Immunohistochemistry (Ihc), Special Stains (Ss), & Cish Requisition

Total Page:16

File Type:pdf, Size:1020Kb

Immunohistochemistry (Ihc), Special Stains (Ss), & Cish Requisition 2119 E. 93rd / L15 Cleveland, OH 44106 IMMUNOHISTOCHEMISTRY (IHC), 216.444.5755 or 800.628.6816 SPECIAL STAINS (SS), & CISH REQUISITION <<FORM_ID>> PATIENT INFORMATION (PLEASE PRINT IN BLACK INK) CLIENT INFORMATION ___________________________________________________________________________________________ Last Name First MI ___________________________________________________________________________________________ Address Birth Date Sex ¨ M ¨ F ___________________________________________________________________________________________ City SS # ___________________________________________________________________________________________ State Zip Home Phone ___________________________________________________________________________________________ Hospital/Physician Office atientP ID # Accession # ORDERING PHYSICIAN CONTACT MEDICAL NECESSITY NOTICE: When ordering tests for which Medicare reimbursement will be sought, physicians (or other individuals authorized by law to order tests) should only order tests that are medically necessary for the diagnosis or treatment of a patient, rather than for screening purposes. __________________________________________________________ Physician Name INSURANCE BILLING INFORMATION (PLEASE ATTACH CARD OR PRINT IN BLACK INK) BILL TO: ¨ Client/Institution ¨ Medicare ¨ Insurance (Complete insurance information below) ¨ Patient __________________________________________________________ PATIENT STATUS: ¨ Inpatient ¨ Outpatient ¨ Non-Hospital Patient Hospital discharge date: ______/______/______ Physician NPI# PRIMARY: ¨ Medicare ¨ Medicaid ¨ Other Ins. __________________________ ¨ Self ¨ Spouse ¨ Child __________________________________________________________ ___________________________________________________________________________________________ Physician Phone Subscriber Last Name First MI __________________________________________________________ ___________________________________________________________________________________________ Physician Email Beneficiary / Member # Group # ___________________________________________________________________________________________ Claims Address City State Zip ¨ Call Results to phone number: (________) _________________________ SECONDARY: ¨ No ¨ Yes (if Yes, please attach) ¨ Fax report to: (________) ____________________________________ DIAGNOSIS CODE (REQUIRED) ICD-10 Codes 1. ________________ 2. ________________ 3. ________________ SPECIMEN INFORMATION Other: __________________________________________ ¨ *Paraffin Blocks/Preferred Specimen type Collection Date: _____ / _____ / _____ Time: ____________ Tissue Block ID#(s): _____________________________ Breast Samples: CAP / ASCO Requirements Cell Block ID#(s): _______________________________ 10% neutral buffered formalin: ¨ Yes ¨ No ¨ Unknown Retrieved from Archive Date: _____ / _____ / _____ ¨ Slides (positive charged) HER2 Fixation >6 and <48 hours: ¨ Yes ¨ No ¨ Unknown Unstained Slide ID#(s): ___________________________ ER/PR Fixation >6 and <48 hours: ¨ Yes ¨ No ¨ Unknown Body Site: ________________________________________ Cold ischemic time ≤ 1 hour: ¨ Yes ¨ No ¨ Unknown TECHNICAL-ONLY TESTING SERVICES Cleveland Clinic Laboratories offers eIHC and eSS products to provide expedited services to pathologists who have ordered IHC and Special Stains Technical-Only services. Whole slide images of the glass slides can be made available online within 24 hours of the material receipt via our secure online respository at https://slidedepot.clevelandclinic.org. • Clients can follow a staining algorithm rather than initially requesting a large number of stains, some of which may ultimately be unnecessary. Additional stains can be ordered without re-submitting the paraffin block: 1. Clients submit paraffin block(s) for testing 2. Cleveland Clinic can create an additional five unstained slides which allows for up to five additional stains to be performed as long as two months after the initial request 3. Simply fill out a new requisition and fax it to 216.445.0724 4. Additional stains may be received the same day • Cases contain specimens and slide information, as well as controls that are conveniently at your fingertips. An email notification is sent when scanned slide images are available for viewing. ¨ eIHC/eSS – Check here to receive rapid access to whole slide images of IHC/Special stains* Email address: ________________________________________________________ *Email required to access images ¨ Check here if this case had eIHC/eSS performed at Cleveland Clinic within the last two months (Block shipment may not be required). Please contact ePathology Services at [email protected] to confirm. ______________________________________________________________________________________________________________________________________________ ______________________________________________________________________________________________________________________________________________ ¨ Technical Stain Only – Check here to have slides/blocks returned by UPS/FedEx ______________________________________________________________________________________________________________________________________________ ______________________________________________________________________________________________________________________________________________ GLOBAL TESTING SERVICES ¨ Stain with Cleveland Clinic Pathologist Interpretation ______________________________________________________________________________________________________________________________________________ <<FORM_ID>> IMMUNOHISTOCHEMISTRY AND CHROMOGENIC IN SITU (CISH) SPECIAL STAINS Antibody Clone Antibody Clone Antibody Clone GROUP I (Microorganisms) 14-3-3 Sigma 1433S01 Cytokeratin 7 OV-TL 12/30 Myogenin F5D Fite ACTH polyclonal Cytokeratin 19 A53-B/A2.26 NapsinA EP205 Gomori's Methenamine Silver (GMS) Actin, Muscle Specific HHF35 Cytokeratin 20 Ks20.8 Neurofilament 2F11 Gram Actin Smooth Muscle 1A4 Cytokeratin AE1/AE3 AE1/AE3 Neurofilament Cocktail 2F11+SMI32 Gridley Adenovirus M58+M73 Cytokeratin CAM 5.2 CAM 5.2 NK1/C3 NK1/C3 Steiner AFP polyclonal Cytokeratin KER903 34BE12 NKX3.1 polyclonal PAS/light green counterstain ALK D5F3 D2-40 D2-40 OCT-2 polyclonal Warthin-Starry ALK--1 ALK-1 Desmin D33 OCT 3/4 C10 Ziehl-Neelsen AFB AMACR (p504s) 13H4 DOG-1 K9 p16 E6H4 Amyloid A mc1 EBNA-2 PE2 p40 BC28 Amyloid Kappa polyclonal EBV CS1, CS2, CS3, CS4 p53 DO-7 GROUP II (All Other) Amyloid