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... [22] B1 The use of Hyperbaric Oxygen in Necrotising Soft Tissue Infections: A Historical Perspective with an Update anno 2008 D. J. Bakker, MD, PhD. Amsterdam, the Netherlands

Introduction: Necrotizing soft tissue infections caused by aerobic, anaerobic and mixed bacterial floras are a still increasing problem in surgical and medical practice. They occur with increasing frequency and seriousness, especially in immune-compromised patients. The clinical picture can vary considerably from patient to patient. Treatment is difficult, often irrational and very often "one step behind the facts," because early recognition is difficult and etiology , bacteriology, and the clinical course are aetiology sometimes not well understood or expected to evolve in a different and more favourable way. Hyperbaric Oxygen Odstraněno: treatment (HBOT) is a highly controversial issue in the literature. Considerable morbidity occurs in these diseases and the mortality can be very high, from 20% up to 70 or 80%.

History: For a proper understanding it is necessary to give a short review of the history of these infections including the bacteriology and the various forms of treatment that have been advocated and practised.

In this review we will pay attention to the works of Hippocrates from the 5 th century BC, Baurienne (1764), Hebler (1848) and Jean Alfred Fournier (1883), a French venereologist, Also the work by the Confederate Army Surgeon Joseph Jones will be mentioned who described a variant of this disease sthese in 1869 and 1870 during the Civil War in the United States. Odstraněno: diseases

Very relevant are also the works of Meleney and Cullen (1924) en Brewer and Meleney (1926). Meleney reported about his work in the Imperial Hospital In Peking (now Beijing) in China.

Extensive reviews on necrotizing soft tissue infections including Fournier’s gangrene have been published and can be found in the literature (Bakker 2002, Bakker 2008). The immense confusion in the nomenclature and the classification of these diseases will be explained.

The role of Hyperbaric Oxygen in the treatment of these disease started in 1959 when Boerema and Brummelkamp treated a patient with Gas Gangrene in the Hyperbaric Chamber (HBOT). The first Congress on HBOT was held in Amsterdam in 1963, now 45 years ago.

Material and Methods: We will restrict ourselves in this presentation to Clostridial Myonecrosis or Gas Gangrene and Necrotising Fasciitis or Fourniers Gangrene. a. Necrotising Fasciitis (NF) is a progressive, generally rapid spreading, inflammatory process located in the deep fascia with secondary necrosis of subcutaneous tissues and skin. The infection tends to spread very rapidly along the deep fascial planes. There is confusion in the literature about the microorganisms that cause NF. Haemolytic Streptococci play a major role in the onset of the disease. Later on in the disease one can find many more microorganisms, from which their role in the disease is not clear. A rapid diagnosis is essential for treatment and prognosis. Treatment is a combination of proper, early and extensive surgery, antibiotics and adjunctive HBOT. The grade of evidence for using HBOT in the treatment will be shown from the literature data and our own experiences. b. Clostridial Myonecrosis or Gas Gangrene is an acute, rapidly progressive, non-pyogenic, gasforming and necrotising infection of muscles, subcutaneous tissues and skin. The infection is caused by anaerobic spore-forming bacteria of the genus Clostridium. Although the prognosis of the disease is improved by modern antibiotics and intensive care treatment, the mortality and morbidity is still high, especially without HBOT. A rapid diagnosis has to be made, immediately followed by HBOT, not losing any time by performing initial surgery. The patients are too ill for surgery. Treatment consists of immediate HBOT and antibiotics, followed by, if necessary, surgical measures secondarily. This treatment has proven its use in several thousands of patients treated in this way, with constant good results over the years. Here also an update will be give about the best treatment modality anno 2008 and the evidence based reasons for that from the literature and from our own results. c. Evidence based medicine in HBOT (EBHM) is the combination of old and trusted clinical skills completed by and if necessary also corrected by the critically appraised best available scientific research results and the evaluation of care. The idea is to move beyond clinical experience and physiological principles to rigorous evaluations of the consequences of clinical actions. These evaluations are in too many indications in hyperbaric medicine, non-existent. When looking for the evidence of HBOT in infectious diseases several steps have to be taken. First of all the clinical problem has to be appropriately formulated and the correct diagnosis has to be made. Therefore clinical expertise is required. Nothing can substitute for clinical expertise in determining the specific considerations relevant to the individual patient. Clinical skills are necessary for EBHM plus an understanding of how the patients values affect the balance between advantages and disadvantages of the available treatment options, and the ability to appropriately involve the patient in the decision. Every decision taken on the basis of education and experience should, however, also be based on results of trials and evaluations. In this way we can define HBOT as a primary mode of treatment or as an adjunctive mode. We must be careful not to move the focus of clinical practice away from the care of individuals to the care of populations, lacking than the ability to apply this knowledge back to our individual patient. The Randomised Controlled Clinical Trial (RCT) is nowadays the gold standard in EBHM, but we must not forget that there is more evidence than that, that has to be taken into account when judging HBOT.

Conclusion: After a short description of soft tissue infections in the history of medicine, the state of the art in diagnosis and treatment of some special forms of soft tissue infections, eg. necrotising fasciitis and clostridial myonecrosis is shown.

Especially the evidence based character of treatment with Hyperbaric oxygen based on own experiences and reports from the literature is described.

B2 Role of Tissue Oximetry in Assessment of Problem Wounds Paul J. Sheffield, PhD; International ATMO, Inc, Nix Wound Healing Center, San Antonio, Texas, USA

Transcutaneous oximetry (PtcO2) is commonly used to determine treatment options for problem wounds: surgical remedy, adjunctive hyperbaric oxygen (HBO2), or standard wound care. HBO2 wound healing candidates are selected by assessing whether the wound area is hypoxic and if it will respond to respired oxygen. There are five PtcO2 tests commonly used to evaluate wound patients.

1. Evaluate whether wound healing is complicated by severe hypoxia. Test 1: Measure a PtcO 2 baseline air value at 1 atm abs. Hypoxia exists if PtcO2 is less than 40 mmHg. The lower the PtcO2 value, the more severe is the hypoxia.

2. Evaluate whether wound healing is complicated by peripheral arterial occlusive disease. Test 2: Elevate the leg (30˚). Disease is present if PtcO2 remains diminished more than 10 mmHg while leg is elevated.

3. Evaluate whether the wound area responds to respired O2. Test 3: Conduct an oxygen challenge at 1 atm abs. Values on respired oxygen should reach at least 35 mmHg and should rise at least 50% above air PtcO2 values.

4. Evaluate whether the wound area responds to respired HBO2. Test 4 (Alternate to Test 3): Conduct an oxygen challenge at 2-2.5 atm abs. Values on HBO2 should be at least 100 mmHg (preferably 200 mmHg).

5. Evaluate whether the wound is to the point where it will heal without further HBO2 treatment. Test 5: Repeat PtcO2 baseline air value at 1 atm abs in 3-4 wk intervals. Normalized baseline values indicate that healing process is in place.

PL 1 The Indication of Hyperbaric Oxygen Therapy and for Orthopaedics in Japan Mahito Kawashima, Takashi Yamaguchi Kawashima Orthopaedic Hospital, Japan

In Japan, two types of standards of indication of Hyperbaric Oxygen Therapy (HBO) are set. One is established by Japanese Society of Hyperbaric and Undersea Medicine (JSHUM) and another is by the Ministry of Health, Labor and Welfare for public medical insurance. Most cases are treated with the standard of the Ministry of Health, Labor and Welfare, because of the medical serv. system for the whole nation in Japan. This standard, the disease is classified into two types, emergency/non-emergency. The fee for treatment is different between emergency and non-emergency, multiplace chamber and monoplace chambers. In the case of multiplace chamber, the fee for emergency is \60,000 (US$545) per usage time of HBO. In the case of monoplace chamber, the fee for emergency is \50,000 ( US$454) per usage of HBO. The fee for non-emergency is \2,000 (US$18) par usage of HBO, in the case of non-emergency the fee is no different, multiplace or monoplace chambers.

The standard of indication of HBO by the Ministry of Health, Labor and Welfare is as following.

Emergency: Acute carbon monoxide poisoning, Gas poisoning, Air gas embolism, Decompression illness, Acute peripheral vessel trouble, Thermal burns, Frostbite, Crush injury, Peripheral vessel trouble by trauma, Shock, Acute myocardial infarction, Cerebral embolism, Severe head injury, Disturbance of consciousness or brain edema after craniotomy, Hypoxic brain dysfunction, Intestinal atresia, occlusion of retinal artery, Sudden deafness, Acute spinal cord injury.

Non emergency: Malignant tumor with carcinostatic or radiation therapy, Refractory ulcer, Skin graft, SMON, Cerebrovascular disorder, Severe head injury, Motor paralysis after craniotomy, sequelae of carbon monoxide poisoning, Spinal nerve disorder, Osteomyelitis, Radiation necrosis.

According to the research on March 2008, 51 multiplace chambers and 805 monoplace chambers have been working in 644 facilities in Japan.

Kawashima Orthopaedic Hospital specializes in orthopaedic surgery, has 93 beds for admission and two multiplace hyperbaric chambers. The one has worked since 1984 and the other has worked since 1989. Kawashima Clinic is juxtaposition clinic of Kawashima Orthopaedic Hospital. A multiplace hyperbaric chamber has been there since April 2005.

The main indications of HBO in orthopaedic surgery are Gas gangrene, Acute spinal cord injury, Crush injury, Osteomyelitis and Refractory ulcer. HBO is expected for these disorders as inhibition and prevention for infection, promotion of recover of damaged cells, promotion of angiogenesis and improvement of edema. The total number of cases is 7,324 cases from 1981 to 2007 in Kawashima Orthopaedic Hospital. In these, Main orthopaedic disorders are 630 osteomyelitis, 183 crush injury, 76 spiral cord injury, 41 gas gangrene and 375 ulcer/necrosis.

PL2 The Use of “Deep Stops” In Preventing Precordial Detectable Doppler Bubbles in Recreational Scuba Divers Frans J. Cronjé, MBChB, MSc 1, Alessandro Marroni, MD 2, Peter Bennett, DSc, PhD 3 1. DAN Southern Africa; 2. DAN Europe; 3. International DAN

Decompression illness (DCI) affects some 1500 divers every year. Although DCI is relatively rare, two thirds of these divers develop neurological manifestations.

The empirical use of additional deep safety stops by recreational divers has become popular in recent years. However, these arbitrary procedures have not been evaluated for safety and effectiveness.

In order to establish appropriate and practical recommendations relevant to typical recreational divers, a series of studies were designed to evaluate the effect of adding deep stops of varying durations (1 to 10 minutes) at half-the-depth of the dive – Half Depth Deep Stops (HDDS) – with or without shallow stops at 5 meters (1 to 10 minutes), in the typical recreational diving range of 18 to 40 MSW.

The introduction of a HDDS generally reduced post-dive precordial Doppler detectable venous gas emboli (PPDDVGE), with an overall decrease of High Bubble Grades compared to the same dives without HDDS.

The data suggest that the inclusion of a HDDS on dives between 25 and 30 MSW, with Bottom Times of 25 minutes or less (i.e., the typical dive profiles performed by recreational divers) reduces decompression stress as measured by PPDDVGE.

The value of HDDS in reducing PPDDVGE was not as evident for shallower (18 – 21 MSW) and deeper dives (40 MSW), when brought to the limit of the respective No-D bottom time according to USN Dive Tables, and showed conflicting results.

Further investigation is now being planned to unravel the apparent ambiguity of HDDS at these depths.

PL 3 An Overview of Diving and Diving Medicine in China Tao Hengyi, Sun Xuejun, Liu Wenwu, Sun Qiang, Mao Ding Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China

This article presents a brief history of diving and diving medicine in China, especially the developmental track of diving and diving medicine, as well as symbolic achievements in this field since the founding of New China. It also provides an overview of research on submarine escape or rescue in the Chinese Navy.

1. A brief history of diving and diving medicine in China Diving is an important means of human fighting against and adapting to Nature. But there is no textual research about when and where diving was started and who pioneered it. According to Shang Shu (the Book of History) published in the 21 st century BC in China, there were already people who used pearls and beads from seabed as tributes to Yu Empire in ancient China, indicating that the Chinese people started their diving activities as early as in the 21 st century BC. Nevertheless, the primitive purpose of human diving was for survival rather than for material contributions. During nearly 50 years from 1900 when China began using modern diving apparatus (Hookah diving apparatus and manual pumps) to the eve the founding of new China, there were only a few diving units with fewer than 100 divers and shabby equipment, much less diving safety operation regulations, and there were few people who had professional knowledge of diving medicine, let alone diving medical supervision systems. The maximum diving depth was about 20 meters. Diving accidents happened frequently, and the incidence of diving disease was high. Once divers were diseased, nobody helped them. Therefore, many divers were handicapped by lifetime diving illnesses, and the case-fatality rate remained at a high level.

After the founding of new China, under the attendance and care of the Chinese Communist Party and the people’s government the diving cause in New China has a new lease of life and develops flourishingly. Diving facilities are being built up in succession. Right after the founding of the Chinese Navy, arescue-salvation force was set up, which has been expanded and reinforced increasingly. The Ministry of Communications established marine rescue & salvage bureaus in Shanghai, Guangzhou and Yantai. The Ministry of Petroleum and the Ministry of Railways also established specific diving units. The number of professional divers is on the increase and their diving skills are becoming more sophisticated. Diving technology has now been widely used in economic constructions (such as tapping and exploitation of seabed resources), military activities (such as submarine rescue and underwater reconnaissance), scientific research and sports. Diving medicine has been developing out of nothing and expanding increasingly. In 1954 the Institute of Naval Medicine was inaugurated in China, which includes a research department specialized in diving medicine. In 1960, the Faculty of Naval Medicine was extended in Shanghai Second Military Medical University, which is the only institution of higher learning that can train diving physicians in China. Today diving medicine in China is essentially appropriate to the development of diving technology and the demand of divers’ safety and health. To facilitate academic exchange in diving and diving medicine, in 1980 Shanghai took the lead and established the first Hyperbaric Medicine Society in China. In 1982 the Chinese Navigation Society also established the Diving Rescue & Salvage Committee. As you know, Chinese Society on Hyperbaric Medicine was established in 1992.

Before the Cultural Revolution, China carried on a close-door policy and did not know about the world, and the world knew even less about China. Before 1987 when Professor LI Wen-ren and NI Guo-tang, two senior masters in diving and hyperbaric medicine in China, first attended the 9 th International Congress on Hyperbaric Medicine, not a few international scholars in this field even had no idea about whether China had diving operation deeper than 30 meters. So, when Professor Ni presented his MSc thesis at the meeting, a delegate even raised such a question, “Is there diving operation deeper than 30 meters in China?” It is DENG Xiao-ping’s open-to-the-outside-world policy that enables the world to know about China and enables China to know about the world. Today, I’d like to take this opportunity to let the world know more about China, especially about China’s diving and diving medicine.

2. The developmental track of diving and diving medicine in New China The first article about diving decompression sickness (DCS) was officially published in 1955. In 1960 China began using hyperbaric equipment for clinical hyperbaric oxygen treatment. The research and development of the surface decompression table and the underwater stage decompression table of air diving were started in 1961 and ended with success in 1965. They were put to practical use by the Chinese Navy in 1977. Civil diving operations in China also follow these tables. The use of the Navy decompression tables plays an extremely important role in reducing the incidence of DCS and in guaranteeing divers’ safety and health.160-msw deep heliox diving training was first started in 1962 in China. During the construction of Nanjing Changjiang Bridge in 1964, a total of 544 time-person air diving operations were undertaken to a depth of 66 meters, where the technique of surface decompression using oxygen was used without causing a single case of DCS, reaching the world advanced level. The first practical use of deep heliox diving was in 1965 in inspecting the quality of bridge piers, reaching the depth of 83 meters. In 1969, the scuba diving equipment was developed successfully in China. The first experiment of 200-msw heliox simulated diving was undertaken in 1970. In 1973 two divers used air diving into a mud cave equivalent to 92 msw to salvage a lost large drill. Although the attempt failed because of nitrogen narcosis, this was the greatest depth that air diving has been reached in the world. In 1975 deep heliox diving reached 156 mfw in China. In 1976 the first experiment of human simulated air saturation diving was undertaken, the saturation depth being 20 msw. In 1977 the first experiment of human simulated nitrox saturation diving was undertaken, reaching the saturation depth of 20-36.5 msw. During the experiment, air excursion diving successfully reached 50-70 msw in all 181 time-persons. In 1977 a diver, Mr Long, air dived to 64 meters and worked at the bottom for 25 min. But unfortunately, he was out of control and blew up to surface suddenly. As soon as he reached surface, he was paralyzed and comatose. To save this severe DCS diver, Mr. MU Ze-ling, a military diving physician accompanied the patient to the hyperbaric chamber, where the pressure was raised to 11 ATA using compressed air to meet the need of the patient’s condition. They stayed at the maximum pressure for 60 min, and the total duration of decompression was 85 h and 55 min. The patient was saved successfully. This is the maximum air pressure to which humans have been exposed. This practice reverses the previous inference that “human exposed to compressed air ≧ 11 ATA is bound to cause unconsciousness and narcotic coma”. Generally speaking, when the chamber pressure is ≧6-7 ATA, the mixture of heliox should be inhaled to prevent nitrogen narcosis. But as most hyperbaric chambers in China did not have the condition of inhaling heliox at that time, inhalation of 11 ATA compressed air was the last straw to save the patient’s life. This is also a full reflection of how dedicated the Chinese medical workers are in rescuing the wounded and saving the dying. Mr. MU is not only a military diving physician but also a teacher of diving medicine in the Department of Diving Medicne, Faculty of Naval Medicne, Second Military Medical University, Shanghai China. Owing to his selflessness and dedication, Mr. MU was honored as “Model Teacher” in 1978 by the Central Military Committee. In 1979 two experiments of simulated nitrox saturation diving were undertaken in China: the saturation depth of the first experiment was 36.5 msw for 26 days, during which air excursion reached 60-75 msw in 76 time-persons, and the saturation depth of the second experiment was 50 msw for 12 days. Both experiments were successful, taking the lead in the world both in nitrox saturation depth and duration of nitrox saturation exposure. Up to now, no nitrox saturation diving experiment has been reported to exceed the record of 50 msw and 12-day stay. In the same year, an on-the-spot nitrox saturation diving operation was performed, reaching 36.5 msw and staying at saturation depth for 6 days, during which air excursion reached 64 msw in 14 time-persons. Marine in situ practices in China have demonstrated that the results of these simulated nitrox saturation diving experiments are convincing, reliable and practicable. Still, in 1979 the first experiment of simulated helioxsaturation diving was undertaken, reaching the saturation depth of 120 msw and 2-day stay at saturation depth, during which the excursion depth reached 165 msw in 3 time-persons. During 1977-1980, a famous salvage project was completed successfully in salvaging a Japanese sunken ship called “AWAMARU”in a depth of 48-69 msw. The operation used air diving throughout, involving 13604 time-persons. This has been the largest and longest salvage project in China, in which only 80 cases of DCS occurred with an overall incidence of 0.59%. In 1980 three Chinese Navy divers succeeded in locking out from a submarine at a depth of 205 msw during their study in France Navy. In 1980 the first treatment table for air diving DCS was developed in China and has been proved to be therapeutically good. In 1981 an experiment of simulated heliox saturation diving was undertaken, during which three divers stayed at a depth of 302 meters for 43 h, and returned to surface safely and successfully after 12-day decompression. In 1982 the first deep submergence rescue vehicle (DSRV) designed and manufactured by China with self-dominated intellectual property rights cruised its first voyage. In 1989 an experiment of simulated heliox saturation diving was undertaken by the Chinese Navy, in which four divers stayed at the depth of 350 msw for 3 days (72 h and 10 min), and excursion diving reached 370-374 msw in 8 time-persons. After 14-day decompression all four divers returned to surface safely. That was the deepest record of saturation diving in Asia at that time. In 1997 the Chinese Navy conducted a heliox saturation diving training on the sea, the storage depth of which was 120 mswn for 48 h, during which the excursion dive reached 149 msw in 8 time-persons. In 2001 the Chinese Navy conducted two heliox saturation diving trainings. The saturation depth of the first training was 140 msw for 48 h, during which the excursion dive reached 166 msw in 8 time-persons, and that of the second training was 160 msw for 48 h, during which the excursion dive reached 182 msw in 4 time-persons. It was in 2006 that saturation diving was first used for commercial purposes. During October to December 2006, Shanghai Salvage Bureau undertook a commercial heliox saturation diving operation for replacing oil pipes at a depth of 96 msw in South China Sea; the maximum depth of excursion diving was 105 msw. During August to November 2007, Shanghai Salvage Bureau undertook a 115-msw commercial heliox saturation diving operation in the South China Sea Oil Field for clearing, measuring and installing oil pipes; the maximum depth of excursion diving was 124 msw. In April 2008, the same company undertook a 135-msw commercial heliox saturation diving operation for the same tasks as the previous ones; the maximum depth of excursion diving was 153 msw. In these commercial operations, PPO 2 at the saturation depth was maintained at 0.4-0.5 ATA; PPCO 2 was maintained at <0.0055 ATA; chamber temperature was 28.1-30.5 ℃; and relative humidity was 56.6-81.1%. During the process of decompression, PPO 2 in the chamber was raised to 0.6 ATA. The whole compression was divided into two phases: at first, the pressure was raised to 5 msw using 20/80 He-O2, and the second, the pressure was continued to compress to saturation depth using 2/98 He-O2 at a rate of 1-2 m/min.The saturation decompression rate was controlled at 40-60 min/m. During these commercial saturation diving operations, except two cases of skin infection, no other medical problems occurred because of excellent diving medical supervision.

3. Submarine Escape in China Submarines are among the most important combat weapons for the navy and specially favored by the navy because of their good stealth and strong assaulting ability. But like aeroplanes that may be threatened by air crashes and automobiles that are likely to be involved in road accidents, submarines have their own problems. In exercises or combat operations, submarines may run on collisions and rocks, or have mechanical problems, or lose the floating ability when they are attacked by the enemy. It is roughly estimated that from 1900 to 2001 a total of 434 submarine accidents happened during the peace-time training exercises in American, Russian, British and French armed forces, excluding those that are not reported because of confidential reasons. These accidents involved 176 sunken submarines at least killing 2900 crew members. The most astonishing accident was Russian Kursk submarine due to torpedo explosion on August 12, 2000. The submarine sank to the 108 msw sea floor killing 118 soldiers and officers on the ship. To preserve living strength, timely and effective rescue measures must be taken to help the survivors escape from the wrecks. Therefore, how to help crews escape from the wrecks has long been the focus of attention for navies of all countries. The earliest study on submarine escape techniques and methods in the world was in 1851. As the submarine force in China was established at a relatively late time, the study on submarine escape techniques and methods was started in 1960, but has been progressing quickly. In 1970, type 2-8 submarine escape equipment and the decompression table for submarine escape were developed successfully, by which individual escape in the depth of 120 msw can be effected. Individual escape follewed by mass escape would provide escape from a 200 msw depth. Subsequently, studies on fast ascent and its medical supervision were started. During 1976-1979, simulated 10-310 msw animal experiments were conducted, and in 1980 experiments were performed on humans. In 1983, the human simulated 100 msw fast ascent experiment was accomplished successfully. In 1987, the submarine escape training from the depth of 52 msw through the torpedo tube was accomplished. This is an important creation of the Chinese Navy, because fast ascent from submarine in other countries is implemented through a specific one man escape chamber (OMEC) on the submarine. It has been confirmed by practice that submarine escape from torpedo tube is feasible. In 2002, the study of simulated 153 msw fast ascent was successfully accomplished and created the deepest record of fast ascent for submariners in Asia, thus upgrading China’s research in this field to the world advanced level.

PL4 DCS Incidence of Commercial Dive Works In Japan and Evaluation of the Profile on Multi-Level Diving By Integration of Nitrogen Accumulation in Each Body Tissue

Yoshihiro Mano 1, Masaharu Shibayama 1, Masahisa Komiya 1, Shin-ichiro Togawa 1, Nobuo Yamami 1, Kazuyoshi Yagishita 1, and Mahito Kawashima 2 1. Hyperbaric Center, Tokyo Medical & Dental University Hospital, Japan 2. Kawashima Orthopedics Hospital, Japan

Commercial dive work has been strictly controled in Japan, so there were few accident cases recently. According to our data which we have consulted since 1995, the DCS incidence was 0.095 % ( 31 bends per 32,639 dive ) in air dive within 60 m depth and 0.099 % ( 3 bends per 3,038 dive ) in heliox dive works from 30 to 90 m depth. In saturation diving work by heliox within 200 m depth, it has been 0.38 % ( 2 bends in 529 dive ) since 1983. We have used different dive methods like as scuba, air line, bell and saturation. And also deffernt decompression tables like as the Japanese or U.S. Navy standard, surface, and/or our original tables included saturation, however it depends on the dive conditions. This result has been evaluated as considerably better than other commercial dive companies in Japan.

However in recrational or fishermen divers, there have been recognized near 40 to 50 diving accidences and 20 to 30 divers have died in each year. And some of them have died by the cause of DCS or AGE. Their diving profiles and the continued decompression methods are obviously risky or self-seeking. So we have developed a new analyzer system to evaluate dive profile through integration of the nitrogen gas accumulation in each body tissue from 5 to 240 min half saturation time. We can calculate the accumulated tisue gas tension during diving activity in each 5 second and can judge whether the diving profile has the risk of DCS or not at the surface by means of compareing it with Workman’s M Value.

Japanese standard regulation has now been looking again and it will bocome to better and safer working field in near future.

PL5 Traumatic Spinal Cord Injuries – is There a Role for HBO?

Jochen Freier 1, MD; Armin Kemmer 2, MD 1. Consultant für Diving and Hyperbaric Medicine (EDTC), Anaesthesiology and Intensive Care, Vice President of the German Society für Hyperbaric Medicine (GTUEM), Tagesklinik Hofheim, Germany 2. Consultant für Diving and Hyperbaric Medicine (EDTC), Anaesthesiology and Intensive Care, Habach, Germany

Spinal cord injuries are still among the conditions, which are extremely difficult to treat.

Damage of the nerve strands embedded in the lumen of the spinal cord often results in permanent paraplegia. In addition to direct damage to the spinal cord through ruptures and crushes, the injured tissue suffers from a lack of oxygen in the aftermath of an accident. This causes the cascading release of various tissue hormones like cytokines and prostaglandins, which lead to the swelling of tissue, local inflammation and, ultimately, cell death. These processes cause additional damage to the site of the injury and often result in irreversible damage. The basic principle of surgical therapy for thoracic and lumbar spinal cord injuries relies on decompression, reposition and the subsequent stabilization of the unstable segment. In addition, early administration of methylprednisolone, ideally already at the site of the accident, is recommended.

These treatments ultimately aim at improving the tissue’s blood supply and restoring aerobic cell metabolism as quickly as possible. This is the rationale for hyperbaric oxygen therapy. Analogous to the treatment of crush trauma, HBO results in a rapid reoxygenation of tissue, which helps to maintain viability and can thus reduce the loss of function.

CNS injuries will cause local changes within a few minutes. These changes not only occur in neurons but also in glia cells. In contrast to CNS neurons, peripheral neurons are able to cover a greater distance downstream of the site of the injury, and there, to form synapses to transfer excitation signals to their target cells. By contrast, CNS neurons stop the process of regeneration immediately once they have reached the site of the injury. The rapidly forming gliatic scar prevents continuing axonal growth (Müller et al. 2005, Stichel et al. 1998). The inhibition of cellular collagen synthesis leads to the permeability of the lesion scar for regrowing axons (Müller et al. 2005).

In a study by Murakami (2001), the neurological functions of rabbits with spinal cord ischemia improved when HBO therapy was initiated after 30 minutes. However, no improvement was seen when therapy was started after 6 h. Huang (2003) also observed an improved neurological recovery from spinal trauma in rats when HBO therapy was given.

Likewise, Gamache (1980) could demonstrate a more rapid recovery of motor scores in spinal cord trauma after early HBO therapy. In the Trauma Center Murnau, 35 out of 40 patients with acute traumatic paraplegia showed a significant improvement under hyperbaric oxygen therapy. Asamoto et al (2000) could also achieve a significant improvement under hyperbaric conditions. Likewise, Ishihara et al. (1997) observed a positive effect for the application of hyperbaric oxygen therapy.

In the Trauma Center Murnau a randomised study design was developed, which is presented herein.

The positive effect could be confirmed in several trials. If this effect is solely due to the swell-reducing effect and the improved oxygenation has not been adequately studied. The application of hyperbaric oxygenation after ordinary injuries has shown that its application results in reduced scar formation, i.e. collagen production, which could be another reason for the positive effect of hyperbaric oxygen therapy in spinal cord injuries.

Further studies on the possible changes in collagen production under hyperbaric conditions would be desirable.

PL6 Hyperbaric Oxygen Therapy in Acute Traumatic Peripheral Ischemic Diseases Wang Gang M.D. Ph.D. Emergency Dept & Hyperbaric Oxygen Dept. First Affiliated Hospital, China Medical University, Shenyang, China

HBO can be used as adjunctive therapy for trauma patients with crush injury, compartment syndrome, and other acute traumatic peripheral ischemia (ATPI). The rationale for HBOT is the need to counter the effects of tissue hypoxia arising as a direct consequence of vascular injury and as a secondary consequence of trauma and infection.

Physiological and Pharmacological Basis of HBO Therapy for ATPI 1.Hyperoxygenation. 2.Increasing in oxygen diffusion distances. 3.Promoting cellular proliferation, accelerating collagen deposition. 4.Stimulating capillary budding and arborization. 5. Modulating nitric oxide production. 6.Possibly preventing iNOS up-regulation. 7.Enhancing oxygen radical scavengers, reducing neutrophil endothelial adherence, thereby decreasing I/R injury. 8.Vasoconstriction and reduction of edema. 9.Maintenance of cellular function. 10. Accelerating microbial oxidative killing. 11.Improving select antibiotic exchange across menbrances. 12. Interfering with bacterial propagation.

Crush injury/ Skeletal muscle-compartment syndromes Crush injury and skeletal muscle-compartment syndrome (SMCS) are two related conditions that arise as a consequence of trauma. Common features include ischemia and hypoxia at the injury site, a gradient of injury, and the potential for self perpetuation of the injury. 1. Etiology 2. Pathophysiology 3. Ischemia 4. Edema 5. Gradient of injury The principles of treatment for crush injury Surgical and orthopedic interventions for crush injury are based on the type of injury and should be independent of the decision whether or not to use HBO. That is, HBO should not be used as an excuse to delay surgery or not do surgery. However, when the decision to use HBO is made, it should be started as soon after the injury as possible. If there are delays in starting surgery, And HBO is available, it should be given while awaiting the availability of the operating room.

Scheme of Adjunctive HBO Therapy There is no single ideal protocol for using HBO in crush injury. Treatment schedules are largely a function of clinical judgment and experience. The maintenance of tissue oxygenation is critical in the immediate post injury period.

The management of Skeletal-Muscle Compartment Syndrome The diagnosis of compartment syndrome is based on the mnemonic of “five Ps. The monitoring of the pressure in compartment is important for evaluation of the condition and progression of injured tissue.

The principle of therapy for SMCS Management of the established SMCS is fasciotomy. This decompresses the muscle and immediately lowers the compartment pressure, thereby restoring perfusion to the contents of the compartment.

Convincing laboratory studies and clinical researches show statistically significant reduction, in loss of muscle function, metabolites associated with muscle injury, edema and muscle necrosis with HBO.

Scheme of Adjunctive HBO Therapy

HBOT in Flap Reconstruction and Skin Grafts Normal skin grafts and flaps with adequate blood supply do not requir HBO. HBO therapy is extremely useful where the skin grafts or flaps suffer from compromised microcirculation or hypoxia. The benefits of HBO on flaps arise from a systemic elevation in oxygen tension rather than a local effect. In addition, HBO therapy prevents neutrophil adherence and subsequent vasoconstriction following ischemia.

