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PSMA2 Rabbit mAb

Catalog No.: A9182 Recombinant

Basic Information Background

Catalog No. The is a multicatalytic proteinase complex with a highly ordered ring- A9182 shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta Observed MW subunits. are distributed throughout eukaryotic cells at a high 26KDa concentration and cleave peptides in an ATP/-dependent process in a non- lysosomal pathway. An essential function of a modified proteasome, the Calculated MW immunoproteasome, is the processing of class I MHC peptides. This encodes a 25kDa member of the peptidase T1A family, that is a 20S core alpha subunit. [provided by RefSeq, Jul 2008] Category Primary antibody

Applications WB, IHC, IF

Cross-Reactivity Human, Mouse, Rat

Recommended Dilutions Immunogen Information

WB 1:500 - 1:2000 Gene ID Swiss Prot 5683 P25787 IHC 1:50 - 1:200 Immunogen 1:50 - 1:200 IF A synthesized peptide derived from human PSMA2

Synonyms HC3; MU; PMSA2; PSC2

Contact Product Information

www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification

Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. Validation Data

Western blot analysis of extracts of various cell lines, using PSMA2 Rabbit mAb (A9182) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- embedded rat brain using PSMA2 Rabbit embedded human placenta using PSMA2 embedded mouse kidney using PSMA2 mAb (A9182) at dilution of 1:100 (40x Rabbit mAb (A9182) at dilution of 1:100 Rabbit mAb (A9182) at dilution of 1:100 lens). (40x lens). (40x lens).

Immunofluorescence analysis of HeLa cells using PSMA2 Rabbit mAb (A9182) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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