Environmental Microbiology: You Are What You

Total Page:16

File Type:pdf, Size:1020Kb

Environmental Microbiology: You Are What You RESEARCH HIGHLIGHTS ENVIRONMENTAL MICROBIOLOGY You are what you eat The bacteria in the human gut used this information to screen provide their host with energy by sequence databases for other putative degrading dietary polysaccharides. β-porphyranases. They found six contact with Writing in Nature, Hehemann et al. candidates, five of which were present now show that bacteria present in in marine bacteria. Interestingly, one environmental the gut of Japanese individuals have candidate (Bp1689) originated from microorganisms acquired a carbohydrate-degrading Bacteroides plebius, a bacterium iso- associated enzyme from marine bacteria that lated from the intestinal microbiota of with ingested allows them to degrade a polysaccha- Japanese individuals. To test whether ride found only in seaweed. porphyranases are common in food tailors While investigating the carbo- Japanese gut bacteria, Hehemann et the metabolic hydrate-active enzymes present in al. analysed the gut metagenome data capabilities of Zobellia galactanivorans, a member of from 13 Japanese individuals, iden- the marine Bacteroidetes, Hehemann tifying 7 different β-porphyranases our resident et al. identified five putative enzymes in 4 individuals. By contrast, the gut microbiota that distantly resembled glycoside gut metagenomes of 18 individuals hydrolases of the GH16 family but from North America contained no lacked the residues that provide β-porphyranases. Therefore, bacteria specificity for carbohydrates such that have acquired porphyran- as agarose or κ-carrageenan that are degrading enzymes, most likely found in marine algae. Two of these through horizontal gene transfer from enzymes were cloned and purified a marine bacterium, are common and then screened against a range in the gut microbiota of Japanese of natural polysaccharides extracted individuals, whose daily diet includes from various marine macrophytes. porphyran-containing seaweeds. The authors found that the enzymes These findings suggest that con- were active only against extracts from tact with environmental microorgan- red algae of the genus Porphyra, which isms associated with ingested food contain high levels of the polysaccha- tailors the metabolic capabilities of ride porphyran, leading the authors our resident gut microbiota, meaning to classify these enzymes as the first that there is some truth in the old β-porphyranases to be identified and adage, ‘you are what you eat’. to name them PorA and PorB. Andrew Jermy The authors then determined the crystal structures of PorA and ORIGINAL RESEARCH PAPER Hehemann, J. H. PorB, identified the key regions of et al. Transfer of carbohydrate-active enzymes the proteins that were important from marine bacteria to Japanese gut microbiota. Nature 464, 908–912 (2010) for binding to porphyran and GETTY NATURE REVIEWS | MICROBIOLOGY VOLUME 8 | JUNE 2010 © 2010 Macmillan Publishers Limited. All rights reserved.
Recommended publications
  • Gut Microbiota Beyond Bacteria—Mycobiome, Virome, Archaeome, and Eukaryotic Parasites in IBD
    International Journal of Molecular Sciences Review Gut Microbiota beyond Bacteria—Mycobiome, Virome, Archaeome, and Eukaryotic Parasites in IBD Mario Matijaši´c 1,* , Tomislav Meštrovi´c 2, Hana Cipˇci´cPaljetakˇ 1, Mihaela Peri´c 1, Anja Bareši´c 3 and Donatella Verbanac 4 1 Center for Translational and Clinical Research, University of Zagreb School of Medicine, 10000 Zagreb, Croatia; [email protected] (H.C.P.);ˇ [email protected] (M.P.) 2 University Centre Varaždin, University North, 42000 Varaždin, Croatia; [email protected] 3 Division of Electronics, Ruđer Boškovi´cInstitute, 10000 Zagreb, Croatia; [email protected] 4 Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia; [email protected] * Correspondence: [email protected]; Tel.: +385-01-4590-070 Received: 30 January 2020; Accepted: 7 April 2020; Published: 11 April 2020 Abstract: The human microbiota is a diverse microbial ecosystem associated with many beneficial physiological functions as well as numerous disease etiologies. Dominated by bacteria, the microbiota also includes commensal populations of fungi, viruses, archaea, and protists. Unlike bacterial microbiota, which was extensively studied in the past two decades, these non-bacterial microorganisms, their functional roles, and their interaction with one another or with host immune system have not been as widely explored. This review covers the recent findings on the non-bacterial communities of the human gastrointestinal microbiota and their involvement in health and disease, with particular focus on the pathophysiology of inflammatory bowel disease. Keywords: gut microbiota; inflammatory bowel disease (IBD); mycobiome; virome; archaeome; eukaryotic parasites 1. Introduction Trillions of microbes colonize the human body, forming the microbial community collectively referred to as the human microbiota.