Compromised Flaps Too frequently, a compromised flap is allowed to progress over the days following surgery until visible signs of necrosis obviate the use of HBO; delayed treatment with HBO cannot revive dead tissue. The resulting disappointment, as well as the associated patient dissatisfaction, can be avoided by rapid diagnosis of the flap problem and early involvement of the hyperbaric physician.

Diagnosis of Flap Compromise Compromised tissues are usually hypoxic with oxygen tension of less than 30mmHg. With flaps, the etiology of the flap ischemia and compromise may result from technical causes including improper flap design, closure with tension, pedicle or tissue damage, hematoma, or prolonged operative ischemia. Non-technical causes include arterial vasospasm, flap edema, post-operative infection, and patient deterioration. Special flap problems include low arterial inflow, total arterial occlusion, partial venous congestion, and total venous occlusion.

HBO Treatment for Compromised flaps The use of HBO therapy is appropriate only when: 1. The flap problem has been defined 2. There is documented perfusion of flap 3. Appropriate surgical salvage measures have been considered first 4. HBO therapy can be performed in an expedient manner

Scheme of HBO therapy for compromised flaps HBO therapy for compromised flaps should be based on the type of flap, classification of ischemia, and effects of treatment of HBO, which decided HBO therapy regimen.

HBO Therapy for I/R injury Initially, the focus in acute ischemia caused by trauma should be restoration of blood supply. Replantation should be completed as soon as possible with repair of disrupted arteries and fasciotomies if necessary. Both clinical experience and the previously summarized research experience advocate for HBO treatment in I/R injury and results are positive and often dramatic in our experience. If muscle ischemia time is greater than four to six hours there is a significant risk of severe I/R injury, muscle necrosis, and loss of the affected extremity. Skin is more resistant to I/R injury but it is likely to occur after eight hours of ischemia as in the case of a digital amputation. We, therefore, recommend HBO therapy for all patients with muscle ischemia time greater than four hours and skin ischemia time greater than eight hours.

A retrospective controlled review of compromised free flaps and replanted extremities with greater than six hours of primary and any secondary ischemia showed 100% salvage rate when HBO therapy was initiated within 24 hours of injury and 0% salvage rate when HBO therapy was initiated greater than 72 hours after the injury. The major effects of I/R injury are felt to occur within the first 4-7 hours of reperfusion. Since some irreversible tissue damage occurs after this time it is important to take patients to receive HBO therapy immediately postoperatively, even if they are still intubated.

Conclusion Are there evidenced-based criteria to justify the use of HBO in crush injuries and SMCS? When the American Heart Association criteria are used, HBO meets the criteria for a Category 1 indication. With the ten-point evidenced-based evaluation system Ⅰdevised, crush injuries receive seven points and compartment syndromes five points. A score of five points or more qualifies the intervention as evidenced-base.

PL7 The Application of Hyperbaric Oxygen Therapy in Critical Disorders Niu Ko-Chi Department of Medical Research and Department of Hyperbaric Oxygen, Chi Mei Medical Center, Tainan, Taiwan, China

Hyperbaric oxygen therapy (HBOT) has been widely applied in severe clinical disorders. However, HBOT is suggested to be hold when body temperature of the patients is higher than 38.5°C and whether HBO may exaggerate oxi dative stress as results of tissue destruction and subsequent ischemia-induced inflammatory response in critical disorders remained to be investigated.

MATERIAL AND METHOD: In order to study the effect of HBO on oxidative stress in critical condition, we used the animal model of Heat stroke (HS) in Sprague-Dawley (DS) rats to mimic multiple organ failure and high body temperature. Adult male SD anesthetized rats were exposed to an ambient temperature of 43 °C to induce HS. The mome nt in which mean arterial pressure began to decrease from peak levels was arbitrarily taken as the onset of HS. rats were randomized to several groups and given a) no resuscitation (NBA, normobaric air) after onset of heatstroke; b) HBO (100% O 2 at 2.5 ATA for 1 h, 2 h, or cyclic administration); c) hyperbaric air (HBA; air at 2.5 ATA for 1 h); d) normobaric hyperoxia (NBHO; 100% O 2 at 1.0 ATA for 1 h); or e) hyperbaric oxygen 8% (HBO 8%; hyperbaric 8% O 2 at 2.5 ATA for 1 h).

RESULTS: Survival time was 19 ±1, 131 ± 18, 72 ± 14, 68 ± 12, or 45 ± 11 min, (n = 10) respectively for NBA, HBO 1h, HBA, NBHO, or HBO 8% group. The HS-induced arterial hypotension, bradycardia, decreased arterial levels of pH, PaO 2, and SO 2%, increased arterial levels of TNF-alpha, and increased values of cellular ischemia and damage markers, and neuronal damage scores in the cortex were significantly reduced by HBO resuscitation. The cerebrovascular dysfunctions (evidenced by hypotension, intracranial hypertension, and cerebral hypo-perfusion), hypercoagulable state (evidenced by increased values of activated partial thromboplastin time, prothrombin time and D-dimer in plasma), and tissue ischemia/injury (evidenced by increased levels of creatinine, serum urea nitrogen, aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase in plasma, and dihydrobenzoic acid, lipid peroxidation, and oxidized form glutathione/reduced-form glutathione ratio in hypothalamus) that occurred during HS were all suppressed by HBO therapy immediately after the onset of HS.

CONCLUSION: We successfully demonstrated that HBO and to some extent, HBA was found beneficial in resuscitating experimental HS. HBO effectively reduced HS-induced arterial hypotension, plasma TNF-alpha overproduction, and cerebral ischemia and damage and improved survival. The current reuaults indicate that HBO may resuscitate rats that had a HS by decreasing multiple organ dysfunction and brain oxidative stress. We report the case of a patient who displayed a classic HS with multiple organ dysfunction and hypercoagulable state resistant to conventional whole body cooling and antipyretic therapy, necessitating the use of HBOT to rescue from death.

PL8 Hyperbaric Medicine: an European Perspective Daniel Mathieu Centre Hyperbare, Hôpital Calmette, Centre hospitalier Régional et Universitaire, Lille, France

Hyperbaric Medicine has a long history in Europe since the first mention of potential therapeutic application of high ambient pressure for therapeutic purpose goes back to the seventeenth century. Modern application of Hyperbaric Oxygen (HBO) started in the late 1950's in the Netherlands for anaerobic soft tissues infections and in the United Kingdom for carbon monoxide poisoning. Since then, a large number of hyperbaric centers have been implemented throughout Europe, an intense experimental research has been performed and a large clinical work has been done. However, since the mid 1980's and the appearance of the Evidence Based Medicine, European hyperbaric physicians felt the need to improve their organisation and the quality of care delivered by HBO centers. This lead to the development of the "European Committee for Hyperbaric Medicine" (ECHM), which goads are to define commonly accepted indications for HBO, to issue standards for clinical practice, personnel education, technical procedures and safety. There goals were achieved through the organisation of Consensus Conferences, Workshops, reports from ad hoc sub-committees. As a representative body near the European Union, ECHM was successful in promoting an important research program (COST B14) and the issue of a European Norm for therapeutic hyperbaric chambers (EN 14931).

Nowadays, even if not similar everywhere, a typical European Hyperbaric Centers is hospital based and implanted in or near an Intensive Care or an Emergency department and is composed by an air-compressed multiplace hyperbaric system, an hospitalization ward and an out patient clinic. Intensive care patients are commonly treated by HBO and considerable efforts are done to closely monitor treatment effects through, for example, oxygen pressure measurements or blood flow at the targeted spot.

PL9 The pretective effect of Hyperbaric oxygen preconditioning on ischemic/hyoxia injury Xuejun Sun, Hengyi Tao, Weigang Xu, Runping Li Department of diving medicine, Second Military Medical University. Shanghai, China

Hyperbaric oxygen has been widely used as a primary therapy in patients with carbon monoxide poisoning, decompression sickness, and arterial gas embolism, and as an adjunctive therapy for the treatment of various diseases accompanied by impaired oxygen delivery Interestingly, Hyperbaric oxygen has also been tested to produce ischemic tolerance in stroke models and in organs such as spinal cord, heart and liver, suggesting that Hyperbaric oxygen produces a wide scale protective effect, and may be a safer preconditioning stimulus as compared with other stimuli such as hypoxia. Yet the mechanisms underlying its neuroprotective effects remained poorly defined. This paper review the hyperbaric oxygen preconditioning references and our own some experiment results recently. And try give some suggestion for further research in this field.

PL 10 Disruption of Viral Pathogenesis Following Patient Exposure to Hyperbaric Environments with Altered Inspired Gas Mixtures Stephen D. Guthrie, MD, PhD and Barbara R. Guthrie, MD Designed Altobaric  Research Institute, Livonia, Michigan, USA

Introduction There is a broad spectrum of susceptibility to, and virulence of, viral infections. Some of this variance is controlled by the host’s genetics 8,13,25,29,46,50 while some is the consequence of the host’s prior life experiences 43,44,50 . This inconsistency in severity of clinical response to the same infecting agent has prompted many inquires into the innate capacities of the mammalian cell to resist such infections 3,4,9,24,51 . Harnessing the power of these inherent anti-viral mechanisms is a compelling therapeutic stratagem. Further, non-pharmacologic interventions avoid the untoward consequences of all drug-based regimens, these being, direct toxicity, drug interactions, and the universal aftereffect of inducing resistant viral mutations.

The relative intolerance of the infecting pathogen, compared to the infected host, to marked environmental changes 2,6,8,33 suggests an attractive tactic. Saturation divers, residents of undersea habitats and orbiting space stations, and the participants in conventional hyperbaric therapy routinely experience such changes in their ambient milieu. Environmental manipulations producing direct inhibition of viral pathogenesis, or secondary augmentation of immuno-competence, would be effective clinical instruments. The Food and Drug Administration’s first approval of a controlled clinical trial studying HIV/AIDS patients undergoing hyperbaric chamber treatments is a step towards this goal.

Literature Review Relevant publications are categorized either as clinical descriptions of viral infections purposely treated under hyperbaric conditions, or as mechanistic postulations as to how such therapeutic benefits could have occurred.

1. Experimental and Clinical Reports: Using either tissue cultures or experimental animal models, the effects of hyperbaric oxygen exposures on viral pathogenesis appeared in the literature more than 40 years ago 22,38,47 . A wide variety of viral pathogens have been examined including DNA viruses, RNA viruses, and ones with or without envelopes 11,22,44 . Not all types of hyperbaric exposures evoked a positive effect 28 , but consistently the encounter produced some effect on viral penetration, replication, or expression. As early as 1974 experiments were designed to delineate those anti-viral effects caused by the chamber pressure versus those induced by the gas composition 22,28 .

In 1997 a 3-year compilation of individual HIV/AIDS patients treated with hyperbaric oxygen exposures for symptomatic indications documented clinical improvements beyond that which could be expected from their concurrent anti-retroviral treatments 41 . The current FDA trial on HIV/AIDS patients treated with daily hyperbaric exposures is conducted using air 23 before refinement of the inhaled gas mixtures is introduced.

Several individual cases have also been published chronicling improvements in patients with non-retroviral infections such as viral encephalitis 17 and viral hepatitis 20 . Treating the latter clinical condition evolved into a limited prospective study with positive results 30 . The completion of a prospective, randomized trial in viral hepatitis will further our knowledge of whether these particular viruses are inhibited by the marked redox imbalance induced by oxygen under high pressures. 2. Mechanistic Proposals: * Direct virocidal effects: With the possible exception of the tantalizing prospect that nitrous oxide (NO) might have a direct virocidal effect 1,18,49 , most of the gases with proven virus-killing capacity would be too toxic for patient administration 10 . It is possible that gases with virostatic potential could trigger the host’s intrinsic mechanisms for defusing the lethal viral effects 15,44 but that is a speculation without any supportive observations.

* The “membrane” phenomenon: The observed “membrane” effect of gases under pressure is a general biological phenomenon long embraced by hyperbaric medicine 27,48 . Such effects can be at the cell’s surface or within any membrane-encased intracellular compartment. The ion channel effects of gases, particularly inert gases, have been extensively studied in excitable membranes but those same perturbations would occur at all membranes 42 . Particularly intriguing is the finding that xenon, a very inert noble gas at 1 ATA, actually becomes a general anesthetic agent under hyperbaric conditions 32 . Membrane modeling based on the stereoscopic delineation of anesthetic gas-receptor interaction 8 may help select which gases should be studied under pressure. The latest generation of highly active antiretroviral therapy (HAART) drugs specifically inhibits membrane penetration by the virus 36 . This clearly is another battleground wherein environmental composition could be a valuable addition to the therapeutic armamentarium.

* Enhanced immuno-competence: The effects on the immune system of marked alterations in ambient pressure and inhaled gas composition have been known for many years 12 . These particularly have been studied with regard to the reduced immuno-competence observed in long-term occupants of space environments 45 . There are also laboratory observations of increased pathogenicity of certain viruses under hyperbaric conditions 38,47 . Conversely, given the proper pressure/gas combination, viral pathogenicity can be diminished and immuno-competence can be strengthened 20,21,25 . Again, the summary statement here, as with the potential for membrane phenomenon manipulation, is that changes in the host’s ability to address viral infections can be augmented by the ambient conditions. The search must be for that ideal environment, with the supposition that the environment with the greatest viral lethality will likely be determined by the inherent biology of the specific offending virus. * Redox balance manipulation: Another general organism response to hyperbaric environments is imbalance of the redox equilibrium provoked by the reactive oxygen species generated in profusion under such conditions. There are clinical anecdotes documenting viral hepatitis patients’ improvement following hyperbaric oxygen therapy 17,20,30 . Several laboratory models directly implicate the reactive oxygen species as the anti-viral instrument 14,25,31 . These two synchronous lines of investigation have come together as a definitive postulate on disease intervention in the class of enveloped viruses 6. Further investigation will reveal if the reactive oxygen strategy can be employed in all viruses, or only certain classes, or at only certain vulnerable watershed moments in the course of viral infections. As clinicians, the patient trial processes should be started even as laboratory investigators pursue the intricate mechanisms whereby these beneficial effects occur.

* i RNA mechanism(s) The mechanism, or more properly the family of mechanisms, that act to derail, mute, or inactivate the pathologic genetic material introduced by a virus have been formally denominated the “silencing interference RNA” system. Several acronyms exist (si RNA, iRNA, RNA i) all alluding to this same process. The most compelling scientific information from a clinical standpoint derives not from bench-top laboratory experiments or patient treatment examples but from descriptions of genetic variances which have resulted in individuals 29,35 , or whole populations 13,46,50,51 , having the inherent capacity to “ignore” the fatal viral infusion. In a word, those fortunate cells or individuals are capable of “silencing” the fatal genetic instructions. Many of these fortunate adaptations have likely evolved over millennia 50 but they may appear spontaneously, and quite suddenly 29 . There are vigorous attempts to harness the si RNA mechanism(s) for immediate clinical utility, and there are sophisticated laboratory tools for quantifying changes in these processes. It should at least be born in mind that accessing changes in the si RNA system(s) could be done contemporaneously with the other clinical trials suggested here.

Research Protocol In April of 2005 the Food and Drug Administration approved the submitted protocol for, and issued permission to proceed with, a Phase-II Clinical Trial on the effects of hyperbaric exposures in patients with HIV/AIDS 23 . That regulatory body thus accepted the results of the Phase-I study on healthy volunteers 40 . Further, that approval signaled the FDA’s recognition of the scientific premise that such altered environments would positively influence the progress of this disease based on published laboratory experiments and clinical case reports. The FDA’s assessment of the proposal and protocol allowed them to assign a “Non-Significant-Risk” (NSR) categorization to this trial. This NSR designation obviates inclusion in the study of components assessing sensitivities and allergies, drug interactions, and risks associated with concurrent medical conditions or treatments. The results of this Phase-II study will determine the design and sample size of the Phase-III trials to determine final efficacy.

Discussion Any definitive discourse must await the results of the Phase-II Clinical Trial. In the absence of those results any discussion here ought simply to declare, and reinforce, the reasons why this study is proceeding. Vigorous and colorful discussions will undoubtedly follow completion of this trial, discussions regarding the meanings, the mechanisms, and the direction(s) of the next investigational steps. For the moment, the following assertions should be added to the working body of conversation in this discipline:

1 – Ambient pressure changes and altered inspired gas mixtures have a demonstrable effect on the clinical course of patients with viral infections, both through effects on the invading pathogen and on the host’s ability to resist that insult.

2 – Both redox imbalances and inert gas membrane effects can produce such effects. The design of the prescribed gas/pressure blend may depend on the biological characteristics of each individual pathogen.

3 – Such environmental manipulations are spared many of the untoward consequences of drug-based therapeutic regimens. Further, the potential synergy between hyperbaric treatments and administered medicaments could lessen the emergence of resistant strains of the virus that follows use of each drug.

It is fortunate that studies on environmental manipulations are not encumbered by the unwelcome physical liabilities linked to pharmacological interventions. Further, it is fortunate that Hyperbaric Medicine has the talent, the intent, and the clear mandate to extend the borders of this therapeutic modality.

PL 11 Carbon Monoxide Poisoning Clinical Findings, Common Errors, And Early diagnose A prospective analysis of 2900 cases Jordi Desola, MD, PhD, Joan Sala, MD. Hyperbaric Therapy Unit of Barcelona, Barcelona, Spain

Carbon Monoxide Poisoning (CMP) is one of the best documented indications for HBO, where many research has been done, and important experience has been cumulated from all the world. In spite of that, the general opinion of the medical community is not unanimous and it remains confused. Results are really encouraging, but some sceptical attitude about the role of hyperbaric oxygen (HBO) still remains among some hospitals in the area. Many of these attitudes can be explained by a lack of knowledge of the toxicology, pathophysiology and clinical pattern of this frequent inhaled intoxication. The majority of the cases in Catalonia happened within the domestic environment, so the role of primary assistance teams can be essential. However we have observed a large series of common errors, both in the primary assistance medical centres as well in the most important general hospitals, which being so repetitive can not be considered as casual. We have analyzed our series of near to 3000 cases of CMP received in our unit, and we are pointing out the most frequent and relevant errors.

1.DELAYED DIAGNOSIS . Its condition of tasteless, colourless, and odourless, can maintain Carbon Monoxide (CO) hidden once the source is extinguished or simply the windows of the room are open. In such condition, erroneous diagnoses are frequently established in urgency departments and primary assistance cabinets, like Encephalitis, Stroke, Epilepsy, Dysbaric disorder, Tensional Cephalalgia, Occupational conflict, Collective Hysteria, Food poisoning, or Peripheral Vertigo.

2.CONFUSION ASPHYXIA/ POISSONING . CMP is very often a hidden disaster. Only in catastrophic situations, or in cases where coal gas is obviously present, the existence of CO in the environment is presumed. Liquated gases from petroleum (propane, butane, and so on) do not contain CO so they are not toxic. However in case of free escape the inhalation of propane or butane can produce serious asphyxia, but not a poisoning. If the combustion of these gases is incomplete, CO can be formed. In such cases signs and symptoms of asphyxia and CMP will combine and careful attention is needed to associated disorders like smoke inhalation, or Adult Distress Respiratory Syndrome.

3. ERRONEOUS EVALUATION OF CARBOXYHAEMOGLOBIN LEVEL . Even when CMP is almost certain, some doctors only accept this diagnosis if the carboxyhaemoglobin (HbCO) percentage is elevated. And even in these cases, they only consider intoxication as severe if the HbCO elevation is very high. This attitude implies a true ignorance about both the pathophysiology of this intoxication, as well as the physiology of the haemoglobin oxygen transport and the carbon monoxide left deviation of the curve. HbCO only indicates the haemoglobin linked to CO, but nothing is known about the plasmatic CO levels. However, it is well known that in mild cases, patients often have important HbCO elevations, while other patients in deep coma can have low levels. An HbCO elevation only informs that the patient has been exposed to CO at some point during the preceding hours, but neither a correlation with severity nor with prognosis can be established. It is frequently forgotten that the worst toxic role of CO depends on the direct toxic effect over kytochrome-oxydase mitochondrial breathing.

4.ERRONEOUS EVALUATION OF GASOMETRICS AND PULSE-OXIMETRY . Patients poisoned by CO often have nearly normal gasometrical values. A mild hypoxemia may be sometimes observed even in seriously ill patients. Gas blood analytical machines only detect pH, haemoglobin, and arterial pressure of Oxygen and Carbon Dioxide. The other parameters like Bicarbonates, Base Excess, or Oxygen Saturation are calculated -not measured- so when an important quantity of haemoglobin is blocked by CO, the results shown by conventional machines are erroneous. Only Cooxymeters can determine the real oxygen content measuring the actual Oxygen Saturation, but these apparatus are not always available in small hospitals, and almost never in primary assistance medical centres. Pulse-oximetres are really a kind of photocolourimetres and they give erroneous results in case of CMP as well. The recently appeared Pulse-cooximetres can be the most valuable aid in the early diagnose of CMP especially on site for rescuers, first responders, or primary assistance specialists.

5. IMPROPE ESTIMATION OF SIGNS AND SYMPTOMS . Signs and symptoms of CMP are very unspecific. Cephalalgia, Nausea without vomiting, Bradipsyquia, Asthenia, and transitory Consciousness Disturbance are the most common in the not extremely serious cases, which are the most frequent. Some books of internal medicine and on toxicology, explain that one of the most typical symptoms of CMP is the Sherry skin rush, while in our series it has been found in only the 20.3% of the cases. On the contrary, some recent papers consider the Sherry skin rush as a very rare finding. It is obvious that the majority of the patients are not aware of this sign thus they do not report it, and it can remain hidden if it is not actively searched in the emergency department.

6. INNECESSARY CORRECTION OF METABOLIC ACIDOSIS . These patients are usually in real acidotic condition, which may be more related to the severity of the intoxication than the HbCO level. First responders, and urgency physicians in hospitals sometimes try to "correct" this acidosis, and their patients become alkalotic after having received unnecessary doses of bicarbonates. Metabolic acidosis is mainly related to the left deviation of the haemoglobin curve, and it can become a kind of defence against hypoxia trying to achieve a more stable oxygen transference. A moderate acidosis is a positive event and need not to be a concern. Only extreme acidosis in the most severe cases, which can be by itself a cause of major damage, need to be corrected.

7.NON JUSTIFIED ADMINISTRATION OF HYDROXICOBALAMIN . Cyanide (CNH) can be formed in cases of fire in closed environment, very high temperature, and combustion of synthetic and plastic materials. Following the so called “Baud’s criteria” a combined CO plus CNH poisoning can be assumed in some cases of patients rescued from fires. Some experimental laboratory data, not clinically controlled, suggest that very high doses (between 2-5 grams endovenously) of Hydroxicobalamin (HC) can be the antidote of CNH. This overestimated measure has become very popular in some areas and first responders have incorporated HC among their emergency procedures. However HC has no action over the mitochondrial breathing disturbance since only blocks the inhaled CNH, but the patient will persist hypoxic if high quantity of oxygen is not given. Furthermore, HC has no action over CO, so there is no reason to apply in pure cases of CMP in which no criteria of CNH poisoning are estimated, and on the other hand HC is very expensive and it can delay the application of the only etiological treatment with HBO.

8.OVERESTIMATION OF THE UTILITY OF NORMOBARIC OXYGEN . Many patients start to improve from the moment of the rescue from the intoxication site. In fact, they frequently arrive at the hospital in nearly normal status. Applying normobaric oxygen may result in an apparent improvement in the less serious cases, but this amelioration does not correspond to a real healing. High quantities of normobaric oxygen can moderately increase the oxygen concentration in the blood, but the oxygen deliver to the cell will be always done under atmospheric diffusion pressure. Theoretically, the half life of HbCO breathing normobaric oxygen is 80 minutes. But in practice some patients have received normobaric oxygen for several hours in primary hospitals previously to be sent to our unit, and the mean value of HbCO in the moment of the arrival to the emergency room of our hospital is still high.

9.DELAY OR NEGLIGENCE IN APPLYING HYPERBARIC OXYGENATION . The majority of the guidelines or protocols in Emergency department of general hospitals are still conditioning the indication of HBO for CMP patients to HbCO values over 20% or even over 40% what implies a lack of knowledge of the real pathophysiology of CMP and even of the toxicology of CO. Thus physicians from emergency rooms wait for the result of determination of HbCO before deciding whether or not the patient must be sent to the Centre of Hyperbaric Medicine. The delay in our area since the moment that patients arrive to the hospital until they arrive to our unit is near to 7 hours. And many seriously poisoned patients are not sent to Hyperbaric medical centres simply because that at this time the HbCO is already low, in spite of the fact that the patient has suffered a serious poisoning.

10. IGNORANCE OF THE LATE NEUROLOGICAL SYNDROME . Many general practitioners and hospital doctors are unaware of the simple existence of this serious neurological demyelinization syndrome that appears after some days of fully symptomatic remission. Not only practitioners but some hospital neurologists are equally uninformed. Several well known and internationally accepted textbooks on internal medicine, and even some books on toxicology do not mention the risk of late neurological symptom in case of CMP or it is described as a neurological sequella of the acute intoxication..

This was not an exhaustive list of common errors committed by both practitioners from primary assistance as well as doctors from emergency departments in General hospitals. Many of them are based in a lack of information of some aspects that are well described in basic textbooks of respiratory physiology. Some other errors depend on a sceptic attitude proning to accept any drug treatment but at the same time very reluctant against not pharmacologically supported treatments. Data is nowadays relevant. Research is deep and wide. But there are no more serious blinds than those not wishing to see.

