DOCSLIB.ORG

(12) Patent Application Publication (10) Pub. No.: US 2012/0009150 A1 WEBER Et Al

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text

Load more

US 2012O009 150A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2012/0009150 A1 WEBER et al. (43) Pub. Date: Jan. 12, 2012 (54) DIARYLUREAS FORTREATINGVIRUS Publication Classification INFECTIONS (51) Int. Cl. (76) Inventors: Olaf WEBER, Wulfrath (DE); st 2. CR Bernd Riedl, Wuppertal (DE) ( .01) A63/675 (2006.01) (21) Appl. No.: 13/236,865 A6II 3/522 (2006.01) A6IP 29/00 (2006.01) (22) Filed: Sep. 20, 2011 A6II 3/662 (2006.01) A638/14 (2006.01) Related U.S. Application Data A63L/7056 (2006.01) A6IP3L/2 (2006.01) (63) Continuation of application No. 12/097.350. filed on A6II 3/44 (2006.01) Nov. 3, 2008, filed as application No. PCTAEPO6/ A6II 3/52 (2006.01) 11693 on Dec. 6, 2006. O O (52) U.S. Cl. .......... 424/85.6; 514/350; 514/171; 514/81; (30) Foreign Application Priority Data 514/263.38: 514/263.4: 514/120: 514/4.3: Dec. 15, 2005 (EP) .................................. 05O274513 424/85.7; 514/43 Dec. 15, 2005 (EP). ... O5O27452.1 Dec. 15, 2005 (EP). ... O5O27456.2 Dec. 15, 2005 (EP). ... O5O27458.8 The present invention relates to pharmaceutical compositions Dec. 15, 2005 (EP) O5O27.460.4 for treating virus infections and/or diseases caused by virus Dec. 15, 2005 (EP) O5O27462.O infections comprising at least a diary1 urea compound option Dec. 15, 2005 (EP). ... O5O27465.3 ally combined with at least one additional therapeutic agent. Dec. 15, 2005 (EP). ... O5O274.67.9 Useful combinations include e.g. BAY 43-9006 as a diaryl Dec. 15, 2005 (EP) .................................. 05O27471.1 urea compound. US 2012/0009 150 A1 Jan. 12, 2012 DARYLUREAS FORTREATINGVIRUS oviruses such as human Varizella Zoster virus), Betaherpes INFECTIONS viridae (e.g. cytomegalovirus and roseolovirus) and Gamma herpesviridae (e.g. Epstein-Barr virus). Such virus infections can cause e.g. infections of the lymphatic system of the outer 0001. The present invention relates to pharmaceutical genitalia, the lips, the briars (herpesencephalitis) or the compositions for treating virus infections and/or diseases peripheral nerves. caused thereby comprising at least a diaryl urea compound 0009. A number of herpeviruses use the cellular signal optionally combined with at least one additional therapeutic pathways of MAPK/ERK and p38 MAPK, e.g. infection with agent. Useful combinations include e.g. BAY 43-9006 as a herpes simplex virus induce the activation of the p38 MAPK diaryl urea compound. and SAPK/JNK signal pathway (G. Zachos et al., J. Biol. 0002 BAY 43-9006 refers to 4-4-3-(4-chloro-3-trifluo Chem. 1999, 274, 5097). Inhibitors of the MAPK/ERK or the romethylphenyl)-ureidol-phenoxy-pyridine-2-carboxylic p38 MAPK pathway inhibit the activation of early promoters acid methyl amide and is species of diaryl urea compounds of the human cytomegalovirus (J. Chen et al. J. Virol., 2002, which are potent anti-cancer and anti-angiogenic agents that 76 (10), 4873). possess various activities, including inhibitory activity on the 0010. The viruses of the Papovaviridae family comprise VEGFR, PDGFR, rat p38, and/or fit-3 kinase signaling mol the genus papillomaviruses and include a “high risk group of ecules. See, e.g., WO 2004/113274 and WO 2005/000284. viruses (e.g. species HPV 16, 18) and a “low risk” group (e.g. 0003 SARS (severe acute respiratory syndrome) is a dis HPV 6, 11). Human papillomaviruses induce neoplasm of the ease caused by an infection with SARS coronavirus (SARS dermis and can cause the formation of papillomas. Virus CoV) which gets public importance in the last years. For infections of the “low risk” group, however, are associated infected patients the therapeutic standard of today is, how with malignant tumour diseases (e.g. Zervix cancer). Types of ever, low. the “low risk group cause e.g. anogenital warts. An activa 0004. A typical coronavirus is represented by e.g. the tion of the MAPK signal pathway is detected in human pap mouse hepatitis virus (MHV) which induces the p38 kinase illomas infected with papillomaviruses (D. Johnston et al., which is part of the MAPK pathway in infected cells (S. Cancer Res., 1999, 59 (4), 968). Banerjee et al. J. Viral. 2002, 76,5937-5948). Furthermore 0011 Pox were one of the most dreaded diseases in history recent results show that also SARS-CoV induces the signal and deemed to be exterminated in 1977 after introduction of pathway of p38 MAPK in permissive cells (Mizutani et al. immunisation. Today poxviruses Such as the molluscum con Biochem. Biophys. Res. Commun. 