DOCSLIB.ORG

Tracing Transgenic Maize As Affected by Breadmaking Process and Raw Material for the Production of a Traditional Maize Bread, Broa ⇑ Telmo J.R

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Tracing Transgenic Maize As Affected by Breadmaking Process and Raw Material for the Production of a Traditional Maize Bread, Broa ⇑ Telmo J.R Food Chemistry 138 (2013) 687–692 Contents lists available at SciVerse ScienceDirect Food Chemistry journal homepage: www.elsevier.com/locate/foodchem Tracing transgenic maize as affected by breadmaking process and raw material for the production of a traditional maize bread, broa ⇑ Telmo J.R. Fernandes, M. Beatriz P.P. Oliveira, Isabel Mafra REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, Porto 4050-313, Portugal article info abstract Article history: Broa is a maize bread highly consumed and appreciated, especially in the north and central zones of Por- Received 15 June 2012 tugal. In the manufacturing of broa, maize flour and maize semolina might be used, besides other cereals Received in revised form 28 September 2012 such as wheat and rye. Considering the needs for genetically modified organism (GMO) traceability in Accepted 24 October 2012 highly processed foods, the aim of this work was to assess DNA degradation, DNA amplification and Available online 12 November 2012 GMO quantification along breadmaking process of broa. DNA degradation was noticed by its decrease of integrity after dough baking and in all parts of bread sampling. The PCR amplification results of Keywords: extracted DNA from the three distinct maize breads (broa 1, 2 and 3) showed that sequences for maize Maize bread invertase gene and for events MON810 and TC1507 were easily detected with strong products. Real-time GMO detection DNA degradation PCR revealed that quantification of GMO was feasible in the three different breads and that sampling Processing location of baked bread might have a limited influence since the average quantitative results of both Traceability events after baking were very close to the actual values in the case of broa 1 (prepared with maize sem- Quantitative real-time PCR olina). In the other two maize breads subjected to the same baking treatment, the contents of MON810 maize were considerably underestimated, leading to the conclusion that heat-processing was not the responsible parameter for that distortion, but the size of particle and mechanical processing of raw maize play also a major role in GMO quantification. Ó 2012 Elsevier Ltd. All rights reserved. 1. Introduction (EU), maize has the highest number of authorised GM events (21) for food and feed (GMO Compass, GMO Database, Genetically Mod- Maize (Zea mays L.) plays a major role in nutrition in many ified Food, 2012). Examples of authorised transgenic maize events countries. It is a cereal widely used for human nutrition as a source are the MON810 maize, which contains the cry1Ab gene inserted to of oil, flour and starch. Maize is the basis for the production of sev- confer insect resistance, and the TC1507 maize, which contains the eral foods, such as polenta, bread, tortillas, snacks, cornflakes, etc. cry1Fa2 and pat genes inserted to confer insect resistance and tol- In the production of bread, it is also used as wheat flour erance to the herbicide glufosinate ammonium, respectively. replacement. MON810 was introduced as an authorised maize event in the EU Broa is a Portuguese traditional maize bread highly consumed, in 1998, being actually under a renewal process for authorisation especially in the north and central zones of Portugal and still plays as food and feed and cultivation. The maize event TC1507 is actu- an important economic and social role in rural communities of the ally authorised under the scope of application for food and feed country (Brites, Trigo, Santos, Collar, & Rosell, 2010; Lino, Silva, since 2005, but not yet for cultivation (GMO Compass, 2012). Both Pena, Férnandez, & Mañes 2007; Vaz Patto, Moreira, Carvalho, & events have been reported as the most frequently found in foods Pego, 2007). The traditional manufacturing of broa consists of add- available in the Portuguese retail market (Fernandes, 2011; Fer- ing sieved maize flour (mixed with wheat or rye flours), hot water, nandes, Costa, Oliveira, & Mafra, 2012). yeast and leavened dough. After mixing, resting and proofing, the In EU, under Regulations (EC) No. 1829/2003 and 1830/2003 dough is baked in an oven (temperature over 200 °C) resulting in regarding the authorisation and traceability of food and feed de- a highly accepted foodstuff, mostly due to its distinctive sensorial rived from genetically modified organisms (GMO), labelling for characteristics (Brites et al., 2010). food products containing more than 0.9% authorised GM material Maize is the second most cultivated genetically modified (GM) is mandatory. To monitor and verify the compliance of labelling, crop, corresponding to 32% of the global biotechnological area of appropriate testing methods to detect and identify GM events in 160 million hectares in 2011 (James, 2011). In the European Union processed foodstuffs are needed. Genomic DNA extraction fol- lowed by amplification of target sequences (endogenous and ⇑ Corresponding author. Tel.: +351 220428640; fax: +351 226093390. event/construct specific) by qualitative and quantitative polymer- E-mail address: [email protected] (I. Mafra). ase chain reaction (PCR) are the most widely accepted methods 0308-8146/$ - see front matter Ó 2012 Elsevier Ltd. All rights reserved. http://dx.doi.org/10.1016/j.foodchem.2012.10.068 688 T.J.R. Fernandes et al. / Food Chemistry 138 (2013) 687–692 for detecting and quantifying levels of GM traits in foodstuffs (Gry- Table 1 son, Dewettinck, & Messens, 2007; Shokere, Holden, & Jenkins, Composition of the three different maize breads prepared. 