投稿发言 Free Paper Abstract

OR01 HBO and cytostatic chenotherapy OR03 Christtian Heiden Hyperbaric Oxygen for Complications of Oncological German Professional Soc. Hyperb. Chambers Treatment of Paediatric Patients Michal Hajek 1, Pavlina Cahova 2, Jaroslav Sterba 2, Jiri Objective: There is a certain amount of reservation for Ruzicka 3, Pavel Zonca 4 applying HBO to patients who have a history of malignant tumor treated with cytostatic drugs. As hypoxic tumor cells 1. Centre of Hyperbaric Medicine, Municipal Hospital of do not react well to treatment wether it is radiotherapy or Ostrava, Czech Republic, 2. Clinic of Paediatric Oncology, cytostatic drug there is a ratio for applying HBO as an Faculty Hospital of Brno, Czech Republic, 3. Faculty adjuvant for cytostatics. Method: A survey of the literature Hospital, Charles University of Pilsen, Czech Republic, 4. on this topic is given in detail, also concerning the different Centre for Visceral and Mini-invasive Surgery, classes of cytostatics. Own experience of the last 5 years Krankenhaus Wesseling, Köln am Rhein, Germany is presented. Results: Few publications deal with HBO and cytostatics. There are encouraging papers published. Introduction: Use of Hyperbaric Oxygen Treatment The number of cases treated with HBO after cytostatic (HBO) for children requires special knowledges for tumor therapy and even so as an adjuvant to cytostatics is indication and treatment. Especially nursing of critically ill very limited and far beyond evidence based medicine. In patients demands specific equipment inside of hyperbaric all our own cases the HBO treatment did not cause any chamber. There is a strong need for close cooperation problems. Conclusion: The application of HBO after between paediatric and hyperbaric specialists for cytostatic treatment is feasable in general. Precautions ensurance of adequate care of sick paediatric patients. have to be made concerning special substances. As the HBO for sick oncological patients is very exhausting for experience published is very small the adjunctive nursing staff from psychological, organizing as well as application of HBO should be limited to very special cases treatment point of view. There is a long tradition of after all treatment options have failed and the patient treatment of children within our treatment centre with would be lost otherwise. Informed consent of the patient multiplace hyperbaric chamber equipped with perfect for the treatment is indispensable. monitoring, ventilation and technical devices, as our hyperbaric center started to work in 1965 and from that time more than 16.000 patients were treated here. Methods and Results: This is retrospective OR02 observational study. From 1994-2008 total of 33 children Hyperbaric Oxygen: Does it Promote Cancer (19 of male sex, 14 of female sex) have been treated for Recurrence or Metastasis? An Update complications of oncological treatment in our hyperbaric John Feldmeier unit. In the early period the hepatotoxic influence of University of Toledo Medical Center; Radiation Oncology cytostatic drugs has been the main reason for HBO Department; Toledo Radiation Oncology, USA treatment (17 patients). Recently hyperbaric oxygen is used due to severe posthypoxic encephalopathy(3 Objective: To review and update the literature related to patients), after extensive intracranial surgery for malignant cancer growth, recurrence and metastases after diseases(9 patients) with different grade of brain damage hyperbaric oxygen. Methods A comprehensive review of and neurological deficit, complications due to treatment this topic was published by Feldmeier et al in 2003. Since of immunity suppressive drugs (sepsis, critical illness that time several new publications have been added to the polyneuromyopathy- 3 patients). One children has been body of literature on this topic. The prior review as well treated for radiation injury of urine bladder. Mean age of and the more recently published literature, found by children were 8 years (1-17 years), 22 patients were computer based literature search (Medline), are reviewed. treated for blood malignancy, 11 patients for solid tumour. Results: The literature on this topic can be divided into 1) Mean number of HBO sessions were 7,5 (1-20), Case reports and case series; 2) Randomized trials treatment pressure of 1.9 to 2.5 ATA. Among of them, designed to investigate hyperbaric oxygen as a positive effect has been achieved in 89 to 100 % of cases radiosensitizer; and 3) Pre-clinical studies designed to due to clinical situation, total positive treatment effect was address the issue in question and encompassing a broad described in 31 from 33 patients(94 %).One patient out of range of malignancies. Only a rare anecdotal case or 33 (3%) could not finish his treatment due to ear small case series reports cancer recurrence or equillibration problems, in two patients bleeding from progression after hyperbaric oxygen. Much larger case tracheostomy as well as nose occured, but without need reports fail to confirm a higher rate of cancer recurrence. of interruption of HBO sessions, one patient (3%) failed to The randomized trials from the era of hyperbaric oxygen achieve positive tendency from treatment. Discussion: radiosensitization do not suggest an increased risk for This is a pilot study for creating of basal imagine of HBO metastases or death due to cancer after hyperbaric effects in those clinical situations. It is very important to oxygen. The vast majority of pre-clinical studies show emphasize that it is a small group of patients, evaluation is either a neutral effect or a slight suppression of malignant provided retrospectively and without use of any standard growth. Conclusions: The preponderance of available measurement method and parameters. Most of patients evidence fails to demonstrate an enhanced rate of cancer can be evaluated as criticallly ill patients and the complex growth, recurrence or metastasis. Hyperbaric oxygen can treatment has been supplied. HBO treatment for those be given to cancer patients without fear that the treatment patients is high risk due to infectious diseases, immune will promote cancer recurrence or progression. suppression therapy, need for isolation of patients inside of hyperbaric facility. We agree with the presence of 2. Department of Cardiac Surgery, Collegium Medicum, parents during HBO treatment. ENT examination with Nicolaus Copernicus University, Bydgoszcz, Poland Odstraněno: ¶ paracenthesis, or bilateral tympanostomy with ventilation tubes insertion within general anesthesia are provided Objective: Postsurgical complications after sternotomy Odstraněno: HBO in Tokyo before of HBO treatment. For assessment of HBO include mediastinal dehiscence and mediastinal wound Medical and Dental treatment there have been used standard neurological infection, either superficial or deep. Mediastinitis, as a University in 2007 and The examination, electromyography(EMG), or creatin kinase deep wound infection, is a rare complication after the Effects of HBO on Soft levels in blood. Conclusion: Objective evaluation of surgical sternotomy, but it can have high mortality. Tissue Injury in Sports mechanisms of HBO effects and confirmation of its Several publications show the positive effect of usage of Activity¶ positive acting will demand next studies, moreover with the hyperbaric oxygenation (HBO) in mediastinitis with Kazuyoshi Yagishita, Nobuo randomised prospective design. Present results show that shorter hospital stay, less morbidity, and cost saving. Yamami, Seiichiro Togawa, HBO can be useful method in treatment of paediatric Methods: In the National Center of Hyperbaric Medicine Yoshihiro Mano ¶ oncological patients with neurological deficit and other in Gdynia, Poland, between 2005 and 2008 we treated Hyperbaric Medical Center, oncological treatment complications. with HBO thirteen patients (7 males and 6 females) aged Tokyo Medical and Dental between 53 to 74 years (mean 60.5±6.9 [SD] years) with University, Tokyo, Japan¶ an open mediastinum due to mediastinitis in ¶ post-operative period after medial sternotomy for cardiac Objective: In our university OR04 surgery. In one case there were clinical and hospital, multiplace Hyperbaric Oxygen may accelerate tumour-bearing microbiological evidences of clostridial mediastinitis. chamber including 3 rooms mice to death Patients were admitted into the hyperbaric center after and the capacity of 16 Qingle LIU, Chenggang Zheng, Xiaohua Hang initial surgical debridement of wounds and removal of patients was set up in 2001. Changhai Hospital, the Second Military Medical University, sternal wires. Standard care included: daily change of In 2007, 7970 times Shanghai, China dressing with wound irrigation and debridement, when hyperbaric oxygen therapy necessary; narrow spectrum antibiotics; HBO sessions (HBO) in 958 patients were Objective: To explore the influence of hyperbaric every 12 hours until resolution of infection; adjunctive performed, which is the oxygenation (HBO) on life-span in tumour-bearing treatment as indicated by the clinical status of patients. most patients number in one mice. Method: Male Balb/c mice were randomly divided Results: Median length of stay in the hyperbaric center institute in a year in Japan. into celiac-tumour group: inoculating tumour cell into was 12 days (range 9-38), median number of HBO The purposes of this study abdominal cavity of the animal, celiac-tumour-HBO group: sessions was 20 (range: 16-54). Median value of were to report the diseases inoculating tumour cell into abdominal cavity and then C-reactive protein (CRP) titer at admission was 79.8 mg/dl and the patients performed exposing to hyperbaric oxygen, back-tumour group: (range: 12.8-260.8) and significantly decreased down to with HBO in our hospital last inoculating tumour cell under skin of the back of the 12.8 mg/dl (range: 7.8-103.1) in all patients (p=0.005; year. The effects of HBO on animal, back-tumour-HBO group: inoculating tumour cell Wilcoxon test). Median value of WBC count at admission crush injury and under skin of the back of the animal and then exposing to was 7470/ml (range: 5100-18670) and it did not change compartment syndrome hyperbaric oxygen. There are 7 mice in each group. After significantly at discharge (7420/ml; range: 2870-10890; were well documented by s-180 tumour cell was translated to mice HBO was given p=0.12; Wilcoxon test). In all cases there was many authors, and once a day in concerned group. The life-span and skin improvement in the local and general clinical status and in randomized controlled ulcer were observed. Results: The life-span is 27.57 士 microbiological results, even if not always there was a studies revealed the effects bacterial eradication. No major complication occurred of HBO on reduction of 4.4693 days in celiac-tumour-group, 24 2.9439 days in 士 during the HBO sessions. Conclusion: Usage of HBO necrosis and edema. We celiac-tumour-HBO group, 63.43 士 21.844 days in should be considered in mediastinitis as a complication of now perform HBO the cardiac surgery. aggressively to soft tissue back-tumour group, and 35.14 8.934 days in 士 injury including back-tumour-HBO group. The life-spans in celiac-tumour compartment syndrome, group and in celiac-tumour-HBO group are different, but P ankle sprain, knee ligament value is 0.133204; the life-spans in back-tumour group OR06 injury, and muscle strain. and in back-tumour-HBO group are significant and P Normobaric and hyperbaric oxygen therapy for The purposes of this study value, 0.009002. Conclusion: The life-span of mice in migraine and cluster headache were also to investigate the back-tumour-HBO group was shorten by exposing HBO, Michael Bennett 1, Christopher French 2, Alexander 3 4 3 effect of HBO on soft tissue and life-span in celiac-tumour-HBO group is tendency to Schnabel , Jason Wasiak , Peter Kranke injury in sports activity. be shorten by HBO exposure. The ulcer formation is 1. Dept. Diving and Hyperbaric Medicine, Prince of Wales Patients and Methods: postponed by HBO in back-tumour-HBO group. Hospital, Australia 7970 times HBO in 958 2. Dept. Neurology, Prince of Wales Hospital, Australia patients were performed in 3. Dept. Anaesthesiology, University of Wuerzburg, our hospital in 2007. The Wuerzburg, Germany number of the patients and 4. Victorian Adult Burns Service, The Alfred Hospital, HBO times were evaluated. Melbourne, Australia In 180 patients with soft OR05 tissue injury in sports activity, Hyperbaric oxygenation (HBO) in mediastinitis treated Background: Migraine and cluster headaches are severe visual analog scale (VAS) with an open mediastinum after cardiac surgery and disabling. Migraine affects up to 18% of women 1 1 scores and recovery time to Jacek Kot , Zdzislaw Sicko , Lech and cluster headaches 0.2% of the population. A number previous sports activity were Anisimowicz 2, Wojciech Pawliszak 2 of therapies are available, including both hyperbaric evaluated. Results: The... [27] 1. National Center for Hyperbaric Medicine, Medical oxygen (HBOT) and normobaric oxygen (NBOT). We University, Gdansk, Poland assessed the safety and effectiveness of both for treating Odstraněno: ¶ and preventing migraine and cluster headaches. Methods: manufacturer’s instruction. Results: Intravenous We searched the following in May 2008: CENTRAL, administration of lipopolysaccharide (2 µg/kg) caused MEDLINE, EMBASE, CINAHL, DORCTIHM and increased levels of both core temperature and reference lists. Relevant journals were hand searched hypothalamic glutamate, hydroxyl radicals, and and researchers contacted. We accepted any randomised prostaglandin-E2 accompanied by increased plasma trials comparing HBOT or NBOT with any levels of tumor necrosis factor-α, interleukin-1β, and alternative. Three reviewers independently evaluated interleukin-6. Treatment with hyperbaric oxygen (100% at study quality and extracted data. Results: Nine trials 253 kPa) once a day for consecutive 7 days prior to or 1 involving 201 participants were included. Pooling of data hour after injecting lipopolysaccharide significantly from three trials suggested HBOT was effective in reduced the lipopolysaccharide-induced elevation of core relieving migraine headaches compared to sham temperature, circulating tumor necrosis factor-α, therapy (RR 5.97, 95% CI 1.46 to 24.38, P = 0.01). There interleukin-1β, and interleukin-6, and hypothalamic was no evidence that HBOT could prevent migraine, or glutamate, hydroxyl radicals and prostaglandin E2. Direct reduce the incidence of nausea/vomiting or the injection of tumor necrosis factor-α (20 ng), interleukin-1β requirement for rescue medication. There was a trend to (20 ng), or interleukin-6 (10 ng) into the lateral cerebral better outcome in a single trial evaluating HBOT for ventricle also caused a rise in both core temperature and cluster (RR 11.38, 95% CI 0.77 to 167.85, P = 0.08). hypothalamic glutamate and hydroxyl radicals, which NBOT was effective in terminating cluster headache could be attenuated by treatment with hyperbaric oxygen. compared to sham in a single small study (RR 7.88, 95% Conclusion: Hyperbaric oxygen can be used as a CI 1.13 to 54.66, P = 0.04), but not superior to ergotamine prophylactic as well as a therapeutic agent for prevention administration in another small trial (RR 1.17, 95% CI 0.94 or suppression of endotoxin-related systemic to 1.46, P = 0.16). Seventy-six per cent of patients inflammation and fever in rabbits. responded to NBOT. No serious adverse effects of HBOT or NBOT were reported. Conclusion: There was evidence that HBOT was effective for the termination of migraine in an unselected population, and weak evidence that NBOT was effective in cluster. Given the cost and OR08 poor availability of HBOT, more research should be done Measurement of plasma nitrotyrosine and nitrite after on patients unresponsive to standard therapy. NBOT is a single treatment with hyperbaric oxygen in healthy cheap, safe and easy to apply, so will probably continue to human subjects be used. Renu Agnihotri, Amy Welch, James Stewart, Dheeraj Bansal Saba University School of Medicine, Netherland Antilles

OR07 Recent data suggest nitrite as a product of the complex Hyperbaric oxygen therapy induces antiinflammation interplay between nitric oxide (NO), molecular oxygen and and antipyresis in experimental studies superoxide that takes place during hyperbaric oxygen Lin Mao-Tsun 1, Gao Chunjin 2, Niu Ko-Chi 1 therapy (HBOT). A rapid reversible decrease in 1. Department of Medical Research and Department of endothelial NO bioavailability during hyperbaric oxygen Hyperbaric Oxygen therapy, Chi-Mei Medical Center, exposure may possibly be due to this auto-oxidation of Taiwan, China NO. Inorganic anions such as nitrite that were previously 2. Department of Hyperbaric Oxygen Therapy, Chaoyang thought to be inert end products of endogenous nitric Hospital Affiliate of Capital University of Medical Sciences, oxide metabolism are now being viewed as possible Beijing, China storage pools of NO-like activity. With the reduction of nitrite back to NO in the circulation indicated as a possible Objective: To ascertain whether hyperbaric oxygen mechanism for hypoxic vasodilatation, nitrite is being therapy inhibits the increase of glutamate, hydroxyl investigated as an endogenous signaling molecule and radicals, and prostaglandin E2 in the hypothalamus and regulator of gene expression. Given its role in blood flow reduces fever during lipopolysaccharide-induced systemic regulation and hypoxic nitric oxide homeostasis, nitrite inflammation in rabbits. Methods: Adult male New may not only serve as a diagnostic marker but also find a Zealand white rabbits, weighing between 2.2 and 3.2 kg at role as a potential therapeutic agent. Protein nitrotyrosine the start of the study, were used. The pyrogen assay was has been identified as a marker of formation of another carried out with unanesthetized animals restrained in potent oxidant, peroxynitrite from nitric oxide. Objective: rabbit stocks. The microdialysis probes were To examine changes in the nitrite and peroxynitrite stereotaxically and chronically implanted into the preoptic metabolites of NO in the blood of healthy subjects after a anterior hypothalamus of rabbit brain (the essential single HBOT session. Method: Fifty six healthy adults thermoregulatory center) for assessment of glutamate, were included in the study. Plasma nitrite was measured hydroxyl radicals, and prostaglandin E2 in situ. For in 22 test subjects and 12 controls before and one hour measurement of serum cytokines, 5 ml of blood was after HBOT with either 100% oxygen or compressed air at withdrawn from the marginal ear vein of each rabbit. The 2.5 ATA for sixty minutes. Determination of plasma amounts of the cytokines tumor necrosis factor-α, nitrotyrosine was done separately with 15 test subjects interleukin-1β, and interlueimin-6 in the serum were and 7 controls using the same protocol. Results: Plasma determined by using double-antibody sandwich ELISA nitrite and nitrotyrosine levels were not found to be (R&D systems, Minneapolis, MN, USA) according to the significantly altered after the single HBOT in subjects who received 100% oxygen therapy as compared to OR10 compressed air. There was a non-significant elevation of Adjunctive use of hyperbaric oxigenacion (hbo2) in Odstraněno: OR09 ¶ plasma nitrites and nitrotyrosine in both groups of HBOT the managment of cerebral hemorrhage in a pregnant Hyperbaric Oxygen may subjects as compared to controls that received no HBOT. patient: a case report. accelerate tumour-bearing Conclusion: Increased oxygenation of tissues due to Chávez A., Uribe R., Sánchez EC. mice to death ¶ hyperbaric therapy may therefore be more significantly Hospital Angeles Metropolitano, Mexico Qingle LIU, Chenggang Zhe related to the production of reactive metabolites of nitric ng, Xiaohua Hang oxide than the oxygen content of the inhaled air, in short Changhai Hospital, the term HBOT. Introduction: The purpose of a craniotomy is Second Military Medical decompress through a cranial bone resection. It does not University, Shanghai, China reverse the primary injury, but reduces secondary ¶ damage caused by cerebral edema after a hemorrhagic Objective: To explore the OR09 vascular event. The association of HBO is beneficial if it is influence of hyperbaric HBO in Tokyo Medical and Dental University in 2007 performed in the early stage. Material and Methods: We oxygenation (HBO) on and The Effects of HBO on Soft Tissue Injury in reported a 33-year-old female patient who has a cerebral life-span in tumour-bearing Sports Activity hemorrhage secondary to an arteriovenous malformation. mice. Method: Male Balb/c Kazuyoshi Yagishita, Nobuo Yamami, Seiichiro Togawa, The case is complicated because the patient has a 25 mice were randomly divided Yoshihiro Mano weeks pregnancy. HBO (1.8 atm abs / 90 min / QD / 20 into celiac-tumour group: Hyperbaric Medical Center, Tokyo Medical and Dental treatments) was used a adjunctive treatment as an early inoculating tumour cell into University, Tokyo, Japan neuroprotector to inhibit ischemia reperfusion injury. MRI abdominal cavity of the was done before and after HBO. Results: The patient animal, celiac-tumour-HBO improved clinically and by MRI. There was a marked group: inoculating tumour Objective: In our university hospital, multiplace chamber improvement of the motor and cognitive function. The cell into abdominal cavity including 3 rooms and the capacity of 16 patients was set patient was able to maintain the pregnancy that was and then exposing to up in 2001. In 2007, 7970 times hyperbaric oxygen resolved by Caesarean section. There was no secondary hyperbaric oxygen, therapy (HBO) in 958 patients were performed, which is effect of HBO to the neonate. Conclusion: This case was back-tumour group: the most patients number in one institute in a year in complicated not only by the cerebral hemorrhage of the inoculating tumour cell Japan. The purposes of this study were to report the mother but also by a 25 week pregnancy. HBO was used under skin of the back of the diseases and the patients performed with HBO in our as an early neuroptotector. It inhibits ischemia reperfusion animal, back-tumour-HBO hospital last year. The effects of HBO on crush injury and injury (I / R) when used at an early stage. There was a group: inoculating tumour compartment syndrome were well documented by many marked clinical and MRI improvement of the mother and cell under skin of the back of authors, and randomized controlled studies revealed the there were no secondary effects to the product, specially the animal and then effects of HBO on reduction of necrosis and edema. We retinopathy of the premature. This is the first case of the exposing to hyperbaric now perform HBO aggressively to soft tissue injury used of HBO for cerebral hemorrhage in a pregnant oxygen. There are 7 mice in including compartment syndrome, ankle sprain, woman. each group. After s-180 kneeligament injury, and muscle strain. The purposes of tumour cell was translated this study were also to investigate the effect of HBO on to mice HBO was given soft tissue injury in sports activity. Patients and Methods: once a day in concerned 7970 times HBO in 958 patients were performed in our OR11 group. The life-span and hospital in 2007. The number of the patients and HBO Necropsic findings of syndrome of intratoracic skin ulcer were times were evaluated. In 180 patients with soft tissue hyperpressure in a young diver and a decompression observed. Results: The injury in sports activity, visual analog scale (VAS) scores accident after a deep scuba diving in veteran diver life-span is 27.57 士 4.4693 and recovery time to previous sports activity were (two particular cases) evaluated. Results: The number of the patients and HBO Batle J.M. 1, Poncela J.L. 2, Jimenez M.A. 2 days in celiac-tumour-group, times in decompression illness were respectively 374 1. MEDISUB Hyperbaric Research Institute, Palma de 24 士 2.9439 days in patients and 764 times, 87 patients and 934 times Mallorca, Spain celiac-tumour-HBO group, insudden deafness, 34 patients and 740 times in 2. Legal Medicine Institute, Palma de Mallorca, Spain 63.43 士 21.844 days in peripheral vascular disorder including diabetes and arteriosclerosis obliterans, 47 patients and 1046 times in Introduction: On the coasts of our islands, (the Balearic back-tumour group, and osteomyelitis, 180 patients and 865 times in soft tissue Islands), the cleanliness and the almost constant 35.14 士 8.934 days in injury related sports activity, 90 patients and 1644 times in temperature of the waters all year round , creates a great back-tumour-HBO group. myelopathy and radicuropathy, 19 patients and 220 times interest and a great number of followers to sub-aquatic The life-spans in in carbon monoxide poisoning, and 18 patients and 259 activities either of a professional or recreative character. celiac-tumour group and in times in radiation-induced cystitis. In patients with soft The depths of the Mediterranean sea ranges from 50 to celiac-tumour-HBO group tissue injury in sports activity, VAS scores improved 120 metres with the existence of red coral colonies are different, but P value is compared between pre and post 2-hour HBO. In patients highly valued in Jewellery shops. The Government 0.133204; the life-spans in nd with 2 grade medial collateral ligament injury of the knee controls the above mentioned professionals limiting the back-tumour group and in in HBO group, recovery acceleration to previous sport number of permits or authorizations to only a few and back-tumour-HBO group are activity was observed. Conclusion: In HBO procedure in these boats are usually worked by a Captain and a sailor. significant and P value, our university in 2007, no major patient troubles and no Materials and Methods: 12 year old patient while diving 0.009002. Conclusion: The chamber problems were recorded. The effects of HBO on and making an uncontrolled ascent suffered a life-span of mice in soft tissue injury were strongly suggested in this study. Intrathoracic Overpressure Syndrome and an Arterial back-tumour-HBO group Gas Embolism. The professional divers are in the habit of was shorten by exposing diving depths from 80 to ll0 metres using scuba breathing HBO, and life -span in ... [28] equipment with three bottles of 3 x l8 litres and lighting received lidocaine, and required between 0 and 12 equipment of l50w. besides a bag for the gathering of sessions of hyperbaric oxygen therapy (HBO). Of the the coral . They are in the habit of hanging this from the patients that did not receive lidocaine, three did not neck and the pickaxe is hung from the right hand. The require more sessions and the rest required between five divers make decompression stops from 51 metres and are and ten sessions. The only patient who had a complete ascending gradually, when they are at l2 metres they go spinal disease required 25 sessions of HBO. From the to surface where they have a decompression chamber cases that had a cerebral disease, two presented with installed in their boat, to continue their period of alterations in consciousness. One of them died in spite of decompression returning to pressure again of 12 hyperbaric treatment and ICU treatment. The other had an metres and to make the corresponding stops. To make alteration in consciousness and myoclonus and survived the step of humid decompression to decompression in without sequels. The other two cerebral cases had hyperbaric chamber, the surface maximum time is very lacunar syndromes that recovered completely with limited to a maximum of 5 minutes. The 74 year old hyperbaric treatment. Of the 7 cases of peripheral disease, patient who after making his dive to 84 meters of depth 6 of them had hypoestesia in the territory of the circumflex was 20 – 25 minutes on the bottom and his nerve, associated with articular and muscular compromise. decompression stops lost conscience inside the Conclusion: A high percentage of the treated cases of decompression chamber installed on board. The decompression illness are NDCI. Possibly these cases materials used are the usual ones of the Medical Legal are overrepresented. The presence of a “dissociation” or Institute and two cameras. The methodology was the existence of different patterns of injury in the spinal cord system used in all cases of death, not natural with could be explained because of different physiopathologic technologies and specific enquiries for these cases. reasons, that is to say, arterial embolization in cases of Results: The Signs that appeared in the case of SIHP spinal disease with respect of the posterior columns, were immediate at the arrival to the surface and were a venous thrombosis in cases of a complete medullary neurological type. The findings found the levels of the syndrome, and presence of bubbles in cases of a not different organs which have always been described in systemizable spinal cord disease. The pattern of complete communications and literature are evident and in these spinal disease seems to have a worse prognosis. All cases, though it is necessary to emphasize certain organs patients had important omissions of decompression. reveal that they have still not been described. Conclusion and Discussion: It is possible to state that in the cases of Traumatic Embolism of Air widespread pathology happens which affects all organs. It is possible to state OR13 that in these cases, the accumulation of gas is in all the Effect of hyperbaric oxygen treatment on traumatic tissues of the organism, but in the moment of shock, brain injury rat by magnetic resonance imaging moreover there are organs in charge of managing the Xiang Huang 1, Chunjing Gao 2 crisis which also suffer the effects of the embolism. 1. Bejing Shunyi Hospital of China Medical University, Beijing, China O12 2. Beijing Chaoyang Hospital of Capital University of 24 cases of neurological decompression illness - 14 Medical Science, Beijing, China months of a single center experience 1 1 2 Jorge Calderón , Cristian Melián , andrés Reccius Objective ：：：To investgate dynamic changes of the effect of 1. Underwater Medicine Service, Hospital Ancud hyperbaric oxygen treatment(HBOT) on traumatic brain (UMSHA), Ancud, Chile injury (TBI) during the initial 7 days by using lateral 2. Pontificia Universidad Católica, Chile fluid-percussion (LFP) rat modal and magnetic resonance

Objectives: To review the first 14 months of operation of imaging (MRI). Meterials and Metheods ：：： 30 male the Underwater Medicine Service of the Hospital de Sprague-Dawley rats were randomly divided into 4 groups: Ancud, Chile (UMSHA). To describe the cases of treated HBOT group(n=10), TBI group(n=10),1st day TBI neurological decompression illness (NDCI). Methods: group(n=6), sham control group(n=4). MRI was performed Prospective systematic registry of the conditions with a at 1 day, 3 day, 5 day, 7 day after severe injury on lateral detailed profile of diving and neurological examinations fluid-percussion. HBOT was performed to rats in HBOT made by a neurologist specialized in diving medicine. group each day after 24 hours. The rat brain imaging was Revision and analysis of these registries. Results: We evaluated according to a standard by 2 radiologist who review 60 cases of decompression illness treated from didn’t know groups divided. All rats were sacrificed March 2007 to May 2008, 24 (40%) of them had NDCI. Of immediately after MRI exam; their brains were removed these, 13 had involvement of the spinal cord, four of the and prepared for pathologic exam. Results ：There is no brain, and 7 had peripheral nerve disease. All patients difference between HBO and TBI group at 1 st day were treated with 6 USN, generally with two prolongations according to MRI evaluation. The difference tendency to 18 meters. Most cases with spinal disease had present at 3 rd day and 5 th day suggesting better paraparesia with a sensitive level and generally without improvement in HBOT than TBI group. The difference compromise of propiocepcion. Nine patients didn’t have th anterior spinal cord involvement, one patient had a becomes significant at 7 day. Conclusions：HBOT is complete spinal cord syndrome and one had a syndrome effective in accelerating brain tissue repair, promoting the that couldn’t be systematized. There were 2 cases that lesion clearance and hematoma or edema’s absorbing by couldn’t be classified in any subtype. Of these cases, four dynamic investigation of MRI. HBOT is also protective to brain tissue after the building of TBI rat modal for 24 hours which second brain damage have happened, the process is quantitative change to qualitative change. OR16 A survey on HBO treatment of traumatic brain injury this century in China—A important evidence of HBO OR14 improve curative effect and prognosis of Brain injury The effect of hyperbaric oxygenation on diabetic foot Zhou Shurong ulcers (the HODFU-study). A single centre, First Affiliated Hospital of Nanjing Medical University, prospective, randomised, double-blind Nanjing, China placebo-controlled parallel-group study Christer Hammarlund 1, Magnus Londahl 2, Per This report summarizes 214 articles about HBO treatment Katzman 2, Jan Apelqvist 3 of brain injury published on the proceedings of the 7 1. Helsingborg Hospital, Sweden national HBO academic congresses since this century. 2. Lund University Hospital, Sweden According to the statistics, 28878 cases of brain injury and 3. Malmoe University Hospital, Sweden 2088 case of PVS have been treated with HBO in this period. Among the 28878 cases of brain injury, 67.5% ～ Objective: To evaluate effects of hyperbaric oxygenation 88.2% were cured or notably effective, the total effective (HBO) on long-term ulcer healing in patients with diabetes mellitus and chronic foot ulcers where further possibilities rate was 89.3% ～99.6%. While in the 2088 cases of PVS, for vascular surgery has been ruled out. Methods: 94 46.7% ～58.83% were cured or basically cured, the total Patients from three hospitals in southern Sweden were enrolled in the study. Randomisation was stratified for effective rate was 82.25% ～97%. In this report 12 articles toe-pressure with a cut-off at equal or less than 30 mm Hg. were cited, the RCT and Meta-analysis of which were Both HBO and hyperbaric air (HBA) treatment was given compared with the data from some brain injure therapy at the same time in the chamber in a double blind fashion. research centers in the world. The results show that HBO The patients received a minimum of 37 and a maximum of synthesize therapy is better than the routine treatment in 40 treatments at 2.5 ATA and a duration of 90 minutes. the improvement of curative effect and prognosis. This Trancutaneous oxymetri was used for evaluating the report presente ample evidences for HBO sythesize effects of HBO/HBA-treatment. In this presentation are no therapy in raising effective and improving prognosis. subgroups analysed. Results: The median time to ulcer healing was 5 months in the HBO-treated group and 12 months in the HBA-group. A blinded observer did this evaluation. Approximately half of the wounds did not heal. Trancutaneous oxymetri showed successive higher OR17 oxygen levels on the forefoot during the chamber treatment in the HBO group compared with no rise in Clinical analyses of 429 Cases of Acute CO Poisoning Zhuo Li 1, Chunjin Gao 1, Xiang Huang 2, Huan Ge 1, Yu Ga oxygen levels in the HBA group. The differences between 1 the two treatment groups lasted at least 1½ year. o Conclusion: Hyperbaric oxygenation is a valuable 1. Beijing Chaoyang Hospital, Beijing, China adjunctive when treating diabetic foot ulcers. 2. Beijing Shunyi Hospital, Beijing, China

