Leader in Biomolecular Solutions for Life Science PSMA2 Rabbit mAb Catalog No.: A9182 Recombinant Basic Information Background Catalog No. The proteasome is a multicatalytic proteinase complex with a highly ordered ring- A9182 shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta Observed MW subunits. Proteasomes are distributed throughout eukaryotic cells at a high 26KDa concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non- lysosomal pathway. An essential function of a modified proteasome, the Calculated MW immunoproteasome, is the processing of class I MHC peptides. This gene encodes a 25kDa member of the peptidase T1A family, that is a 20S core alpha subunit. [provided by RefSeq, Jul 2008] Category Primary antibody Applications WB, IHC, IF Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 5683 P25787 IHC 1:50 - 1:200 Immunogen 1:50 - 1:200 IF A synthesized peptide derived from human PSMA2 Synonyms HC3; MU; PMSA2; PSC2 Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using PSMA2 Rabbit mAb (A9182) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- embedded rat brain using PSMA2 Rabbit embedded human placenta using PSMA2 embedded mouse kidney using PSMA2 mAb (A9182) at dilution of 1:100 (40x Rabbit mAb (A9182) at dilution of 1:100 Rabbit mAb (A9182) at dilution of 1:100 lens). (40x lens). (40x lens). Immunofluorescence analysis of HeLa cells using PSMA2 Rabbit mAb (A9182) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..