Like Organisms 1. Slim Moulds 2. Stramenopila 3.True Fungi Eumycota

Total Page:16

File Type:pdf, Size:1020Kb

Like Organisms 1. Slim Moulds 2. Stramenopila 3.True Fungi Eumycota 6 th Lab Practical Mycology 2021 Fungi can be divided into three kingdoms: Kingdom of fungus- like organisms 1. Slim moulds 2. Stramenopila 3.True fungi a. Myxomycota a. Oomycota Eumycota b. Plasmodiophoromycota b. Hyphochytridiomycota a. Zygomycota c. Dictyosteliomycota c. Labyrinthulomycota b. Chytridiomycota d. Acrasiomycota c. Glomeromycota d. Ascomycota e. Basidiomycota f. Mitosporic Fungi Main traits of Phylum: Zygomycota 1. Reproduces asexually by the production of a non-motile spore called a sporangiospore . 2. Reproduces sexually by the production of a thick - walled zygospore . 3.They are the most ecologically diverse group of fungi. 4.Common name: Common molds, Pin mold, and Sugar mold. 5.They are molds that grow on meat, cheese and bread. 4. They are terrestrial organisms can be free-living or parasitic. 5.Cell walls comprise chitin and chitosan. Divided into two major classes Class: Zygomycetes and Trichomycetes. Class: Zygomycetes • Generally divided into 7 orders – Mucorales – Entomophthorales – Mortierellales – Kickxellales – Dimargaritales – Zoopagales – Endogonales Traits of class: Zygomycetes - Zygo (meaning zygote) + mycete (meaning fungus) = zygote fungus. - Produce non-septate and coenocytic hyphae. - Their cell walls contain chitin. - Flagellated spores are absent. - Reproduce asexually by sporangiospores within a special sac called the sporangium. - Sexual spores are called zygospore(s) contained within a zygosporangium. 1 6 th Lab Practical Mycology 2021 - Sex hormones are found to facilitate sexual reproduction among some zygomycetes are known to produce trisporic acids which are volatile and diffuse through the air. Class: Zygomycetes Order: Mucorales 1. They are mainly saprotrophs. 2. Many or one sporangiospore produced inside a sporangium. Order: Entomophthorales 1. They are mainly parasitic on arthropods. 2. They produce limited mycelium. 3. One sporangiospore produced inside sporangium. Order: Mucorales 1. Common genera include Mucor, and Rhizopus. 2. Form a septate hyphae but Some species form rhizoids. 3. Grow and invade quickly and easily on digestible substrates, such as those containing starches, sugars, and hemicelluloses. 4. Group lacks ability to spoil complex carbohydrates like cellulose. 5. Can act as parasites and/or cause diseases in plants (soft rot of fruits and vegetables), animals (predatory on nematodes and some insects and humans (zygomycosis - sometimes referred to as mucormycosis or phycomycosis). 6. Industrial uses of these fungi include production of metabolites (e.g., amylases, rennins, alcohol, and various organic acids - lactic acid, citric acid, succinic acid, oxalic acid). Moldy Bread and strawberries covered with Rhizopus mycelium and Mucor Sp 2 6 th Lab Practical Mycology 2021 Rhizoids Mycelia The fungal mass of hyphae, Sexual reproduction: known as the MYCELIUM The rhizoids meet penetrates the bread and underground and produces the fruiting bodies mating occurs between on top of the stalks hyphae of different molds Zygospores: thick-walled, usually hyaline in color, but zygosporangium wall often pigmented and ornamented. One zygospore per zygosporangium. Germination by formation of hyphae or sporangium. Sexually Produced Zygospores in Rhizopus Sp Zygospores in Rhizopus Sp Microscopic appearance of Rhizopus sp. (Black bread mold) 3 6 th Lab Practical Mycology 2021 Difference between Rhizopus sp. and . Mucor sp.: 1. Rhizoid can be produced in Rhizopus sp. but Hyphae produced in Mucor sp. Sporangium of Mucor sp. is bigger than its Rhizopus sp. 2. Density of mycelium of Rhizopus is much more than Mucor sp. mycelium. 