Master Any Type of Liquid Eppendorf Solutions

Water Viscous Dense Volatile Infectious / radioactive Detergent Foaming Mechanical pipettes Electronical pipettes e.g. glycerol, oil e.g. sulfuric acid, e.g. acetone, ethanol e.g. biohazard material e.g. Tween 20, e.g. protein-containing caesium chloride Triton™ X-100 liquids Type of Liquid Type

> Air-cushion pipettes are > High resistance to flow > Influence on size of > Air-cushion expands > Aerosols contaminate > Reduced surface tension > Foam is created Advantages Advantages optimized to the physical > Liquid residues stay air-cushion > Liquid drips out of the tip pipette > Liquid residues stick to > Liquid residues remain > Easy to clean > High reproducibility properties of water attached to inside tip wall > Dispensed volume too > Imprecise results > Threat to human health the inner wall of the tip in the tip > Economical > Ergonomic working > Imprecise results low or too high and sample safety > Imprecise results > Imprecise results > Lightweight > Multifunctionality Potential Potential problems Observations

> Optimally suitable for > Work slowly > Adjust pipette to > Prewet at least 5 times > Use filter tips > Use tips with low > Reverse pipetting > Eppendorf Research® plus > Eppendorf Xplorer® the use of water > Reverse pipetting liquid density > Reverse pipetting > Automated systems retention effect > Eppendorf Reference® 2 > Eppendorf Xplorer® plus > No adaptation necessary > Adjust liquid type*1 > Adjust liquid type*1 > Adjust liquid type*1 protect user and sample > Adjust liquid type*1 > Pipet Helper® > Easypet® 3 > epMotion® pipettes Prevention Air-cushion

> Serial pipetting for > Higher precision > Higher precision > Higher precision > Higher precision > Higher precision > Higher precision > Multipette® M4 > Multipette® E3/E3x multiple samples regardless of physical regardless of physical regardless of physical regardless of physical regardless of physical regardless of physical properties of liquid properties of liquid properties of liquid properties of liquid properties of liquid properties of liquid > Serial pipetting > Serial pipetting > Serial pipetting > Serial pipetting > Serial pipetting > Serial pipetting Positive Positive dispenser displacement

> Varitip S*3,4 system > Varitip P*2 allows > Varitip P*2 allows > Varitip P*2 allows > Varitip P*2 allows > Varitip P*2 allows > Varitip P*2 allows > Varipette® 4720 allows accurate pipetting accurate pipetting, for accurate pipetting, for accurate pipetting, accurate pipetting, for accurate pipetting, for accurate pipetting, for from large bottles and example from beakers example from beakers for example from beakers example from beakers example from beakers example from beakers narrow vessels > Varitip S system and valve for drip-free dispensing Positive Positive pipettes displacement *2 *3 *4 Recommendations

> Liquid dispensing directly > Liquid dispensing directly > Liquid dispensing directly > Liquid dispensing directly > Liquid dispensing directly > Liquid dispensing directly > Liquid dispensing directly > Varispenser® 2/2x > Eppendorf Top Buret™ from supply bottles from supply bottles up to a from supply bottles up to a from supply bottles up to from supply bottles from supply bottles up to a from supply bottles viscosity of 500 mm2/s density of 2.2 g/cm3 a vapor pressure of viscosity of 500 mm2/s 500 mbar Positive Positive bottletop dispenser

*1 This option is only available on automated systems and electric pipettes *2, 3, 4 See Varipette® 4720 for corresponding Eppendorf Varitips®