ROCHE PRODUCTS for Distribution by Sigma-Aldrich

Total Page:16

File Type:pdf, Size:1020Kb

ROCHE PRODUCTS for Distribution by Sigma-Aldrich ROCHE PRODUCTS For Distribution by Sigma-Aldrich Researchers will soon be able to turn to Sigma-Aldrich as an Sigma-Aldrich and Roche are currently working on processes to ensure a seamless transition for customers. It is expected that easy, single source supplier of well-respected, high quality Roche products will be available to purchase through the award- winning Sigma-Aldrich website in mid-2015. Until then, customers genomics, proteomics and cell analysis reagents and kits should continue to purchase products through their standard from Roche. Roche channels. The products listed below are complementary to Sigma-Aldrich's biology offer and industry-leading levels of service. Together, we will provide researchers with global access to key materials across For more information, visit their workflow. sigmaaldrich.com/roche Roche Product List Cat. No. Product Description Cat. No. Product Description 10148334001 2.3-Diphosphoglycerate Test kit 10127558001 ADH, susp. 1g (34ml) 11051466001 2'-Deoxy-guanosine-5'-TP Solution 11685660001 Agarose LE, 100g 10127833001 3-HBDH, Grade II 11685678001 Agarose LE, 500g 10127841001 3-HBDH, Grade II 11388983001 Agarose MP, 100g 03353621001 5'/3' RACE Kit, 2nd generation 11388991001 Agarose MP, 500g 11296736001 5-Bromo-2´-dU Labeling/Detection Kit I 11816586001 Agarose MS, 100 g 11299964001 5-Bromo-2´-dU Labeling/Detection Kit II 11816594001 Agarose MS, 500 g 11444611001 5-Bromo-2´-dU Labeling/Detection Kit III 10171832001 Aldehyde Dehydrogenase from Yeast 10280879001 5-Bromo-2'-deoxy-uridine 03359123001 Alkaline Phosphatase, recomb. 10988537001 7-Deaza-2'-deoxy-GTP, Di-Li 10602442001 alpha-2 -Macroglobulin 10102946001 ABTS 10102814001 alpha-Amylase (pig pancreas) 11204530001 ABTS Buffer, 125ml 11742027001 alpha-Glucosidase Assay 1pc 11112597001 ABTS Buffer, 16.7g 10835269001 Ampicillin, 50g 11684302001 ABTS Solution 10835242001 Ampicillin, 5g 11204521001 ABTS Tablets, 5 mg 10102857001 Amyloglucosidase from Aspergillus niger 11112422001 ABTS Tablets, 50 mg 11202367001 Amyloglucosidase from Aspergillus niger 10101893001 Acetyl-CoA, 10mg 11202332001 Amyloglucosidase, Lyo., SQ (500 U) 11585371001 Acetyl-CoA, 200mg 11858777001 Annexin V FLUOS Staining Kit 10101907001 Acetyl-CoA, 50mg 11828681001 Annexin-V-FLUOS 11498045910 Actin RNA Probe, DIG 2ug 11988549001 Annexin-V-Fluos Staining Kit 10102105001 Adenosine Deaminase (ADA), 10mg 11170376001 Anti-BRDU, Formalin Grade 10102121001 Adenosine Deaminase (ADA), susp. 11202693001 Anti-BRDU-Flourescein, Formalin Grade 10102547001 Adenylyl-imidodiphosphate (AMP-PNP), Li4 11585860001 Anti-BRDU-Peroxidase, Formalin Grade Cat. No. Product Description Cat. No. Product Description 11667149001 Anti-c-myc, 200 ug 11539426001 b-Gal ELISA 11667203001 Anti-c-myc, 5 mg 11758241001 b-Gal Reporter Gene Assay, chemilum. 11814150001 Anti-c-myc-Peroxidase 11828673001 b-Gal Staining Set 11093274910 Anti-digoxigenin AP-conjugate 10662046001 b-Galactose Dehydrogenase S 11333062910 Anti-digoxigenin MAB 10104973001 b-Galactose Dehydrogenase, 1mg 11207733910 Anti-digoxigenin POD-conjugate 10104981001 b-Galactose Dehydrogenase, 5mg 11214667001 Anti-Digoxigenin, Fab Fragments, Sheep 10105031001 b-Galactosidase, 1500U 11333089001 Anti-Digoxigenin, Sheep 10745731001 b-Galactosidase, 25mg 11207741910 Anti-digoxigenin-fluorescein 03707601001 b-Glucuronidase, 15ml 11633716001 Anti-Digoxigenin-POD (poly) 03707580001 b-Glucuronidase, 1ml 11207750910 Anti-digoxigenin-rhodamine 03707598001 b-Glucuronidase, 5ml 11426320001 Anti-Fluorescein 10127698001 b-Glucuronidase/Arylsulfatase, 10ml 11426338910 Anti-fluorescein-ap Fab Fragments 10127060001 b-Glucuronidase/Arylsulfatase, 2ml 