2004, 319, 1228-1234). tagiosum virus and poxviruses pathogenic for animals play a 0005. A known standard therapy of HIV (human immu role. The viruses of the Poxyiridae family include the sub nodeficiency virus) infections is HAART (highly active anti family Chordopoxyiridae and comprise avipoxvirus, capri retroviral therapy) wherein a combination of several antiret poxvirus, lepripoxvirus, Suipoxvirus, parapoxvirus, mollus roviral drugs (protease inhibitors and antiretroviral drugs) are cipoxvirus and orthopoxvirus. Such virus infections can administered to infected patients (e.g. a combination of indi cause e.g. Smallpox. Cellular targets are known for the navir, zidovudine and lamivudin). The drugs inhibit the abil therapy of poxvirus infections (H. Yang et al., J. Clin. Invest, ity of the virus to multiply in the body and slow the develop 2005, 115 (2),379). ment of AIDS (acquired immunodeficiency syndrome). 0012. The genus flavivirus and pestivirus especially the 0006 Furthermore it is known that the p38 kinase inhibi yellow fever virus, denguevirus 1 to 4, west nile fever virus, tor RWJ 67657 suppresses the replication of HIV and the spring-Summer encephalitis virus, Omsk-hemorrhagic fever cellular pathogenesis of the infection (K. Muthumani et al. virus, bovine virus-diarrhea-Virus and swine fever virus, AIDS, 2004, 18, 739-748). belong to the Flaviviridae family. Such virus infections can 0007 Hepatitis viruses such as HBV and HCV modulate cause e.g. encephalitis and encephalomyelitis. the MAPK signal pathway in infected cells (M. Panteva et al. Virus Research 2003, 92, 131). A permanent activation of the 0013 Activation of the p38 MAPK signal pathway plays RAF/MEK/ERK signal pathway is detected in cells express an important role for the interaction of Flaviviridae viruses ing HCV Core Protein (S. Giambartolomei et al. Oncogene, and the host cells (C. Chen et al., J. Gen. Viral. 2002, 83, 2001, 20, 2607) and an increased level of N-Ras is important 1897). for the maintenance of the replication of HCV (P. Mannova, 0014. The genus enterovirus, cardiovirus, rhinovirus, aph L. Beretta, J. Virol. 2005, 79 (14), 8742) wherein Ras is tovirus and hepatovirus especially the polio-viruses, coxsack affected by Raf. It is also known that the integrity of the ieviruses, coxsackieviruses, human echoviruses, human RAF/MEK signal cascade is a precondition for the replication enteroviruses, human rhinoviruses and hanks viruses, belong of HBV (L. Stockl, Oncogene, 2003, 22 (17), 260). Influenza to the Picornaviridae family. Such virus infections can cause viruses such as type A, B or C belong to group of OrthomyX e.g. in humans aseptic meningitis, poliomyelitis, herpangina, oviruses and cause every year flu epidemics effecting up to pleurodynia (Bornholm disease), myositis, rhabdomyolysis, 10,000 cases of death per year in Germany. Relevant cellular diabetes type I, summer fever and myocarditis. Furthermore targets for a therapy are known (S. Ludwig et al., Trends Mol. in animals rhinoviruses, and the foot and mouth disease Med., 2003, 46). The p38 MAPK signal pathway is induced in viruses can be caused by Such infections. mouse cells infected with influenza A virus (I. Mori et al., J. (0015. It is shown that inhibition of p38 MAPK can inhibit Gen. Virol. 2003, 84, 2401). Furthermore inhibition of MEK the replication of Picornaviridae viruses (K. Hirasawa et al., J. inhibit the proliferation of influenza V virus in cell cultures Virol. 2003, 77 (10), 5649-5656). (S. Ludwig et al. FEBS Letters, 2004, 561,37). 0016. The present invention provides pharmaceutical 0008. The viruses of the Herpesviridae family comprise compositions for treating virus infections and/or diseases viruses of the Sub-families Alphaherpesviridae (e.g. simplcX caused thereby comprising at least one compound of formula viruses such as human herpes simplex viruses and Varicell I and optionally at least one further therapeutic agent. US 2012/0009 150 A1 Jan. 12, 2012 0017. The present invention provides a therapeutic diseases caused by Such infections comprising at least one method which treat virus infections according to the present compound of formula I and optionally at least one further invention and/or diseases caused by Such infections of therapeutic agent. infected patients more effectively compared to current thera 0027. The present invention provides a therapeutic pies and therefore is Superior to current therapies. The present method which treat Elerpesviridae viruses infections and/or invention can be used e.g. by administering a diary1 urea diseases caused by Such infections of infected patients more compound of formula I and optionally a further therapeutic effectively compared to current therapies and therefore is agent, pharmaceutically-acceptable salts thereof, and deriva Superior to current therapies. The present invention can be tives thereof, etc. used e.g. by administering a diary1 urea compound of formula 0018. The present invention provides pharmaceutical I and optionally a further therapeutic agent, pharmaceuti compositions
Recommended publications
  • Vaccinia Belongs to a Family of Viruses That Is Closely Related to the Smallpox Virus