3. Presence of stolon in Rhizopus sp. Rhizopus s Mucor sp. Microscopic appearance of Mucor sp. Asexual Reproduction - Large numbers of spores form in each sporangium. - When the sac opens at maturity, the spores are released. - If they land in favourable environments, they will germinate and form a new mycelium. Sexual Reproduction - Sexual reproduction occurs through conjugation. - Here, different strains of hyphae touch each other, and form a hardened zygospore, that can survive harsh conditions - Inside, the + and – nuclei will join to form a new diploid nuclei. - If conditions are favourable, the zygospore will germinate. - Upon meiosis, only one haploid nucleus remains, as the other three will degenerate. - The new haploid cell, will then give rise to a sporangiophore, which will go on to reproduce asexually. Plasmogamy Karyogamy 4 6 th Lab Practical Mycology 2021 Zygomycosis (obsolete name Phycomycosis and mucormycosis ) * In humans, disease is demonstrated in highly stressed or weakened individuals such as metabolic acidosis and immunosuppressant. * Most acute and rapidly fatal fungal disease. * Some patients die within one week after the onset of symptoms. * Death rate was about 90%, but now it is about 50 %. * Etiologic agents include: Rhizopus arrhizus and other species; Absidia sp., Mucor spp., and Mortierella sp. Order: Entomophthorales A. Entomophthora muscae, pathogen of houseflies. B. Massospora cicadina, pathogens of periodical cicadas. C. Basidiobolus ranarum, a commensal fungus of frogs and a mammal pathogen. D. Conidiobolus coronatus, a saprotrophic fungus of leaf litter and a mammal pathogen. E. Entomophaga maimaiga, a biocontrol agent of gypsy moths. Massopspora cicadina Entomophthora muscae Basidiobolus ranarum Conidiobolus coronatus Entomophaga maimaiga 5 6 th Lab Practical Mycology 2021 Life cycle of Rhizopus sp. 6 .
Recommended publications
  • Fungal Evolution: Major Ecological Adaptations and Evolutionary Transitions
    Biol. Rev. (2019), pp. 000–000. 1 doi: 10.1111/brv.12510 Fungal evolution: major ecological adaptations and evolutionary transitions Miguel A. Naranjo-Ortiz1 and Toni Gabaldon´ 1,2,3∗ 1Department of Genomics and Bioinformatics, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader 88, Barcelona 08003, Spain 2 Department of Experimental and Health Sciences, Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain 3ICREA, Pg. Lluís Companys 23, 08010 Barcelona, Spain ABSTRACT Fungi are a highly diverse group of heterotrophic eukaryotes characterized by the absence of phagotrophy and the presence of a chitinous cell wall. While unicellular fungi are far from rare, part of the evolutionary success of the group resides in their ability to grow indefinitely as a cylindrical multinucleated cell (hypha). Armed with these morphological traits and with an extremely high metabolical diversity, fungi have conquered numerous ecological niches and have shaped a whole world of interactions with other living organisms. Herein we survey the main evolutionary and ecological processes that have guided fungal diversity. We will first review the ecology and evolution of the zoosporic lineages and the process of terrestrialization, as one of the major evolutionary transitions in this kingdom. Several plausible scenarios have been proposed for fungal terrestralization and we here propose a new scenario, which considers icy environments as a transitory niche between water and emerged land. We then focus on exploring the main ecological relationships of Fungi with other organisms (other fungi, protozoans, animals and plants), as well as the origin of adaptations to certain specialized ecological niches within the group (lichens, black fungi and yeasts).