    [Show full text]
  • The Gut Microbiota and Inflammation
    International Journal of Environmental Research and Public Health Review The Gut Microbiota and Inflammation: An Overview 1, 2 1, 1, , Zahraa Al Bander *, Marloes Dekker Nitert , Aya Mousa y and Negar Naderpoor * y 1 Monash Centre for Health Research and Implementation, School of Public Health and Preventive Medicine, Monash University, Melbourne 3168, Australia; [email protected] 2 School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane 4072, Australia; [email protected] * Correspondence: [email protected] (Z.A.B.); [email protected] (N.N.); Tel.: +61-38-572-2896 (N.N.) These authors contributed equally to this work. y Received: 10 September 2020; Accepted: 15 October 2020; Published: 19 October 2020 Abstract: The gut microbiota encompasses a diverse community of bacteria that carry out various functions influencing the overall health of the host. These comprise nutrient metabolism, immune system regulation and natural defence against infection. The presence of certain bacteria is associated with inflammatory molecules that may bring about inflammation in various body tissues. Inflammation underlies many chronic multisystem conditions including obesity, atherosclerosis, type 2 diabetes mellitus and inflammatory bowel disease. Inflammation may be triggered by structural components of the bacteria which can result in a cascade of inflammatory pathways involving interleukins and other cytokines. Similarly, by-products of metabolic processes in bacteria, including some short-chain fatty acids, can play a role in inhibiting inflammatory processes. In this review, we aimed to provide an overview of the relationship between the gut microbiota and inflammatory molecules and to highlight relevant knowledge gaps in this field.
    [Show full text]
  • Gut Microbiota: Its Role in Diabetes and Obesity
    ARTICLE Gut microbiota: Its role in diabetes and obesity Neil Munro Citation: Munro N (2016) Gut The gut microbiota is a community of microogranisms that live in the gut and intestinal microbiota: Its role in diabetes tract. The microbiota consists of bacteria, archaea and eukarya, as well as viruses, but is and obesity. Diabetes & Primary Care 18: 168–73 predominantly populated by anaerobic bacteria. Relationships between gut microbiota constituents and a wide range of human conditions such as enterocolitis, rheumatoid Article points arthritis, some cancers, type 1 and type 2 diabetes, and obesity have been postulated. 1. The role of the gut microbiota This article covers the essentials of the gut microbiota as well as the evidence for its role in certain disease progressions in diabetes and obesity. has been postulated, particularly its role in diabetes and obesity. he human gut microbiota is “an ecological Identifying microbiota constituents 2. There is much greater community of commensal, symbiotic and Until relatively recently, bacteria could only be genetic diversity between pathogenic micro-organisms that literally identified by direct microscopy and culture. people’s gut microbiota than T share our body space” (Lederberg and McCray, This proved particularly problematic with most between their genomes. 2001). It is made up of between 10 and 100 trillion anaerobic commensal gut flora (Ursell et al, 3. Improved understanding of the mechanisms by which the micro-organisms (with a mass weight of 1.5 kg) 2012). It has only been in recent years that human microbiota contributes and is found in the distal intestine (Allin et al, advances in gene sequencing and analytical to the development of 2015).