Lambda polyclonal E-Cadherin 4A2C7 p57 Ab-6 Alcian Blue, pH 2.5 Androgen Receptor AR441 EGFR 3C6 p63 4A4 Alcian Blue/Hyaluronidase Annexin A1 MRQ-3 EMA E29 p63/ERG DS 4A4+EPR3864 Alcian Blue/PAS Arginase-1 SP156 Enolase (NSE) BBS/NC/VI-H14 p501s 10E3 Aldehyde Fuchsin B72.3 (TAG-72) B72.3 EP4-Ber BER-EP4 PAP polyclonal Alizarin Red S b-Catenin 14 ER SP1 PAX-2 EP3251 ASD "Leder's Modificationi" BCL2 124 ERG EPR3864 PAX-5 24 Bielschowsky BCL2 EP36 EP36 FACTOR VIII polyclonal PAX-8 polyclonal Bile, Hall's Method BCL-6 PG-B6p FACTOR XIIIa AC-1A1 P-Component polyclonal Bodian BOB-1 polyclonal Fascin 55K-2 Perforin 5B10 Colloidal Iron C3d polyclonal FGFR3 B-9 PD1 NAT Colloidal Iron/Hyaluronidase C4d polyclonal FOXP1 C-19 PDGFR C20 Congo Red CA 125 M11 FOX-P3 236A/E7 PIN cocktail (p63/AMACR) 4A4+13H4 Copper (Rhodanine) CA IX polyclonal FSH polyclonal PLAP 8A9 Crystal Violet Calcitonin polyclonal Galectin PC4 PMS2 A16-4 Elastic (EVG) Calponin CALP Gastrin polyclonal PR 1.00E+02 Fontana-Masson Calretinin polyclonal GATA-3 L50-823 Prolactin polyclonal Giemsa (Mast Cell) CD1a O10 GCDFP-24 D6 PSA polyclonal Grimelius CD2 AB75 GCET1 RAM341 PSMA 3.00E+06 Iron Stain CD3 2VG6 GFAP polyclonal PTEN 6H2.1 Jones Methenamine Silver CD4 1F6 GH polyclonal PTH 105G7 Loxol Fast Blue CD5 4C7 Glucagon polyclonal RCC PN-15 Masson Trichrome CD7 LP15 Glutamine Synthetase GS-6 S-100 polyclonal Melanin Bleach CD8 1A5 Glypican-3 1G12 SALL4 6E3 Movat CD10 56C6 Granzyme B GrB-7 Serotonin polyclonal CD15 BRA4F1 HBcAg polyclonal SF-1 N1665 Mucicarmine CD19 BT51E HBsAg S1-210 SMMS1 SMMS1 Oil Red O CD20 L26 H-Caldesmon h-CD SOX10 polyclonal Orcein CD21 1F8 HCG polyclonal SOX-11 MRQ-58 PAS/amylase (PAS/D) CD22 FPC1 HER2 Neu 4B5 pSTAT 5 Y694/a Periodic Acid-Schiff (PAS) CD23 BU38 Hepatocyte OCH1ES STAT6 YE361 PTAH CD25 4C9 Herpes (HSVI&II) polyclonal Synaptophysin snp88 Reticulum CD30 BER-H2 HHV8 13B10 Syphilis polyclonal SAB CD31 JC/70A HMB-45 HMB-45 Tau/B-amyloid DS poly+6F/3D Tioflavin S CD33 PWS44 HPV KIH8 TCL-1A 1-21 Toluidine Blue CD34 QBEnd/10 IDH-1 H09 TCR BF1 8A3 Uric Acid CD35 Ber-MAC-DCR IgA polyclonal TCR-Gamma γ3.20 Von Kossa CD43 DF-T1 IgD polyclonal TdT polyclonal CD44 DF1485 IgG polyclonal TFE-3 MRQ-47 HISTOCHEMICAL STAINS (Enzyme) CD45 2B11,PD7/26 IgG4 HP6025 Thyroglobulin polyclonal CD45RO A6 IgM polyclonal TIA TIA-1 Acetylcholinesterase (ACE) CD56 1B6 Inhibin R1 Transthyretin 1F5 Acid Phosphatase CD57 NK-1 INI-1 25/BAF47 Tryptase, Mast Cell polyclonal Alkaline Phosphatase CD61 2F2 Insulin polyclonal TSH polyclonal ATP (4.6, 9.8) CD68 KP-1 Kappa polyclonal TTF-1 8G7G3/1 Cytochrome C CD68 PG-M1 Ki 67 30-9 Tyrosinase T311 Esterase CD71 10F11 Lambda polyclonal Ubiqutin polyclonal NADH CD79a JCB117 Langerin 12D6 Vimentin 3B4 Phosphofructokinase CD99 H036-1.1 LEF1 EPR2029Y VS38C VS38c Phosphorylase CD117 polyclonal LH C93 WT1 (6F-H2) 6F-H2 Succinic Dehydrogenase (SDH) CD123 7G3 LMO2 SP51 CD138 BC/B-B4 Mammaglobin 31A5 CD163 10D6 MDM2 IF2 CHROMOGENIC ISH PROBES CDX-2 CDX2-88 Melan A A103 CISH EBER CEA polyclonal MiTF C5+D5 CISH KAPPA Chromogranin A DAK-A3 MLH-1 G168-15 CISH LAMBDA Clusterin 41D MOC-31 MOC-31 CMV DDG9+CCH2 MSH-2 FE11 C-MYC Y69 MSH-6 BC/44 Cox-2 33 MUM-1 MUM1P CXCL-13 53610 Muramidase polyclonal Cyclin-D1 SP4 Myeloperoxidase polyclonal Cytokeratin 5/6 D5/16B4 MyoD1 5.8A.
