Diabetes Publish Ahead of Print, published online July 23, 2007 Cideb regulates diet-induced obesityˈliver steatosis and insulin sensitivity by controlling lipogenesis and fatty acid oxidation John Zhong Li1*, Jing Ye2, 3 *, Bofu Xue1, Jingzhong Qi2, Jing Zhang3, Zhihong Zhou4, Qing Li3, Zilong Wen4, and Peng Li1, 2# 1Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong; 2Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing,100084, China; 3Department of Pathology, Fourth Military Medical University, Xi’an, China; 4Institute of Molecular and Cell Biology, Singapore; *Those authors contribute equally to this work. # To whom correspondence should be addressed: Dr. Peng Li: Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China
[email protected] Received for publication 11 January 2007 and accepted in revised form 29 June 2007. Additional information for this article can be found in an online appendix at http://diabetes.diabetesjournals.org. Copyright American Diabetes Association, Inc., 2007 Abstract Our previous study suggests that Cidea, a member of Cide family proteins that share sequence homology with the DNA fragmentation factor and expressed at high levels in brown adipose tissue, plays an important role in the development of obesity. Cideb, another member of Cide family protein, is highly expressed in the liver. Objective˖We would like to understand the physiological role of Cideb in the regulation of energy expenditure and lipid metabolism. Research design and Method: We generated Cideb-null mice by homologues recombination and then fed both wild type and Cideb-null mice with high-fat diet (58% fat).