11426346910 Anti-Fluorescein-POD 11585649910 Biotin High Prime 100ul 11814460001 Anti-GFP 11418165001 Biotin Protein Labeling Kit 11583816001 Anti-HA (12CA5), 200ug 11685597910 Biotin RNA Labeling Mix 10x 11666606001 Anti-HA (12CA5), 5mg 04739205001 Biotin-11-CTP 11815016001 Anti-HA Affinity Matrix 11427598910 Biotin-16-ddUTP 11666851001 Anti-HA Biotin 11093070910 Biotin-16-dUTP, Solution 11666878001 Anti-HA Fluorescein 11388908910 Biotin-16-UTP 11867431001 Anti-HA High Affinity (3F10), 500ug 11745824910 Biotin-Nick translat.mix f.in 11867423001 Anti-HA High Affinity (3F10), 50ug 11096176001 Blocking Reagent 50 g 12158167001 Anti-HA-Biotin, High Affinity (3F10) 11112589001 Blocking Reagent for ELISA 11988506001 Anti-HA-Fluorescein, High Affinity 11975188001 Blue Genes Reagent Set 11667475001 Anti-HA-Peroxidase 11442066001 BM Blue POD Substrate, precipitating 12013819001 Anti-HA-Peroxidase, High Affinity (3F10) 11484281001 BM Blue POD Substrate, soluble 11922416001 Anti-His_6 11759779001 BM Chem-Lum. ELISA Substr. (AP) 11965085001 Anti-His_6 -Peroxidase 11582950001 BM Chem-Lum. ELISA Substr. (POD) 11004646001 Antipain dihydrochloride 11520709001 BM Chem-Lum. WB-Kit (Mouse/Rabbit) 11835238001 Anti-Poly (ADP-Ribose) Polymerase 11500708001 BM Chem-Lum. WB-Substr. (POD) 1000 11815024001 Anti-Protein C Affinity Matrix 11500694001 BM Chem-Lum. WB-Substr. (POD) 4000 11835246001 Apoptotic DNA-Ladder Kit 10663573001 BM Condimed 11583794001 Aprotinin, 100mg 11088947001 BM Condimed H1 (Hyb.-Cloning-Suppl.) 10236624001 Aprotinin, 10mg 10799050001 BM Cyclin 10981532001 Aprotinin, 50mg 11442074001 BM Purple AP Substrate, precipitating 06879110001 AptaTaq Fast DNA Polymerase, 100 Units 03117057001 BSA FATTY ACID FREE 100 GRAMS 06879128001 AptaTaq Fast DNA Polymerase, 1000 Units 03116964001 BSA FRACTION V 1 KG 06879080001 AptaTaq Fast PCR Master, 100 Reactions 03116956001 BSA FRACTION V 250 GRAMS 06879101001 AptaTaq Fast PCR Master, 1000 Reactions 03117332001 BSA FRACTION V CE PROT FREE 50 G 10736619001 Ascorbate Oxidase Spatula 10735086001 BSA Fraction V, 100g 11699695001 ATP Bioluminescence Assay Kit CLS II 10735108001 BSA Fraction V, 1kg 11699709001 ATP Bioluminescence Assay Kit HS II 10735094001 BSA Fraction V, 500g 10127531001 ATP, disodium salt, 10g 10735078001 BSA Fraction V, 50g 10127523001 ATP, disodium salt, 5g 10775835001 BSA Fraction V, Fatty Acid free, 50g 10519979001 ATP, disodium salt, special quality, 1g 10711454001 BSA, 20mg, SQ for Molecular Biology 10519987001 ATP, disodium salt, special quality, 5g 11666789001 Buffers in a Box, premix PBS Buffer(10x) 11140965001 ATP, lithium salt 11666681001 Buffers in a Box, premix SSC Buffer(20x) 10102342001 ATP-gamma-S, Powder 11666690001 Buffers in a Box, premix TAE Buffer(10x) 11162306001 ATP-gamma-S, Solution 11086090001 Calpain Inhibitor I (25 MG) 11681982001 AttoPhos Substrat Set 10103233001 Carboxypeptidase B 11104616001 bbFGF (156AA) 10103241001 Carnitine Acetyltransferase 11585002001 BCIP, 4-toluidine salt, powder, 1g 11363727001 CAT ELISA 10760994001 BCIP, 4-toluidine salt, powder, 250mg 11685627001 CDP-Star 1 ml 11383221001 BCIP, 4-toluidine salt, solution 11759051001 CDP-Star 2 x 1 ml 10874515001 Bestatin, 10mg 12041677001 CDP-Star, ready-to-use Cat. No. Product Description Cat. No. Product Description 11544675001 Cell Death Detection ELISA 05892970001 Complete ULTRA, mini, EASYpack 11774425001 Cell Death Detection ELISA^PLUS 05892791001 Complete ULTRA, mini, EDTA-free EASYpack 11920685001 Cell Death Detection ELISA^PLUS, 10 x 11697498001 Complete, 20 tablets 11669915001 Cell Proliferation ELISA, BrdU chem.lum. 11836145001 Complete, 3 x 20 tablets 11647229001 Cell Proliferation ELISA, BrdU (colorim.) 