DOCSLIB.ORG

Development and Application of Molecular Markers to Maerl‐Forming Species in Europe: Data for Their Conservation

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Development and Application of Molecular Markers to Maerl‐Forming Species in Europe: Data for Their Conservation Development and applicaon of molecular markers to maerl-forming species in Europe: data for their conservaon PhD thesis Crisna Pardo Carabias 2016 Departamento de Bioloxía Animal, Bioloxía Vexetal e Ecoloxía Facultade de Ciencias Development and application of molecular markers to maerl‐forming species in Europe: data for their conservation Desarrollo y aplicación de marcadores moleculares a especies formadoras de maerl en Europa: datos para su conservación Desenvolvemento e aplicación de marcadores moleculares a especies formadoras de maerl en Europa: datos para a súa conservación PhD Thesis / Tesis de Doctorado / Tese de Doutoramento Cristina Pardo Carabias Supervisors / Directores / Directores: Dr. Ignacio M. Bárbara Criado Dr. Rodolfo Barreiro Lozano Dr. Viviana Peña Freire Tutor / Tutor / Titor: Dr. Rodolfo Barreiro Lozano Reviewed by / Revisado por / Revisado por: Dr. Jacques Grall (Observatoire du Domaine Côtier de l'IUEM‐OSU, France) Dr. Rafael Riosmena Rodríguez (Universidad Autónoma de Baja California Sur, México) Deposit / Depósito / Depósito: October / Octubre / Outubro 2015 Defense / Defensa / Defensa: January / Enero / Xaneiro 2016 Departamento de Bioloxía Animal, Bioloxía Vexetal e Ecoloxía. Facultade de Ciencias da UDC. Programa Oficial de Doutoramento en Acuicultura (Interuniversitario). RD 1393/2007. i IGNACIO M. BÁRBARA CRIADO, RODOLFO BARREIRO LOZANO AND VIVIANA PEÑA FREIRE, SENIOR LECTURER OF BOTANY, PROFESSOR OF ECOLOGY AND POSTDOCTORAL RESEARCHER, RESPECTIVELY, FROM DEPARTMENT OF ANIMAL BIOLOGY, PLANT BIOLOGY AND ECOLOGY OF THE UNIVERSITY OF A CORUÑA CERTIFY: That the following report entitled "Development and application of molecular markers to maerl‐forming species in Europe: data for their conservation" written by Miss CRISTINA PARDO CARABIAS has been prepared under their supervision in the Department of Animal Biology, Plant Biology and Ecology at the Science Faculty of the University of A Coruña, within the framework of the Official PhD Program in Aquaculture (Inter) regulated by RD 1393/2007, and it meets the requirements to be defended and to aspire to the degree of “International PhD”. And for any legal statement, the present document is signed in A Coruña October 8, 2015. Dr. Ignacio M. Bárbara Criado Dr. Rodolfo Barreiro Lozano Dr. Viviana Peña Freire iii IGNACIO M. BÁRBARA CRIADO, RODOLFO BARREIRO LOZANO Y VIVIANA PEÑA FREIRE, PROFESOR TITULAR DE BOTÁNICA, CATEDRÁTICO DE ECOLOGÍA Y CONTRATADA POSTDOCTORAL (XUNTA DE GALICIA MODALIDAD A), RESPECTIVAMENTE, DEL DEPARTAMENTO DE BIOLOGÍA ANIMAL, BIOLOGÍA VEGETAL Y ECOLOGÍA DE LA UNIVERSIDAD DE A CORUÑA DECLARAN: Que la siguiente memoria titulada “Development and application of molecular markers to maerl‐forming species in Europe: data for their conservation” presentada por Doña CRISTINA PARDO CARABIAS ha sido realizada bajo su dirección en el Departamento de Biología Animal, Biología Vegetal y Ecología de la Facultad de Ciencias de la Universidad de A Coruña, dentro del Programa Oficial de Doctorado en Acuicultura (Interuniversitario) regulado por el RD 1393/2007, y cumple con las condiciones exigidas para ser defendido y optar al grado de “Doctora Internacional” ante el tribunal que lo deberá juzgar. Y para que así conste a los efectos oportunos, firman la presente en A Coruña a 8 de Octubre de 2015. Dr. Ignacio M. Bárbara Criado Dr. Rodolfo Barreiro Lozano Dra. Viviana Peña Freire v IGNACIO M. BÁRBARA CRIADO, RODOLFO BARREIRO LOZANO E VIVIANA PEÑA FREIRE, PROFESOR TITULAR DE BOTÁNICA, CATEDRÁTICO DE ECOLOXÍA E CONTRATADA POSDOUTORAL (XUNTA DE GALICIA MODALIDADE A), RESPECTIVAMENTE, DO DEPARTAMENTO DE BIOLOXÍA