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Molecular Evolution of Numt, a Recent Transfer and Tandem Amplification of Mitochondrial DNA Into the Nuclear Genome of the Domestic Cat (Felis Catus) Jose V

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Molecular Evolution of Numt, a Recent Transfer and Tandem Amplification of Mitochondrial DNA Into the Nuclear Genome of the Domestic Cat (Felis Catus) Jose V Nova Southeastern University NSUWorks Oceanography Faculty Theses and Dissertations Department of Marine and Environmental Sciences 4-1-1995 Molecular Evolution of Numt, a Recent Transfer and Tandem Amplification of Mitochondrial DNA into the Nuclear Genome of the Domestic Cat (Felis catus) Jose V. Lopez Nova Southeastern University, [email protected] This document is a product of extensive research conducted by a faculty member at Nova Southeastern University in the Halmos College of Natural Sciences and Oceanography. For more information on faculty at the NSU Halmos College of Natural Sciences and Oceanography, please click here. Follow this and additional works at: https://nsuworks.nova.edu/occ_facetd Part of the Oceanography and Atmospheric Sciences and Meteorology Commons Share Feedback About This Item NSUWorks Citation Jose V. Lopez. 1995. Molecular Evolution of Numt, a Recent Transfer and Tandem Amplification of Mitochondrial DNA into the Nuclear Genome of the Domestic Cat (Felis catus). Doctoral dissertation. Nova Southeastern University. Retrieved from NSUWorks, Oceanographic Center. (1) https://nsuworks.nova.edu/occ_facetd/1. This Dissertation is brought to you by the Department of Marine and Environmental Sciences at NSUWorks. It has been accepted for inclusion in Oceanography Faculty Theses and Dissertations by an authorized administrator of NSUWorks. For more information, please contact [email protected]. Molecular evolution of Numt, a recent transfer and tandem ... Lopez, Jose Victor ProQuest Dissertations and Theses; 1995; ProQuest Dissertations & Theses Full Text pg. n/a INFORMATION TO USERS This mam1script.bas been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissenation copies are in typewriter face, while others may be from any type of computer printer. The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins. and impzoper alignment can adversely affect reproduction. In the unlikely. event that the author did not send UMI a complete mam1script and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. 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Molecular Evolution of Numt, a Recent Transfer and Tandem Amplification of Mitochondrial DNA into the Nuclear Genome of the Domestic Cat (Felis catus) A dissertatIon submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at George Mason University. by Jose Victor Lopez Master of Science. The Florida State University Bachelor of Science. Georgia Institute of Technology Director: Dr. Alan H. Christensen. Ph.D Department of Biology. George Mason University Spring 1995 George Mason University Fairfax. Virginia Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. OMI Number: 9528192 UMI Microform 9528192 Copyright 1995, by OMI Company. All rights reserved. This microform edition is protected against unauthorized copying under Title 17, United States Code. UMI 300 North Zeeb Road Ann Arbor, HI 48103 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. MOLECULAR EVOLUTION OF NUMT, A RECENT TRANSFER AND TANDEM AMPLIFICATION OF MITOCHONDRIAL DNA INTO THE NUCLEAR GENOME OF THE DOIVtESTIC CAT (FELIS CA TUS> by Jose Victor Lopez A Dissertation Submitted to the Graduate Faculty of George Mason University in Partial Fulfillment of the Requ iremems for the Degree of Doctor of Philosophy Environmental Biology and Public Policy --il.;::::!...!~~~"'-A-~~~-~-n- Program Director -f.;r'!l..L~~~I.J4.,(&17fr::::::Lt:.JI.~q-:' Department Chai rperson +-~~~~~~---'~~~~=?Jean of the College of AilS and Sciences Spring 1995 George Mason University Fairfax. Virginia Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. II Acknowlegements This dissertation is dedicated to my grandparents - Jose and Angela, Domingo and Victoria. Lahat mahal kita. Many people have contributed to the success of this project and my education. Although I stated similar sentiments in the preface of my Master's thesis. becoming an evolutionary geneticist has made me even more aware of my indebtedness to all who have gone before me. First and foremost, I deeply thank Dr. Stephen J. O'Brien, chief of the Laboratory ofYiral Carcinogenesis (LYC) for becoming my mentor. guiding this research and creating a professional environment which challenged and stimulated my growth as a scientist. This was an experience which I will carry with me always. I also gratefully acknowledge the contributions of Dr. Alan Christensen, chairman of my doctoral committee, for valuable, frank counsel on the technical and strategic molecular biological aspects of the project. as well as extending his biological interests into organisms with carnassial teeth. I thank Drs. Luther Brown, Harold Morowitz and Jane Flynn, other members of the doctoral committee, for lending me their time and wisdom. I feel privileged to have been able to interact closely with the talented and multi-faceted personnel of the L YC and NOAHS. The conscientious supervision and helpful advice from Dr. Naoya Yuhki in situ will always be remembered. I am grateful to Dr. William Modi, for applying his cytogenetics expertise towards solving the Nwnr problem, and Drs. Rus Hoelzel and Clay Stephens for supplementing and enhancing my Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. lit data with their erudite analyses and comments. I am also thankful for being able to share ideas and collaborations with diverse postdoctoral fellows: Drs. Ryuichi Masuda. Marilyn Raymond, Jill Pecon Slattery, Warren Johnson, Leslie Lyons, Abi Chidambarum, Lu Zhi, and Tom Laughlin. I will always cherish the fellowship and camaraderie associated with present and former graduate students of the LVC: Cheryl Winkler, Dennis Gilbert, Dianne Janczewski, Eric Brown, Sri Miththapala. Melanie Culver, Mike Cullen. Carlos Driscoll. Joelle Wentzell and Meredith Yeager. My productivity also rei ied on the smooth operation of the L VC, which tlowed from the steadfast and cheerful presences of Janice Martenson, Mary Eichelberger. and Stanley Cevario. I am indebted to the friendly assistance of both PRI and LVC front offices comprised of Amy Huter-Imming. Theresa Covell, Laraine Main, Virginia Frye. Patricia Johnson. and the late Linda Ricketts. I am also grateful to Debbie Lomb, Mark Gunnell and Gary Smythers for invaluable help with computer programs. At the NCI­ FCRDC graphics department. I am very grateful to Dru Claar-Thompson. Carolyn Whistler, Ellen Frazier. Jennifer Brown and other graphic artists for withstanding my endless figure revisions. Lastly, completion of this project would have been impossible without the care and support of my inner circle. which includes my beloved confidante. Megan James, many other Friends, my sister Stella Diffenderfer and her family, and most especially my dearest father and mother, Dr. Ernesto and Rosario Quedding Lopez. Their love and faith in my capabilities sustained me throughout the academic journey. Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. IV Table of Contents Page List of Tables ............................................................................................ vi List of Figures ......................................................................................... vii List of Abbreviations .............................................................................. ix Abstract. .............................................................................................. x Chapter 1 - Isolation and molecular characterization of Nunu from the nuclear genome of F. eatus Background ............................................................................................. 1 Objectives .............................................................................................. 9 Materials and Methcxis .............................................................................. 10 Results ................................................................................................. 17 Nuclear location of mtDNA ..................................................................... 17 Molecular cloning of nuclear and cytoplasmic mtDNA .................................. 24 Nunu as
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