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Disertacija -- Saulius Alijosius -- for WEB.Pdf LIETUVOS SVEIKATOS MOKSLŲ UNIVERSITETAS VETERINARIJOS AKADEMIJA Saulius Alijošius SKIRTINGŲ MIGLINIŲ ŠEIMOS JAVŲ RŪŠIŲ IR VEISLIŲ GRŪDŲ MAISTINĖS VERTĖS ANALIZĖ IR NEKRAKMOLO POLISACHARIDUS SKAIDANČIŲ FERMENTŲ BEI MANANOOLIGOSACHARIDŲ PANAUDOJIMO TYRIMAI VIŠTŲ DEDEKLIŲ IR VIŠČIUKŲ BROILERIŲ LESALUOSE Daktaro disertacija Žemės ūkio mokslai, zootechnika (03A) Kaunas, 2017 1 Disertacija rengta 2013–2017 metais Lietuvos sveikatos mokslų universiteto Veterinarijos akademijoje, Gyvūnų auginimo technologijų institute. Mokslinis vadovas – prof. habil. dr. Romas Gružauskas (Lietuvos sveikatos mokslų univer- sitetas, žemės ūkio mokslai, zootechnika – 03A). Disertacija ginama Lietuvos sveikatos mokslų universiteto Zootechnikos mokslo krypties taryboje: Pirmininkė – prof. dr. Elena Bartkienė (Lietuvos sveikatos mokslų universitetas, žemės ūkio mokslai, zootechnika – 03A). Nariai: dr. Jonas Jatkauskas (Lietuvos sveikatos mokslų universitetas, žemės ūkio mokslai, zootechnika – 03A); doc. dr. Antanas Šarkinas (Kauno technologijos universitetas, technolo- gijos mokslai, chemijos inžinerija – 05T); prof. dr. Gintarė Zaborskienė (Lietuvos sveikatos mokslų universitetas, žemės ūkio mokslai, zootechnika – 03A); prof. dr. Qendrim Zebeli (Vienos veterinarinės medicinos universitetas, žemės ūkio mokslai, veterinarija – 02A). Disertacija ginama viešajame Zootechnikos mokslo krypties tarybos posėdyje 2017 m. gruodžio 20 d. 13 val. Lietuvos sveikatos mokslų universiteto Veterinarijos akademijos Dr. S. Jankausko auditorijoje. Disertacijos gynimo vietos adresas: Tilžės g. 18, LT-47181 Kaunas, Lietuva. 2 LITHUANIAN UNIVERSITY OF HEALTH SCIENCES VETERINARY ACADEMY Saulius Alijošius NUTRITIVE VALUE ANALYSIS OF CEREAL GRAIN (FAMILY POACEAE) SPECIES AND VARIETIES, USAGE INVESTIGATION OF NON STARCH POLYSACCHARIDES DEGRADING ENZYMES, MANANOOLIGOSACCHARIDES IN LAYING HENS AND BROILER CHICKENS FEEDS Doctoral Dissertation Agricultural Sciences, Zootechnics (03A) Kaunas, 2017 3 Dissertation has been prepared at the Institute of Animal Rearing Technologies of Veterinary Academy of Lithuanian University of Health Sciences during the period of 2013–2017. Scientific supervisor – Prof. Habil. Dr. Romas Gružauskas (Lithuanian University of Health Sciences, Agricultural Sciences, Zootechnics – 03A). Dissertation is defended at the Zootechnics Research Council of Lithuanian University of Health Sciences. Chairperson – Prof. Dr. Elena Bartkienė (Lithuanian University of Health Sciences, Agricultural Sciences, Zootechnics – 03A). Members: Dr. Jonas Jatkauskas (Lithuanian University of Health Sciences, Agricultural Sciences, Zootechnics – 03A); Assoc. Prof. Dr. Antanas Šarkinas (Kaunas University of Technology, Technological Sciences, Chemical Engineering – 05T); Prof. Dr. Gintarė Zaborskienė (Lithuanian University of Health Sciences, Agricultural Sciences, Zootechnics – 03A); Prof. Dr. Qendrim Zebeli (University of Veterinary Medicine, Vienna, Agricultural Sciences, Veterinary – 02A). Dissertation will be defended at the open session of the Lithuanian University of Health Sciences on the 20th of December, 2017 at 1:00 p.m. in Dr. S. Jan- kauskas Auditorium of the Veterinary Academy. Address: Tilžės 18, LT-47181 Kaunas, Lithuania. 4 TURINYS SANTRUMPOS .................................................................................................................... 7 ĮVADAS ................................................................................................................................ 9 1. LITERATŪROS APŽVALGA ...................................................................................... 12 1.1. Javų grūdų cheminės sudėties ir maistinės vertės analizė ..................................... 12 1.1.1. Angliavandeniai ....................................................................................... 12 1.1.2. Baltymai .................................................................................................. 13 1.1.3. Krakmolas ............................................................................................... 13 1.1.4. Riebalai .................................................................................................... 14 1.2. Nekrakmolo polisacharidai .................................................................................... 14 1.2.1. Ligninas ................................................................................................... 15 1.2.2. Celiuliozė ................................................................................................. 15 1.2.3. Hemiceliuliozės ....................................................................................... 16 1.2.4. Arabinoksilanai ........................................................................................ 