Clean Room Use, Liquid Nitrogen Storage Facility and Cell
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SUPPLY OF CELL LINES FOR RESEARCH PURPOSE Department of Biotechnology Following are the other advantages of the Government of India system: Cell lines available with TRPVB will be provided to academic institutions for research © Completely dry storage area use with a nominal charge. A purchase CLEAN ROOM USE, © Warmest temperature of -190°C at vapour agreement will be signed by the purchasing LIQUID NITROGEN phase institution for using the cells for non-commercial STORAGE FACILITY AND purpose. The cells will be provided with © Microprocessor controlled automatic CELL LINE SUPPLY monitoring and alarms for deviations from mycoplasma-free, bacterial and fungal the set values of levels and temperatures contamination-free certification. Additional charge will be levied for getting bovine and © Automatic maintenance of LN2 level porcine adventitious virus-free status for the cell Cell lines, hybridomas and protein lines. Following cell lines are available for expressing clones of mammalian cells can be purchase stored in the facility as an alternate storage 1. Vero cells facility to avoid the loss of clones. 2. BHK 21 monolayer cells WHO CAN STORE 3. A72 cells The LN storage system can be used as cell 2 4. Sf9 cells repository by various departments in TANUVAS, other institutions and needy researcher / 5. MDBK cells entrepreneurs, provided the cells are certified / 6. MDCK cells proven to be free from bacteria, fungaus, mycoplasma and other relevant adventitious 7. HEK 293 T cells viral agents. TERMS AND CONDITIONS FOR STORAGE CONTACT Revival and survival of cells depend on various factors including the status of cells The Project Director during cryo-preservation, cryo-preservation procedure and storage until the material is Translational Research Platform for Veterinary Biologicals nd transferred to TRPVB. TRPVB has the right to 2 Floor, Central University Laboratory (CUL) Building, CAHS, check the cells randomly for adventitious TANUVAS, Madhavaram Milk Colony, Chennai – 600 051 agents without informing the faculty. If the cells E mail : [email protected]; Translational Research Platform for Veterinary Biologicals nd are found positive for the adventitious agents, Phone – 044 – 25556275 – 77 2 Floor, Central University Laboratory (CUL) Building the cells will be discarded. Centre for Animal Health Studies Tamil Nadu Veterinary and Animal Sciences University (TANUVAS) Madhavaram Milk Colony, Chennai - 600 051 Cost for the above services will be provided on request www.trpvb.org.in ABOUT TRPVB seed development and other testing © The facility can be utilized for process TRPVB is a unique partnership programme procedures at their cGMP certified facility as per optimization at pilot scale between Department of Biotechnology and the stipulated regulatory norms, and these © The facility can be utilized as a repository for Tamil Nadu Veterinary and Animal Sciences processes often take years to complete. To fill cGMP vaccine seeds and cell banks this gap and also to facilitate seamless transfer University in the field of Translational Research LN cRYO STORAGE FACILITY FOR CELLs for Veterinary Biologicals. The research team of the technologies from the academia to 2 includes an amalgamated combination of industry, TRPVB-TANUVAS had created a state Mammalian cell lines, clones and academia / industry and regulatory experts who of the art clean room facility meeting cGMP hybridomas with potential application in can leverage and assist clients in various stages norms (ISO class 7 and class 8 air research and commercialisation are being of product development. It is housed in one of classifications). developed by various research organisations. the leading Veterinary Universities in India © The facility is available for use by interested These clones are cryo-preserved in LN2 for long which provides the much needed academic industry, institutions, entrepreneurs and time storage and usage. The TRPVB's clean ambience to act as a knowledge hub for start-up companies on payment basis room facility is equipped with a mammalian cell information resources. line storage facility. The LN freezer system has © The facility can be utilized for a specific 2 CLEAN ROOM FACILITY purpose for a particular duration a capacity to store 15,600 numbers of 2 ml vials in vapour phase. The storage system is with Academic institutions in India including PROPOSED UTILITY clearly sorted racks and boxes at a vapour TANUVAS is focusing on the development of © cGMP grade vaccine seeds and vaccine phase. This freezer is attached with TEC 3000 newer and better diagnostic and control substrates (cell lines) can be produced with Controller that enables automated monitoring of measures against the infectious diseases of the requisite QC testing and documentation LN2 levels and temperature. The system is animals. Though the academic institutions of as per the regulatory norms. These seeds attached with a 230 lt LN2 storage container from India possess adequate scientific acumen to can be used directly as vaccine banks which the LN level in the cryovial storage develop the technologies, unavailability / 2 anywhere in the world container is automatically inaccessibility to the cGMP structural maintained. requirements impede them in meeting the © cGMP grade test batches (intended for quality standards. This impose additional clinical trials) can be produced with the burden on the manufacturing companies which requisite QC testing and documentation as acquire the technology from any academic per the regulatory norms institution. The companies need to repeat the ABOUT TRPVB repeat the seed development and other testing © The facility can be utilized for process procedures at their cGMP certified facility as per optimization at pilot scale TRPVB is a unique partnership programme between Department of Biotechnology and Tamil the stipulated regulatory norms, and these © The facility can be utilized as a repository for Nadu Veterinary and Animal Sciences University processes often take years to complete. To fill this cGMP vaccine seeds and cell banks gap and also to facilitate seamless transfer of the in the field of Translational Research for Veterinary LN cRYO STORAGE FACILITY FOR CELLs Biologicals. The research team includes an technologies from the academia to industry, 2 amalgamated combination of academia / industry TRPVB-TANUVAS had created a state of the art Mammalian cell lines, clones and hybridomas and regulatory experts who can leverage and clean room facility meeting cGMP norms (ISO with potential application in research and assist clients in various stages of product class 7 and class 8 air classifications). commercialisation are being developed by development. It is housed in one of the leading © The facility is available for use by interested various research organisations. These clones are Veterinary Universities in India which provides the cryo-preserved in LN for long time storage and much needed academic ambience to act as a industry, institutions, entrepreneurs and start- 2 knowledge hub for information resources. up companies on payment basis usage. The TRPVB's clean room facility is equipped with a mammalian cell line storage © The facility can be utilized for a specific CLEAN ROOM FACILITY facility. The LN freezer system has a capacity to purpose for a particular duration 2 Academic institutions in India including store 15,600 numbers of 2 ml vials in vapour TANUVAS is focusing on the development of PROPOSED UTILITY phase. The storage system is with clearly sorted newer and better diagnostic and control measures © cGMP grade vaccine seeds and vaccine racks and boxes at a vapour phase. This freezer is against the infectious diseases of animals. Though substrates (cell lines) can be produced with attached with TEC 3000 Controller that enables the academic institutions of India possess the requisite QC testing and documentation as automated monitoring of LN2 levels and adequate scientific acumen to develop the per the regulatory norms. These seeds can be temperature. The system is attached with a 230 lt technologies, unavailability / inaccessibility to the used directly as vaccine banks anywhere in LN2 storage container from which the LN2 level in cGMP structural requirements impede them in the world the cryovial storage container is meeting the quality standards. This impose automatically maintained. © cGMP grade test batches (intended for clinical additional burden on the manufacturing trials) can be produced with the requisite QC companies which acquire the technology from any testing and documentation as per the academic institution. The companies need to regulatory norms.