CTH monoclonal antibody (M03), clone S51

Catalog # : H00001491-M03 規格 : [ 100 ug ] List All

Specification Application Image

Product Mouse monoclonal antibody raised against a full length recombinant Western Blot (Tissue lysate) Description: CTH.

Immunogen: CTH (AAH15807, 1 a.a. ~ 405 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence: MQEKDASSQGFLPHFQHFATQAIHVGQDPEQWTSRAVVPPISLSTTFK QGAPGQHSGFEYSRSGNPTRNCLEKAVAALDGAKYCLAFASGLAATVTI enlarge THLLKAGDQIICMDDVYGGTNRYFRQVASEFGLKISFVDCSKIKLLEAAIT Western Blot (Cell lysate) PETKLVWIETPTNPTQKVIDIEGCAHIVHKHGDIILVVDNTFMSPYFQRPLA LGADISMYSATKYMNGRSDVVMGLVSVNCESLHNRLRFLQNSLGAVPSPI DCYLCNRGLKTLHVRMEKHFKNGMAVAQFLESNPWVEKVIYPGLPSHPQ HELVKRQCTGCTGMVTFYIKGTLQHAEIFLKNLKLFTLAESLGGFESLAE LPAIMTHASVLKNDRDVLGISDTLIRLSVGLEDEEDLLEDLDQALKAAHPP SGSHS

Host: Mouse enlarge Western Blot (Transfected Reactivity: Human, Mouse lysate)

Isotype: IgG1 Kappa

Quality Control Antibody Reactive Against Recombinant Protein. Testing:

enlarge

Western Blot (Recombinant protein)

Immunohistochemistry (Formalin/PFA-fixed paraffin- embedded sections)

Western Blot detection against Immunogen (70.07 KDa) .

In 1x PBS, pH 7.4 Storage Buffer: enlarge

Storage Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. Sandwich ELISA (Recombinant Instruction: protein)

MSDS: Download

Datasheet: Download

Publication Reference enlarge

1. Dysregulation of cystathionine γ-lyase promotes prostate cancer progression and ELISA metastasis. Wang YH, Huang JT, Chen WL, Wang RH, Kao MC, Pan YR, Chan SH, Tsai KW, Kung HJ, Lin KT, Wang LH.EMBO Rep. 2019 Oct 4;20(10):e45986. doi: 10.15252/embr.201845986. Epub 2019 Aug 29. Page 1 of 5 2021/6/19 2. Cystathionine-γ-lyase expression is associated with mitochondrial respiration during sepsis-induced acute kidney injury in swine with atherosclerosis. Merz T, Wepler M, Nußbaum B, Vogt J, Calzia E, Wang R, Szabo C, Radermacher P, McCook O.Intensive Care Med Exp. 2018 Oct 20;6(1):43. doi: 10.1186/s40635-018- 0208-z. 3. Relationship between selenoprotein P and selenocysteine lyase: Insights into selenium metabolism. Seale LA, Ha HY, Hashimoto AC, Berry M.Free Radic Biol Med. 2018 Mar 20. [Epub ahead of print] 4. The viability of primary hepatocytes is maintained under a low cysteine-glutathione redox state with a marked elevation in ophthalmic acid production. Lee J, Sil Kang E, Kobayashi S, Homma T, Sato H, Geuk Seo H, Fujii J.Exp Cell Res. 2017 Dec 1;361(1):178-191. doi: 10.1016/j.yexcr.2017.10.017. Epub 2017 Oct 26. 5. Increased ophthalmic acid production is supported by amino acid catabolism under fasting conditions in mice. Kobayashi S, Lee J, Takao T, Fujii J.Biochem Biophys Res Commun. 2017 Jul 27. [Epub ahead of print] 6. Ascorbic acid prevents acetaminophen-induced hepatotoxicity in mice by ameliorating glutathione recovery and autophagy. Kurahashi T, Lee J, Nabeshima A, Homma T, Kang ES, Saito Y, Yamada S, Nakayama T, Yamada KI, Miyata S, Fujii J.Arch Biochem Biophys. 2016 Jun 7;604:36-46. 7. Impacts of CD44 knockdown in cancer cells on tumor and host metabolic systems revealed by quantitative imaging mass spectrometry. Ohmura M, Hishiki T, Yamamoto T, Nakanishi T, Kubo A, Tsuchihashi K, Tamada M, Toue S, Kabe Y, Saya H, Suematsu M.Nitric Oxide(2014), doi:10.1016/ j.niox.2014.11.005 8. cGMP-Dependent Protein Kinase Contributes to Hydrogen Sulfide-Stimulated Vasorelaxation. Bucci M, Papapetropoulos A, Vellecco V, Zhou Z, Zaid A, Giannogonas P, Cantalupo A, Dhayade S, Karalis KP, Wang R, Feil R, Cirino G.PLoS One. 2012;7(12):e53319. doi: 10.1371/journal.pone.0053319. Epub 2012 Dec 28. 9. Hydrogen sulfide impairs glucose utilization and increases gluconeogenesis in hepatocytes. Zhang L, Yang G, Untereiner A, Ju Y, Wu L, Wang R.Endocrinology. 2013 Jan;154(1):114-26. doi: 10.1210/en.2012-1658. Epub 2012 Nov 26. 10.An investigation of the mechanisms of hydrogen sulfide-induced vasorelaxation in rat middle cerebral arteries. Streeter E, Hart J, Badoer E.Naunyn Schmiedebergs Arch Pharmacol. 2012 Jul 17. 11.Characterization of Hydrogen Sulfide and Its Synthases, Cystathionine β-Synthase and Cystathionine γ-Lyase, in Human Prostatic Tissue and Cells. Guo H, Gai JW, Wang Y, Jin HF, Du JB, Jin J.Urology. 2012 Feb;79(2):483.e1-5. 12.Glucocorticoids suppress cystathionine gamma-lyase expression and H(2)S production in lipopolysaccharide-treated macrophages. Zhu XY, Liu SJ, Liu YJ, Wang S, Ni X.Cell Mol Life Sci. 2010 Jan 10. [Epub ahead of print] 13.H2S contributes to the hepatic arterial buffer response and mediates vasorelaxation of the hepatic artery via activation of KATP channels. Siebert N, Cantre D, Eipel C, Vollmar B.Am J Physiol Gastrointest Liver Physiol. 2008 Dec;295(6):G1266-73. Epub 2008 Oct 30.

