Cerium and Lanthanum Promote Floral Initiation and Reproductive Growth of Arabidopsis Thaliana

Total Page:16

File Type:pdf, Size:1020Kb

Cerium and Lanthanum Promote Floral Initiation and Reproductive Growth of Arabidopsis Thaliana Plant Science 159 (2000) 117–124 www.elsevier.com/locate/plantsci Cerium and lanthanum promote floral initiation and reproductive growth of Arabidopsis thaliana Ya-Wen He, Chiang-Shiong Loh * Department of Biological Sciences, National Uni6ersity of Singapore, 14 Science Dri6e 4, Singapore 117543, Singapore Received 1 December 1999; received in revised form 2 June 2000; accepted 5 July 2000 Abstract The effects of cerium and lanthanum on the vegetative growth, floral initiation and reproductive growth of Arabidopsis thaliana were studied. Addition of cerium nitrate (0.5–10 mM) or lanthanum nitrate (0.5–50 mM) to the culture medium significantly increased the lengths of primary roots, but had no significant effects on the number of rosette leaves produced per plant, plant heights and dry weights during the vegetative growth stage (17 days after seed germination). The percentage of plants bolted was significantly increased with the addition of 0.5–10.0 mM cerium nitrate or lanthanum nitrate. The combination of 0.5 mM cerium nitrate and 0.5 mM lanthanum nitrate was found to be most effective on the induction of floral initiation. The height, dry weight and average number of flower numbers of 35-day-old plants growing in media containing cerium nitrate or/and lanthanum nitrate (0.5–10.0 mM) were found to be significantly higher than those in the control medium. The endogenous levels of cytokinins (zeatin riboside, dihydrozeatin riboside and isopentenyl adenosine) and carbohydrates (sucrose, glucose and fructose) in leaf and root tissues of plants growing in the medium supplemented with 0.5 mM cerium nitrate and 0.5 mM lanthanum nitrate were not significantly different from those of plants in the control medium. Application of 0.5 mM cerium nitrate and 0.5 mM lanthanum nitrate enhanced the effects of 10−6 M IPA on root growth, plant height and flowering. The role of cerium and lanthanum in promoting floral initiation and reproductive growth and the possibility of developing non-hormonal flowering promoting agents are discussed. © 2000 Elsevier Science Ireland Ltd. All rights reserved. Keywords: Arabidopsis thaliana; Cerium; Lanthanum; Cytokinin; Flowering 1. Introduction REEs makes it imperative that more detailed in- formation on the effects of REEs on biological The rare earth elements (REEs) comprise a systems be available. group of 15 trivalent metallic elements with similar The effects of REEs, especially lanthanum, in chemical properties. They normally occur as basic different animal tissues have been studied exten- oxides and phosphate complexes in nature [1,2]. sively [1]. Most of the work carried out on REEs Since the introduction of ion exchange techniques, in animal systems was based on the use of REE the separation of the rare earth elements from ion as a substitute or antagonist for Ca2+ to their ores and from one another has become prac- monitor the movement of calcium and water, and tical and many new uses of REEs have been to investigate the role of calcium in muscle and developed [3]. The widespread industrial usage of nerve activity [4]. Abbre6iations: BA, benzyladenine; Ce, Cerium; DHZR, dihy- The results from limited studies on the effect of drozeatin riboside; IPA, isopentenyl adenosine; La, Lanthanum; MS REEs on plant growth are conflicting. Early re- medium Murashige and Skoog (1962) medium; Nd, Neodymium; ports indicated that the REE were inhibitory to REEs, rare earth elements; ZR, zeatin riboside. 3+ 3+ * Corresponding author. Tel.: +65-874-2916; fax: +65-779-5671. plant growth. For example, La and Nd were E-mail address: [email protected] (C.-S. Loh). found to inhibit elongation of oat coleoptile sec- 0168-9452/00/$ - see front matter © 2000 Elsevier Science Ireland Ltd. All rights reserved. PII: S0168-9452(00)00338-1 118 Y.-W. He, C.