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Rapid Tests from Hardy Diagnostics which comply with the latest update of CLSI’s Standards M35-A2 for the Rapid ID of Bacteria and Yeast. Rapid Test Methods

Bacteria Presumptive ID n Additional Tests for Definitive ID n Organism Test Catalog No. Notes Brucella Typical morphology PDA negative (Cat. no. Z7331, K530, L21, Oxidase Z119 Z63) Forward to LRN reference laboratory Catalase Z62, Z76 Work in a safety cabinet; highly infectious MacConkey G35 Hemolysis A10 Urea L65 Indole Z65 Campylobacter Typical morphology Must be isolated from stool at 42°C on jejuni/coli Campy selective medium Motility Oxidase Z119 Catalase Z62, Z76 Hippurate hydrolysis DH4525, Z52 Indoxyl acetate Z111 Cardiobacterium Typical morphology Isolate must be from blood culture hominis Confirm with negative nitrate test Oxidase Z119 (Cat. no. K42, Z71, Z72, Z73, Z7071) Catalase Z62, Z76 Rapid Ornithine (Cat. no. K279) MacConkey G35 Hemolysis A10 Indole Z23 Eikenella Typical morphology corrodens Colonial morphology on Blood or chocolate A10, E14

in CO2 Oxidase Z119 Catalase Z62, Z76 MacConkey G35 Indole Z65 Hemolytic reaction A10 Distinct odor of bleach

Indoxyl Acetate Disks for Campylobater jejuni PYR Test Kit (Cat. no. Z75 and Z175) for Group CatScreen™ (Cat. no. Z110) for Moraxella (Cat. no. Z111), showing positive (left disk) and A Strep or Enterococcus. Readout in only two catarrhalis. Detects butyrase activity, readout in negative (right disk) reactions. minutes! only five minutes! Organism Test Catalog No. Notes Escherichia coli Oxidase Z119 Isolate must be growing as large colonies and not from gastrointestinal sites Indole Z65 Gram reaction or growth on gram-negative selective agar Hemolysis; or A10 Lactose, PYR Z75, Z175, Z104 MUG K9101 Francisella Typical morphology Work in a safety cabinet; highly infectious tularensis Forward to LRN reference laboratory Oxidase Z119 Catalase Z62, Z76 Growth characteristics on chocolate or around E14 Staphylococcus streak Beta lactamase Z51 Haemophilus Typical morphology Growth on BAP only around staph influenzae separates from Brucella and Francisella if Time for growth on Chocolate/BAP, E14, A10 necessary or MacConkey agar G35 ALA Z7081 Kingella kingae Typical morphology Found in sterile tissues and fluids Oxidase Z119 Catalase Z62, Z76 MacConkey G35 Hemolysis A10 Moraxella Typical morphology catarrhalis Oxidase Z119 Catalase Z62, Z76 Entire non-hemolytic colony moves intact on A10 BAP when pushed Butyrate; or Z110 Indoxyl acetate Z111 Neisseria Typical morphology Work in safety cabinet; highly infectious meningitidis Oxidase Z119

Colonial morphology on Chocolate in CO2 E14 g-glutamyl-aminopeptidase K9187

OxiStrip™ (Cat. no. Z93) An oxidase test, M.O.T. Disks (Cat. no. K9101) showing positive ALA Differentiation Disks for Haemophilus (Cat. paper strip with a stabilized sensitive reagent. MUG reaction (left) E. coli and negative MUG no. Z7081) showing positive (left disk) and See also our OxiDrops™ (Cat. no. Z119) for a reaction (right) Proteus mirabilis. Performs a negative (right disk) reactions under UV light. liquid ready-to-use reagent in dropper bottle. MUG, ONPG, and Indole test. Bacteria Presumptive ID n Additional Test for Definitive ID n Organism Test Catalog No. Notes Neisseria Typical morphology lactamica Oxidase Z119 Colonial morphology on BAP A10 b-galactosidase K9187 Neisseria Typical morphology gonorrhoeae Oxidase Z119 Catalase Z262 g-glutamyl-aminopeptidase; and K9187 b-galactosidase Proteus mirablis Spreading colony Ornithine (Cat. no. K279 ) or Maltose (Cat. no. Y85) Indole Z65 No other testing, if ampicillin susceptible Proteus vulgaris Spreading colony Indole Z65 Pseudomonas Oxidase Z119 Hemolysis on BA (Cat. no. A10) aeruginosa Indole Z65 Odor Staphylococcus Catalase Z62, Z76 Tube coagulase required if not hemolytic aureus Tube or slide coagulase (Rabbit plasma w/EDTA) Z202 or latex agglutination ST50 or ST200 Colonial morphology Staphylococcus Typical morphology May be slide coagulase positive or clumpy lugdunensis (Cat. no. ST50, ST200) Catalase Z62, Z76

Tube coagulase (Rabbit plasma w/EDTA) Z202

PYR and Z75, Z175 Polymixin B Rapid Ornithine K279 Enterococcus Typical morphology LAP positive if alpha hemolytic (Cat. no. spp. Catalase Z62, Z76 Z122) Hemolytic colony size on BAP A10 PYR Z75, Z175 Aerococcus Typical morphology PYR (Cat. no. Z122) viridans LAP (Cat. no. Z122) Catalase Z62, Z76 Hemolysis A10

