Anti-Cct Theta Monoclonal Antibody, Clone PK/13/72/8K (CABT-54843RC) This Product Is for Research Use Only and Is Not Intended for Diagnostic Use

Total Page：16

File Type：pdf, Size：1020Kb

Anti-Cct Theta monoclonal antibody, clone PK/13/72/8k (CABT-54843RC) This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Product Overview This product recognises the theta polypeptide of the CCT chaperonin molecule complex. The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein. Western Blotting detects a band of approximately 60kD in lysates of heat shocked Hela cells. Specificity CCT THETA Isotype IgG2a Source/Host Rat Species Reactivity Bovine, Human, Mouse, Rabbit Clone PK/13/72/8k Conjugate Unconjugated Applications IP; WB Format Purified IgG - liquid Size 100 μg Preservative See individual product datasheet Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Warnings For research purposes only GENE INFORMATION Gene Name CCT8 chaperonin containing TCP1, subunit 8 (theta) [ Bos taurus (cattle) ] Official Symbol CCT8 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved Synonyms CCT8; T-complex protein 1 subunit theta; CCT-theta; TCP-1-theta; CCT THETA; Entrez Gene ID 281047 Protein Refseq NP_001028781.1 UniProt ID Q3ZCI9 Chromosome Location 1 Pathway Association of TriC/CCT with target proteins during biosynthesis; Chaperonin-mediated protein folding; Cooperation of Prefoldin andTriC/CCT in actin and tubulin folding; Folding of actin by CCT/TriC; Formation of tubulin folding intermediates by CCT/TriC; Metabolism of proteins; Prefoldin mediated transfer of substrate to CCT/TriC; Protein folding; Function ATP binding; unfolded protein binding; 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 2 © Creative Diagnostics All Rights Reserved.

Copy Link

Recommended publications

Identification of Differentially Expressed Genes in Human Bladder Cancer Through Genome-Wide Gene Expression Profiling
521-531 24/7/06 18:28 Page 521 ONCOLOGY REPORTS 16: 521-531, 2006 521 Identification of differentially expressed genes in human bladder cancer through genome-wide gene expression profiling KAZUMORI KAWAKAMI1,3, HIDEKI ENOKIDA1, TOKUSHI TACHIWADA1, TAKENARI GOTANDA1, KENGO TSUNEYOSHI1, HIROYUKI KUBO1, KENRYU NISHIYAMA1, MASAKI TAKIGUCHI2, MASAYUKI NAKAGAWA1 and NAOHIKO SEKI3 1Department of Urology, Graduate School of Medical and Dental Sciences, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-8520; Departments of 2Biochemistry and Genetics, and 3Functional Genomics, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan Received February 15, 2006; Accepted April 27, 2006 Abstract. Large-scale gene expression profiling is an effective CKS2 gene not only as a potential biomarker for diagnosing, strategy for understanding the progression of bladder cancer but also for staging human BC. This is the first report (BC). The aim of this study was to identify genes that are demonstrating that CKS2 expression is strongly correlated expressed differently in the course of BC progression and to with the progression of human BC. establish new biomarkers for BC. Specimens from 21 patients with pathologically confirmed superficial (n=10) or Introduction invasive (n=11) BC and 4 normal bladder samples were studied; samples from 14 of the 21 BC samples were subjected Bladder cancer (BC) is among the 5 most common to microarray analysis. The validity of the microarray results malignancies worldwide, and the 2nd most common tumor of was verified by real-time RT-PCR. Of the 136 up-regulated the genitourinary tract and the 2nd most common cause of genes we detected, 21 were present in all 14 BCs examined death in patients with cancer of the urinary tract (1-7).