-S. Loh / Plant Science 159 (2000) 117–124 tions [5]. Colloidal lanthanum caused an almost drate (La(NO3)3·6H2O, Sigma) were dissolved in complete inhibition of cell division and root elon- Mili-Q water and sterilized by membrane filtra- gation in the root tips of barley plants [6]. La3+ tion (Millipore, 0.45 mm) and stored at room had been shown to inhibit root elongation of temperature in the dark. Stock solutions of wheat [7,8]. More recent reports, however, cerium nitrate and lanthanum nitrate were added demonstrated some positive effects of REEs on to the autoclaved basal medium prior to dispens- plant growth. Diatloff et al. [9] reported that ing into Magenta GA7® containers (Magenta corn root growth increased significantly with the Corp., USA). Seeds were germinated in the dark applications of cerium (0.63 mM) and lanthanum and 2-day-old seedlings were placed under 16 h (0.63 mM). Applications of lanthanum and photoperiod (54 mmol−1m−2s−1 provided by cerium were also reported to increase spike pro- Cool White fluorescent lamps) at 2592°C. duction in wheat [10]. In pot trials, applications of cerium sulphate (up to 100 mg/kg) enhanced 2.2. Growth measurements root and shoot growth of Phaseolus radiatus and Brassica pekinensis [11]. Lengths of primary roots were scored 10 days Results from field trials were also inconsistent. after seed germination. The number of leaves The increase in crop yield reported by workers produced per plant was scored 17 days after seed from China ranged between 8 and 50%, with the germination. Plant heights and dry weights were common response being of the order of 8–15% scored 17 and 35 days after seed germination, [12,13]. However, no response was found by respectively. Dry weights were taken by drying spraying and seed dressing of a summer fodder 100 plants in an oven (55°C) for 1 week. Floral crop (Brassica sp.) with REEs in a field trial initiation was recorded when the plant bolted carried out in Australia (cited in [4]). In view of with at least 1 cm long inflorescence stalk. this, it is essential to study and elucidate the effects of REE on essential stages of growth and 2.3. Extraction and determination of endogenous development of a model plant species such as cytokinins and carbohydrates Arabidopsis thaliana. In this report, we investi- gated the effects of cerium (Ce) and lanthanum Approximately 1 g fresh weight of tissues was (La) on vegetative and reproductive growth of A. homogenized in 4 ml of 80% ethanol followed by thaliana and correlated some of the responses to 1 h incubation at 4°C. After centrifugation at increased sensitivity of cell to plant growth regu- 1670×g for 3 min, the supernatant was trans- lators. ferred to another centrifuge tube. The tissues were re-extracted with 2 ml of 80% ethanol, and the supernatant was pooled together after cen- trifugation. The extracts were vacuum evaporated 2. Materials and methods at 4°C (Eppendorf Concentrator 5301) to 1/4 volume and then stored at −20°C after filter- 2.1. Plant materials, culture media and growth sterilization (Milipore, 0.2 mm). The analysis of conditions zeatin riboside (ZR), dihydrozeatin riboside (DHZR), and isopentenyl adenosine (IPA) were Seeds of A. thaliana L. Heynh cv. Columbia performed by immunoassay detection kits (Sigma (LEHLE SEEDS, USA) were surface sterilized Chemical Company) according to the protocols by soaking in 75% alcohol for 30 s and followed provided by the manufacturer. All hormonal lev- by 15% Clorox® for 15 min. The seeds were then els were expressed in terms of pmol per gram rinsed five times in sterilized water prior to cul- fresh weight (pmol/g.f.wt). The analysis of su- ture. The 1/4 strength Murashige and Skoog crose, glucose and fructose were performed by medium [14] was used for seed germination and assay kits (Sigma Chemical Company) according as basal medium. The pH of the medium was to the protocols provided by the manufacturer. adjusted to 5.8 before agar (Difco, 0.8%) was The levels of sucrose were expressed in term of added. All media were autoclaved for 20 min at mg per gram fresh weight (mg/g.f.wt). The levels 121°C. Cerium nitrate hexahydrate (Ce(NO3)3 of glucose and fructose were expressed in term of m m ·6H2O, Sigma) and lanthanum nitrate hexahy- g per g fresh weight ( g/g.f.wt). Y.-W. He, C.