Introduction: Acute CO poisoning frequently occurs in winter in northern part of China. After CO poisoning, COHb has an evident increase. It is well known that rising OR15 COHb affects the transportation of oxygen and triggers Hyperbaric Oxygen Therapy for 16 Gas Gangrene the poisoning. Methemoglobin formation results from the Patients Injured by Earthquake oxidization of the bivalent iron of hemoglobin into trivalent Pan Fu-Qiong, Zeng Yu, Nie Cai-Xian, Huang Ling-Ling, iron, which lacks the capacity of carrying and releasing Tang Ming-Chan, Li Yu-Feng, Zhang Yun oxygen. However, there is no literature reporting the Sichuan Provincial Hospital, Chengdu, China change of Methemoglobin formation in acute CO poisoning patents (ACOP). With the application of Rad-57 Objective: observe the therapeutical effect of Hyperbaric Pulse CO-Oximeter, (Masimo Corporation) we have oxygen for 16 gas gangrene patients injured by observed the changes of COHb and MetHb of 429 acute earthquake. Methods: establish emergency isolation CO poisoning patients and studied the roles of COHb and passage, rescue each case with hyperbaric oxygen at the MetHb in triggering acute CO poisoning as well as their pressure of 0.25Mpa,7 times per 3 days,20 times totally. relations. Methods: The subjects are 429 acute CO And psychological guidance is added. Results: all poisoning patients in Beijing Chaoyang Hospital and wounds recovered in 16cases,including 4 cases with their Beijing Shunyi Hospital from November, 2006 to March, unamputated limbs preserved. Fusiform bacillus can not 2007 with 100 healthy people who do not smoke as the be detected. Conclusions Hyperbaric Oxygen can control group. We employed Rad-57 Pulse CO-Oximeter effectively curb the growth of Fusiform bacillus, decrease to observe the levels of COHb and MetHb of the the production of toxin, promote the heal of wounds, lower emergency patients when they went to see the doctor. the disability rate and raise the survival rate. After 2h inhaling of oxygen (through nose at the rate of 3L/min) or one hyperbaric oxygen theraphy (2ATA, the same time, multicentre unite research should be breathe oxygen for 60min), the levels of COHb and carried out for better therapeutic measures. MetHb were measured again and the changes observed. We also recorded general situation, cause of the disease and clinical classifications etc. of the patients. Then we utilized SPSS11.0 software for statistic analysis. Results: OR19 CO poisoning of 98% of the patients is due to Effect of HBO on endogenous neural stem cells in rat inappropriate use of coal stoves. Clinical classification: models of acute CO poisoning Light poisoning 274 cases; intermediate poisoning: 77 Wenlan Wang, Jinsheng Li, Xiaoping Xie cases; severe poisoning: 78 cases. A total of 98 Department of hyperbaric oxygen treatment center, emergency patients have MetHb>1.2% at the first School of Aerospace Medicine, Fourth Military Medical measurement. The concentrations of both COHb and University, Xi’ an, China MetHb of the patients are evidently higher than that of the control group (p0.05) although fifty patients out of 58 Objective: To discuss the effect of HBO on endogenous cases showed small decrease in MetHb. Discussion and neural stem cells (NSCs) in rat models of accute CO conclusions: (1) Inappropriate use of coal stove is the poisoning, and to analyses the mechanism of HBO main cause of acute CO poisoning in northern part of treating brain injury after accurate CO poisoning. China. (2) MetHb may be involved in the Methods: After set up models of accurate CO poisoning, physiopathological process of hypoxia of ACOP patients. H-E staining was used to observe the pathological MetHb of such patients is clearly higher than that of the changes of brain tissues and immunohistochemical control group, but only 98 patients have MetHb >1.2%. staining was used to observe the expression of nestin This indicates that the rise of MetHb level is not the key (NSCs specified marker) and GFAP (astrocyte specified factor leading to hypoxia in ACOP patients. The increase marker). Results: The H-E staining showed that the of COHb level is the main cause. (3) Whether hyperbaric morphology of neurons in control group were normal, and oxygen treatment can effectively cure which of poisoned groups were degenerated and necrotic methemoglobinemia still requires further study. (4) The in spots and sheets, and the cortex of CO poisoned Rad-57 Pulse CO-Oximeter provides a noninvasive tool to groups were looser as well as the cone cells of greatly facilitate the clinical diagnosis & treatment for CO hippocampus were thinner, but in HBO group the poisoning patients. degenerated and necrotic neurons became less. The Table 1 immunohistochemical staining showed the following: group COHb (Mean%) MetHb (Mean%) expression of nestin and GFAP was normal in quantity Control group 1.28±0.68 0.25±0.12 and morphology in control group, in CO poisoned groups ACOP group 18.28±8.40 0.86±0.82 the expression of nestin was increased lightly and the expression of GFAP was abnormal in quantity and P -value <0.01 <0.01 morphology, in HBO group GFAP positive cells became normal, but the expression of nestin was increased obviously. There are nestin/GFAP double positive cells in cortex where the damage of neurons was extremely heavy. Conclusion: Compared to control group, the OR18 quantity of nestin + cells was increased lightly, astrocytes Review of Current situation on acute carbon were proliferated and deformed and neurons get monoxide poisoning in china degenerated and necrotic in poisoned groups and HBO Shuyi Pan, Xiaowen Pan, Yu Zhang, Yan Lu, Xiangen Me could make astrocytes become normal and have NSCs ng, Chen Yang, Mingxin Li, Qi Zhang, Liang Zhang generate, immigrate and differentiate. It is suggested that Navy General Hospital, Beijing, China the process of brain injury can make NSCs active and HBO can treat the brain injury by generation, immigration To summarize the study on acute carbon monoxide and differentiation of NSCs. poisoning (ACMP) of Chinese Units in recent 10 years, including pathogenesy, clinic treatment and animal model building. And analyze the existing problems and reasons of ACMP. The conclusion that morbility foundation of OR20 ACMP and delayed encephalopathy may be poisoning Hyperbaric oxygen treatment attenuated the decrease cascade reactions in which multiple pathomechanisms in regional glucose metabolism of rats subjected to play roles; It maybe one of important factor that patient focal cerebral ischemia: a high resolution positron breathed in concentration of CO for prognosis. The key to emission tomography study resolve this problem is early intervention. Reviving the M. LOU 1, H. ZHANG 2, J. WANG 3, S. Q. WEN 1, Z. Q. respiration and circulation early to ensure utility filling into TANG 4, Y. Z. CHEN 5, W. Q YAN 6, M. P. DING 1 tissues and organs, sustaining cell normal metabolism, 1. Department of Neurology, the Second Affiliated especially to brain tissue. The treatments are including, Hospital, Zhejiang University, School of Medicine, active HBO treatment, keeping breathing smooth, Hangzhou, China ensuring utility oxygenation and circulation, improving 2. Department of Nuclear Medicine, the Second Affiliated microcirculation, anticoagulation, reducing plasma Hospital, Zhejiang University, School of Medicine, fibronectin. In addition, cytoprotection measures such as Hangzhou, China anti-free radicle should be given at the right moment. At 3. Zhejiang-California International Nanosystems Institute, drilling cranium II group (28cases, the volume of Hangzhou, China hematoma ≥50ml), micro-found drilling cranium+ HBO 4. Department of Hyperbaric Oxygen, the Second group (36cases, the volume of hematoma ≥50ml); their Affiliated Hospital, Zhejiang University, School of curative effects, complications, the hospitalization time Naformátováno: Písmo: Medicine, Hangzhou, China and hospitalization cost were compared respectively. (výchozí) Arial, 9 b., Tučné 5. Department of Neurobiology, Zhejiang University, Before and after the HBO treatment, the haemodynamics School of Medicine, Hangzhou, China parameters of HBO group were detected and compared. Naformátováno: Písmo: 6. Clinical Research Center, the Second Affiliated Results: The 94cases got an excellent prognosis. The (výchozí) Arial, 9 b., Tučné Hospital, Zhejiang University, School of Medicine, sequela, hospitalization time and cost in micro-found Naformátováno: Zarovnat Hangzhou, China drilling cranium I group were lower than in conservative group (P<0.05); and in micro-found drilling cranium+ HBO do bloku Cerebral hypoxia may be the main component of cell group were lower than in micro-found drilling cranium II Odstraněno: Trial of damage caused by ischemia. Previous studies group (P<0.05). After the HBO treatment, the cabin-oxygen-concentrati demonstrated a neuroprotective effect of early hyperbaric haemodynamics parameters of patients were ameliorated, on distribution of oxygen (HBO) treatment in various animal models of focal and the difference were significant when compared with cerebral ischemia. Neuropathologic study showed that the before (P<0.05). Conclusion: The treatment of the Odstraněno: single oxygen exposure of HBO may prevent cell death in ischemic micro-found drilling cranium combined with the HBO on pressurized module cortex. In the present study, we aimed to assess cellular patients with extradural hematoma can significantly function of ischemic rat brain after HBO treatment by increase the cure rate, decrease mutilation rate, and Naformátováno: Písmo: means of a high-resolution positron emission tomography effectively shorten the hospitalization time, save treatment (výchozí) Arial, 9 b., není scanner (microPET) used specifically for small animal cost. Tučné imaging. The male Sprague– Dawley rats were subjected to permanent middle cerebral artery occlusion (MCAO), Naformátováno: Písmo: with the regional cerebral blood flow monitored in vivo by (výchozí) Arial, 9 b., není laser Doppler flowmetry. One hour after ischemia, HBO OR22 Tučné therapy (3 atm absolute, 1 h) was initiated. Local cerebral The effect of hyperbaric oxygen on the osteoporosis glucose utilization in the ischemic area was measured rats Naformátováno: Písmo: before, 1 h and 3 h after ischemia, with Wang Peisong, Wan Jin’e, Yang Xizhong, Wang Shuzhen, (výchozí) Arial, 9 b., není 2-[18F]-fluoro-2-deoxy-d-glucose (FDG) as a tracer. Liu Xueling, Lu Qiuning Tučné Neurological deficits and infarct volumes were assessed Department of hyperbaric oxygenation, The affiliated at 24 h after ischemia. Our study showed that early HBO Hospital of Qingdao University Medical College, Qingdao, Naformátováno: Zarovnat therapy significantly reduced infarct volume of brain 24 h China do bloku, Řádkování: after ischemia. Moreover, glucose utilization in the jednoduché ischemic area underwent asevere decrease during 1–3 h Objective: To inverstigate the impact of hyperbaric after MCAO, while the early HBO treatment significantly oxygen on bone mineral density and bone biomechanical Naformátováno: Písmo: attenuated the decrease in cerebral metabolic rate of property . Methods: Reproduce the osteoporosis model (výchozí) Arial, 9 b., není glucose in the ischemic core of the cortex compared with of ovariectomized rats and received hyperbaric Tučné controls. We report for the first time the application of oxygen and lycopene therapy. After 12 weeks microPET to quantify the rates of glucose metabolism in therapy,bone mineral density and bone biomechanical Naformátováno: Písmo: the ischemic core of rats exposed to HBO. Our results property were measured. Results: The bone mineral (výchozí) Arial, 9 b., není suggest that the early exposure of HBO can partially density and biomechanical property of ovariectomized Tučné reverse the downward trend for glucose utilization in the rats decreased significantly (P<0.05), and it increased ischemic core, which might contribute to the reported after 12 weeks therapy using hyperbaric oxygen and Naformátováno: Písmo: beneficial effects of early HBO therapy on permanent lycopene, and there were no significantly between (výchozí) Arial, 9 b., není cerebral ischemia. hyperbaric oxygen and sham groups. Conclusions: Tučné Hyperbaric oxygen and lycopene could improve and increase the bone mineral density and bone Naformátováno: Písmo: biomechanical property in (výchozí) Arial, 9 b., není OR21 ovariectomized rats; Hyperbaric oxygen and Tučné Treatment of micro-found drilling cranium and lycopene have the nearby effect . hyperbaric oxygen on 94 cases of patients with Naformátováno: Písmo: extradural hematoma (výchozí) Arial, 9 b., není Jincheng CHENG, Yiqun WANG, Bensong YU, Ganf SU, Tučné Shiquan YANG, Tian XIA OR23 123th Hospital of People’s Liberation Army Research on the distribution of oxygen concentration Naformátováno: Písmo: in the Sinlge Hyperbaric Oxygen Chamber 1 1 1 (výchozí) Arial, 9 b., není Objective: To evaluate the curative effects of micro-found Qibiao Weng , Hongjuan Wang , Lingzhen Chen , Tučné drilling cranium and hyperbaric oxygen (HBO) for patients XuelinChen 1, Zhenxin Huang 2, Huai Huang 3, Xiangxue Li 4, 4 4 with extradural hematoma. Methods: According to the Guirong Lin , FengxianWei Naformátováno: Písmo: volume of hematoma and GCS points of patients, ninety 1.Department of Hyperbaric oxygen, Zhujiang Hospital, (výchozí) Arial, 9 b., není four cases of extradural hematoma were divided into four the Second Affiliated Hospital of Southern medical Tučné groups: conservative group (14cases, the volume of university, Guangdong province, China. hematoma<50ml), micro-found drilling cranium I group Naformátováno: Zarovnat (16cases, the volume of hematoma<50ml), micro-found do bloku 2.Department of Hyperbaric oxygen, the Second Affiliated We found that the concentrations were same everywhere Hospital of Guangzhou medical college, Guangdong in the cabin while the pressure was at 0.1MPa lasting for Odstraněno: Huang Zhenxin 1, Weng Qibiao 2, province, China. 40min. Nevertheless, great difference took place while 2 3.Department of Hyperbaric oxygen, Guangzhou General pressure reducing; concentration on bottom was higher Wang Hongjun , Chen 2 2 Hospital of Guangzhou Military Area Command of than that in other parts. The concentration came to Lingling , Chen Xuelin , Huang Huai 3, Li Xiangxue 4, Chinese PLA, Guangdong province, China. coordinate again when pressure dropped to 0.03MPa. 4 4 4.Department of Hyperbaric oxygen, the Hospital of Suggestion 1.The location of sampling spots should be Lin Guirong , Wei Fengxian ¶ Guangdong Water Resources and Hydropower standardized.2.The location of oxygen inlet/outlet should 1. Zhujiang Hospital engineering Bureau 2, Guangzhou, China. be standardized. Oxygen inlet should be set at the top of attached to Southern the posterior part of the chamber, while oxygen outlet Medical University, Department of Hyperbaric Objective ：：： This study was designed to explore the should be set at the bottom of the anterior part of the chamber.3.The way to wash cabin should be applied oxygen, Guangzhou, China¶ distribution of oxygen concentration in different parts of according to the need of the patient so as to achieve the 2. The Second Hospital the hyperbaric oxygen chamber and the possible appropriate oxygen concentration.4.The production of the attached to Guangzhou influence of the different locations of oxygen inlet/outlet stretcher should be standardized. First, it must be Medical College, and the methods of washing cabin. The oxygen inlet/outlet equipped with a mattress. Second, enough space should Department of Hyperbaric were located in different ways and the oxygen samples be vacated beside the stretcher in the chamber.5. A new oxygen, Guangzhou, China¶ were gathered in the upper, middle and lower parts of the function should be developed in order to mix the gas 3. General Hospital of chamber in our test. The aim of our study was to select automatically and make help to the gas diffusion in the Guangzhou military district, the most appropriate location of oxygen inlet/outlet and hyperbaric oxygen chamber. Department of Hyperbaric the most appropriate method to wash cabin so as to oxygen, Guangzhou, China¶ enhance the uniformity and concentration in the chamber, 4. Department of Hyperbaric which could improve the theraputical efficiency of oxygen, Hospital of Second hyperbaric oxygen. Materials and methods ：：：We used 7 Water and Electricity Office OR24 choices of combination of different ways letting in or of Guangdong Province, Clinical Research on Effect of HBO plus Electric letting out the oxygen. Meanwhile, whether patient was in Guangzhou, China¶ Stimulation for Treatment the Cerebral Resuscitation chamber and if the continuous washing was executed had Peidong Wang, Chunping Wu, Hulong Ma, Kangmin Xie, Naformátováno: Zarovnat been concerned. Finally, 15 methods came out under Yaojun Zhang, Xia Wang, Aiping Wang manifold of situation. Result and Discussion: Three do bloku Chinese HBO Medicine Association Research Center of essential elements: the ways of oxygen inlet/outlet, the Brain Recovery, Nanjing, China Naformátováno: Písmo: state of cabin, the ways of washing, was aimed in our trial Tučné for each of them takes great changes to the elevation of To improve therapeutic effect on cerebral brain oxygen concentration and distributive uniformity. Three resuscitation, a comprehensive therapy with hyperbaric Odstraněno: The medical positions of inlet/outlet, three states and three opinions of oxygen (HBO) treatment as the main method was oxygen pressurized cabin washing cabin was developed in raising, stable and adopted from 1991 April to 2006 Dec. Among total 973 which is convenient and decreasing pressure period with 9 sampling spots set on cases of PVS patients; 14 cases patients were put to use suitable for patients with the chamber. The trial was executed with the pressure electrical stimulation (ES) demonstrated more excellent different diseases and ages under 0.1MPa, lasting for 80min and strictly following the effect. Judgment of therapeutic efficacy: - Rely on PVS has been widely used for rule of Chinese medical association-HBO branch. scoring scale and criteria of therapeutic efficacy (Nanjing, decades. Meanwhile, it is Measurement range is 0-100% with sampling interval of 2001). Basic recovery (consciousness or cognition accepted by a majority of 2min. 1.Influential factors on the distribution of recovery) 371cases (38.13%); Obvious improvement hospitals. To select oxygen concentration. The main factors can be the size (score ≥ 8 –12 points) 203 cases (20.86%); Obvious appropriate input/output of of stretcher and the location of oxygen inlet/outlet. improvement (score ≥ 3 points) 190cases (19.5%); No oxygen, structure and Unsymmetrical distribution was found, causing by the gas improvement (no change, worsening or death) 209 cases; washing method is shot circuit on the bottom of wide size stretcher, which The general improvement rate is 78.48%; The HBO plus beneficial to oxygen was widely used in single hyperbaric oxygen chamber. In ES is superior to the results of similar HBO treatment. concentration elevation and contrarily, the oxygen inlet on the top or middle side Some causes that affect efficacy in treatment of PVS are distribution in cabin, braces the concentration to 65-75% with the symmetrical analyzed as follows: From the result, it can be seen that because pure oxygen is distribution. On the other hand, the modification on the efficacy of HBO treatment in traumatic cases is used as medium for stretcher make help to the gas diffusion in the chamber. obviously higher than that of non—traumatic cases; the compression. Three ways of 2.The best method of washing cabin. Only younger the onset happens, the higher the curative rate is; oxygen input/output(on the wash-procedure with the pressure-raise to 0.1MPa the earlier HBO treatment is used, the better the efficacy top, middle and bottom of showed distinctive difference of 2% elevation at the top is; and HBO plus ES, if available in some indication cases, the cabin), three states of and 4% at the middle and bottom parts. Moreover, the shows that the more excellent effect. We suggest that the cabin(with a bed, with a normal pressure method can be the best for it raise the PVS patients should receive HBO therapy (and certain bed and a patient, without a concentration effectively, especially up to 80%in 5 cases plus ES) as early as possible is the best choice bed and a patient) and three minutes in the middle part. 3.selection of sampling ways of washing spots Since the different design of stretcher and the method(continued washing inlet/outlet locations vary, the regular way to set the spots method with boosting on the top of stretcher and middle of chamber fails reflect pressure, washing method the distribution of concentration. However, changing the of boosting pressure of... the [29] inlet position and taking the appropriate way washing OR25 cabin can provide consistent samples everywhere in the Naformátováno: Angličtina chamber. 4.the distribution of oxygen concentration (USA) Effect of Hyperbaric Oxygen Treatment on the content Wang Youbin 1, , Gao Chunjin 2, Liu Fujia 2, Sun Xuejun 3, Liu of VEGF, HIF-1,CX43 in random skin flaps of rabbits Xuehua 2(Qi Zheng and Gao Chunjin contribute equally in Xuehua Liu 1, Chunjin Gao 1, Huan Ge 1, Fujia Liu 1, Yang this experiment) Liu 2, Hongying Zhao 2 1. Plastic Surgery Department of Peking Union Medical 1. Department of Hyperbaric Oxygen, Chaoyang Hospital, College Hospital, Beijing, China. Capital Medical University 2. Hyperbaric oxygenic Deperpartment of Beijing 2. Department of pathology, Chaoyang Hospital, Chaoyang Hospital associated with Capital Medical Capital Medical University University, Peking, China. 3. Department of Chinese Military Medical University, Shanghai, Chnia. Purpose: To observe the changes of capillary density, vascular endothelial growth factor(VEGF), Objective: To study the interfering effect of hypoxia-inducible factor-1(HIF-1), Connexin preconditioning hyperbaric oxygenation on skin flap 43(CX43)before and after hyperbaric oxygen treatment in ischaemia tolerance. Methods : 18 SD rats were divided the transplanted random skin flaps of rabbits and to the control group and the HBO preconditioning group. In explore the mechanism of capillary ingrowth of control group, an extended epigastric adipocutaneous flap hyperbaric oxygen treatment on random skin flaps of was raised, based on the right superficial epigastric artery rabbits. Materials and Methods: 12 rabbits were and vein. 3-hours flap ischemia was induced by clamping randomly divided into two different groups: control the pedicle vessels with microvascular clamp. At the end group(n=6), hyperbaric oxygen group(n=6). Random skin of ischemia induction, the clamp was removed and the flaps models were established on the back of rabbits. The flap was sutured back. Rats in HBO preconditioning group control group was placed in normal pressure atmosphere were treated with HBO two days before operation. Flap without hyperbaric oxygen treatment. The hyperbaric surgery began 1 hour after the last HBO treatment. The oxygen group started to accept hyperbaric oxygen operation was the same as the control group. The size of treatment(2 ATA, 45min per time) after flap the designed flap and the survived flap on the fifth transplantation immediately, twice a day from postoperative day was duplicated with transparent paper. post-operation to the third day, then reduced to one time The condition of the flap was recorded with digital camera. per day to the seventh day, seven days altogether. we , The picture of the paper was gotten with scanner and it s observed the survival area of random skin flaps and area which was equal to the area of the flap was detected capillary density and content of VEGF, HIF-1, calculated with Acrobat soft ware. Data were analyzed CX43 by using HE staining and immunohistochemistry at rd th with SPSS soft ware. Results : The average designed 3 day and 7 day respectively. The SPSS11.5 software 2 flap areas were 51.59 and 52.71 cm in the control group was used for statistical analysis. Results: 1.The survival and the HBO preconditioning group. The average survival area of skin flaps in the hyperbaric oxygen group was 2 flap areas were 7.38 and 15.82 cm . There were obvious singnificantly higher than that of the control group at 3 rd difference between the control and the HBO day and 7 th day (p<0.01); 2.The capillary density in the preconditioning group (t=4.14, P=0.003) in average hyperbaric oxygen group was higher than that of the survival area. Conclusion : HBO preconditioning can rise control group at 3 rd day (p<0.05),the capillary density in flap ischaemia tolerance and enhance flap survival. hyperbaric oxygen group was singnificantly higher than that of the control group at 7 th day (p<0.01); 3.The content of VEGF in the hyperbaric oxygen group was higher than that of the control group at 3 rd day and 7 th day (p<0.05); 4. OR27 The content of HIF-1 in the hyperbaric oxygen group was Effect of early hyperbaric oxygen treatment on lower than that of the control group at 3 rd day and 7 th day prevention of disorder of coagulation-fibrinolysis (p<0.05); 5.The content of CX43 in the hyperbaric oxygen systems in rabbits with steroid-induced avascular group was singnificantly higher than that of the control rd necrosis of the femoral head group at 3 day(p<0.01),the content of CX43 in the 1 2 3 WANG Yong , GAO ChunJin , PANG BaoSen , YANG hyperbaric oxygen group was higher than that of the 4 1 2 th JinCai , LI HaiDong , WU LianHua control group at 7 day (p<0.05). Conclusions: 1. Department of Hyperbaric oxygen, Fuxing Hospital, Hyperbaric oxygen treatment can accelerate capillary Capital Medical University, Beijing, China ingrowth by increasing the expression of VEGF and CX43, 2. Department of Hyperbaric oxygen, Beijing Chaoyang so improve the survival rate of transplanted random skin Hospital, Capital Medical University, Beijing, China flaps. Hyperbaric oxygen treatment can improve the 3. Department of Respiratory Diseases, Beijing Chaoyang hypoxia state of transplanted random skin flaps and Hospital, Capital Medical University, Beijing, China decrease the expression of HIF-1 in transplanted random 4. Department of Orthopaedics, Beijing Chaoyang skin flaps. Hospital, Capital Medical University, Beijing, China

Objective: To establish animal model of steroid-induced

OR26 avascular osteonecrosis of the femoral head (SANFH)； Hyperbaric Oxygenation Preconditioning Induce Skin To investigate the effects of hyperbaric oxygen (HBO) Flap Ischaemia Tolerance in Rat Model therapy on histopathology, coagulation and fibrinolysis of SANFH. Methods: 1. Experimental animal and group: Male adult New Zealand white rabbits, ranging in weight from 2.5 to 3.5 kg, were obtained from experimental hours, 1 and 2weeks(P<0.01), then began to decrease center of capital university of medical sciences and after 2 weeks, the value were not different than group N at randomly divided into eleven groups including Group N 4 and 6 weeks. group H were significantly lower than (control group); Group M 24h , M 48h , M 1, M 2, M 4, M 6(model group M at 2 weeks(P<0.01), whereas not different than groups); GroupH 1, H 2, H 4, H 6(HBO groups). Each group group N. Conclusion: HBO intervention can inhibit had eight rabbits. 2. Model establishment and HBO hyperplasia and hypertrophy of fat cells in marrow, reduce therapy: According to Yamamoto T et al’s method, the extent of osteonecrosis and enhance the ability of animals in group M and H reveived lipopolysaccharide fibrous reparation and bone reconstruction after (LPS) 20 g/kg intravenously 2 times at an interval of 24 osteonecrosis; HBO therapy can improve obstruction of hours, injected methylprednisolone 20mg/kg coagulation and fibrinolysis of SANFH. intramuscularly into the gluteus medius 3 times at intervals of 24 hours after the second injection of LPS. Animals in Group H 1, H 2, H 4, H 6 were received HBO therapy after the second injection of LPS, 2.0 ATA, 1 hour daily, once a day. Group H 1, H 2, H 4 , H 6 were treated 1, OR28 2 ,4 weeks and 5 weeks respectively. 3. Experiment Hyperbaric oxygen preconditioning protects against parameters: Antithrombin-III (AT-III) 、 traumatic brain injury at high altitude Shengli Hu, Rong Hu, Fei Li, Yongzhi Xia, Hui Meng, Gao Thrombomodulin(TM) 、 Tissue-type plasminogen yu Cui, Hua Feng activator(tPA) and Plasminogen activator Department of Neurosurgery, Southwest Hospital of the inhibitor-1(PAI-1 ) were measured in each group; Animals Third Military Medical University, Chongqing, China in group M and H were killed at 2 weeks, 4 weeks and 6 weeks respectively after the second injection of LPS and Background Recent studies have demonstrated that the tissue samples of the femoral head were observed preconditioning with hyperbaric oxygen (HBO) can reduce with light microscopic examination. Results: 1. ischemic and hemorrhagic brain injury. The aim of this Histopathologic examination: In group N, the bone study was to investigate the effects of HBO trabeculaes align trimly in subchondral zone of femoral preconditioning on traumatic brain injury (TBI) at high head. Empty lacunae were found occasionally. Large altitude and examine the role of matrix hematopoietic tissues were seen in marrow with fat cells metalloproteinase-9 (MMP-9) in such protection. Methods disseminated sporadically. In group M, it was Thirty-nine male Sprague-Dawley (SD) rats were distinguished that fat cells were increased in both the randomly divided into three groups: HBO preconditioning number and the size, whereas hematopoietic tissues were group (HBOP n=13), high altitude group (HA n=13) and decreased; Subchondral bone and bone trabeculae high altitude sham operation group (HASO n=13). All showed empty lacunae, pycnotic nuclei and apparently groups were subjected to head trauma using a weight discrete; Among trabeculae, fat cells showed hyperplasia drop device except for the HASO group. The rats in the and hypertrophy, such pathologic changes showed HBOP group received 5 sessions of HBO preconditioning particularly significant in group M 4, in which marrow were (2.5 ATA, 100% oxygen, 1 hour daily) and then were kept nearly accumulated with fat cells and the disruption and in a hypobaric chamber at a pressure of 0.6 ATA(simulate fusion of fat cells were seen obviously. In group H, the the pressure of high altitude of 4000 meters) for 3 days number of fat cells was more than that in group N, less before operation. The animals in the HA group received than that in group M, obviously changes were found in control pretreatment (1 ATA, room air, 1 hour daily), then group H4 and H6; Apparently trabeculae loose were not followed the same procedures to the HBOP group. In the found; pycnotic nuclei and empty lacunae rate were HASO group, rats were only opened a skull window significantly decreased than those of group M. Fibrous without injuring the brain. Twenty-four hours after TBI, tissue becomes more prominent in marrow in group H 6 neurological function and brain water content of 7 rats in and there were apparently active osteoblasts surrounding each group were examined and 6 rats in each group were bone trabeculae in metaphyseal area. 2. Hematologic killed for hematoxylin-eosin staining and examination: AT-III : Compare to group N, group M were immunohistochemical analyses. Results The significantly higher at 24 hours after the first injection of neurological outcome in the HBO group (0.71±0.49) was LPS(P<0.05), began to decrease at 48 hours, and better than that in the HA group (1.57±0.53; PP <0.01). reached minimum at 1 week (P<0.05), Group H were not The brain morphology and structure presented by light different in comparison with group N, whereas obviously microscope was destroyed greatly in the HA group, decreasing at 4 and 6 weeks(P<0.01); TM: Compare to including massive cells necrosis, conspicuous edema of group N, group M began to increase at 24 hours after interstitium, hemorrhage, and so on, while less the first injection of LPS, and reached maximum at 1 pathological injuries occurred in the HBO group. week(P<0.01), group H were significantly lower than Compared to HA group, pretreatment with HBO group M at 1week(P<0.05), whereas more higher at 4 significantly reduced the number of MMP-9-positive cells weeks(P<0.05); tPA: Compare to group N, group M began (92.25±8.85 vs. 74.42±6.27; P<0.01). Conclusions HBO to decrease at 24 hours after the first injection of LPS and preconditioning can attenuate TBI in rats at high altitude. reached minmum at 2 weeks(P<0.01), group H were The decline of MMP-9 expression may contribute to HBO significantly higher than group M at 2 weeks(P<0.05), preconditioning-induced protection of brain tissue against whereas not different than group N; PAI-1: Compare to TBI. group N, group M began to increase at 24 hours after the first injection of LPS and were significantly higher at 48 OR29 other therapy in accord with individual needs. The group A The effect of hyperbaric oxygen on the proliferation was randomly divided into two groups –group and death of Nasopharyngeal Carcinoma Cells and its A1(experimental group)(22) and A2 (observation mechanisms group)(21).And group A1 was in Hyperbaric Oxygenation Zhengrong Peng, Juan Liu, Weihong Zhong, Pingtian Xia (HBO) synchronously ,but group A2 not .HBO was 2 n hours per day and 6 times per week . The Modified Xiangya Hospital, Central South University, Changsha, Barthel Index (MBI) was used to assess patients' capacity China in B-ADL. Assessment was done upon admission to rehabilitation and 6 weeks afterwards. Results: Before Objective: To investigate the effect and the possible treatment, the patients in A1 and A2 scored significantly influencial mechanisms of hyperbaric oxygen(HBO) on lower with BI than those in B (P < 0. 05). After treatment, the proliferation and death in human nasopharyngeal the patients in all groups scored significantly higher with carcinoma(NPC) cell line CNE2Z by viewing the inhibition BI assessment (P < 0. 001). However, the score with BI ratio of proliferation(IROP) and mortality rate(MR) and the assessment in A2 was lower than those in B, but no content of superoxide dismutase(SOD) and significant difference (P >0. 05), and there was no malondialdehyde(MDA) of nasopharyngeal carcinoma significant difference between A1 and B (P > 0. 05). But cells through HBO disposal. Method: Nasopharyngeal there was significant difference between A1 and A2 (P< 0. carcinoma(NPC) cell line CNE2Z were divided into 3 05). Conclusion: USN had significant impact on the groups randomly, Group A: control group; Group B: recovery of the independent living of stroke patients. The hyperbaric oxygen(0.20MPa);Group C:hyperbaric rehabilitative intervention for USN may improve ADL oxygen(0.25MPa). The IROP in CNE2Z cells of all groups performance of stroke patients with USN. were detected by MTT reduction assay and the MR were detected by PI staining; as well as the content of SOD and MDA were detected. Result: The IROP and MR of B, C groups were dramatically increased comparing with A group(P0.05);There were no statistical difference in the content of SOD between A, B and C groups(P>0.05);There were statistical difference in the content of MDA between A 、B and C groups(P0.05). Conclusion: HBO disposal could increase the IROP and MR of human NPC cell line CNE2Z, and elevated the MDA content in nasopharyngeal carcinoma cells, it had hinted that HBO disposal could inhibit the proliferation of NPC cells and promoted the death of NPC cells by increasing the content of MDA.

OR30 Effect of hyperbaric oxygenation (HBO) on unilateral spatial neglect (USN) Liu Jinglong Heilongjiang Rehabilitation Hospital, Ha’erbin, China

Objective: To evaluate the effect of Hyperbaric Oxygenation (HBO) on unilateral spatial neglect (USN) and basic activities of daily living (B-ADL) after stroke. Methods: Sequentially access 93 stroke patients with hemiplegia admitted to the Heilongjiang Province Rehabilitation Hospital, according with the following: first stroke, no aphasia, no dementia, no cognitive disorder and serious system disease, were classified upon admission as having USN (group A; n = 43; 46.2% of the entire sample) or not having such disturbance (group B(control group ); n = 50; 53.8% of the sample). The age of all was between 35 and 75 ,and began to get rehabilitation within 4weeks .By ‘ cross-out’ test, ‘digit cancellation’ test, ‘line-bisection’ , clock drawing , ‘free hand drawing’ test, we examined the unilateral spatial neglect. When 3 of the 5 tests are abnormal or more, we thought the patient had unilateral spatial neglect. Both groups received standard rehabilitation treatment at most 6 weeks after stoke onset, including daily physiotherapy, occupational therapy, traditional Chinese medicine and

壁报交流 Poster Discussion P01 brain injury is more frequently occurred among vegetative Postoperative treatments with hyperbaric oxygen and state patients. A standardized nomenclature and management of critical complications in 181 patients diagnostic criteria are needed. The pathogenesis with severe brain injury remained unknown and its therapy is only focused on Xiaowen Pan, Shuyi Pan, Juan Meng, Yu Zhang, Xiangen symptomatic treatment. HBOT won’t be disturbed after the Meng, Yan Lu, Huijun Hu, Chen Yang, Qi Zhang, Mingxin symptoms are controlled. Li Navy General Hospital, Beijing, China