    Vaccinia Belongs to a Family of Viruses That Is Closely Related to the Smallpox Virus

    VACCINIA INFECTION What is it? Vaccinia belongs to a family of viruses that is closely related to the smallpox virus. Because of the similarities between the smallpox and vaccinia viruses, the vaccinia virus is used in the smallpox vaccine. When this virus is used as a vaccine, it allows our immune systems to develop immunity against smallpox. The smallpox vaccine does not actually contain smallpox virus and cannot cause smallpox. Vaccination usually prevents smallpox infection for at least ten years. The vaccinia vaccine against smallpox was used to successfully eradicate smallpox from the human population. More recently, this virus has also become of interest due to concerns about smallpox being used as an agent of bioterrorism. How is the virus spread? Vaccinia can be spread by touching the vaccination site before it has fully healed or by touching clothing or bandages that have been contaminated with the live virus during vaccination. In this manner, vaccinia can spread to other parts of the body and to other individuals. It cannot be spread through the air. What are the symptoms of vaccinia? Vaccinia virus symptoms are similar to smallpox, but milder. Vaccinia may cause rash, fever, headache and body aches. In certain individuals, such as those with weak immune systems, the symptoms can be more severe. What are the potential side effects of the vaccinia vaccine for smallpox? Normal reactions are mild and go away without any treatment.These include: Soreness and redness in the arm where the vaccine was given Slightly swollen, sore glands in the armpits Low grade fever One in approximately three people will feel badly enough to miss school, work or recreational activities Trouble sleeping Serious reactions are not very common but can occur in about 1,000 in every 1 million people who are vaccinated for the first time.
  • Cyprinus Carpio

    Cyprinus Carpio

    Académie Universitaire Wallonie - Europe Université de Liège Faculté de Médecine Vétérinaire Département des Maladies Infectieuses et Parasitaires Service d’Immunologie et de Vaccinologie Etude des portes d’entrée de l’Herpèsvirus cyprin 3 chez Cyprinus carpio Study of the portals of entry of Cyprinid herpesvirus 3 in Cyprinus carpio Guillaume FOURNIER Thèse présentée en vue de l’obtention du grade de Docteur en Sciences Vétérinaires Année académique 2011-2012 Académie Universitaire Wallonie - Europe Université de Liège Faculté de Médecine Vétérinaire Département des Maladies Infectieuses et Parasitaires Service d’Immunologie et de Vaccinologie Etude des portes d’entrée de l’Herpèsvirus cyprin 3 chez Cyprinus carpio Study of the portals of entry of Cyprinid herpesvirus 3 in Cyprinus carpio Promoteur : Prof. Alain Vanderplasschen Guillaume FOURNIER Thèse présentée en vue de l’obtention du grade de Docteur en Sciences Vétérinaires Année académique 2011-2012 « La science progresse en indiquant l'immensité de l'ignoré. » Louis Pauwels Remerciements Liège, le 15 février 2012 L’accomplissement d’une thèse est un long et palpitant voyage en océan où se mélangent la curiosité, le doute, la persévérance, et la confiance… en soi bien sûr, mais surtout envers toutes les personnes qui, par leurs conseils, leur aide, leur soutien m’ont permis de mener cette thèse à bien. Je tiens ici à remercier mes collègues, amis et famille qui ont été tantôt les phares, tantôt les boussoles, toujours les fidèles compagnons de cette aventure. Je commencerais par adresser mes plus sincères remerciements à mon promoteur, le Professeur Alain Vanderplasschen, qui m’avait déjà remarqué en amphithéâtre pour ma curiosité, à moins que ce ne soit pour mon irrésistible coiffure..
  • HS and Product Codes