Recommended publications
  • Corneal Endotheliitis with Cytomegalovirus Infection of Persisted
    Correspondence 1105 Sir, resulted in gradual decreases of KPs, but graft oedema Corneal endotheliitis with cytomegalovirus infection of persisted. Vision decreased to 20/2000. corneal stroma The patient underwent a second keratoplasty combined with cataract surgery in August 2007. Although involvement of cytomegalovirus (CMV) in The aqueous humour was tested for polymerase corneal endotheliitis was recently reported, the chain reaction to detect HSV, VZV, or CMV; a positive pathogenesis of this disease remains uncertain.1–8 Here, result being obtained only for CMV-DNA. Pathological we report a case of corneal endotheliitis with CMV examination demonstrated granular deposits in the infection in the corneal stroma. deep stroma, which was positive for CMV by immunohistochemistry (Figures 2a and b). The cells showed a typical ‘owl’s eye’ morphology (Figure 2c). Case We commenced systemic gancyclovir at 10 mg per day A 44-year-old man was referred for a gradual decrease in for 7 days, followed by topical 0.5% gancyclovir eye vision with a history of recurrent iritis with unknown drops six times a day. With the postoperative follow-up aetiology. The corrected visual acuity in his right eye was period of 20 months, the graft remained clear without 20/200. Slit lamp biomicroscopy revealed diffuse corneal KPs (Figure 1d). The patient has been treated with oedema with pigmented keratic precipitates (KPs) gancyclovir eye drops t.i.d. to date. His visual acuity without anterior chamber cellular reaction (Figure 1a). improved to 20/20, and endothelial density was The patient had undergone penetrating keratoplasty in 2300/mm2. Repeated PCR in aqueous humour for August 2006, and pathological examination showed non- CMV yielded a negative result in the 10th week.
    [Show full text]
  • IHC) Outreach Services
    Immunohistochemistry (IHC) Outreach Services Note t ype of fixative used if not neutral buffered f ormalin. Note t ype of tissue/specim en Unless specified otherwise, positive and negative controls react satisfactoril y. Antibody Classif ications: – IVD (In Vitro Diagnosis) – No disclaimer required. – ASR (Anal yte Specific Reagent) – m ust use a disclaimer on the report (See Belo w) ASR required disclaimer T his test was developed a nd its perform ance characteristics determ ined b y Marshf ield Labs. It has not been cleared or approved b y th e U.S. Food and Drug Adm inistration. T he FDA has determ ined that such clearance or approval is not necessar y. T his test is used f or clinical purposes. It should not be regarded as investigational or f or research. T his laborator y is certif ied under the Clinical Laborator y Im provem ent Am endm ents (CLIA) as qualified to perf orm high-complexity testing. Available Chrom ogen – All m ark ers have been validated with 3,3’-Diam inobenzidine T etrah ydrochloride (DAB) which results in a bro wn/black precipitate. DAB is the routine chrom ogen. In addition, som e m ark ers have also been validated using the Fast Red (RED), which results in a re d precipitate. If available wit h both chrom ogens and one is not selected, the def ault will be t he DAB chrom ogen. Antibod y Common Applications Staining Characteristics/Cla ssification If Other Than IVD Actin (muscle specific) Smooth, skeletal & cardiac muscle Cytoplasmic Actin (smooth muscle) Smooth muscle and myoepithelial cells Cytoplasmic and membrane Adrenocorticotropin (ACTH) Pituitary neoplasms Cytoplasmic Alpha-1-Fetoprotein (AFP) Hepatoma, germ cell tumors Cytoplasmic ALK Protein ALK1 positive lymphomas Cytoplasmic and/or nuclear Alpha-1-Antitrypsin Demonstrates A-1-AT in liver Cytoplasmic (A-1-AT) Bcl-2 Oncoprotein Follicular lymphoma and soft tissue Cytoplasmic tumors Bcl-6 Follicular lymphoma Nuclear Ber-EP4, Epithelial Antigen Adenocarcinoma vs.
