Isolation and Functional Characterization of CYP71AJ4
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
Synthesis, Characterization and Spectroscopic Properties of Phthalocyanines Bearing Umbelliferone Moieties
Çamur&Bulut/ Kirklareli University Journal of Engineering and Science 2 (2016) 120-128 SYNTHESIS, CHARACTERIZATION AND SPECTROSCOPIC PROPERTIES OF PHTHALOCYANINES BEARING UMBELLIFERONE MOIETIES Meryem ÇAMUR1*, Mustafa BULUT2 1Kırklareli University, Chemistry, 39100, Kırklareli, Turkey 2Marmara University, Chemistry, 34722 Göztepe-Istanbul, Turkey Abstract The preparing and structure determination of new phthalonitrile complex bearing umbelliferone substituent and its phthalocyanine derivatives [M= metal-free, zinc (II), cobalt (II), copper (II)] were made. The structures of these original complexes were characterized by infrared, proton nuclear magnetic resonance, ultraviolet-visible and mass spectroscopic methods. Keywords: Phthalocyanine; Coumarin; Spectroscopy. UMBELLIFERON GRUPLARI İÇEREN FTALOSİYANİNLERİN SENTEZİ, KARAKTERİZASYONU ve SPEKTROSKOPİK ÖZELLİKLERİNİN İNCELENMESİ Özet Bu çalışmada umbelliferon sübstitüe ftalonitril bileşiği ve non-periferal olarak sübstitüe metalsiz, çinko (II), kobalt (II), bakır (II) metalli ftalosiyanin türevleri ilk kez sentezlenerek yapıları aydınlatılmıştır. Bu orjinal bileşiklerin yapıları infrared, proton nükleer magnetik rezonans, ultraviyole-görünür bölge ve kütle spektroskopisi gibi spektroskopik metodlardan elde edilen verilerle tayin edilmiştir. Anahtar Kelimeler: Ftalosiyanin, Kumarin, Spektroskopi. *Correspondence to: Tel.: +90-288-2461734 / 3514; fax: +90-288-2461733; E-mail addresses: [email protected], [email protected] Synthesis, Characterization and Spectroscopic Properties -
1,1,1,2-Tetrafluoroethane
This report contains the collective views of an international group of experts and does not necessarily represent the decisions or the stated policy of the United Nations Environment Programme, the International Labour Organisation, or the World Health Organization. Concise International Chemical Assessment Document 11 1,1,1,2-Tetrafluoroethane First draft prepared by Mrs P. Barker and Mr R. Cary, Health and Safety Executive, Liverpool, United Kingdom, and Dr S. Dobson, Institute of Terrestrial Ecology, Huntingdon, United Kingdom Please not that the layout and pagination of this pdf file are not identical to the printed CICAD Published under the joint sponsorship of the United Nations Environment Programme, the International Labour Organisation, and the World Health Organization, and produced within the framework of the Inter-Organization Programme for the Sound Management of Chemicals. World Health Organization Geneva, 1998 The International Programme on Chemical Safety (IPCS), established in 1980, is a joint venture of the United Nations Environment Programme (UNEP), the International Labour Organisation (ILO), and the World Health Organization (WHO). The overall objectives of the IPCS are to establish the scientific basis for assessment of the risk to human health and the environment from exposure to chemicals, through international peer review processes, as a prerequisite for the promotion of chemical safety, and to provide technical assistance in strengthening national capacities for the sound management of chemicals. The Inter-Organization -
Suspect and Target Screening of Natural Toxins in the Ter River Catchment Area in NE Spain and Prioritisation by Their Toxicity
toxins Article Suspect and Target Screening of Natural Toxins in the Ter River Catchment Area in NE Spain and Prioritisation by Their Toxicity Massimo Picardo 1 , Oscar Núñez 2,3 and Marinella Farré 1,* 1 Department of Environmental Chemistry, IDAEA-CSIC, 08034 Barcelona, Spain; [email protected] 2 Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, 08034 Barcelona, Spain; [email protected] 3 Serra Húnter Professor, Generalitat de Catalunya, 08034 Barcelona, Spain * Correspondence: [email protected] Received: 5 October 2020; Accepted: 26 November 2020; Published: 28 November 2020 Abstract: This study presents the application of a suspect screening approach to screen a wide range of natural toxins, including mycotoxins, bacterial toxins, and plant toxins, in surface waters. The method is based on a generic solid-phase extraction procedure, using three sorbent phases in two