    [Show full text]
  • The Fungi of Slapton Ley National Nature Reserve and Environs
    THE FUNGI OF SLAPTON LEY NATIONAL NATURE RESERVE AND ENVIRONS APRIL 2019 Image © Visit South Devon ASCOMYCOTA Order Family Name Abrothallales Abrothallaceae Abrothallus microspermus CY (IMI 164972 p.p., 296950), DM (IMI 279667, 279668, 362458), N4 (IMI 251260), Wood (IMI 400386), on thalli of Parmelia caperata and P. perlata. Mainly as the anamorph <it Abrothallus parmeliarum C, CY (IMI 164972), DM (IMI 159809, 159865), F1 (IMI 159892), 2, G2, H, I1 (IMI 188770), J2, N4 (IMI 166730), SV, on thalli of Parmelia carporrhizans, P Abrothallus parmotrematis DM, on Parmelia perlata, 1990, D.L. Hawksworth (IMI 400397, as Vouauxiomyces sp.) Abrothallus suecicus DM (IMI 194098); on apothecia of Ramalina fustigiata with st. conid. Phoma ranalinae Nordin; rare. (L2) Abrothallus usneae (as A. parmeliarum p.p.; L2) Acarosporales Acarosporaceae Acarospora fuscata H, on siliceous slabs (L1); CH, 1996, T. Chester. Polysporina simplex CH, 1996, T. Chester. Sarcogyne regularis CH, 1996, T. Chester; N4, on concrete posts; very rare (L1). Trimmatothelopsis B (IMI 152818), on granite memorial (L1) [EXTINCT] smaragdula Acrospermales Acrospermaceae Acrospermum compressum DM (IMI 194111), I1, S (IMI 18286a), on dead Urtica stems (L2); CY, on Urtica dioica stem, 1995, JLT. Acrospermum graminum I1, on Phragmites debris, 1990, M. Marsden (K). Amphisphaeriales Amphisphaeriaceae Beltraniella pirozynskii D1 (IMI 362071a), on Quercus ilex. Ceratosporium fuscescens I1 (IMI 188771c); J1 (IMI 362085), on dead Ulex stems. (L2) Ceriophora palustris F2 (IMI 186857); on dead Carex puniculata leaves. (L2) Lepteutypa cupressi SV (IMI 184280); on dying Thuja leaves. (L2) Monographella cucumerina (IMI 362759), on Myriophyllum spicatum; DM (IMI 192452); isol. ex vole dung. (L2); (IMI 360147, 360148, 361543, 361544, 361546).
    [Show full text]
  • Subphyl. Nov., Based on Multi-Gene Genealogies
    ISSN (print) 0093-4666 © 2011. Mycotaxon, Ltd. ISSN (online) 2154-8889 MYCOTAXON Volume 115, pp. 353–363 January–March 2011 doi: 10.5248/115.353 Mortierellomycotina subphyl. nov., based on multi-gene genealogies K. Hoffmann1*, K. Voigt1 & P.M. Kirk2 1 Jena Microbial Resource Collection, Institute of Microbiology, University of Jena, Neugasse 25, 07743 Jena, Germany 2 CABI UK Centre, Bakeham Lane, Egham, Surrey TW20 9TY, United Kingdom *Correspondence to: Hoff[email protected] Abstract — TheMucoromycotina unifies two heterogenous orders of the sporangiferous, soil- inhabiting fungi. The Mucorales comprise saprobic, occasionally facultatively mycoparasitic, taxa bearing a columella, whereas the Mortierellales encompass mainly saprobic fungi lacking a columella. Multi-locus phylogenetic analyses based on eight nuclear genes encoding 18S and 28S rRNA, actin, alpha and beta tubulin, translation elongation factor 1alpha, and RNA polymerase II subunits 1 and 2 provide strong support for separation of the Mortierellales from the Mucoromycotina. The existence of a columella is shown to serve as a synapomorphic morphological trait unique to Mucorales, supporting the taxonomic separation of the acolumellate Mortierellales from the columellate Mucoromycotina. Furthermore, irregular hyphal septation and development of subbasally vesiculate sporangiophores bearing single terminal sporangia strongly correlate with the phylogenetic delimitation of Mortierellales, supporting a new subphylum, Mortierellomycotina. Key words — Zygomycetes, SSU rDNA, LSU rDNA, protein-coding genes, monophyly Introduction The type species ofMortierella Coem. 1863, M. polycephala Coem. 1863, was originally isolated from a parasitic interaction with a mushroom and named in honour of M. Du Mortier, the president of the Société de Botanique de Belgique (Coemans 1863). However, the common habit of mortierellalean species is as soil saprobes, enabling the fungi to grow on excrements, decaying plants, or (not infrequently) on decaying mushrooms and mucoralean fungi (Fischer 1892).
