DNA Research, 2019, 26(1), 67–83 doi: 10.1093/dnares/dsy039 Advance Access Publication Date: 20 November 2018 Full Paper Full Paper Genome sequence of the non-conventional wine yeast Hanseniaspora guilliermondii UTAD222 unveils relevant traits of this species and of the Hanseniaspora genus in the context of wine fermentation Isabel Seixas1,2, Catarina Barbosa1,2, Arlete Mendes-Faia1,2, Ulrich Gu¨ ldener3, Roge´ rio Tenreiro2, Ana Mendes-Ferreira1,2*, and Nuno P. Mira4* 1WM&B—Laboratory of Wine Microbiology & Biotechnology, Department of Biology and Environment, University of Tra´s-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal, 2BioISI-Biosystems and Integrative Sciences Institute, Faculdade de Cieˆncias, Universidade de Lisboa Campo Grande, 1749-016 Lisbon, Portugal, 3Department of Bioinformatics, Wissenschaftszentrum Weihenstephan, Technische Universita¨t Mu¨nchen, Maximus von-Imhof- Forum 3, 85354 Freising, Germany, and 4Department of Bioengineering, iBB - Institute for Bioengineering and Biosciences, Instituto Superior Te´cnico, Universidade de Lisboa, Avenida Rovisco Pais, 1049-001 Lisbon, Portugal *To whom correspondence should be addressed. Tel. þ351218419181. Email:
[email protected] (N.P.M.); Tel. þ351 259 350 550. Email:
[email protected] (A.M.-F.) Edited by Dr. Katsumi Isono Received 23 May 2018; Editorial decision 13 October 2018; Accepted 16 October 2018 Abstract Hanseanispora species, including H. guilliermondii, are long known to be abundant in wine grape- musts and to play a critical role in vinification by modulating, among other aspects, the wine sensory profile. Despite this, the genetics and physiology of Hanseniaspora species remains poorly understood. The first genomic sequence of a H.