cartridges that are connected in series, hence covering a wide range of polarities, followed by liquid chromatography coupled to high-resolution mass spectrometry. The acquisition was performed in the full-scan and data-dependent modes while working under positive and negative ionisation conditions. This method was applied in order to assess the natural toxins in the Ter River water reservoirs, which are used to produce drinking water for Barcelona city (Spain). The study was carried out during a period of seven months, covering the expected prior, during, and post-peak blooming periods of the natural toxins. Fifty-three (53) compounds were tentatively identified, and nine of these were confirmed and quantified. Phytotoxins were identified as the most frequent group of natural toxins in the water, particularly the alkaloids group. -
The Limitations of DNA Interstrand Cross-Link Repair in Escherichia Coli
Portland State University PDXScholar Dissertations and Theses Dissertations and Theses 7-12-2018 The Limitations of DNA Interstrand Cross-link Repair in Escherichia coli Jessica Michelle Cole Portland State University Follow this and additional works at: https://pdxscholar.library.pdx.edu/open_access_etds Part of the Biology Commons Let us know how access to this document benefits ou.y Recommended Citation Cole, Jessica Michelle, "The Limitations of DNA Interstrand Cross-link Repair in Escherichia coli" (2018). Dissertations and Theses. Paper 4489. https://doi.org/10.15760/etd.6373 This Thesis is brought to you for free and open access. It has been accepted for inclusion in Dissertations and Theses by an authorized administrator of PDXScholar. Please contact us if we can make this document more accessible: [email protected]. The Limitations of DNA Interstrand Cross-link Repair in Escherichia coli by Jessica Michelle Cole A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Biology Thesis Committee: Justin Courcelle, Chair Jeffrey Singer Rahul Raghavan Portland State University 2018 i Abstract DNA interstrand cross-links are a form of genomic damage that cause a block to replication and transcription of DNA in cells and cause lethality if unrepaired. Chemical agents that induce cross-links are particularly effective at inactivating rapidly dividing cells and, because of this, have been used to treat hyperproliferative skin disorders such as psoriasis as well as a variety of cancers. However, evidence for the removal of cross- links from DNA as well as resistance to cross-link-based chemotherapy suggests the existence of a cellular repair mechanism. -
Phototoxicity of 7-Oxycoumarins with Keratinocytes in Culture T ⁎ Christophe Guillona, , Yi-Hua Janb, Diane E
Bioorganic Chemistry 89 (2019) 103014 Contents lists available at ScienceDirect Bioorganic Chemistry journal homepage: www.elsevier.com/locate/bioorg Phototoxicity of 7-oxycoumarins with keratinocytes in culture T ⁎ Christophe Guillona, , Yi-Hua Janb, Diane E. Heckc, Thomas M. Marianob, Robert D. Rappa, Michele Jettera, Keith Kardosa, Marilyn Whittemored, Eric Akyeaa, Ivan Jabine, Jeffrey D. Laskinb, Ned D. Heindela a Department of Chemistry, Lehigh University, Bethlehem, PA 18015, USA b Department of Environmental and Occupational Health, Rutgers University School of Public Health, Piscataway, NJ 08854, USA c Department of Environmental Science, New York Medical College, Valhalla, NY 10595, USA d Buckman Laboratories, 1256 N. McLean Blvd, Memphis, TN 38108, USA e Laboratoire de Chimie Organique, Université Libre de Bruxelles, B-1050 Brussels, Belgium ARTICLE INFO ABSTRACT Keywords: Seventy-one 7-oxycoumarins, 66 synthesized and 5 commercially sourced, were tested for their ability to inhibit 7-hydroxycoumarins growth in murine PAM212 keratinocytes. Forty-nine compounds from the library demonstrated light-induced 7-oxycoumarins lethality. None was toxic in the absence of UVA light. Structure-activity correlations indicate that the ability of Furocoumarins the compounds to inhibit cell growth was dependent not only on their physiochemical characteristics, but also Psoralens on their ability to absorb UVA light. Relative lipophilicity was an important factor as was electron density in the Methoxsalen pyrone ring. Coumarins with electron withdrawing moieties – cyano and fluoro at C – were considerably less 8-MOP 3 Phototoxicity active while those with bromines or iodine at that location displayed enhanced activity. Coumarins that were PAM212 keratinocytes found to inhibit keratinocyte growth were also tested for photo-induced DNA plasmid nicking. -
