DOCSLIB.ORG

Development of Representative Species-Level Molecular Markers and Morphological Character Analysis of Leucothoid Amphipods (Crustacea: Amphipoda)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Development of Representative Species-Level Molecular Markers and Morphological Character Analysis of Leucothoid Amphipods (Crustacea: Amphipoda) The University of Southern Mississippi The Aquila Digital Community Dissertations Spring 5-2010 Development of Representative Species-Level Molecular Markers and Morphological Character Analysis of Leucothoid Amphipods (Crustacea: Amphipoda) Kristine Nicolle White University of Southern Mississippi Follow this and additional works at: https://aquila.usm.edu/dissertations Part of the Biology Commons, Marine Biology Commons, and the Molecular Genetics Commons Recommended Citation White, Kristine Nicolle, "Development of Representative Species-Level Molecular Markers and Morphological Character Analysis of Leucothoid Amphipods (Crustacea: Amphipoda)" (2010). Dissertations. 929. https://aquila.usm.edu/dissertations/929 This Dissertation is brought to you for free and open access by The Aquila Digital Community. It has been accepted for inclusion in Dissertations by an authorized administrator of The Aquila Digital Community. For more information, please contact [email protected]. The University of Southern Mississippi DEVELOPMENT OF REPRESENTATIVE SPECIES-LEVEL MOLECULAR MARKERS AND MORPHOLOGICAL CHARACTER ANALYSIS OF LEUCOTHOID AMPHIPODS (CRUSTACEA: AMPHIPODA) by Kristine Nicolle White Abstract of a Dissertation Submitted to the Graduate School of The University of Southern Mississippi in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy May 2010 ABSTRACT DEVELOPMENT OF REPRESENTATIVE SPECIES-LEVEL MOLECULAR MARKERS AND MORPHOLOGICAL CHARACTER ANALYSIS OF LEUCOTHOID AMPHIPODS (CRUSTACEA: AMPHIPODA) by Kristine Nicolle White May 2010 Leucothoid amphipods were investigated using morphology and molecular rDNA gene sequence fragments. The morphological diagnostic characters for traditional taxonomy have been clarified, a molecular marker for representative species has been developed, and one of the current anamorph-leucomorph connections has been confirmed with molecular sequence data. Ultimately this study has combined traditional morphological and modern molecular methods to clarify the taxonomy and to propose a preliminary phylogeny of the Leucothoidae. Analysis of 18S rDNA gene fragments from 13 species in two genera supported the current morphological species designations and the separation of the family into two clades. Combined analysis of 18S rDNA and COI mtDNA gene fragments revealed cryptic speciation in one species occurring in Belize and Florida. For the first time in a single work, diagnostic descriptions were presented for all extant species of the Leucothoidae. Fourteen new species of Leucothoe and one new species of Paranamixis were described from Lizard Island, Australia. Morphological cladistic analysis of 64 species of the “leucothoid clade” and 33 species of the “anamixid clade” supported the collapse of the genus Leucothoella and the classification of the genera Anamixis and Paranamixis as a single genus. The poor resolution of trees in these cladistic analyses suggests that rapid ancient speciation is likely a driving factor in the evolution of this family. ii COPYRIGHT BY KRISTINE NICOLLE WHITE 2010 ACKNOWLEDGEMENTS I thank my committee members, Dr. Richard W. Heard, Dr. Jeffery Lotz, Dr. Robin Overstreet, Dr. Trisha Spears, and Dr. James Darwin Thomas for their help in producing this dissertation. I thank Dr. Richard Heard for his tremendous support of my studies and for his relentless enthusiasm for natural history. I thank Dr. Jeffery Lotz for the use of his molecular laboratory, equipment, and supplies and for his input on my research and writing. I thank Dr. Robin Overstreet for filling in gaps with supplies, for sharing his immense knowledge of taxonomy and systematics, and his critical editing of this dissertation. I thank Dr. Trisha Spears for her incredible patience, big heart, and guidance in the molecular work for this dissertation. I thank my mentor, Dr. James Darwin Thomas, for all of his support, guidance, and friendship over the last 9 years; especially the hundreds of hours of conversation that made this dissertation possible. Special thanks go to my honorary committee member, Sara E. LeCroy. Sara has been a friend, colleague, and advisor to me over the last 4 years. My success here at The Gulf Coast Research Laboratory would not be possible without the support she has provided. I thank all of the people at The Gulf Coast Research Laboratory who have assisted with different aspects of this dissertation, especially the molecular work, and who have encouraged me not to give up when things got frustrating. These people include Allison Walker, David Drumm, Brent Thoma, Jana Thoma, Sarah Ballard, Jerry McClelland, Jana Rocker, Jean