ANIMAL, BIOLOXÍA VEXETAL E ECOLOXÍA DA UNIVERSIDADE DA CORUÑA DECLARAN: Que a seguinte memoria titulada “Development and application of molecular markers to maerl‐forming species in Europe: data for their conservation” presentada por Dona CRISTINA PARDO CARABIAS foi realizada baixo a súa dirección no Departamento de Bioloxía Animal, Bioloxía Vexetal e Ecoloxía da Facultade de Ciencias da Universidade da Coruña, dentro do Programa Oficial de Doutoramento en Acuicultura (Interuniversitario) regulado polo RD 1393/2007, e cumpre coas condicións esixidas para ser defendido e optar ao grao de “Doutora Internacional” ante o tribunal que o deberá xulgar. E para que así conste aos efectos oportunos, asinan a presente na Coruña a 8 de Outubro de 2015. Dr. Ignacio M. Bárbara Criado Dr. Rodolfo Barreiro Lozano Dra. Viviana Peña Freire vii This PhD thesis was funded by the projects CTM2010‐18787 (Ministerio de Ciencia e Innovación del Gobierno de España, partially funded by ERDF‐the European Regional Development Fund‐) and 10MMA103003PR (Xunta de Galicia). Sampling in Brittany (France) was supported by a European Community‐Research Infrastructure Action under the FP7 ‘‘Capacities’’ Specific Programme (ASSEMBLE grant agreement no. 227799). Cristina Pardo Carabias appreciates the fellowships received to carry out the present PhD thesis in “Universidade da Coruña”: ‐ “Axuda de apoio á etapa predoutoral do Plan Galego de Investigación, Innovación e Crecemento 2011‐2015 (Plan I2C) para o ano 2011 da Consellería de Cultura, Educación e Ordenación Universitaria da Xunta de Galicia. Expediente PRE/2011/058” from November 1st, 2011 to December 20th, 2012. ‐ “Beca del Programa de Formación del Profesorado Universitario (FPU). Convocatoria 2010. Nuevas becas del Ministerio de Educación del Gobierno de España. Referencia AP2010‐1682” from December 21st, 2012 to October 31st, 2015. Cristina Pardo Carabias also appreciates a scholarship to carry out a research stay in the “Station Biologique de Roscoff” (France) from September 15th 2014 to 15th December 2014 under the supervision of Dr. Myriam Valero: ‐ “Ayuda del Programa de Formación del Profesorado Universitario (FPU). Convocatoria 2013. Estancias breves del Ministerio de Educación, Cultura y Deporte del Gobierno de España. Referencia EST13/00886”. ix To my parents, my sisters and my nephew, who have always been there. A mis padres, mis hermanas y mi sobrino, que siempre han estado ahí. Aos meus pais, as miñas irmás e o meu sobriño, que sempre estiveron aí. xi ACKNOWLEDGMENTS I would like to thank my PhD supervisors Dr. Ignacio M. Bárbara, Dr. Rodolfo Barreiro and Dr. Viviana Peña for their inestimable support and help during this thesis. I want to thank all my partners of the BIOCOST Research Group for their help, support and shared moments during this thesis: Javier Cremades, Pilar Díaz, Juan Lugilde, Alicia García, Verónica García, J. Miguel Ruíz, Sergio R. Roiloa, Cristina Piñeiro, Pablo Touriñán, José Ribamar, María Quintela, Lucía Couceiro, Naiara Albaina and Isabel Maneiro. In addition, I want to thank Belén Carro and Lúa López that without their inestimable help and support, this thesis would not have been possible. I am grateful to Dr. Myriam Valero for welcoming me in her Research Group during my stay in Roscoff (Brittany) and all people of her Research Group. Also thanks to all people that during my stay encouraged and help me: Alejandro Montecinos, Katherine Valenzuela, Zujaila Qui Minet, Marie Rescan, Jérome Coudret, Komlan Avia and Marie‐Laure Guillemin. I am also grateful to Dr. Christophe Destombe for his invaluable advice and for giving me the chance to meet Dr. Jacqueline Cabioch. Madame Cabioch, thanks for sharing with me your knowledge and by your great advices in maerl beds. My friends and University partners have always supported and inspired me: Laura Sanjurjo, Rafael Carballeira, Teresa Martínez, Esther Novo, Tamara Rábade, Tania