    [Show full text]
  • Immunohistochemistry Immunohistochemistry
    !"#$%&'"()(*+$(,$-#)&.(-&/$ 0--1.("23'(4"#-23'5+$$ 65.&17$7#$)&$8(14"&57295#$:;<$=";$ >+(.<$85&.4#$ Immunohistochemistry Immunohistochemistry It’s all about chosing the adapted anbody(ies) Immunohistochemistry It’s all about chosing the adapted anbody(ies) for the selected task(s) AnAbodies • « Melanocyc » anbodies – S100 – MelanA – HMB45 – PNL2 – MiTF Specificity vs Sensivity – SOX10 – … • « Anomaly-specific » anbodies – BRAF V600E – NRAS Q61R – ALK – ROS1 – NTRK1 – MET – P16 – BAP1 – PDL1 – … • Other anbodies – D2-40 – CD68 HMB45 – … AnAbodies • « Melanocyc » anbodies – S100 – MelanA – HMB45 – PNL2 – MiTF – SOX10 – … • « Anomaly-specific » anbodies – BRAF V600E – NRAS Q61R – ALK – ROS1 – NTRK1 – MET – P16 – BAP1 – PDL1 – … • Other anbodies – D2-40 – CD68 NTRK1 – … AnAbodies • « Melanocyc » anbodies – S100 – MelanA – HMB45 – PNL2 – MiTF – SOX10 – … • « Anomaly-specific » anbodies – BRAF V600E – NRAS Q61R – ALK – ROS1 – NTRK1 – MET – P16 – BAP1 – PDL1 – … • Other anbodies (DD mainly) – D2-40 – CD68 – … Why perform IHC? • Confirm melanocyc lineage • Visualize the melanocytes • Benign vs Malignant • Molecular characterizaon A. Confirm melanocyc lineage • Unpigmented dermal or ulcerated tumor (No recognizable junconal melanocytes) • Unpigmented metastases • Desmoplasc melanoma A1. Confirm melanocyc lineage Unpigmented dermal or ulcerated tumor M, 65 Back 6Ha$[(.T5-$-#)&.(4+A4$)2.#&*#$ b.%2*-#.'#7$7#5-&)$(5$1)4#5&'#7$'1-(5$$ b.%2*-#.'#7$.#3'$(,$#%2'"#)2(27$4#))3$ A1. Confirm melanocyc lineage Unpigmented dermal or ulcerated tumor S100 Protein 6Ha$[(.T5-$-#)&.(4+A4$)2.#&*#$
    [Show full text]
  • APPLICATION NOTE Anti-Fluortag Antibodies Enable
    APPLICATION NOTE Anti-FluorTag Antibodies Enable Immunohistochemistry with Flow Cytometry Antibodies Yves Konigshofer, PhD, Alice Ku, Farol L. Tomson, PhD and Seth B. Harkins, PhD INTRODUCTION Figure 1 compares the sensitivity of fluorescence microscopy to immunohistochemistry. Sections of a mouse spleen were The KPL anti-FluorTag antibodies are a set of goat-derived first stained for dendritic cells using different concentrations polyclonal antibodies that can be used to detect Fluorescein of a FITC-labeled anti-CD11c antibody and then imaged with (FITC)-, Phycoerythrin (PE)-, Allophycocyanin (APC)- and a fluorescence microscope. Afterwards, HRP-labeled anti-FITC Peridinin Chlorophyll (PerCP)-labeled antibodies. They are and TrueBlue™ substrate were used to detect the FITC-labeled available unconjugated, as Horseradish Peroxidase (HRP) antibodies. At 93 ng/ml, background signals from autofluo- conjugates and as High Potency Alkaline Phosphatase rescence began to exceed the specific signal and at 20 ng/ml, (ReserveAP™) conjugates. FITC-, PE-, APC- and PerCP- very little FITC fluorescence was visible above background. labeled antibodies are used in flow cytometry. These fluores- However, the dendritic cells could still be detected with the cent tags are chosen due to their brightness and compatibility anti-FITC antibody in conjunction with TrueBlue peroxidase with lasers commonly found on flow cytometers. While flow stain. cytometry is very effective at determining what cells express a given protein at a given level, it provides no information about where those cells are located in relation to one another. For this, microscopy-based immunohistochemistry (IHC) tech- niques are frequently required. Ideally, this should not require the purchase and optimization of an entirely new set of pri- mary antibodies.