2009; van der Colff & Podivinsky, 2008). Real-time quantitative Ingredients (g) Broa 1 Broa 2 Broa 3 PCR represents the most powerful means of quantifying GM mate- GM maize semolina 400 – – rial in agricultural and food products, due to its high sensitivity, GM maize flour – 100 150 specificity and reproducibility (Gryson, 2010; Mafra, Ferreira, & Wheat flour (type 65) 300 150 150 Oliveira, 2008). However, applicability of DNA-based methods for Maize flour (type 70) – 100 50 Rye flour (type 130) 100 50 50 GMO detection and quantification depends on the quality and Bread improver 8 4 4 quantity of DNA. Salt 12 6 6 Food processing and consequent DNA degradation, can affect Sugar 8 4 4 DNA recovery and the efficiency of PCR and, therefore, the quality Dry yeast 24 12 12 of analytical results, leading to under- or overestimation of GMO Total + Water 1000 500 500 content (Bergerová, Hrncˇírová, Stankovská, Lopašovská, & Siekel, 2010; Gryson, 2010). The degree of sample processing and the DNA extraction method used may lead to DNA fragment damage, was manually moulded in balls and baked in the oven at 240 °C, which can be caused by exposure to heat or pressure, enzymatic during 30 min. degradation by nucleases, chemical agents, water activity, shear During the preparation of maize bread two samples of dough forces and pH values (Gryson, 2010; Gryson, Messens, & Dewet- were taken: before and after leavening. In the final baked breads, tinck, 2008). Food manufacturing comprises a large number of three samples were taken from different bread location: crust, un- complex processing steps, which contribute to a particular envi- der crust and middle soft part of the bread. Each sample was sep- ronment where DNA undergoes deterioration and proteins are arately ground and homogenised in a Grindomix GM200 (Retsch, denatured (Vijayakumar, Martin, Gowda, & Prakash, 2009). Cook- Haan, Germany). ing and baking are essentially heat processes that may break down DNA, whereas the thermal treatment with temperatures over 2.3. DNA extraction 200 °C considerably reduces the size of the extracted DNA frag- ments (Bergerová, Godálová, & Siekel, 2011; Gryson, 2010). DNA was extracted using the Wizard method as described by Considering the few number of reports assessing the influence Mafra, Silva, Moreira, Silva, and Oliveira (2008), with minor modi- of the thermal treatment on DNA degradation and PCR amplifica- fications. To each sample (100–150 mg) transferred to a 2 mL ster- tion for GMO detection and quantification in maize-derived food- ile reaction tube, 860 lL of extraction buffer (10 mM Tris, 150 mM stuffs, this study aimed at evaluating the effect of breadmaking NaCl, 2 mM EDTA, 1% SDS), 100 lL of 5 M guanidine hydrochloride of maize bread manufactured with known amounts of transgenic solution and 40 lL of proteinase K solution (20 mg/mL) were maize. Therefore, different traditional maize breads (broa) were added. The mixtures were vigorously vortexed and incubated in a prepared and samples were taken before and after the steps of thermal block (Thermomixer Compact, Eppendorf AG, Hamburg, sourdough leavening and baking to assess DNA degradation and Germany) at 60 °C for 3 h with continuous stirring. After incuba- amplification, with special focus on real-time quantitative PCR tion, the mixtures were centrifuged (15 min, 18,514g at 4 °C), for GMO quantification. and 500 lL of supernatant were collected and mixed with 1 mL of WizardÒ DNA purification resin (Promega, Madison, WI, USA). Ò 2. Material and methods Each mixture was then pushed through a Wizard column (Prome- ga, Madison, WI, USA) mounted with a 2 mL syringe. The DNA-re- 2.1. GM maize material sin mix was washed twice with 2 mL isopropanol solution (80% v/ v). The column was dried for 5 min at room temperature and Certified reference materials from the Institute for Reference placed in a new sterile reaction tube. After incubation for 1 min Materials and Measurements (IRMM, Geel, Belgium) were used with 100 lL of Tris–EDTA buffer (10 mM Tris, 1 mM EDTA) at as standards (Fluka, Buchs, Switzerland) containing 1% and 5% for 70 °C, the column was eluted by centrifugation (1 min, 10,000g). the evaluation of MON810 maize event and 1% and 10% for All the extracts were kept at À20 °C until further analysis. TC1507 event. To prepare incurred maize breads with GM maize, two different 2.4.