    HS and Product Codes

    NATIONAL PARKS BOARD / ANIMAL AND VETERINARY SERVICE (NPARKS/AVS) INDUSTRY AND BIOSECURITY MANAGEMENT GROUP LICENSING AND COMPLIANCE DEPARTMENT LICENSING AND PERMITS SECTION 52 JURONG GATEWAY ROAD, JEM OFFICE TOWER, #09-01 SINGAPORE 608550 HS CODE PRODUCT CODE PRODUCT DESCRIPTION QTY UNIT A) LIVE ANIMALS & BIRDS AND VETERINARY BIOLOGICS AMPHIBIANS (LIVE FROGS) 01069000 VAP0FF LIVE FROGS FOR FISH FEEDING NMB 01069000 VAP0ZZ LIVE FROGS (NON-CITES) NMB 01069000 VAP1ZZ LIVE FROGS (CITES LISTED) NMB BIRDS (CAPTIVE BIRDS) 01063100 VBD1BP BIRDS OF PREY 01063200 VBD1ZZ LIVE BIRDS (CITES LISTED) NMB 01063900 VBD0ZZ LIVE BIRDS (NON-CITES) NMB BREEDING ANIMALS (FOR BREEDING PURPOSE ONLY) 01011000 VBA0HO LIVE HORSES FOR BREEDING NMB LABORATORY ANIMALS (MICE/RATS/GUINEA PIGS/HAMSTERS/RABBITS) 01061900 VLA0GP LIVE GUINEA PIGS NMB 01061900 VLA0HA LIVE HAMSTERS NMB 01061900 VLA0MC LIVE MICE NMB 01061900 VLA0RA LIVE RABBITS NMB 01061900 VLA0RT LIVE RATS NMB 01061900 VLA0ZZ OTHER LABORATORY ANIMALS NMB PET ANIMALS (DOGS/CATS/HORSES/RABBITS/GUINEA PIGS) 01019030 VPA0HO LIVE HORSE FOR RACING NMB 01019030 VPA0PO LIVE PONIES NMB 01061900 VPA0CA LIVE CATS (COMMERCIAL) NMB 01061900 VPA0CP LIVE CATS (PERSONAL) NMB 01061900 VPA0CL LIVE CHINCHILLAS NMB 01061900 VPA0DG LIVE DOGS (COMMERCIAL) NMB 01061900 VPA0DP LIVE DOGS (PERSONAL) NMB 01061900 VPA0GP LIVE GUINEA PIGS NMB 01061900 VPA0HA LIVE HAMSTERS NMB 01061900 VPA0RA LIVE RABBITS NMB 01061900 VPA0ZZ OTHER PET ANIMALS NMB WILD ANIMALS (ZOO ANIMALS) 01061900 VWA0ZZ ZOO ANIMALS (NON-CITES) NMB 01061900 VWA1ZZ ZOO ANIMALS (CITES LISTED) NMB 01061100 VWA1PM PRIMATES NMB 01061200 VWA1DG MANATEES/DUGONGS NMB 01061200 VWA1WD WHALES/DOLPHINS NMB VETERINARY VACCINES 30023000 VVC0ZZ VETERINARY VACCINE - VETERINARY BIOLOGICS 30029000 VVP0B0COWRUMB COWDRIA RUMINANTIUM - 30029000 VVP0B2AERHYDB AEROMONAS HYDROPHILA - 30029000 VVP0B2ANAMARB ANAPLASMA MARGINALE - 30029000 VVP0B2BORBURB BORRELIA BURGDORFERI - 30029000 VVP0B2CAMFETV CAMPYLOBACTER FETUS SUBSP.
  • An Assay Redesign and Evaluation