    [Show full text]
  • Immunohistochemistry in the Diagnosis of Cutaneous Infections
    Immunohistochemistry in the diagnosis of cutaneous infections Ana María Molina Ruiz Thesis for the fulfillment of the PhD degree in Medical Science UNIVERSIDAD AUTÓNOMA DE MADRID FACULTAD DE MEDICINA Department of Internal Medicine TESIS DOCTORAL Immunohistochemistry in the Diagnosis of Cutaneous Viral and Bacterial Infections AUTHOR: Ana María Molina Ruiz DIRECTOR: Luis Requena Caballero Department of Dermatology, Fundación Jiménez Díaz, Department of Internal Medicine, Universidad Autónoma, Madrid Memoria para optar al grado de Doctor en Medicina con Mención Internacional al Título. Tesis presentada como compendio de publicaciones. UNIVERSIDAD AUTÓNOMA DE MADRID FACULTAD DE MEDICINA Departamento de Medicina Interna D. Luis Requena Caballero, Catedrático de Dermatología del Departamento de Medicina de la Universidad Autónoma de Madrid. CERTIFICA: Que Dña. Ana María Molina Ruiz ha realizado bajo mi dirección el trabajo “Immunohistochemistry in the Diagnosis of Cutaneous Viral and Bacterial Infections“ que a mi juicio reúne las condiciones para optar al Grado de Doctor. Para que así conste, firmo el presente certificado en Madrid a 3 de septiembre del año dos mil catorce. Vº Bº Director de la Tesis Doctoral Profesor Luis Requena Caballero Catedrático de Dermatología Departamento de Medicina, Facultad de Medicina Universidad Autónoma de Madrid DERMATOPATHOLOGIE FRIEDRICHSHAFEN BODENSEE Dermatopathologische Gemeinschaftspraxis PD Dr. med. Heinz Kutzner, Dermatologe Postfach 16 46, 88006 Friedrichshafen Dr. med. Arno Rütten, Dermatologe Prof. Dr. med. Thomas Mentzel, Pathologe Dr. med. Markus Hantschke, Dermatologe Dr. med. Bruno Paredes, Dermatologe, Pathologe Dr. med. Leo Schärer, Dermatologe Postfach 16 46, 88006 Friedrichshafen Siemensstr. 6/1, 88048 Friedrichshafen May 28, 2014 To Whom it May Concern Gentlemen, I have read Dr.
    [Show full text]
  • Applications of Flow Cytometry and Immunohistochemistry to Diagnostic Hematopathology
    Review Article Applications of Flow Cytometry and Immunohistochemistry to Diagnostic Hematopathology Cherie H. Dunphy, MD c Objective.ÐDiagnostic hematopathology depends on selected studies to ensure reliable comparison of reported the applications of ¯ow cytometric immunophenotyping data. and immunohistochemical immunophenotyping combined Data Synthesis.ÐFlow cytometric immunophenotyping with the cytomorphology and histologic features of each offers the sensitive detection of antigens for which anti- case. Select cases may require additional ancillary cyto- bodies may not be available for paraf®n immunohisto- genetic and molecular studies for diagnosis. The purpose chemical immunophenotyping. However, paraf®n immu- of this review is to focus on the applications of ¯ow cy- nohistochemical immunophenotyping offers preservation tometric and immunohistochemical immunophenotyping of architecture and evaluation of expression of some pro- of paraf®n-embedded tissue to diagnostic hematopatholo- teins, which may not be available by ¯ow cytometric im- gy. Advantages and disadvantages of these techniques are munophenotyping. These techniques should be used as examined. complimentary tools in diagnostic hematopathology. Data Sources.ÐThe literature is extensively reviewed Conclusions.ÐThere are extensive applications of ¯ow (PubMed 1985±2003) with an emphasis on the most recent cytometric and immunohistochemical immunophenotyp- applications and those that are most useful clinically, both ing to diagnostic hematopathology. As cytogenetic and mo- diagnostically and prognostically. lecular ®ndings evolve in diagnostic hematopathology, Study Selection.ÐStudies were selected based on statis- there may be additional applications of ¯ow cytometric tically signi®cant results in large studies with reported ad- and immunohistochemical immunophenotyping to this equate clinical follow-up. ®eld of pathology. Data Extraction.ÐThe methodology was reviewed in the (Arch Pathol Lab Med.
    [Show full text]
  • Immunohistochemistry Stain Offerings
    immunohistochemistry stain offerings TRUSTED PATHOLOGISTS. INVALUABLE ANSWERS.™ MARCHMAY 20172021 www.aruplab.com/ap-ihcaruplab.com/ap-ihc InformationInformation in this brochurein this brochure is current is current as of as May of March 2021. 2017. All content All content is subject is subject to tochange. change. Please contactPlease ARUPcontact ClientARUP Services Client Services at 800-522-2787 at (800) 522-2787 with any with questions any questions or concerns.or concerns. ARUP LABORATORIES As a nonprofit, academic institution of the University of Utah and its Department We believe in of Pathology, ARUP believes in collaborating, sharing and contributing to laboratory science in ways that benefit our clients and their patients. collaborating, Our test menu is one of the broadest in the industry, encompassing more sharing and than 3,000 tests, including highly specialized and esoteric assays. We offer comprehensive testing in the areas of genetics, molecular oncology, pediatrics, contributing pain management, and more. to laboratory ARUP’s clients include many of the nation’s university teaching hospitals and children’s hospitals, as well as multihospital groups, major commercial science in ways laboratories, and group purchasing organizations. We believe that healthcare should be delivered as close to the patient as possible, which is why we support that provide our clients’ efforts to be the principal healthcare provider in the communities they serve by offering highly complex assays and accompanying consultative support. the best value Offering analytics, consulting, and decision support services, ARUP provides for the patient. clients with the utilization management tools necessary to prosper in this time of value-based care.
