DOCSLIB.ORG

Species Relationships of Lycoris Endemic to Korea Evaluated by RAPD and Snps of Nrdna-ITS Regions

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Species Relationships of Lycoris Endemic to Korea Evaluated by RAPD and Snps of Nrdna-ITS Regions Hort. Environ. Biotechnol. 52(2):145-151. 2011. DOI 10.1007/s13580-011-0100-1 Research Report Species Relationships of Lycoris Endemic to Korea Evaluated by RAPD and SNPs of nrDNA-ITS Regions Yong Kweon Yoo1, Tao Yuan2, Jong Suk Lee3, Ae Kyung Lee4, Mark S. Roh5, Siro Kurita6, and Jeung Keun Suh4* 1Division of Life Science, Mokpo National University, Muan 534-729, Korea 2College of Landscape Architecture, Beijing Forestry University, Beijing, PR China 3Department of Horticulture, Chungnam National University, Daejeon 305-764, Korea 4School of Bio-Resources Science, Dankook University, Cheonan 330-714, Korea 5US Department of Agriculture, Agricultural Research Service, US National Arboretum, Floral and Nursery Plants Research Unit, Beltsville, MD 20705, USA 6Laboratory of Phylogenetic Botany, Chiba University, Chiba 263-8522, Japan *Corresponding author: [email protected] Received May 17, 2010 / Accepted December 20, 2010 GKorean Society for Horticultural Science and Springer 2011 Abstract. This study was performed to investigate the species relationships and variation of Lycoris Herb. (Amaryllidaceae) species using random amplification of polymorphic DNA (RAPD) markers. Also, single nucleotide polymorphisms (SNPs) of internal transcribed spacer 1, 5.8S ribosomal RNA gene and internal transcribed spacer 2 regions in Lycoris sanguinea var. koreana were analyzed. All accessions formed 6 major clusters; cluster A with all L. sanguinea and L. chejuensis; cluster B with 3 accessions of L. flavescens; cluster C with 8 accessions of F. flavescens var. flavescens; cluster D with 10 accessions of L. uydoensis; cluster E with L. chinensis var. sinuolata and 4 accessions of L. uydoensis; and cluster F with all L. radiata. Five haplotypes were observed; L. sanguinea and L. chejuensis having the haplotype 1 with bases of CTTATATATAT; L. chinensis var. sinuolata and all L. flavescens. Lycoris incarnata and L. aurea, non-endemic to Korea had haplotype 2 and 5, respectively. Genetic variations in L. flavescens, L. chinensis var. sinuolata, and L. uydoensis are revealed based on the analysis of molecular variances (AMOVA) and haploid types analyzed by sequence analysis. It is suggested that L. chejuensis may result from hybridization involving L. sanguinea var. koreana due to a close affinity between L. sanguinea complex and L. chejuensis. Nomenclature for L. chejuensis and L. flavescens whether they should be described as a hybrid origin should be discussed in the future. Additional key words: genetic variance, hybrid origin, internal transcribed spacer region, molecular markers, random amplification of polymorphic DNA, single nucleotide polymorphisms =bhfcXiWh]cb Based on the morphological characters, L. chinensis var. sinuolata, L. sanguinea var. koreana, L. flavescens, L. The genus Lycoris consists of about 20 species distributed uydoensis, and L. radiata var. radiata were divided into two in warm temperate zones mainly in China, Korea, and Japan (Tae and Ko, 1995b) or four groups (Tae and Ko, 1996), (Hsu et al., 1994). Lycoris chejuensis K. H. Tae & S. C. Ko and based on RAPD analysis into two or three (Tae and Ko, (L. × chejuensis S. Kurita & P.S. Hsu), L. chinensis Traub. 1997a, 1997b) or one group (Tae et al., 2008), and these var. sinuolata K. Tae et S. Ko ex K. Tae et S. Ko, L. flaves- groupings differed greatly among studies. Lycoris flavescens cens M. Kim & S. Lee, L. sanguinea var. koreana (Nakai) and L. chejuensis, for example, belonged to the same group T. Koyama (L. koreana Nakai), and L. uydoensis M. Kim, in all studies, which is different from the previous study L. radiata (L’Hér.) Herb. var. radiata, and L. squamigera (Roh et al., 2002). Maxim. have been reported endemic to Korea (Kim, 2004; Difficulty to identify Lycoris species based on the mor- Kim and Lee, 1991; Kurita and Hsu, 1998; Lee and Kim, phology could be solved by molecular markers generated 1987; Tae and Ko, 1993, 1995a, 2003; Tae et al., 1987). from