Load more

Recommended publications
  • Maize Flour Parameters That Are Related to the Consumer Perceived
    a Food Science and Technology ISSN 0101-2061 DDOI http://dx.doi.org/10.1590/1678-457X.6674 Maize flour parameters that are related to the consumer perceived quality of ‘broa’ specialty bread Bruna CARBAS1, Maria Carlota VAZ-PATTD2, Maria Rosário BRDNZE2,3,4, Andreia BENTD-DA-SILVA2, Maria João TRIGD1, Carla BRITES1* Abstract A wide range of quality parameters have been used to describe maize flours for food use, but there is no general agreement about the most suitable parameters for breadmaking. The objective of this study was to identify the maize flour parameters related to the consumer perceived quality of Portuguese broa bread (more than 50% maize flour). The influence of eleven maize landraces was assessed and compared with commercial flour using baking tests. The broa were evaluated by instrumental (colour, firmness) and sensory hedonic analysis with a consumer panel of 52 assessors. The broa sensory analysis revealed similar assessments among landraces and the lowest scores for commercial flour. The flour particle size distribution is the major influence, with commercial flour showing the highest mean diameter and a large particle distribution range. Broa consumer panel linkage associations and specific sensory descriptors have been identified; age as an influence on colour, cohesiveness, and source region as an influence on appearance and texture. Keywords: broa; maize; bread; consumer parameters; sensory analysis. Practical Application: Maize landrace flours have a narrow particle size distribution and a positive impact on broa bread quality. 1 Introduction Portugal was one the first European nations to adopt maize A general consensus has been reached regarding the (Zea mays L.) in its agricultural system more than five centuries classification of breads according to the degree of hydration of ago (Vaz Patto et al., 2009).
    [Show full text]
  • Maize Based Gluten Free Bread: Influence of Processing
    1 MAIZE BASED GLUTEN FREE BREAD: INFLUENCE OF PROCESSING 2 PARAMETERS ON SENSORY AND INSTRUMENTAL QUALITY 3 4 5 Carla Brites1*, Maria João Trigo1, Carla Santos1, Concha Collar2, Cristina M. 6 Rosell2 7 8 1Instituto Nacional dos Recursos Biológicos, I.P., L-INIA, Unidade Tecnologia 9 Alimentar, Quinta do Marquês, 2784-505 Oeiras, Portugal. 10 2Institute of Agrochemistry and Food Technology (IATA-CSIC). PO Box 73, 46100- 11 Burjasot (Valencia), Spain. 12 13 Running title: Maize based gluten free breads 14 15 * Corresponding author. Tel: +351214403684; fax:+351214413208 16 E-mail address: [email protected] 1 17 Abstract 18 The performance of maize bread with spongy texture is still a technological challenge due 19 to the absence of a natural network required for holding the carbon dioxide released 20 during the fermentation process. The objective of this research was to investigate the 21 influence of different maize varieties (regional and hybrid), milling process (electric and 22 water mill), formulation and processing variables on the sensory and instrumental 23 (specific volume, texture and colour) quality attributes of corn bread. For that purpose, the 24 traditional breadmaking process applied to the development of the ethnic Portuguese bread 25 (broa) obtained from composite maize-rye-wheat flour was modified to produce gluten- 26 free broa. Significant differences (p<0.05) between regional and hybrid maize were 27 detected in terms of protein, amylose, and maximum, minimum and final viscosities as 28 evaluated by Rapid Visco Analyser. Concerning the effect of milling process, the grinding 29 in a water mill occurs at slower rate than it does in the electrical mill, in consequence the 30 flour from water milling had lower ash content and higher maximum, minimum and final 31 viscosities than the one obtained from electrical milling.
