Unravelling the Cell Adhesion Defect in Meckel-Gruber Syndrome
Unravelling the Cell Adhesion Defect in Meckel-Gruber Syndrome Submitted by Benjamin Roland Alexander Meadows as a thesis for the degree of Doctor of Philosophy in Biological Sciences in September 2016 This thesis is available for Library use on the understanding that it is copyright material and that no quotation from the thesis may be published without proper acknowledgement. I certify that all material in this thesis which is not my own work has been identified and that no material has previously been submitted and approved for the award of a degree by this or any other University. ……………………………………………………………… 1 2 Acknowledgements The first people who must be thanked are my fellow Dawe group members Kate McIntosh, Kat Curry, and half of Holly Hardy, as well as all past group members and Helen herself, who has always been a supportive and patient supervisor with a worryingly encyclopaedic knowledge of the human proteome. Some (in the end, distressingly small) parts of this project would not have been possible without my western blot consultancy team, including senior western blot consultant Joe Costello and junior consultants Afsoon Sadeghi-Azadi, Jack Chen, Luis Godinho, Tina Schrader, Stacey Scott, Lucy Green, and Lizzy Anderson. James Wakefield is thanked for improvising a protocol for actin co- sedimentation out of almost thin air. Most surprisingly, it worked. Special thanks are due to the many undergraduates who have contributed to this project, without whose hard work many an n would be low: Beth Hickton, Grace Howells, Annie Toynbee, Alex Oldfield, Leonie Hawksley, and Georgie McDonald. Peter Splatt and Christian Hacker are thanked for their help with electron microscopy.
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