randomly amplified polymorphic DNA (RAPD) (Roh 146 Yong Kweon Yoo, Tao Yuan, Jong Suk Lee, Ae Kyung Lee, Mark S. Roh, Siro Kurita, and Jeung Keun Suh et al., 2002). Using isozyme, genetic variance was reported AUhYf]U`g UbX AYh\cXg only in L. flavescens (Lee et al., 2001). Describing the new species of hybrid was difficult due to a phenotypic inter- D`Ubh AUhYf]U`g mediacy (Gottlieb, 1972; Rieseberg, 1997), morphological Lycoris species from the south-western part of Korea characters can not be used effectively for germplasm with a were collected as indicated (Table 1 and Fig. 1). Over 7 very minor difference. The genetic variations were revealed years, they were verified by floral morphology (Fig. 5 and in Lycoris species using inter simple sequence repeat (ISSR) Roh et al., 2002). Total genomic DNA was isolated from 50 analysis (Shi et al., 2006) and RAPD (Roh et al., 2002). mg dried as described (Roh et al., 2006) and quantified by Single nucleotide polymorphisms (SNPs) (Brooks, 1999) of NanoDrop D-1000 Spectrophotometer (Thermo Fisher Scie- internal transcribed spacer regions and the 5.8S ribosomal ntific Waltham, MA, USA). DNA region (nrDNA-ITS region) have been useful for identification at inter-specific levels in Corylopsis (Roh et F5D8 UbX 8UhU 5bU`mg]g al., 2007) and Lycoris (Shi et al., 1998). Six RAPD primers (A9, A11, B13, B17, and B18; Operon Extensive studies on the relationships among Lycoris Technologies, Alameda, CA) were selected (Roh et al., species native to Korea and their genetic diversities using 2002) and polymerase chain reaction (PCR) was performed molecular markers had not been performed except L. flave- and the amplified products were separated on an agarose gel scens using the isozyme markers. Therefore, this study was and stained as described previously (Joung and Roh, 2004; carried out to investigate the relationships among species and Roh et al., 2007). Polymorphic RAPD bands ranging from the genetic diversities of Lycoris species collected from 400 bp to 1,500 bp were scored and the corresponding matrix diverse geographic locations using molecular markers gene- was used to construct a dendrogram using the interior-bran- rated by RAPD and SNPs of nrDNA-ITS regions. ching (IB) test applied to the neighbour-joining (NE) by 6 Fig. 1. Overall collection sites of Lycoris native to Korea. Sites (1; Is. Uy, 2; Te. Naeso, 3; Te. Sunwoon, 4; Te. Bagyang/Mt. Naejang, 5; Mt. Chuwol, 6; Mt. Boolgap, 7; Mt. Gyeryong, 8; Fig. 2. Dendrogram constructed by the neighbor-joining analyzed Sancheon, 9; Daecheon, 10; Anduck valley and Odeung, and by interior branching test generated from RAPD markers. 11; Eoreum. The collection sites for germplasm in China and Bootstrap values > 70% following 1,000 replications are indicated Japan are not indicated. next to the relevant nodes. Hort. Environ. Biotechnol. 52(2):145-151. 2011. 147 Molecular Evolutionary Genetics Analysis (MEGA, version radiata var. radiata (48, 56, 58, 60-62), L. uydoensis (A: 4.0; Tamura et al., 2007). 33-37; B: 63-71, 82), and L. chejuensis (A: 73-81; B: 72). Based on the RAPD dendrogram (Fig. 2), accessions Data was then analyzed for analysis of the molecular variance were grouped into six species and 7 sub-populations among (AMOVA) and Nei’s genetic distance was calculated by the species; L. chinensis var. sinuolata (A; 1-8, 15, 16; B: Nei’s unbiased measures of genetic distance (Nei, 1978) 9-14; C: 17, 30; D: 29), L. sanguinea var. koreana (18 - 28, using Arlequin ver. 3.1 (Excoffier et al., 2005). 31, 32), L. flavescens (A:49-55, 57, 59; B: 83-85) , L. Table 1. List of the Lycoris accessions with collection location and the source of germplasm. Accession numberz Scientific name Remarksy 1, 3, 5, 6, 7, 13, 14 L. chinensis var. sinuolata Mt. Boolgap, Yeongkwang, Cheonnam-Province, valley 1. 