it and take on an angular shape. Early in the growing occurs annually to some degree in most production season, lesions on young, fully expanded, succulent areas, the timing of its appearance and the rate of leaves may be larger - up to 112 inch in diameter disease progress help determine the impact on the - and may, because of their size, be confused with potato crop. The disease occurs over a wide range of late blight lesions (Fig. 2). Leaf lesions are relatively climatic conditions and depends in large part on the easy to identify in the field because lesion develop frequency of foliage wetting from rainfall, fog, dew or ment is characterized by a series of dark concentric irrigation, on the nutritional status of foliage and on rings alternating with bands of light tan tissue (Fig. 3). cultivar susceptibility. Though losses rarely exceed 20 A narrow band of chlorotic tissue often surrounds percent, if left uncontrolled, the disease can be very each lesion, and extensive chlorosis of infected destructive. -
EPPO Standards
, EUROPEAN AND MEDITERRANEAN PLANT PROTECTION ORGANIZATION ЕВРОПЕЙСКАЯ И СРЕДИЗЕМНОМОРСКАЯ ОРГАНИЗАЦИЯ ПО КАРАНТИНУ И ЗАЩИТЕ РАСТЕНИЙ ORGANIZATION EUROPEENNE ET MEDITERRANEENNE POUR LA PROTECTION DES PLANTES 05-11646 PPM point 8.202/9267 PEST RISK ASSESSMENT SCHEME Organism: Claviceps africana Assessor(s): Riccardo Bugiani Plant Protection Service – Regione Emilia-Romagna (Italy) Date: February 2005 Approximate time spent on the assessment 2 PEST RISK ASSESSMENT STAGE 1: INITIATION Reasons for PRA During the second part of nineties, Claviceps africana, responsible for sorghum ergot, spread from the original area and new outbreaks in Mis en forme America and Australia were found. This fact caused a general concern and warning at the global level. The disease was added to EPPO Alert List. Considering that sorghum is an important crop in Emilia-Romagna region (Italy) a PRA has been conducted in order to evaluate the phytosanitary risk posed in the pathogen. Identify pest This section examines the identity of the pest to ensure that the assessment is being performed on a real identifiable organism and that the biological and other information used in the assessment is relevant to the organism in question. 1. Is the organism clearly a single taxonomic entity and can it be YES Taxonomy is based on data of Ainsworth and Bisbi's adequately distinguished from other entities of the same rank? (http://www.indexfungorum.org/Names/fundic.asp) if yes go to 3 Phylum: Ascomyceta if no go to 2 Class: Ascomycetes Subclass: Sordiaromycetidae Order: Hypocreales Family: Clavicipitaceae Genus: Claviceps Species: africana Claviceps africana was recognised as a distinct species in 1991 after the first description of its teleomorph by Frederickson, Mante & and de Milliano.