16 1.2.5. Beta gliukanai .......................................................................................... 18 1.3. Nekrakmolo polisacharidus skaidančių fermentų įtaka paukščių produktyvumui, virškinimo procesams bei produkcijos kokybei ..................................................... 19 1.4. Prebiotikai ir jų įtaka paukščių produktyvumui, virškinimo procesams bei produkcijos kokybei .............................................................................................. 22 1.5. Paukščių lesaluose esančių maisto medžiagų virškinimas ir plonųjų žarnų histologija .............................................................................................................. 24 2. TYRIMO MEDŽIAGOS IR METODAI ....................................................................... 27 2.1. Tyrimų vieta, laikas, objektas ir tyrimo etapai ...................................................... 27 2.2. Paukščių laikymas ir lesinimas .............................................................................. 29 2.3. Bandymų su paukščiais schemos ........................................................................... 29 2.3.1. Medžiagų apykaitos bandymai su viščiukais broileriais .......................... 29 2.3.2. Lesinimo bandymas su vištomis dedeklėmis ........................................... 30 2.3.3. Lesinimo bandymas su viščiukais broileriais .......................................... 31 2.4. Tirtųjų lesalų priedų charakteristikos .................................................................... 32 2.5. Lesalų gamyba ir jų sudėtis ................................................................................... 33 2.6. Tyrimų metodai ..................................................................................................... 33 2.6.1. Skirtingų javų rūšių ir veislių grūdų maistinės vertės tyrimų metodai ... 33 2.6.2. Medžiagų apykaitos bandymų su viščiukais broileriais tyrimų metodai .................................................................................................... 37 2.6.3. Lesinimo bandymo su vištomis dedeklėmis tyrimų metodai ................... 38 2.6.3.1. Zootechniniai tyrimų metodai ................................................................. 38 2.6.3.2. Fiziologiniai tyrimų metodai ................................................................... 38 2.6.3.3. Kiaušinių kokybės tyrimų metodai .......................................................... 39 2.6.3.4. Kiaušinių tekstūrinių ir juslinių savybių tyrimo metodai ........................ 40 5 2.6.4. Lesinimo bandymo su viščiukais broileriais tyrimų metodai .................. 41 2.6.4.1. Zootechniniai tyrimų metodai ................................................................. 41 2.6.4.2. Fiziologiniai tyrimų metodai ................................................................... 42 2.6.4.3. Viščiukų broilerių mėsos kokybės tyrimo metodai ................................. 43 2.6.4.4. Viščiukų broilerių mėsos juslinių savybių tyrimo metodai ..................... 44 2.7. Statistinė duomenų analizė .................................................................................... 46 3. TYRIMŲ REZULTATAI .............................................................................................. 47 3.1. Skirtingų javų veislių grūdų cheminės sudėties rezultatai ..................................... 47 3.2. Medžiagų apykaitos bandymų su viščiukais broileriais rezultatai ......................... 69 3.3. Lesinimo bandymo su vištomis dedeklėmis rezultatai .......................................... 71 3.4. Lesinimo bandymo su viščiukais broileriais rezultatai .......................................... 87 4. TYRIMO REZULTATŲ APTARIMAS ..................................................................... 103 IŠVADOS .......................................................................................................................... 110 REKOMENDACIJOS ....................................................................................................... 112 LITERATŪROS ŠALTINIAI ........................................................................................... 113 PASKELBTOS PUBLIKACIJOS ..................................................................................... 129 SUMMARY....................................................................................................................... 151 PRIEDAI ........................................................................................................................... 192 CURRICULUM VITAE .................................................................................................... 211 PADĖKA ........................................................................................................................... 212 6
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