5 - light flower colorx 8, 9, 10, 11 L. chinensis var. sinuolata Mt. Boolgap, Yeongkwang, Cheonnam-Province, valley 2 15, 16 L. chinensis var. sinuolata Mt. Boolgap, Yeongkwang, Cheonnam-Province, valley 3 17 L. chinensis var. sinuolata Te. Bagyang, Changsung-Kun, Cheonnam-Province, temple garden 18 L. chinensis var. sinuolata Mt. Sunwoon, Kochang-Kun, Cheonbuk-Province. S. B. Park 19 L. chinensis var. sinuolata Mt. Sunwoon, Kochang-Kun, Cheonbuk-Province. hill 1 21, 22 L. sanguinea var. koreana Te. Bagyang, Changsung-Kun, Cheonnam-Province, valley 1 23, 24 L. sanguinea var. koreana Te. Bagyang, Changsung-Kun, Cheonnam-Province, valley 2 20, 25, 26, 27, 28 L. sanguinea var. koreana Te. Bagyang, Changsung-Kun, Cheonnam-Province, hill 2 (accession no. 20), valley 3 (accession no. 25 �G 28) 29, 30, 33, 34 L. sanguinea var.koreana Mt. Chuwol, Damyang-Kun, Cheonnam-Province, valley 1. 30 - light flower color 31, 32 L. chinensis var. sinuolata Mt. Chuwol, Damyang-Kun, Cheonnam-Province, valley 1. 31 -light flower color; 32 - light flower color 41 L. uydoensis Mt. Gyeryong, Gongju-Shi, Chungnam-Province, Mr. Choi’s resident, transplanted 42 L. uydoensis Chido-Ri, Is. Uy Cheonbuk-Province 43, 44, 45 L. uydoensis Yougol valley, Is. Uy Mr. Choi’s residence 46 L. sanguinea var. sanguinea Eikou Ooyabu and Mr. Oono, Japan 47 L. sanguinea var. kiusiana Eikou Ooyabu and Mr. Oono, Japan 48 L. chinensis var. chinensis Z. Zhao, Beijing Agricultural University, Beijing, China 49 L. incarnata Anduck valley, Joongmoon, Jeju, Korea 53 L. sanguinea var. koreana Jeju, Korea, white flower, collected as L. chejuensis 55 L. sanguinea var. sanguinea S. Kurita, Chiba University, Chiba, Japan 56 L. sanguinea var. kiusiana S. Kurita, Chiba University, Chiba, Japan. Collected from Nagasaki, Japan 57 L. longituba var. longituba S. Kurita, Chiba University, Chiba, Japan 58 L. × albiflora S. Kurita, Chiba University, Chiba, Japan. Collected from Kagoshima, Japan 59 L. radiata var. pumila S. Kurita, Chiba University, J Chiba, Japan. Collected from China 60 L. radiata var.
Load more

Recommended publications
  • Current Status of Protected Horticulture in Korea Ⅰ Protected Horticulture Research Institute
    2017-09-29 FFTC Workshop CONTENTs 2017. 9. 12. Current Status of Protected Horticulture in Korea Ⅰ Protected Horticulture Research Institute II Introduction III Greenhouse structure IV Energy saving technology V Environment control VI Smart farm VII Inho Yu Conclusion Protected Horticulture Research Institute National Institute of Horticultural & Herbal Science, RDA 1-42 Protected Horticulture Research Institute, NIHHS, RDA Protected Horticulture Research Institute, NIHHS, RDA Organization - 4 specialized laboratories: Greenhouse structure & materials, Greenhouse Energy saving, Hydroponic culture, Greenhouse ICT -16 researchers, 5 technician, and 50 research assistant members Seoul Suwon History - Founded in 1953 as Central Institute of Horticulture Technique - Changed in 1962 as Branch Station of Horticulture Research Station, RDA - Changed in 2015 as Protected Horticulture Research Institute, NIHHS, RDA Main Missions 1) Research on development and use for greenhouse structures, equipment, 360km away from Seoul structural safety assessment system for greenhouse crops 2) Development of energy saving techniques for protected horticulture to cope with high fuel costs 3) Research on hydroponics and fertigation systems for greenhouse crops 4) Development of precise control techniques of the greenhouse microclimate and root zone environment for high quality horticultural crop production 2-42 3-42 Protected Horticulture Research Institute, NIHHS, RDA Introduction Greenhouse farming in Korea has developed very quickly since 1990s. ㆍGreenhouse
    [Show full text]
  • Green Korea 2003 Green Korea 2003 Towards the Harmonization of Humans and Nature
    Green Korea 2003 www.me.go.kr Green Korea 2003 Towards the harmonization of humans and nature As the eaves in silhouette whisper our traditional beauty, the imagery opens a view of modern Korea where the past meets the future in harmony with nature. A View of the Han River Published by International Affairs Office, Ministry of Environment Government Complex Gwacheon, Jungangdong 1, Gwacheon-si, Gyeonggi-do, 427-729, Republic of Korea Ministry of Environment Tel. (822) 504-9244 Fax. (822) 504-9206 Republic of Korea This brochure uses recycled paper. Contents Preface ......................................................................................................2 Special Reports Environmental Vision of the Participatory Government ............................4 Environmentally Friendly World Cup ....................................................... 