    [Show full text]
  • The Food Culture of Portugal Part 1— a Unique Corner of Europe
    VOLUMEVOLUME XVI, XXXII, NUMBER NUMBER 4 4 FALL FALL 2000 2016 Quarterly Publication of the Culinary Historians of Ann Arbor The Food Culture of Portugal Part 1— A Unique Corner of Europe Photo: http://pasteisdebelem.pt/ Custard tarts called pastéis de Belém, available commercially since 1837, are one of the famous “convent sweets” of Portugal. These here are about to be devoured at the historic Casa Pastéis de Belém, a café in Lisbon that guards its secret recipe. REPAST VOLUME XXXII, NUMBER 4 FALL 2016 WHY PORTUGAL CAPTIVATED GEORGE ESTABROOK November 24 marks five years since the passing of George would observe and F. Estabrook (1942-2011), a CHAA member and Repast writer analyze centuries-old whose years of work in Portugal were the inspiration for this farming techniques to theme. My full obituary article about him was included in our identify practices, some of Winter 2012 issue. I really miss George’s energy and erudition, them ingenious, that and I’m dedicating these two issues in his memory. foster sustainability and that might be adaptable to George was a longtime professor of botany at the Univ. of more industrialized Michigan, where his two basic courses were “How People Use countries. For example, he Plants” and “Biology of Human Nutrition”. He also gave public studied how the fertility talks about “The Roots of Soul Food” and other topics, and for of shale soil is maintained the CHAA he spoke about “The History of Citrus Fruit” (Jan. by such traditional 2006) and “The Domestication and Spread of Bananas” (Jan. techniques as rotating 2009).
    [Show full text]
  • Occurrence of Fumonisins B1 and B2 in Broa, Typical Portuguese Maize Bread ⁎ C.M
    International Journal of Food Microbiology 118 (2007) 79–82 www.elsevier.com/locate/ijfoodmicro Short communication Occurrence of fumonisins B1 and B2 in broa, typical Portuguese maize bread ⁎ C.M. Lino a, , L.J.G. Silva a, A. Pena a, M. Fernández b, J. Mañes b a Group of Bromatology - CEF, Faculty of Pharmacy, University of Coimbra, 3000-295 Coimbra, Portugal b Laboratory of Bromatology and Toxicology, Faculty of Pharmacy, University of València, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, València, Spain Received 23 June 2006; received in revised form 26 February 2007; accepted 11 April 2007 Abstract Fumonisin B1 (FB1) and fumonisin B2 (FB2) are mycotoxins mainly produced by Fusarium verticillioides, and Fusarium proliferatum, fungi species most commonly isolated from maize. The natural occurrence of FB1 and FB2 in broa, typical Portuguese maize bread, was evaluated in 30 samples. Twenty five were found positive with levels ranging from 142 to 550 μgkg−1. The limit established by the European regulations was exceeded by 27% of the samples. The tolerable daily intake for fumonisin B1, and B2, alone or in combination, for all of the analysed samples, was lower than 2 μgkg−1 body weight per day established by the European Commission. © 2007 Elsevier B.V. All rights reserved. Keywords: Fumonisins B1 and B2; Maize bread; HPLC 1. Introduction et al., 1996). High FBs levels are frequently reported in maize or maize-based foods from many countries, such as Benin Fumonisin mycotoxins are produced by a limited number of (Fandohan et al., 2005), Korea (Park et al., 2005), China (Liu morphologically related Fusarium species, of which Fusarium et al., 2005), Brazil (Bittencourt et al., 2005), Nigeria (Bankole verticillioides (F.
