GDF7 Polyclonal Antibody

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PRODUCT DATA SHEET Bioworld Technology,Inc. GDF7 polyclonal antibody Catalog: BS65262 Host: Rabbit Reactivity: Human,Mouse,Rat BackGround: term. Avoid freeze-thaw cycles. growth differentiation factor 7(GDF7) Homo sapiens This Specificity: gene encodes a secreted ligand of the TGF-beta (trans- GDF-7 Polyclonal Antibody detects endogenous levels of forming growth factor-beta) superfamily of proteins. GDF-7 protein. Ligands of this family bind various TGF-beta receptors DATA: leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein may play a role in the differentiation of ten- don cells and spinal cord interneurons. A mutation in this gene may be associated with increased risk for Barrett's esophagus and esophageal adenocarcinoma. [provided by Western Blot analysis of Hela cells using GDF-7 Polyclonal Antibody. RefSeq, Sep 2016] Antibody was diluted at 1:1000. Secondary antibody(catalog#：RS0002) Product: was diluted at 1:20000 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Molecular Weight: ~ 47 kDa Swiss-Prot: Q7Z4P5 Purification&Purity: Immunohistochemical analysis of paraffin-embedded human-brain, an- The antibody was affinity-purified from rabbit antiserum tibody was diluted at 1:200 by affinity-chromatography using epitope-specific im- Note: munogen. For research use only, not for use in diagnostic procedure. Applications: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELI- SA: 1/10000. Not yet tested in other applications. Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long Bioworld Technology, Inc. Bioworld technology, co. Ltd. Add： 1660 South Highway 100, Suite 500 St. Louis Park, Add： No 9, weidi road Qixia District Nanjing, 210046, MN 55416,USA. P. R. China. Email: [email protected] Email: [email protected] Tel: 6123263284 Tel: 0086-025-68037686 Fax: 6122933841 Fax: 0086-025-68035151 .

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Supplementary Data
Gene Expressional Differences in MCF-7 vs. MCF-10A cells, Venn Groups e k g b Smad target genes (48) l o n i a h m j d Transcription Factors, And Genes Regulating Developmental Processes (44) f c Adhesion and TGF Superfamily Extracellular Molecules Cytokines (27) (44) Minimum Fold expression values ->640, -320, -160, -80, -40, -20, -10, -5, -2.5, 0, 2.5, 5, 10, 20, 40, 80, 160, 320, 640< + Supplemental Fig. 1 Supplemental Table 1. Change of Basal Gene Expressional values in MCF-7 as compared to MCF-10A cell line. Fold T-test Gene # GeneBank Symbol Up/Down p-Value Description /Position Regulation Venn Group a 01 /A01 NM_001105 ACVR1 -4.96 0.0000 Activin A receptor, type I 02 /A02 NM_001616 ACVR2A -2.62 0.0007 Activin A receptor, type IIA 03 /A03 NM_000020 ACVRL1 -1.25 0.7013 Activin A receptor type II-like 1 05 /A05 NM_020547 AMHR2 1.08 0.8043 Anti-Mullerian hormone receptor, type II 16 /B04 NM_004329 BMPR1A -2.40 0.0011 Bone morphogenetic protein receptor, type IA 17 /B05 NM_001203 BMPR1B -7.37 0.0000 Bone morphogenetic protein receptor, type IB 36 /C12 NM_000557 GDF5 -1.38 0.4911 Growth differentiation factor 5 (cartilage-derived morphogenetic protein-1) 37 /D01 NM_001001557 GDF6 1.12 0.9002 Growth differentiation factor 6 38 /D02 NM_182828 GDF7 -1.52 0.4995 Growth differentiation factor 7 53 /E05 NM_020997 LEFTY1 -1.76 0.0529 Left-right determination factor 1 59 /E11 NM_018055 NODAL -3.62 0.1290 Nodal homolog (mouse) 77 /G05 NM_003238 TGFB2 -4.52 0.0566 Transforming growth factor, beta 2 78 /G06 NM_003239 TGFB3 -1.12 0.2902 Transforming
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