Studies Into the Occurrence of Alpha-Onocerin in Restharrow
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A Landscape-Based Assessment of Climate Change Vulnerability for All Native Hawaiian Plants
Technical Report HCSU-044 A LANDscape-bASED ASSESSMENT OF CLIMatE CHANGE VULNEraBILITY FOR ALL NatIVE HAWAIIAN PLANts Lucas Fortini1,2, Jonathan Price3, James Jacobi2, Adam Vorsino4, Jeff Burgett1,4, Kevin Brinck5, Fred Amidon4, Steve Miller4, Sam `Ohukani`ohi`a Gon III6, Gregory Koob7, and Eben Paxton2 1 Pacific Islands Climate Change Cooperative, Honolulu, HI 96813 2 U.S. Geological Survey, Pacific Island Ecosystems Research Center, Hawaii National Park, HI 96718 3 Department of Geography & Environmental Studies, University of Hawai‘i at Hilo, Hilo, HI 96720 4 U.S. Fish & Wildlife Service —Ecological Services, Division of Climate Change and Strategic Habitat Management, Honolulu, HI 96850 5 Hawai‘i Cooperative Studies Unit, Pacific Island Ecosystems Research Center, Hawai‘i National Park, HI 96718 6 The Nature Conservancy, Hawai‘i Chapter, Honolulu, HI 96817 7 USDA Natural Resources Conservation Service, Hawaii/Pacific Islands Area State Office, Honolulu, HI 96850 Hawai‘i Cooperative Studies Unit University of Hawai‘i at Hilo 200 W. Kawili St. Hilo, HI 96720 (808) 933-0706 November 2013 This product was prepared under Cooperative Agreement CAG09AC00070 for the Pacific Island Ecosystems Research Center of the U.S. Geological Survey. Technical Report HCSU-044 A LANDSCAPE-BASED ASSESSMENT OF CLIMATE CHANGE VULNERABILITY FOR ALL NATIVE HAWAIIAN PLANTS LUCAS FORTINI1,2, JONATHAN PRICE3, JAMES JACOBI2, ADAM VORSINO4, JEFF BURGETT1,4, KEVIN BRINCK5, FRED AMIDON4, STEVE MILLER4, SAM ʽOHUKANIʽOHIʽA GON III 6, GREGORY KOOB7, AND EBEN PAXTON2 1 Pacific Islands Climate Change Cooperative, Honolulu, HI 96813 2 U.S. Geological Survey, Pacific Island Ecosystems Research Center, Hawaiʽi National Park, HI 96718 3 Department of Geography & Environmental Studies, University of Hawaiʽi at Hilo, Hilo, HI 96720 4 U. -
10 Years of Conservation in Upper Limahuli Preserve Upper Limahuli
10 years of conservation in Upper Limahuli Preserve Uma Nagendra, Merlin Edmonds, Chiemi Nagle, Zachary DeWalt, Jordan Guss (Limahuli Garden and Preserve, The National Tropical Botanical Garden, Kaua`i) Upper Limahuli Preserve The National Tropical Botanical Garden’s conservation program in Upper Limahuli Preserve is a case study in long-term ecological conservation in a remote wet montane forest. This 400-acre hanging valley contains naturally occurring populations of 254 native plant taxa, 213 of which are Hawaiian endemics. Upper Limahuli is also home to Hawaiian forest birds and breeding colonies of endangered ground-nesting seabirds `A`o (Newell’s Shearwater, Puffinus newelli) and `Ua`u (Hawaiian Petrel, Pterodroma sandwichensis). Despite the rugged topography and sheer cliffs, which provide some protection against many environmental pressures, the Preserve has changed over the roughly 25 years of management. It is vulnerable to encroachment by invasive plants such as Australian Tree Fern (Cyathea cooperi) and Himalayan Ginger (Hedychium gardnerianum) and depredation pressure by feral cats (Felis catus), rodents (Rattus spp.), and Barn Owls (Tyto alba). Natural disturbances such as Hurricane Iniki and recent landslides create more canopy openings and opportunities for further ecosystem change. In the past ten years, the conservation program in Upper Limahuli Preserve has grown from small, intermittent camping trips for invasive removal and seed collection to a multi-agency collaboration with a year-round dedicated conservation team. A variety of projects have focused on rare plant collection and propagation, invasive plant removal, and predator control. By taking advantage of new technologies and continually tailoring techniques to the needs of the landscape, the overall conservation program has advanced significantly over the course of a decade. -
The Arabidopsis KH-Domain RNA-Binding Protein ESR1 Functions in Components of Jasmonate Signalling, Unlinking Growth Restraint and Resistance to Stress