    [Show full text]
  • Broa, an Ethnic Maize Bread, As a Source of Phenolic Compounds
    antioxidants Article Broa, an Ethnic Maize Bread, as a Source of Phenolic Compounds Andreia Bento-Silva 1,2,3 , Noélia Duarte 4 , Elsa Mecha 2 , Maria Belo 2, Ana Teresa Serra 5 , Maria Carlota Vaz Patto 2 and Maria Rosário Bronze 2,4,5,* 1 FCT NOVA, Faculdade de Ciências e Tecnologia, Campus da Caparica, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal; [email protected] 2 ITQB NOVA, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República, 2780-157 Oeiras, Portugal; [email protected] (E.M.); [email protected] (M.B.); [email protected] (M.C.V.P.) 3 FFULisboa, Faculdade de Farmácia da Universidade de Lisboa, Av. das Forças Armadas, 1649-019 Lisboa, Portugal 4 iMed.ULisboa, Instituto de Investigação do Medicamento, Faculdade de Farmácia, Universidade de Lisboa, Avenida Prof. Gama Pinto, 1649-003 Lisboa, Portugal; [email protected] 5 iBET, Instituto de Biologia Experimental e Tecnológica, Avenida da República, Quinta-do-Marquês, Estação Agronómica Nacional, Apartado 12, 2780-157 Oeiras, Portugal; [email protected] * Correspondence: [email protected] Abstract: Maize is an important source of phenolic compounds, specially hydroxycinnamic acids, which are widely known for their antioxidant activity and associated health benefits. However, these effects depend on their bioaccessibility, which is influenced by the different techniques used for food processing. Several traditional products can be obtained from maize and, in Portugal, it is used for the production of an ethnic bread called broa. In order to evaluate the effect of processing Citation: Bento-Silva, A.; Duarte, N.; Mecha, E.; Belo, M.; Serra, A.T.; Vaz on maize phenolic composition, one commercial hybrid and five open-pollinated maize flours and Patto, M.C.; Bronze, M.R.
    [Show full text]
  • Bread Bread Is a Staple Food Prepared by Baking a Dough of Flour and Water
    Bread Bread is a staple food prepared by baking a dough of flour and water.[1] It may be leavened or unleavened. Salt, fat and a leavening agent such as yeast are common ingredients, though breads may contain a range of other ingredients: milk, egg, sugar, spice, fruit(such as raisins), vegetables (such as onion), nuts (such as walnuts) or seeds (such as poppy seeds). Bread is one of the oldest prepared foods, dating back to the Neolithic era. The development of leavened bread can probably also be traced to prehistoric times. Fresh bread is prized for its taste, aroma and texture. Retaining its freshness is important to keep it appetizing. Bread that has stiffened or dried past its prime is said to be stale. Modern bread is sometimes wrapped in paper or plastic film, or stored in an airtight container such as a breadbox to keep it fresh longer. Bread that is kept in warm, moist environments is prone to the growth of mold. Bread kept at low temperatures, for example, in a refrigerator, will develop mold growth more slowly than bread kept at room temperature. However, unwrapped bread kept in a typical household refrigerator will turn stale quickly due to the low humidityof the air. The inner, soft part of bread is known to bakers and other culinary professionals as the crumb, which is not to be confused with small bits of bread that often fall off, called crumbs. The outer hard portion of bread is called the crust. Usage Bread can be served at any temperature. Once baked, it can subsequently be toasted.
    [Show full text]
  • Consumer-Driven Improvement of Maize Bread Formulations with Legume Fortification
    foods Article Consumer-Driven Improvement of Maize Bread Formulations with Legume Fortification Luís M. Cunha 1, Susana C. Fonseca 1, Rui C. Lima 2, José Loureiro 3, Alexandra S. Pinto 4, M. Carlota Vaz Patto 5 and Carla Brites 4,* 1 GreenUPorto & LAQV/REQUIMTE, DGAOT, Faculdade de Ciências da Universidade do Porto, Campus de Vairão, Rua da Agrária 747, 4485-646 Vila do Conde, Portugal 2 Sense Test, Lda., R. Zeferino Costa 341, 4400-345 Vila Nova de Gaia, Portugal 3 Patrimvs Indústria, SA, Zona Industrial da Abrunheira, 33/34 Apt 184, Vila Chã, 6270-187 Guarda, Portugal 4 INIAV-Instituto Nacional de Investigação Agrária e Veterinária, 2780-157 Oeiras, Portugal 5 ITQB NOVA-Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av República, Apartado 127, 2781-901 Oeiras, Portugal * Correspondence: [email protected]; Tel.: +351-214-403-500 Received: 24 May 2019; Accepted: 25 June 2019; Published: 29 June 2019 Abstract: The fortification of maize bread with legume flour was explored in order to increase the protein content of the traditional Portuguese bread ‘broa’, comprised of more than 50% maize flour. The optimization of legume incorporation (pea, chickpea, faba bean, lentil), considering the influence of different maize flours (traditional-white, traditional-yellow, hybrid-white, hybrid-yellow), on consumer liking and sensory profiling of ‘broa’ was studied. A panel of 60 naïve tasters evaluated twenty different breads, divided in four sets for each legume flour fortification, each set including four breads with varying maize flour and a control (no legume). Tasters evaluated overall liking and the sensory profile through a check-all-that-apply ballot.