García, Marta Lois, Ana B. Monteagudo, Alba García and Irene Lema. In addition, I would like to thank my old friends Raquel Abal, Iria Rodríguez, Miguel Delgado and Fátima Prol for all the moments that they shared with me, thanks guys. Besides, I would like to highlight the company, support and help from my comrade Javier Veloso during my thesis journey and life. Finally, I thank my family for their company and support: my parents Mercedes and Emilio J., my sisters Mónica and Rosa, my nephew Hugo, my uncles Teresa and Luís, my cousins Silvia and Fernando, my godparents Suso and Ana, and my grandparents Emilia and Delfín that although they are no longer here they taught me the best of life. xiii ABSTRACT Appropriate management of habitats of conservation concern, such as the maerl beds, requires reliable information on basic issues like species diversity, life cycle, and the population genetics of maerl‐forming species. Conventionally, these topics had been addressed with morphological information. In comparison, the present thesis has tried to step forward by using molecular markers to solve these gaps in our knowledge. In chapter 1, maerl‐forming species were delimited in Atlantic Europe using an integrative taxonomic approach that included COI‐5P and psbA sequences as molecular data. A new major maerl‐forming species was discovered by DNA barcodes, and its description is provided in chapter 2. A contribution to disentangle the life cycle of a major maerl‐forming species (Phymatolithon calcareum) was obtained with a combination of DNA barcodes and morphology in chapter 3. Chapter 4 describes the development of a set of microsatellite markers for this species using NGS technology. Finally, preliminary results obtained with these microsatellites to several populations of P. calcareum across European Atlantic maerl beds are shown in a final Annex to this thesis. xv RESUMEN La gestión adecuada de los hábitats de interés para la conservación, como los bancos de
Load more

Recommended publications
  • Polar Coralline Algal Caco3-Production Rates Correspond to Intensity and Duration of the Solar Radiation
    Biogeosciences, 11, 833–842, 2014 Open Access www.biogeosciences.net/11/833/2014/ doi:10.5194/bg-11-833-2014 Biogeosciences © Author(s) 2014. CC Attribution 3.0 License. Polar coralline algal CaCO3-production rates correspond to intensity and duration of the solar radiation S. Teichert1 and A. Freiwald2 1GeoZentrum Nordbayern, Section Palaeontology, Erlangen, Germany 2Senckenberg am Meer, Section Marine Geology, Wilhelmshaven, Germany Correspondence to: S. Teichert ([email protected]) Received: 5 July 2013 – Published in Biogeosciences Discuss.: 26 August 2013 Revised: 8 January 2014 – Accepted: 8 January 2014 – Published: 11 February 2014 Abstract. In this study we present a comparative quan- decrease water transparency and hence light incidence at the tification of CaCO3 production rates by rhodolith-forming four offshore sites. Regarding the aforementioned role of the coralline red algal communities situated in high polar lat- rhodoliths as ecosystem engineers, the impact on the associ- itudes and assess which environmental parameters control ated organisms will presumably also be negative. these production rates. The present rhodoliths act as ecosys- tem engineers, and their carbonate skeletons provide an im- portant ecological niche to a variety of benthic organisms. The settings are distributed along the coasts of the Sval- 1 Introduction bard archipelago, being Floskjeret (78◦180 N) in Isfjorden, Krossfjorden (79◦080 N) at the eastern coast of Haakon VII Coralline red algae are the most consistently and heavily Land, Mosselbukta (79◦530 N) at the eastern coast of Mossel- calcified group of the red algae, and as such have been el- halvøya, and Nordkappbukta (80◦310 N) at the northern coast evated to ordinal status (Corallinales Silva and Johansen, of Nordaustlandet.
