Supporting Information
Supporting Information Salazar et al. 10.1073/pnas.0804373105 SI Materials and Methods and postfixed for 24 h (4% paraformaldehyde and 0.1 M PBS). Chemicals and Antibodies. MPTP hydrochloride, 6-hydroxydopa- Alternatively, for TH cell counting in hemimesencephali, brains mine hydrobromide, Chelex 100, and D-amphetamine sulfate were postfixed for 48 h. Next, fixed brains were cryopreserved in were purchased from Sigma. Peptide N-glycosidase (PNGase) F 30% sucrose and cut on a freezing Microtome. Free-floating was purchased from New England Biolabs. DMT1 antibodies, sections were permeabilized, blocked for nonspecific binding previously developed and characterized (1), were used to rec- sites, and incubated with primary antibodies. Immunolabeling ognize the ϩIRE C-terminal region, the ϪIRE C-terminal was visualized using Alexa 488- and Cy3-conjugated secondary region, the 4th extracellular domain (Alpha Diagnostics), and antibodies (Invitrogen and Jackson ImmunoResearch, respec- the 3rd extracellular domain (Biosonda). In addition, the fol- tively) or HRP-conjugated secondary antibodies and DAB rev- lowing antibodies were used: rabbit anti-TH (Pel-Freez Biologi- elation (Vector Laboratories). cals), mouse anti-TH (Diasorin), rat anti-CD11b/Mac1 (Sero- tec), and mouse anti-actin (Sigma). Animals. Male C57BL/6J mice 12 weeks old were obtained from Janvier Breeding Center. Microcytic mice (MK/ReJ-mk/ϩ) were Tissue Preparation for Iron Measurement and Western Blot Analysis. obtained from Funmei Yang, University of Texas Health Sci- Within2hafterautopsy, the brains were dissected and blocks of ences Center, San Antonio, TX, and Mark Fleming, Harvard hemibrainstem were frozen in dry ice and stored at Ϫ80 °C. University School of Medicine, Boston, MA, and subsequently Serial 20-m-thick sections were cut from the frozen blocks at maintained as an inbred stock by breeding heterozygotes in a Ϫ12 °C by using a cryostat, thaw-mounted onto gelatin/ 129sv background.
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