[Show full text]
A Survey of the Ce-Tubulin Gene Family in Chicken: Unexpected Sequence Heterogeneity in the Polypeptides Encoded by Five Expressed Genes
The EMBO Journal vol.7 no.4 pp.931 -940, 1988 A survey of the ce-tubulin gene family in chicken: unexpected sequence heterogeneity in the polypeptides encoded by five expressed genes Lisa F.Pratt1 and Don W.Cleveland encoding both a- and ,B-tubulin lay beneath this heterogeneity of microtubule utilization (see Cleveland and Sullivan, 1985; Department of Biological Chemistry, The Johns Hopkins University Cleveland, 1987). For ,3-tubulin, extensive analyses in School of Medicine, 725 N. Wolfe St., Baltimore, MD 21205, USA chicken (Sullivan and Cleveland, 1984; Sullivan et al., 1985, 'Present address: Division of Clinical Immunology, Scripps Clinic and 1986a,b; Murphy et al., 1987; Montiero and Cleveland, Research Foundation, 10666 N. Torrey Pines Road, La Jolla, CA 92037, USA 1988), mouse (Lewis et al., 1985a; Sullivan and Cleveland, 1986; Wang et al., 1986) and human (Hall et al., 1983; Lee Communicated by K.Weber et al., 1983; Lewis et al., 1985b; Sullivan and Cleveland, 1986) have revealed six functional vertebrate f-tubulins. At To characterize the a-tubulin gene family in chicken, we least four [and probably five-see Lopata and Cleveland have isolated five chicken a-tubulin genes and determined (1987)] of these represent distinct ,B-tubulin isotypes whose the majority of the sequences of the encoded polypep- sequences (particularly within a carboxy-terminal variable tides. Three of these (cA3, ca5/6 and ca8) encode novel, domain) have been evolutionarily conserved (Sullivan and expressed a-tubulins that have not previously been Cleveland, 1986; Wang et al., 1986). analyzed, whereas one gene segment is a pseudogene and For a-tubulin, the analyses are less complete.
[Show full text]
Ultraconserved Elements Are Associated with Homeostatic Control of Splicing Regulators by Alternative Splicing and Nonsense-Mediated Decay
Downloaded from genesdev.cshlp.org on September 24, 2021 - Published by Cold Spring Harbor Laboratory Press Ultraconserved elements are associated with homeostatic control of splicing regulators by alternative splicing and nonsense-mediated decay Julie Z. Ni,1 Leslie Grate,1 John Paul Donohue,1 Christine Preston,2 Naomi Nobida,2 Georgeann O’Brien,2 Lily Shiue,1 Tyson A. Clark,3 John E. Blume,3 and Manuel Ares Jr.1,2,4 1Center for Molecular Biology of RNA and Department of Molecular, Cell, and Developmental Biology, University of California at Santa Cruz, Santa Cruz, California 95064, USA; 2Hughes Undergraduate Research Laboratory, University of California at Santa Cruz, Santa Cruz, California 95064, USA; 3Affymetrix, Inc., Santa Clara, California 95051, USA Many alternative splicing events create RNAs with premature stop codons, suggesting that alternative splicing coupled with nonsense-mediated decay (AS-NMD) may regulate gene expression post-transcriptionally. We tested this idea in mice by blocking NMD and measuring changes in isoform representation using splicing-sensitive microarrays. We found a striking class of highly conserved stop codon-containing exons whose inclusion renders the transcript sensitive to NMD. A genomic search for additional examples identified >50 such exons in genes with a variety of functions. These exons are unusually frequent in genes that encode splicing activators and are unexpectedly enriched in the so-called “ultraconserved” elements in the mammalian lineage. Further analysis show that NMD of mRNAs for splicing activators such as SR proteins is triggered by splicing activation events, whereas NMD of the mRNAs for negatively acting hnRNP proteins is triggered by splicing repression, a polarity consistent with widespread homeostatic control of splicing regulator gene expression.
[Show full text]
Novel Potential ALL Low-Risk Markers Revealed by Gene
Leukemia (2003) 17, 1891–1900 & 2003 Nature Publishing Group All rights reserved 0887-6924/03 $25.00 www.nature.com/leu BIO-TECHNICAL METHODS (BTS) Novel potential ALL low-risk markers revealed by gene expression profiling with new high-throughput SSH–CCS–PCR J Qiu1,5, P Gunaratne2,5, LE Peterson3, D Khurana2, N Walsham2, H Loulseged2, RJ Karni1, E Roussel4, RA Gibbs2, JF Margolin1,6 and MC Gingras1,6 1Texas Children’s Cancer Center and Department of Pediatrics; 2Human Genome Sequencing Center, Department of Molecular and Human Genetics; 3Department of Internal Medicine; 1,2,3 are all departments of Baylor College of Medicine, Baylor College of Medicine, Houston, TX, USA; and 4BioTher Corporation, Houston, TX, USA The current systems of risk grouping in pediatric acute t(1;19), BCR-ABL t(9;22), and MLL-AF4 t(4;11).1 These chromo- lymphoblastic leukemia (ALL) fail to predict therapeutic suc- somal modifications and other clinical findings such as age and cess in 10–35% of patients. To identify better predictive markers of clinical behavior in ALL, we have developed an integrated initial white blood cell count (WBC) define pediatric ALL approach for gene expression profiling that couples suppres- subgroups and are used as diagnostic and prognostic markers to sion subtractive hybridization, concatenated cDNA sequencing, assign specific risk-adjusted therapies. For instance, 1.0 to 9.9- and reverse transcriptase real-time quantitative PCR. Using this year-old patients with none of the determinant chromosomal approach, a total of 600 differentially expressed genes were translocation (NDCT) mentioned above but with a WBC higher identified between t(4;11) ALL and pre-B ALL with no determi- than 50 000 cells/ml are associated with higher risk group.2 nant chromosomal translocation.