Objective: to summarize our experience on postoperative P03 management of patients of very severe brain injury with Experimental research on hyperbaric oxygen therapy combined therapy of hyperbaric oxygen(HBO). Methods: against traumatic brain injury retrospectively summarizing 181 postoperative patients of Guo-Hua Wang 1, Xiang-Gen Zhang 1, Yong-Cai Li 2, Yong very severe brain injury from January, 2001 to December, Wang 2, Zheng-Lin Jiang 1 2005, in our section, all patients were treated by HBO. 1. Nantong University, Jiangsu, China Results : of the 181 cases, 5 died (3%), 15 went into 2. First Hospital of Nantong, Jiangsu, China persistent vegetative state (8%), 112 cases were severely disabled (62%), 49 patients were mild disabled or Objective: To observe the therapeutic effect of hyperbaric completely recovered (27%). Conclusions: the prognosis oxygen (HBO) against traumatic brain injury (TBI) in rats, of VSBI could be significantly improved on condition that the time window of efficacy and optimal treatment times, early, safe, and efficient hyperbaric oxygen treatment and to investigate the underlying mechanisms of HBO for being performed, combined injuries and crucial TBI treatment. Methods: TBI was induced in rats with the complications being prevented and managed in time. modified Feeney’s method. For the sham-operated group, only a bone window was opened without traumatic injury. An animal hyperbaric oxygen chamber was used to carry out HBO treatment. Treatment pressure was maintained P02 at 3ATA of duration 60min with compression or Vegetative state and paroxysmal dysautonomia after decompression time of 6min, respectively. The chamber brain injury was kept ventilation with pure oxygen at a flow rate of Qiuyou Xie, Ronghao Yu, Yanbin He, Jin Gu 2L/min during compression and HBO exposure. The Hyperbaric Oxygen Medical Centre, Guangzhou General treatment groups received HBO therapy at 3h, 6h, 12h Hospital of Guangzhou Military Command, Guangzhou, and 24h, respectively, after TBI. In addition, at 24h after China TBI three groups of rats received HBO therapy for once, three, or five times, respectively. The neurological Objective: To analyze the clinical features, nomenclature, behavior score, brain water content and Nissl staining criteria for diagnosis, possible pathogenesis, were measured to identify the time window of efficacy and pharmacological management and hyperbaric optimal treatment times. The content of SOD and MDA, oxygenation therapy (HBOT) of vegetative state patients levels of inflammatory cytokines (TNF-α, IL-6, IL-1β and with paroxysmal dysautonomia after brain injury. Methods: IL-10) and cell apoptosis (TUNEL method and mRNA of The clinical presentations, auxiliary examinations, bax, bcl-2 and caspase-3) in the surrounding brain tissue treatment effect and prognosis were retrospectively of the primary injury were determined to uncover the analyzed for 19 vegetative state patients with paroxysmal underlying mechanisms of HBO therapy. Furthermore, the dysautonomia after brain injury. Results: Among 19 expression of the markers of glia cells (GFAP, Vimentin patients, There were 12 cases of severe traumatic brain and S-100) were detected with immunohistochemistry. injury, 1 case of cerebellar hemorrhage and received Results: In addition to the time point 24h after TBI, HBO evacution of hematoma, 1 case of heroin toxic treatment at other three time points 3h, 6h and 12h encephalopathy, 2 cases of severe carbon monoxide displayed significant therapeutic efficacy on TBI, with poisoning, 3 cases of hypoxic-ischemic encephalopathy decreases of the neurology deficit score, brain water after cardiopulmonary resuscitation (1 for electrical injury, content and the damage of brain tissue (P0.05). 1 for coronary angiography and coronary stent Compared with control group, HBO treatment at 6h after implantation and 1 for cardiac arrest due to anaesthetic TBI elevated the content of SOD and IL-10, reduced the accident). They had most of the symptoms such as level of MDA, TNF-α, IL-6 and IL-1β in traumatic brain agitation, hyperthemia, diaphoresis, tachypnea, tissue. HBO treatment also increased the expression of tachycardia, hypertension, dystonia. No epileptic wave bcl-2 mRNA with decrease of the ratio of bax/bcl-2, was found on their electroencephalogram (EEG). Latent reduced the bax and caspsase-3 mRNA and the TUNEL period of physiological waves was prolonged and positive cells. Moreover, the three markers of glial cells all amplitude was fallen down on brain auditory evoked showed a high expression in the surrounding tissue of the potential (BAEP) and somatosensory evoked potential primary traumatic cortex. Conclusion: HBO exerted a (SEP). Varied degree lesions were detected in the cortex, neuroprotective effect on the brain tissue after TBI, the subcortex, or brainstem by neuroimaging. Most frequently efficacy increased when HBO treatment was carried out at used drugs were dopamine agonist or antagonist, earlier time after TBI. Meanwhile the increase of treatment benzodiazepines and muscle relaxants. Once their times raised the therapeutic effect of HBO at 24h after TBI. symptoms were controlled, HBOT could be prescribed for This effect of HBO may be potentially mediated through them. Conclusions: Paroxysmal dysautonomia following following mechanisms: inhibiting the oxidative stress, reducing the release of inflammatory cytokines, and Objective: Studies suggest that after brain injury, suppressing the neuronal apoptosis. hyperbaric oxygen (HBO) is neuroprotective by stimulating cell proliferation. We examine whether HBO promotes neural stem cells (NSC) to proliferate and differentiate in neonatal hypoxic-ischemic brain damage (HIBD) P04 rats. Methods: Seven-day-old rat pups were subjected to HBO treat 22 cases drowning child unilateral carotid artery ligation followed by 2 hours of Mao Lixian hypoxia (8% O 2). HBO was administered 2 ATA, once Wenlin Hospital, Taizhou, China daily for 7 days within 3 hours after HIBD. The proliferating neural stem cells in the subventricular zone (SVZ) and Objective: TO study the tread plan and interfering effect dentate gyrus (DG) were dynamically examined by of HBO on drowning child. Methods: sum up our 5-bromo-2-deoxyuridine (BrdU)/nestin experiencement on treat total of 22 drowning child with immunofluorescence. Nestin protein was detected by HBO in 2000-2007. Use large-scale HBO: prepare Western blot analysis at various time points (from 6 hours medicine etc, keep airway unblocked. Results: 19 cases to 14 days) after HIBD. The migrating NSCs were (86percent) was recovered, 2 cases leftover sequelae of examined by BrdU/doublecortin (DCX) nervous system 1cases was dead. Conclusion: HBO immunofluorescence 7 and 14 days after HIBD. The may decrease and inhibit micro-thrombokinesis by to phenotype of the newborn cells was identified by increase microcirculation blood flow dynamic, to improve BrdU/ β-tubulin, BrdU/ glial fibrillary acidic protein (GFAP) the merphous and function of blood cell and endotbelial and BrdU/O 4 (oligodendrocyte marker) cell. Using HBO for drowning child is distinct improvement immunofluorescence. Myelin basic protein (MBP) was particularly. HBO may decrease the sequelae of nervous examined by immunohistochemistry and pathological system partianlarly. changes of the brain tissue were detected 28 days after HIBD. Results: In neonatal HI rats treated with HBO, the proliferation of endogenous NSC was observed in the SVZ and DG. Cell numbers peaked 7 days after HIBD and P05 proliferating NSC migrated to the cerebral cortex at 14 d HBO treatment of ACOP poisoning delayed after HIBD. Twenty-eight days after HIBD, an increase in encephalopathy 51 cases newly generated neurons, oligodendrocytes and MBP was Yang Jingfu observed in the HBO group compared to the untreated Peoples Hospital of Baodi District, Tianjin, China and HIBD rats. Conclusions: This study suggests that HBO treatment may promote neurogenesis of the Objective: To study the clinical, HBO treatment of ACOP endogenous NSC in neonatal HIBD rats, contributing to poisoning delayed encephal opathy. Methods: 51 cases repair of the injured brain. of patients .HBO treatment,the pressure of oxygen Acknowledgments Projects supported by National chamber for the 0.2-0.25mpa,Using the 6 personal small Natural Science Foundation of China (the Science Fund of air pressurized module of the XINYING oxygen chamber the Chinese Academy of Sciences), Grant 30672240. plant HangZhou. Each of the cabin is 60mins,one for a group of treatment, The number of treatment is 30-60(that is 3-6 courses). Results: The group of 51 cases cared in 32 cases, Markdely effective in 10 cases, better in 8 cases. P07 Invalid in 1 cases, the total efficiency was 98%. Hyperbaric oxygen preconditioning induces Conclusions: For severe ACOP patients, advanced age, mitochondrial phenotype to prevent early apoptosis accompanied with hypertension, hypercholesterolemia, after spinal cord ischemia in rats cerebral Infarction or other disease, longer time for coming Liping Wang 1, Xuejun Sun 2, Wenxian Li 1, Zhimin Kang 2, round and insufficient HBO treatment might be important Yun Liu 2, Hengyi Tao 2, Weigang Xu 2, John Zhang 3 risk factors related to DEACMP. It is scientific and 1. Department of Anesthesiology, Changhai Hospital, effective for ws to use mannitol reasonably and to give Secnod Military Medical University, Shanghai, China ACOP patients sufficient HBO treatment together with 2. Department of Diving Medicine, Faculty of Naval glucocorticoid, medicine of promoting blood circulation Medicine, Second Military Medical University, Shanghai, and removing bolld stasis, antiplatelet aggregation agent China and medicine of improving cerebral metabolism. 3. Department of Physiology and Pharmacology, Loma Linda University School of Medicine, Loma Linda, California, USA

P06 Objective: It is clear that hyperbaric oxygen Hyperbaric oxygen induces endogenous neural stem preconditioning (HBO-PC) provides spinal cord protection cells to proliferate and differentiate in against ischemia-reperfusion (IR) induced injury. This hypoxic-ischemic brain damage in neonatal rats study tested hypothesis that HBO-PC induces expression Yu-Jia YANG, Xiao-Li WANG, Chong-Feng CHEN, of endogenous antioxidant enzymes and anti-apoptotic Qing-Hong WANG, Yue YAO, Meng LI proteins Bcl-2 resulting in a motor neuronal mitochondrial Division of Neonatology, Department of Pediatrics, phenotype to prevent early apoptosis. Methods: Male XiangYa Hospital, Central South University, Hunan, China Sprague-Dawley rats were preconditioned with consecutive 4 cycles of 1-h HBO exposures (2.5 atmospheres absolute [ATA], 100%O 2) at a 12-h interval. enzyme activities by HBO preconditioning plays an At 24 h after the last HBO pretreatment, rats underwent 9 important role in the generation of tolerance against brain min of spinal cord ischemia induced by occlusion of the ischemia-reperfusion injury. descending thoracic aorta in combination with systemic hypotension (40 mmHg). Neurological function, TUNEL analysis and activities of caspase-3,9 were assessed during the first day after reperfusion. Mitochondria isolated P09 from spinal cord of non-preconditioned (control) and Hyperbaric oxygen prevents ischemia-reperfusion HBO-preconditioned rats were studied before or after injury of gut in rats after resuscitation from trauma spinal cord ischemia. Results: Spinal cord ischemia and hemorrhagic shock produced marked neuronal death and neurological Gang Wang 1, Lifang Jin 2, Weicheng Wang 2 dysfunction in animals. HBO-PC enhanced expression of Hyperbaric Emergency, Chinese Medical University, Mn-superoxide dismutase (Mn-SOD), catalase, and Bcl-2 Shenyang, China in the mitochondria in the normal spinal cord at 24 h after the last pretreatment (before spinal cord ischemia) and Background Hyperbaric oxygen (HBO) has been used retained higher levels throughout the early reperfusion in therapeutically in intestinal ischemia/reperfusion (I/R) the ischemic spinal cord. HBO-PC decreased superoxide injury models established by clamping superior and hydrogen peroxide levels in mitochondria and mesenteric artery, with ameliorating intestinal ischemic reduced cytochrome c release into the cytosol in the damage. This study was designed to investigate the lumbar spinal cord at 1 h after reperfusion. HBO-PC effects of hyperbaric oxygen (HBO) on the attenuated caspase-3 and -9, reduced motor neuronal ischemia-reperfusion (I/R) injury of gut in rats after apoptosis in the lumbar ventral horn, and improved resuscitation from trauma and hemorrhagic shock (T/HS) neurological function at 24 h after spinal cord ischemia. and elucidate the underlying mechanisms. Methods An HBO-PC increased nitric oxide (NO) production. iron mass from a retainable height smashed to the left L-nitroarginine-methy-ester (L-NAME, 10 mg/kg), a thigh of Wistar rat vertically, and at the same time, rats nonselective nitric oxide synthase (NOS) inhibitor, applied was bled to establish T/HS model and subsequently before each HBO-PC protocol, and abolished these resuscitated with shed blood and normal saline. Just prior beneficial effects of HBO-PC. Conclusion: We conclude to resuscitation, Wistar rats were randomly divided into 4 that HBO-PC reduced spinal cord ischemia-reperfusion groups: sham group, shock group, one HBO treatment injury by increasing Mn-SOD, catalase and Bcl-2, and by (HBOT) and three HBO treatment group. Results The suppressing mitochondrial apoptosis pathway. NO may be levels of lactic acid, induced nitric oxide synthase (iNOS), involved in this neuroprotection. nitric oxide (NO), and tumor necrosis factor-α (TNF-α) in intestinal tissue in one HBOT and three HBOT group after 24 hours from resuscitation were significantly lower than those in the shock group ( P<0.05); The histological injury P08 grading score of intestinal tissue in one or three HBOT Hyperbaric oxygen preconditioning induces tolerance group was lower than that in shock group and the against brain ischemia difference between them was significant ( P<0.05). Jiasi Li 1, Wenwu Liu 2, Suju Ding 1, Weigang Xu 2, Transmission electron microscopic photographs of Yangtai Guan 1, John H Zhang 3, Xuejun Sun 2 intestinal epithelium displayed that microvilli were Department of Diving Medicine, Faculty of Naval Medicine, hampered and lost, tight junction opened, mitochondrial Second Military Medical University, Shanghai, China crista disappearing, mitochondrium lysis, rough endoplasmic reticulum expansion, desmosomes Objective: The present study examined the hypothesis disappearing etc in shock group; whereas, the tight that cerebral ischemic tolerance induced by hyperbaric junction, desmosomes and mitochondrium appeared oxygen preconditioning (HBO-PC) is associated with an preserved in HBOT groups. All the above-mentioned increase of antioxidant enzyme activities. Methods: Male indicators of three HBOT group were better than those of Sprague-Dawley rats (250-280g, n=74) were divided into one HBOT group, but the difference was not significant sham, middle cerebral artery occlusion (MCAO) for 90 min, (P>0.05). Conclusion HBO therapy can improve oxygen and MCAO plus HBO-PC groups. HBO-PC was supply to intestine after resuscitation from T/HS, reduce conducted four times by given 100% oxygen at 2.5 the anaerobic metabolism, preserved tight junction and atmosphere absolute(ATA), for 1 hour at every 12 hours mucosal barrier, and decreased the production of interval for 2 days. At 24 hours after the last HBO-PC, inflammatory factor after resuscitation from T/HS, inhibit MCAO was performed and at 24 hours after MCAO, the excessive inflammatory reaction. Thus HBO prevented neurological function and Nissl Staining were performed to intestine and mucosal barrier from I/R injury after evaluate the effect of HBO-PC. Malondialdehyde (MDA) resuscitation from T/HS. content, activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) sampled from hippocampus, ischemic penumbra or core of cortex were measured. Results: HBO-PC improved P10 neurological recovery, lessened neuronal injury, reduced Kidney dysfunction caused by rat hind limb the level of MDA and increased the antioxidant activity of ischemia/reperfusion and effect of hyperbaric oxygen CAT and SOD. Conclusion: These observations on it demonstrated that an upregulation of the antioxidant Chun-jin GAO, Biao YUAN, Guo-zhong WANG, The laparotomy was performed. Bilateral hind limb Da-zhi CHEN ischemia was induced by applying an atraumatic clip at Beijing Chaoyang Hospital, Beijing, China the end of abdominal aorta adjacent to junction site of both femoral artery. At the end of the ischemic period, Objective: Acute limb ischemia is one of the most reperfusion of the limbs was achieved by releasing the challenging problems encountered by the contemporary clamp. The experimental animals was randomly divided vascular practitioner. Its incidence approximates 1.7 into three groups, and each group had 10 rats. (1) In cases per 10,000 population per year. Despite surgical sham group, the animals were subjected to the improvements in patient care and operative technique, the operation without clamping abdominal aorta. (2) In morbidity and mortality remain high, with mortality rates in ischemia/reperfusion group, after hind limb ischemia for excess of 25% and amputation in 20% of the survivors. 2h, reperfusion period was 1h. (3) In HBO group, after The high rate of morbidity and mortality of acute limb hind limb ischemia for 2h, this group animals were put into ischemia should be mainly attributable to the ischemia a model DWC150-300 hyperbaric oxygen chamber(HBO /reperfusion itself. Severe hypoperfusion of a limb, if left pressure in chamber is 0.2MPa)to be treated for 1h during untreated, will inevitably develop into tissue infarction and 1h reperfusion period. The other above groups were put irreversible cell death. Patients with acute occlusion of the into the normal pressure air. By using laser Doppler abdominal aorta are in an extremely compromised state. flowmetry (LDF), the microvascular blood flow was The patient's status will likely further deteriorate at the measured in kidney of the animals. The mean instant of reperfusion, and sudden cardiovascular collapse immunofluorescence intensity of CD31, CD61 or positive is commonly encountered with the release of accumulated percentage of CD62p were measured by the flow metabolic by-products into the circulation, which maybe cytometry(FCM). Ultrastructural histological pathological results in remote multiple organ injury, that is, multiple changes in kidney were studied. Results: Compared with organ dysfunction syndrome(MODS). The reason is that I/R group, the microvascular blood flow of the kidney in lower limb ischemia/reperfusion injury triggers a systemic HBO group was significantly increased, had significant inflammatory response (SIRS, systemic inflammatory statistical difference (P<0.05). Compared with surgical response syndrome). Morbidity and mortality following sham group, the expression of platelet membrane reperfusion of the acute ischemic limb may be a glycoprotein CD31,CD62p in I/R group was much manifestation of multiple organ dysfunction caused by a higher(P<0.05). The expression of platelet membrane systemic inflammatory response triggered by reperfusion glycoprotein CD31,CD62p in HBO group was much of the ischemic extremities. Hyperbaric oxygen(HBO) has decreased than that in I/R group(P<0.05). In I/R group, been used therapeutically in a variety of I/R injury models, extensive ultrastructural histological pathological injury of and the application of HBO to I/R disease appears kidney occurred. The ultrastructural histological effective. The basic mechanisms responsible for the pathological changes in HBO group was obviously beneficial effect of HBO in treating I/R injury are becoming alleviated. Conclusions: The expression of CD31 and better understood. HBO treatment may simultaneously CD62p on the platelet membrane significantly increased provide a beneficial effect directed at many components after rat hind limb ischemia/reperfusion model, indicating responsible for I/R injury, including neutrophil, the platelet -activating factor(PAF) was activated, which endothelium, inflammatory mediators, lipid peroxidation, partially result in inducing SIRS and remote multiple organ cellular energetics, and microvascular blood flow. But so injury. According to this study, early HBO therapy can far, there has been no literature on the application of HBO significantly decreased the expression of CD31 and to kidney dysfunction which results from acute limb I/R. CD62p on the platelet, indicating the PAF was inhibited, The purpose of this study is to determine whether there is maybe which stopped a series of PMNL activation ， potential protective effect of HBO on kidney dysfunction adhesion and systemic inflammation after reperfusion of which is associated with hind limb ischemia/reperfusion in ischemic hind limb. And the microvascular blood flow of rat model, and to discuss mechanism of HBO on it. the kidney in HBO group was significantly increased, the Methods: Adult male SD rats weighing 220 ～250g were ultrastructural histological pathological changes such as used in the study. Animals were kept under standard kidney in HBO group was obviously alleviated .As a kind condition. They were anesthetized with intraperitoneal of effective therapy , HBO can alleviate the pathological urethane(1.0g/kg) and secured in a supine position on a changes in kidney dysfunction caused by rat hind limb heating pad(which is controlled by a model CMA/150 ischemia/reperfusion model. Temperature Controller system); body temperature was kept at 37 oC after being shaved. Tracheostomy was performed with PE-240 tubing(1.67 mm internal diameter), which allowed spontaneous ventilation with room air .The P11 left carotid artery was cannulated with PE-50 tubing(0.58 Monitoring and disinfection of air culture in mm internal diameter), and arterial pressure and heart single-chamber with pure oxygen rate were monitored continuously on a model 7702B Xiufang MEI 1, Chaoqun TU 1, Xiaomei XIE 1, Hui LI 2, recorder with a 1280 series transducer. The right external Xiaodan CHEN 2 jugular vein was cannulated with PE-50 tubing(0.58 mm 1. The Shenzhen Peoples Hospital, Shenzhen, China internal diameter)for fluid administration. All animals were 2. The People’s Hospital of Longgang Region, Shenzhen, then allowed a 30-minute equilibration period. Intravenous China Ringer’s lactate was administered to maintain the systolic blood pressure > 90 mmHg throughout the experiment. Object: To evaluate the effect of chlorhexidine acetate Guiying He, Guanwen Zhang, Ping Yang, Wenping Han, solution on single-chamber with pure oxygen and to study Kefang Chen the convenient, effective, rapid and simple routine method Xinjiang Kelamayi Central Hospital, Kelamayi, China to prevent from cross infection in the single-chamber with pure oxygen. Methods: There were 5-7 sessions of HBO Objective: To observe curative effect of early hyperbaric in single-chamber with pure oxygen, in which it took oxygenation to pediatrics brain injury. Methods: the 60-80min and interval 10min between 2 sessions. It was clinical data of 51 cases of pediatrics brain injury in our disinfected by spaying with chlorhexidine acetate solution hospital from 1995,july to 2008.Feb were retro spectively after HBO. The air culture was done before, after every analyzed. Results: The cure rate of the curative group session of HBO, after disinfection for 5 consecutive days. and the control group were 78.27% and 52.17% Results: The average total bacterial count (TBC) before respectively, the results showed that the control group first HBO was 189±49cfu/m 3. The TBC shortly after HBO were better than those of the control group and the were more than1500cfu/m 3. Nevertheless, the TBC after difference was significant (p<0.05). Conclusion: Early 10min disinfected by chlorhexidine acetate solution were hyperbaric oxygenation to pediatrics brain injury is an less than 200 cfu/m 3. Conclusion : It is rapid, simple, effective method, which can raise cure rate reduce effective with chlorhexidine acetate solution on disabled rate and improve the prognosis . disinfection to single-chamber with pure oxygen.

P14 P12 Efficacy and nursing technique of hyperbaric oxygen Effects of preconditioning with hyperbaric oxygen on therapy on neonatal hypoxic-ischemic expression of GFAP and Nestin after spinal cord encephalopathy injury in rats Fei Lianfeng Peigang Lu, Hua Feng, Yongzhi Xia, Weihua Chu, Capital University of Medicine Affiliated Beijing Children’s Guoqi Gong Hospital, Beijing, China Department of Neurosurgery, Southwest Hospital, Third Military Medical University Objective: To explore the short-term effect of hyperbaric oxygen(HBO)based on conventional therapies in full-term Objective : To study the effects of preconditioning with neonates with hypoxic-ischemic encephalopathy hyperbaric oxygen on expression of GFAP and Nestin (HIE),and the effect of ameliorate sequelaes such as after spinal cord injury in rats at different time. Methods : deafness, cerebral palsy and mental retardation. Methods: fifty-five adult Sprague-Dawley adult rats, weighing from 100 full-term neonates with HIE were comprised, 52 were 250 to 300g, were randomly divided into 3 groups, administered with HBO and conventional therapies, and hyperbaric oxygen preconditional group(25,n=5) , normal the control group comprised 48 patients who were injury group(25,n=5)and control group(5,n=5). In the administered with conventional therapies only. Brainstem experimental groups, the rat spinal cord injury models auditory evoked potential and brain CT scan before, 3 were established by the way of Allen ,s method and the months after and 6months after management were expression and proliferation of nestin and GFAP at registered respectively. Results: The amelioration rate of different time(1 day, 5 day, 7 day, 10day, 14day) were Brainstem auditory evoked potential in treatment group observed with immunohistochemical staining and the and control group were 84%, 73% respectively. The analytical system of photographs. Results: In the normal amelioration rate of brain CT scan in treatment group and control group, the expression of nestin was hardly seen control group were 95%, 80% respectively. There is except ependymal cells of central canal, and the low distinct difference between the two groups (P<0.01). The expression of GFAP was seen. In the experimental groups, therapeutic effect was significant in HBO group than in the the nestin and GFAP expressions increased obviously in control group. Conclusion: HBO therapy has a marked the injured sites and adjacent sites 1 day to 5 days after short term effect in HIE patients and need to be used as injury, reached the peak value after 7days and followed by early as possible. Because of the severity of illness, gradual decrease from 10 days to 14 days. There were rapidness of changes and lack of response, the patients statistically significant differences in the nestin and GFAP need to be observed and nursing carefully while taking expressions between the preconditional group and normal HBO. injury group especially the control group. Conclusion The above results suggest that spinal cord injury can induce the expression of nestin and GFAP.HBO preconditioning can induce the proliferation and P15 differentiation of the neural stem cells in rats after spine Recent advance in researches of laboratory and clinic cord injury. on hyperbaric oxygen therapy and stem cell Chen Yifei Guangzhou City First Peoples Hospital, Guangzhou Medical College, Guangzhou, China P13 Observation on curative effect of early hyperbaric Hyperbaric oxygen therapy (HBO) is a relatively new oxygenation to pediatrics brain injury medical treatment that was developed in 1960’s and has been applied extensively in medical system now. It has been successfully used in treating a lot of diseases, such inhalation, but he patient’s condition was worse, restless, as carbon monoxide poisoning, decompression sickness, loss of sight, and right extremity paralysis after she left the gas Infarction and gas gangrene. There have been chamber. In this report, we will discuss: (1) Occurring numerous basic and clinical researches on HBO in the condition of cerebral air embolism; (2) Differential decades. However, the treatment mechanism of HBO diagnosis between cerebral air embolism and other acute should be explored further and detailedly. Human stem cerebrovascular diseases; (3) Compression treatment of cell (HSC) is one kind of cell that plays a very important air embolism; (4) Contraindications for treatment of bullae role in the development and growth of human body and and high pressure oxygen. possesses very strong self-renewal and differentiation potential. HSC can differentiate into almost all of cell types of adult body. Compared with traditional therapy, Application of HSC to treatment of human disease has P17 many advantages: lower toxin or non-toxin, long effective, Research progress of hyperbaric oxygen and free no immune repulsion when using self-HSC radical transplantation. Therefore, HSC has huge medical value Jun Wang, Chao Sun and extensive application potentials and has been a hot Qingdao Municipal Hospital, Qingdao, China spot of scientific research in the world now. Reseaches on HSC are focused in Embryonic Stem Cells, neural stem With the method of documentary data and consulting the cell and hemopoietic stem cell.Recently, a lot of relative literature at home and abroad in recent years, this researches from laboratory and clinic suggested that HBO paper makes a review on the study progress of Hyperbaric could mobilize marrow stem cell into peripheral circulation; Oxygen and Free Radical. Respectively, effects of it also could induce proliferation and differentiation of hyperbaric oxygen on free radicals, hyperbaric oxygen endogenetic neural stem cell and reduce death of might reduce the free radical in separately certain damaged stem cell. Clinical investigation showed that pathology situation, hyperbaric oxygen to be possible to HBO could effectively mobilize the hemopoietic stem cell increase the free radicals in the tumor organization, the of peripheral blood. Thus, it is causing tremendous relation of different oxygen exposure doses with free interest and attention of medical researchers that HBO, a radicals and the mechanism of hyperbaric oxygen safety, effectiveness, economy and practicality of therapy, preconditioning-induced ischemic tolerance in spinal cord has action on human stem cell. This article will review the with free radical relational several aspects has been advance in recent researches on HBO and stem cell, expounded. including 1. biological functions of stem cell; 2. recent laboratory and clinic researches about HBO impact on HSC; 3. potential applications of HBO impact on HSC to clinical treatment of diseases. This article will further P18 highlight the mechanism of HBO therapy and provide the The fast infection and isolation measures in newest information of HBO and HSC for medical workers. Hyperbaric Oxygen Therapy rescure procedure for gas gangrene patients injured by earthquake Huang Ling-Ling, Chen Shu-Lan, Zhang An-Qin, Nie Cai-Xian, Tang Ming-Chan, Zeng Yu P16 The medical science college of Sichuna province, Sichuan Report of a case with serious cerebral air embolism Provincial Hospital, Chengdu, China due to routine hyperbaric oxygen therapy, and successful rescue Objective: Observe the effect of fast infection and Xiaowen pan 1, shuyi pan 1, xiangen meng 1, huijun hu 1, isolation measures in Hyperbaric Oxygen Therapy rescue yu zhang 1, chen yang 1, mingxin li 1, qi zhang 1, hongbo liu 2, procedure for gas gangrene patients injured by wen zhong 3 earthquake. Methods: individual Hyperbaric Oxygen 1. General Hospital of Navy, Beijing, China Chamber into clean area, half-contaminated area and 2. Zhuhai People’s Hospital, Zhuhai, China contaminated area, working staffs wear isolation gown. 3. PLA No.422 Hospital, China During Hyperbaric Oxygen Therapy procedure, disinfect contaminated objects and floor with 500mg/l chlorine We report of a Case with serious cerebral air embolism antiseptic, ultra-violet ray radiation and 3%peroxyacetic due to routine hyperbaric oxygen therapy, and successful acid fumigation. Disinfect Hyperbaric Oxygen Chamber rescue. The patient is female, 44 years. At 2pm, June 3, repeatedly and take samples after therapy. Results: 2007, the patient entered 12-person-seat air pressure patients recovered, no crossing infection occurred, and chamber without oxygen inhalation, she did not feel chamber samples culture showed no bacteria grow. abnormally uncomfortable during compression and Conclusions: crossing infection can be prevented stabilizing pressure, and at 3 minutes before leaving effectively with strict infection and isolation measures. chamber, she suddenly developed dizziness, weakness, subsequently was unconscious, however, other patients had no abnormal condition in the chamber. Out of the chamber, subsequently, her consciousness exacerbated P19 gradually, which was considered as decompression The clinical efficacy of hyperbaric oxygen methods in disease. She was placed into high oxygen pressure the treatment of non-insulin-dependent diabetes chamber, with pressure of 0.3MPa, and received oxygen mellitus Shou-Chao LI, Chun-He LIU, Yong-Xin MA, of the people first arrived at high altitude, which was Yin-Chun ZHANG particular worked by increasing the SaO2 and PO2. People’s Hospital, Weifang, China

Objective: To study the clinical efficacy of Hyperbaric oxygen (HBO)methods in the treatment of P21 non-insulin-dependent diabetes mellitus. Methods: 163 The observation on tinnitus treated with hyperbaric cases of Non-insulin-dependent Diabetes Mellitus patients oxygen and ginaton were randomly divided into HBO group (87 cases) and Xiang-Hui Li, Yan Li, Xiao-Ying Luo, Hui-Min Sun hypoglycemic treatment group (76 cases), we tested General Hospital of Chinese People’s Armed Police blood glucose, HbA1c, insulin concentration, Cholesterol, Forces, Beijing, China triglyceride, and other indicators respectively in the two groups after 10 days, 20 days. Results: Blood glucose Objective: To discuss the clinical value of hyperbaric and HbA1c levels were significantly decreased in HBO oxygen and Ginaton in the treatment of tinnitus. Method: treatment group after 20 days, there was no significant From January 2002 to April 2008, 106 patients suffering difference between HBO treatment group and tinnitus were treated with hyperbaric oxygen and Ginaton. hypoglycemic treatment group (p> 0.05). At the same time, Pressure of hyperbaric oxygen was 0.2MPa, Breathing insulin concentration, HDL-C and APO-A levels increased oxygen 60 minutes, One time everyday, One period of significantly, and cholesterol, triglyceride concentration treatment include ten times, The treatment of every patient decreased significantly, there were significant differences is always 10-20 times. At the same time, Ginaton 20ml compared with drug treatment group (p 1c could transfusion, two times everyday. One period of treatment maintained for 20 days with similar levels (p> 0.05). is seven days, The treatment of every patient is always Conclusion: HBO methods are stable and endurable in 7-14 days. Results : The cure rate, the clearly effective the treatment of diabetic hypoglycemic, it also can rate and the total effective rate were respectively 12.3%, improve the fat metabolism disorder. HBO methods have 33%, 82.1%(P<0.01). Conclusion: It is suggested that the exact effect in the treatment of non-insulin-dependent hyperbaric oxygen and Ginaton have a better effect on diabetes mellitus. tinnitus.