    An Assay Redesign and Evaluation

    Deficiencies in the current assays for the detection and identification of DNA viruses of carp: an assay redesign and evaluation. David Stone1, Peng Jia2 and Hong Liu2 1Cefas Weymouth Laboratory, UK 2Shenzhen Exit & Entry Inspection and Quarantine Bureau, People's Republic of China. World Class Science for the Marine and Freshwater Environment Overview • BREXIT • Cyprinivirus-specific primers • Failures in CyHV-3 detection using the Gilad qPCR assay • Design and initial evaluation of a CyHV-3 pol qPCR assay • CEV • Current PCR based assays • Failures in the Cefas conventional PCR assay • Design and initial evaluation of a modified nested PCR assay • Work to be done KHV (Cyprinid herpesvirus 3) • Large DNA virus (295 kbp genome) – of the Alloherpesviridae family in the order Herpesvirales • CyHV-3 (Koi herpesvirus - KHV) is the type species of the Cyprinivirus genus -also contains Cyprinid herpesviruses 1 & 2 and Anguillid herpesvirus • Disease affects Common carp (Cyprinus carpio), including ornamental koi carp and varieties and hybrids such as mirror and ghost carp. Goldfish (Carassius auratus) x common carp hybrids also have low susceptibility to CyHV-3 infection Cyprinivirus- specific DNA polymerase primers Nested conventional PCR assay based on CyHV 1-3 DNA polymerase sequences • Analytical sensitivity of 1-10 copies/reaction (~DNA from 0.25mg tissue) • Assay accredited to ISO 17025 Initially run in parallel to the TK primers recommended by the OIE. In the UK the assay was adopted as the primary assay for confirmation of disease outbreaks
  • Cidofovir Activity Against Poxvirus Infections

    Cidofovir Activity Against Poxvirus Infections

    Viruses 2010 , 2, 2803-2830; doi:10.3390/v2122803 OPEN ACCESS viruses ISSN 1999-4915 www.mdpi.com/journal/viruses Review Cidofovir Activity against Poxvirus Infections Graciela Andrei * and Robert Snoeck Laboratory of Virology and Chemotherapy, Rega Institute for Medical Research, KULeuven, Minderboredersstraat 10, B-3000 Leuven, Belgium; E-Mail: [email protected] * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +32-16-337372; Fax: +32-16-337340. Received: 10 November 2010; in revised form: 9 December 2010 / Accepted: 10 December 2010 / Published: 22 December 2010 Abstract: Cidofovir [(S)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine, HPMPC] is an acyclic nucleoside analog approved since 1996 for clinical use in the treatment of cytomegalovirus (CMV) retinitis in AIDS patients. Cidofovir (CDV) has broad-spectrum activity against DNA viruses, including herpes-, adeno-, polyoma-, papilloma- and poxviruses. Among poxviruses, cidofovir has shown in vitro activity against orthopox [vaccinia, variola (smallpox), cowpox, monkeypox, camelpox, ectromelia], molluscipox [molluscum contagiosum] and parapox [orf] viruses. The anti-poxvirus activity of cidofovir in vivo has been shown in different models of infection when the compound was administered either intraperitoneal, intranasal (aerosolized) or topically. In humans, cidofovir has been successfully used for the treatment of recalcitrant molluscum contagiosum virus and orf virus in immunocompromised patients. CDV remains a reference compound against poxviruses and holds potential for the therapy and short-term prophylaxis of not only orthopox- but also parapox- and molluscipoxvirus infections. Keywords: cidofovir; poxviruses; acyclic nucleoside analog 1. Introduction The antiviral activity of (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine (HPMPC, cidofovir, CDV) (Figure 1) against human cytomegalovirus (HCMV) and other DNA viruses was first Viruses 2010 , 2 2804 reported in 1986 [1].
  • Molecular Identification and Genetic Characterization of Cetacean Herpesviruses and Porpoise Morbillivirus

    Molecular Identification and Genetic Characterization of Cetacean Herpesviruses and Porpoise Morbillivirus