11873580001 Complete, EDTA-free, 20 tablets 11465007001 Cell Proliferation Kit I (MTT) 04693116001 Complete, EASYpack, 20 Tab. 11465015001 Cell Proliferation Kit II (XTT) 04693132001 Complete, EDTA-free, EASYpack, 20 Tab. 11644807001 Cell Proliferation Reagent WST-1 04693124001 Complete, Mini, EASYpack, 30 Tab. 05015944001 Cell Proliferation Reagent WST-1, 8 ml 04693159001 Complete, Mini, EDTA-free, EASYpack, 30 Tab. 06432379001 Cell Viability Imaging Kit 5x96 11581074001 COT Human DNA 11585045001 Cellular DNA Fragmentation ELISA 05480647001 COT Human DNA, fluorometric grade 10810118001 CHAPS, 10g 10884308001 CPRG 10810126001 CHAPS, 50g 10127566001 Creatine Kinase (CK), 100mg 10378445001 Chromozym PK 10736988001 Creatine Kinase (CK), 500mg 10378461001 Chromozym PL 20 mg 10621722001 Creatine phosphate, 10g 10206849001 Chromozym TH 10621714001 Creatine phosphate, 5g 11585398001 Chromozym TH 100 mg 11655884001 CSPD, 1ml 11093037001 Chromozym t-PA 11755633001 CSPD, ready-to-use, 100ml 11004638001 Chymostatin 11140922001 CTP, lithium salt 11418467001 Chymotrypsin Sequencing Grade 04744926001 Cytotoxicity Det.Kit PLUS (LDH) 400tests 10354074001 Citrate Lyase (CL) 04744934001 Cytotoxicity Det.Kit PLUS(LDH) 2000tests 10295892001 Colcemid 11644793001 Cytotoxicity Detection Kit (LDH) 11179179001 Collagen, cell culture grade 11626353001 D(-)-Luciferin, 50mg 10103578001 Collagenase A, 100mg 11718096001 DAB Substrate 11088793001 Collagenase A, 2.5g, non-sterile 10236276001 DAPI 10103586001 Collagenase A, 500mg 11051440001 dATP 11088807001 Collagenase B, 100mg 11934511001 dATP,PCR Grade Di-Na slt 11088831001 Collagenase B, 2.5g, non-sterile 11969013001 dATP,PCR Grade Di-Na solution 11088815001 Collagenase B, 500mg 03732681001 dATP,PCR Grade Di-Na Solution 4x125 µM 11088858001 Collagenase D, 100mg 11051458001 dCTP 11088882001 Collagenase D, 2.5g, non-sterile 11934520001 dCTP,PCR Grade Di-Na slt 11088866001 Collagenase D, 500mg 11969021001 dCTP,PCR Grade Di-Na solution 11074032001 Collagenase H, 100mg 03732690001 dCTP,PCR Grade Di-Na Solution 4x125 µM 11087789001 Collagenase H, 2.5g, non-sterile 03732738001 ddNTP Set, Sequencing Grade 11074059001 Collagenase H, 500mg 03622614001
Recommended publications
  • Expand Reverse Transcriptase from Escherichia Coli: AP401 (K) Deoxynucleoside-Triphosphate:DNA Deoxynucleotidyl-Transferase (RNA Directed) E.C.2.7.7.49
    For life science research only. Not for use in diagnostic procedures. Expand Reverse Transcriptase from Escherichia coli: AP401 (k) Deoxynucleoside-triphosphate:DNA deoxynucleotidyl-transferase (RNA directed) E.C.2.7.7.49 Cat. No. 11 785 826 001 1 000 U y Version 13 Cat. No. 11 785 834 001 5 000 U Content version: March 2020 Store at Ϫ15 to Ϫ25°C Product overview General handling instructions Pack content RNA preparation For high quality eucaryotic mRNA preparations it is Vial Content necessary to minimize the activity of RNases liberated Expand Reverse • 1000 U (Cat. No. 11 785 826 001) during cell lysis by using inhibitors of RNases or meth- ods that disrupt cells and inactivate RNases simultane- Transcriptase • 5000 U (Cat. No. 11 785 834 001) ously. A good overview of the methods is given in (4) as Expand Reverse cDNA synthesis buffer (first-strand) well as in (5). Suitable reagents for the isolation of total Transcriptase 250 mM Tris-HCl, 200 mM KCl, 2) RNA/or mRNA are the mRNA Isolation Kit and TriPure Buffer, 5 × conc. 25 mM MgCl2, 2.5% Tween 20 Isolation Reagent. (v/v), pH 8.3 (25°C). Consequently, it is also important to avoid the acciden- Dithiothreitol 100 mM tal introduction of trace amounts of RNases from other (DTT) solution potential sources in the laboratory, like glassware, plasticware, contaminating solutions and contamina- Storage and The undiluted enzyme is stable at –15 to –25°C through tion of the investigators hands. stability the expiration date printed on the label. The product is shipped on dry ice.