P22 P20 Therapeutic effects and probable mechanisms of The effect of air pressure chamber treatment on SaO2 hyperbaric oxygenation protocol of durative-9-hour and arterial PO2 of people first arrival at plateau Qiuhong Yu, Yaling Liu, Yali Wu, Lianbi Xu Chun-yang He, Kai Zhou, Jian-wen Gu, Xi Fu Department of Hyperbaric Oxygenation, Beijing Tiantan Chengdu Military General Hospital, Chengdu, China Hospital, Capital Medical University, Beijing, China

Objective: To study the effect of air pressure chamber Objective: The aim is to assess the effectiveness of treatment on blood oxygen saturation, and arterial biood adjunctive HBOT in the treatment of permanent MCAO gas analysas of people first arrival at plateau. Methods: rats for only once continue 9 hours in super-early stage, 130 new enlisted soldiers were recruited. 65 of them were and to find one of the probable mechanisms of HBOT. enrolled as therapeutic group and the others as control Methods: (1) models and groups: Using the intraluminal randomly. The therapeutic group subjects were suture method, we subjected male Sprague-Dawley rats trentmented with air pressure to permanent middle cerebral artery occlusion model , chamber(0.1-0.12Mpa,40-50min ,once a day) after their then the rats were randomly divided into MCAO control arrival at plateau., .The test index included blood oxygen group and HBOT group, a sham control group and a saturation, arterial, blood gas analysis, morphology of sham-HBOT group also be used. And the groups were erythrocyte under the light microscope and anoxia divided into 13h and 5d subgroups. (2) HBOT protocol: symptom. Results: After the arrival at Lhasa, some After being modeled for 3 hours, rats in the sham-HBOT subjects suffered from anoxia symptom, were faded away and HBOT groups stayed in the hyperbaric cabin for 9 after laser treatment of air pressure chamber. The oxygen hours. They inhaled pure oxygen at the 1 st , 3 rd , 5 th , 7 th and saturation (SaO2)decreased in all subjects, from 98% at 9th hours, and hyperbaric air at the 2 nd , 4 th , 6 th and 8 th Chengdu airport to 80%(SaO2),PO2 decreased to hours. After being created into models, rats in the sham 50.3mmHg around at the evening of the day after arrival. control group and MCAO control groups breathed room air. The saturation automatically increased from the second (3) Main Outcome Measures: ① The Garcia neurological day gradually. But the group with air pressure chamber, grading systems were used on 3h, 13h, 24h, 3d and 5d to were significantly more than the control (p ＜0.01) . At the assess the therapeutic effect of HBOT. ② The infarction end of observation, the SaO2 restored to 97% around in volume was calculated with the TTC pathological staining treated group, and to 90% around in the control group (p<0.01).The PO2 restored to 83.6mmHg around in the on 13h and 5d points. ③ The expression of VEGF was control group,and to 50.3mmHg around in the control measured using immunohistochemical stainingon 13h and group(p<0.01). Conclusions: The treatment of air 5d points. Results: (1)The neurobehavioral outcomes pressure chamber can increase anoxia-resisting capability were improved with time going in all subgroups, there is significant different between 13h subgroups using Garcia Acknowledgments Projects supported by National scale(P<0.01).Also, neurobehavioral outcomes improved Natural Science Foundation of China (the Science Fund of after 13h in 5d subgrops, those of the HBOT group were the Chinese Academy of Sciences), Grant 30672240. more improved comparing with MCAO group, but there is no different (P>0.05). (2) Cerebral infarction volume of rats in MCAO group was larger on 5d than that on 13h(P<0.05), and the infarction volume was smaller in P24 HBOT group comparing with MCAO group on 5d, there is Three phenomenon and the theoretical explanation of significant different(P<0.05). (3) The expressions of VEGF hyperbaric oxygen medicine were increased in MCAO groups and HBOT groups. Xiao Pingtian HBOT groups showed increased VEGF expression Xiangya Hospital, Central South University, Changsha, significantly compared with MCAO group on 5d point China (P<0.01). Conclusions: (1) Durative-9h HBOT protecol was effect, HBOT inproved neurobehavioral outcomes In the natural world, it is common that many things can be and cerebral infarction volume, but the effectiveness was divided into three different parts, which are so called to be further defined. (2) Stimulate the more production of “three-morphous phenomenon”. Moreover, there are endogenous VEGF which related with capillary formation, always some inherent correlations and regularities with reduced infarction volume and neural protection is one of these three different forms of the same things. For the probable mechanisms of HBOT. example, the quantitative traits have three typic normal distributions and the objects have three typical movement modes. With regard to human body, there are three typical modalities of organs’ connection. “three-morphous phenomenon” does exist commonly in the field of hyperbaric oxygen (HBO) medicine, such as tristate P23 therapy, three states of substances, three stages and Therapeutic window of hyperbaric oxygen therapy three functions of HBO therapy, three methods of Xiao-Li WANG 1, Yan-Song ZHAO 2, Yu-Jia YANG 1, compression and de-compression, three laws of gas Qing-Hong WANG 1, Chong-Feng CHEN 1 getting into or out of the body due to the changes of 1. Division of Neonatology, Department of Pediatrics, environmental gas pressure. The study of XiangYa Hospital, Central South University, Changsha, “three-morphous phenomenon” in HBO medicine will Hunan, China further enhance our understanding the intrinsic laws of 2. Department of Ophthalmology, Weifang Medical HBO and benefit the patients for a better therapeutic University, Weifang, China effect.

Objective: Previous studies showed that hyperbaric oxygen (HBO) promoted cell proliferation in hypoxic-ischemic brain damage (HIBD) neonate rats. P25 Neural stem cells (NSC) existed in the brain lifelong and Hyperbaric oxygen therapy in professional athletes can be activated. This study was undertaken to assess muscle injuries. a series of 51 cases whether HBO treatment promoted the proliferation of NSC Philippe Colombani, Olivier Simon, Andréas Kauert, Bern and repaired the brain damage regardless of when it is ard Gamain, Jean-Marc Lapoussière, Marcel Chatel started, thus to explore the therapeutic window of HBO Nice Hyperbaric Oxygen Center, Pasteur University treatment. Methods: Seven-day-old Sprague-Dawley Hospital, Nice, France rats underwent left carotid ligation followed by 2 hours of hypoxic stress (8% O 2 at 37°C). Hyperbaric oxygen Background: Traumatic muscle injury affects muscular therapy was administered 3, 6, 12, 24, and 72 hours after fibers, but also conjunctive and vascular tissues. It HIBD. 5-bromo-2’-deoxyurindine and provokes lesions similar to an ischemia-perfusion 5-bromo-2’-deoxyuridine/nestin were detected by syndrome, reason why hyperbaric oxygen therapy (HBOT) immunofluorescence and nestin was examined by has been suggested to contribute efficiently to structural western blot analysis 10 days after HIBD. T-maze forced and functional recovery. However, until now, clinical alternation, the foot-fault test, and the radial arm maze studies were conducted only on delayed-onset muscle were conducted at P 22 days (14 d after HIBD), P 30 days, soreness (DOMS) and there is no study with statistical and P 34 days. Thereafter, cerebral morphology was evidence of improvement due to HBOT. Patients and examined by Nissl-staining 28 days after HIBD. Results: methods: Fifty-one professional athletes were treated in There were remarkable increases in the proliferation of Nice Hyperbaric Oxygen Center, among them, 67% were neural stem cells in the HBO-treated group, 3, 6, 12, and professional soccer players (mean age 29 +/-10 y). 24 hours after HIBD, as compared with the HIBD group. Wounds occurred during training or competition. They The HBO-treated group, 3, 6, and 12 hours after HIBD, concerned mostly lower extremities. Muscles injuries were performed better in the behavioral test and had less neural of various grade: pulled, strained or torned. Beside of loss in the hippocampal CA1 region as compared with the conventional local treatments, HBOT was prescribed HIBD group. Conclusion: The therapeutic window for (100% O2 at 2,7 ATA during 45 min; 2 sessions per day). effective HBO treatment could be delayed up to 12 hours Mean number of sessions was 12 (range from 1 to 31). after HIBD, while the effect decreased 24 hours after Results: Assessment criteria were clinical, radiological HIBD. and functional: 1. Clear reduction of the volume of the limb and rapid decreasing spontaneous or provoked pain level. Acknowledgments Projects supported by National 2. Echography or MNR imaging: reduction of size of Natural Science Foundation of China (the Science Fund of hematoma and inflammatory oedema. 3. Shortening of the the Chinese Academy of Sciences), Grant 30672240. resumption of training and quality of muscle strength. Conclusions: Although this was not a “control cohort” study, this series suggests that HBOT is apt to bring a clear-cut and fast improvement in clinical symptoms and P27 imaging findings after traumatic muscle injury. HBOT Expression of MCP-1 in brain of acute CO-poisoning performed in the early stages of intensive sport practice rats and development after HBO muscle injury provides a quicker and better quality of Xiaoping Xie, Jinsheng Li, Wenlan Wang recovery, which is highly important for professional Department of Hyperbaric Oxygen Treatment Center, athletes (and their coach!). Controlled randomized studies School of Aerospace Medicine, Fourth Military Medical should be done imperatively for confirming these University, Xi’ an, China preliminary results. Objective: To investigate the expression of monocyte chemoattractant protein-1 (MCP-1) and aggregation of monocytes/macrophages in brain of acute carbon monoxide (CO) poisoned rats and to provide new evidence for immunological mechanism of delayed neuropathological sequelae (DNS) after CO exposure. P26 Methods: 50 SD male rats were randomly assigned to Neural protection and toxicity of hyperbaric oxygen control, CO exposure for 1d, 3d, 7d and HBO treatment for on neonatal rats with hypoxic-ischemic brain damage 7d groups, and ten rats for each group. HE, Yu-Jia YANG, Bo-Xiang QI, Qing-Hong WANG, Miao JIN, immunohistochemical staining and reverse transcription - Meng LI, Yue YAO, Chong-Feng CHEN polymerase chain reaction (RT-PCR) were performed to Division of Neonatology, Xiangya Hospital, Central South study the expression of MCP-1 and aggregation of University, Changsha, China monocytes/macrophages in brain. Results: Expression of MCP-1 was very low in control group, which increased Objective: It is well known that high-concentration oxygen quickly after 1d of CO exposure. It reached peak value in (HO) can cause retinopathy of prematurity (ROP) and 3d exposure group, but decreased after 7d of exposure. In bronchopulmonary dysplasia (BPD). However, HBO treatment group, expression of MCP-1 decreased high-pressure oxygen (HBO) in the treatment of hypoxic significantly (P<0.01) as compared with that in 7d ischemic brain damage (HIBD) process is also toxic effect exposure group, but still higher than that in control group is not clear. The purpose of this study was to investigate (P<0.05). There was scarcely aggregation of the neuroprotection of HBO and its toxicity on HIBD monocytes/macrophages in brain of control rats. After 1d neonatal rats. Methods: Seven-day-old to 7d exposure of CO, the number of Sprague-Dauleys(SD)rats were randomly divided into six monocytes/macrophages-positive cell increased groups: (1) Control group; (2) HIBD group; (3) HO group; progressively and reached its peak value at 7d exposure (4) hyperbaric air group (HA); (5) HBO group; (6) group. Just as the expression of MCP-1, the number of HO+HBO group. The HA and HBO groups were monocytes/macrophages-positive cell decreased administered respectively with 2.0 ATA for 60 minutes of significantly (P<0.01) in HBO treatment group as air or oxygen in HIBD after 2 hrs, once daily for 7 days. compared with that in 7d exposure group, but still more The HO group was given 90% oxygen inhalation after than that in control group (P<0.05). Conclusions: the HIBD for 7 days. The HO +HBO groups were given 90% expression of MCP-1 and aggregation of oxygen inhalation and HBO daily for 7 days. The animals monocytes/macrophages may play a role in the were killed on the 21st., Results: Comparison with the happening of DNS after CO poisoning. HBO may alleviate CON group, the number of TUNEL positive cells in the damage of brain through decreasing the expression of hippocampal CA1 were increased significantly in the HIBD, MCP-1 and preventing the aggregation of HA and HO groups, but not obvious increase in HBO and monocytes/macrophages. HO+HBO. The number of nuclei of the new vessels was higher in HO and HO+HBO groups. Retinal stretched preparation vessels in HO and HO+HBO groups increased significantly, while there were no changes in P28 evidence in the other groups. Retina VEGF expression Effect of hyperbaric oxygen on acute focal cerebral was stronger in HO and HO+HBO group than other ischemia-reperfusion injury in rats groups. Lung tissue stained with HE showed that the Jinsheng Li, Yaoming Chang, Li Liu, Xiaoping Xie alveoli pulmonis were damaged obviously in HO and Department of hyperbaric oxygen treatment center, HO+HBO groups. Conclusions: HBO treatment has School of Aerospace Medicine, Fourth Military Medical neuroprotective effects on HIBD neonatal rats, but no University, Xi’ an, China toxicity on ocular and lung. Retinal vessel proliferation induced by high-concentration oxygen was related to the Objective: To investigate the effect of hyperbaric increasing of the VEGF expression., oxygen(HBO) on focal cerebral ischemia-reperfusion (IR) injury in rats. Methods: Rat model of focal cerebral ischemia induced by intraluminal filament occlusion of middle cerebral artery (MCA) was used. Histopathological groups in which the patients receive HBO oxygenation changes of infarct size, neuronal damage ，neutrophil after cerebral trauma for less 31 days recovered better the other one in which the patients receive HBO oxygenation infiltration and NOS positive neurons in the ischemic brain after cerebral trauma for less 30 days. Conclusion: The area after MCA occlusion for 1 hour and reperfusion for HBO combining therapy is an effective therapy for patients 4,11,23 and 71 hours were examined. During the with persistent vegetative state following cerebral trauma. reperfusion period, normal pressure pure oxygen The efficacy and regained consciousness was related with (99.5%O ) or 0.25 MPa (ATA) HBO treatment for 1, 2, 3 2 the beginning time of hyperbaric oxygenation. and 5 times were applied to respective groups and their results compared. Results: The infarct size, neuronal damage and neutrophil infiltration in normal pressure pure

O group were similar to those of ischemia-reperfusion (IR) 2 P30 group at 71 hours. Whereas the infarct size and neuronal Effect of early hyperbaric oxygenation on bone damage in HBO group after 5 times of HBO treatment at mineral density in spinal cord injured rats 71 h were reduced for 8.43% (level before optic chiasma), Min Liu, Min Tong, Xiangping Wu 11.20%(level of optic chiasma) and 8.66%(level behind The Second Xiang Ya Hospital of Central-South University, optic chiasma), and the numbers of neuronal damage in 5 Changsha, China high power (×400) fields (Nr/5F) were 11.36 (preoptic area), 8.94 (medial striatum), and 14.25(cortex) less as Objectives: To establish an animal model of spinal cord compared with IR group respectively. Besides, the injury (SCI),and to explore the effect of early hyperbaric neutrophil numbers (number/5 fields, 100×) in preoptic, oxygenation on bone mineral density (BMD). Methods: 36 striatum and cortex at the level of optic chiasma were 3.84, healthy 3-month-old female SD rats were 4.61, 2.56 at 5 h, 6.64, 7.34, 5.72 at 12 h and 9.78, 10.48, randomly divided into three groups: Sham group (10 7.84 at 24 h after the IR respectively; and they were much rats), SCI group (13 rats) and SCI+HBO group (13 rats). lower in HBO group at 12 h (5.01, 5.22, 4.03) and 24h Rats in sham group underwent laminectomy, while rats in (7.13, 7.21, 6.14) as compared with IR group (P ＜0.05, the other 2 groups underwent complete spinal cord respectively. The numbers of degeneration NOS positive transection at the level of the tenth thoracic vertebra. Rats neurons were increased gradually after MCA occlusion in in the SCI+HBO group were treated with hyperbaric brain area. After HBO therapy, the numbers of oxygenation 2th day after SCI ,1/d, 10 times as 1 course degeneration NOS positive neurons were obviously of treatment, for 3 courses, with a 6 days ，interval between decreased in cortex, preoptic area and medial striatum courses .All rats were sacrificed 7 weeks after operation, (P0.05). Conclusions: HBO (0.25MPa, 5 times) have BMD of lumbar spine (L ), every region of femur and significant effects of reducing infarct size, lessening 4-6 tible in vitro were all measured by dual-energy X-ray neurological symptom evaluation score, inhibiting absorptiometry (DEXA) using QDR-4500A equipments. neutrophil infiltration, inhibiting the degeneration of NOS Results: BMD in region 2 at the distal femur ,BMD in positive neurons and decreasing the neuronal damage in region 6 at the proximal femur and BMD in region 1 at the “penumbra” area on acute focal IR injury in rats. proximal tible in vitro were markedly decreased in SCI

group at 7 weeks post-operation compared with that in

sham-operated group (P ＜ 0.05~0.01) ,BMD of every P29 region at 7 weeks post-surgery in SCI+HBO group Effect of hyperbaric oxygen combined therapy on was slightly higer than that in SCI group , but no significant vegetative state following cerebral trauma in 124 difference between SCI+HBO group and SCI group was cases found . Conclusion: (1) The pattern of osteoporosis in Ronghao Yu, Qiuyou Xie, huai Huang, Yan Shen, complete spinal cord transection rats was succeed in Yanbin He, Lingling Sun experiment. (2) HBO could not obviously improve bone Guangzhou general hospital,Guangzhou Military loss in complete spinal cord transaction rats. Command, Guangzhou, China

Objective: To study the efficacy and influence factors of hyperbaric oxygenation (HBO) combining therapy on P31 patients with vegetative state (VS) following cerebral Comprehensive treatment of neonatal hypoxic trauma. Methods: One hundred and twenty-four cases of ischemic encephalopathy by sequential hyperbaric VS treated with HBO combined therapy from 2003 to oxygen therapy in the air-pressurized oxygen 2007 were analyzed retrospectively ，including the effects chamber: analysis of 257 cases Xiaoxin Lu, Huiping Peng, Yongjian Tiang, Weihong Fang, of treatment juncture on therapy and treatment times. The Xinru Hong, Maoying Lin, Chengfeng Wang pressure used for HBO was 0.2 MPa. The patients Department of Hyperbaric Oxygen Therapy, Fuzhou breathed pure oxygen for 80 min with an interval of 10 min General Hospital, Fuzhou, China in the middle. Once a day, twenty times was a course of treatment. Results: twenty-two(17.7 ％)patients were free Objective: To evaluate the effect of hyperbaric oxygen from the VS within 1 month. In 102 cases of persistent therapy (HBO) on the early phase of mandible lengthening vegetative state (PVS), the total effective rate was 90.20%. in the goat model. Methods: Six healthy goats were Fifty-seven patient regained consciousness (55.9%). Two randomly divided into two groups (n = 3 each). Animals underwent corticotomy of the hibateral body of the decreased (p ＜ 0.05-0.01); erythrocyte deformity index mandible after placement of distraction devices. Distraction, at a rate of 1 mm/day and a rhythm of 2 was significantly increased (p ＜0.05); both the scores of turns/day, began after a 5-day latency period for ten days. erythrocyte sedimentation rate(ESR) and erythrocyte Only the second group underwent HBO for 1.5 hours daily rigidity index were not statistically significant (p ＞0.05). for fourteen days from the second day of the latency More over, the average of neurological functional deficit phase. Twenty-eight days after completion of the distraction protocol, the animals were killed and the scales was more obviously decreased (p ＜0.01). The total distracted mandibles were harvested. The appearance of effective rate was 87.5 ﹪. Conclusion: Hyperbaric oxygen the samples, radiological changes, bone mineral density, and mechanical strengths and histology were examined. therapy is recommendable because of its operational Moreover, Serum alkaline phosphatase was measured safety as well as the therapeutic effect in treatment. before HBO and every four days thereafter from 3 days Hyperbaric oxygen therapy can not only obviously reduce pre-treatment till first day post-treatment. Results: 1. In the neurological deficit, but clearly improve the the experimental group, the surgery region swelling hemorheological properties. abated quickly; in the control group, surgery region in one animal had an infection. Every goat had bony callus, and the experimental group resulted in harder and wider P33 healing bone. 2. X-ray: All specimens showed a Change of cerebral metabolism rate of glucose in progressive calcification of the distracted area between patient of delayed neuropsychological sequelae after the mandibular segments, and the bone cerebral cortex CO poisoning following combining hyperbaric oxygen connected completely except the infected animal. The treatment distracted zone in the experimental group exhibited higher radiodense opacity. 3. Bone density showed that the Yu Gao, Chunjin Gao, Tie Wang, Huan Ge, Lianhua Wu, experimental group resulted in a denser healing bone Liming Zhao (P<0.05). 4. Three-point bending test showed that the Beijing Chaoyang Hospital, Beijing, China maximal loading in the experimental group was higher Objective: To estimate the effect of hyperbaric oxygen than in the control group (P<0.05) .5. In the control group, three zones exhibited progress of intramembranous treatment on cerebral metabolism rate of glucose(CMRglc) ossification. 6. Statistical analysis of the percentage of in patients with delayed neuropsychological sequelae after CO poisoning (DNS). Method: Thirty cases of DNS were bone trabeculae area in the distraction gap revealed that 18 experimental group showed a significant increase received F-FDGPET examination before hyperbaric oxygen treatment (HBOT) and after 40th HBOT compared with the control group (P<0.05). 7. Serum respectively. At the same time 17 healthy examiners were alkaline phosphatase: early after surgery, every animals 18 serumal alkaline phosphatase level increased, and in the also received F-FDGPET examination. The ratio of experimental group it increased more significantly than in mean radioactivity of cerebral lobe to that of cerebellum as the control group (P <0.05). Conclusion: HBO can semi-quantitative parameters was used to evaluate brain glucose metabolism. Result: The CMRglc of DNS patient enhance the anti-infection capacity of the surgery district and accelerate the rate of bone regeneration and decreased in bilateral frontal lobe 、parietal lobe 、temporal ossification, and increase the activity of serum alkaline lobe and occipital lobe before HBOT. After HBOT , there phosphatase. The domestic internal distractor has a were obvious improvement of CMRglc in bilateral parietal stable biomechanical property and good biocompatibility. lobe 、occipital lobe and left side temporal lobe and no

difference in bilateral frontal lobe 、right side temporal lobe. Conclusion: The CMRglc, decreased, on the early phase P32 of DNS. Combining hyperbaric oxygen treatment can Clinical study of the impact of hyperbaric oxygen improve CMRglc and cerebral function. therapy on hemorheology and neural function rehabilitation in patients with cerebral infarction Zhong Qiaofen Guizhou Provincial Peoples Hospital, Guizhou, China P34 Analysis of therapeutic effect of hyperbaric oxygen on Objective: To investigate the impact of hyperbaric oxygen idiopathic facial palsy on hemorheology and neural function rehabilitation in Fang Liu, Hua Su, Jia Chen treating cerebral infarction. Methods: Clinical observation First Affiliated Hospital, Fujian Medical University, China with 48 cases of cerebral infarction treated by HBO therapy. Compare the preselected indexes and contrast Objective: To analysis different degree and different the condition with those before therapy, then make a factors influencing on effectiveness of hyperbaric oxygen statistical analysis. Results: Compared with the (HBO) on patients with idiopathic facial palsy and to hemorheological indices before HBO therapy, whole blood evaluate the clinical significance of hyperbaric oxygen on viscosity in high, middle and low shear rate, plasma them. Methods: First, the influencing factors were viscosity, hematocrit (Hct), erythrocyte aggregation investigated statistically through a retrospective analysis index(EAI), erythrocyte electrophoretic time(EPT) and of total effective on 196 patients with idiopathic facial palsy, platalet aggregation rate(1,3,M) were more obviously including sex, age, basic disease, HBO treatment, house-brackmann facial grading and speed of signal HBO may promote the proliferation and differentiation of conduction of facial nerve. Secondly, according to the endogenous NSC in HIBD neonatal rats. different methods of treatment the patients were divided Acknowledgments Projects supported by National into group A (routine treatment) and group B(HBO Natural Science Foundation of China (the Science Fund of treatment), compared effectiveness of HBO treatment in the Chinese Academy of Sciences), Grant 30672240. different serious degree. Results: HBO treatment, house-brackmann facial grading and speed of signal conduction of facial nerve were major influencing factors. The total effective rate of group B was significantly higher P36 than that of group A (x2=3.96, P<0.05).No significant Acute air embolism with bilateral pneumothorax after difference was found between A and B group when a 24meter dive house-brackmann facial grading was Ⅱgrade, the same Chen Yang, Xiaowen Pan, Xuechang Li Navy General Hospital, Beijing, China result when speed of signal conduction of facial nerve was slight slower. The effective ratio of group B was higher A diver had an emergency ascent from 24m depth in a than that of the group A when house-brackmann facial dive accident, and suffered barotraumas with bilateral grading was Ⅲ~Ⅳ and Ⅴ~Ⅵ grade (P<0.01, P<0.05), pneumothorax and air embolism in CNS. When he was the same result when speed of signal conduction of facial sent to our hospital 19h after the accident, he was almost nerve was middling and serious slower (P<0.01, P<0.05). unconsciousness. This is the first case of barotrauma with The effective ratio of group B was higher than that of the bilateral pneumothorax in dive accident in our hyperbaric group A when the delayed treatment time was less than 3 center. The patient was successfully treated by days and more than 31 days (P<0.01). Conclusions: The recompression with HBO. It was effective for the air curative effect of HBO on different degree of patients with embolism of the CNS to treat with HBO even after 20h. idiopathic facial palsy is significant particularly in middling and serious degree patients. Receive HBO treatment early will help to improve herapeutic effect. P37 Analysis of 156 childhood Carbon monoxide poisoning managed by hyperbaric oxygen P35 Zhang Jin Effect of multiple course of HBO therapy on the Capital University of Medicine Affiliated Beijing Children’s proliferation and differentiation of neural stem cells of Hospital, Beijing, China HIBD neonate rats Qinghon Wang, Chongfen Chen, Men Li, Yue Yao, Objective: To discuss the characteristics of children Miao Jin, Yujia Yang withCarbon monoxidepoisoning (COP) , andhyperbaric Division of Neonatology, Department of Pediatrics, Xiang oxygen(HBO)therapy , in children. Ya Hospital, Central South University, Changsha, Hunan, Methods: Carboxyhemoglobin, (COHb) levels were not China parallel with severity of illness .Therapeutic pressure was due to the age of patient. Mild COP patients need 1~10 Objective: To observe whether to increase the times HBO process , moderate COP patients need 10~20 therapeutic course of HBO can promote neural stem cells times, severe COP patients need 20~30 times , all patient (NSC) proliferation and differentiation into neurons and to got recovery and follow up 2~6 months , no improve the efficacy of HBO therapy in HIBD neonate rat. sequelaes have been found yet. Results: Children COP Methods: Seven-day-old Sprague-Dawley rats underwent manifestations vary a lot, COHb levels can only be a left carotid ligation followed by 2 hours of hypoxic stress reference when judging the degree of COP. The prognosis (8% O 2 at 37°C). One, two, or three therapeutic courses o f will be good if HBO can be used as soon as possible and HBO (Group HBO-1, Group HBO-2, and Group HBO-3) long enough. Retrospectively analyzed 156 children with were administered after 2 hours later (2 ATA, once daily COP treated in Beijing Children’s Hospital HBO for 7 days as a therapeutic course). At 35 days, T-maze department in recent 3 years. Conclusion: COP occurs in forced alternation, the radial arm maze and foot-fault test, all ages of children, and can cause variety manifestations . BrdU/nestin and BrdU/NSE immunofluorescence, nestin Infant usually have untypical manifestations. protein (western blot) were performed. Results: (1) The number of BrdU +/nestin + cells of SVZ was largest increases in group HBO-3 compared with that in HBO-1 and HBO-2; (2)There was no obvious increase in P38 BrdU +/nestin + cells of DG in all HBO treatment groups; (3) Experience with non-survivors of acute carbon The number of BrdU +/NSE + cells of brain was largest monoxide intoxication who received hyperbaric increases in group HBO-3 compared with that in HBO-1 oxygen therapy and HBO-2; (4) Westen blot showed the express of nestin Te-Chun Hsia 1, Wan-Yun Hsiao 2 protein was highest in group HBO-3 than other groups; (5) 1. HBO Department, China Medical University Hospital, The HBO treatment groups performed better in the Taiwan, China behavioral tests with the increase in therapeutic 2. Respiratory Therapy Department, China Medical courses. Conclusion: To increase therapeutic course of University Hospital, Taiwan, China