    [Show full text]
  • Aragonite Infill in Overgrown Conceptacles of Coralline Lithothamnion Spp
    J. Phycol. 52, 161–173 (2016) © 2016 Phycological Society of America DOI: 10.1111/jpy.12392 ARAGONITE INFILL IN OVERGROWN CONCEPTACLES OF CORALLINE LITHOTHAMNION SPP. (HAPALIDIACEAE, HAPALIDIALES, RHODOPHYTA): NEW INSIGHTS IN BIOMINERALIZATION AND PHYLOMINERALOGY1 Sherry Krayesky-Self,2 Joseph L. Richards University of Louisiana at Lafayette, Lafayette, Louisiana 70504-3602, USA Mansour Rahmatian Core Mineralogy Inc., Lafayette, Louisiana 70506, USA and Suzanne Fredericq University of Louisiana at Lafayette, Lafayette, Louisiana 70504-3602, USA New empirical and quantitative data in the study of calcium carbonate biomineralization and an All crustose coralline algae belonging in the Coral- expanded coralline psbA framework for linales, Hapalidiales, and Sporolithales (Rhodo- phylomineralogy are provided for crustose coralline phyta) are characterized by the presence of calcium red algae. Scanning electron microscopy (SEM) and carbonate in their cell walls, which is often in the energy dispersive spectrometry (SEM-EDS) form of highly soluble high-magnesium-calcite (Adey pinpointed the exact location of calcium carbonate 1998, Knoll et al. 2012, Adey et al. 2013, Diaz-Pulido crystals within overgrown reproductive conceptacles et al. 2014, Nelson et al. 2015). Besides the in rhodolith-forming Lithothamnion species from the Rhodogorgonales (Fredericq and Norris 1995), a sis- Gulf of Mexico and Pacific Panama. SEM-EDS and ter group of the coralline algae in the Corallinophy- X-ray diffraction (XRD) analysis confirmed the cidae (Le Gall and Saunders 2007) whose members elemental composition of these calcium carbonate also precipitate calcite, all other calcified red and crystals to be aragonite. After spore release, green macroalgae deposit calcium carbonate in the reproductive conceptacles apparently became form of aragonite (reviewed in Adey 1998, Nelson overgrown by new vegetative growth, a strategy that 2009).
    [Show full text]
  • Arctic Rhodolith Beds and Their Environmental Controls (Spitsbergen, Norway)
    Facies (2014) 60:15–37 DOI 10.1007/s10347-013-0372-2 ORIGINAL ARTICLE Arctic rhodolith beds and their environmental controls (Spitsbergen, Norway) S. Teichert • W. Woelkerling • A. Ru¨ggeberg • M. Wisshak • D. Piepenburg • M. Meyerho¨fer • A. Form • A. Freiwald Received: 10 December 2012 / Accepted: 18 April 2013 / Published online: 10 May 2013 Ó Springer-Verlag Berlin Heidelberg 2013 Abstract Coralline algae (Corallinales, Rhodophyta) that corallines are thriving and are highly specialized in their form rhodoliths are important ecosystem engineers and adaptations to the physical environment as well as in their carbonate producers in many polar coastal habitats. This interaction with the associated benthic fauna, which is study deals with rhodolith communities from Floskjeret similar to other polar rhodolith communities. The marine (78°180N), Krossfjorden (79°080N), and Mosselbukta environment of Spitsbergen is already affected by a cli- (79°530N), off Spitsbergen Island, Svalbard Archipelago, mate-driven ecological regime shift and will lead to an Norway. Strong seasonal variations in temperature, salin- increased borealization in the near future, with presently ity, light regime, sea-ice coverage, and turbidity charac- unpredictable consequences for coralline red algal terize these localities. The coralline algal flora consists of communities. Lithothamnion glaciale and Phymatolithon tenue. Well- developed rhodoliths were recorded between 27 and 47 m Keywords Depth gradient Á Environmental parameters Á water depth, while coralline algal encrustations on litho- Lithothamnion glaciale Á Phymatolithon tenue Á clastic cobbles were detected down to 77 m water depth. At Rhodolith community Á Seasonality Á Spitsbergen all sites, ambient waters were saturated with respect to both aragonite and calcite, and the rhodolith beds were located predominately at dysphotic water depths.