    [Show full text]
  • For Improvement of Nucleic Acid Synthesis and Ampli
    Europäisches Patentamt *EP001088891B1* (19) European Patent Office Office européen des brevets (11) EP 1 088 891 B1 (12) EUROPEAN PATENT SPECIFICATION (45) Date of publication and mention (51) Int Cl.7: C12N 15/55, C12N 15/54, of the grant of the patent: C12N 9/22, C12N 9/12, 12.01.2005 Bulletin 2005/02 C12Q 1/68, C12P 19/34 (21) Application number: 99119268.3 (22) Date of filing: 28.09.1999 (54) Thermostable enzyme promoting the fidelity of thermostable DNA polymerases - for improvement of nucleic acid synthesis and amplification in vitro Thermostabiles Enzym welches die Genauigkeit thermostabiler DNA Polymerasen erhöht - zur Verbesserung der Nucleinsäuresynthese und in vitro Amplifikation Enzyme thermostable pour augmenter la fidélité de polymèrase d’ADN thermostable - pour l’amélioration de la synthèse des acides nucléiques et d’amplification in vitro (84) Designated Contracting States: • KLENK H-P ET AL: "The complete genome AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU sequence of the hyperthermophilic, MC NL PT SE sulphate-reducing archaeon Archaeoglobus fulgidus" NATURE,GB,MACMILLAN JOURNALS (43) Date of publication of application: LTD. LONDON, vol. 390, 27 November 1997 04.04.2001 Bulletin 2001/14 (1997-11-27), pages 364-370, XP002091622 ISSN: 0028-0836 (73) Proprietor: Roche Diagnostics GmbH • KALUZ S ET AL: "DIRECTIONAL CLONING OF 68298 Mannheim (DE) PCR PRODUCTS USING EXONUCLEASE III" NUCLEIC ACIDS RESEARCH,GB,OXFORD (72) Inventors: UNIVERSITY PRESS, SURREY, vol. 20, no. 16, 1 • Dr.Waltraud Ankenbauer January 1992 (1992-01-01), pages 4369-4370, 82377 Penzberg (DE) XP002072726 ISSN: 0305-1048 • Franck Laue • BOOTH P M ET AL: "ASSEMBLY AND CLONING 82396 Paehl-Fischen (DE) OF CODING SEQUENCES FOR NEUROTROPHIC • Dr.Harald Sobek FACTORS DIRECTLY FROM GENOMIC DNA 82377 Penzberg (DE) USING POLYMERASE CHAIN REACTION AND • Michael Greif URACIL DNA GLYCOSYLASE" 83661 Lenggries (DE) GENE,NL,ELSEVIER BIOMEDICAL PRESS.
    [Show full text]
  • (10) Patent No.: US 8715987 B2
    US008715987B2 (12) United States Patent (10) Patent No.: US 8,715,987 B2 Johnson et al. (45) Date of Patent: May 6, 2014 (54) SOLUBILIZED PHOSPHOLIPIDS FOR WO WOO 132887 A1 5, 2001 STABILIZING NUCLECACID WO WO2O080 13885 A2 1, 2008 POLYMERASES WO WO2O08077O17 A2 6, 2008 OTHER PUBLICATIONS (75) Inventors: Donald Johnson, Brookline, MA (US); Thomas C. Evans, Jr., Topsfield, MA Manual Translation CN 101570792A, Nov. 2009.* Barnes, WM. “The fidelity of Taq polymerase catalyzing PCR is (US) improved by an N-terminal deletion”. Gene, 112(1):29-35, 1992. Cann, et al., “A heterodimeric DNA polymerase: evidence that mem (73) Assignee: New England Biolabs, Inc., Ipswich, bers of Euryarchaeota possess a distinct DNA polymerase.”. Proc. MA (US) Natl. Acad. Sci. USA,95: 14250-5, 1998. Capitani.etal, “Effect of phosphatidylcholine vesicles on the activity (*) Notice: Subject to any disclaimer, the term of this of DNA polymerase-O.", Mol and Cell Biochem 27:137-138, 1979. patent is extended or adjusted under 35 Chien, et al., “Deoxyribonucleic acid polymerase from the extreme U.S.C. 154(b) by 0 days. thermophile Thermus aquaticus”,Journal of Bacteriology, 127:1550, 1976. Diaz, et al., "Accuracy of replication in the polymerase chain reaction. (21) Appl. No.: 13/450,549 Comparison between Thermotoga maritima DNA polymerase and Thermus aquaticus DNA polymerase.”. Brazilian Journal of Medical (22) Filed: Apr. 19, 2012 and Biological Research, 31:1239, 1998. Eckert , et al. “The Fidelity of DNA Polymerases used in the (65) Prior Publication Data polymerase chain reactions'. Oxford University Press, New York, US 2012/O282669 A1 Nov.