    [Show full text]
  • Broa’ Specialty Bread Ciência E Tecnologia De Alimentos, Vol
    Ciência e Tecnologia de Alimentos ISSN: 0101-2061 [email protected] Sociedade Brasileira de Ciência e Tecnologia de Alimentos Brasil CARBAS, Bruna; VAZ-PATTO, Maria Carlota; BRONZE, Maria Rosário; BENTO-DA- SILVA, Andreia; João TRIGO, Maria; BRITES, Carla Maize flour parameters that are related to the consumer perceived quality of ‘broa’ specialty bread Ciência e Tecnologia de Alimentos, vol. 36, núm. 2, abril-junio, 2016, pp. 259-267 Sociedade Brasileira de Ciência e Tecnologia de Alimentos Campinas, Brasil Available in: http://www.redalyc.org/articulo.oa?id=395946786007 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative a Food Science and Technology ISSN 0101-2061 DDOI http://dx.doi.org/10.1590/1678-457X.6674 Maize flour parameters that are related to the consumer perceived quality of ‘broa’ specialty bread Bruna CARBAS1, Maria Carlota VAZ-PATTD2, Maria Rosário BRDNZE2,3,4, Andreia BENTD-DA-SILVA2, Maria João TRIGD1, Carla BRITES1* Abstract A wide range of quality parameters have been used to describe maize flours for food use, but there is no general agreement about the most suitable parameters for breadmaking. The objective of this study was to identify the maize flour parameters related to the consumer perceived quality of Portuguese broa bread (more than 50% maize flour). The influence of eleven maize landraces was assessed and compared with commercial flour using baking tests. The broa were evaluated by instrumental (colour, firmness) and sensory hedonic analysis with a consumer panel of 52 assessors.
    [Show full text]
  • Quality Characteristics of Maize Flours and Breads
    International Journal of Food Engineering Vol. 2, No. 2, December 2016 Quality Characteristics of Maize Flours and Breads Paula M. R. Correia Department of Food Industry, CI&DETS/ESAV, Polytechnic Institute of Viseu, Viseu, Portugal Email: [email protected] Andreia M. Soares Department of Food Industry, Agrarian School of Viseu, Viseu, Portugal Email: [email protected] Carla Brites Instituto Nacional de Investigação Agrária e Veterinária, Oeiras, Portugal Email: [email protected] Abstract—In this work the effect of different maize flours usually local maize landraces are preferred [4]. Stone used for bread production was evaluated, considering their wheel mills moved by water or wind, and nowadays quality characteristics. Traditional maize breads (broas) usually by electricity, are used traditionally to obtain were produced using yellow maize and white maize, both maize flour for breadmaking. The traditional process for commercial flours and produced by local maize landraces. bread production is empirical and leads to an ethnic Flours were chemical and rheological characterized. Broas were evaluated considering the moisture, water activity (a ), product highly accepted by the consumers because of its w distinctive sensorial characteristics, mainly the flavor and hardness, color and sensorial characteristics. Moisture, aw and amylose content of flours presented similar values, with unique texture [5]. The same authors mention that, in yellow flours presenting the high value of -tocopherol (0.49 Portugal, maize flour is used in combination with rye g/g). Commercial maize flours presented high temperature flour to produce broa, a home-baked sourdough bread. and peak viscosity, with less gel stability after cooling. At the Dough for broa is obtained by mixing mayze and rye end of pasting process, the regional maize flours produced flours with water, salt, and soured via given small amount firm, stable and cuttable gels.