    [Show full text]
  • Association of Fungi and Archaea of the Gut Microbiota with Crohn's
    pathogens Article Association of Fungi and Archaea of the Gut Microbiota with Crohn’s Disease in Pediatric Patients—Pilot Study Agnieszka Krawczyk 1, Dominika Salamon 1,* , Kinga Kowalska-Duplaga 2 , Tomasz Bogiel 3,4 and Tomasz Gosiewski 1,* 1 Department of Molecular Medical Microbiology, Faculty of Medicine, Jagiellonian University Medical College, 31-121 Krakow, Poland; [email protected] 2 Department of Pediatrics, Gastroenterology and Nutrition, Faculty of Medicine, Jagiellonian University Medical College, 30-663 Krakow, Poland; [email protected] 3 Microbiology Department, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 85-094 Bydgoszcz, Poland; [email protected] 4 Clinical Microbiology Laboratory, University Hospital No. 1 in Bydgoszcz, 85-094 Bydgoszcz, Poland * Correspondence: [email protected] (D.S.); [email protected] (T.G.); Tel.: +48-(12)-633-25-67 (D.S.); +48-(12)-633-25-67 (T.G.) Abstract: The composition of bacteria is often altered in Crohn’s disease (CD), but its connection to the disease is not fully understood. Gut archaea and fungi have recently been suggested to play a role as well. In our study, the presence and number of selected species of fungi and archaea in pediatric patients with CD and healthy controls were evaluated. Stool samples were collected from children with active CD (n = 54), non-active CD (n = 37) and control subjects (n = 33). The prevalence and the number of selected microorganisms were assessed by real-time PCR. The prevalence of Candida Citation: Krawczyk, A.; Salamon, D.; tropicalis was significantly increased in active CD compared to non-active CD and the control group Kowalska-Duplaga, K.; Bogiel, T.; (p = 0.011 and p = 0.036, respectively).
    [Show full text]
  • Root Microbiota Assembly and Adaptive Differentiation Among European
    bioRxiv preprint doi: https://doi.org/10.1101/640623; this version posted May 17, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Root microbiota assembly and adaptive differentiation among European 2 Arabidopsis populations 3 4 Thorsten Thiergart1,7, Paloma Durán1,7, Thomas Ellis2, Ruben Garrido-Oter1,3, Eric Kemen4, Fabrice 5 Roux5, Carlos Alonso-Blanco6, Jon Ågren2,*, Paul Schulze-Lefert1,3,*, Stéphane Hacquard1,*. 6 7 1Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany 8 2Department of Ecology and Genetics, Evolutionary Biology Centre, Uppsala University, SE‐752 36 9 Uppsala, Sweden 10 3Cluster of Excellence on Plant Sciences (CEPLAS), Max Planck Institute for Plant Breeding Research, 11 50829 Cologne, Germany 12 4Department of Microbial Interactions, IMIT/ZMBP, University of Tübingen, 72076 Tübingen, 13 Germany 14 5LIPM, INRA, CNRS, Université de Toulouse, 31326 Castanet-Tolosan, France 15 6Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología (CNB), Consejo 16 Superior de Investigaciones Científicas (CSIC), 28049 Madrid, Spain 17 7These authors contributed equally: Thorsten Thiergart, Paloma Durán 18 *e-mail: [email protected], [email protected], [email protected] 19 20 Summary 21 Factors that drive continental-scale variation in root microbiota and plant adaptation are poorly 22 understood. We monitored root-associated microbial communities in Arabidopsis thaliana and co- 23 occurring grasses at 17 European sites across three years.
