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How Reversed-Phase Liquid Chromatography Works Mark R TO START MAKING A DIFFERENCE START HERE... SEE PAGE 9 January 2014 Volume 27 Number 1 www.chromatographyonline.com Expand Your Horizons A look at emerging sample preparation technologies LC TROUBLESHOOTING GC CONNECTIONS PITTCON PREVIEW What is the point of the column The origins of GC carrier gases A look to Chicago 2014 dead time? magentablackcyanyellow ES374251_LCE0114_CV1.pgs 01.09.2014 00:15 ADV G D NEWS The launch of our revolutionary Dried Blood Spot Autosampler is imminent! The Flow-Through Desorption (FTD™) technology inside enables automation of the entire workfl ow for DBS analysis by LC-MS/MS systems without any manual intervention. Come and get an exclusive preview at our booth at SLAS (#1417), MSACL (#32), Pittcon (#1839) or scan the QR-code for more information. CARE SAMPLE Spark Holland B.V. P. +31 591 631 700 Head Offi ce: P.O. box 388 F. +31 591 630 035 P. de Keyserstraat 8 7800 AJ Emmen E. [email protected] 7825 VE Emmen The Netherlands The Netherlands BETTER magentablackcyanyellow ES374203_LCE0114_CV2_FP.pgs 01.08.2014 23:34 ADV magentablackcyanyellow ES374850_LCE0114_003_FP.pgs 01.09.2014 23:54 ADV January | 2014 Volume 27 Number 1 COVER STORY 37 SAMPLE PREPARATION PERSPECTIVES Electrical Potential as a Driving Force in Sample Preparation Ronald E. Majors Entirely new sample preparation technologies continue to be introduced, mainly in the academic sector. Some of these technologies will undoubtedly stay in the academic laboratory. However, some new technologies may “cross the chasm” and eventually become a standard laboratory procedure. This instalment will examine some of those methods. Features 8 Determination of Sulphur-based Odourants in Commercially Available Natural Gas with Flow Modulated Comprehensive Two-Dimensional Gas Chromatography Taylor Hayward, Ronda Gras, and Jim Luong Pulsed-fl ow-modulated comprehensive two-dimensional gas chromatography with fl ame ionization detection was used to separate and identify all common sulphur compounds found in natural gas. 18 How Reversed-Phase Liquid Chromatography Works Mark R. Schure, Jake L. Rafferty, Ling Zhang, and J. Ilja Siepmann This tutorial on reversed-phase LC explains the role of solvent, chain conformation, solute position, and retention dynamics. 43 Pittcon 2014 Preview A look at what’s in store for chromatographers at Pittcon 2014, which will be held at McCormick Place South, Chicago, USA, from 2–6 March 2014. Columns 28 LC TROUBLESHOOTING Column Dead Time as a Diagnostic Tool John W. Dolan What good is that big, ugly peak at the beginning of the chromatogram? 33 GC CONNECTIONS The Origins of GC Carrier Gases: Putting a Genie in the Bottle John V. Hinshaw This month’s instalment tracks the progress of helium, hydrogen, and nitrogen carrier gases as they start their journey through a GC system. 50 THE ESSENTIALS Optimizing LC–MS and LC–MS–MS Methods Secondary parameters in the interface and mass analyzer can often Editorial Policy: have a major impact on sensitivity and reproducibility. This column All articles submitted to LC•GC Europe considers how and when to consider optimizing these parameters are subject to a peer-review process in association through a study of the working principles of LC–MS analysis. with the magazine’s Editorial Advisory Board. Cover: Departments Original materials courtesy: Westend61 45 Products 48 Events 4 LC•GC Europe January 2014 magentablackcyanyellow ES374255_LCE0114_004.pgs 01.09.2014 00:18 ADV *FAST LITTLE COLUMN Sure, it’s a funny looking column. Agilent AdvanceBio Protein A columns are specially designed for fast determination of MAb concentration in cell cultures. They use bio-monolith technology to produce fl ow-rate independent separations – no diffusion, no pores and no void volume – so you get your results in less time. The bio-monolith format enables fast and accurate MAb titer analysis over a wide concentration range with minimal plugging from cell debris. Don’t be put off by the funny shape; you’ll be delighted with what this fast little column can do for you. Download an application note for details and request a free trial at www.agilent.com/chem/advancebio magentablackcyanyellow ES374226_LCE0114_005_FP.pgs 01.08.2014 23:35 ADV Published by Editorial Advisory Board Kevin Altria Hans-Gerd Janssen Group Publisher Chief Executive Officer GlaxoSmithKline, Harlow, Essex, UK Van’t Hoff Institute for the Molecular Mike Tessalone Joe Loggia Daniel W. Armstrong Sciences, Amsterdam, The Netherlands [email protected] University of Texas, Arlington, Texas, USA Kiyokatsu Jinno Chief Executive Officer Michael P. Balogh School of Materials Sciences, Toyohasi Editorial Director Fashion Group, Executive Waters Corp., Milford, Massachusetts, USA University of Technology, Japan Laura Bush Vice-President Coral Barbas Huba Kalász Tom Florio Semmelweis University of Medicine, [email protected] Faculty of Pharmacy, University of San Pablo – CEU, Madrid, Spain Budapest, Hungary Executive Vice-President, Editor-in-Chief Brian A. 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