RESEARCH ARTICLE The Arabidopsis KH-Domain RNA-Binding Protein ESR1 Functions in Components of Jasmonate Signalling, Unlinking Growth Restraint and Resistance to Stress Louise F. Thatcher1*, Lars G. Kamphuis1,2, James K. Hane1¤a, Luis Oñate-Sánchez1¤b, Karam B. Singh1,2 1 CSIRO Agriculture Flagship, Centre for Environment and Life Sciences, Wembley, Western Australia, Australia, 2 The Institute of Agriculture, The University of Western Australia, Crawley, Western Australia, Australia a11111 ¤a Current address: Curtin Institute for Computation and Centre for Crop and Disease Management, Department of Environment & Agriculture, Curtin University, Bentley, Western Australia, Australia ¤b Current address: Centro de Biotecnología y Genómica de Plantas, UPM-INIA, and E.T.S.I. Agrónomos, Universidad Politécnica de Madrid, Campus de Montegancedo, Pozuelo de Alarcón, Madrid, Spain * [email protected] OPEN ACCESS Abstract Citation: Thatcher LF, Kamphuis LG, Hane JK, Oñate-Sánchez L, Singh KB (2015) The Arabidopsis Glutathione S-transferases (GSTs) play important roles in the protection of cells against tox- KH-Domain RNA-Binding Protein ESR1 Functions in ins and oxidative damage where one Arabidopsis member, GSTF8, has become a com- Components of Jasmonate Signalling, Unlinking Growth Restraint and Resistance to Stress. PLoS monly used marker gene for early stress and defense responses. A GSTF8 promoter ONE 10(5): e0126978. doi:10.1371/journal. fragment fused to the luciferase reporter gene was used in a forward genetic screen for Ara- pone.0126978 bidopsis mutants with up-regulated GSTF8 promoter activity. This identified the esr1-1 (en- Academic Editor: Ramamurthy Mahalingam, hanced stress response 1) mutant which also conferred increased resistance to the fungal Oklahoma State University, UNITED STATES pathogen Fusarium oxysporum. -
Reprogramming of Metabolism by the Arabidopsis Thaliana Bzip11 Tran- Scription Factor
Reprogramming of metabolism by the Arabidopsis thaliana bZIP11 tran- scription factor Jingkun Ma Layout, cover & invitation design: Jingkun Ma Printing: Offpage ISBN: 978-94-6182-112-6 The study presented in this thesis was performed in the group of Molecular Plant Physiology, Utrecht University, Padualaan 8, 3584CH, Utrecht, The Netherlands. The presented work was supported by Centre for BioSystems Genomics (CBSG). Reprogramming of metabolism by the Arabidopsis thaliana bZIP11 tran- scription factor Herprogrammering van het metabolisme door de Arabidopsis thaliana bZIP11 transcriptie factor (met een samenvatting in het Nederlands) Proefschrift ter verkrijging van de graad van doctor aan de Universiteit Utrecht op gezag van de rector magnificus, prof. dr. G. J. van der Zwaan, ingevolge het besluit van het college voor promoties in het openbaar te verdedigen op vrijdag 11 mei 2012 des mid- dags te 12.45 uur door Jingkun Ma geboren op 24 januari 1982 te Xiangfan, P.R.China Promoter: Prof.dr. J.C.M.Smeekens Co-promoter: Dr. S.J.Hanson CONTENTS Chapter 1 Introduction Sugar sensingChapter and signaling 3 networks 1 Chapter 2 Metabolic reprogramming mediated by bZIP11 37 ChapterProtoplast 3 The functiontranscriptomics of S1/C bZIPs in analysis gene regulation reveals the overlapping 77 and distinct functions of S1/C bZIP transcription factors in Chapter 4 The overlapping functiongene of T6Pregulation signal, SnRK1 and S1/C bZIPs 99 Chapter 5 Summarizing discussion 123 Summary in Dutch 131 AcknowledgementsJingkun Ma, Micha Hanssen, Johannes -
Putative Genes Involved in Saikosaponin Biosynthesis in Bupleurum Species
Int. J. Mol. Sci. 2013, 14, 12806-12826; doi:10.3390/ijms140612806 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 www.mdpi.com/journal/ijms Review Putative Genes Involved in Saikosaponin Biosynthesis in Bupleurum Species Tsai-Yun Lin 1,*, Chung-Yi Chiou 1 and Shu-Jiau Chiou 2 1 Institute of Bioinformatics and Structural Biology & Department of Life Science, National Tsing Hua University, No. 101, Sec. 2, Kuang Fu Rd., Hsinchu 30013, Taiwan; E-Mail: [email protected] 2 Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, No. 321, Sec. 2, Kuang Fu Rd., Hsinchu 30011, Taiwan; E-Mail: [email protected] * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +886-3-574-2758; Fax: +886-3-571-5934. Received: 22 April 2013; in revised form: 13 June 2013 / Accepted: 14 June 2013 / Published: 19 June 2013 Abstract: Alternative medicinal agents, such as the herb Bupleurum, are increasingly used in modern medicine to supplement synthetic drugs. First, we present a review of the currently known effects of triterpene saponins-saikosaponins of Bupleurum species. The putative biosynthetic pathway of saikosaponins in Bupleurum species is summarized, followed by discussions on identification and characterization of genes involved in the biosynthesis of saikosaponins. The purpose is to provide a brief review of gene extraction, functional characterization of isolated genes and assessment of expression patterns of genes encoding enzymes in the process of saikosaponin production in Bupleurum species, mainly B. kaoi. We focus on the effects of MeJA on saikosaponin production, transcription patterns of genes involved in biosynthesis and on functional depiction. -