Jovonovich, Steve Curran, and Marie Mullen. I thank the Gordon Gunter Library staff for their hard work and assistance. Special thanks go to Cathy Schloss for locating a tremendous amount of ancient literature through interlibrary loans. I thank my family for their ongoing support over the many years I have been pursuing degrees. They have always believed in me and encouraged my quest for knowledge. Special thanks go to my husband, Nathan, who constantly reminds me that there is nothing left to do but smile, smile, smile. iii Funding for this and other related studies was provided by National Science Foundation awards (NSF-DEB) Partnerships for Enhancing Expertise in Taxonomy (PEET) (0529749) and (NSF-DBI) Biological Research Collection (BRC) (0749755); The Crustacean Society Fellowship in Graduate Studies, 2007; The University of Southern Mississippi Dissertation and Thesis Research Grant, 2008; Lerner-Gray Fund for Marine Research Grant, 2009; Lytle Coastal Sciences Scholarship, 2009; and Marine and Estuarine Graduate Student Association Student Travel Award, 2009. iv TABLE OF CONTENTS ACKNOWLEDGEMENTS ............................................................................................... iii LIST OF ILLUSTRATIONS ............................................................................................. vi LIST OF TABLES ............................................................................................................. ix CHAPTER I. INTRODUCTION TO THE LEUCOTHOIDAE ................................................... 1 Objectives .............................................................................................................. 1 Historical Background ........................................................................................... 2 Speciation ............................................................................................................ 13 Statement of Problem ........................................................................................... 14 Importance of Study ............................................................................................. 16 Taxonomic Background ....................................................................................... 17 Distribution .......................................................................................................... 22 II. TAXONOMY AND SYSTEMATICS OF THE LEUCOTHOIDAE .................. 30 Material Studied ................................................................................................... 30 Morphological Laboratory Techniques and Data Management .......................... 30 Systematics of the Leucothoidae ......................................................................... 31 III. MORPHOLOGICAL CHARACTER ANALYSIS AND A PRELIMINARY PHYLOGENY FOR THE LEUCOTHOIDAE ................................................. 295 Material Studied ................................................................................................. 295 Morphological Laboratory Techniques and Data Management ........................ 295 Molecular Laboratory Techniques ..................................................................... 296 Morphological Analyses .................................................................................... 298 Molecular Analyses ........................................................................................... 299 Morphological Results ....................................................................................... 302 Molecular Results .............................................................................................. 306 Discussion of Morphological Cladograms ......................................................... 315 Molecular Discussion ........................................................................................ 357 Conclusions ........................................................................................................ 368 APPENDIXES ................................................................................................................ 374 REFERENCES ............................................................................................................... 409 v LIST OF ILLUSTRATIONS Figure 2.1 Leucothoe adelphe, White and Thomas, 2009, holotype male, 1.6 mm, AM P71436 (QLD 1792), Three Sisters Bommie, Yonge Reef; paratype female, 1.9 mm, AM P71271 (QLD 1767), Horseshoe Reef, Lizard Island, Great Barrier Reef .................................................................................................................... 213 2.2 Leucothoe adelphe, White and Thomas, 2009, holotype male, 1.6 mm, AM P71436 (QLD 1792), Three Sisters Bommie, Yonge Reef; paratype female, 1.9 mm, AM P71271 (QLD 1767), Horseshoe Reef, Lizard Island, Great Barrier Reef ...................................................................................................................