Purpose: The main purpose of this study was to maintained. The femoral arteries and veins of determine the reason for the carbon monoxide (CO) anesthetized rats were cannulated for blood pressure, poisoning-related deaths at out hospital and to describe MAP and HR monitoring, and the arterial partial pressure - the demographic data and epidemiology. Methods: We of CO 2 (Paco 2), O 2 (Pao 2), SatO 2, HCO3 and PH value retrospectively selected this group of acute CO were measured via a blood gas analyzer. The brain intoxication patients that received emergency hyperbaric neurotransmitters expression was analyzed via oxygen therapy (HBOT) from April 2000 to August 2005 at stereotaxically implanted microdialysis probe, and lactate, our hospital. Data regarding age, gender, duration of CO glycerol, pyruvate and glutamate levels were measured. poisoning exposure, cause of the episode, underlying The blood-brain barrier (BBB) expression and brain disease, number of HBOT courses, hospital course, edema were measured by Evans blue method and brain cormorbidity and cause of mortality were obtained from dry/wet ratios respectively. We compared the extreme the medical records. We also reviewed the admission data hypobaric hypoxia group and normbaric nomoxia group records, including vital signs, Glasgow Coma Scale, for understanding the difference in physiological arterial blood gas, carboxyhemoglobin level, and characteristics. Result: The average survival time was intubation or not. Results: One hundred thirty-seven 29±5.4 min (n=10) for un-anesthetized rats; and 26±6.5 patients and 5 fatalities related to CO poisoning were min (n=10) for anesthetized rats in our extreme hypobaric reviewed; the mortality rate was 3.65%. The 5 fatalities hypoxia setting. The arterial partial pressure of CO 2 - were all male, in the prime of life (27~37 years old), and (Paco 2), O 2 (Pao 2), SatO 2, HCO3 and PH value without major underlying disease. Four (4/5) patients significantly decreased, and the neurotransmitter committed suicide by inhaling CO from burning charcoal. expressions in brain such as glycerol, glutamate and They all received emergent HBOT. Prolonged pyruvate/lactate ratio were significantly increased in unconsciousness was noted after series HBOT in 4 (4/5) extreme hypobaric hypoxia. We found no significant patients. Four (4/5) patients developed rhabdomyolysis difference in the blood-brain barrier (BBB) expression and and acute renal failure. The causes of death were multiple brain dry/wet ratios in our study. Conclusion: We report organ failure (3/5, 60%) and septic shock (2/5, 40%). the survival time and provide the evidence of changes in Conclusions: The causes of acute CO poisoning among physiological characteristics and brain neurotransmitters the fatalities were suicide by inhaling CO from burning of rats in extreme hypobaric hypoxia environment. The charcoal (4/5, 80%) and a fire accident (1/5, 20%) at our development of a new animal model for rats under hospital. They were in the prime of life without major extreme hypobaric hypoxia is advantageous for academic underlying disease. The brain is an oxygen dependent researches for diseases related to hypobaric hypoxia, organ, and the damage may be severe and irreversible such as acute mountain sickness. after CO intoxication and hypoxia. Rhabdomyolysis and acute renal failure may also occur. Secondary infection and septic shock may worsen the already poor condition. The cause of death may be considered as multiple organ P40 failure, including the brain, lung, and kidney. A thermodynamic mechanism for explaining the concurrence of joint ache and neurological deficit in one limb Cristian Melián P39 Asociación Chilena de la seguridad, Puerto Montt, Chile Survival and physiological derangement of rats in the extreme hypobaric hypoxia environment: Toward a Objective: To suggest a physiopathological mechanism new animal model of acute mountain sickness that explains the clinical observation of spinal Wang Chia-Ti 1, Niu Ko-Chi 2, Lin Mao-Tsun 3, cord-articulation congruency. Materials and methods : a) Chang Ching-Ping 4, Syong Ci-Huei 1, Lin Hung-Jung 1 Revision of records b) Analysis of Data. Results: 1. Department of Emergency Medicine, Chi-Mei Medical Thermodynamic hypothesis of probability of bubbles Center Tainan 710, Taiwan, China appearance in a tissue subjected to mechanical energy: 2. Department of Hyperbaric Oxygen, Chi Mei Medical “For any heterogeneous open thermodynamic system, Center, Tainan 710, Taiwan, China with non-adiabatic walls constituted by several 3. Department of Medical Research, Chi-Mei Medical non-compressible phases, with inert gases dissolved in Center, Tainan 710, Taiwan, China them and subjected to pressure, the probability of 4. Department of Biotechnology, Southern Taiwan formation of gas bubbles in the system when the pressure University, Tainan 710, Taiwan, China diminishes is directly proportional to the total energy (determined by factors such as thermal energy and mass Objective: To understand the survival and physiological of gas) accumulated by the system during the period of derangement of rats in the extreme hypobaric hypoxia time in which it was subjected to pressure; and inversely environment. Methods: Both anesthetized and proportional to the speed of energy transfer from the un-anesthetized male Sprague–Dawley rats (350-400g) system into the environment in the form of heat or mass of were exposed to extreme hypobaric hypoxia and, gas”. The specific place of bubble formation will be the normobaric normoxia. Hypobaric hypoxia was achieved by phase presenting a greater E and its surroundings. The a decompression chamber. The pressure was decreased skeletal muscle system, in the four limbs, receives a to 210 mmHg, which is equivalent to the pressure of 0.27 similar mass of gas. The diver applies work to the skeletal atm or at 9000m height, in a controlled rate. Survival time muscle system; for example, to the right arm: kinetic was measured after target pressure achieved and energy is generated which turns into an increase of internal energy and heat in the limb. The longer the time Activation of FGF-2/MEK/ERK 1/2/NF-κB and PKC/JNK the action is performed the greater the energy load and Pathways by Hyperbaric Oxygen Stimulated mass of gas. When the diver ascends the pressure proliferation in Osteoblast diminishes, the speed of elimination of gas mass and heat CHEN-PU HSIEH 1, CHING-YUANG LIN 2 is surpassed in the right arm, secondary to the energy 1. Orthopaedic Surgery Department and Hyperbaric accumulation during the task, with bubbles appearing in Center ,Changhua Christian Hospital, Taiwan, China tissues with a greater E and in its surroundings; for 2. Institute of Medical Research, College of Health example, in the right shoulder. In the spinal cord, Sciences, Chang Jung Christian University, Tainan, specifically in the synapse of the 1st and 2nd Taiwan, China motor-neurons in the right arm, a greater amount of heat and gas mass accumulates, the elimination speed rate is Background: To investigate whether the hyperbaric surpassed and bubbles appear. The specific phase for the oxygen(HBO) can promote the growth-arrest osteoblast spinal cord will then be the inter-synapse space and its (OB) cells to proliferate and differentiate, and to determine surroundings. The bubbles would cause a mechanical the probable mechanism .Methods: OB cells were blockade of the nervous stimulus that would be exposed to O2 with different levels of saturation and transformed into a sensitive or motor deficiency. So, the pressure for 3 days and 7 days. The OB cells were divided degree of neurological deficiency would be given by the into four groups: 1. TheControl Group (Group C ):cells number of synapses involved. Neurological recovery were cultured under ambient oxygen (21% O2) and would be given by: number of synapses involved, quantity, normal pressure(1ata). 2. ThePressure Group(Group size and time the bubbles interact in the synapses, P) :cells were treated with high pressure(2.5ata) only twice passing from a reversible mechanical-physiological daily. 3. High Oxygen Group(Group O): Cells were treated dysfunction to an irreversible anatomical alteration. with high concentration oxygen(50%) only twice daily. 4. Pressure and High Oxygen group(Group P+O): Cells were treated with high pressure(2.5ata) and high concentration oxygen(50%) twice daily. XTT was used to detect the cells proliferation and cell cycle progression P41 was determined by Flow analysis. Expression of growth Decompression sickness (DCS)-Is there a relationship factors was assayed by RT-PCR. In addition, we between the anatomical location of joint pain and determined HBO activated signaling pathway in OB cells location of injuries in spinal cord? by Western Blot analysis. Results: HBO therapy did Cristian Melián significantly promote OB cell proliferation and stimulated Asociación Chilena de la seguridad, Puerto Montt, Chile cell cycle progression after the cells had been treated for three days.Afterward, the effect attenuated day by Summary: A retrospective, cross-sectional and day. HBO also stimulated the OB cells to produce the descriptive investigation was realized in 35 patients with FGF-2 growth factors. Multiple signaling pathways, DCS Type I (joint pain) and Type II (neurological deficit) in including FGF-2/MEK/ERK 1/2/Akt/p70 s6k /NF-κB and the same episode of DCS. The patients were treated in PKC/JNK, are involved in the proliferation of OB cells by “Unidad de Baromedicina ACHS Puerto Montt (Chile)”, in HBO stimulation . Conclusions: The pressure and high the period between March 2006 and March 2008. A saturation of pressure have positive effects on stimulating registry of the anatomical location of joint pain and grow-arrested OB cells to proliferate and differentiate neurological deficit was realized, dividing the body in 4 through activation of FGF-2/MEK/ERK 1/2/Akt/p70 s6k quadrants. There were defined four types of relation of /NF-κB and PKC/JNK signaling pathway.However,we congruence. 1) Congruence of quadrant 2) Congruence of were unable to determine between air pressure and side 3) not demonstrable Congruence 4) there is not oxygen concentration which is the more important factor.It congruence of quadrant. Objective: To describe the is believed that HBO can be useful for fracture healing in existence of a relation between the anatomical location of clinical application in an optimal situation. the joint pain and the location of the lesion in spinal cord (spinal cord - articulation congruency). Materials and Methods : It made a review of records and analysis of data obtained from each patient. Results: A congruence of P43 side (spinal cord - articulation congruency) was observed Hyperbaric oxygen therapy for chronic refractory in the 100% of the cases, in which it possible osteomyelitis of the sternum: a case report demonstrated positively or negatively congruence. In 80 Raymond C Shields, Francis C Nichols, William % of these cases the congruity Medular - articulate is in G Buchta, Paul L Claus the same quadrant. In 0% of the cases “there is not Mayo Clinic and Mayo Foundation, Rochester, MN, USA quadrant congruence”. Conclusions: A relation between the anatomical location of the joint pain and the location of Objective: To review a case of chronic refractory the lesion in spinal cord was observed. osteomyelitis of the sternum. Methods: A 32-year-old female with a history of lye ingestion at 14 months of age underwent multiple esophageal surgical procedures including subcutaneous colonic interposition with P42 conversion to substernal colonic interposition requiring median sternotomy, revision of pharyngocolostomy, and eventual revision of cologastric anastomosis for obstruction. The last surgical procedure was complicated suggests that prior concerns regarding traditional cytotoxic by a nonhealing draining sinus tract at the sternum. chemotherapy are not justified by the published Clinical and computed tomography findings were experience. Special considerations do relate to Bleomycin consistent with chronic sternal osteomyelitis without where reports document occasional but potential life anastomotic leakage. Narcotic analgesics were frequently threatening pulmonary fibrosis in patients exposed to required for pain management. Hyperbaric oxygen oxygen-enhanced breathing gasses even some time after therapy (HBOT) was initiated. Twenty four of 40 Bleomycin administration. Some drugs may be potentiated recommended sessions were completed which included a in their cytotoxic effects on cancer cell growth though this profile of 100% oxygen administered via an oxygen hood application has not been widely investigated or adapted to (Sea-Long, Series 500 Hood / Neckdam System, clinical advantage. For some of the newer drugs such as Louisville, KY, USA) at 105 kPa (2 atmospheres absolute, Bevacizumab and Cetuximab, there is not yet an 33 fsw) for 90 minutes with a 5 minute median air break. adequate published experience to make definite All sessions were conducted in a multiplace chamber recommendation; however, based on their mechanisms of (Fink Engineering TL24-165 AH11, Queensland, action, these and other drugs with similar mechanism are Australia). Ertapenem antibiotic therapy was continued likely to interfere with the hyperbaric oxygen delivered to throughout HBOT and discontinued at completion. No enhance wound healing or treat delayed radiation injuries. surgical debridement was required. Results: Dramatic Conclusions: The interaction between hyperbaric oxygen pain relief was seen early along with cessation of sternal and cancer chemotherapy is complex and not well-studied. drainage. Upon completion of 24 sessions, the sternal Older recommendations that absolute contraindications wound had healed completely. The C-reactive protein exist for most traditional drugs are not justified by improved from 16.1 mg/L before HBOT to less than 3 available published literature. For newer drugs targeted mg/L at completion. Conclusion: HBOT is beneficial for against growth factors, the mechanism of action suggests chronic osteomyelitis by reversing tissue hypoxia, that they will inhibit hyperbaric oxygen’s salutatory effect enhancement in leukocyte bacterial phagocytosis, and on wound healing. improving tissue perfusion. These mechanisms appear to have been important in the healing of chronic sternal osteomyelitis in this patient. Reports of HBOT for sternal osteomyelitis remain limited in the English literature. P45 Further investigation with well-designed clinical trials of Analysis of dynamic state of rheo-encephalogram HBOT for this indication is necessary to substantiate this during the procedure of hyperbaric oxygen therapy treatment Hongjun Wang 1, Qibiao Weng 1, Lingling Chen 1, Xuelin Chen 1, quyun Yang 2, Jin Liu 1, Guimei Zhou 1 1. Zhujiang Hospital attached to Southern Medical University, Department of Hyperbaric oxygen, Guangzhou, P44 China Hyperbaric oxygen and concurrent chemotherapy: 2. The First Hospital attached to Guangdong what are the concerns? Pharmaceutical College, Guangzhou, China John Feldmeier 3. Shilong People’s Hospital of Dongguan City, University of Toledo Medical Center, Radiation Oncology Guangdong Province, Guangzhou, China Department, Toledo Radiation Oncology, USA 4. The Second People’s Hospital of Nanhai District, Foshan City, Guangdong Province, Guangzhou, China Objective: To review the published literature and putative effects of cancer -chemotherapy on patients receiving Objective: Three dynamic states of rheo-encephalogram hyperbaric oxygen. Methods: Electronic computer data (before HBOT, during HBOT and after HBOT) of patients base literature searches as well as manual searches of who received the HBOT (hyperbaric oxygen therapy) were known references in hyperbaric medicine were employed analyzed, so as to observe and analyze the effect of the in order to define the potential interactions of hyperbaric whole procedure of HBOT on function of cerebral vessels, oxygen and chemotherapy in cancer patients undergoing to know about the diversity and rule of function of cerebral active treatment. In the past decade several new drugs vessels and change of cerebral blood flow, and to provide with new mechanisms of action have become available. theory of HBOT on cerebral diseases. Method: These drugs may target growth factors such as VEGF Rheo-encephalogram was done in the following states: in (vascular endothelial growth factor) or EGF (epithelial normal atmospheric pressure, in 0.2 MPa, after growth factor) while traditional cytotoxic chemotherapy is 40-minute’s HBOT, in normal atmospheric pressure after designed to directly disrupt cancer cell proliferation by the whole procedure of HBOT. All data is analyzed by interfering with mitosis. Results: The interaction between SPSS 11.5. Result: Differences are significant in the hyperbaric oxygen and cancer chemotherapy is complex following items: resistent index, rise time, whole recovery and not well-studied. Older recommendations that time, elasticity index, increased angle, top included absolute contraindications exist for most traditional drugs angle. Items of rheo-encephalogram of internal carotid are not justified by available published literature. For artery and vertebrobasilar artery are dissimilar between newer drugs targeted against growth factors, the “patients after inhale oxygen, patients go out the mechanism of action suggests that they will inhibit hyperbaric chamber” and “before pressure is elevated, hyperbaric oxygen’s salutatory effect on wound healing. after pressure is stable”, while differences are not More study is needed before definite recommendations for significant between “before pressure is elevated” and these new drugs can be made. The review of these issues “after pressure is stable”, so do “patients after inhale oxygen” and “patients go out the hyperbaric chamber”. history,, clinical manifestations, and adjunctive Conclusion: HBOT plays a positive role in vascular examinations such as, hemoglobin resistance and angiotasis when HBOT was received, level(Hgb), hematocri(Hct), computerized tomography, while HBOT exerts no influence on volume of blood flow of (CT), and magnetic resonance image (MRI). All cases cerebral vessels. It is indicated that change of vascular received the, combined treatment :the USN treatment resistance and angiotasis is mainly affected by stimulation table 6A and subsequent, HBO2 therapy (50 ft/ 120 min); of high pressure oxygen on vascular smooth muscle acupuncture of head, neck, hand, abdomen, back, buttock, rather than elevated oxygen pressure. Advanced research leg and blood letting of Wei-Chung, acupuncture point and is required on cause of cerebral vessels’ contraction physical therapy. Results: Four male fishermen had a without reduction of volume of blood flow and duration of repetitive dives to the depth, (25-52 m) for 40-100 min with effects of elevated oxygen pressure on function of SCUBA or surface supply compressed air., All dives cerebral vessels. exceeded no decompression limit without adequate, decompression stop . The clinical symptoms included changed, mentality, ptosis of eyelid, numbness and weakness of upper limbs or, lower limbs, difficulty in P46 urination and defecation. Two cases had increased Hgb 18F- FDG PET imaging before and after hyperbaric and Hct. One CT scan showed the brain edema and two oxygen combined therapy in persistent vegetative had, positive MRI findings of brain and cervical spine. state After the combined, treatment, three cases (75%) had Yu Ronghao, Xie Qiuyou, He Yanbin,et al. complete recovery of motor function, and walked smoothly Department of Hyperbaric Oxygen, Guangzhou general leaving the hospital. One serious case could walk, for a hospital, Guangzhou Military Command, Guangzhou, short distance with the walking stick. Hypoesthesia over China lower, trunk improved markedly. Two cases had urination by themselves and, smooth defecation. The other two had Objective: To evaluate the therapeutic effect of sensation of urination and, increased power of anal hyperbaric oxygen combined therapy in persistent sphincter but still needed urinary catheter, and laxative. vegetative state, (PVS) patients with 18 F- The hospital stay was all within 30 days for four cases. fluorodeoxyglucose (18 F- FDG) position emission Conclusion: From these clinical results, we suggest that tomography (PET) imaging. Methods: Ten patients with this, combined treatment of acupuncture, HBO2 and PVS received 18 F- FDG PET imaging examination before rehabilitation may be, another useful choice in the and after hyperbaric oxygen combined therapy. Results: management of neurological DCS. All of the 10 cases were with abnormal cerebral glucose metabolism. Some abnormal cerebral glucose metabolism areas were concordant with the changes on brain CT or MRI,, but some were not. As PVS recovered, P48 improvement of cerebral glucose metabolism or complete Mechanically ventilated patients poisoned with return to normal were also observed., Conclusions: 18 F- carbon monoxide: outcome after hyperbaric oxygen FDG PET imaging is a safe and reliable modality, which therapy not only can directly reflect the level of cerebral cerebral Wang Chia-Ti 1, Cheng Chin-Jen 1, Liu Te-Ming 1, glucose metabolism and serve as a supplementary work Niu Ko-chi 2 up to brain CT and MRI, but also can be used to evaluate 1. Department of Emergency Medicine, Chi Mei Medical the therapeutic effect of hyperbaric oxygen combined Center, Tainan, Taiwan, China therapy in PVS and to predict the prognosis. 2. Department of Hyperbaric Oxygen, Chi Mei Medical Center, Tainan, Taiwan, China

Objective: To review the outcome of severely poisoned P47 patients who receiving mechanical ventilation and HBO Combined treatment of hyperbaric oxygen therapy, therapy. Methods: We reviewed the case records of 16 acupuncture and rehabilitation in four cases of patients required mechanical ventilation for severe carbon neurological decompression sickness monoxide poisoning treated with HBO at Chi-Mei Medical Hui-Chieh Lee center between May 2001and December 2005. We Zuoying Armed Forces General Hospital, Taiwan, China obtained numbers of days admitted in ICU and wards, underlying diseases, laboratory data. We analyzed the Objective: The neurological decompression sickness mortality and the morbidity in these treated patients. Only (DCS) may cause severe symptoms and sequelae such patients without any other comorbidity, like trauma or burn as paraplegia and difficulty in, micturition and defecation. injury, can be included in our study. Results: Fourteen Traditional treatments includes United, States Navy (USN) (87.5%) of 16 patients committed suicide by burning treatment tables, hyperbaric oxygen (HBO2) therapy, & charcoal, 1(6.25%) was poisoned by car exhaust and rehabilitation. In order to improve the therapeutic effects, 1(6.25%) was poisoned by faulty water heating we, tried to use a combined treatment of HBO2 、 installations. All patients received endotracheal tube insertion before HBO therapy and HBO therapy acupuncture &, rehabilitation in the neurological DCS . subsequently. Although 15 patients received HBO therapy Methods: Four cases of neurological DCS were collected, within 2 days after admission, one was treated on the 16 th via our emergent room and diagnosed by means of diving day after admission due to his unstable condition. Mean ICU stay was 7 days and total hospital stay was 16.7 days. to a prospective clinical study to evaluate the therapeutic There was 1 mortality case, and the other three were also benefit of hyperbaric oxygen therapy (HBOT) in a large, defined as poor outcome due to their vegetative status. patient population. Moreover a, first five years follow-up Twelve (75%) patients who underwent HBO therapy were study, should give a prediction for the stability of the discharged without severe sequelae. Conclusion: results after therapy. Method: Within the past 8 years 266 According to our experience, mechanically ventilated patients with MRI confirmed diagnosis of BME or AVN CO-poisoned patients who receive hyperbaric oxygen had were evaluated. They were classified according to the a fair prognosis. Although the risks for the mechanically Association for Research of Circulation Osseous (ARCO), ventilated patients are relatively high, HBO therapy knee joint (n = 207) and ankle joint, (n = 59). Clinical seemed to reduce the risk of serious neurological deficit in examination, (range of movement , pain score) was done severe CO poisoned patients. before, during and after therapy and within the follow-up period. MRI control after, HBOT was classified for results. The number, of HBOT sessions (250/60) varied, according to, the clinical, success (minimum 15 or more). P49 Classification of Result: 3+ no complaints/ 2+ clear Experience in the treatment of decompression approvement 1+ less 0 no progress. In the follow-up study sickness from the mountain lake diving in Taiwan group (FSG) n = 104, patients with interaction of Shyanher WANG interfering factors (e.g. operation, arthrosis) were Department of Occupational and General Medicine, excluded to evaluate, the real effect of HBOT stability (n = Tungs’ Taichung MetroHabor Hospital, TaiZhong, Taiwan, 64). Results: Mean age knee group (KG) was 57 years in China comparison to the talus group (TG) 40 years. Number of sessions 27(KG) and 25 (TG) Clinical outcome ARCO Decompression sickness (DCS) occurred in the mountain stage 2, KG : 77,2% rated 3+, 19% rated 2+ in comparison lake is extremely rare in Taiwan., However, there were 3 with outcome stage 2 TG: 64,3%, 3+, 28,6 2+. Clinical victims during a 6-day rescue dive after the Mindulle outcome ARCO stage 3 knee group 60% 3+ versus 42,2% typhoon attacks., It damaged the water gate in the Carp TG. The MRI outcome varies significantly in this stage: 3+ Reservoir where located 300 meters above sea level. rated in 38,4% versus 9,1% TG. 94 % in the FSG Eleven male participated the dive., Their average age, answered our questionnaire. 81% remained in an body weight and diving experience were 25 year-old, 72 unchanged therapeutic outcome, improvement in 6% and kg and 3 years, respectively., The rescue started 24 hours symptomatic aggravation also in 6%. Most of the changes after ascending., The surface-supply dive was adopted were, seen within the first year. Conclusion: HBOT due to the limited time and space., The bottom time was administered as a conservative causal therapy leads to 30 minutes and the depth 60 meters under the mountain very good results in earlier ARCO stages particularly, in lake., Two dived each time. The patients were treated with the knee joint. There is a significant difference in MRI the modified and extended recompression tables rating in stage 3. The therapeutic follow-up outcome according to the individual condition. One victim with bend already shows very stable results within the first year. over multiple joints was treated with a modified table 5A., He got complete remission after the 5-hour treatment., The other two victims with bend and numbness over multiple limbs were treated with a modified table 6A for 8 P51 hours., Because both had some residual symptoms, a Effect of hyperbaric oxygen therapy on bone table 5 was added on the next day., Both got complete regenerating ossification during mandibular remission thereafter., All three victims received no other distraction osteogenesis in goats medicines except of isotonic saline., None of them has Mingxing Li 1, Xiaoxin Lu 1, Jie Zheng 2 late complication to date. The relative high incidence of 1. Department of Hyperbaric Oxygen, Fuzhou General DCS in this mountain lake dive might be from the hostile Hospital, Fuzhou, China. environment. The modified table 5A and table 6A, added 2. School of Stomatology ， Fujian Medical University, with a followed table as necessary, might be tailored to Fuzhou, China optimize the treatment of the DCS occurred in the mountain lake. Objective: To evaluate the effect of hyperbaric oxygen

therapy (HBO) on the early phase of mandible lengthening

in the goat model. Methods: Six healthy goats were

randomly divided into two groups (n = 3 each). Animals P50 underwent corticotomy of the hibateral body of the Hyperbaric oxygen treatment of bone marrow edema mandible after placement of distraction devices. (BME) and avascular osteonecrosis (AVN) of knee Distraction, at a rate of 1 mm/day and a rhythm of 2 joint in comparison to talus. first long term results (5 turns/day, began after a 5-day latency period for ten days. years) in therapeutic outcome Only the second group underwent HBO for 1.5 hours daily Johannes von Reumont, Anke Fabian for fourteen days from the second day of the latency Hyperbaric Centre, Heidelberg, Germany phase. Twenty-eight days after completion of the

distraction protocol, the animals were killed and the Objective: Positive effects in the BME and AVN treatment distracted mandibles were harvested. The appearance of of the knee joint were first-time observed during a pilot the samples, radiological changes, bone mineral density, study in our centre, in 1998 and 99. These results entailed, and mechanical strengths and histology were examined. Conclusion and Discussion: The extensión of the time Moreover, Serum alkaline phosphatase was measured the divers spend on the surface after each dive, thanks to before HBO and every four days thereafter from 3 days the conclusión of Breath Hold Apnea Table for Lower pre-treatment till first day post-treatment. Results: 1. In Embolism has made accidents due to the formation of the experimental group, the surgery region swelling bubbles caused by the excess of nitrogen accumulated in abated quickly; in the control group, surgery region in one the time of diving almost extinct. animal had an infection. Every goat had bony callus, and the experimental group resulted in harder and wider healing bone. 2. X-ray: All specimens showed a progressive calcification of the distracted area between P53 the mandibular segments, and the bone cerebral cortex Summarization of 3180 children with hyperbaric connected completely except the infected animal. The oxygen therapy distracted zone in the experimental group exhibited higher Chen Yanhua, Capital University of Medicine Affiliated radiodense opacity. 3. Bone density showed that the Beijing Children’s Hospital, Beijing, China experimental group resulted in a denser healing bone (P<0.05). 4. Three-point bending test showed that the Objective: Hyperbaric oxygen department of Beijing maximal loading in the experimental group was higher Children’s Hospital was founded in 2001-6-1. It has been than in the control group (P<0.05) .5. In the control group, 7 years since then. Our department has become the three zones exhibited progress of intramembranous biggest pediatric HBO unit in Beijing region. Now we have ossification. 6. Statistical analysis of the percentage of 2 adult pure oxygen chanbers, 5 infant chanbers. There bone trabeculae area in the distraction gap revealed that are 50 person-times per day on average and now we have experimental group showed a significant increase treated over 70000 person-times safely, including more compared with the control group (P<0.05). 7. Serum than 10 kinds of disease. Methods: Now we summarize alkaline phosphatase: early after surgery, every animal's our experiences of management of all kinds of disease in serumal alkaline phosphatase level increased, and in the children as follows: Neonatal hypoxic-ischemic experimental group it increased more significantly than in encephalopathy, Cerebral paralysis, Zentrale koordination the control group (P <0.05). Conclusion: HBO can storung, Virus encephalitis, Carbon monoxide poisoning, enhance the anti-infection capacity of the surgery district Virus encephalitis combined with cortex blindness, and accelerate the rate of bone regeneration and Congenital heart disease after operation, Exterior ossification, and increase the activity of serum alkaline waterhead, Sudden deafness, Brain trauma, Toxicosis phosphatase. The domestic internal distractor has a encephalopathy ,crushing syndrome etc. Age: the stable biomechanical property and good biocompatibility. youngest patient is 8 hours after birth, the oldest is 18 years. Therapeutic scheme: Therapeutic pressure range from 0.05MP to 0.1 MP. Result: According to our experiences, pediatric HBO is a valuable method for most P52 cases, especially for those Carbon monoxide poisoning, Breath hold apnea tables for lower embolism (BATLE) Virus encephalitis combined with cortex blindness, experience after more than six years 2000 – 2008 drug toxicosis, HIE, crush syndrome and virus John M Batle encephalitis. Conclusion: We consider that pediatric MEDISUB Hyperbaric & Underwater Research Institute HBO is a very effective and safety method as far as strictly Palma de Mallorca, SPAIN abide the rules and criterions. The sooner the management begin, the better the result will be. In the Balearic Islands, there is a great number of people interested in submarine fishing. Although fish are becoming scarcer to find, and the bigger ones are at a deeper depth which leads to the use of electric scooters ( submarine scooters) arriving to depths of up to 63 metres in apnea in the space of approximately two minutes to the said pressure with intervals on the surface of about two or three minutes.The number of dives per hour is about fifteen and each sesión is usually done over a period of between three to eight hours. Materials and Methods: Since l994 to present date, a study has been made of about forty cases of disbaric diving accidents in apneaatended by Medisub Hyperbaric Institute during the period l995 -2005. In the year 2000, the first tables of Breath Hold Apnea?Tables?for Lower Embolism ( BATLE ) were published at the EUBS Congress in Malta, and the second generation of tables were published in ICHM 2002. Now with more than six years of practise and experience of submarine fishing with the said tables. Results: The number of accidents diminished progressively and since then, few accidents have been presented in divers practising submarine fishing.

刊载交流 Publish Only K01 Objective: To study the change of apoptotic cells and Observation of hyperbaric oxygen therapy’s effects expression of bcl-2 protein, Bax protein and caspase3 on delayed encephalopathy after acute carbon protein in mice brain hippocampus after acute carbon monoxide poisoning monoxide poisoning (ACMP). Mathods: 56 Male Li Li Xu, Yi Liu, Hong Mei Chen KunMing mice were devided into seven groups (control Taihe Hospital Affiliated To Yunyang Medical College, group, CO-exposed 1h group, CO-exposed 6h group, Hubei, China CO-exposed 12h group, CO-exposed 24h group, CO-exposed 3d group and CO-exposed 7d group), eight Objective: To discuss the effects of hyperbaric mice each group. The control groups were exposed to air oxygenation in delayed encephalopathy after acute and the poisoning groups were exposed to CO. General carbon monoxide poisoning. Methods : 50 patients were TUNEL, immunohistochemistry stain and flow cytometry divided randomly into control group (19) with drage were used to observe the neurological damage and the therapy and experimental group(31) with hyperbaric change of caspase3, bcl-2, Bax protein expression. oxygen therapy based on drage treatment . And then to Results: TUNEL staining positive cells significantly evaluate the effects after 1courses, 2courses and increased on 3d in hippocampus after acute CO 4courses of theatment. Results : The efficacy of poisoning(P<0.05), and got much higher value on experiment group is obviously difefent (P <0. 05), the 7d(P<0.01); caspase3 protein expression increased at 1h curing time of experiment group was shorter than that of after poisoning, reached the highest at 24h and recovered the control group, the result is signicant different (P <0. on 7d; bcl-2 protein expression increased at 1h in 05). Conclutions : Full dosage HBO therapy has obvious hippocampus after acute CO poisoning, peaked at 24h curative effect on delayed encephalopathy after carbon and recovered on 7d; Bax protein expression increased at monoxide poisoning. 1h in hippocampus after poisoning, peaked at 24h and recovered on 7d. Conclusions: There existed delayed neurological apoptosis and apoptosis-associated factors expression in mice brain hippocampus after acute CO K02 poisoning. Celluar apoptosis might be one of the The observation of curative and nursing the children pathological mechanism of delayed encephalopathy after brain damage by Hyperbaric Oxygenation acute carbon monoxide poisoning. Dao-dong Liu, Dao-lin Liu 1. The people’s hospital of Lu’an city, Anhui province, Lu’an, China 2. Luan City Hospital in Anhui Province, Lu’an,China. K04 Hyperbaric oxygen therapy to the resumption of Purpose: To observe the curative effect of the hyperbaric mycoplasma encephalitis patients 1 case oxygen action used for treating craniocerebral injury of Wencheng Liu, Haiying Jia, Xin Yang, Juan Song children and sum up the experience of nursing care. Beijing 306 Hospital of PLA, Beijin, China Method: To retrospect and analyse 160 cases (82 children as an observation group and 78 as a control Objective: study the role of hyperbaric oxygen therapy to group). For the observation group, use the hyperbaric the resumption of mycoplasma encephalitis patients. oxygen action and conventional therapy, at the same time; Contents: Female, 5-year-old. She came to hospital exert the holistic nursing of care, such as mental nursing. because of fever one day, six hours unclear sense. At the same time, provide the knowledge about the Admission diagnosed as mycoplasma encephalitis hyperbaric oxygen action for the children and the parents merger demyelinating; mycoplasma pneumonia, bacterial in order to get their coordination. Result: The effective pneumonia, septic shock. She was given such treatment hyperbaric oxygen action and nursing care made the as expansion, down intracranial pressure, curative ratio of the observation group outclass that of the anti-mycoplasma, antibiotic, nutritional support to brain control group (P<0.005). Conclusion: Treating cells, etc. After admission 20 days, pulmonary craniocerebral injury of children with hyperbaric oxygen inflammation was absorped, and she has a clear action could get better effect. While at the same time, the awareness, but not audible, remaining urine incontinence. effective nursing care is indispensable. Right leg muscle strength Class II, left leg muscle strength at class I. Review skull MRI: a little bleeding edge of the bilateral frontal lobe; bilateral subthalamic a small amount of bleeding. At that time hyperbaric oxygen therapy K03 started. Methods: GY3200 oxygen chamber of Oxygen Brain hippocampus cellular apoptosis and expression Co. Ltd. in Yantai Hongyuan, the pressure for the of bcl-2, Bax, caspase3 protein following acute treatment of 2.0 ATA, each 1 days, a total of 30 times. carbon monoxide poisoning Results: Ater 10 treatments, coordination of activities was Fujia Liu 1, Chujin Gao 1, Chengqing Xia 2, Hongying Zhao 2, improved and she,s hands shake reduction. Ater 20 Man Qi 2 treatments, she can stand and walk. Treatments of 30, 1. Hyperbaric Oxygen Department, Beijing Chaoyang she can speak simple words. Review skull MRI: brain Hospital, Beijing, China lesions were disappeared, bleeding was absorped. 2. Pathological Department, Beijing Chaoyang Hospital, Conclusion: Hyperbaric oxygen therapy plays good on Beijing, China mycoplasma encephalitis. Perhaps we should start a earlyer application of high-pressure oxygen treatment to mycoplasma encephalitis patiant just when the disease is ischemia. From 24h to 5d after MCAO HBO group under control. infarction volume didn’t change, but that of control group decreased. (3)NF-KB DNA binding activity in HBO group (1.66±1.15) at 5d after MCAO was significant lower than control group (7.28±3.10) (P<0.05). But at 3h, 24h after K05 MCAO there was no significant difference in NF-KB Interfering effect of hyperbaric oxygen and Ozagrel activity between two groups. And there was no significant Sodium on progressive stroke difference in NF-KB DNA binding activity between Liu Xinying ischemic ipsilateral cortex and contralateral cortex. Jigang General Hospital, Jinan, China Conclusions : (1) Hyperbaric oxygen could protect ischemic brain tissue at 24h after MCAO surgery, but after Objective: To approach the therapeutic effect and the 24h HBOT should be used carefully ,because it might safety of hyperbaric oxygen and Ozagrel Sodium on damage brain tissue. (2) Hyperbaric oxygen could progressive ischemic stroke. Methods: Patients with decrease NF-KB DNA binding activity of ischemic progressive ischemic stroke were treated with Ozagrel ipsilateral cortex. Sodium in the control group, while in the trial group with hyperbaric oxygen and Ozagrel Sodium. We judged the clinical nervous function and defect degree before and after 7d, 14d, 28d treatment according to the nervous K07 defect standard (NDS) and the changes of hemorrheology. Fracture treatment by hyperbaric oxygenation Results: There was a significant difference in general Yi Liu, Cao Cui effective rate and significant effectiverate between the trial Taihe Hospital Affiliated to Yunyang Medical College, group and the control group (P<0.05), and in all laboratory Hubei, China indexes there was also an apparent effects. Conclusion: The therapeutic effects of hyperbaric oxygen and Ozagrel Objective: To investigate the curative effect of Sodium is satisfactory and safe. hyperbaric oxygenation in patients with recent fracture. Methods: 80 cases were divided into hyperbaric-oxygenation group and the control group; in the hyperbaric oxygenation group, under 0.2 MPa K06 pressure, patients inhaled pure oxygen for 60 minutes Effects of large doses of hyperbaric oxygen on NF-KB each day, and between10 times and 60 times. Results: DNA binding activity of brain tissue on super-early there was great difference between two groups(P<0.05). stage of acute permanent MCAO in rats Conclusion: Hyperbaric oxygenation can promote the Yaling Liu, Qiuhong Yu, Chunjuan Wang, Lianbi Xue healing of fracture. Department of Hyperbaric Oxygenation, Beijing Tiantan Hospital, Beijing, China