Functionalization of Heterocycles: a Metal Catalyzed Approach Via
FUNCTIONALIZATION OF HETEROCYCLES: A METAL CATALYZED APPROACH VIA ALLYLATION AND C-H ACTIVATION A Dissertation Submitted to the Graduate Faculty of the North Dakota State University of Agriculture and Applied Science By Sandeepreddy Vemula In Partial Fulfillment of the Requirements for the Degree of DOCTOR OF PHILOSOPHY Major Department: Chemistry and Biochemistry October 2018 Fargo, North Dakota North Dakota State University Graduate School Title FUNCTIONALIZATION OF HETEROCYCLES: A METAL CATALYZED APPROACH VIA ALLYLATION AND C-H ACTIVATION By Sandeepreddy Vemula The Supervisory Committee certifies that this disquisition complies with North Dakota State University’s regulations and meets the accepted standards for the degree of DOCTOR OF PHILOSOPHY SUPERVISORY COMMITTEE: Prof. Gregory R. Cook Chair Prof. Mukund P. Sibi Prof. Pinjing Zhao Prof. Dean Webster Approved: November 16, 2018 Prof. Gregory R. Cook Date Department Chair ABSTRACT The central core of many biologically active natural products and pharmaceuticals contain N-heterocycles, the installation of simple/complex functional groups using C-H/N-H functionalization methodologies has the potential to dramatically increase the efficiency of synthesis with respect to resources, time and overall steps to key intermediate/products. Transition metal-catalyzed functionalization of N-heterocycles proved as a powerful tool for the construction of C-C and C-heteroatom bonds. The work in this dissertation describes the development of palladium catalyzed allylation, and the transition metal catalyzed C-H activation for selective functionalization of electron deficient N-heterocycles. Chapter 1 A thorough study highlighting the important developments made in transition metal catalyzed approaches for C-C and C-X bond forming reactions is discussed with a focus on allylation, directed indole C-2 substitution and vinylic C-H activation. -
Antithrombotic Treatment After Stroke Due to Intracerebral Haemorrhage (Review)
Cochrane Database of Systematic Reviews Antithrombotic treatment after stroke due to intracerebral haemorrhage (Review) Perry LA, Berge E, Bowditch J, Forfang E, Rønning OM, Hankey GJ, Villanueva E, Al-Shahi Salman R Perry LA, Berge E, Bowditch J, Forfang E, Rønning OM, Hankey GJ, Villanueva E, Al-Shahi Salman R. Antithrombotic treatment after stroke due to intracerebral haemorrhage. Cochrane Database of Systematic Reviews 2017, Issue 5. Art. No.: CD012144. DOI: 10.1002/14651858.CD012144.pub2. www.cochranelibrary.com Antithrombotic treatment after stroke due to intracerebral haemorrhage (Review) Copyright © 2017 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd. TABLE OF CONTENTS HEADER....................................... 1 ABSTRACT ...................................... 1 PLAINLANGUAGESUMMARY . 2 SUMMARY OF FINDINGS FOR THE MAIN COMPARISON . ..... 3 BACKGROUND .................................... 5 OBJECTIVES ..................................... 5 METHODS ...................................... 6 RESULTS....................................... 8 Figure1. ..................................... 9 Figure2. ..................................... 11 Figure3. ..................................... 12 DISCUSSION ..................................... 14 AUTHORS’CONCLUSIONS . 15 ACKNOWLEDGEMENTS . 15 REFERENCES ..................................... 15 CHARACTERISTICSOFSTUDIES . 18 DATAANDANALYSES. 31 Analysis 1.2. Comparison 1 Short-term antithrombotic treatment, Outcome 2 Death. 31 Analysis 1.6. Comparison 1 Short-term antithrombotic -
Prepared by 3M