6 UNEP 8th Special Session of the Governing Council in Korea ...............10 Major Environmental Policies Development and Promotion of Environmental Technology ....................12 The Environmental Industry .......................................................................16 Environmental Education .........................................................................20 Preservation of the Natural Environment ...............................................22 Natural Gas Bus for Clear and Clean Sky .................................................26 Water Quality Management .......................................................................30 Management of Drinking Water
    [Show full text]
  • Brunei Cambodia
    Volume II Section II - East Asia and Pacific Brunei FMS - Fiscal Year 2012 Department of State On-Going Training Course Title Qty Training Location Student's Unit US Unit - US Qty Total Cost NWC International Fellows 4 NATIONAL WAR COLLEGE Army NATIONAL WAR COLLEGE $131,318 Fiscal Year 2012 On-Going Program Totals 4 $131,318 Service Academies - Fiscal Year 2012 Department of Defense On-Going Training Course Title Qty Training Location Student's Unit US Unit - US Qty Total Cost United States Air Force Academy 2 USAFA Colorado Springs, CO N/A USAFA $0 Fiscal Year 2012 On-Going Program Totals 2 $0 Brunei On-Going Fiscal Year 2012 Totals 6 $131,318 Brunei Fiscal Year 2013 Planned Totals 0 $0 Brunei Total 6 $131,318 Cambodia CTFP - Fiscal Year 2012 Department of Defense On-Going Training Course Title Qty Training Location Student's Unit US Unit - US Qty Total Cost ASC12-2 - Advanced Security Cooperation Course 2 Honolulu, Hawaii, United States General Department of Defence Services APSS $0 ASC12-2 - Advanced Security Cooperation Course 2 Honolulu, Hawaii, United States Ministry of National Defense APSS $0 Fiscal Year 2012 On-Going Program Totals 4 $0 FMF - Fiscal Year 2012 Department of State On-Going Training Course Title Qty Training Location Student's Unit US Unit - US Qty Total Cost Office of Anti-Human Trafficking and Minor American Language Course GET and SET 4 DLIELC, LACKLAND AFB TX DLIELC, LACKLAND AFB TX $41,048 Protection Fiscal Year 2012 On-Going Program Totals 4 $41,048 FMS - Fiscal Year 2012 Department of State On-Going Training
    [Show full text]
  • Administrative City SEJONG
    Administrative City SEJONG December 2015 2장간지 1. Background and Objective 2. History 3. Development Plan 4. Current Status 5. New Growth Engine 4장간지 1 Background and Objective 1-1. Background and Objective Balance National Strengthen National Development Competitiveness Relocate Relocate National Research Provide Great Attract Major Ministries Institutes Living Condition Functions (13,000 public (3,600 (Edu., culture, (Science, research, servants) researchers) welfare) business) 1-2. History March 2005 Enacted Special Law for City Construction January 2006 Established NAACC July 2006 Basic Plan / November 2006 Development Plan July 2007 Groundbreaking Ceremony July 2012 Established Sejong Special Self-governing City December2014 Relocated Ministries December2015 Completed Phase 1 1장간지 2 Development Plan 2-1. Location and Area 구 분 세종시 행복도시 면 적 464.84㎢ 72.91㎢ (서울의 3/4) (서울의 1/8) 인구 205,668명 106,348명 (’15.10.현재) 2-2. Project Budget Currency = USD Government and public Land compensation Committed facility construction $ 13 billion Land formation and (57.2%) Inter-regional infrastructure transportation network building 2-3. Development by Phases Administrative City Phase 3 of 500,000 population by 2030 Year 2021~2030 Population 500,000 Phase 2 Year 2016~2020 Population 300,000 Phase 1 Year 2007~2015 Improve Living Condition Population 150,000 Attract Private Sectors Construct Infrastructure 2-4. Urban Form Ring shape allows decentralization and non-hierarchy World’s First Ring City 조치원 오송역 Six Major Functions Jochiwon Osong Jeongan정안ICIC High-tech 청원IC Industry Cheongwon IC Medical / Central Welfare Administration 공 주 Gongju University / Culture / Research International Local Administration Two Ring Roads - Daedeok Techno Valley Daejeon Public Transportation 3 Current Status 3-1.