    [Show full text]
  • Microbiota and Obesogens: Environmental Regulators of Fat Storage
    Microbiota and obesogens: environmental regulators of fat storage John F. Rawls, Ph.D. Department of Molecular Genetics & Microbiology Center for Genomics of Microbial Systems Duke University School of Medicine Seminar outline 1. Introduction to the microbiota 2. Microbiota as environmental factors in obesity 3. Obesogens as environmental factors in obesity Seeing and understanding the unseen Antonie van Leewenhoek 1620’s: Described differences between microbes associated with teeth & feces during health & disease. Louis Pasteur 1850’s: Helps establish the “germ theory” of disease. 1885: Predicts that germ-free animals can not survive without “common microorganisms”. Culture-independent analysis of bacterial communities using 16S rRNA gene sequencing 16S rRNA 16S SSU rRNA 16S rRNA gene V1 V2 V3 V4 V5 V6 V7 V8 V9 Culture-independent analysis of bacterial communities using 16S rRNA gene sequencing plantbiology.siu.edu Phylogenetic analysis of 16S rRNA genes revealed 3 domains and a vast unappreciated diversity of microbial life You are here Carl Woese Glossary • Microbiota / microflora: the microorganisms of a particular site, habitat, or period of time. • Microbiome: the collective genomes of the microorganisms within a microbiota. • Germ-free / axenic: of a culture that is free from living organisms other than the host species. • Gnotobiotic: of an environment for rearing organisms in which all the microorganisms are either excluded or known. Current techniques for microbiome profiling Microbial community Morgan et al. (2012) Trends in
    [Show full text]
  • THE HUMAN MICROBIOTA: the ROLE of MICROBIAL COMMUNITIES in HEALTH and DISEASE La Microbiota Humana: Comunidades Microbianas En La Salud Y En La Enfermedad
    ACTA BIOLÓGICA COLOMBIANA http://www.revistas.unal.edu.co/index.php/actabiol SEDE BOGOTÁ FACULTAD DE CIENCIAS ARTÍCULODEPARTAMENTO DE DE REVISIÓN BIOLOGÍA INVITADO / INVITED REVIEW THE HUMAN MICROBIOTA: THE ROLE OF MICROBIAL COMMUNITIES IN HEALTH AND DISEASE La microbiota humana: comunidades microbianas en la salud y en la enfermedad Luz Elena BOTERO1,2, Luisa DELGADO-SERRANO3,4, Martha Lucía CEPEDA HERNÁNDEZ3, Patricia DEL PORTILLO OBANDO3, María Mercedes ZAMBRANO EDER3. 1 Facultad de Medicina, Universidad Pontificia Bolivariana. Calle 78B no. 72A-109, Bloque B, Piso 5. Medellín, Colombia. 2 Unidad de Bacteriología y Micobacterias, Corporación para Investigaciones Biológicas, Unidad Pontificia Bolivariana. Carrera 72A no. 78B-141. Medellín, Colombia. 3 Corporación Corpogen. Carrera 5 no. 66A-34. Bogotá D.C., Colombia. 4 Centro de Bioinformática y Biología Computacional- BIOS. Carrera 15B no. 161. Manizales, Colombia. For correspondence. [email protected] Received: 22nd March 2015, Returned for revision: 14th April 2015, Accepted: 10th July 2015. Associate Editor: Nubia Estela Matta Camacho. Citation / Citar este artículo como: Botero LE, Delgado-Serrano L, Cepeda ML, Del Portillo P, Zambrano MM. The human microbiota: the role of microbial communities in health and disease. Acta biol. Colomb. 2016;21(1):5-15. doi: http://dx.doi.org/10.15446/abc.v21n1.49761 ABSTRACT During the last decade, there has been increasing awareness of the massive number of microorganisms, collectively known as the human microbiota, that are associated with humans. This microbiota outnumbers the host cells by approximately a factor of ten and contains a large repertoire of microbial genome-encoded metabolic processes. The diverse human microbiota and its associated metabolic potential can provide the host with novel functions that can influence host health and disease status in ways that still need to be analyzed.