Load more

Recommended publications
  • Bacteria Associated with Tunicate, Polycarpa Aurata, from Lease Sea, Maluku, Indonesia Exhibiting Anti-Multidrug Resistant Bacteria
    BIODIVERSITAS ISSN: 1412-033X Volume 20, Number 4, April 2019 E-ISSN: 2085-4722 Pages: 956-964 DOI: 10.13057/biodiv/d200404 Bacteria associated with tunicate, Polycarpa aurata, from Lease Sea, Maluku, Indonesia exhibiting anti-multidrug resistant bacteria DIAH AYUNINGRUM1,5, RHESI KRISTIANA1, AYUNDA AINUN NISA2, SEPTHY KUSUMA RADJASA2, SAKTI IMAM MUCHLISSIN2,4, OCKY KARNA RADJASA2,3, AGUS SABDONO2, AGUS TRIANTO2, 1Department of Coastal Resource Management, Faculty of Fisheries and Marine Science, Universitas Diponegoro. Jl. Prof. Soedharto SH, Semarang 50275, Central Java, Indonesia 2Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro. Jl. Prof. Soedarto, SH, Tembalang, Semarang 50275, Central Java, Indonesia. Tel.: +62-24-7474698, Fax.: +62-24-7474698, email: [email protected]. 3Directorate of Research and Community Services, Ministry of Research, Technology and Higher Education. D Building, Jl. Jenderal Sudirman Pintu Satu, Senayan, Jakarta Pusat 10279, Indonesia 4Tropical Marine Biotechnology Laboratory, Universitas Diponegoro Jl. Lingkar Utara Undip, Semarang 50275, Central Java, Indonesia 1Department of Aquatic Resource Management, Faculty of Fisheries and Marine Science, Universitas Diponegoro. Jl. Prof. Soedharto SH, Semarang 50275, Central Java, Indonesia Manuscript received: 30 January 2019. Revision accepted: 10 March 2019. Abstract. Ayuningrum D, Kristiana R, Nisa AA, Radjasa SK, Muchlissin SI, Radjasa OK, Sabdono A, Trianto A. 2019. Bacteria associated with tunicate, Polycarpa aurata, from Lease Sea, Maluku, Indonesia exhibiting anti-multidrug resistant bacteria. Biodiversitas 20: 956-964. Tunicate is a rich secondary metabolites producer with various biological activities whether as an original producer or produced by the associated microorganisms. In this study, a total of 11 tunicate specimens were identified as Polycarpa aurata with four color variations based on morphological characteristic and COI gene identification and BLAST analysis.
    [Show full text]
  • Sea Squirt Symbionts! Or What I Did on My Summer Vacation… Leah Blasiak 2011 Microbial Diversity Course
    Sea Squirt Symbionts! Or what I did on my summer vacation… Leah Blasiak 2011 Microbial Diversity Course Abstract Microbial symbionts of tunicates (sea squirts) have been recognized for their capacity to produce novel bioactive compounds. However, little is known about most tunicate-associated microbial communities, even in the embryology model organism Ciona intestinalis. In this project I explored 3 local tunicate species (Ciona intestinalis, Molgula manhattensis, and Didemnum vexillum) to identify potential symbiotic bacteria. Tunicate-specific bacterial communities were observed for all three species and their tissue specific location was determined by CARD-FISH. Introduction Tunicates and other marine invertebrates are prolific sources of novel natural products for drug discovery (reviewed in Blunt, 2010). Many of these compounds are biosynthesized by a microbial symbiont of the animal, rather than produced by the animal itself (Schmidt, 2010). For example, the anti-cancer drug patellamide, originally isolated from the colonial ascidian Lissoclinum patella, is now known to be produced by an obligate cyanobacterial symbiont, Prochloron didemni (Schmidt, 2005). Research on such microbial symbionts has focused on their potential for overcoming the “supply problem.” Chemical synthesis of natural products is often challenging and expensive, and isolation of sufficient quantities of drug for clinical trials from wild sources may be impossible or environmentally costly. Culture of the microbial symbiont or heterologous expression of the biosynthetic genes offers a relatively economical solution. Although the microbial origin of many tunicate compounds is now well established, relatively little is known about the extent of such symbiotic associations in tunicates and their biological function. Tunicates (or sea squirts) present an interesting system in which to study bacterial/eukaryotic symbiosis as they are deep-branching members of the Phylum Chordata (Passamaneck, 2005 and Buchsbaum, 1948).