    MOLECULAR IDENTIFICATION AND GENETIC CHARACTERIZATION OF CETACEAN HERPESVIRUSES AND PORPOISE MORBILLIVIRUS By KARA ANN SMOLAREK BENSON A THESIS PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE UNIVERSITY OF FLORIDA 2005 Copyright 2005 by Kara Ann Smolarek Benson I dedicate this to my best friend and husband, Brock, who has always believed in me. ACKNOWLEDGMENTS First and foremost I thank my mentor, Dr. Carlos Romero, who once told me that love is fleeting but herpes is forever. He welcomed me into his lab with very little experience and I have learned so much from him over the past few years. Without his excellent guidance, this project would not have been possible. I thank my parents, Dave and Judy Smolarek, for their continual love and support. They taught me the importance of hard work and a great education, and always believed that I would be successful in life. I would like to thank Dr. Tom Barrett for the wonderful opportunity to study porpoise morbillivirus in his laboratory at the Institute for Animal Health in England, and Dr. Romero for making the trip possible. I especially thank Dr. Ashley Banyard for helping me accomplish all the objectives of the project, and all the wonderful people at the IAH for making a Yankee feel right at home in the UK. I thank Alexa Bracht and Rebecca Woodruff who have been with me in Dr. Romero’s lab since the beginning. Their continuous friendship and encouragement have kept me sane even in the most hectic of times.
  • Evaluation of Cyprinid Herpesvirus 2 Latency and Reactivation in Carassius Gibel

    Evaluation of Cyprinid Herpesvirus 2 Latency and Reactivation in Carassius Gibel

    microorganisms Article Evaluation of Cyprinid Herpesvirus 2 Latency and Reactivation in Carassius gibel 1, 1, 1 1 2 1,3 Wenjun Chai y, Lin Qi y, Yujun Zhang , Mingming Hong , Ling Jin , Lijuan Li and Junfa Yuan 1,3,* 1 Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; [email protected] (W.C.); [email protected] (L.Q.); [email protected] (Y.Z.); [email protected] (M.H.); [email protected] (L.L.) 2 Department of Biomedical Science, Carlson College of Veterinary Medicine, Oregon State University, Corvallis, OR 97330, USA; [email protected] 3 Hubei Engineering Research Center for Aquatic Animal Diseases Control and Prevention, Wuhan 430070, China * Correspondence: [email protected] These authors contribute equal. y Received: 20 January 2020; Accepted: 19 March 2020; Published: 21 March 2020 Abstract: Cyprinid herpesvirus 2 (CyHV-2, species Cyprinid herpesvirus 2) causes severe mortality in ornamental goldfish, crucian carp (Carassius auratus), and gibel carp (Carassius gibelio). It has been shown that the genomic DNA of CyHV-2 could be detected in subclinical fish, which implied that CyHV-2 could establish persistent infection. In this study, the latency of CyHV-2 was investigated in the survival fish after primary infection. CyHV-2 genomic DNA was detected in multiple tissues of acute infection samples; however, detection of CyHV-2 DNA was significantly reduced in fish recovered from the primary infection on day 300 postinfection. No active viral gene transcription, such as DNA polymerase and ORF99, was detected in recovered fish.
  • "Fischgesundheit Und Fischerei Im Wandel Der Zeit"

    "Fischgesundheit Und Fischerei Im Wandel Der Zeit"

    Fischgesundheit und Fischerei im Wandel der Zeit Tagungsband XV. Gemeinschaftstagung der Deutschen, Österreichischen und Schweizer Sektionen der European Association of Fish Pathologists (EAFP) Starnberg, 8. – 10. Oktober 2014 Die Tagung wurde in wesentlichen Teilen finanziert vom Bayerischen Staatsministerium für Ernährung, Landwirtschaft und Forsten (StMELF) aus der Fischereiabgabe Bayerns Weitere Unterstützung erfolgte durch: ― Niedersächsisches Landesamt für Verbraucherschutz und Lebensmittelsicherheit ― Bayerische Landesanstalt für Landwirtschaft, Institut für Fischerei ― MSD Tiergesundheit, Intervet Deutschland GmbH ― Zentralverband Zoologischer Fachbetriebe (ZZF) und Wirtschaftsgemeinschaft Zoolo- gischer Fachbetriebe GmbH (WZF) ― Familie Gerda und Hartmut Stachowitz ― Oswald Fürneisen ― Tetra GmbH, Melle ― BioMar Group Für die Erstellung des Tagungsbandes wurden die von den Autoren eingesandten Manu- skripte bzw. Zusammenfassungen verwendet. Für die Inhalte und Abbildungen sind die Autoren verantwortlich. Einige Beiträge wurden oder werden an anderer Stelle veröffent- licht. Im vorliegenden Tagungsband sind Zusammenfassungen dieser Beiträge veröffent- licht. Zitiervorschlag KLEINGELD, D. W., und WEDEKIND, H. (Hrsg.) (2015): Fischgesundheit und Fische- rei im Wandel der Zeit. XV. Gemeinschaftstagung der Deutschen, Österreichischen und Schweizer Sektion der European Association of Fish Pathologists (EAFP), 8. – 10. Okto- ber 2014 an der LfL in Starnberg. Impressum Herausgeber: Bayerische Landesanstalt für Landwirtschaft, Vöttinger
  • Fish Herpesvirus Diseases