    [Show full text]
  • Democratic People's Republic of Korea
    Operational Environment & Threat Analysis Volume 10, Issue 1 January - March 2019 Democratic People’s Republic of Korea APPROVED FOR PUBLIC RELEASE; DISTRIBUTION IS UNLIMITED OEE Red Diamond published by TRADOC G-2 Operational INSIDE THIS ISSUE Environment & Threat Analysis Directorate, Fort Leavenworth, KS Topic Inquiries: Democratic People’s Republic of Korea: Angela Williams (DAC), Branch Chief, Training & Support The Hermit Kingdom .............................................. 3 Jennifer Dunn (DAC), Branch Chief, Analysis & Production OE&TA Staff: North Korea Penny Mellies (DAC) Director, OE&TA Threat Actor Overview ......................................... 11 [email protected] 913-684-7920 MAJ Megan Williams MP LO Jangmadang: Development of a Black [email protected] 913-684-7944 Market-Driven Economy ...................................... 14 WO2 Rob Whalley UK LO [email protected] 913-684-7994 The Nature of The Kim Family Regime: Paula Devers (DAC) Intelligence Specialist The Guerrilla Dynasty and Gulag State .................. 18 [email protected] 913-684-7907 Laura Deatrick (CTR) Editor Challenges to Engaging North Korea’s [email protected] 913-684-7925 Keith French (CTR) Geospatial Analyst Population through Information Operations .......... 23 [email protected] 913-684-7953 North Korea’s Methods to Counter Angela Williams (DAC) Branch Chief, T&S Enemy Wet Gap Crossings .................................... 26 [email protected] 913-684-7929 John Dalbey (CTR) Military Analyst Summary of “Assessment to Collapse in [email protected] 913-684-7939 TM the DPRK: A NSI Pathways Report” ..................... 28 Jerry England (DAC) Intelligence Specialist [email protected] 913-684-7934 Previous North Korean Red Rick Garcia (CTR) Military Analyst Diamond articles ................................................
    [Show full text]
  • Evaluating Sejong Special Self-Governing City's Impact on Local Economic Growth And
    저작자표시-비영리-변경금지 2.0 대한민국 이용자는 아래의 조건을 따르는 경우에 한하여 자유롭게 l 이 저작물을 복제, 배포, 전송, 전시, 공연 및 방송할 수 있습니다. 다음과 같은 조건을 따라야 합니다: 저작자표시. 귀하는 원저작자를 표시하여야 합니다. 비영리. 귀하는 이 저작물을 영리 목적으로 이용할 수 없습니다. 변경금지. 귀하는 이 저작물을 개작, 변형 또는 가공할 수 없습니다. l 귀하는, 이 저작물의 재이용이나 배포의 경우, 이 저작물에 적용된 이용허락조건 을 명확하게 나타내어야 합니다. l 저작권자로부터 별도의 허가를 받으면 이러한 조건들은 적용되지 않습니다. 저작권법에 따른 이용자의 권리는 위의 내용에 의하여 영향을 받지 않습니다. 이것은 이용허락규약(Legal Code)을 이해하기 쉽게 요약한 것입니다. Disclaimer 경제학 석사학위 논문 Evaluating Sejong Special Self-governing City’s Impact on Local Economic Growth and Standard of Living Using the Synthetic Control Method (SCM) 세종특별자치시가 지역경제발전과 삶의 질 개선에 미치는 영향의 SCM 분석 2020 년 2 월 서울대학교 대학원 경제학부 김 채 민 Evaluating Sejong Special Self-governing City’s Impact on Local Economic Growth and Standard of Living Using the Synthetic Control Method (SCM) 지도교수 김 소 영 이 논문을 경제학 석사학위 논문으로 제출함 2019 년 10 월 서울대학교 대학원 경제학부 김 채 민 김채민의 경제학석사학위 논문을 인준함 2020 년 1 월 위 원 장 이 철 인 (인) 부위원장 김 소 영 (인) 위 원 홍 재 화 (인) Evaluating Sejong Special Self-governing City’s Impact on Local Economic Growth and Standard of Living Using the Synthetic Control Method (SCM) Chae Min Kim Economics Department The Graduate School, Seoul National University Abstract This paper explores the efficacy of the South Korean government’s balanced national development plan, which entails establishing innovation cities in provincial areas to promote balanced growth by dispersing the capital’s infrastructure as well as its population.