[Show full text]
Novel Targets of Apparently Idiopathic Male Infertility
International Journal of Molecular Sciences Review Molecular Biology of Spermatogenesis: Novel Targets of Apparently Idiopathic Male Infertility Rossella Cannarella * , Rosita A. Condorelli , Laura M. Mongioì, Sandro La Vignera * and Aldo E. Calogero Department of Clinical and Experimental Medicine, University of Catania, 95123 Catania, Italy; [email protected] (R.A.C.); [email protected] (L.M.M.); [email protected] (A.E.C.) * Correspondence: [email protected] (R.C.); [email protected] (S.L.V.) Received: 8 February 2020; Accepted: 2 March 2020; Published: 3 March 2020 Abstract: Male infertility affects half of infertile couples and, currently, a relevant percentage of cases of male infertility is considered as idiopathic. Although the male contribution to human fertilization has traditionally been restricted to sperm DNA, current evidence suggest that a relevant number of sperm transcripts and proteins are involved in acrosome reactions, sperm-oocyte fusion and, once released into the oocyte, embryo growth and development. The aim of this review is to provide updated and comprehensive insight into the molecular biology of spermatogenesis, including evidence on spermatogenetic failure and underlining the role of the sperm-carried molecular factors involved in oocyte fertilization and embryo growth. This represents the first step in the identification of new possible diagnostic and, possibly, therapeutic markers in the field of apparently idiopathic male infertility. Keywords: spermatogenetic failure; embryo growth; male infertility; spermatogenesis; recurrent pregnancy loss; sperm proteome; DNA fragmentation; sperm transcriptome 1. Introduction Infertility is a widespread condition in industrialized countries, affecting up to 15% of couples of childbearing age [1]. It is defined as the inability to achieve conception after 1–2 years of unprotected sexual intercourse [2].
[Show full text]
TCP1 Theta (CCT8) (NM 006585) Human Tagged ORF Clone Product Data
OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for RC210044 TCP1 theta (CCT8) (NM_006585) Human Tagged ORF Clone Product data: Product Type: Expression Plasmids Product Name: TCP1 theta (CCT8) (NM_006585) Human Tagged ORF Clone Tag: Myc-DDK Symbol: CCT8 Synonyms: C21orf112; Cctq; D21S246; PRED71 Vector: pCMV6-Entry (PS100001) E. coli Selection: Kanamycin (25 ug/mL) Cell Selection: Neomycin This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 5 TCP1 theta (CCT8) (NM_006585) Human Tagged ORF Clone – RC210044 ORF Nucleotide >RC210044 ORF sequence Sequence: Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTTCACGTTCCCAAGGCTCCGGGCTTTGCCCAGATGCTCAAGGAGGGAGCGAAACACTTTTCAG GATTAGAAGAGGCTGTGTATAGAAACATACAAGCTTGCAAGGAGCTTGCCCAAACCACTCGTACAGCATA TGGACCAAATGGAATGAACAAAATGGTTATCAACCACTTGGAGAAGTTGTTTGTGACAAACGATGCAGCA ACTATTTTAAGAGAACTAGAAGTACAGCATCCTGCTGCAAAAATGATTGTAATGGCTTCTCATATGCAAG AGCAAGAAGTTGGAGATGGCACAAACTTTGTTCTGGTATTTGCTGGAGCTCTCCTGGAATTAGCTGAAGA ACTTCTGAGGATTGGCCTGTCAGTTTCAGAGGTCATAGAAGGTTATGAAATAGCCTGCAGAAAAGCTCAT GAGATTCTTCCTAATTTGGTATGTTGTTCTGCAAAAAACCTTCGAGATATTGATGAAGTCTCATCTCTAC TTCGTACCTCCATAATGAGTAAACAATATGGTAATGAAGTATTTCTGGCCAAGCTTATTGCTCAGGCATG CGTATCTATTTTTCCTGATTCCGGCCATTTCAATGTTGATAACATCAGAGTTTGTAAAATTCTGGGCTCT
[Show full text]
Title: a Yeast Phenomic Model for the Influence of Warburg Metabolism on Genetic
bioRxiv preprint doi: https://doi.org/10.1101/517490; this version posted January 15, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 1 Title Page: 2 3 Title: A yeast phenomic model for the influence of Warburg metabolism on genetic 4 buffering of doxorubicin 5 6 Authors: Sean M. Santos1 and John L. Hartman IV1 7 1. University of Alabama at Birmingham, Department of Genetics, Birmingham, AL 8 Email: [email protected], [email protected] 9 Corresponding author: [email protected] 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 1 bioRxiv preprint doi: https://doi.org/10.1101/517490; this version posted January 15, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 26 Abstract: 27 Background: 28 Saccharomyces cerevisiae represses respiration in the presence of adequate glucose, 29 mimicking the Warburg effect, termed aerobic glycolysis. We conducted yeast phenomic 30 experiments to characterize differential doxorubicin-gene interaction, in the context of 31 respiration vs. glycolysis. The resulting systems level biology about doxorubicin 32 cytotoxicity, including the influence of the Warburg effect, was integrated with cancer 33 pharmacogenomics data to identify potentially causal correlations between differential 34 gene expression and anti-cancer efficacy.