    [Show full text]
  • Principles of Immunohistochemistry Queen's Laboratory for Molecular
    Principles of Immunohistochemistry Queen’s Laboratory For Molecular Pathology Table of Contents POLYCLONAL VERSUS MONOCLONAL ANTIBODIES…....PAGE 3 DIRECT & INDIRECT ASSAYS………………………………….PAGE 4 LABELS…………………………………………………….……….PAGE 5 DETECTION…………………………………………………….…..PAGE 7 TISSUE PREP……………………………………………………….PAGE 9 BLOCKING ……………………………………………………….PAGE 10 RINSING….………………………………………………………..PAGE 10 CONTROLS…………………………………………….………….PAGE 11 2 Principles of Immunohistochemistry This pamphlet provides a general overview of the basics of IHC, giving some insights into the operating principles of antigen-antibody interactions and IHC protocols that take advantage of these interactions. Immune cells, or B cells, produce antibodies against targeted proteins. These antibodies then can recognize, and bind to, those proteins, called antigens. More specifically, they have an affinity for certain sites on those antigens, called epitopes. Antibodies are also called immunoglobulins, abbreviated Ig. There are different classes, or isotypes, of Ig molecules, designated with a third letter, such as IgG, or IgA. Different isotypes of immunoglobulins perform different functions, in different places in the body. IgG provides the majority of antibody based immunity against invading pathogens. Polyclonal and Monoclonal antibodies When the immune system detects the presence of a foreign body, such as a virus, many B cells will produce antibodies against it. The different B cells will target different sites on the antigen, producing a mix of antibodies, all against that virus, but specifically against many different epitopes on that antigen. This produces a polyclonal antiserum where “polyclonal” denotes the mixed population of Ig molecules. In contrast, a monoclonal antibody preparation contains a single antibody with specificity to one epitope on the antigen molecule. This is achieved by fusing B cells from the spleen of an immunized animal with immortal myeloma cells, and growing clones from the single parent cells on microtitre wells.
    [Show full text]
  • Newsletter 4
    ANATECH LTD INNOVATOR Special Stains Issue Special Stains Issue Hematoxylin and eosin (H & E) is the gold stan- dard for demonstration of tissue structure in anatomic pathology. However, by utilizing vari- ous dye solutions, special stains allow further visualization of major macromolecules (e.g., carbohydrates, proteins, minerals) in a rainbow of colors beyond the blue and pink hues of H & E staining. This makes special stains indispensable in the demonstration of tissue morphology and its components. While immunohistochemistry and molecular biology are truly advancing in 1 A B the diagnosis of diseases, the comparatively low cost of histochemical special stains makes them April 2010 vital to the pathology laboratory. Figure 1. Fatty liver metamorphosis. A) Iron, 20x; B) H&E, 40x. ANATECH LTD. has a growing family of really A new look special stains. We refer to them as really special because several of them are unique and were at some old favorites developed in response to a problem with the existing traditional stain, due to unavailability Iron or technical performance. By understanding the chemistry of dyes, ANATECH LTD. was able to Hemosiderin, an iron-storage complex, is normally respond to these problems and produce special present intracellularly in macrophages. However, stains that are chemically unique and/or offer during hemorrhaging, when red blood cells (RBC) are a technical improvement over the conventional released from the circulatory system, excess hemosid- stain. Knowing that the stained tissue’s appear- erin deposits will occur in the surrounding extracel- ance is critical, our really special stains yield lular spaces. This is seen grossly in the color change of similarly colored results as the traditional dyes.
