Assessing sh diversity of Merbok Estuary and adjacent marine waters by DNA taxonomy: towards building comprehensive DNA barcode reference library Danial Hariz Zainal Abidin (
[email protected] ) Centre for Global Sustainability Studies (CGSS), Level 5, Hamzah Sendut Library, Universiti Sains Malaysia, 11800, Penang Siti Azizah Mohd. Nor Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030, Terengganu, Sébastien Lavoué School of Biological Sciences, Universiti Sains Malaysia, 11800, Penang Masazurah A. Rahim Fisheries Research Institute, Batu Maung, 11960, Penang Noorul Azliana Jamaludin Fisheries Research Institute, Kampung Acheh, 32000, Perak Noor Adelyna Mohammed Akib Centre for Global Sustainability Studies (CGSS), Level 5, Hamzah Sendut Library, Universiti Sains Malaysia, 11800, Penang Research Article Keywords: Mangrove Forests, Biodiversity, Morphological Examination, Molecular Approach, Fish Taxa Posted Date: March 6th, 2021 DOI: https://doi.org/10.21203/rs.3.rs-273253/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/28 Abstract The Merbok Estuary comprises one of the largest remaining mangrove forests in Peninsular Malaysia. Its value is signicant as it provides both direct and indirect services to local and global communities. It also offers a unique opportunity to study the structure and functioning of mangrove ecosystems. However, its biodiversity is still partially described, limiting its research value. Recent inventories based on morphological examination, documented 138 sh species residing, frequenting or subject to entering the Merbok Estuary. Using a molecular approach, we assessed the sh diversity of the Merbok Estuary and its adjacent waters in DNA barcoding 350 specimens assignable to 135 species initially identied based on morphology.