[Show full text]
Datasheet: MCA2180 Product Details
Datasheet: MCA2180 Description: RAT ANTI CCT THETA Specificity: CCT THETA Format: Purified Product Type: Monoclonal Antibody Clone: PK/13/72/8k Isotype: IgG2a Quantity: 0.1 mg Product Details Applications This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.bio-rad-antibodies.com/protocols. Yes No Not Determined Suggested Dilution Flow Cytometry Immunohistology - Frozen Immunohistology - Paraffin ELISA Immunoprecipitation Western Blotting 1/250 - 1/500 Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Species Cross Reacts with: Mouse, Rabbit, Human, Bovine Reactivity N.B. Antibody reactivity and working conditions may vary between species. Product Form Purified IgG - liquid Preparation Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant Buffer Solution Phosphate buffered saline Preservative 0.09% Sodium Azide Stabilisers Carrier Free Yes Approx. Protein IgG concentration 1.0 mg/ml Concentrations External Database UniProt: Links Q3ZCI9 Related reagents Page 1 of 3 Entrez Gene: 281047 CCT8 Related reagents Specificity Rat anti CCT theta antibody, clone PK/13/72/8k recognises the theta polypeptide of the CCT chaperonin molecule complex, also known as T-complex protein 1 subunit theta, CCT8 or CCTθ, a 547 amino acid ~:60kDa molecular chaperone.
[Show full text]
The Chaperonin CCT8 Controls Proteostasis Essential for T Cell Maturation, Selection, and Function
ARTICLE https://doi.org/10.1038/s42003-021-02203-0 OPEN The chaperonin CCT8 controls proteostasis essential for T cell maturation, selection, and function Bergithe E. Oftedal 1,2,11, Stefano Maio 1,11, Adam E. Handel 1, Madeleine P. J. White3, Duncan Howie4, Simon Davis 5, Nicolas Prevot1, Ioanna A. Rota1, Mary E. Deadman1, Benedikt M. Kessler 5, ✉ Roman Fischer 5, Nikolaus S. Trede6, Erdinc Sezgin 7,10, Rick M. Maizels3 & Georg A. Holländer 1,8,9 T cells rely for their development and function on the correct folding and turnover of proteins generated in response to a broad range of molecular cues. In the absence of the eukaryotic 1234567890():,; type II chaperonin complex, CCT, T cell activation induced changes in the proteome are compromised including the formation of nuclear actin filaments and the formation of a normal cell stress response. Consequently, thymocyte maturation and selection, and T cell homeostatic maintenance and receptor-mediated activation are severely impaired. In the absence of CCT-controlled protein folding, Th2 polarization diverges from normal differ- entiation with paradoxical continued IFN-γ expression. As a result, CCT-deficient T cells fail to generate an efficient immune protection against helminths as they are unable to sustain a coordinated recruitment of the innate and adaptive immune systems. These findings thus demonstrate that normal T cell biology is critically dependent on CCT-controlled proteostasis and that its absence is incompatible with protective immunity. 1 Developmental Immunology, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, UK. 2 Department of Clinical Science, University of Bergen, Bergen, Norway, K.G.