DRAFT ECOTOXICOLOGY AND ENVIRONMENTAL FATE TESTING OF SHORT CHAIN PERFLUOROALKYL COMPOUNDS RELATED TO 3M CHEMISTRIES XXX XX, 2008 Prepared by 3M Page 1 CONFIDENTIAL - SUBJECT TO A PROTECTIVE ORDER ENTERED IN 3M MN01537089 HENNEPIN COUNTY DISTRICT COURT, NO. 27-CV-10-28862 2231.0001 DRAFT TABLE OF CONTENTS Page Introduction (describes propose, goals, document organization .... ) 3 1) Degradation modes 4-27 Basic degradation pathways, Conversion routes from polymer to monomer & degradants or residual intermediates to degradants) 2) Degradants & Intermediates - Phys. Chem properties and test data by section & chapter i) Fluorinated Sulfonic acids and derivatives a) PFBS (PBSK) 28-42 b) PFBSI 43-44 ii) Fluorinated Carboxylates a) TFA 46-55 b) PFPA 56-59 c) PFBA (APFB) 60-65 d) MeFBSAA (MeFBSE Acid, M370) 66-68 iii) Fluorinated Non-ionics a) MeFBSE 70-74 b) MeFBSA 75-80 c) FBSE 81-84 d) FBSA 85-88 e) HxFBSA 89-91 iv) Fluorinated Inerts & volatiles a) PBSF 94-97 b) NFB, C4 hydride, CF3CF2CF2CF2-H 98-99 3) Assessments X Glossary 100-102 References/Endnotes 105- Page 2 CONFIDENTIAL - SUBJECT TO A PROTECTIVE ORDER ENTERED IN 3M MN01537090 HENNEPIN COUNTY DISTRICT COURT, NO. 27-CV-10-28862 2231.0002 DRAFT Introduction to Environmental White Papers on Perfluoroalkyl acids related to 3M chemistries Perfluorochemicals have been commonplace in chemical industry over 50 years but until recently there has been little information on environmental fate and effects avialble in open literature. The following chapters summarize the findings of"list specific C4 intermediates PFBS, PFBSI, PFBA, PFPA, MeFBSAA, TFA, MeFBSE, FBSA, HxFBSA, FBSE, PBSF, NFB " As background, 3M announced on May 16, 2000 the voluntary manufacturing phase out of perfluorooctanyl chemicals which included perfluorooctanoic acid (PFOA), perfluorooctane sulfonate (PFOS), and PFOS-related chemistries. -
Natural Compounds, Fraxin and Chemicals Structurally Related to Fraxin Protect Cells from Oxidative Stress
EXPERIMENTAL and MOLECULAR MEDICINE, Vol. 37, No. 5, 436-446, October 2005 Natural compounds, fraxin and chemicals structurally related to fraxin protect cells from oxidative stress Wan Kyunn Whang1, Hyung Soon Park2, genes expressed differentially by fraxin and to InHye Ham1, Mihyun Oh1, compare antioxidative effect of fraxin with its struc- Hong Namkoong3, Hyun Kee Kim3, turally related chemicals. Of the coumarins, protective 2 4 effects of fraxin against cytotoxicity induced by H2O2 Dong Whi Hwang , Soo Young Hur , were examined in human umbilical vein endothelial 4 5 Tae Eung Kim , Yong Gyu Park , cells (HUVECs). Fraxin showed free radical scavenging Jae-Ryong Kim6 and Jin Woo Kim3,4,7 effect at high concentration (0.5 mM) and cell protective effect against H2O2-mediated oxidative stress. Fraxin 1 College of Pharmacy, Chung-Ang University recovered viability of HUVECs damaged by H2O2- 221, Heukseok-dong, Dongjak-gu treatment and reduced the lipid peroxidation and the Seoul 156-861, Korea internal reactive oxygen species level elevated by H2O2 2KeyGene Life Science Institute treatment. Differential display reverse transcrip- KeyGene Science, Corp. tion-PCR revealed that fraxin upregulated antiapo- Ansan, Gyeonggido 425-791, Korea ptotic genes (clusterin and apoptosis inhibitor 5) and 3Molecular Genetic Laboratory tumor suppressor gene (ST13). Based on structural Research Institute of Medical Science similarity comparing with fraxin, seven chemicals, 4Department of Obstetrics and Gynecology fraxidin methyl ether (29.4% enhancement of viability), 5Department of Biostatistics prenyletin (26.4%), methoxsalen (20.8 %), diffratic acid College of Medicine (19.9%), rutoside (19.1%), xanthyletin (18.4%), and The Catholic University of Korea kuhlmannin (18.2%), enhanced more potent cell via- Seoul 137-040, Korea bility in the order in comparison with fraxin, which 6Department of Biochemistry and Molecular Biology showed only 9.3% enhancement of cell viability. -
Esculetin) - an Antirheumatoid Arthritic Compound: an Update
Online - 2455-3891 Vol 11, Special issue 4, 2018 Print - 0974-2441 Research Article COUMARIN (ESCULETIN) - AN ANTIRHEUMATOID ARTHRITIC COMPOUND: AN UPDATE JAYA KUMARI S*, ANANDHI N, MOUNISHA B, MOHAMED SAMEER MH Department of Pharmacognosy, School of Pharmaceutical Sciences, Vels Institute of Science Technology and Advanced Studies, Pallavaram, Chennai, Tamil Nadu, India. Email: [email protected] Received: 11 October 2018, Revised and Accepted: 15 December 2018 ABSTRACT Context: Esculetin is a natural polyphenolic compound. It is chemically 6,7-dihydroxycoumarin and one of the ingredients of Cortex fraxini, a Chinese traditional medicine. It is used as a dietary supplement and found as non-toxic. Recently, there are many research works evaluated on esculetin in arthritis with supported molecular mechanisms. Objectives: Esculetin becoming more attractive prodrug for arthritis. Hence, the present