    [Show full text]
  • Immunohistochemistry Principles, Uses and Methods INDEX
    Immunohistochemistry Principles, uses and methods INDEX Principles of Immunohistochemistry (IHC) ...................................3 Common Uses of IHC ....................................................................3 Fixation .............................................................................................4 Antigen Retrieval ..............................................................................4 IHC Methods ....................................................................................4 Antibodies .........................................................................................4 Indirect vs. Direct Detection Methods ...........................................5 Direct Detection Data .......................................................................6 The Complexity of Immunohistochemistry - Reagent Sourcing and Availability .................................................................7 IHC Reporters ...................................................................................8 Enzymes vs Fluorochromes ............................................................8 Counterstains ...................................................................................9 Expedeon products are sold for research purposes only, and our terms and conditions of sale include a limited use license to our Intellectual Property for internal research applications. Commercial use, such as use within manufacturing, re-sale to third parties, or incorporation into kits, requires a separate written agreement, conferring
    [Show full text]
  • Supporting Information
    Supporting Information Salazar et al. 10.1073/pnas.0804373105 SI Materials and Methods and postfixed for 24 h (4% paraformaldehyde and 0.1 M PBS). Chemicals and Antibodies. MPTP hydrochloride, 6-hydroxydopa- Alternatively, for TH cell counting in hemimesencephali, brains mine hydrobromide, Chelex 100, and D-amphetamine sulfate were postfixed for 48 h. Next, fixed brains were cryopreserved in were purchased from Sigma. Peptide N-glycosidase (PNGase) F 30% sucrose and cut on a freezing Microtome. Free-floating was purchased from New England Biolabs. DMT1 antibodies, sections were permeabilized, blocked for nonspecific binding previously developed and characterized (1), were used to rec- sites, and incubated with primary antibodies. Immunolabeling ognize the ϩIRE C-terminal region, the ϪIRE C-terminal was visualized using Alexa 488- and Cy3-conjugated secondary region, the 4th extracellular domain (Alpha Diagnostics), and antibodies (Invitrogen and Jackson ImmunoResearch, respec- the 3rd extracellular domain (Biosonda). In addition, the fol- tively) or HRP-conjugated secondary antibodies and DAB rev- lowing antibodies were used: rabbit anti-TH (Pel-Freez Biologi- elation (Vector Laboratories). cals), mouse anti-TH (Diasorin), rat anti-CD11b/Mac1 (Sero- tec), and mouse anti-actin (Sigma). Animals. Male C57BL/6J mice 12 weeks old were obtained from Janvier Breeding Center. Microcytic mice (MK/ReJ-mk/ϩ) were Tissue Preparation for Iron Measurement and Western Blot Analysis. obtained from Funmei Yang, University of Texas Health Sci- Within2hafterautopsy, the brains were dissected and blocks of ences Center, San Antonio, TX, and Mark Fleming, Harvard hemibrainstem were frozen in dry ice and stored at Ϫ80 °C. University School of Medicine, Boston, MA, and subsequently Serial 20-␮m-thick sections were cut from the frozen blocks at maintained as an inbred stock by breeding heterozygotes in a Ϫ12 °C by using a cryostat, thaw-mounted onto gelatin/ 129sv background.
    [Show full text]
  • Molecular Diagnostic Testing for Hematology and Oncology Indications Table of Contents Related Coverage Resources
    Medical Coverage Policy Effective Date.............................................. 8/15/2021 Next Review Date ..................................... 11/15/2021 Coverage Policy Number ................................... 0520 Molecular Diagnostic Testing for Hematology and Oncology Indications Table of Contents Related Coverage Resources Overview ....................................................................... 2 Genetics Coverage Policy ........................................................... 2 Genetic Testing Collateral File General Criteria for Somatic Pathogenic or Likely Pathogenic Variant Genetic Testing ........................... 2 Tumor Profile/Gene Expression Classifier Testing - ...................................................................... 3 Prostate Cancer Screening and Prognostic Tests ..... 6 Tumor Tissue-Based Molecular Assays for Prostate Cancer .......................................................... 6 Hematologic Cancer and Myeloproliferative and Myelodysplastic Disease ............................................ 7 Occult Neoplasms ....................................................... 9 Solid Tumor Cancers .................................................. 9 Other Tumor Profile Testing ....................................... 9 General Background .................................................... 9 General Criteria for Somatic Mutation Genetic Testing ........................................................................ 9 Tumor Profile/Gene Expression Classifier Testing .....................................................................
    [Show full text]