-S. Loh / Plant Science 159 (2000) 117–124 119 3. Results tions of cerium nitrate or lanthanum nitrate (0.5– 2.5 mM) significantly increased the percentages of 3.1. Effects on 6egetati6e growth plants bolted on days 20 and 23. For example, 69.3% of the plants growing in the medium with The vegetative and reproductive growth of A. 2.5 mM cerium nitrate bolted on day 23 whereas thaliana plant was separated. Prior to bolting, an only 39.4% bolted in basal medium. About 65.7% A. thaliana plant consisted of a rosette of small of the plants in the medium containing 0.5 mM leaves with a main hypocotyl. In the present lanthanum nitrate bolted on day 23 (Table 2). study, results showed that additions of 0.5–50 mM The percentages of plants bolted on day 27 were cerium or lanthanum had no significant effects on not significantly different between media with or the vegetative growth in terms of height and dry without cerium nitrate or lanthanum nitrate weight. Cerium and lanthanum also had no sig- (Table 2). nificant effect on the average number of rosette None of the plants growing in basal medium leaves 17 days after seed germination (data not bolted on day 19. Additions of certain combina- shown). Additions of higher concentrations of tions of cerium nitrate and lanthanum nitrate (e.g. cerium or lanthanum inhibited the vegetative 0.5+0.5 mM, 0.5+1.0 mM, 1.0+0.5 mM, 1.0+ growth (data not shown).
Recommended publications
  • Foreign-Trade Zone (FTZ)
    This document is scheduled to be published in the Federal Register on 04/14/2020 and available online at federalregister.gov/d/2020-07794, and on govinfo.gov BILLING CODE 3510-DS-P DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [B-21-2020] Foreign-Trade Zone (FTZ) 70 – Detroit, Michigan, Notification of Proposed Production Activity, Pacific Industrial Development Corporation (Zeolites, Specialty Alumina Products, Rare Earth Powders and Aqueous Solutions), Ann Arbor, Michigan Greater Detroit Foreign-Trade Zone, Inc., grantee of FTZ 70, submitted a notification of proposed production activity to the FTZ Board on behalf of Pacific Industrial Development Corporation (PIDC), located in Ann Arbor, Michigan. The notification conforming to the requirements of the regulations of the FTZ Board (15 CFR 400.22) was received on April 6, 2020. The PIDC facility is located within FTZ 70. The facility is used for the production of zeolites, specialty alumina products, rare earth powders and aqueous solutions for use in a variety of industries. Pursuant to 15 CFR 400.14(b), FTZ activity would be limited to the specific foreign-status materials and components and specific finished products described in the submitted notification (as described below) and subsequently authorized by the FTZ Board. Production under FTZ procedures could exempt PIDC from customs duty payments on the foreign-status components used in export production. On its domestic sales, for the foreign-status materials/components noted below, PIDC would be able to choose the duty rates
    [Show full text]
  • Federal Register/Vol. 85, No. 72/Tuesday, April 14, 2020/Notices
    20664 Federal Register / Vol. 85, No. 72 / Tuesday, April 14, 2020 / Notices ADDRESSES: Meeting to take place by call at 12:00 p.m. (EDT) on Thursday, reproduced at the Eastern Regional telephone. April 16, 2020. The purpose of the Office, as they become available, both FOR FURTHER INFORMATION CONTACT: meeting is to discuss possible work before and after the meetings. Persons Zakee Martin, (202)–376–7700, products in anticipation of the interested in the work of this advisory [email protected]. expiration of the current Advisory committee are advised to go to the Committee’s term, including a Commission’s website, www.usccr.gov, SUPPLEMENTARY INFORMATION: This Statement of Concern to the or to contact the Eastern Regional Office business meeting is open to the public Commission regarding incarceration at the above phone numbers, email, or by telephone only: 1–800–289–0449, issues. street address. Conference ID 209–3370. Computer assisted real-time DATES: Thursday, April 16, 2020; 12:00 Agenda transcription (CART) will be provided. p.m. (EDT). Public Call-In Information: Conference call-in number: 1–866–288– Thursday, April 16, 2020 at 12:00 p.m. The web link to access CART (in (EDT) English) on Friday, April 17, 2020, is 0540 and conference call 3166769. FOR FURTHER INFORMATION CONTACT: • Roll Call https://www.streamtext.net/ • player?event=USCCR. Please note that Evelyn Bohor at [email protected] or by Draft and Review Statement of phone at 202–376–7533. Concern Regarding CART is text-only translation that • occurs in real time during the meeting SUPPLEMENTARY INFORMATION: Interested Other Business • Open Comment and is not an exact transcript.