    [Show full text]
  • Rhodoliths and Rhodolith Beds Michael S
    Rhodoliths and Rhodolith Beds Michael S. Foster, Gilberto M. Amado Filho, Nicholas A. Kamenos, Rafael Riosmena- Rodríguez, and Diana L. Steller ABSTRACT. Rhodolith (maërl) beds, communities dominated by free living coralline algae, are a common feature of subtidal environments worldwide. Well preserved as fossils, they have long been recognized as important carbonate producers and paleoenvironmental indicators. Coralline algae produce growth bands with a morphology and chemistry that record environmental varia- tion. Rhodoliths are hard but often fragile, and growth rates are only on the order of mm/yr. The hard, complex structure of living beds provides habitats for numerous associated species not found on otherwise entirely sedimentary bottoms. Beds are degraded locally by dredging and other an- thropogenic disturbances, and recovery is slow. They will likely suffer severe impacts worldwide from the increasing acidity of the ocean. Investigations of rhodolith beds with scuba have enabled precise stratified sampling that has shown the importance of individual rhodoliths as hot spots of diversity. Observations, collections, and experiments by divers have revolutionized taxonomic stud- ies by allowing comprehensive, detailed collection and by showing the large effects of the environ- ment on rhodolith morphology. Facilitated by in situ collection and calibrations, corallines are now contributing to paleoclimatic reconstructions over a broad range of temporal and spatial scales. Beds are particularly abundant in the mesophotic zone of the Brazilian shelf where technical diving has revealed new associations and species. This paper reviews selected past and present research on rhodoliths and rhodolith beds that has been greatly facilitated by the use of scuba. Michael S. Foster, Moss Landing Marine Labo- INTRODUCTION ratories, 8272 Moss Landing Road, Moss Land- ing, California 95039, USA.
    [Show full text]
  • Abstract Book
    Abstract book VI International Rhodolith Workshop Roscoff, France 25-29 June 2018 Table of contents Taxonomy 6 Molecular systematics: toward understanding the diversity of Corallinophyci- dae, Viviana Pena...................................7 Simplified coralline specimens' DNA preparation, mini barcoding & HRM analysis targeting a short psbA section, Marc Angl`esD'auriac [et al.]...........8 Reassessment of Lithophyllum kotschyanum and L. okamurae in the North-Western Pacific Ocean, Aki Kato [et al.]............................9 Phymatolithopsis gen. nov. (Hapalidiaceae, Rhodophyta) based on molecular and morphological evidence, So Young Jeong [et al.]................ 10 Morpho-anatomical descriptions and DNA sequencing of the species of the genus Porolithon occurring in the Great Barrier Reef, Alexandra Ord´o~nez[et al.].... 11 Ecophysiology 12 How do rhodoliths get their energy?, Laurie Hofmann............... 13 Effect of seawater carbonate chemistry and other environmental drivers on the calcification physiology of two rhodoliths, Steeve Comeau [et al.]......... 14 Short- and long-term effects of high CO2 on the photosynthesis and calcification of the free-living coralline algae Phymatolithon lusitanicum, Jo~aoSilva [et al.].. 15 Physiological responses of tropical (Lithophyllum pygmaeum) and temperate (Coral- lina officinalis) branching coralline algae to future climate change conditions, Bon- nie Lewis [et al.].................................... 16 Role of evolutionary history in the responses of tropical crustose coralline algae to ocean acidification, Guillermo Diaz-Pulido [et al.]................ 17 1 Coralline algal recruits gain tolerance to ocean acidification over successive gen- erations of exposure, Christopher E. Cornwall [et al.]................ 18 Rhodolith communities in a changing ocean: species-specific responses of Brazil- ian subtropical rhodoliths to global and local stressors, Nadine Schubert [et al.]. 19 Maerl bed community physiology is impacted by elevated CO2, Heidi Burdett [et al.]...........................................