    [Show full text]
  • Life-History Strategies of a Native Marine Invertebrate Increasingly Exposed to Urbanisation and Invasion
    Temporal Currency: Life-history strategies of a native marine invertebrate increasingly exposed to urbanisation and invasion A thesis submitted in partial fulfilment of the requirements for the degree of Master of Science in Zoology University of Canterbury New Zealand Jason Suwandy 2012 Contents List of Figures ......................................................................................................................................... iii List of Tables .......................................................................................................................................... vi Acknowledgements ............................................................................................................................... vii Abstract ................................................................................................................................................ viii CHAPTER ONE - General Introduction .................................................................................................... 1 1.1 Marine urbanisation and invasion ................................................................................................ 2 1.2 Successful invasion and establishment of populations ................................................................ 4 1.3 Ascidians ....................................................................................................................................... 7 1.4 Native ascidians as study organisms ............................................................................................
    [Show full text]
  • Natural Products Diversity of Marine Ascidians (Tunicates; Ascidiacea) and Successful Drugs in Clinical Development
    Nat. Prod. Bioprospect. DOI 10.1007/s13659-016-0115-5 REVIEW Natural Products Diversity of Marine Ascidians (Tunicates; Ascidiacea) and Successful Drugs in Clinical Development Satheesh Kumar Palanisamy . N. M. Rajendran . Angela Marino Received: 19 November 2016 / Accepted: 14 December 2016 Ó The Author(s) 2017. This article is published with open access at Springerlink.com Abstract This present study reviewed the chemical diversity of marine ascidians and their pharmacological applications, challenges and recent developments in marine drug discovery reported during 1994–2014, highlighting the structural activity of compounds produced by these specimens. Till date only 5% of living ascidian species were studied from\3000 species, this study represented from family didemnidae (32%), polyclinidae (22%), styelidae and polycitoridae (11–12%) exhibiting the highest number of promising MNPs. Close to 580 compound structures are here discussed in terms of their occurrence, structural type and reported biological activity. Anti-cancer drugs are the main area of interest in the screening of MNPs from ascidians (64%), followed by anti-malarial (6%) and remaining others. FDA approved ascidian compounds mechanism of action along with other compounds status of clinical trials (phase 1 to phase 3) are discussed here in. This review highlights recent developments in the area of natural products chemistry and biotechnological approaches are emphasized. Keywords Cancer Á Cytotoxicity Á Diversity Á Metabolites Á Pharmacology 1 Introduction from marine invertebrates, especially sponges, ascidians, bryozoans and molluscs in which some of them are The study of marine natural products (MNPs) is becoming approved by FDA and currently utilized in clinical trials ever more sophisticated and an increasingly collaborative [1].
    [Show full text]
  • The 17Th International Colloquium on Amphipoda
    Biodiversity Journal, 2017, 8 (2): 391–394 MONOGRAPH The 17th International Colloquium on Amphipoda Sabrina Lo Brutto1,2,*, Eugenia Schimmenti1 & Davide Iaciofano1 1Dept. STEBICEF, Section of Animal Biology, via Archirafi 18, Palermo, University of Palermo, Italy 2Museum of Zoology “Doderlein”, SIMUA, via Archirafi 16, University of Palermo, Italy *Corresponding author, email: [email protected] th th ABSTRACT The 17 International Colloquium on Amphipoda (17 ICA) has been organized by the University of Palermo (Sicily, Italy), and took place in Trapani, 4-7 September 2017. All the contributions have been published in the present monograph and include a wide range of topics. KEY WORDS International Colloquium on Amphipoda; ICA; Amphipoda. Received 30.04.2017; accepted 31.05.2017; printed 30.06.2017 Proceedings of the 17th International Colloquium on Amphipoda (17th ICA), September 4th-7th 2017, Trapani (Italy) The first International Colloquium on Amphi- Poland, Turkey, Norway, Brazil and Canada within poda was held in Verona in 1969, as a simple meet- the Scientific Committee: ing of specialists interested in the Systematics of Sabrina Lo Brutto (Coordinator) - University of Gammarus and Niphargus. Palermo, Italy Now, after 48 years, the Colloquium reached the Elvira De Matthaeis - University La Sapienza, 17th edition, held at the “Polo Territoriale della Italy Provincia di Trapani”, a site of the University of Felicita Scapini - University of Firenze, Italy Palermo, in Italy; and for the second time in Sicily Alberto Ugolini - University of Firenze, Italy (Lo Brutto et al., 2013). Maria Beatrice Scipione - Stazione Zoologica The Organizing and Scientific Committees were Anton Dohrn, Italy composed by people from different countries.