    [Show full text]
  • (IV) Oxidation of Organic Compounds: I. Benzyl and Related Alcohols, II
    Iowa State University Capstones, Theses and Retrospective Theses and Dissertations Dissertations 1968 Cerium (IV) oxidation of organic compounds: I. Benzyl and related alcohols, II. Cycloheptatriene Lewis Brewster Young Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/rtd Part of the Organic Chemistry Commons Recommended Citation Young, Lewis Brewster, "Cerium (IV) oxidation of organic compounds: I. Benzyl and related alcohols, II. Cycloheptatriene" (1968). Retrospective Theses and Dissertations. 3271. https://lib.dr.iastate.edu/rtd/3271 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Retrospective Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. This dissertation has been microfilmed exactly as received ® 8—10,491 YOUNG, Lewis Brewster, 1943- CERIUM(IV) OXIDATION OF ORGANIC COMPOUNDS. I. BENZYL AND RELATED ALCOHOLS, n. CYCLOHEPTATRIENE. Iowa State University, Ph.D., 1968 Chemistry, organic University Microfilms, Inc., Ann Arbor, Michigan CERIUM(IV) OXIDATION-OF ORGANIC COMPOUNDS. I, BENZYL AND RELATED ALCOHOLS. II. CYCLOHEPTATRIENE by Lewis Brewster Yoiang A Dissertation Submitted to the Graduate Faculty in Partial Fulfillment of The Requirements for the Degree of DOCTOR OF PHILOSOPHY Major Subject: Organic Chemistry Approved; Signature was redacted for privacy. Iil^harge of Major Signature was redacted for privacy. if Majdr Department Signature was redacted for privacy. DéJan of Grstdùate Collège Iowa State University Ames, Iowa 1968 ii TABLE OF CONTENTS Page INTRODUCTION 1 PART I.
    [Show full text]
  • John's Corner
    www.natureswayresources.com JOHN’S CORNER: MINERALS - The Elements and What They Do (Part 35) by John Ferguson 57) Lanthanum (La) Lanthanum is the first element of a group that we call the "Rare Earth" elements or "Lanthanides". If one looks at the Periodic table below, notice the two rows at the bottom where they are grouped together as they have almost identical chemical properties. However, they have very different magnetic properties. 1 www.natureswayresources.com Lanthanum is found in igneous rocks at 30 ppm, shale at 20 ppm, and very little in sandstone or limestone. Soils average around 30 ppm, and very little are found in fresh or seawater. However, marine plants can have 10 ppm. Most of the rare earth elements are not rare in nature but often occur together in various minerals and were hard to separate (hence the name "rare"). Lighter flints are alloys of iron (Fe), lanthanum (La), cerium (Ce), and small amounts of praseodymium (Pr) and neodymium (Nd). Rare earth elements when oxidized are very heat-resistant and glow brightly when hot; thus, they are used in lantern mantles heated by burning gas. Lanthanum's most common electrical or oxidation state is +3 (as are most of the other rare earths). Lanthanum is often found in igneous rocks and in phosphorites used to produce fertilizers. It is used to produce colored glass and electronic components. Sandy soils have the least of this element with loamy soils the most. Organic matter has a high capacity to bind this element to levels 10X that of surrounding soil.