Federal Register / Vol. 61, No. 40 / Wednesday, February 28, 1996 / Proposed Rules
7596 Federal Register / Vol. 61, No. 40 / Wednesday, February 28, 1996 / Proposed Rules DEPARTMENT OF THE INTERIOR appointment in the Regional Offices SUPPLEMENTARY INFORMATION: listed below. Fish and Wildlife Service Information relating to particular taxa Background in this notice may be obtained from the The Endangered Species Act (Act) of 50 CFR Part 17 Service's Endangered Species 1973, as amended, (16 U.S.C. 1531 et Coordinator in the lead Regional Office seq.) requires the Service to identify Endangered and Threatened Wildlife identified for each taxon and listed species of wildlife and plants that are and Plants; Review of Plant and below: endangered or threatened, based on the Animal Taxa That Are Candidates for Region 1. California, Commonwealth best available scientific and commercial Listing as Endangered or Threatened of the Northern Mariana Islands, information. As part of the program to Species Hawaii, Idaho, Nevada, Oregon, Pacific accomplish this, the Service has AGENCY: Fish and Wildlife Service, Territories of the United States, and maintained a list of species regarded as Interior. Washington. candidates for listing. The Service maintains this list for a variety of ACTION: Notice of review. Regional Director (TE), U.S. Fish and Wildlife Service, Eastside Federal reasons, includingÐto provide advance SUMMARY: In this notice the Fish and Complex, 911 N.E. 11th Avenue, knowledge of potential listings that Wildlife Service (Service) presents an Portland, Oregon 97232±4181 (503± could affect decisions of environmental updated list of plant and animal taxa 231±6131). planners and developers; to solicit input native to the United States that are Region 2. -
Syntenic Gene and Genome Duplication Drives Diversification of Plant Secondary Metabolism and Innate Immunity in Flowering Plants
Genomics 4.0 - Syntenic Gene and Genome Duplication Drives Diversification of Plant Secondary Metabolism and Innate Immunity in Flowering Plants - Advanced Pattern Analytics in Duplicate Genomes - Johannes A. Hofberger Thesis committee Promotor Prof. Dr M. Eric Schranz Professor of Experimental Biosystematics Wageningen University Other members Prof. Dr Bart P.H.J. Thomma, Wageningen University Prof. Dr Berend Snel, Utrecht University Dr Klaas Vrieling, Leiden University Dr Gabino F. Sanchez, Wageningen University This research was conducted under the auspices of the Graduate School of Experimental Plant Sciences. Genomics 4.0 - Syntenic Gene and Genome Duplication Drives Diversification of Plant Secondary Metabolism and Innate Immunity in Flowering Plants - Advanced Pattern Analytics in Duplicate Genomes - Johannes A. Hofberger Thesis submitted in fulfilment of the requirements for the degree of doctor at Wageningen University by the authority of the Rector Magnificus Prof. Dr M.J. Kropff, in the presence of the Thesis Committee appointed by the Academic Board to be defended in public on Monday 18 May 2015 at 4 p.m. in the Aula. Johannes A. Hofberger Genomics 4.0 - Syntenic Gene and Genome Duplication Drives Diversification of Plant Secondary Metabolism and Innate Immunity in Flowering Plants 83 pages. PhD thesis, Wageningen University, Wageningen, NL (2015) With references, with summaries in Dutch and English ISBN: 978-94-6257-314-7 PROPOSITIONS 1. Ohnolog over-retention following ancient polyploidy facilitated diversification of the glucosinolate biosynthetic inventory in the mustard family. (this thesis) 2. Resistance protein conserved in structurally stable parts of plant genomes confer pleiotropic effects and expanded functions in plant innate immunity. (this thesis) 3. -
Influences of Gravitational Intensity on the Transcriptional Landscape of Arabidopsis