Indole Spot Test (Cat. no. Z65) showing positive Rapid Ornithine media (Cat. no. K279) detects Catalase Test (Cat. no. Z62) showing a positive indole reaction. Filter paper was saturated with ornithine decarboxylase activity in as little catalase reaction. Growth was from a 24 hour Indole Spot reagent and E. coli colonies were as four hours. Reaction to the right shows a culture of Staphylococcus aureus. applied subsequently. positive results for Staphylococcus lugdunensis. StrepQuick™ (Cat. no. Z122) a rapid test for the StrepPRO™ Grouping Kit (Cat. no. PL030HD) Bile Spot Reagent (Cat. no. Z61) Streptococcus identification of gram-positive, catalase-negative a latex agglutination kit for beta hemolytic pneumoniae colony after incubation with Bile cocci. Helpful for ID of Streps, Aerococcus, streptococcus (A, B, C, D, F, and G). Spot Reagent. Reagent was dropped within Enterococcus, Gemella, Leuconostoc, and the black circle. Incubated aerobically for 30 Pediococcus. minutes at 35oC to dissolve colonies.

Organism Test Catalog No. Notes Listeria Typical morphology Isolate should be from either blood culture monocytogenes or CSF Catalase Z62, Z76 Hemolysis on BAP A10 Motility Hippurate DH4525 or Z52 Streptococcus Typical morphology In nonhemolytic, do not use hippurate agalactiae Catalase Z62, Z76 method (Group B) Hemolysis on BAP A10 Hippurate; or DH4525 or Z52 Rapid CAMP; or Z305 and Z306 Lancefield Group B by latex agglutination PL030HD Streptococcus Typical morphology May be Lancefield group A, C, F, or G by anginosus group Catalase Z62, Z76 latex agglutination (Cat. no. PL030HD) (“S. milleri”) Colony size and hemolysis on BAP Odor, or Lancefield group F by latex agglutination PL030HD Streptococcus Typical morphology Some strains have lost solubility to bile. pneumoniae Catalase Z62, Z76

Hemolysis on BAP

Bile solubility Z61 Streptococcus Typical morphology Carefully look at size and hemolysis since pyogenes enterococci can also be hemolytic (Group A) Catalase Z62, Z76 Hemolysis on BAP A10 Colony size PYR positive; or Z75, Z175 Lancefield group A by latex agglutination PL030HD Streptococcus Typical morphology If not in chains-Pediococcus is vancomycin viridans group resistant and Aerococcus urinae is a urinary Catalase Z62, Z76 pathogen in tetrads. Hemolysis A10 PYR Z75, Z175 LAP Z122 Bile solubility if alpha hemolytic Z61 Yeast

Organism Test Catalog No. Notes Candida albicans Budding yeast in smear Cannot separate from C. dubliniensis Feet in less than 48 hours Germ tube positive Z217 Candida glabrata Small yeast in smear with no hyphae Better growth on chocolate than BAP E14, A10 Growth better on EMB than blood: or G25

Rapid trehalose positive at 42o C Z205 Cryptococcus Spherical pleomorphic budding yeast with no hyphae Cannot differentiate from C. gattii neoformans Urea L65 Phenol Oxidase (Caffeic Acid) Z118, G213

Anaerobes - Gram-negative

Organism Test Catalog No. Bacteriodes fragilis group Colonial morphology on BAP/LKV A60 Cell morphology Colonial morphology on BBE agar G05 Bacteriodes ureolyticus Colonial morphology on BAP/LKV agar A60 Cell morphology BBE agar G05 Indole Z65 Bilophila wadsworthia Colonial morphology on BAP/LKV A60 Catalase Z262 Cell morphology BBE agar G05 Indole Z65 Fusobacterium nucleatum Colonial morphology on BAP/LKV A60 Cell morphology BBE agar G05 Indole Z65 Porphyromonas spp. Colonial morphology on BAP/LKV A60 Fluorescence A30 Cell morphology BBE agar G05 Indole Z65 Prevotella intermedia Colonial morphology on BAP/LKV A60 Fluorescence A30 Cell morphology BBE agar G05 Indole Z65 Prevotella spp. Indole Z65 Veillonella spp. Colonial morphology on BAP A30 Cell morphology BBE agar G05 Fluorescence Indole Z65 Anaerobes - Gram-positive

Organism Test Catalog No. C. difficile Colonial morphology on BAP A30 Odor Fluorescence Cell morphology Indole Z65 C. perfringens Colonial morphology on BAP A30 of beta-hemolysis on BAP Cell morphology Fluorescence C. septicum Colonial morphology on BAP A30 Cell morphology Indole Z65 C. sordellii Colonial morphology on BAP A30 Cell morphology C. tetani Colonial morphology on BAP A30 Cell morphology Peptostreptococcus spp. Colonial morphology on BAP A30 Cell morphology Propionibacterium acnes Colonial morphology on BAP A30 Catalase Z62 Cell morphology Indole Z65

Rapid MGP Media (Cat. no. Z225) to rule Germ Tube Cryo™ (Cat. no. Z217) a Nitrocef Matchbook (Cat. no. Z108) or out non-pathogenic Enterococcus spp. ready-to-use germ tube solution made Nitrocef Disk (Cat. no. Z7301) after a brief Results in five hours. up of stabilized animal serum, comes incubation, the positives will turn red/ pre-measured in vials. No mixing, orange as shown in the match-stick on the measuring, or aliquoting is required! For bottom. Negatives remain a buff color. Candida albicans. Detects resistance to beta lactam drugs.

Abbreviated Identification of Bacteria and Yeast; Approved Guideline -- Second Edition PRSRT STD U.S. POSTAGE PAID 1430 West McCoy Lane HARDY DIAGNOSTICS Santa Maria, CA 93455

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