National Sanitary Veterinary and Food Safety Authority of Romania

Information on Newcastle Disease in Romania during 2007 SCFCAH, Brussels 03-04th of December 2007 Summary

Newcastle disease cases during 2007 Map of cases during 2007 Causes of the diseases Program for surveillance of Newcastle disease Flux diagram for virological and serological tests Laboratory testing in 2007 Newcastle disease cases during 2007

† Disease evolved only in non professional system. † 24 outbreaks, in poultry, in 10 counties; † Only one outbreak active at the moment, in Covasna County, Comanda locality; this follows to be closed on 03th of December 2007

Causes of the disease

† Poor or lack of bio-safety measures in backyard flocks † Contact with wild species † Poor immunization or backyard flocks or flocks which escaped to the immunization activities, even if vaccination is compulsory on Romanian territory Program for surveillance of Newcastle disease

Surveillance of flocks on Romanian territory is made in accordance with the provisions of: - Council Directive 92/66/EC - Commission Decision 94/327/EC - Both transposed into Romanian legislation FLUX DIAGRAM Virological tests for A160

CloacalCloacal and and tracheal tracheal swabsswabs and and tissues tissues

VirologicalVirological exam exam

HA+HA+ HA-HA-

IdentificationIdentification with with specificspecific antiserum antiserum for for NDVNDV and and AI AI

PositivePositive NDV NDV NegativeNegative IA IA

ICPIICPI SequenceSequence Serological tests for A160

SerumSerum Samples Samples CollectedCollected from from suspected suspected poultry poultry fromfrom professional professional and and non non professional professional holdings holdings

HIHI testtest forfor NDVNDV

POSITIVEPOSITIVE NEGATIVENEGATIVE DifferentDifferent titers titers of of antibodies antibodies follows the lab investigations (>1/5(>1/5 12) 12) follows the lab investigations

VirologicVirologic Exam Exam Testing in 2007 (up to 31-th of October)

† Through virological exams: - 8739 samples - 159 positive samples

Through serological exams (Haemagglutination inhibition test); - 109818 samples; - 538 samples positive Positive samples are sent to the CRL for AI and ND Thank you for listening! Paramyxovirus in pigeons in Belgium

FASFC – DG Control Policy

SCOFCAH Brussels, 3-7 December 2007

1 Federal Agency for the Safety of the Food Chain NCD 2007

• No outbreaks in poultry • 8 outbreaks of PPMV-1 in racing pigeons

2 Federal Agency for the Safety of the Food Chain NCD 2007

3 Federal Agency for the Safety of the Food Chain NCD 2007

4 Federal Agency for the Safety of the Food Chain Measures in case of NCD in pigeons

• CD 92/66/EC is applied • All pigeons in the infected loft must be kept inside until 60 days after the last clinical symptoms • Zoning with transport restrictions and supplementary vaccination

5 Federal Agency for the Safety of the Food Chain NCD vaccination

• Mandatory vaccination of poultry • Mandatory vaccination of pigeons (racing, shows, other gatherings)

6 Federal Agency for the Safety of the Food Chain Website

www.favv.be www.afsca.be

Thank you for your attention

7 Federal Agency for the Safety of the Food Chain Overview of Newcastle disease in the Republic of Bulgaria

SCoFCAH 03- 04 December 2007 Outbreaks of ND for 2007

Since 01.January 2007 totally 13 outbreaks of ND have been reported in backyard poultry flocks in Republic of Bulgaria.

1. 08.01.2007 - village of Gigen, municipality of Guliantsi, administrative district of Pleven; 2. 06.02.2007- village of Kostelevo, municipality of Vratsa, administrative district of Vratsa; 3. 21.07.2007- village of Medeni polyani, municipality of Velingrad, administrative district of Pazardjik; 4. 30.08.2007 – primary outbreak in the village of Kravoder, municipality of Krivodol, administrative district Vratsa; 5. 12.09.2007– secondary outbreak in the village of Radovene, municipality of Roman, administrative district Vratsa; 6. 12.09.2007 - secondary outbreak in the village of Ruska bela, municipality of Mezdra, administrative district Vratsa; 7. 14.09.2007 - secondary outbreak in the village of Krapetc, municipality of Mezdra, administrative district Vratsa; 8. 17.09.2007 - secondary outbreak in the village of Smolyanovci, municipality of Montana, administrative district Montana; 9. 27.09.2007 - secondary outbreak in the village of Moravitsa, administrative district Vratsa; 10. 27.09.2007 - secondary outbreak in the village of Galatin, administrative district Vratsa; 11. 05.10.2007 - secondary outbreak in the village of Borovan, administrative district Vratsa; 12. 05.10.2007 - secondary outbreak in the village of Ohoden, administrative district Vratsa; 13. 23.09.2007 - primary outbreak in the village of Vidno, municipality Kavarna, administrative district of Dobrich; Outbreaks of ND in the Republic of Bulgaria for 2007 Presumed source of infection of ND in Vratsa and Montana region

{ the presumed source of infection is illegal trade of birds during the incubation period of the disease.