Influences of Gravitational Intensity on the Transcriptional Landscape of Arabidopsis thaliana A dissertation presented to the faculty of the College of Arts and Sciences of Ohio University In partial fulfillment of the requirements for the degree Doctor of Philosophy Alexander D. Meyers May 2020 © 2020 Alexander D. Meyers. All Rights Reserved. 2 This dissertation titled Influences of Gravitational Intensity on the Transcriptional Landscape of Arabidopsis thaliana by ALEXANDER D. MEYERS has been approved for the Department of Molecular and Cellular Biology and the College of Arts and Sciences by Sarah E. Wyatt Professor of Environmental and Plant Biology Florenz Plassmann Dean, College of Arts and Sciences 3 Abstract MEYERS, ALEXANDER D, Ph.D., May 2020, Molecular and Cellular Biology Influences of Gravitational Intensity on the Transcriptional Landscape of Arabidopsis thaliana Director of Dissertation: Sarah E. Wyatt Plants use a myriad of environmental cues to inform their growth and development. The force of gravity has been a consistent abiotic input throughout plant evolution, and plants utilize gravity sensing mechanisms to maintain proper orientation and architecture. Despite thorough study, the specific mechanics behind plant gravity perception remain largely undefined or unproven. At the center of plant gravitropism are dense, specialized organelles called starch statoliths that sediment in the direction of gravity. Herein I describe a series of experiments in Arabidopsis that leveraged RNA sequencing to probe gravity response mechanisms in plants, utilizing reorientation in Earth’s 1g, fractional gravity environments aboard the International Space Station, and simulated fractional and hyper gravity environments within various specialized hardware. Seedlings were examined at organ-level resolution, and the statolith-deficient pgm-1 mutant was subjected to all treatments alongside wildtype seedlings in an effort to resolve the impact of starch statoliths on gravity response. -
Mortierellaceae Phylogenomics and Tripartite Plant-Fungal-Bacterial Symbiosis of Mortierella Elongata
MORTIERELLACEAE PHYLOGENOMICS AND TRIPARTITE PLANT-FUNGAL-BACTERIAL SYMBIOSIS OF MORTIERELLA ELONGATA By Natalie Vandepol A DISSERTATION Submitted to Michigan State University in partial fulfillment of the requirements for the degree of Microbiology & Molecular Genetics – Doctor of Philosophy 2020 ABSTRACT MORTIERELLACEAE PHYLOGENOMICS AND TRIPARTITE PLANT-FUNGAL-BACTERIAL SYMBIOSIS OF MORTIERELLA ELONGATA By Natalie Vandepol Microbial promotion of plant growth has great potential to improve agricultural yields and protect plants against pathogens and/or abiotic stresses. Soil fungi in Mortierellaceae are non- mycorrhizal plant associates that frequently harbor bacterial endosymbionts. My research focused on resolving the Mortierellaceae phylogeny and on characterizing the effect of Mortierella elongata and its bacterial symbionts on Arabidopsis thaliana growth and molecular functioning. Early efforts to classify Mortierellaceae were based on morphology, but phylogenetic studies with ribosomal DNA (rDNA) markers have demonstrated conflicting taxonomic groupings and polyphyletic genera. In this study, I applied two approaches: low coverage genome (LCG) sequencing and high-throughput targeted amplicon sequencing to generate multi-locus sequence data. I combined these datasets to generate a well-supported genome-based phylogeny having broad sampling depth from the amplicon dataset. Resolving the Mortierellaceae phylogeny into monophyletic groups led to the definition of 14 genera, 7 of which are newly proposed. Mortierellaceae are broadly considered plant associates, but the underlying mechanisms of association are not well understood. In this study, I focused on the symbiosis between M. elongata, its endobacteria, and A. thaliana. I measured aerial plant growth and seed production and used transcriptomics to characterize differentially expressed plant genes (DEGs) while varying fungal treatments. M. elongata was shown to promote aerial plant growth and affect seed production independent of endobacteria. -
Proquest Dissertations