Applications

Western Blot (Tissue lysate)

Page 2 of 5 2021/6/19 CTH monoclonal antibody (M03), clone S51. Western Blot analysis of CTH expression in mouse kidney. Protocol Download

Western Blot (Cell lysate)

CTH monoclonal antibody (M03), clone S51 Western Blot analysis of CTH expression in K- 562 ( Cat # L009V1 ). Protocol Download

Western Blot (Transfected lysate)

Western Blot analysis of CTH expression in transfected 293T cell line by CTH monoclonal antibody (M03), clone S51.

Lane 1: CTH transfected lysate(45 KDa). Lane 2: Non-transfected lysate. Protocol Download

Western Blot (Recombinant protein) Protocol Download

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Page 3 of 5 2021/6/19 enlarge this image Immunoperoxidase of monoclonal antibody to CTH on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml] Protocol Download

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged CTH is approximately 1ng/ml as a capture antibody. Protocol Download

ELISA

Gene Information

Entrez GeneID: 1491

GeneBank BC015807 Accession#:

Protein AAH15807 Accession#:

Gene Name: CTH

Gene Alias: MGC9471

Gene cystathionase (cystathionine gamma-lyase) Description:

Omim ID: 219500, 607657

Gene Ontology: Hyperlink

Gene Summary: This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq

Other OTTHUMP00000010945,OTTHUMP00000010946,cystathionase,cystein Designations: e desulfhydrase,gamma-cystathionase,homoserine deaminase,homoserine dehydratase

Gene Pathway Page 4 of 5 2021/6/19 Cysteine and methionine metabolism Glycine, serine and threonine metabolism Metabolic pathways Nitrogen metabolism Selenoamino acid metabolism

Related Disease Atherosclerosis Atherosclerosis Calcinosis Cardiovascular Diseases Carotid Stenosis Cleft Lip Cleft Palate Coronary Artery Disease Diabetes Mellitus, Type 2 Edema Genetic Predisposition to Disease Hypertension Infertility, Female Lung Neoplasms Pulmonary Disease, Chronic Obstructive Spinal Dysraphism Urinary Bladder Neoplasms Werner syndrome

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