    Fish Herpesvirus Diseases

    ACTA VET. BRNO 2012, 81: 383–389; doi:10.2754/avb201281040383 Fish herpesvirus diseases: a short review of current knowledge Agnieszka Lepa, Andrzej Krzysztof Siwicki Inland Fisheries Institute, Department of Fish Pathology and Immunology, Olsztyn, Poland Received March 19, 2012 Accepted July 16, 2012 Abstract Fish herpesviruses can cause significant economic losses in aquaculture, and some of these viruses are oncogenic. The virion morphology and genome organization of fish herpesviruses are generally similar to those of higher vertebrates, but the phylogenetic connections between herpesvirus families are tenuous. In accordance with new taxonomy, fish herpesviruses belong to the family Alloherpesviridae in the order Herpesvirales. Fish herpesviruses can induce diseases ranging from mild, inapparent infections to serious ones that cause mass mortality. The aim of this work was to summarize the present knowledge about fish herpesvirus diseases. Alloherpesviridae, CyHV-3, CyHV-2, CyHV-1, IcHV-1, AngHV-1 Herpesviruses comprise a numerous group of large DNA viruses with common virion structure and biological properties (McGeoch et al. 2008; Mattenleiter et al. 2008). They are host-specific pathogens. Apart from three herpesviruses found recently in invertebrate species, all known herpesviruses infect vertebrates, from fish to mammals (Davison et al. 2005a; Savin et al. 2010). According to a new classification accepted by the International Committee on Taxonomy of Viruses (http:/ictvonline.org), all herpesviruses have been incorporated into a new order named Herpesvirales, which has been split into three families. The revised family Herpesviridae contains mammalian, avian, and reptilian viruses; the newly-created family Alloherpesviridae contains herpesviruses of fish and amphibians, and the new family Malacoherpesviridae comprises single invertebrate herpesvirus (Ostreid herpesvirus).
  • Koi Herpesvirus Disease (KHVD)1 Kathleen H

    Koi Herpesvirus Disease (KHVD)1 Kathleen H

    VM-149 Koi Herpesvirus Disease (KHVD)1 Kathleen H. Hartman, Roy P.E. Yanong, Deborah B. Pouder, B. Denise Petty, Ruth Francis-Floyd, Allen C. Riggs, and Thomas B. Waltzek2 Introduction Koi herpesvirus (KHV) is a highly contagious virus that causes significant morbidity and mortality in common carp (Cyprinus carpio) varieties (Hedrick et al. 2000, Haenen et al. 2004). Common carp is raised as a foodfish in many countries and has also been selectively bred for the ornamental fish industry where it is known as koi. The first recognized case of KHV occurred in the United Kingdom in 1996 (Haenen et al. 2004). Since then other cases have been confirmed in almost all countries that culture koi and/ or common carp with the exception of Australia (Hedrick et al. 2000; Haenen et al. 2004, Pokorova et al. 2005). This information sheet is intended to inform veterinarians, biologists, fish producers and hobbyists about KHV disease. What Is KHV? Figure 1. Koi with mottled gills and sunken eyes due to koi Koi herpesvirus (also known as Cyprinid herpesvirus 3; herpesvirus disease. Credit: Deborah B. Pouder, University of Florida CyHV3) is classified as a double-stranded DNA virus herpesvirus, based on virus morphology and genetics, and belonging to the family Alloherpesviridae (which includes is closely related to carp pox virus (Cyprinid herpesvirus fish herpesviruses). The work of Waltzek and colleagues 1; CyHV1) and goldfish hematopoietic necrosis virus (Waltzek et al. 2005, 2009) revealed that KHV is indeed a (Cyprinid herpesvirus 2; CyHV2). Koi herpesvirus disease has been diagnosed in koi and common carp (Hedrick 1.
  • Identification and Characterization of Cyprinid Herpesvirus-3 (Cyhv-3) Encoded Micrornas