    [Show full text]
  • G/LIC/N/2/PHL/1 8 November 2016 (16-6182) Page: 1/3 Committee on Import Licensing Original
    G/LIC/N/2/PHL/1 8 November 2016 (16-6182) Page: 1/3 Committee on Import Licensing Original: English AGREEMENT ON IMPORT LICENSING PROCEDURES NOTIFICATION UNDER ARTICLE 5.1-5.4 OF THE AGREEMENT1 PHILIPPINES The following notification, dated 3 November 2016 is being circulated at the request of the delegation of the Philippines. 1. (a) Title / number / date of the law or regulation establishing / changing the import licensing procedure: • Republic Act No. 10591 dated 29 May 2013 and its IRR dated December 2013 – An Act Providing for a Comprehensive Law on Firearms and Ammunition and Providing Penalties for Violations Thereof, otherwise known as the “Comprehensive Firearms and Ammunition Regulation Act”. • R.A. No. 9516 - (An Act Further Amending the Provisions of P. D. No. 1866, as Amended, Entitled Codifying the Laws on Illegal/Unlawful Possession, Manufacture, Dealing in, Acquisition or Disposition of Firearms, Ammunition or Explosives or Instruments Used in the Manufacture of Firearms, Ammunition or Explosives, and Imposing Stiffer Penalties for Certain Violations thereof and for Other Relevant Purposes) dated 22 December 2008. • Implementing Rules and Regulations on Controlled Chemicals pursuant to Section 4-C to 4-F of PD 1866 as Amended by RA No. 9516 dated June 9, 2016 • Presidential Decree No. 1866 dated June 29, 1983 – Codifying the Laws on Illegal/Unlawful Possession, Manufacture, Dealing In, Acquisition or Disposition of Firearms, Ammunition or Explosives, and Imposing Stiffer Penalties for Certain Violations Thereof and for Relevant Purposes. • Republic Act 8294 dated June 6, 1997 – An Act Further Amending the Provisions of Presidential Decree No. 1866, as Amended, entitled “Codifying the Laws on Illegal/Unlawful Possession, Manufacture, Dealing in, Acquisition or Disposition, of Firearms, ammunition or Explosives, and Imposing Stiffer Penalties for Certain Violations Thereof and for Relevant Purposes.
    [Show full text]
  • High Purity Nitrate Salts and Nitric Acid in Bulk Quantities
    High Purity Nitrate Salts and Nitric Acid in Bulk Quantities GFS provides a domestic supply chain of high purity nitrate salts and nitric acid in bulk quantities Whether you use high purity nitric acid directly in your process or you require bulk nitrate salts with low trace metal impurities, we have the experience, expertise and capacity to provide you with a secure, domestic supply chain. Nitrate Salts are a core focus at GFS Chemicals. We have been producing high purity Nitrate compounds for over 80 years. Many of the World’s largest companies rely on GFS for their critical raw material needs. How safe is your supply chain? Can you get what you need, when you need it? Our vertical integration in Nitric Acid distillation allows us to consistently supply a wide array of compounds in a variety of purity and moisture levels. With package sizes ranging from 100 gram bottles to truckload quantities, we can be your partner from R&D to full scale production so you don’t have to re-qualify material throughout your process life cycle. Whether it be an off the shelf ACS reagent, a custom nitrate etchant, or anything in between; GFS has the flexibility and expertise to produce what you need. See some of our core products below: Aluminum Nitrate (Item #644) Ammonium Nitrate (Item #646) Barium Nitrate (Item #858) Bismuth Nitrate (Item #864) Cadmium Nitrate (Item #871) Calcium Nitrate (Item #779) Cerium Nitrate (Item #30) Cerium (IV) Ammonium Nitrate (several grades available - Item #13, 768, 7683 & 2909) Cesium Nitrate (Item #887) Cobalt Nitrate
    [Show full text]
  • The Effects of Physicochemical Properties of Ceo2 Nanoparticles on Toxicity to Soil Denitrification Processes" (2013)