    [Show full text]
  • Examining New Phylogenetic Markers to Uncover The
    Persoonia 30, 2013: 106–125 www.ingentaconnect.com/content/nhn/pimj RESEARCH ARTICLE http://dx.doi.org/10.3767/003158513X666394 Examining new phylogenetic markers to uncover the evolutionary history of early-diverging fungi: comparing MCM7, TSR1 and rRNA genes for single- and multi-gene analyses of the Kickxellomycotina E.D. Tretter1, E.M. Johnson1, Y. Wang1, P. Kandel1, M.M. White1 Key words Abstract The recently recognised protein-coding genes MCM7 and TSR1 have shown significant promise for phylo genetic resolution within the Ascomycota and Basidiomycota, but have remained unexamined within other DNA replication licensing factor fungal groups (except for Mucorales). We designed and tested primers to amplify these genes across early-diverging Harpellales fungal clades, with emphasis on the Kickxellomycotina, zygomycetous fungi with characteristic flared septal walls Kickxellomycotina forming pores with lenticular plugs. Phylogenetic tree resolution and congruence with MCM7 and TSR1 were com- MCM7 pared against those inferred with nuclear small (SSU) and large subunit (LSU) rRNA genes. We also combined MS277 MCM7 and TSR1 data with the rDNA data to create 3- and 4-gene trees of the Kickxellomycotina that help to resolve MS456 evolutionary relationships among and within the core clades of this subphylum. Phylogenetic inference suggests ribosomal biogenesis protein that Barbatospora, Orphella, Ramicandelaber and Spiromyces may represent unique lineages. It is suggested that Trichomycetes these markers may be more broadly useful for phylogenetic studies among other groups of early-diverging fungi. TSR1 Zygomycota Article info Received: 27 June 2012; Accepted: 2 January 2013; Published: 20 March 2013. INTRODUCTION of Blastocladiomycota and Kickxellomycotina, as well as four species of Mucoromycotina have their genomes available The molecular revolution has transformed our understanding of (based on available online searches and the list at http://www.
    [Show full text]
  • Diversity of Entomopathogens Fungi: Which Groups Conquered
    bioRxiv preprint doi: https://doi.org/10.1101/003756; this version posted April 4, 2014. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Diversity of entomopathogens Fungi: Which groups conquered the insect body? João P. M. Araújoa & David P. Hughesb aDepartment of Biology, Penn State University, University Park, Pennsylvania, United States of America. bDepartment of Entomology and Department of Biology, Penn State University, University Park, Pennsylvania, United States of America. [email protected]; [email protected]; Abstract The entomopathogenic Fungi comprise a wide range of ecologically diverse species. This group of parasites can be found distributed among all fungal phyla and as well as among the ecologically similar but phylogenetically distinct Oomycetes or water molds, that belong to a different kingdom (Stramenopila). As a group, the entomopathogenic fungi and water molds parasitize a wide range of insect hosts from aquatic larvae in streams to adult insects of high canopy tropical forests. Their hosts are spread among 18 orders of insects, in all developmental stages such as: eggs, larvae, pupae, nymphs and adults exhibiting completely different ecologies. Such assortment of niches has resulted in these parasites evolving a considerable morphological diversity, resulting in enormous biodiversity, much of which remains unknown. Here we gather together a huge amount of records of these entomopathogens to comparing and describe both their morphologies and ecological traits. These findings highlight a wide range of adaptations that evolved following the evolutionary transition to infecting the most diverse and widespread animals on Earth, the insects.
    [Show full text]
  • S41467-021-25308-W.Pdf
    ARTICLE https://doi.org/10.1038/s41467-021-25308-w OPEN Phylogenomics of a new fungal phylum reveals multiple waves of reductive evolution across Holomycota ✉ ✉ Luis Javier Galindo 1 , Purificación López-García 1, Guifré Torruella1, Sergey Karpov2,3 & David Moreira 1 Compared to multicellular fungi and unicellular yeasts, unicellular fungi with free-living fla- gellated stages (zoospores) remain poorly known and their phylogenetic position is often 1234567890():,; unresolved. Recently, rRNA gene phylogenetic analyses of two atypical parasitic fungi with amoeboid zoospores and long kinetosomes, the sanchytrids Amoeboradix gromovi and San- chytrium tribonematis, showed that they formed a monophyletic group without close affinity with known fungal clades. Here, we sequence single-cell genomes for both species to assess their phylogenetic position and evolution. Phylogenomic analyses using different protein datasets and a comprehensive taxon sampling result in an almost fully-resolved fungal tree, with Chytridiomycota as sister to all other fungi, and sanchytrids forming a well-supported, fast-evolving clade sister to Blastocladiomycota. Comparative genomic analyses across fungi and their allies (Holomycota) reveal an atypically reduced metabolic repertoire for sanchy- trids. We infer three main independent flagellum losses from the distribution of over 60 flagellum-specific proteins across Holomycota. Based on sanchytrids’ phylogenetic position and unique traits, we propose the designation of a novel phylum, Sanchytriomycota. In addition, our results indicate that most of the hyphal morphogenesis gene repertoire of multicellular fungi had already evolved in early holomycotan lineages. 1 Ecologie Systématique Evolution, CNRS, Université Paris-Saclay, AgroParisTech, Orsay, France. 2 Zoological Institute, Russian Academy of Sciences, St. ✉ Petersburg, Russia. 3 St.