    Identification and Characterization of Cyprinid Herpesvirus-3 (Cyhv-3) Encoded Micrornas

    RESEARCH ARTICLE Identification and Characterization of Cyprinid Herpesvirus-3 (CyHV-3) Encoded MicroRNAs Owen H. Donohoe1,2, Kathy Henshilwood1, Keith Way3, Roya Hakimjavadi2, David M. Stone3, Dermot Walls2* 1 Marine Institute, Rinville, Oranmore, Co. Galway, Ireland, 2 School of Biotechnology and National Centre for Sensor Research, Dublin City University, Dublin, Ireland, 3 Centre for Environment, Fisheries and Aquaculture Science (Cefas), The Nothe, Weymouth, Dorset, the United Kingdom a11111 * [email protected] Abstract MicroRNAs (miRNAs) are a class of small non-coding RNAs involved in post-transcriptional OPEN ACCESS gene regulation. Some viruses encode their own miRNAs and these are increasingly being Citation: Donohoe OH, Henshilwood K, Way K, recognized as important modulators of viral and host gene expression. Cyprinid herpesvirus Hakimjavadi R, Stone DM, Walls D (2015) 3 (CyHV-3) is a highly pathogenic agent that causes acute mass mortalities in carp (Cyprinus Identification and Characterization of Cyprinid Herpesvirus-3 (CyHV-3) Encoded MicroRNAs. PLoS carpio carpio) and koi (Cyprinus carpio koi) worldwide. Here, bioinformatic analyses of the ONE 10(4): e0125434. doi:10.1371/journal. CyHV-3 genome suggested the presence of non-conserved precursor miRNA (pre-miRNA) pone.0125434 genes. Deep sequencing of small RNA fractions prepared from in vitro CyHV-3 infections led Academic Editor: Sebastien Pfeffer, French National to the identification of potential miRNAs and miRNA–offset RNAs (moRNAs) derived from Center for Scientific Research - Institut de biologie some bioinformatically predicted pre-miRNAs. DNA microarray hybridization analysis, North- moléculaire et cellulaire, FRANCE ern blotting and stem-loop RT-qPCR were then used to definitively confirm that CyHV-3 Received: December 15, 2014 expresses two pre-miRNAs during infection in vitro.
  • Shedding of the Salmonid Herpesvirus-3 by Infected Lake Trout (Salvelinus Namaycush)

    Shedding of the Salmonid Herpesvirus-3 by Infected Lake Trout (Salvelinus Namaycush)

    viruses Communication Shedding of the Salmonid Herpesvirus-3 by Infected Lake Trout (Salvelinus namaycush) Mohamed Faisal 1,2,3, Mochamad Purbayu 3, Megan A. Shavalier 2,3, Terence L. Marsh 4 and Thomas P. Loch 1,2,* 1 Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA 2 Department of Fisheries and Wildlife, College of Agriculture and Natural Resources, Michigan State University, East Lansing, MI 48824, USA 3 Comparative Medicine and Integrative Biology, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA 4 Department of Microbiology and Molecular Genetics, College of Natural Science, Michigan State University, East Lansing, MI 48824, USA * Correspondence: [email protected]; Tel.: +517-884-2019; Fax: +517-432-2310 Received: 9 May 2019; Accepted: 20 June 2019; Published: 26 June 2019 Abstract: Salmonid Herpesvirus-3, commonly known as the Epizootic Epitheliotropic Disease virus (EEDV), causes a disease of lake trout (Salvelinus namaycush) that has killed millions of fish over the past several decades. Currently, most aspects of EEDV disease ecology remain unknown. In this study, we investigated EEDV shedding in experimentally challenged (intracoelomic injection) lake trout that were individually microchipped. In order to assess viral shedding, each infected fish was placed in individual static, aerated aquaria for a period of 8 h, after which the water was assessed for the presence of EEDV DNA using quantitative PCR. Water sampling was conducted every seven days for 93 days post-infection (pi), followed by additional sampling after one year. Results demonstrated that lake trout began shedding EEDV into the water as early as 9 days pi.