RICE UNIVERSITY Investigation of Triterpene Biosynthesis in Arabidopsis thaliana by Mariya D. Kolesnikova A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE Doctor of Philosophy APPROVED, THESIS COMMITTEE: Seircni P. T. Matsuda, Professor, Department Chair Department of Chemistry Department of Biochemistry and Cell Biology JU- Ronald J. Parry, Professor Department of Chemistry Department of Biochemistry and Cell Biology UL Jonatnan Silberg, ^gs&tant Profasabr Department of Biochemistry and Cell Biology HOUSTON, TEXAS May 2008 UMI Number: 3362344 INFORMATION TO USERS The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleed-through, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. UMI® UMI Microform 3362344 Copyright 2009 by ProQuest LLC All rights reserved. This microform edition is protected against unauthorized copying under Title 17, United States Code. ProQuest LLC 789 East Eisenhower Parkway P.O. Box 1346 Ann Arbor, Ml 48106-1346 ii ABSTRACT Investigation of Triterpene Biosynthesis in Arabidopsis thaliana By Mariya D. Kolesnikova This thesis describes functional characterization of three oxidosqualene cyclase genes (Atlg78955, At3g45130, and At4gl5340) from the model plant Arabidopsis thaliana that encode enzymes with novel catalytic functions. Oxidosqualene cyclases are a family of membrane proteins that convert the acyclic substrate oxidosqualene into polycyclic products with many chiral centers. The complex mechanistic pathways and relevant catalytic motifs can be elucidated through judicious applications of mutagenesis, heterologous expression in combination with a genome mining approach, and protein modeling. -
The Use of Mutants and Inhibitors to Study Sterol Biosynthesis in Plants
bioRxiv preprint doi: https://doi.org/10.1101/784272; this version posted September 26, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Title page 2 Title: The use of mutants and inhibitors to study sterol 3 biosynthesis in plants 4 5 Authors: Kjell De Vriese1,2, Jacob Pollier1,2,3, Alain Goossens1,2, Tom Beeckman1,2, Steffen 6 Vanneste1,2,4,* 7 Affiliations: 8 1: Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, 9 Belgium 10 2: VIB Center for Plant Systems Biology, VIB, Technologiepark 71, 9052 Ghent, Belgium 11 3: VIB Metabolomics Core, Technologiepark 71, 9052 Ghent, Belgium 12 4: Lab of Plant Growth Analysis, Ghent University Global Campus, Songdomunhwa-Ro, 119, Yeonsu-gu, Incheon 13 21985, Republic of Korea 14 15 e-mails: 16 K.D.V: [email protected] 17 J.P: [email protected] 18 A.G. [email protected] 19 T.B. [email protected] 20 S.V. [email protected] 21 22 *Corresponding author 23 Tel: +32 9 33 13844 24 Date of submission: sept 26th 2019 25 Number of Figures:3 in colour 26 Word count: 6126 27 28 1 bioRxiv preprint doi: https://doi.org/10.1101/784272; this version posted September 26, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. -
Possible Extinctions, Rediscoveries, and New Plant Records Within The
Records of the Hawaii Biological Survey for 2011. Edited by 91 Neal L. Evenhuis & Lucius G. Eldredge. Bishop Museum Occasional Papers 113: 91 –102 (2012) Possible Extinctions , Rediscoveries, and New Plant Records within the Hawaiian Islands 1 KeNNetH R. W ooD 2 National Tropical Botanical Garden, 3530 Papalina Road, Kalaheo, Kaua‘i, Hawai‘i 96741, USA; email: [email protected] eleven possible new extinctions are reported for the Hawaiian flora, in addition to 5 island records, 3 range rediscoveries, 1 rediscovery, and 1 new naturalized record. the remark - able range rediscoveries of Ctenitis squamigera (Dryopteridaceae) and Lysimachia filifo - lia (Primulaceae) give hope toward their future conservation, as both are federally listed as endangered and were undocumented on Kaua‘i for ca 100 years. Yet there is great con - cern over numerous possible plant extinctions in Hawai‘i. two extinctions were recently reported from Kaua‘i (i.e., Dubautia kenwoodii and Cyanea kuhihewa ) (Wood 2007), and an additional 11 are now reported to have no known living individuals in the wild. Species abundance will naturally fluctuate, yet for very rare taxa there is little room for decline. the ongoing decline of native pollinators (Kearns et al. 1998) and seed dispersers (Mil - berg & tyrberg 1993), in combination with other primary extrinsic factors such as inva - sive nonnative plants, predation by introduced vertebrates, loss and fragmentation of nat - ural habitats, and devastation by severe storms, are leading to an increase in extinctions throughout the islands of oceania (Sakai et al. 2002; Wood 2007; Kingsford et al. 2009). the assertion of extinction is potentially fallible and can only be inferred from absence of sighting or collection records (Solow & Roberts 2003).