    [Show full text]
  • Pwo DNA Polymerase Deoxynucleoside-Triphosphate: DNA Deoxynucleotidyl-Transferase (DNA-Directed); EC 2.7.7.7
    Version 060918 G:\products\productflyer\pcr\polymerasen\proof\descr_m3002_pwo_en.docx ENAXXON fon: +49(0)731 – 3608 123 X G fax: +49(0)731 – 3608 962 b i o s c i e n c e E-Mail: [email protected] Pwo DNA Polymerase Deoxynucleoside-triphosphate: DNA deoxynucleotidyl-transferase (DNA-directed); EC 2.7.7.7 Product Cat# Package size Pwo DNA Polymerase (proof-reading polymerase) M3002.0100 100 units Pwo DNA Polymerase (proof-reading polymerase) M3002.0500 2 x 250 units Pwo DNA Polymerase (proof-reading polymerase) M3002.1250 5 x 250 units Description The Genaxxon bioscience Pwo DNA Polymerase is a thermostable enzyme possessing 5‘-3‘ DNA polymerase and 3‘-5‘ proof reading exonuclease activities. It is isolated from the hyperthermophilic marine archae Pyroccocus woesei (Pwo). The enzyme provides extremely high fidelity. Whereas the enzyme is not able to amplify long fragments as efficiently as the Genaxxon Taq polymerase because of its very high exonuclease activity. To overcome this restriction we recommend to use the Genaxxon ReproFast DNA Polymerase, which will provide a more robust synthesis of longer amplification products (Barnes et al. (1994) Proc. Natl. Acad. Sci., USA 91, 2216-2220). Use of the Genaxxon Pwo DNA Polymerase in amplification results in blunt-ended products, which is not recommended for cloning into T/A vectors. Concentration: 2.5 units/µL Unit definition: One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid-insoluble form in 30 min at 72°C under the assay conditions (25mM TAPS (tris-(hydroxymethyl)methyl-amino-propane-sulphonic acid, sodium salt) pH9.3 (at 25°C), 50mM KCl, 2mM MgCl2, 1mM ß-mercaptoethanol) and activated calf thymus DNA as substrate.
    [Show full text]
  • Taq DNA Polymerase
    Polymerase Chain Reaction (PCR) • PCR is a technique which is used to amplify the number of copies of a specific region of DNA, in order to produce enough DNA to be adequately tested. • As a result, it now becomes possible to analyze and characterize DNA fragments found in minute quantities in different samples. Polymerase Chain Reaction (PCR) • PCR carry out for – DNA cloning – sequencing, – DNA-based phylogeny, or – functional analysis of genes; – the diagnosis of diseases; – genetic fingerprints and – the detection and diagnosis of infectious diseases. PROCEDURE ….. PCR Reagents • Template DNA (e.g., plasmid DNA, genomic DNA). • Forward and reverse PCR primers. • MgCl2 (25 mM). • dNTPs (a mixture of 2.5 mM dATP, dCTP, dGTP, and dTTP). • PCR buffer: 500 mM KCl, 100 mM Tris-HCl, pH 8.3, 25°C. PCR Reagents • Thermal stable DNA polymerase – DNA polymerases without 3′ → 5′ proofreading activity • Taq DNA polymerase • Tth DNA polymerase • Tfl DNA polymerase – DNA polymerases with 3′ → 5′ proofreading activity: • Pwo DNA polymerase • Pfu DNA polymerase • Tli DNA polymerase • Vent DNA polymerase PCR Reagents • Optional: PCR additives /cosolvents (optional; e.g., • betaine, glycerol, DMSO, formamide, bovine serum albumin, ammonium sulfate, polyethylene glycol, gelatin, Tween-20, Triton X-100, β-mercaptoethanol, or tetramethylammonium chloride). Polymerase Chain Reaction (PCR) • Initialization step: 94–96 °C (or 98 °C if extremely thermostable polymerases are used), for 1–9 minutes. – the complete separation of the DNA strands and unfolding secondary structures – This stage also required for DNA polymerases that require heat activation by hot-start PCR. Polymerase Chain Reaction (PCR) • Denaturation step: 94–98 °C for 20–30 seconds.