    [Show full text]
  • Park Chan-Kyong and Sean Snyder by Doryun Chong
    EXHIBITION CHecKLIST 2002 Landschaft (Entfernung)/Landscape (Distance), Parallel World, Galerie K & S, Berlin Württembergischer Kunstverein, Stuttgart BRINKMANSHIP Park Chan-Kyong Korean Air France, La Vitrine & Glass Box, Paris Crisis Zones: World Cinema Now, Royal Ontario Museum, Institute for Contemporary Culture, Toronto Flying, 2005 P_A_U_S_E, 4th Gwangju Biennale, Gwangju, Korea video, color and sound, 13 min. Modelle für Morgen: Köln, European Kunsthalle, Cologne Courtesy the artist 2001 This Place is my Place: Begehrte Orte (Desired Spaces), Sunshine, Arts Council Korea, Insa Art Space, Seoul Kunstverein, Hamburg Power Passage, 2004/2010 2-channel video 2000 Door Slamming Festival, Mehringdamm 72, Berlin color and sound City Between 0 and 1, 1st Media City Seoul, Seoul Museum of Art, Seoul 2006 Courtesy the artist Liminal Spaces/grenzräume, Galerie für Zeitgenössische Kunst, PARK CHAN-KYONG Sindoan, 2008 Awards & Residencies Leipzig, Germany photographs, 23-5/8 x 35-5/8 in. each 2007 Faster! Bigger! Better!, ZKM, Center for Art and Media, Courtesy the artist Karlsruhe, Germany Tokyo Wonder Site Residency Program, Tokyo Sindoan, 2008 Everywhere, 5th Busan Biennale, Busan, Korea video, color and sound, 45 min. 2005 House for sale, Beyond Utrecht, Utrecht, Netherlands Courtesy the artist The Korean Culture and Arts Foundation Fever Variations, 6th Gwangju Biennale, Gwangju, Korea Three Cemeteries, 2009-10 2004 How to Do Things?, Künstraum Kreuzberg/Bethanien, Berlin 3 photographs and text, audio Hermès Korea Missulsang On Mobility II, de Appel, Amsterdam 32 x 53-1/2 in. each AND SEAN SNYDER 50 JPG, Centre de la Photographie, Geneva Commissioned by REDCAT, Los Angeles 2002 Periferic 7: Focussing Iasi, Bienala Internationala de Arta Akademie Schloss Solitude, Stuttgart Contemporana, Iasi, Romania Sean Snyder The Korean Culture and Arts Foundation 52.