    Clemson University TigerPrints All Theses Theses 5-2013 The ffecE ts of Physicochemical Properties of CeO2 Nanoparticles on Toxicity to Soil Denitrification Processes Jessica Dahle Clemson University, [email protected] Follow this and additional works at: https://tigerprints.clemson.edu/all_theses Part of the Biogeochemistry Commons Recommended Citation Dahle, Jessica, "The Effects of Physicochemical Properties of CeO2 Nanoparticles on Toxicity to Soil Denitrification Processes" (2013). All Theses. 1579. https://tigerprints.clemson.edu/all_theses/1579 This Thesis is brought to you for free and open access by the Theses at TigerPrints. It has been accepted for inclusion in All Theses by an authorized administrator of TigerPrints. For more information, please contact [email protected]. THE EFFECTS OF PHYSICOCHEMICAL PROPERTIES OF CeO2 NANOPARTICLES ON TOXICITY TO SOIL DENITRIFICATION PROCESSES A Thesis Presented to the Graduate School of Clemson University In Partial Fulfillment of the Requirements for the Degree Master of Science Environmental Toxicology by Jessica Teague Dahle May 2013 Accepted by: Dr. Yuji Arai, Committee Chair Dr. Elizabeth Carraway Dr. Lisa Bain ABSTRACT Due to the rise of nanotechnology in industry, the concentrations of engineered metal oxide nanoparticles being released to the environment are increasing daily. Cerium-based compounds such as nanoparticulate ceria (NP CeO2) have received much attention in the last several years due to their popular applications (e.g., mischmetal, pharmaceuticals, nanotechnology) in industrial and commercial uses. Like many emerging pollutants, NP CeO2 is often released to the environment, where its toxicological effects are not well known. Though several environmental exposure pathways have been identified for CeO2 NPs, a major route of NP introduction to the environment is via biosolids application to agricultural lands.
    [Show full text]
  • Environmental Geochemistry of Cerium: Applications and Toxicology of Cerium Oxide Nanoparticles
    Int. J. Environ. Res. Public Health 2015, 12, 1253-1278; doi:10.3390/ijerph120201253 OPEN ACCESS International Journal of Environmental Research and Public Health ISSN 1660-4601 www.mdpi.com/journal/ijerph Review Environmental Geochemistry of Cerium: Applications and Toxicology of Cerium Oxide Nanoparticles Jessica T. Dahle 1 and Yuji Arai 2,* 1 School of Agricultural, Forest and Environmental Science, Clemson University, Clemson, SC 29634, USA; E-Mail: [email protected] 2 Department of Natural Resources and Environmental Sciences, University of Illinois at Urbana-Champaign Urbana, IL 61801, USA * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +(217)-244-3602. Academic Editor: Mónica Amorim Received: 22 September 2014 / Accepted: 15 January 2015 / Published: 23 January 2015 Abstract: Cerium is the most abundant of rare-earth metals found in the Earth’s crust. Several Ce-carbonate, -phosphate, -silicate, and -(hydr)oxide minerals have been historically mined and processed for pharmaceutical uses and industrial applications. Of all Ce minerals, cerium dioxide has received much attention in the global nanotechnology market due to their useful applications for catalysts, fuel cells, and fuel additives. A recent mass flow modeling study predicted that a major source of CeO2 nanoparticles from industrial processing plants (e.g., electronics and optics manufactures) is likely to reach the terrestrial environment such as landfills and soils. The environmental fate of CeO2 nanoparticles is highly dependent on its physcochemical properties in low temperature geochemical environment. Though there are needs in improving the analytical method in detecting/quantifying CeO2 nanoparticles in different environmental media, it is clear that aquatic and terrestrial organisms have been exposed to CeO2 NPs, potentially yielding in negative impact on human and ecosystem health.