    [Show full text]
  • FEASIBILITY of USING ZEIN in GLUTEN-FREE BAKING By
    FEASIBILITY OF USING ZEIN IN GLUTEN-FREE BAKING by RACHEL BLOOM B.S., Kansas State University, 2008 A REPORT submitted in partial fulfillment of the requirements for the degree MASTER OF SCIENCE Food Science KANSAS STATE UNIVERSITY Manhattan, Kansas 2015 Approved by: Major Professor Karen Schmidt Copyright RACHEL BLOOM 2015 Abstract Flour is essential to bread production as it provides structure, texture, and flavor. The most common, wheat flour, is unique compared to other cereal flours as it forms gluten that is capable of forming viscoelastic dough, which retains gas produced during fermentation and helps create cohesive dough, all of which are critical to bread development. However, a certain percentage of the population has a rare autoimmune disorder, celiac disease, which is triggered by gluten. A gluten-free diet is the only remedy for celiac disease. Traditionally, in gluten-free breads, hydrocolloids, or gums have been used to mimic the behavior of gluten. However, the lack of a protein structure in breads made with hydrocolloids leads to an almost batter-like viscosity. Therefore, research has focused on gluten-free alternatives, particularly non-wheat cereal proteins that can be altered to mimic gluten’s dough forming properties. For example, zein has an average molecular weight and larger peptides than gluten, which contribute to its hydrophobic behavior. In fact, zein from maize flour is an ideal alternative as it can be manipulated to behave like gluten under certain conditions. The main difference between gluten and zein is that zein does not exhibit a large disulfide-linked polymer. Zein is also more hydrophobic than gluten.
    [Show full text]
  • Stories, Memories, Recipes: Politics of Bread As a Catalyst for Sharing in the Public Space
    ______________________________________________________________ Stories, memories, recipes: Politics of bread as a catalyst for sharing in the public space. Rayan Mario Merhy MADEP | Masters in Art and Design for the Public Space | 2018–2020 Stories, memories, recipes: Politics of bread as a catalyst for sharing in the public space. Menu Starters Introduction________________________________________________________________5 Bread on the tables of the world________________________________________________7 Mains Bread: history and its socio-political effect______________________________________11 Architecture of bread: construction of societies through bread______________________24 Transforming and mapping public experiences: sharing bread communally____________29 Desserts Conclusion________________________________________________________________34 Memory, Stories, Recipes____________________________________________________36 Bibliography ______________________________________________________________39 Drinks Catalogue of work__________________________________________________________42 1 Stories, memories, recipes: Politics of bread as a catalyst for sharing in the public space. Abstract This thesis aims to open discussion and shed light upon the importance of the exchange of intangible cultural heritage of sharing bread in social spaces. Looking at the city of Porto, Portugal, these experiences create various communication maps of people, addressing how social spaces are defined through food and the lived cultural experience that transforms
    [Show full text]
  • Sem Título De Diapositivo
    Changes in Chemical Parameters During Breadmaking of Broa as Compared with Commercial Cereals and Breads João M. Rocha & F. Xavier Malcata Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, P-4200-072 Porto, Portugal INTRODUCTION rI rV mI rXIIII - It is usual to rank Sourdoughs according to pH, 7 mIV rIV mXIII mXI rXI C A BROA is a traditional type of bread, which is manufactured in Northern T mV bV PT bXIII TTA and FQ values 6 Portugal in the absence of a starter culture at the farm level, usually following bI 5 bXI - It is expected that the Total titratable Acidity in PC2 bIV sXIII PC1 ancient protocols. This food is obtained from maize and rye flours, which is sIV sXI sI Sourdoughs increases slightly during the initial 4 sV inoculated with a given amount of previously fermented dough. Like doughs pH stage of fermentation, due the low metabolic of similar breads in other countries, the dough for BROA contains a complex 3 activity of Lactic Acid Bacteria and the high microflora which, owing to synergistic interactions, produces distinct acidic 2 activity of yeasts tastes and unique flavors; the low pH of which also contributes to extended 1 - Low pH values, as a result of fermentation by 0 Lactic Acid Bacteria, promote increases in the shelf-lives. Samples loaf volume, whereas optimal baking conditions Figure 3 - pH in samples of Maize and Rye flours, Sourdough control the enzymatic activity and elasticity will and Broa, obtained from traditional producers, as compared improving the shelf-life The consumption of this kind of sourdough bread has been increasing rapidly with commercial flours and breads.
    [Show full text]