    [Show full text]
  • Summary of Family Membership and Gender by Club MBR0018 As of February, 2009
    Summary of Family Membership and Gender by Club MBR0018 as of February, 2009 Club Fam. Unit Fam. Unit Club Ttl. Club Ttl. District Number Club Name HH's 1/2 Dues Females Male TOTAL District 355 D 25655 BUYEO 0 0 0 32 32 District 355 D 25663 DANGJIN 0 0 0 99 99 District 355 D 25665 DOONPO 0 0 0 83 83 District 355 D 25674 JOCHIWON 0 0 0 53 53 District 355 D 25680 KONGJU 0 0 0 34 34 District 355 D 25687 ONYANG 0 0 0 99 99 District 355 D 25723 TAECHON 0 0 0 30 30 District 355 D 25724 TAEJON 0 0 0 30 30 District 355 D 25725 TAEJON JOONGDO 0 0 0 34 34 District 355 D 25726 TAEJON HANBAD 0 0 0 48 48 District 355 D 25727 TAEJON CHOONGKYUNG 0 0 0 32 32 District 355 D 25728 TAEJON NEW TAEJON 0 0 0 42 42 District 355 D 25735 YESAN 0 0 0 52 52 District 355 D 29173 GEUM SAN 0 0 0 68 68 District 355 D 29174 HAP DUCK 0 0 0 37 37 District 355 D 29981 JANG HANG 0 0 0 33 33 District 355 D 30081 TAE AN LC 0 0 0 72 72 District 355 D 30137 SEOSAN 0 0 0 56 56 District 355 D 30486 TAEJON JOONGANG 0 0 0 24 24 District 355 D 30623 CHEONAN 0 0 0 56 56 District 355 D 30624 KANG KYONG 0 0 0 49 49 District 355 D 31504 NONSAN 0 0 0 43 43 District 355 D 32563 KUM NAM L C 0 0 0 68 68 District 355 D 32836 DOGO 0 0 0 14 14 District 355 D 33278 YUSEONG L C 0 0 0 33 33 District 355 D 33469 TAEJON BO MOON L C 0 0 0 39 39 District 355 D 33974 HONG SEONG L C 0 0 0 101 101 District 355 D 34419 SHINTANJIN L C 0 0 0 20 20 District 355 D 34567 TAEJON EAST 0 0 0 16 16 District 355 D 34703 BYEONGCHEON L C 0 0 0 52 52 District 355 D 34893 SHINCHANG L C 0 0 0 30 30 District 355 D 34894
    [Show full text]
  • Korean Red List of Threatened Species Korean Red List Second Edition of Threatened Species Second Edition Korean Red List of Threatened Species Second Edition
    Korean Red List Government Publications Registration Number : 11-1480592-000718-01 of Threatened Species Korean Red List of Threatened Species Korean Red List Second Edition of Threatened Species Second Edition Korean Red List of Threatened Species Second Edition 2014 NIBR National Institute of Biological Resources Publisher : National Institute of Biological Resources Editor in President : Sang-Bae Kim Edited by : Min-Hwan Suh, Byoung-Yoon Lee, Seung Tae Kim, Chan-Ho Park, Hyun-Kyoung Oh, Hee-Young Kim, Joon-Ho Lee, Sue Yeon Lee Copyright @ National Institute of Biological Resources, 2014. All rights reserved, First published August 2014 Printed by Jisungsa Government Publications Registration Number : 11-1480592-000718-01 ISBN Number : 9788968111037 93400 Korean Red List of Threatened Species Second Edition 2014 Regional Red List Committee in Korea Co-chair of the Committee Dr. Suh, Young Bae, Seoul National University Dr. Kim, Yong Jin, National Institute of Biological Resources Members of the Committee Dr. Bae, Yeon Jae, Korea University Dr. Bang, In-Chul, Soonchunhyang University Dr. Chae, Byung Soo, National Park Research Institute Dr. Cho, Sam-Rae, Kongju National University Dr. Cho, Young Bok, National History Museum of Hannam University Dr. Choi, Kee-Ryong, University of Ulsan Dr. Choi, Kwang Sik, Jeju National University Dr. Choi, Sei-Woong, Mokpo National University Dr. Choi, Young Gun, Yeongwol Cave Eco-Museum Ms. Chung, Sun Hwa, Ministry of Environment Dr. Hahn, Sang-Hun, National Institute of Biological Resourses Dr. Han, Ho-Yeon, Yonsei University Dr. Kim, Hyung Seop, Gangneung-Wonju National University Dr. Kim, Jong-Bum, Korea-PacificAmphibians-Reptiles Institute Dr. Kim, Seung-Tae, Seoul National University Dr.