    [Show full text]
  • Aquatic Microbial Ecology 79:1
    Vol. 79: 1–12, 2017 AQUATIC MICROBIAL ECOLOGY Published online March 28 https://doi.org/10.3354/ame01811 Aquat Microb Ecol Contribution to AME Special 6 ‘SAME 14: progress and perspectives in aquatic microbial ecology’ OPENPEN ACCESSCCESS REVIEW Exploring the oceanic microeukaryotic interactome with metaomics approaches Anders K. Krabberød1, Marit F. M. Bjorbækmo1, Kamran Shalchian-Tabrizi1, Ramiro Logares2,1,* 1University of Oslo, Department of Biosciences, Section for Genetics and Evolutionary Biology (Evogene), Blindernv. 31, 0316 Oslo, Norway 2Institute of Marine Sciences (ICM), CSIC, Passeig Marítim de la Barceloneta, Barcelona, Spain ABSTRACT: Biological communities are systems composed of many interacting parts (species, populations or single cells) that in combination constitute the functional basis of the biosphere. Animal and plant ecologists have advanced substantially our understanding of ecological inter- actions. In contrast, our knowledge of ecological interaction in microbes is still rudimentary. This represents a major knowledge gap, as microbes are key players in almost all ecosystems, particu- larly in the oceans. Several studies still pool together widely different marine microbes into broad functional categories (e.g. grazers) and therefore overlook fine-grained species/population-spe- cific interactions. Increasing our understanding of ecological interactions is particularly needed for oceanic microeukaryotes, which include a large diversity of poorly understood symbiotic rela- tionships that range from mutualistic to parasitic. The reason for the current state of affairs is that determining ecological interactions between microbes has proven to be highly challenging. How- ever, recent technological developments in genomics and transcriptomics (metaomics for short), coupled with microfluidics and high-performance computing are making it increasingly feasible to determine ecological interactions at the microscale.
    [Show full text]
  • The Human Gut Microbiota: a Dynamic Interplay with the Host from Birth to Senescence Settled During Childhood
    Review nature publishing group The human gut microbiota: a dynamic interplay with the host from birth to senescence settled during childhood Lorenza Putignani1, Federica Del Chierico2, Andrea Petrucca2,3, Pamela Vernocchi2,4 and Bruno Dallapiccola5 The microbiota “organ” is the central bioreactor of the gastroin- producing immunological memory (2). Indeed, the intestinal testinal tract, populated by a total of 1014 bacteria and charac- epithelium at the interface between microbiota and lymphoid terized by a genomic content (microbiome), which represents tissue plays a crucial role in the mucosa immune response more than 100 times the human genome. The microbiota (2). The IS ability to coevolve with the microbiota during the plays an important role in child health by acting as a barrier perinatal life allows the host and the microbiota to coexist in a against pathogens and their invasion with a highly dynamic relationship of mutual benefit, which consists in dispensing, in modality, exerting metabolic multistep functions and stimu- a highly coordinated way, specific immune responses toward lating the development of the host immune system, through the biomass of foreign antigens, and in discriminating false well-­organized programming, which influences all of the alarms triggered by benign antigens (2). The failure to obtain growth and aging processes. The advent of “omics” technolo- or maintain this complex homeostasis has a negative impact gies (genomics, proteomics, metabolomics), characterized by on the intestinal and systemic health (2). Once the balance complex technological platforms and advanced analytical and fails, the “disturbance” causes the disease, triggering an abnor- computational procedures, has opened new avenues to the mal inflammatory response as it happens, for example, for the knowledge of the gut microbiota ecosystem, clarifying some inflammatory bowel diseases in newborns (2).