minireview will consolidate the targeted site of esculetin in the treatment of arthritis over the past decade. Results: The most important molecular mechanism of esculetin is an antioxidant activities with decreased level of reactive oxygen species/reactive nitrogen species. It also inhibited lipoxygenase 5, lipoxygenase 12, and tyrosinase enzymes. It reduces the inflammation by modulating the key inflammatory enzyme matrix metalloproteinase-1 activity. It also lowers the nitrous oxide and prostaglandin E2 level in synovial fluid. Esculetin derivatives such as 5-methoxy esculetin inhibited the activity of nitrogen-activated protein kinases. The updated data also reveal that esculetin suppresses the leukotriene B4 level in plasma of adjuvant-induced arthritis tested animals. Conclusion: The presented update showed that esculetin may be useful as a tool in regulating the mechanism and physiological functions of the inflammatory mediators and enzyme. -
Biosynthesis of Daphnetin in Daphne Mezereum L.*
Biosynthesis of Daphnetin in Daphne mezereum L.* Stewart A. Brown Department of Chemistry, Trent University, Peterborough, Ont., Canada K9J 7B8 Z. Naturforsch. 41c, 247—252 (1986); received November 4, 1985 Biosynthesis, Coumarins, Daphne mezereum, Daphnetin Shoots of Daphne mezereum synthesized daphnetin (7,8-dihydroxycoumarin) more efficiently from [2-l4C]umbelliferone (7-hydroxycoumarin) than from [2-l4C]p-coumaric acid, and [2-14C]caf- feic acid was more poorly utilized still. These findings do not support the idea of derivation of daphnetin via hydroxylation of the caffeic acid ring at the 2 position, followed by lactone ring formation; instead they are consistent with the concept of daphnetin formation by an additional hydroxylation of umbelliferone at C-8. Umbelliferone was recovered with little l4C dilution from emulsin-hydrolysed extracts of shoots fed labelled umbelliferone, and TLC of extracts from un treated shoots revealed two substances yielding umbelliferone on hydrolysis. Evidence is pre sented from TLC and HPLC analysis that one of these is skimmin (7-O-ß-D-glucosylumbel- liferone), not previously reported from Daphne. The tracer experiments further support the theory that umbelliferone is the general precursor of coumarins bearing two or more hydroxyl functions on the aromatic ring. Introduction (Thymelaeaceae), a hardy perennial shrub, where it Approximately 70 coumarins with the 7,8 oxygen occurs together with its 7-ß-D-glucoside, daphnin ation pattern' have been reported to be naturally (6b) and daphnetin glucoside (6c), the 8-O-ß-D- occurring [1], over half being furanocoumarins de g lu c o s y la te d is o m e r [1]. -
And Citrus Fruits
Journal of the Science of Food and Agriculture J Sci Food Agric 87:2152–2163 (2007) Analysis of furanocoumarins in vegetables (Apiaceae) and citrus fruits (Rutaceae) Radek Peroutka, Veraˇ Schulzova,´ ∗ Petr Botek and Jana Hajslovˇ a´ Institute of Chemical Technology, Department of Food Chemistry and Analysis, Technicka´ 3, 166 28 Prague 6, Czech Republic Abstract: Several alternative approaches applicable for the analysis of furanocoumarins, toxic components occurring in some fruits and vegetables representing both Apiaceae and Rutaceae families, were tested in our study. Limits of detection (LODs) for angelicin, psoralen, bergapten, xanthotoxin, trioxsalen, isopimpinellin, sphondin, pimpinellin and isobergapten obtained by GC/MS (SIM) were in the range 0.01–0.08 µgg−1.Slightly higher LODs (0.02–0.20 µgg−1) were achieved by LC/MS–MS. The latter is the only alternative for analysis of bergamottin (LOD = 0.01 µgg−1) in citrus fruits because this furanocoumarin is unstable under GC conditions. Regardless of the determination step used, the repeatability of the measurements (expressed as RSD) did not exceed 10%. As shown in our study the levels of furanocoumarins in celery, celeriac, parsnip, carrot, lemon and other foods obtained at a retail market varied over a wide range; the highest contents were determined in parsnip, while the levels of these toxins in carrots and citrus pulps were relatively low. 2007 Society of Chemical Industry Keywords: furanocoumarins; GC/MS; LC/MS–MS; fruits; vegetables INTRODUCTION and fruit of some of these, e.g. figs, representing the Furanocoumarins are toxic secondary metabolites that last family, is also used for human consumption.