    [Show full text]
  • I a Database of Produced Water Constituents with Ranking Of
    A Database of Produced Water Constituents with Ranking of Human Health Risk By Sydney M. Joffre B.S. Chemical Engineering, University of Colorado, 2018 B.S. Environmental Engineering, University of Colorado, 2019 A thesis submitted to the Faculty of the Graduate School of the University of Colorado in partial fulfillment of the requirement for the degree Master of Science Department of Civil, Environmental, and Architectural Engineering 2020 Committee Members: Cloelle Danforth Karl Linden James Rosenblum Joseph Ryan i Abstract: Sydney M. Joffre (Master of Science, Civil, Environmental, and Architectural Engineering) A Database of Produced Water Constituents with Ranking of Human Health Risk Thesis Directed by Professor Joseph N. Ryan Produced water is the largest waste stream of upstream oil and gas production in terms of volume. This study aims to address the implications of produced water reuse applications and inadvertent releases. We created a database of compounds identified in produced water from onshore oil and gas operations in North America and developed a prioritization scheme for those chemicals based on potential risk to human health. Through a comprehensive literature review, we found 179 studies that met our inclusion criteria. In total, there were 1,337 chemicals with a Chemical Abstract Service (CAS) number and 41 general water quality parameters (e.g., total dissolved solids, alkalinity) in produced water reported by the studies. We used the database to create a list of unique chemicals that had data available through the U.S. Environmental Protection Agency’s CompTox Dashboard and were in two or more individual samples at concentrations above the method detection limit.
    [Show full text]
  • SYNTHESIS, KINETIC CONTROL and PROPERTIES ENGINEERING of CERIUM OXIDE NANOPARTICLES for BIOMEDICAL APPLICATIONS Tetyana Yudina
    ADVERTIMENT. Lʼaccés als continguts dʼaquesta tesi queda condicionat a lʼacceptació de les condicions dʼús establertes per la següent llicència Creative Commons: http://cat.creativecommons.org/?page_id=184 ADVERTENCIA. El acceso a los contenidos de esta tesis queda condicionado a la aceptación de las condiciones de uso establecidas por la siguiente licencia Creative Commons: http://es.creativecommons.org/blog/licencias/ WARNING. The access to the contents of this doctoral thesis it is limited to the acceptance of the use conditions set by the following Creative Commons license: https://creativecommons.org/licenses/?lang=en DOCTORAL THESIS SYNTHESIS, KINETIC CONTROL AND PROPERTIES ENGINEERING OF CERIUM OXIDE NANOPARTICLES FOR BIOMEDICAL APPLICATIONS Tetyana Yudina Director: Prof. Dr. Victor Puntes Catalan Institute of Nanoscience and Nanotechnology (ICN2) Universitat Autonoma de Barcelona (UAB) Bellaterra 2016 Tesi Doctoral Programa de Doctorat en Bioquímica, Biología Molecular i Biomedicina “ Synthesis, kinetic control and properties engineering of cerium oxide nanoparticles for biomedical applications ” Memòria presentada per aspirar al Grau de Doctor per Tetyana Yudina Director i supervisor: Víctor F. Puntes Tutor: Jaume Farrés Vicén Institut Català de Nanociència i Nanotecnología (ICN2) Universitat Autònoma de Barcelona (UAB) Facultat de Biociènces Departament de Bioquímica i Biología Molecular Bellaterra, 2016 TABLE OF CONTENTS TABLE OF CONTENTS TABLE OF CONTENTS ...........................................................................................................