    [Show full text]
  • 454 Pyrosequencing Analysis of Fungal Assemblages from Geographically Distant, Disparate Soils Reveals Spatial Patterning and a Core Mycobiome
    Diversity 2013, 5, 73-98; doi:10.3390/d5010073 OPEN ACCESS diversity ISSN 1424-2818 www.mdpi.com/journal/diversity Article 454 Pyrosequencing Analysis of Fungal Assemblages from Geographically Distant, Disparate Soils Reveals Spatial Patterning and a Core Mycobiome Alberto Orgiazzi 1,2, Valeria Bianciotto 2, Paola Bonfante 1,2, Stefania Daghino 1, Stefano Ghignone 2, Alexandra Lazzari 1, Erica Lumini 2, Antonietta Mello 2, Chiara Napoli 1, Silvia Perotto 1, Alfredo Vizzini 1, Simonetta Bagella 3, Claude Murat 4 and Mariangela Girlanda 1,* 1 Department of Life Science and Systems Biology, University of Turin, Torino 10125, Italy; E-Mails: [email protected] (A.O.); [email protected] (P.B.); [email protected] (S.D.); [email protected] (A.L.); [email protected] (C.N.); [email protected] (S.P.); [email protected] (A.V.) 2 Plant Protection Institute (IPP)-Turin UOS, National Research Council (CNR), Torino 10125, Italy; E-Mails: [email protected] (V.B.); [email protected] (S.G.); [email protected] (E.L.); [email protected] (A.M.) 3 Department of Scienze della Natura e del Territorio, University of Sassari, Sassari 07100, Italy; Nucleo di Ricerca sulla Desertificazione—NRD, University of Sassari, Sassari 07100, Italy; E-Mail: [email protected] 4 INRA, UMR 1136, INRA-Nancy Université, Interactions Arbres/Microorganismes, Champenoux 54280, France; E-Mail: [email protected] * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +39-011-670-5968; Fax: +39-011-670-5962.
    [Show full text]
  • A Higher-Level Phylogenetic Classification of the Fungi
    mycological research 111 (2007) 509–547 available at www.sciencedirect.com journal homepage: www.elsevier.com/locate/mycres A higher-level phylogenetic classification of the Fungi David S. HIBBETTa,*, Manfred BINDERa, Joseph F. BISCHOFFb, Meredith BLACKWELLc, Paul F. CANNONd, Ove E. ERIKSSONe, Sabine HUHNDORFf, Timothy JAMESg, Paul M. KIRKd, Robert LU¨ CKINGf, H. THORSTEN LUMBSCHf, Franc¸ois LUTZONIg, P. Brandon MATHENYa, David J. MCLAUGHLINh, Martha J. POWELLi, Scott REDHEAD j, Conrad L. SCHOCHk, Joseph W. SPATAFORAk, Joost A. STALPERSl, Rytas VILGALYSg, M. Catherine AIMEm, Andre´ APTROOTn, Robert BAUERo, Dominik BEGEROWp, Gerald L. BENNYq, Lisa A. CASTLEBURYm, Pedro W. CROUSl, Yu-Cheng DAIr, Walter GAMSl, David M. GEISERs, Gareth W. GRIFFITHt,Ce´cile GUEIDANg, David L. HAWKSWORTHu, Geir HESTMARKv, Kentaro HOSAKAw, Richard A. HUMBERx, Kevin D. HYDEy, Joseph E. IRONSIDEt, Urmas KO˜ LJALGz, Cletus P. KURTZMANaa, Karl-Henrik LARSSONab, Robert LICHTWARDTac, Joyce LONGCOREad, Jolanta MIA˛ DLIKOWSKAg, Andrew MILLERae, Jean-Marc MONCALVOaf, Sharon MOZLEY-STANDRIDGEag, Franz OBERWINKLERo, Erast PARMASTOah, Vale´rie REEBg, Jack D. ROGERSai, Claude ROUXaj, Leif RYVARDENak, Jose´ Paulo SAMPAIOal, Arthur SCHU¨ ßLERam, Junta SUGIYAMAan, R. Greg THORNao, Leif TIBELLap, Wendy A. UNTEREINERaq, Christopher WALKERar, Zheng WANGa, Alex WEIRas, Michael WEISSo, Merlin M. WHITEat, Katarina WINKAe, Yi-Jian YAOau, Ning ZHANGav aBiology Department, Clark University, Worcester, MA 01610, USA bNational Library of Medicine, National Center for Biotechnology Information,
    [Show full text]
  • February 15 - Deadline: Inoculum 5 L(2) Prior to the 111 Latin American Mycological Congress in Caracas, March 15 - Deadlines: MSA Research Venezuela