{ there is a national road connection (E 79) between the first outbreak in village of Kravoder and the secondary outbreaks in the Vratsa and Montana regions. { For control of the disease the National Veterinary Service of Republic of Bulgaria has taken all the measures provided for in Council Directive No. 92/66 of the European Union.

{ Protection zone of 3-km radius around the villages concerned and surveillance zone of 10-km radius around them have been immediately established. Number of affected birds

Region Number of dead birds Number of destroyed birds

Montana 132 690

Vratsa 62 195

Dobrich 12 34

Total 206 919

According to the Law of Veterinary Activity the owners of destroyed birds have been compensated by the national budget. Vaccination

{ The emergency vaccination was done of all birds located in the affected municipalities.

{ The vaccine was administered intranasally.

{ The vaccination was performed with the CEVAC NEW L – strain La Sota and re-vaccination 4 weeks after the initial vaccination with CEVAC NEW K - inactivated oil emulsion vaccine against Newcastle disease in birds; Vaccinated birds in the affected regions

Region Number of vaccinated Number of people, birds involved with the vaccination

Montana 346 259 88

Vratsa 431 921 179

Dobrich 81 657 13

Total 859 837 280 Necropsy { The diagnosis Newcastle disease was confirmed by the National Diagnostic and Research Veterinary Institute (NDRVI); { The analysis of the isolates by the Community Reference Laboratory- Weybridge shows that all isolates are 100% identical with each other and also with the isolates from 2006.

TheThe conclusionconclusion isis thatthat therethere isis aa circulationcirculation ofof thethe samesame virusvirus strainstrain withinwithin thethe country.country. Diagnostic (cloacal and tracheal swabs taken of 10 km. zones) by PCR

Region Number of Results tested samples Vratsa 489 Negative Montana 670 Negative Dobrich 70 Negative Total 1 229 Negative { With regard to the favourable epidemiological situation in Bulgaria with Orders of the Director General of NVS № 922, 923 and 924 of 26.10.2007 and № 970 of 09.11.2007, the restrictions in relation to ND outbreaks in the administrative districts of Vratsa, Montana and Dobrich have been lifted. As by now the epidemiological situation regarding the Newcastle disease in the Republic of Bulgaria is favorable and no further restriction zones are in place. Thank you for your attention! Newcastle disease in Estonia –2d outbreak

VFB – Ago Pärtel 03.12.2007 Newcastle disease in Estonia z 1962 z Findings from pigeons since november 2006 z August 2007 – OÜ Abja Muna 5100 laying hen z October 2007 – AS Tallegg 237 000 laying hen Newcastle disease - Tallegg

z Drop in egg production 10-30%, no mortality, no cöinical signs. z Samples taken 19.10.07 ( AI, ND, IBR, EDS) z 20.10.2007 - restrictions applied –no movements of poultry and eggs – ND seropositive result from VFL ( HAI, ELISA) z 24.10.2007 – VFL confirms ND virus isolation (PMV –1) from embryos z 25.10.2007 – samples sent to VLA Weybridge for strain confirmation and ICPI z 02.11.2007 - Weybridge interim report confirms virus with cleavage site motif KRQKRF ie virulent with 99,7% similarity with isolate from august 2007. z 05.11.2007 - Weybridge'i final report cofirms ICPI 1,625 Newcastle disease- Tallegg z 05.11.2007 VTA – ND officially confirmed z 05.11.2007 – contingency commission meeting z Official quarantine and restriction,surveillance zones with restrictions z VFB, Police, Rescue Board, municipal authorities, environmental authorities –Järelvalve tsoon 10 km – kõrgendatud kontroll linnukasvatajate üle –Ohustatud tsoon 3 km –farmidele kehtivad kitsendused Newcastle disease - Tallegg z 05 – 09.11.2007 237000 hen culled and buried z 10.11. 2007 1,7M eggs buried z 06.11.2007 decree by MoA for emergency vaccination in 3km zone z 06 – 08.11.2007 first vaccination - Tallegg 2 young layer farms with 144300 birds and 6 households with 100 birds. z 21 –23.11.2007 –secondary vaccination Newcastle disease - Tallegg z 06- 08.11.2007 inspection in restrictions zone 7 farms – 123959 birds surveillance zone 59 farms – 138255 birds No new suspects. 10 – 14.11.2007 cleaning and desinfection 22 – 23.11.2007 second desinfection 21 days standstill Newcastle disease - Estonia z Amendment to Animal Infectious Disease Act 01.01.2008 z Amendment to MoA regulation on ND surveillance. z Voluntary vaccination with VFB support 01.01 – 01.03.2008 z Obligatory vaccination for all holdings with more than 50 birds from 01.03.2008