    [Show full text]
  • Former Fellows Biographical Index Part
    Former Fellows of The Royal Society of Edinburgh 1783 – 2002 Biographical Index Part Two ISBN 0 902198 84 X Published July 2006 © The Royal Society of Edinburgh 22-26 George Street, Edinburgh, EH2 2PQ BIOGRAPHICAL INDEX OF FORMER FELLOWS OF THE ROYAL SOCIETY OF EDINBURGH 1783 – 2002 PART II K-Z C D Waterston and A Macmillan Shearer This is a print-out of the biographical index of over 4000 former Fellows of the Royal Society of Edinburgh as held on the Society’s computer system in October 2005. It lists former Fellows from the foundation of the Society in 1783 to October 2002. Most are deceased Fellows up to and including the list given in the RSE Directory 2003 (Session 2002-3) but some former Fellows who left the Society by resignation or were removed from the roll are still living. HISTORY OF THE PROJECT Information on the Fellowship has been kept by the Society in many ways – unpublished sources include Council and Committee Minutes, Card Indices, and correspondence; published sources such as Transactions, Proceedings, Year Books, Billets, Candidates Lists, etc. All have been examined by the compilers, who have found the Minutes, particularly Committee Minutes, to be of variable quality, and it is to be regretted that the Society’s holdings of published billets and candidates lists are incomplete. The late Professor Neil Campbell prepared from these sources a loose-leaf list of some 1500 Ordinary Fellows elected during the Society’s first hundred years. He listed name and forenames, title where applicable and national honours, profession or discipline, position held, some information on membership of the other societies, dates of birth, election to the Society and death or resignation from the Society and reference to a printed biography.
    [Show full text]
  • A Bioturbation Classification of European Marine Infaunal
    A bioturbation classification of European marine infaunal invertebrates Ana M. Queiros 1, Silvana N. R. Birchenough2, Julie Bremner2, Jasmin A. Godbold3, Ruth E. Parker2, Alicia Romero-Ramirez4, Henning Reiss5,6, Martin Solan3, Paul J. Somerfield1, Carl Van Colen7, Gert Van Hoey8 & Stephen Widdicombe1 1Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, PL1 3DH, U.K. 2The Centre for Environment, Fisheries and Aquaculture Science, Pakefield Road, Lowestoft, NR33 OHT, U.K. 3Department of Ocean and Earth Science, National Oceanography Centre, University of Southampton, Waterfront Campus, European Way, Southampton SO14 3ZH, U.K. 4EPOC – UMR5805, Universite Bordeaux 1- CNRS, Station Marine d’Arcachon, 2 Rue du Professeur Jolyet, Arcachon 33120, France 5Faculty of Biosciences and Aquaculture, University of Nordland, Postboks 1490, Bodø 8049, Norway 6Department for Marine Research, Senckenberg Gesellschaft fu¨ r Naturforschung, Su¨ dstrand 40, Wilhelmshaven 26382, Germany 7Marine Biology Research Group, Ghent University, Krijgslaan 281/S8, Ghent 9000, Belgium 8Bio-Environmental Research Group, Institute for Agriculture and Fisheries Research (ILVO-Fisheries), Ankerstraat 1, Ostend 8400, Belgium Keywords Abstract Biodiversity, biogeochemical, ecosystem function, functional group, good Bioturbation, the biogenic modification of sediments through particle rework- environmental status, Marine Strategy ing and burrow ventilation, is a key mediator of many important geochemical Framework Directive, process, trait. processes in marine systems. In situ quantification of bioturbation can be achieved in a myriad of ways, requiring expert knowledge, technology, and Correspondence resources not always available, and not feasible in some settings. Where dedi- Ana M. Queiros, Plymouth Marine cated research programmes do not exist, a practical alternative is the adoption Laboratory, Prospect Place, The Hoe, Plymouth PL1 3DH, U.K.