    [Show full text]
  • Phymatolithon (Melobesioideae, Hapalidiales) in the Boreal–Subarctic • Number 41 Transition Zone of The
    Adey et al. Smithsonian Institution Scholarly Press smithsonian contributions to the marine sciences • number 41 Smithsonian Institution Smithsonian Contributions to the Marine Sciences Scholarly Press Phymatolithon (Melobesioideae, Hapalidiales) in the Boreal–Subarctic • Number 41 Transition Zone of the North Atlantic A Correlation of Plastid DNA Markers with Morpho-Anatomy, Ecology, and Biogeography Walter H. Adey, Jazmin J. Hernandez-Kantun, 2018 Paul W. Gabrielson, Merinda C. Nash, and Lee-Ann C. Hayek SERIES PUBLICATIONS OF THE SMITHSONIAN INSTITUTION Emphasis upon publication as a means of “diffusing knowledge” was expressed by the first Secretary of the Smithsonian. In his formal plan for the Institution, Joseph Henry outlined a program that included the following statement: “It is proposed to publish a series of reports, giving an account of the new discoveries in science, and of the changes made from year to year in all branches of knowledge.” This theme of basic research has been adhered to through the years in thousands of titles issued in series publications under the Smithsonian imprint, commencing with Smithsonian Contributions to Knowledge in 1848 and continuing with the following active series: Smithsonian Contributions to Anthropology Smithsonian Contributions to Botany Smithsonian Contributions to History and Technology Smithsonian Contributions to the Marine Sciences Smithsonian Contributions to Museum Conservation Smithsonian Contributions to Paleobiology Smithsonian Contributions to Zoology In these series, the Smithsonian Institution Scholarly Press (SISP) publishes small papers and full-scale monographs that report on research and collections of the Institution’s museums and research centers. The Smithsonian Contributions Series are distributed via exchange mailing lists to libraries, universities, and similar institutions throughout the world.
    [Show full text]
  • New Records of Rhodolith-Forming Species (Corallinales, Rhodophyta) from Deep Water in Espırito Santo State, Brazil
    Helgol Mar Res (2012) 66:219–231 DOI 10.1007/s10152-011-0264-1 ORIGINAL ARTICLE New records of rhodolith-forming species (Corallinales, Rhodophyta) from deep water in Espı´rito Santo State, Brazil Maria Carolina Henriques • Alexandre Villas-Boas • Rafael Riosmena Rodriguez • Marcia A. O. Figueiredo Received: 20 August 2010 / Revised: 30 May 2011 / Accepted: 5 July 2011 / Published online: 17 July 2011 Ó Springer-Verlag and AWI 2011 Abstract Little is known about the diversity of non- Introduction geniculate coralline red algae (Rhodophyta, Coral- linophycidae) from deep waters in Brazil. Most surveys ‘‘Rhodolith’’ is the name given to free-living structures undertaken in this country have been carried out in shallow composed mostly ([50%) of non-geniculate coralline red waters. In 1994, however, the REVIZEE program surveyed algae (Rhodophyta, Corallinophycidae). The communities the sustainable living resources potential of the Brazilian they denominate are called ‘‘rhodolith beds’’ (Foster 2001; exclusive economic zone to depths of 500 m. In the present Harvey and Woelkerling 2007). However, there is no study, the rhodolith-forming coralline algae from the con- consensus on use of the terms (Foster 2001). In this study, tinental shelf of Espı´rito Santo State were identified. we follow Foster (2001) and Harvey and Woelkerling Samples were taken from 54 to 60 m depth by dredging (2007). during ship cruises in 1997. Three rhodolith-forming spe- The deepest known alga is a rhodolith-forming coralline cies were found: Spongites yendoi (Foslie) Chamberlain, red algal species discovered on an uncharted seamount at Lithothamnion muelleri Lenormand ex Rosanoff and 268 m depth in the Bahamas (Littler et al.