    [Show full text]
  • Cerium(Iv) Oxidation of Organic Compounds
    This dissertation has been microfilmed exactly as received ® 8—10,491 YOUNG, Lewis Brewster, 1943- CERIUM(IV) OXIDATION OF ORGANIC COMPOUNDS. I. BENZYL AND RELATED ALCOHOLS, n. CYCLOHEPTATRIENE. Iowa State University, Ph.D., 1968 Chemistry, organic University Microfilms, Inc., Ann Arbor, Michigan CERIUM(IV) OXIDATION-OF ORGANIC COMPOUNDS. I, BENZYL AND RELATED ALCOHOLS. II. CYCLOHEPTATRIENE by Lewis Brewster Yoiang A Dissertation Submitted to the Graduate Faculty in Partial Fulfillment of The Requirements for the Degree of DOCTOR OF PHILOSOPHY Major Subject: Organic Chemistry Approved; Signature was redacted for privacy. Iil^harge of Major Signature was redacted for privacy. if Majdr Department Signature was redacted for privacy. DéJan of Grstdùate Collège Iowa State University Ames, Iowa 1968 ii TABLE OF CONTENTS Page INTRODUCTION 1 PART I. SYNTHESIS OF ALDEHYDES AND KETONES FROM BENZYL AND RELATED ALCOHOLS 4 PART II. A SPECTROPHOTOMETRIC STUDY OF CERIUM(IV) - ALCOHOL COMPLEXES 8' INTRODUCTION 9 HISTORICAL 11 TREATMENT OF DATA 24 RESULTS AI^D DISCUSSION 27 EXPERIMENTAL 71 PART III. THE MECHANISM OF CERIUM(IV) OXIDATION OF BENZYL ALCOHOLS 81 INTRODUCTION 82 HISTORICAL 85 RESULTS 94 DISCUSSION 106 EXPERIMENTAL 142 PART IV. CERIUM(IV) OXIDATION OF CYCLO- HEPTATRIENE 146 INTRODUCTION 147 RESULTS AND DISCUSSION 151 EXPERIMENTAL 178 SUMMARY 207 LITERATURE CITED 209 ACKNOWLEDGMENTS ' 217 iii LIST OF TABIjES Page Table 1. Oxidation of benzyl and related alcohols to aldehydes or ketones by aqueous eerie ammonium nitrate Table 2. Oxidation of benzyl and related alcohols to aldehydes or ketones by eerie ammonium nitrate in 50% aqueous acetic acid Table 3. Summary of equilibrium constants for cerium(IV)-alcohol complexes reported in the literature 16 Table 4.
    [Show full text]
  • Ce OBTAINING and SEPARATION from LANTHANUM TARGET
    jo (<; H. Niewodniczartski PL9700852 INSTITUTE OF NUCLEAR PHYSICS Krakdw, Poland. RAPORT No1 7 1 1/C CARRIER-FREE 139Ce OBTAINING AND SEPARATION FROM LANTHANUM TARGET BEZNOSNIKOWY ,39Ce OTRZYMYWANIEIWYDZIELANIE Z TARCZY LANTANOWEJ E. Ochab and R. Misiak H. Niewodniczanski Institute of Nuclear Physics Radzikowskiego 152, 31-342 Cracow, Poland Krakow 1995 VOL 28 6 1 t WYDANO NAKiADEM INSTYTUTU FIZYKI JADROWEJ IM. HENRYKA NIEWODNICZANSKIEGO KRAK6W, UL. RADZIKOWSKIEGO 152 Kopif kserograficzng wykonano w IFJ Krakow Abstract The isotope 139Ce is widely used as a component of calibration multisources. In our laboratory preparation of high purity carrier-free 139Ce was worked out. The thick target yield ofl39Ce produced by bombardment of139La with 13 MeV deuterons was found to be 4,3 pCi/pAh. The product I39Ce was separated from macroquantities of lanthanum by extraction with diethyl ether (DEE) in thesystem DEE- HNOs The optimum conditions of 139Ce isolation were determined. Introduction The procedure of chemical separation of cerium from other lanthanides follows the technique suggested by Wylie1 - cerium oxidated to the +4 state is extracted with diethyl ether as the ceric nitrate complex. Extraction of ceric cerium was examined by several workers employing, for example, tributyl phosphate2 , di(2-ethyl hexyl)orthophpsphoric acid3 etc., and it has generally been accepted that the butyl phosphate extraction is preferable. But, as Wylie points out, while ethyl ether extracts less than 0,3 % of lanthanum from 5 M nitric acid, butyl phosphate extraxts as much as 4-7% of lanthanum from its nitrate solution. Other disadvantages of TBP are the necessity for reduction of cerium before back-extraction into water and the necessity for an additional treatment to overcome phosphate contamination of the product.
    [Show full text]