    [Show full text]
  • First Molecular Barcoding and Record of the Indo-Pacific Punctuated Flatworm Maritigrella Fuscopunctata
    First molecular barcoding and record of the Indo-Pacific punctuated flatworm Maritigrella fuscopunctata (Newman & Cannon 2000), (Polycladida: Euryleptidae) from the Mediterranean Sea Adriana Vella, Noel Vella, Mathilde Maslin, Linda Bichlmaier To cite this version: Adriana Vella, Noel Vella, Mathilde Maslin, Linda Bichlmaier. First molecular barcoding and record of the Indo-Pacific punctuated flatworm Maritigrella fuscopunctata (Newman & Cannon 2000), (Poly- cladida: Euryleptidae) from the Mediterranean Sea. J. Black Sea/Mediterranean Environment, 2016, 22, pp.119 - 127. hal-02151343 HAL Id: hal-02151343 https://hal.archives-ouvertes.fr/hal-02151343 Submitted on 8 Jun 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. J. Black Sea/Mediterranean Environment Vol. 22, No. 2: 119-127 (2016) RESEARCH ARTICLE First molecular barcoding and record of the Indo-Pacific punctuated flatworm Maritigrella fuscopunctata (Newman & Cannon 2000), (Polycladida: Euryleptidae) from the Mediterranean Sea Adriana Vella*, Noel Vella, Mathilde Maslin, Linda Bichlmaier Conservation Biology Research Group, Department of Biology, University of Malta, Msida MSD2080, MALTA *Corresponding author: [email protected] Abstract A first record of the punctuated flatworm Maritigrella fuscopunctata (Newman & Cannon 2000) from Maltese waters in the Mediterranean Sea during marine research surveys in summer 2015 is reported in detail.
    [Show full text]
  • South Carolina Department of Natural Resources
    FOREWORD Abundant fish and wildlife, unbroken coastal vistas, miles of scenic rivers, swamps and mountains open to exploration, and well-tended forests and fields…these resources enhance the quality of life that makes South Carolina a place people want to call home. We know our state’s natural resources are a primary reason that individuals and businesses choose to locate here. They are drawn to the high quality natural resources that South Carolinians love and appreciate. The quality of our state’s natural resources is no accident. It is the result of hard work and sound stewardship on the part of many citizens and agencies. The 20th century brought many changes to South Carolina; some of these changes had devastating results to the land. However, people rose to the challenge of restoring our resources. Over the past several decades, deer, wood duck and wild turkey populations have been restored, striped bass populations have recovered, the bald eagle has returned and more than half a million acres of wildlife habitat has been conserved. We in South Carolina are particularly proud of our accomplishments as we prepare to celebrate, in 2006, the 100th anniversary of game and fish law enforcement and management by the state of South Carolina. Since its inception, the South Carolina Department of Natural Resources (SCDNR) has undergone several reorganizations and name changes; however, more has changed in this state than the department’s name. According to the US Census Bureau, the South Carolina’s population has almost doubled since 1950 and the majority of our citizens now live in urban areas.
    [Show full text]
  • Linnean 20-4 Web.P65
    THE LINNEAN Newsletter and Proceedings of THE LINNEAN SOCIETY OF LONDON Bur lington House , Piccadill y, London W1J 0BF VOLUME 20 • NUMBER 4 • OCTOBER 2004 THE LINNEAN SOCIETY OF LONDON Burlington House, Piccadilly, London W1J 0BF Tel. (+44) (0)20 7434 4479; Fax: (+44) (0)20 7287 9364 e-mail: [email protected]; internet: www.linnean.org President Secretaries Council Professor G McG Reid BOTANICAL The Officers and Dr J R Edmondson Dr L Allcock Vice-Presidents Prof J R Barnett Dr R M Bateman ZOOLOGICAL Prof S Blackmore Professor D F Cutler Dr V R Southgate Prof J Browne Dr J M Edmonds Dr J S Churchfield Dr V R Southgate EDITORIAL Dr J C David Professor D F Cutler Dr A Farjon Treasurer Dr M F Fay Professor G Ll Lucas OBE COLLECTIONS Dr P Kenrick Mrs S Gove Dr K N Maybury Executive Secretary Dr A D Rogers Mr Adrian Thomas OBE Librarian & Archivist Dr B R Rosen Miss Gina Douglas Dr D A Simpson Office/Facilities Manager Dr R A Sweeting Mr Dominic Clark Assistant Librarian Ms Cathy Broad Library Assistant Finance (on secondment to NHM) Mr Matthew Derrick Mr Priya Nithianandan Catalogue Coordinator Conservator Ms Lynn Crothall Ms Janet Ashdown THE LINNEAN Newsletter and Proceedings of the Linnean Society of London Edited by B. G. Gardiner Editorial .............................................................................................................. 1 Society News ............................................................................................................ 2 Library .............................................................................................................