    [Show full text]
  • Diretrizes Para Auxílio Na Confecção De
    Talita Vieira-Pinto DIVERSIDADE DAS ALGAS CALCÁRIAS CROSTOSAS DO BRASIL BASEADA EM MARCADORES MOLECULARES E MORFOLOGIA DIVERSITY OF CRUSTOSE CORALLINE ALGAE FROM BRAZIL BASED ON MOLECULAR MARKERS AND MORPHOLOGY São Paulo, SP 2016 Talita Vieira-Pinto DIVERSIDADE DAS ALGAS CALCÁRIAS CROSTOSAS DO BRASIL BASEADA EM MARCADORES MOLECULARES E MORFOLOGIA DIVERSITY OF CRUSTOSE CORALLINE ALGAE FROM BRAZIL BASED ON MOLECULAR MARKERS AND MORPHOLOGY Tese apresentada ao Instituto de Biociências da Universidade de São Paulo, para a obtenção de Título de Doutor em Ciências, na Área de Botânica. Orientador(a): Mariana Cabral de Oliveira Colaborador(a): Suzanne Fredericq São Paulo, SP 2016 Vieira-Pinto, Talita Diversity of Crustose Coralline Algae (CCA) from Brazil based on molecular markers and morphology Número de páginas Tese (Doutorado) - Instituto de Biociências da Universidade de São Paulo. Departamento de Botânica, 2016. 1. Crostose coralline algae 2. DNA 3. Taxonomy I. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica. Comissão Julgadora: Prof(a). Dr(a). Prof(a). Dr(a). Prof(a). Dr(a). Prof(a). Dr(a). Prof(a). Dr(a). Orientador(a) Dedication To my family; To honor the memory of Dr. Rafael Riosmena-Rodriguez Acknowledgments I would like to thank Fundação de Amparo à Pesquisa do Estado de São Paulo – FAPESP for providing me two scholarships (Proc. FAPESP 2012/0507-6 and 2014/13386-7) and for funding our research and courses and meetings I attended along these years. I would also like to thank my admirable advisor, Dr. Mariana Cabral de Oliveira for all she did for me and all she does to Brazilian science – you are a true inspiration and a whole model.
    [Show full text]
  • There Is More in Maerl Than Meets
    Melbourne, L., J. Hernández Kantún, J., Russell, S., & Brodie, J. (2017). There is more to maerl than meets the eye: DNA barcoding reveals a new species in Britain, Lithothamnion erinaceum sp. nov. (Hapalidiales, Rhodophyta). European Journal of Phycology, 52(2), 166-178. https://doi.org/10.1080/09670262.2016.1269953 Peer reviewed version License (if available): CC BY Link to published version (if available): 10.1080/09670262.2016.1269953 Link to publication record in Explore Bristol Research PDF-document This is the author accepted manuscript (AAM). The final published version (version of record) is available online via Taylor & Francis at http://www.tandfonline.com/doi/full/10.1080/09670262.2016.1269953. Please refer to any applicable terms of use of the publisher. University of Bristol - Explore Bristol Research General rights This document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/red/research-policy/pure/user-guides/ebr-terms/ There is more to maerl than meets the eye: DNA barcoding reveals a new species in Britain, Lithothamnion erinaceum sp. nov. (Hapalidiales, Rhodophyta) Leanne A. Melbourne1,2, Jazmin J. Hernández-Kantún3, Stephen Russell2 and Juliet Brodie2 1) Department of Earth Sciences, University of Bristol, Wills Memorial Building, Queen’s Road, Bristol, BS8 1RJ, UK 2) Department of Life Sciences, Natural History Museum, Cromwell Road, London, SW7 5BD, UK 3) Department of Botany, National Museum of Natural History, Smithsonian Institution, MRC 166 PO box 37012, Washington, DC, USA Corresponding author: Leanne Melbourne Email: [email protected] Running title: revealing cryptic diversity within Lithothamnion 1 ABSTRACT Due to the high plasticity of coralline algae, identification based on morphology alone can be extremely difficult, so studies increasingly use a combination of morphology and genetics in species delimitation.
    [Show full text]
  • 1 Anatomical Structure Overrides Temperature Controls On
    1 ANATOMICAL STRUCTURE OVERRIDES TEMPERATURE CONTROLS ON 2 MAGNESIUM UPTAKE - CALCIFICATION IN THE ARCTIC/SUBARCTIC 3 CORALLINE ALGAE LEPTOPHYTUM LAEVE AND KVALEYA EPILAEVE 4 (RHODOPHYTA; CORALLINALES) 5 Merinda C. Nash 6 Walter Adey 7 Department of Botany, National Museum of Natural History, Smithsonian Institution, 8 Washington, DC, USA, 20560 9 10 Author for correspondence: [email protected] 11 Running title: Magnesium and anatomy in coralline algae 12 Key words: Coralline algae, calcification, biomineralization, magnesium, temperature, 13 proxy 14 1 15 16 Abstract 17 18 Calcified coralline red algae are ecologically key organisms in photic benthic 19 environments. In recent decades they have become important climate proxies, especially 20 in the Arctic and Subarctic. It has been widely accepted that Magnesium content in 21 coralline tissues is directly a function of ambient temperature, and this is a primary basis 22 for their value as a climate archive. In this paper we show for two genera of 23 Arctic/Subarctic corallines, Leptophytum laeve and Kvaleya epilaeve, that previously 24 unrecognized complex tissue and cell wall anatomy bears a variety of basal signatures for 25 Mg content, with the accepted temperature relationship being secondary. The 26 interfilament carbonate has lower Mg than adjacent cell walls and the hypothallial cell 27 walls have the highest Mg content. The internal structure of the hypothallial cell walls 28 can differ substantially from the perithallial radial cell wall structure. Using high- 29 magnification Scanning Electron Microscopy and etching we expose the nm-scale 30 structures within the cell walls and interfilament. Fibrils concentrate at the internal and 31 external edges of the cell walls.