    [Show full text]
  • Optimize the Plant Microbiota to Increase Plant Growth and Health
    July 2019 POSITION PAPER Optimize the plant microbiota to increase plant growth and health Barret M. (INRA, IRHS), Dufour P. (RAGT), Durand-Tardif M. (GIS BV), Mariadassou M. (INRA, MaIAGE), Mougel C. (INRA, IGEPP), Perez P. (Limagrain), Roumagnac P. (Cirad, BGPI), Sanguin H. (Cirad, BGPI), Steinberg C. (INRA, Agroécologie), Szambien M. (GIS BV) The “Groupement d’Intérêt Scientifique Biotechnologies Vertes” (GIS BV) organized on November 13th, 2018 in Paris, a scientific workshop on “Metagenomics for agro-ecosystems management and plant breeding”. Thirty-four scientists, including eight from the private sector attended the workshop. General discussion was organized around the presentations related to plant, seeds and soil microbiota, and data treatment to reconstruct interaction networks. This article gathers the current French research strengths, relative to the international context and highlights the research priorities between the public and the private sectors, using plant genetics and plant-microbiota interactions for the benefit of future agricultures. plant productivity. Hence, over recent years a Socio-economic context, scientific number of research groups explored the impact of challenges and opportunities environmental factors and host genetic variation on the composition and dynamics of plant microbiota [12–19]. Overall, these studies Plants live in association with a wide diverse and acknowledged an important influence of the complex assembly of viruses and microorganisms environment on plant microbiota composition and including
    [Show full text]
  • Corals and Sponges Under the Light of the Holobiont Concept: How Microbiomes Underpin Our Understanding of Marine Ecosystems
    fmars-08-698853 August 11, 2021 Time: 11:16 # 1 REVIEW published: 16 August 2021 doi: 10.3389/fmars.2021.698853 Corals and Sponges Under the Light of the Holobiont Concept: How Microbiomes Underpin Our Understanding of Marine Ecosystems Chloé Stévenne*†, Maud Micha*†, Jean-Christophe Plumier and Stéphane Roberty InBioS – Animal Physiology and Ecophysiology, Department of Biology, Ecology & Evolution, University of Liège, Liège, Belgium In the past 20 years, a new concept has slowly emerged and expanded to various domains of marine biology research: the holobiont. A holobiont describes the consortium formed by a eukaryotic host and its associated microorganisms including Edited by: bacteria, archaea, protists, microalgae, fungi, and viruses. From coral reefs to the Viola Liebich, deep-sea, symbiotic relationships and host–microbiome interactions are omnipresent Bremen Society for Natural Sciences, and central to the health of marine ecosystems. Studying marine organisms under Germany the light of the holobiont is a new paradigm that impacts many aspects of marine Reviewed by: Carlotta Nonnis Marzano, sciences. This approach is an innovative way of understanding the complex functioning University of Bari Aldo Moro, Italy of marine organisms, their evolution, their ecological roles within their ecosystems, and Maria Pia Miglietta, Texas A&M University at Galveston, their adaptation to face environmental changes. This review offers a broad insight into United States key concepts of holobiont studies and into the current knowledge of marine model *Correspondence: holobionts. Firstly, the history of the holobiont concept and the expansion of its use Chloé Stévenne from evolutionary sciences to other fields of marine biology will be discussed.
    [Show full text]
  • Succession and Cazyme Expression of Marine Bacterial
    bioRxiv preprint doi: https://doi.org/10.1101/2020.12.08.416354; this version posted December 8, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Sweet and magnetic: Succession and CAZyme expression of marine bacterial communities encountering a mix of alginate and pectin particles Carina Bunse1,2,*, Hanna Koch3,4,*, Sven Breider3, Meinhard Simon1,3 and Matthias Wietz2,3# 1Helmholtz Institute for Functional Marine Biodiversity at the University of Oldenburg, Germany 2Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Germany 3Institute for Chemistry and Biology of the Marine Environment, University of Oldenburg, Germany 4Department of Microbiology, Radboud University Nijmegen, The Netherlands *These authors contributed equally to this work. #Corresponding author HGF-MPG Joint Research Group for Deep-Sea Ecology and Technology Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research Am Handelshafen 12 27570 Bremerhaven Germany Email: [email protected] Telephone +49-471-48311454 bioRxiv preprint doi: https://doi.org/10.1101/2020.12.08.416354; this version posted December 8, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. ABSTRACT Polysaccharide particles are an important nutrient source and microhabitat for marine bacteria. However, substrate-specific bacterial dynamics in a mixture of particle types with different polysaccharide composition, as likely occurring in natural habitats, are undescribed.
    [Show full text]