[Show full text]
Supplementary Appendix for Expression Quantitative Trait Locus Mapping in Pulmonary Arterial Hypertension Anna Ulrich Et Al
Supplementary Appendix for Expression quantitative trait locus mapping in pulmonary arterial hypertension Anna Ulrich et al. RNA sequencing and transcript abundance estimation ..................................................................... 2 eQTL validation procedure .................................................................................................................. 2 eQTL studies used for validation ......................................................................................................... 3 Supplementary Tables ........................................................................................................................ 4 RNA sequencing and transcript abundance estimation Whole blood (3ml) was collected in Tempus™ Blood RNA Tubes, which were stored at -80 oC until required. RNA was extracted using a Maxwell robotic system (Promega). Samples with a 260/230 ratio >1.5 and a 260/280 ratio in the range 1.9-2.1 were further quality checked by Bioanalyser and those achieving a minimum RNA Integrity Number (RIN) of 7 were submitted for sequencing. Globin- Zero Gold rRNA Removal Kits (Illumina Inc, San Diego, CA) were used to remove ribosomal RNA contamination from whole blood RNA samples. 75bp paired-end sequencing on a Hiseq4000 was performed on pooled libraries of ~80 samples. Fastq files (raw reads from RNAseq) were analysed using Salmon v0.9.1 (Patro et al., 2017) and GENCODE release 28 to produce transcript abundance estimates which were converted to gene expression data using tximport in R
[Show full text]
A Master Autoantigen-Ome Links Alternative Splicing, Female Predilection, and COVID-19 to Autoimmune Diseases
bioRxiv preprint doi: https://doi.org/10.1101/2021.07.30.454526; this version posted August 4, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. A Master Autoantigen-ome Links Alternative Splicing, Female Predilection, and COVID-19 to Autoimmune Diseases Julia Y. Wang1*, Michael W. Roehrl1, Victor B. Roehrl1, and Michael H. Roehrl2* 1 Curandis, New York, USA 2 Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, USA * Correspondence: [email protected] or [email protected] 1 bioRxiv preprint doi: https://doi.org/10.1101/2021.07.30.454526; this version posted August 4, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. Abstract Chronic and debilitating autoimmune sequelae pose a grave concern for the post-COVID-19 pandemic era. Based on our discovery that the glycosaminoglycan dermatan sulfate (DS) displays peculiar affinity to apoptotic cells and autoantigens (autoAgs) and that DS-autoAg complexes cooperatively stimulate autoreactive B1 cell responses, we compiled a database of 751 candidate autoAgs from six human cell types. At least 657 of these have been found to be affected by SARS-CoV-2 infection based on currently available multi-omic COVID data, and at least 400 are confirmed targets of autoantibodies in a wide array of autoimmune diseases and cancer.
[Show full text]
Survey of Human Chromosome 21 Gene Expression Effects on Early Development in Danio Rerio
G3: Genes|Genomes|Genetics Early Online, published on May 14, 2018 as doi:10.1534/g3.118.200144 INVESTIGATIONS Survey of human chromosome 21 gene expression effects on early development in Danio rerio Sarah Edie∗,, Norann A. Zaghloul†, Carmen C. Leitch†, Donna K. Klinedinst‡, Janette Lebron‡, Joey F. Thole∗, Andrew S McCallion∗, Nicholas Katsanis§ and Roger H. Reeves∗,‡,1 ∗Johns Hopkins University School of Medicine, Institute of Genetic Medicine, Baltimore, MD, †University of Maryland School of Medicine, Department of Medicine, Division of Endocrinology, Diabetes and Nutrition, ‡Johns Hopkins University School of Medicine, Department of Physiology, Baltimore,MD, §Center for Human Disease Modeling, Duke University ABSTRACT Trisomy for human chromosome 21 (Hsa21) results in Down syndrome (DS), one of the most KEYWORDS genetically complex conditions compatible with human survival. Assessment of the physiological consequences Hsa21 of dosage-driven overexpression of individual Hsa21 genes during early embryogenesis and the resulting Zebrafish contributions to DS pathology in mammals are not tractable in a systematic way. A recent study looked Over expression loss-of-function of C. elegans orthologues of Hsa21 genes and identified ten candidates with behavioral phenotypes, but the equivalent over-expression experiment has not been done. We turned to zebrafish as a developmental model and, using a number of surrogate phenotypes, we screened Hsa21 genes for dosage sensitive effects on early embyrogenesis. We prepared a library of 164 cDNAs of conserved protein coding genes, injected mRNA into early embryos and evaluated up to 5 days post-fertilization (dpf). Twenty-four genes produced a gross morphological phenotype, 11 of which could be reproduced reliably.
[Show full text]