    [Show full text]
  • Smart Energy Transition: an Evaluation of Cities in South Korea
    informatics Article Smart Energy Transition: An Evaluation of Cities in South Korea Yirang Lim 1,*, Jurian Edelenbos 2 and Alberto Gianoli 3 1 Erasmus Graduate School of Social Science and Humanities (EGSH), Erasmus University, 3062 PA Rotterdam, The Netherlands 2 Erasmus School of Social and Behavioural Sciences (ESSB), Erasmus University, 3062 PA Rotterdam, The Netherlands; [email protected] 3 Institute for Housing and Urban Development Studies (IHS), Erasmus University, 3062 PA Rotterdam, The Netherlands; [email protected] * Correspondence: [email protected] Received: 5 October 2019; Accepted: 4 November 2019; Published: 6 November 2019 Abstract: One positive impact of smart cities is reducing energy consumption and CO2 emission through the use of information and communication technologies (ICT). Energy transition pursues systematic changes to the low-carbon society, and it can benefit from technological and institutional advancement in smart cities. The integration of the energy transition to smart city development has not been thoroughly studied yet. The purpose of this study is to find empirical evidence of smart cities’ contributions to energy transition. The hypothesis is that there is a significant difference between smart and non-smart cities in the performance of energy transition. The Smart Energy Transition Index is introduced. Index is useful to summarize the smart city component’s contribution to energy transition and to enable comparison among cities. The cities in South Korea are divided into three groups: (1) first-wave smart cities that focus on smart transportation and security services; (2) second-wave smart cities that provide comprehensive urban services; and (3) non-smart cities. The results showed that second-wave smart cities scored higher than first-wave and non-smart cities, and there is a statistically significant difference among city groups.
    [Show full text]
  • Downloads/Contract Application/Epik Contract.Pdf
    1 The Usefulness of Decision-Forcing Case Studies in Helping to Prepare New and Experienced English as Foreign Language Teachers Brent W. Crofton Concordia University Portland An Action Research Report Presented to The Graduate Program in Partial Fulfillment of the Requirements For the Degree of Masters in Education Concordia University Portland 2011 2 Abstract The objective of this action research project was to find the usefulness of decision-forcing case studies in helping to prepare new and experienced English as foreign language teachers (EFL) taking place in Western Chungnam province of South Korea. Nine decision-forcing cases were written for two Korean English Teachers (KETs) and two Native English Speakers (NESs) with one of each experienced or new to EFL teaching. Following the cases, participants were interviewed and, after assessing the cases, a follow-up by the participants that ranked the decision-forcing cases either “one” or “three.” An average of five cases were determined to be decision-forcing for the four participants. Answers to the decision-forcing cases revealed thoughts, feelings, and actions that participants believed they would experience in each scenario. This study concluded that decision-forcing cases are helpful to KETs and NESs because the situations may happen in any classroom or anywhere in Korea, and, thus, they provide information about cross-cultural communication issues, culture, English pragmatics, language, lessons that fail, and racism. 3 Dedication I unconditionally love and thank my wife Katherine, son Owen, and daughter Edith for enduring all the struggles to complete this study. I want to thank Dr. Bianca Elliott for her patience, belief in me, and vision for what this study has evolved to become.
    [Show full text]
  • Quantitative Determination Procedures for Regional Extreme Drought Conditions: Application to Historical Drought Events in South Korea
    atmosphere Article Quantitative Determination Procedures for Regional Extreme Drought Conditions: Application to Historical Drought Events in South Korea Chan Wook Lee 1 , Moo Jong Park 2 and Do Guen Yoo 1,* 1 Department of Civil Engineering, University of Suwon, Hwaseong-si, Gyeonggi-do 445-743, Korea; [email protected] 2 Department of Aeronautics and Civil Engineering, Hanseo University, Seosan 31962, Korea; [email protected] * Correspondence: [email protected]; Tel.: +82-2-5386-9052 Received: 30 March 2020; Accepted: 27 May 2020; Published: 2 June 2020 Abstract: Recently, the signs of extreme droughts, which were thought of as exceptional and unlikely, are being detected worldwide. It is necessary to prepare countermeasures against extreme droughts; however, current definitions of extreme drought are just used as only one or two indicators to represent the status or severity of a drought. More representative drought factors, which can show the status and severity that are relevant to extreme drought, need to be considered depending on the characteristics of the drought and comprehensive evaluation of various indices. Therefore, this study attempted to quantitatively define regional extreme droughts using more acceptable factors. The methodology comprises five factors that are indicative of extreme drought. The five factors are (1) duration (days), (2) number of consecutive years (years), (3) water availability, (4) return period, and (5) regional experience. The results were analyzed by applying the procedure to droughts that took place in 2014–2015 in South Korea. The results showed that the applied historical event did not enter the status of extreme drought, which is proposed in this study; however, the proposed methodology is applicable because it uses acceptable and reasonable factors to judge extreme drought, but it can also take into account the past regional experience of extreme drought.
    [Show full text]