    [Show full text]
  • Roche Applied Science
    FNR498_PCR_RZ 02.06.2006 14:55 Uhr Seite 1 C M Y CM MY CY CMY K PCR Applications Manual 3rd edition www.roche-applied-science.com Probedruck Acknowledgement We would like to thank all contributors and editors for their diligent efforts. Without their work, this project would not have been possible. Impressum Editorial Management: Degen, Hans-Joachim, Ph.D. Deufel, Annette, Ph.D. Eisel, Doris Grünewald-Janho, Stefanie Keesey, Joe, Ph.D. Art Direction: Fanz & Neumayer, Schifferstadt Layout Design and Typesetting: Active Artware GmbH, Saarbrücken © 2006 by Roche Diagnostics GmbH, Mannheim All rights reserved. No part of this booklet may be reproduced in any form without written permission of the publishers. Printed in Germany. www.roche-applied-science.com Table of Contents 1 Introduction Page 1.1 Principles of PCR and RT-PCR ........................................................................................................................... 9 1.2 The Evolution of PCR ..........................................................................................................................................11 1.3 Purpose of this PCR Applications Manual...................................................................................................15 2 General Guidelines Page 2.1 Preventing Contamination in the PCR Laboratory....................................................................................19 2.1.1 Setting Up the PCR Laboratory Space Correctly.......................................................................................20
    [Show full text]
  • Sigma-Aldrich Biochemical Reagents
    Expanding access to our Biochemical Reagent products New distribution agreement with Sigma-Aldrich Material No. SKU name Material No. SKU name 10148334001 2.3-Diphosphoglycerate Test Kit 11816594001 Agarose MS, 500 g 11051466001 2’-Deoxy-guanosine-5’-TP Solution 10171832001 Aldehyde Dehydrogenase from Yeast 10127833001 3-HBDH, Grade II 03359123001 Alkaline Phosphatase, recomb. 10127841001 3-HBDH, Grade II 10602442001 alpha-2 -Macroglobulin 03353621001 5’/3’ RACE Kit, 2nd generation 10102814001 alpha-Amylase (pig pancreas) 11296736001 5-Bromo-2´-dU Labeling/Detection Kit I 11742027001 alpha-Glucosidase Assay, 1 pc 11299964001 5-Bromo-2´-dU Labeling/Detection Kit II 10835269001 Ampicillin, 50 g 11444611001 5-Bromo-2´-dU Labeling/Detection Kit III 10835242001 Ampicillin, 5 g 10280879001 5-Bromo-2’-deoxy-uridine 10102857001 Amyloglucosidase from Aspergillus niger 10988537001 7-Deaza-2’-deoxy-GTP, Di-Li 11202367001 Amyloglucosidase from Aspergillus niger 10102946001 ABTS 11202332001 Amyloglucosidase, Lyo., SQ (500 U) 11204530001 ABTS Buffer, 125 ml 11858777001 Annexin V Fluos Staining Kit 11112597001 ABTS Buffer, 16.7 g 11828681001 Annexin-V-Fluos 11684302001 ABTS Solution 11988549001 Annexin-V-Fluos Staining Kit 11204521001 ABTS Tablets, 5 mg 11170376001 Anti-BRDU, Formalin Grade 11112422001 ABTS Tablets, 50 mg 11202693001 Anti-BRDU-Flourescein, Formalin Grade 10101893001 Acetyl-CoA, 10 mg 11585860001 Anti-BRDU-Peroxidase, Formalin Grade 11585371001 Acetyl-CoA, 200 mg 11667149001 Anti-c-myc, 200 ug 10101907001 Acetyl-CoA, 50 mg 11667203001 Anti-c-myc, 5 mg 11498045910 Actin RNA Probe, DIG, 2 ug 11814150001 Anti-c-myc-Peroxidase 10102105001 Adenosine Deaminase (ADA), 10 mg 11093274910 Anti-Digoxigenin AP-conjugate 10102121001 Adenosine Deaminase (ADA), susp. 11333062910 Anti-Digoxigenin MAB 10102547001 Adenylyl-imidodiphosphate (AMP-PNP), Li4 11207733910 Anti-Digoxigenin POD-conjugate 10127558001 ADH, susp.