    Newsletter of the Mycological Society of America -- In This Issue -- Caracas Asco Workshop .........................1-3 Thanks from Ian Ross .............................3-4 MSA Official Business Call for Papers ........................................ 4 From the President ...............................5-6 Committee Reports .............................. 6-7 2000 Award Announcements ............. 8- 1 1 MSA MEETING 2000 ................... 16- 17 Sustaining Members ............................. 29 Forms Change of Address .............................20 Gift Membership ...............................24 Endowment & Contributions ............30 Front row (1 to r) - Rick Weinstein, Rosario Medel, Karen Hatisen, Kris Peterson. Society Membership ..........................3 1 Marcela Solo; -Matias Cafaro, Sandy Salas, Paolo Porras, Josi Hernrindez, Mycology On-Line Directory ..................20 Mary Palm, Fernando Fernandez, Don Pfistet; Dick Hanlin. Emory Simmons Mycological News .................. 12-15, 18-20 Mycologist's Bookshelf ......................2 1-24 Ascos in Venezuela: Workshop memories Review: "lllustrated Genera of by Mary Palm Impeifect Fungi" Calendar of Events .............................. 27-28 Former MSA president DI:Palm (of the Beltsville USDA-APHIS Mycological Classifieds ..................... 25-27 lab) shares her and fellow August Asco workshoppers 'happy Positions, Goods & Services, Publica- memories of Enezuela before the devastatingjloods and landslides tions, Workshops, of December: - Important Dates - Late
    [Show full text]
  • Collecting and Recording Fungi
    British Mycological Society Recording Network Guidance Notes COLLECTING AND RECORDING FUNGI A revision of the Guide to Recording Fungi previously issued (1994) in the BMS Guides for the Amateur Mycologist series. Edited by Richard Iliffe June 2004 (updated August 2006) © British Mycological Society 2006 Table of contents Foreword 2 Introduction 3 Recording 4 Collecting fungi 4 Access to foray sites and the country code 5 Spore prints 6 Field books 7 Index cards 7 Computers 8 Foray Record Sheets 9 Literature for the identification of fungi 9 Help with identification 9 Drying specimens for a herbarium 10 Taxonomy and nomenclature 12 Recent changes in plant taxonomy 12 Recent changes in fungal taxonomy 13 Orders of fungi 14 Nomenclature 15 Synonymy 16 Morph 16 The spore stages of rust fungi 17 A brief history of fungus recording 19 The BMS Fungal Records Database (BMSFRD) 20 Field definitions 20 Entering records in BMSFRD format 22 Locality 22 Associated organism, substrate and ecosystem 22 Ecosystem descriptors 23 Recommended terms for the substrate field 23 Fungi on dung 24 Examples of database field entries 24 Doubtful identifications 25 MycoRec 25 Recording using other programs 25 Manuscript or typescript records 26 Sending records electronically 26 Saving and back-up 27 Viruses 28 Making data available - Intellectual property rights 28 APPENDICES 1 Other relevant publications 30 2 BMS foray record sheet 31 3 NCC ecosystem codes 32 4 Table of orders of fungi 34 5 Herbaria in UK and Europe 35 6 Help with identification 36 7 Useful contacts 39 8 List of Fungus Recording Groups 40 9 BMS Keys – list of contents 42 10 The BMS website 43 11 Copyright licence form 45 12 Guidelines for field mycologists: the practical interpretation of Section 21 of the Drugs Act 2005 46 1 Foreword In June 2000 the British Mycological Society Recording Network (BMSRN), as it is now known, held its Annual Group Leaders’ Meeting at Littledean, Gloucestershire.