    [Show full text]
  • A Stable Ultrastructural Pattern Despite Variable Cell Size in Lithothamnion Corallioides
    https://doi.org/10.5194/bg-2021-140 Preprint. Discussion started: 3 August 2021 c Author(s) 2021. CC BY 4.0 License. A stable ultrastructural pattern despite variable cell size in Lithothamnion corallioides Valentina Alice Bracchi1, Giulia Piazza1, Daniela Basso1 5 1Department of Earth and Environmental Sciences, University of Milano-Bicocca, Milan, 20126, Italy Correspondence to: Valentina A. Bracchi ([email protected]) Abstract. Recent advances on the mechanism and pattern of calcification in coralline algae lead to contradictory conclusions. 10 Coralline calcification appears biologically induced, as suggested by the dependency of its elemental composition on environmental variables. However, evidence of a biologically controlled calcification process, resulting in distinctive patterns at the scale of family, was also observed. In order to clarify the matter, five collections of Lithothamnion corallioides from the Atlantic Ocean and the Mediterranean Sea, across a wide depth range (12-66 m) have been analyzed for morphology, anatomy and cell wall crystal patterns of both perithallial and epithallial cells, in order to detect possible ultrastructural changes. L. 15 corallioides shows the alternation of tiers of short-squared and long-ovoid/rectangular cells along the perithallus, forming a typical banding. The perithallial cell length decreases according to water depth and growth-rate, whereas diameter remains constant. Our observations confirm that both epithallial and perithallial cells show primary (PW) and secondary (SW) calcite walls. Rectangular tiles, with the long axis parallel to the cell membrane forming a multi-layered structure, characterize the PW. Flattened squared bricks characterize the SW with roundish outlines enveloping the cell and showing a zigzag pattern.
    [Show full text]
  • Marlin Marine Information Network Information on the Species and Habitats Around the Coasts and Sea of the British Isles
    MarLIN Marine Information Network Information on the species and habitats around the coasts and sea of the British Isles Maerl (Lithothamnion corallioides) MarLIN – Marine Life Information Network Marine Evidence–based Sensitivity Assessment (MarESA) Review Frances Perry & Angus Jackson 2017-03-30 A report from: The Marine Life Information Network, Marine Biological Association of the United Kingdom. Please note. This MarESA report is a dated version of the online review. Please refer to the website for the most up-to-date version [https://www.marlin.ac.uk/species/detail/1284]. All terms and the MarESA methodology are outlined on the website (https://www.marlin.ac.uk) This review can be cited as: Perry, F. & Jackson, A. 2017. Lithothamnion corallioides Maerl. In Tyler-Walters H. and Hiscock K. (eds) Marine Life Information Network: Biology and Sensitivity Key Information Reviews, [on-line]. Plymouth: Marine Biological Association of the United Kingdom. DOI https://dx.doi.org/10.17031/marlinsp.1284.2 The information (TEXT ONLY) provided by the Marine Life Information Network (MarLIN) is licensed under a Creative Commons Attribution-Non-Commercial-Share Alike 2.0 UK: England & Wales License. Note that images and other media featured on this page are each governed by their own terms and conditions and they may or may not be available for reuse. Permissions beyond the scope of this license are available here. Based on a work at www.marlin.ac.uk (page left blank) Date: 2017-03-30 Maerl (Lithothamnion corallioides) - Marine Life Information Network See online review for distribution map Lithothamnion corallioides. Collected from c. 10m depth.
    [Show full text]