    [Show full text]
  • Overview of Thermostable DNA Polymerases for Classical PCR Applications: from Molecular and Biochemical Fundamentals to Commercial Systems
    Appl Microbiol Biotechnol (2013) 97:10243–10254 DOI 10.1007/s00253-013-5290-2 MINI-REVIEW Overview of thermostable DNA polymerases for classical PCR applications: from molecular and biochemical fundamentals to commercial systems Kay Terpe Received: 22 July 2013 /Revised: 20 September 2013 /Accepted: 22 September 2013 /Published online: 1 November 2013 # Springer-Verlag Berlin Heidelberg 2013 Abstract During the genomics era, the use of thermostable before becoming standard. Time and temperature of denaturing DNA polymerases increased greatly. Many were identified and can deactivate the polymerase more or less depending on the described—mainly of the genera Thermus, Thermococcus and used enzyme. Factors like DNA origin, primer and product Pyrococcus. Each polymerase has different features, resulting length as well as guanine–cytosine content should have a direct from origin and genetic modification. However, the rational influence on the choice of polymerase (Wu et al. 1991). Salt, choice of the adequate polymerase depends on the application magnesium and deoxyribonucleotide triphosphate (dNTP) con- itself. This review gives an overview of the most commonly centrations can greatly affect the PCR (Ling et al. 1991; used DNA polymerases used for PCR application: KOD, Pab Owczarzy et al. 2008). Additives like BSA (Al-Soud and (Isis™), Pfu, Pst (Deep Vent™), Pwo, Taq, Tbr, Tca, Tfi, Tfl, Rådström 2001), dimethylsulfoxide (Chester and Marshak Tfu, Tgo, Tli (Vent™), Tma (UITma™), Tne, Tth and others. 1993), formamide (Sarkar et al. 1990), betaine (Henke et al. 1997; Rees et al. 1993), ethylene glycol and 1,2-propanediol Keywords Thermostable DNA polymerase . Polymerase (Zhang et al. 2009), and others (Al-Soud and Rådström 2000; chain reaction (PCR) .
    [Show full text]
  • (12) Patent Application Publication (10) Pub. No.: US 2017/0002401 A1 TRINH Et Al
    US 2017.0002401A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2017/0002401 A1 TRINH et al. (43) Pub. Date: Jan.5, 2017 (54) COMPOSITIONS, KITS AND METHODS FOR (60) Provisional application No. 61/357,031, filed on Jun. SYNTHESIS AND/OR DETECTION OF 21, 2010. NUCLEC ACDS (71) Applicant: LIFE TECHNOLOGIES Publication Classification CORPORATION, Carlsbad, CA (US) (51) Int. Cl. (72) Inventors: Christopher TRINH, San Jose, CA CI2O I/68 (2006.01) (US); Tom XU, Castro Valley, CA (52) U.S. Cl. (US); Yating SHI, San Leandro, CA CPC ....... CI2O I/686 (2013.01); C12Y 207/07007 (US); Ferrier LE, San Jose, CA (US); Claire MARJORIBANKS, Campbell, (2013.01); C12O 1/6848 (2013.01) CA (US) (21) Appl. No.: 15/198,689 (57) ABSTRACT (22) Filed: Jun. 30, 2016 A composition comprising a thermostable DNA polymerase; Related U.S. Application Data and a PCR inhibitor blocking agent, wherein the PCR (62) Division of application No. 13/165,571, filed on Jun. inhibitor blocking agent is present in an amount effective to 21, 2011, now Pat. No. 9,410,194. enhance tolerance of an assembled PCR to a PCR inhibitor. Patent Application Publication Jan. 5, 2017. Sheet 1 of 13 US 2017/0002401 A1 37.5 Assay Pane O ACADV. A. APOE 35 v ASB7 C2 KXED3 32.5 WSF3 27.5 25 22.5 Taqian(8) Fast Advanced Taqiang Universal PCR ?iaster fix Master ?ix FG. 1 Patent Application Publication Jan. 5, 2017. Sheet 2 of 13 US 2017/0002401 A1 OOO ...i. --- 2 3 4 5 6 F 8 20 222 23 24 25 26 27 28 29 303 Cycle Number FG.
    [Show full text]
  • SUPPORTING INFORMATION Deoxynucleoside Triphosphates
    Electronic Supplementary Material (ESI) for Organic & Biomolecular Chemistry This journal is © The Royal Society of Chemistry 2013 SUPPORTING INFORMATION Deoxynucleoside triphosphates bearing histamine, carboxylic acid, and hydroxyl residues – Synthesis and biochemical characterization Marcel Hollenstein* Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland. Table of Contents: 1. General procedures 1 2. Synthesis of compounds S1-S3 3-5 3. Oligonucleotides 5 4. Additional primer extension reactions 7-9 5. Conversion of modified DNA to unmodified DNA 10-11 6. Sequencing reactions 12-15 7. References 16 1 Electronic Supplementary Material (ESI) for Organic & Biomolecular Chemistry This journal is © The Royal Society of Chemistry 2013 1. General procedures: All reactions were performed under Ar in flame-dried glassware. Anhydrous solvents for reactions were obtained by filtration through activated aluminum oxide, or by storage over 4Å activated molecular sieves. Flash chromatography was performed using silica gel (230–400 mesh) from Silicycle. Thin layer chromatography was carried out on precoated glass-backed plates of silica gel (0.25mm, UV254) from Macherey-Nagel. All chemicals and solvents used were purchased from Sigma-Aldrich, unless stated otherwise. 4-pentynoic acid methyl ester was synthesized following a literature procedure.1 5-iodo-2'-deoxyuridine was purchased from AK Scientific and 5-iodo-2'-deoxycytidine was purchased from Carbosynth. NMR spectra were recorded on a Bruker DRX-400 or a Bruker AC-300 spectrometer (400 or 300 MHz for 1H, 101 or 75.5 MHz for 13C, 121.4 MHz for 31P, and 376.5 MHz for 19F) and all spectra were referenced to the signals of the corresponding solvent.