    [Show full text]
  • A Taxonomic Review of the Leucothoidae (Crustacea: Amphipoda)
    Zootaxa 3078: 1–113 (2011) ISSN 1175-5326 (print edition) www.mapress.com/zootaxa/ Monograph ZOOTAXA Copyright © 2011 · Magnolia Press ISSN 1175-5334 (online edition) ZOOTAXA 3078 A taxonomic review of the Leucothoidae (Crustacea: Amphipoda) KRISTINE N. WHITE Rising Star Program, Trans-disciplinary Organization for Subtropical Island Studies (TRO-SIS) University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa JAPAN 903–0213. E-mail: [email protected] Magnolia Press Auckland, New Zealand Accepted by J. Lowry: 26 Aug. 2011; published: 28 Oct. 2011 KRISTINE N. WHITE A taxonomic review of the Leucothoidae (Crustacea: Amphipoda) (Zootaxa 3078) 113 pp.; 30 cm. 28 Oct. 2011 ISBN 978-1-86977-791-3 (paperback) ISBN 978-1-86977-792-0 (Online edition) FIRST PUBLISHED IN 2011 BY Magnolia Press P.O. Box 41-383 Auckland 1346 New Zealand e-mail: [email protected] http://www.mapress.com/zootaxa/ © 2011 Magnolia Press All rights reserved. No part of this publication may be reproduced, stored, transmitted or disseminated, in any form, or by any means, without prior written permission from the publisher, to whom all requests to reproduce copyright material should be directed in writing. This authorization does not extend to any other kind of copying, by any means, in any form, and for any purpose other than private research use. ISSN 1175-5326 (Print edition) ISSN 1175-5334 (Online edition) 2 · Zootaxa 3078 © 2011 Magnolia Press WHITE Table of contents Abstract . 5 Introduction . 6 Materials and methods. 12 Revised systematics of the Leucothoidae . 12 Leucothoidae Dana, 1852 . 13 Key to genera of Leucothoidae . 13 Anamixid clade .
    [Show full text]
  • New Cytotoxic Natural Products from Marine Invertebrates
    New cytotoxic natural products from marine invertebrates (Neue zytotoxische Naturstoffe aus marinen Invertebraten) Inaugural-Dissertation zur Erlangung des Doktorgrades der Mathematisch-Naturwissenschaftlichen Fakultät der Heinrich-Heine-Universität Düsseldorf vorgelegt von Cong-Dat Pham Aus Tönisvorst, Bundesrepublik Deutschland Düsseldorf, 03.02.2014 Aus dem Institut für Pharmazeutische Biologie und Biotechnologie der Heinrich-Heine Universität Düsseldorf Gedruckt mit der Genehmigung der Mathematisch-Naturwissenschaftlichen Fakultät der Heinrich-Heine-Universität Düsseldorf Gedruckt mit der Unterstützung des BMBF Referent: Prof. Dr. Peter Proksch Koreferent: Prof. Dr. Matthias U. Kassack Tag der mündlichen Prüfung: _______________ „Ich habe keine besondere Begabung, sondern bin nur leidenschaftlich neugierig.“ Albert Einstein CONTENTS 1. ABSTRACT 6 2. ZUSAMMENFASSUNG 9 3. INTRODUCTION 12 3.1 Significance of the study 12 3.2 Marine invertebrates 12 3.2.1 Sponges 13 3.2.2 Ascidians 15 3.2.3 Microbial communities in marine invertebrates 17 3.3 Marine natural products and their biological activities 18 3.3.1 Angiogenesis 20 3.3.2 Apoptosis 20 3.3.3 Mitosis 21 3.3.4 Topoisomerases and DNA polymerases 23 3.4 Current marine derived drugs in pre-clinical and clinical trials 23 3.5 Aim of the study 25 4. PUBLICATION 1: AAPTOS SUBERITOIDES 26 5. PUBLICATION 2: POLYCARPA AURATA 55 6. PUBLICATION 3: CALLYSPONGIA SP. 77 7. DISCUSSION 118 7.1. Isolated natural products from Aaptos suberitoides 118 7.1.1 Benzo[de][1,6]-naphthyridine alkaloids 118 7.2. Isolated natural products from Polycarpa aurata 121 7.2.1 Sulfur containing guanidine alkaloids 121 7.2.2 -carboline alkaloids 124 7.3.
    [Show full text]