    [Show full text]
  • Identification of Culture-Negative Fungi in Blood and Respiratory Samples
    IDENTIFICATION OF CULTURE-NEGATIVE FUNGI IN BLOOD AND RESPIRATORY SAMPLES Farida P. Sidiq A Dissertation Submitted to the Graduate College of Bowling Green State University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY May 2014 Committee: Scott O. Rogers, Advisor W. Robert Midden Graduate Faculty Representative George Bullerjahn Raymond Larsen Vipaporn Phuntumart © 2014 Farida P. Sidiq All Rights Reserved iii ABSTRACT Scott O. Rogers, Advisor Fungi were identified as early as the 1800’s as potential human pathogens, and have since been shown as being capable of causing disease in both immunocompetent and immunocompromised people. Clinical diagnosis of fungal infections has largely relied upon traditional microbiological culture techniques and examination of positive cultures and histopathological specimens utilizing microscopy. The first has been shown to be highly insensitive and prone to result in frequent false negatives. This is complicated by atypical phenotypes and organisms that are morphologically indistinguishable in tissues. Delays in diagnosis of fungal infections and inaccurate identification of infectious organisms contribute to increased morbidity and mortality in immunocompromised patients who exhibit increased vulnerability to opportunistic infection by normally nonpathogenic fungi. In this study we have retrospectively examined one-hundred culture negative whole blood samples and one-hundred culture negative respiratory samples obtained from the clinical microbiology lab at the University of Michigan Hospital in Ann Arbor, MI. Samples were obtained from randomized, heterogeneous patient populations collected between 2005 and 2006. Specimens were tested utilizing cetyltrimethylammonium bromide (CTAB) DNA extraction and polymerase chain reaction amplification of internal transcribed spacer (ITS) regions of ribosomal DNA utilizing panfungal ITS primers.
    [Show full text]
  • Introductory Mycology BI 432/532 Lecture 2: Overview of Fungi
    Introductory Mycology BI 432/532 Lecture 2: Overview of Fungi " Fungi are:! •# Microbes (mostly)! •# Eukaryotic, heterotrophic organisms that obtain nutrients by absorption and reproduce by spores. " "Extracellular enzymes act on complex substrates, low molecular weight breakdown products are absorbed through the fungal cell wall." " "Fungi live in their food." Nutrition" •# Heterotrophs (chemoheterotrophs)! •# Aerobes, facultative anaerobes (except Neocallimastix)" •# Absorptive nutrition" •# Secrete extracellular enzymes that act on complex substrates " •# Saprobes: decay dead organic matter" •# Parasites: biotroph, necrotroph " " Reproduce by spores! Reproduction, dissemination or survival structures" " A differentiated structure that may be specialized for dissemination, a resistant structure produced in response to adverse conditions, and/or produced during or as a result of a sexual or asexual reproductive process." " Spores may be one-celled or multicelled, colorless or pigmented (brown)" Fungal spores" Spores of some true fungi (chytrids), and fungus- like taxa (Oomycetes) are motile zoospores" Chytrid zoospores" have a single" posteriorly directed" flagellum" " Oomycetes" fungus-like organisms more closely related to plants than to true fungi" Oomycete zoospores have two flagella," one anteriorly directed and one posteriorly" directed" Spores of “higher fungi”—zygomycetes, ascomycetes, basidiomycetes— are non-motile" Spores of fungi may result from sexual (meiotic division) or asexual (mitotic division) processes" Major groups of
    [Show full text]