    [Show full text]
  • UPF1 Promotes the Formation of R Loops to Stimulate DNA Double-Strand Break Repair ✉ Greg H
    ARTICLE https://doi.org/10.1038/s41467-021-24201-w OPEN UPF1 promotes the formation of R loops to stimulate DNA double-strand break repair ✉ Greg H. P. Ngo 1, Julia W. Grimstead1 & Duncan M. Baird 1 DNA-RNA hybrid structures have been detected at the vicinity of DNA double-strand breaks (DSBs) occurring within transcriptional active regions of the genome. The induction of DNA- RNA hybrids strongly affects the repair of these DSBs, but the nature of these structures and 1234567890():,; how they are formed remain poorly understood. Here we provide evidence that R loops, three-stranded structures containing DNA-RNA hybrids and the displaced single-stranded DNA (ssDNA) can form at sub-telomeric DSBs. These R loops are generated independently of DNA resection but are induced alongside two-stranded DNA-RNA hybrids that form on ssDNA generated by DNA resection. We further identified UPF1, an RNA/DNA helicase, as a crucial factor that drives the formation of these R loops and DNA-RNA hybrids to stimulate DNA resection, homologous recombination, microhomology-mediated end joining and DNA damage checkpoint activation. Our data show that R loops and DNA-RNA hybrids are actively generated at DSBs to facilitate DNA repair. 1 Division of Cancer and Genetics, School of Medicine, Cardiff University, Cardiff, UK. ✉email: [email protected] NATURE COMMUNICATIONS | (2021) 12:3849 | https://doi.org/10.1038/s41467-021-24201-w | www.nature.com/naturecommunications 1 ARTICLE NATURE COMMUNICATIONS | https://doi.org/10.1038/s41467-021-24201-w elomeres are nucleoprotein structures at the end of linear Results Tchromosomes that protect the DNA from being recognized DNA resection contributes to deletion formation at telomere as DNA double-strand breaks (DSBs).
    [Show full text]
  • INAUGURAL – DISSERTATION Zur Erlangung Der Doktorwürde Der
    INAUGURAL – DISSERTATION zur Erlangung der Doktorwürde der Naturwissenschaftlich-Mathematischen Gesamtfakultät der Ruprecht-Karls-Universität Heidelberg vorgelegt von Diplom-Biologe Michael Heide aus Esslingen am Neckar Tag der mündlichen Prüfung: ................................. Thema Die genetische Regulation der Entwicklung des kaudalen Hypothalamus Gutachter: Prof. Dr. G. Elisabeth Pollerberg PD Dr. Andrea Wizenmann Zusammenfassung Der Mammillarkörper ist ein großer Kern im kaudalen Hypothalamus, der essentiell für die Bildung von Erinnerungen ist. Patienten mit einem degenerierten Mammillarkörper können keine neuen Erinnerungen bilden (anterograde Amnesie; Wernicke-Korsakoff-Syndrom). Trotz dieser wichtigen Aufgabe sind die genetischen Faktoren, die die Entwicklung dieses Kerns regulieren, noch unbekannt. Der Transkriptionsfaktor Lhx5 ist während der Entwicklung spezifisch im Mammillarkörper eXprimiert. In dieser Arbeit analysierte ich durch in situ EXpressionsanalysen, DNA-Microarrays, qRT-PCR, ChIP-Seq und Luciferase- Assays eine Lhx5-mutante Mauslinie. Meine Ergebnisse zeigten, dass Lhx5 ein Netzwerk von Genen reguliert, die essentiell für die Spezifizierung des Mammillarkörpers sind. Als erstes zeigte ich, dass in diesen Mäusen der Mammillarkörper schon früh in der Entwicklung fehlt. Anschließend konnte ich reduzierte Proliferation und erhöhte Apoptose als Gründe für den Verlust ausschließen. Um das Schicksal der Zellen des Mammillarkörpers zu untersuchen, nutzte ich die FoXb1-Cre X ROSA26R Mauslinie, die diese Zelllinie markiert.
    [Show full text]