Objective: We investigated the effects of large doses of K08 hyperbaric oxygen (HBO) on acute permanent focal Effects of supplementing Qi and activating blood cerebral ischemia in rats. And we examined if hyperbaric circulation and hyperbaric oxygenation on pia mater oxygen treatment (HBOT) could influence NF-KB DNA microcirculation in gerbil after cerebral ischemic binding activity of peri-infarction region of brain tissue. reperfusion injury Methods: Seventy rats underwent MCAO operation and Guoping Yu, Shilong Lin, Min Fang were randomized to hyperbaric oxygen treatment group, Shangahi Kaiyuan Orthopaedics Hospital, Shanghai, control group, sham group. Hyperbaric oxygen treatment China, began 5h after surgery. HBOT was delivered at a dose of two atmospheres absolute (ATA) for 1 hour ten times four Objective: To explore the efficacy of Chinese drugs with day. Control animals were exposed to air at ambient room the function of supplementing Qi and activating blood pressure. Other rats underwent sham circulation and hyperbaric oxygen (HBO) on the pia mater surgery. Neurological outcome was measured 3h, 24h, 2d, microcirculation in gerbil after cerebral ischemia / 3d, 5d after surgery using Garcia scores. TTC reperfusion injury. Methods: Forty － four Mongolian pathological staining and Image J software were used to gerbils underwent following operations: A cranial window determine infarction volume at the point of 3h, 24h, and 5d was created in the left parietal bone and covered with after surgery. And we investigated NF-KB DNA binding absorbent cotton soaked with artificial cerebro-soinal fluid. activity of brain tissue 3h, 24h, 5d after surgery using The bilateral common carotid arteries were squeezed by electrophoretic mobility shift assays (EMSA). Results : clamps for 30 minutes and then the clamps were (1)Garcia scores of HBO group(3.64±0.18) at the point of 24h after MCAO were significantly higher than control loosened so as to cause reperfusion ．The gerbils were group (3.21±0.12) (P<0.05). There was no significant equally divided into four group at random: drug group difference in Garcia scores between HBO group and (compound solution including Astragalus root, Chinese control group 3h and 5d after MCAO. (2) At 3h after angelica, and red sage root was infused intraperitoneally), cerebral ischemia there was no infarction region. There HBO group (exposed to 0.25MPa hyperbaric oxygen for was no significant difference in infarction volume between 60 min) ,combined treatment group (both the two above HBO group and control group at 24h after cerebal methods were used) ,and control group (normal saline was infused intraperitoneally), LMB-1 laser microcirculatory mater and JI-200 laser microcirculatory moving analytical meter were used to observe the changes of pia mater microcirculation through the cranial K10 windows before cerebral ischemia. Results: After the Cases reports for conventional hyperbaric oxygen common carotid arteries were squeezed, the treatment on altitude decompression sickness microvessels contracted obviously, microthrombus were Guangsen LIU, Shuhui ZHANG seen, and blood flow rate of the arteriole decreased to The Centre of HBOT in 458th Hospital of PLA, 0.45mm/s, and the blood flow rate of the venule Guangzhou, China decreased to 0.38mm/s.It was observed that in the drup group, HBO group, and combined treatment group the Objective: The purpose of this study was to evaluate microthrombi in pai mater were loosened gradually, and clinical effects of conventional hyperbaric oxygen exudation around microvessel was gradually entered treatment on 17 cases with altitude decompression absorption up to extinction. The arteriole blood flow rate sickness (DCI). Methods: 17 male patients (age: 25-45yr) was 1.46-1.56mm/s, the venule blood flow rate was with altitude decompression sickness were received by 1.27-1.44mm/s,significantly higher than those of the conventional HBO in single chamber filled with pure traditional. Conclusion: Chinese drugs with the function oxygen. The high altitude presented DCI occurred from of supplementing Qi and activating blood circulation by 7500m to 14000m because of chamber windows breaking playing a role in improving microcirculation. Their effects abruptly. The study population consisted of experienced are closely related to acceleration of the blood rate, pilots with the flight time from 400 to 4500 hrs. All of them regulation of the caliber of the arterioles and the venules, had different symptoms respectively, i.e. memory loss, and improvement of cerebral blood flow except oxygen hearing loss, confusion, blurry vision, headache, fatigue, content increased. joint ache. Some symptoms lasted 3-23 days after landing. All of them had received 1-3 sessions HBO (0.295MPa, 90min, one session per day) in first 3 days, and then 1 session HBO (0.20 MPa, 80min). Results: All symptoms K09 of them were completely relieved after 1-3 sessions with The effects of hyperbaric oxygenation on the HBO. The all examinations for the study population were treatment of spinal cord injury normal as before after 20 sessions HBO. 16 of 17 pilots, Zhicai Li, Yiping Song, Xiaoping Mu except 1, continued high altitude flight as before. No. 97 hospital, Xuzhou, Jiangsu, China Conclusion: In this study population, symptoms with altitude DCI would be relieved by conventional HBO. Aim: To observe the effects of hyperbaric oxygenation on the function restoration in patients with spinal cord injury. Methods: All patients with spinal cord injury were confirmed with CT and/or MRI. 126 patients enrolled after K11 January 1986 were assigned to treatment group (T group, Curative effect of hyperbaric oxygenation on with hyperbaric oxygenation therapy) and 56 patients peripheral neuropathy in type II diabetics enrolled before December 1986 were assigned to Control Jiaxiang Bai, Yanxin Liu group (C group, without hyperbaric oxygenation therapy). Tianjin Hospital, Tianjin, China There was no significant difference between two groups on the gender, age, course and injured position. Patients Objective: To observe the curative effect of hyperbaric in two groups were operated and administrated with oxygenation (HBO) on peripheral neuropathy in type II dehydrant, anti-inflammation, and drugs to against the diabetics. Methods: 76 diabetics were divided into 2 oxygen free radicals. The puncture and other physical groups equally. Diabetic diet, hypoglycemic agents, treatments were given to all patients, but only the patients vitamin B 1 20mgtid Oral treatments were used in control in T group were treated with hyperbaric oxygenation. The group. HBO combined with the above treatments were hyperbaric oxygen chamber was pressurized to 0.2Mpa used in HBO group. Patients stayed in 2.5 ATA HBO and maintain at this level. The patients in T group were chamber, wearing masks and inhaling pure oxygen for 20 given oxygen with mask according to the protocol min 4 (of pure oxygen absorption 80 minutes), with 5 min schedule of 30min×2+10min, and total time was 120min. interval. Once a day for 10 times as a course, altogether 4 A patient would need 2-4 periods of treatment dependent courses for each patient, breaks 3-5 days. on the severity of injury and 10 times as one period of Electromyography (EMG) was measured before treatment treatment. The relationship between efficiency and the and two months after the HBO treatment done. Results: beginning time of hyperbaric oxygenation therapy was In HBO group, the clinical symptoms improved observed. Results: The rate of efficiency and significantly or disappear, the motor nerve conduction improvement was 61.9% and 31.0% respectively in T velocity (MNCV) and sensory nerve conduction velocity group, which was significantly more than 23.2% and (SNCV) of EMG were increased significantly after the 37.5% in C group (p<0.05). Conclusion: Hyperbaric treatment than before (P < 0.01), while clinical symptoms oxygenation could promote the function restoration of of the control group had no significant improvement, in injured spinal cord, especially for the patents with partly EMG Figure part (upper) a slight improvement in nerve spinal cord injury. The patients with spinal cord injury conduction velocity, and no other significant changes. should be treated with hyperbaric oxygenation as earlier Conclusion: HBO can effectively improve diabetic as possible. peripheral neuropathy, worthy of promotion. were significantly higher than in control group (P<0.05). Conclusion: HBO may help to recover injured patients’ language abilities. K12 Effect of hyperbaric oxygen treatment on hydrocephalus in animal with acute ischemic cerebral injury K14 Shilong Lin 1, Jingchang Liu 2, Peizhu Xin 2, Guooping Yu 1 Hyperbaric oxygen treatment of 28 cases of children 1.Shanghai Kaiyuan Orthopedic Hospital , Shanghai, viral encephalitis China Xiuqing Liang, Xiaole Wang, Xiaohong Wang 2.Naval Medical Research Institute of PLA, Shanghai, Shanxi Province Maternal and Children Care Hospital, China Xi’an, China

Objective: To approach effect of hyperbaric oxygen Viral encephalitis in pediatric clinical is common, and (HBO) treatment on hydrocephalus in animals with acute major features of fever, headache, convulsions, cerebral ischemic injury. Methods: 60 gerbils were disturbance of consciousness, abnormal behavior, high divided into the cerebral ischemic group, the hyperbaric intracranial pressure. Laboratory abnormalities can be oxygen exposure group and the control group. These found in cerebrospinal fluid, the virus culture positive, animals were anesthesia with 20% urethane (1.2g/kg.BW) cranial CT and EEG abnormalities, etc. In 1991 to 2007, or 20% pentobarbital sodium (40mg/kg B.W.). Acute admitted in October to 11-year-old children with 28 cases cerebral ischemic injury was resulted from occluding both of encephalopathy with anticonvulsant, decrease carotid for 30min. Animals were exposed at 0.25MPa intracranial pressure and the anti-virus treatment, HBO for 60min after cerebral ischemic injury in the HBO especially add to hyperbaric oxygen therapy. All of the group. Cerebral ischemic injury group was exposed to patients were given oxygen chamber treatment. 0.08 Mpa N2-O2 with O 28%-N292% at 0.25MPa for 60min. The pressure lasts two hours, once a day for 10 consecutive control group animals were exposed to normal pressure treatment every week treatment interval. course of for 60min in the chamber. Blood flow dynamic effect in treatment is from 2 to 8. The results showed that cured 16 microcirculation, morphosis in micro-vascular endothelial cases, eliminate clinical symptoms, EEG, cerebrospinal cell and cerebral nerve cell detected. Result: after 30min fluid and cranial CT returned to normal. 9 cases turn for to block up carotid, cerebral nerve cell injury occurred, the better, eliminate clinical symptoms, cerebrospinal fluid part nerve cell nucleus was vacuole, denaturalization and of normal, but abnormal EEG and cranial CT abnormal; swell. Cerebral tissue was edema. Endothelial cell invalid three cases, No clinical symptoms improve, structure and function changed, and edema formed continuing convulsions, abnormal EEG and cranial CT, around micro-vessel. After HBO exposure for 60min, brain conversion is shown. Hyperbaric Oxygen Therapy cerebral nerve cell swell improved in animal with cerebral encephalopathy is mainly to facilitate the brain tissue injury. Only spot exudation occurred around micro-vessel. collateral circulation, reducing hypoxic brain damage, Endothelial cell injury obviously decreased. increasing reticular activating system’s oxygen, to speed Hydrocephalus entered absorption. After HBO exposure up the unconscious child awake. Compared with simple at 0.25MPa for 60min, blood perfusion flow increased dehydration, anti-virus and anticonvulsant treatment, than the control group in animals with cerebral ischemic hyperbaric oxygen is significantly. In addition, we also injury (P<0.01). Conclusion (1) Acute ischemic cerebral found that the severe convulsions, plus saiga horn oral injury may cause hydrocephalus resulted in cerebral solution, which can effectively reduce the seizures nerve cell, endothelial cell and other cerebral tissues occurred and prevent temperature rebound. injury. (2) HBO has therapeutical effect by reducing injury in nerve cell and micro-vessels endothelial cell in animals.

K15 Study on prevention of the delayed neuropsychologic K13 sequelae after acute carbon monoxide poisoning in Interfering effect of hyperbaric oxygen on alogia in hyperbaric oxygen treatment acute cerebral injury Li Zengmin Lin Ying Hyperbaric oxygen section of City center hospital, Xian, Hyperbaric Oxygen Derpartment of the Affiliated Zhong China Shan Hospital of Xiamen University, Xiamen, China Objective: Research how lower the delayed Objective: To study the interfering effect of hyperbaric neuropsychologic sequelae of outbreak rate in the oxygen on aphasia people with acute cerebral injury. hyperbaric oxygen treatment the acute the carbon Methods: 120 cerebral injury people were divided into the monoxide poisoning of process. Methods: Through hyperbaric oxygen (HBO) exposure group and the collator review cultural heritage and r former clinical data of group. Each group had 60 people. HBO group were analysis, take a series valid measure, to hyperbaric accepted HBO treatment. Results: After 12-24 times or oxygen treatment the project did correspond of more HBO treatment, it showed that the blocked vessels improvement. Results: Make acute carbon monoxide were repassed or injured cerebral zones were rebuilt from poisoning the delayed neuropsychologic sequelae of CT or MRI impression. Injured patients’ language abilities outbreak rate reduced obviously. Conclusion: Combine immediate, enough of hyperbaric oxygen treatment and The affection research of micronucleus frequency in reasonable of comprehensive treatment are the best pregnant mice bone marrow erythrocyte treated by treatment project. hyperbaric oxygen Li Zhang Second hospital attached to Kunming medical collage, Kunming, China K16 Purpose judged curative effect of wean cerebral palsy Objective: This paper has evaluated the frequency of by hyperbaric oxygen treatment. micronucleus in the pregnant mice bone marrow Zuomin Li, Wenying Huang erythrocyte treated by hyperbaric oxygen. Methods: We First Renmin Hospital of Chenzhou, Huhan, China have treated the pregnant mice by hyperbaric oxygen one time every day, and examined the frequency of Purpose: Judged curative effect of wean cerebral palsy micronucleus in the pregnant mice bone marrow by hyperbaric oxygen treatment. Method: 75 case erythrocyte, in order to research the affection of the cerebral palsys were divided into two groups: treatment genetic materials in pregnant mice treated by hyperbaric group 38 case and antitheses 37 case. Compared the two oxygen. Results: The frequency of micronucleus in the groups with symptom, system by crued fore-and-aft; and treated group is significant different compared with the compared the conclusion based on CT or MIR result. control group. Conclusion: Perhaps excessive Result: Myospasm, involuntary movements, hyperbaric oxygen can make damage of the genetic incoordination, intellectuality, speech function improved in; material in the pregnant mice to a certain degree. epilepsy spasm `times decreased; it is more difference compare with antitheses of HBOT group’s case (p<0.05). Conclusion: HBOT is credibility and affirm method than simplicity medication in wean cerebral palsy treatment. K19 The penis plants again match carried on the hyperbaric oxygenation treatment to cure 1 Li Zengmin K17 Central Hospital XIAN, Xi’an, China Monitoring bacteria status in the water and container of humidifying in single-chamber with pure oxygen Objective: The penis plants again behind for the sake of Chaoqun TU, Xiaomei XIE, Xiufang MEI, Meilian LAN, Sh anti- infect and improve the blood oxygen a supply ,the uliang LI match carried on the hyperbaric oxygenation treatment. The Shenzhen Peoples Hospital, Shenzhen, China Methods: The treatment adopts large air to add to press cabin ;normal regulations treatment project. Results and Objective: To observe the bacteria status in the water Conclusion: Thus for again the surgical operation and container of humidifying in the single-chamber with provided a guarantee successfully. pure oxygen in order to find right method to prevent from second contamination from humidifying water in the chamber. Methods : 1.The water in the container of chamber and from spraying nozzle in the chamber was K20 cultured for consecutive 5 days. The water was divided Effect of Hyperbaric Oxygen Treatment on learning into three groups, non-heating water, drinking water and and memory aspects of transient global cerebral distilled waterdistilled water, was changed on alternate 3 ischemia in rats days145±26 cfu/m 3)found at the two sites from Yajuan Li 1, Chunjin Gao 1, Yu Zeng 2, Zhipeng Xu 1, Hui W non-heating water appeared on third days. The TBC from ang 1 drinking water and distilled water were respectively 1. Capital Medical University affiliated Beijing Chaoyang 472±69 cfu/m 3, 357±91cfu/m 3on forth days. 2. No bacteria Hospital, Beijing, China appeared at the above sites from the water of drinking and 2. Sichuan Provincial Peoples Hospital, Chengdu, China distilled water on the third day when water had been changed on alternate 3 days. Conclusion: 1.The distilled Objective: To observe the effect of hyperbaric oxygen water should be best liquid fit to humidify in the treatment (HBOT) on rat’s abilities of leaning and memory single-chamber with pure oxygen. 2. It should be to clean in the transient global cerebral ischemic model and to the container and distilled water should be changed at explore the mechanism of hyperbaric oxygen treatment least on alternate days . . and was cultured at the same on recognition impairment after cerebral ischemic method on third day. Results: 1. No bacteria of three kind diseases. Methods: 46 male Sprague-Dawley rats were of water were found at the two sites. The bacteria randomly divided into 4 different groups: blank group (average total bacterial count, TBC. 2. The water divided (n=10), sham-operation group (n=10), cerebral-ischemia into two groups, drinking water and group (n=9), HBO group (n=9). According to Pulsinelli’s 4-vessel occlusion method to establish transient global cerebral ischemic model on the cerebral ischemia and HBO group. The sham-operation group didn’t block the K18 vertebral artery and the carotid artery. The blank group has no operation, only take part in Morris water-maze. HBOT was performed to rats in HBO group after the second operation for 7days, one time for one day, then we give all the rats a test of Morris water-maze. We observed the time of escape latent and the last day when move off the platform, the time of rats through the platform .The K22 average time of every day and every group was compared. The hyperbaric oxygen therapy for spinal cord injury The SPSS11.5 software was used for statistical analysis, caused by acute cervical disc herniation rank sum test was used in the 1 st -5th ,analysis of variance Shaohua Liang, Xiaoju Tang, Ruzhuan Liu and Newman-Keuls test were used in the last day, p<0.05 The Ruikang Hospital Affiliated to Guangxi University of has statistical significance. Results: 1. The escape latent Traditional Chinese Medicine, Nanning, China comparison:the HBO group is shorter than the cerebral ischemia in the 3rd -5th day (p<0.01 or p<0.05), the Objective: To evaluate the clinical effects of the difference has statistical significance; the HBO group and hyperbaric oxygen therapy for spinal cord injury caused sham operated group in the 1st -5th day has no difference; by acute cervical disc herniation. Methods: To the sham operated group is shorter than the cerebral retrospectively analysize the clinical data of spinal cord ischemia group in the 3rd and 5th days (p<0.01 or p<0.05), injury caused by acute cervical disc herniation of 45 the difference has statistical significance; the blank group cases ,which was treated with the hyperbaric oxygen is shorter than the sham operated group in the 2nd- 5th therapy after surgery. To evaluate the clinical effects, day (p<0.01 or p<0.05), the difference has statistical based on the Frankel grade and the JOA score significance.2. The times of through platform's improvement rate of preoperative and postoperative comparison: the HBO group is larger than the cerebral situation. Result: the Frankel grade of patients improves ischemia group in the last day (p<0.05), the difference has 1-4 level. The time of follow-up is from 6 months to 2 statistical significance; the HBO group and the sham years, the average time is 10 months. the JOA score operated group has no difference; the sham operated improvement rate: excellent: 23 cases(51.1%), good: 18 group is larger than the cerebral ischemia group(p<0.01), cases(40.0%), average:3 cases(6.7%), poor:1 case (2%), the difference has statistical significance; the blank group The good-to-excellent rate is 91.1%. Conclusion: The is larger than the sham operated group (p<0.01), the hyperbaric oxygen therapy for spinal cord injury caused difference has statistical significance. Conclusions: 1. by acute cervical disc herniation, is the effective therapy The learning and memory abilities of transient global of recovery of spinal function. cerebral ischemia in rats are decline. 2. HBOT can improve the impairment of learning and memory after transient global cerebral ischemia in rats. K23 Clinical studies on sudden deafness via hyperbaric oxygen therapy K21 Ying Mei 1, Chaofeng Xie 2 Effect of High pressure oxygen (HBO) for Treating 1. HBO Department, People’s Hospital of Guangdong Neonates with Bypoxic ischemic encephal opa-thy of Province, Guangzhou, China the newbom (HIE) 2. E.N.T Department, Shantou Second People’s Hospital, Yuxian Li, Mei Lin Shantou, China Youjiang Medical College for Nationalities of Affiliated Hospital, Guangxi, China The therapeutic effect on the clinical efficacy of hyperbaric oxygen treatment on sudden deafness was discussed in Objective: To study the effect of HBO for treating this study. The method is that 300 cases of sudden deaf neonates with HIE. Methods: 160 cases hospitalized patients were randomly divided into two groups: drug in our department who were diagnosed with HIE treatment group (group A),and the hyperbaric oxygen according to the diagnosis stander. They wear divided treatment with drug group(HBO group, or group B). the into treatment group and control group. There was no two groups with drug treatment made the same effect, but significant difference between the two groups of the in addition, HBO group was given the treatment of HBO, clinical classification, lesion degree and days (p>0.05). that is, under the following conditions: the Control group was given regular treatment, while pressure-0.22Mpa,oxygen absorption-30min*2,the treatment group was added HBO after the life sings interval-5min,one treatment for one day,12 days for a was stable. The treating pressure adjusted to the days course. Generally, the effect comparisons were taken in old.10 days were a course .They were assessed after one to three courses. Overall, the effect of the the treatment .The results were compared. Results: combinative group is better than the drug group, and the There was difference between the two groups of the variation is obvious (P<0.05). And the conclusion is: the effective rate. Effect of treatment group was better than treatment on sudden deafness with hyperbaric oxygen that of control group. All cases were followed up for and drug is obvious, and should be advocated broadly in half a year. Their neural development and the the clinical application. Keywords: sudden deafness intelligence were observed .There was statistic hyperbaric oxygen combination treatment difference between two groups adverise prognosis rate. Conclusion: patients with HIE should be given HBO as soon as possible after stable disease .It can improve the clinical symptom and the cure rate K24 shorten ,the course of disease. To treat hard facial nerve paralysis with hyper--baric different treatment methods were centrifugated to obtain oxygen (HBO)combined with electric acupuncture the supernatant, and CGRP and ET levels were Shunyong Li, Aixiang Du, Guicui Wang measured by radioimmunology method. Results: WeiHai Municipal Hospital, Weihai, China Compared with control group, CGRP level shows decreasing tendency with time extension, without Objective: To compare the curative effect of HBO in significant difference. The hyperbaric oxygen treatment patients with hard facial nerve paralysis with of HBO has no effect on CGRP level, but hypobaric hypoxia combined with electric puncture. Methods: 37 cases of pretreatment can make CGRP level decrease more patients were treated simply with HBO once per day,the obviously, without significant difference. ET level shows duration of one period of treatment lasted for 10 days. increasing tendency with time extension, but without 39cases of patients were treated with HBO and electric significant difference. However, EF level after hyperbaric acupuncture for five period of treatment (To acupuncture oxygen and hypobaric hypoxia pretreatment shows the routine facial nerve paralysis points once per day, ten significant increasing, P<0.05. Conclusion: The times of acupuncture made up of one period of treatment). hyperbaric oxygen and hypobaric hypoxia training mainly Results: O f the HBO group, 12 cases (32.43%) were influences ET level change in rats, and hyperbaric cured, 18cases (48.65%) were relieved, 7cases (18.92%) pretreatment might enhance body adaptive capacity on were no curative effect. Of the HBO combined with mountain. electric acupuncture group, 24 cases (61.54%) were cured, 12 cases (30.77%) were relieved, 3cases (7.69%) were no curative effect. Conclusion: The curative effect of using HBO combined with electric acupuncture in K26 treating patients with hard facial nerve paralysis is Observation of hyperbaric oxygenation theraphy on significantly better than that of using HBO simply. patients with insomnia Yulin Nie, Kunru Zhou Buji people’s hospital, Shenzhen, China

K25 0bjective: To observe the effects of hyperbaric Effect of early intervention of hyperbaric oxygen and oxygenation on sleep quality of the patients with hypobaria on CGRP and ET level in rats under insomnia. Methods: Ninteen patients with insomnia were hypobaric environment treated with hyperbaric oxygenation, and observe the Shuyi Pan 1, Xiaowen Pan 1, Xiangen Meng 1, effects of hyperbaric oxygenation on sleep quality and Mingxin Li 1, Yu Zhang 1, Chen Yang 1, Baoguo Li 2 simultaneous phenomenon. Results: 5 cases totally 1.Navy General Hospital, Beijing, China recovered, 12 cases had obvious effect, and 2 cases had 2.Shi jitan Hospital, Beijing, China no effect. Conclusion: Sleep quality and simultaneous phenomenon of the patients with insomnia can be Objective: To explore the effect of early intervention of improved with hyperbaric oxygenation. hyperbaric oxygen and low atmospheric pressure on CGRP and ET level under hypobaric environment, and to compare the effects of two methods. Methods: The Wistar rats were randomly divided into 6 groups: (1) K27 Control group, (2) 3800m (2h) group, (3) 3800m (3d) Use hyperbaric oxygen therapy to treat radiating group, (4) 3800m (7d) group, (5) Hyperbaric oxygen injury intervention group, (6) Hypobaric environment Jun Qu, Jun Meng intervention group. Firstly, the rats in the second, third and HuanHu Hospital, Tianjin, China fourth groups were placed in low pressure oxygen chamber, and the pressure in chamber was decreased at We all know that radiating injury often can be found in equal speed from 0.1mpa to 0.062mpa from the sea level, radiation therapy of malignancy tumour. Rradiating injury to simulate hypobaric environment on mountain at 3800m has a strong impact on patient’s life quality. Hhyperbaric above sea level. At 2h, 3d and 7d time points, the rats oxygen therapy (HBOT) which known as a useful way to were decapitated to obtain blood for later examination; some disease had application in curing the patients. But Secondly, the rats in the fifth group received hyperbaric there were few reports about radiating injury now. This oxygen treatment (0.2mpa, once per day, oxygen review was discuss about the mechanism of how radiating inhalation for 60 minutes, successively 5 days). At the injury happened and the mechanism of HBOT to treated it. sixth day, the rats were placed in low pressure oxygen HBOT also can be consider as a new way to cure chamber, and the chamber was decreased at equal speed radiating injury. to 0.062mpa. At 2h time point, the rats were decapitated to obtain blood for later examination; thirdly, the rats in the sixth group received hypobaric hypoxia treatment (0.062mpa, once per day, 2h once, for successively 5 K28 days). At the sixth day, the rats were placed in low The diagnosis and treatment experience of hyperbaric pressure oxygen chamber, and the chamber was oxygen (HBO) comprehensive treatment for decreased at equal speed to 0.062mpa. At 2h time point, persistent vegetative state (pvs) the rats were decapitated to obtain blood for later Juan Meng, Xiaowen Pan, Yu Zhang, Huijun Hu, Yan Lu, examination; the blood samples obtained from above Shuyi Pan, Xiangen Meng Hyperbaric oxygen center in the General Naval Hospital, methods of S group were similar to that of C group except Beijing, China no MCAO. The change of behavior, somatosensory evoked potential (SEP) and infarct volume in these rabbits Objective: to discuss and study the treatment effect of was observed respectively for 10 days and 20 days after hyperbaric oxygen (HBO)comprehensive treatment for MCAO. Results: Ten days after MCAO, the mean score persistent vegetative state(pvs) and the importance of of behavior of I, D, C and S groups was respectively advanced radiological technology for pvs. Methods: for 8.6±1.2, 5.8±0.8, 5.6±1.6 and 12±0.0. The 280 pvs patients with HBO cooperating with blood neurobehavioral impairment in I group relieved magnetization therapy and drugs treatment and with significantly compared with that of D and C groups, (p 3, advanced radiological technology. Results: this group of 214±19.7 mm 3, 229.4±25.5 mm 3 and 0 mm 3 for 10 days patients showed that the diagnosis coincidence rate after MCAO, the infarct volume of I group was significantly reached 100%, the curative ratio (85 cases of clear smaller than that of D and C groups (p 3, 203.4±12.7mm 3, consciousness, normal intelligence and self-care of living) 221.4±20.3mm 3 and 0mm 3.The infarct volume of I group is 30.4%, the improvement rate (48 cases of clear was significantly smaller than that of D and C groups consciousness, basal self-care of living and basal normal (p<0.01). Conclusion: Early applications of HBO and intelligence) is 52.8%, 15.2% (43 cases) of patients have repeated administration of HBO for 20 days have not left PVS, 98.4% of patients have gained improvement significant effects on the change of behavior, of general condition (276 cases of control of infection, somatosensory evoked potential (SEP) and infarct volume correction of complication, pulling out of urethral catheter, of rabbits which suffering from MCAO. nasal feeding tube and tracheal tube after tracheotomy and improvement of nutrition status), 1.6% of patients (4 cases) died. The major abnormal signs in cerebral CT of this 280 cases PVS patients contained: cerebral edema, K30 malacia focus, focus of infarct, subdural effusion, The therapeutic efficacy of hyperbaric oxygen obstructive hydrocephalus, bilateral hypophloeodal diffuse treatment for brain infraction was influenced by the white matter demyelination change, bilateral cerebrum onset time: micro dialysis study in rabbits atrophy, thinner cortex, ventricles of brain enlarging, Zhaopang Lai, Fei Li, Zhi Liu, Hua Feng broaden of sulcus and schizencephaly, malacia focus of Department of Neurosurgery, Southwest Hospital, Third basal nuclei and globus pallidus in the damage region. 76 Military Medical University, Chengdu, China cases of MRI examination could also show the atrophy of patients’ mesencephalon and brain stem except for the Objectives: To observe if the therapeutic efficacy of signs before. Cerebral SPECT: 32 cases showed hyperbaric oxygen treatment for brain infraction was decreasing of bloodstream perfusion in bilateral or influenced by the onset time after permanent middle unilateral cerebral cortex, cerebellum, unilateral or cerebral artery occlusion (MCAO) in rabbits. Methods: 36 bilateral frontal lobe, temporal lobe and occipital lobe. The male rabbits were randomly divided into simple MCAO radiological imaging of mostly patients improved along group (n=12) , MCAO+ HBO group (100% O2, 2.5 ATA, with improvement of state of illness. Conclusion: the 1h / d, from 1 d after MCAO, n=12) and MCAO ＋DHBO HBO comprehensive treatment scheme was reasonable, group (100% O2, 2.5 ATA, 1h / d, from 7 d after MCAO, advanced, practical and innovative. The curative effect n=12). Behaviors and volumes of infarction were was good: Management to complications was timely and observed, microdialysis was applied to monitor the effective. Advanced radiological technology cerebral CT concentrations of glucose, lactate, pyruvate and and MRI are not only helpful to diagnosis, but also helpful glutamate around the infracted zone at 1,3 ,10and 30 d to guide treatment and judge prognosis. The cerebral after permanent MCAO. Results: Behaviors’ score and SPECT imaging could be complementary with CT and volumes of infarction were lower in MCAO+ HBO group MRI. It also could act as an index of HBO comprehensive than the others (P<0.01). The lactate and pyruvate ratio treatment for PVS and prognosis determination. increased after MCAO in three groups, but they were

lower in the MCAO+ HBO group than in the others at Day

1 and Day 3(P<0.05). The glutamate concentration

increased after MCAO, peaking at 10 d, but at day 1 and K29 day 3 the glutamate concentration was lower in the Therapeutic efficacy of hyperbaric oxygen on middle MCAO+ HBO group (P<0.05). Conclusions: Hyperbaric Jiexiang Pan, Weihua Chu, Fei Li, Hua Feng oxygen treatment could protect the brain from infarction Department of Neurosurgery, Southwest Hospital, Third through improving the energy metabolism and decreasing Military Medical University, Chengdu, China the excitatory amino acids disorders around the infracted

zone after MCAO in rabbits. In order to improve the Objective: To evaluate the efficacy of hyperbaric oxygen therapeutic efficacy of hyperbaric oxygen, it should be (HBO) treatment on model of right middle cerebral artery performed as early as possible. occlusion (MCAO) in rabbits. Methods: Forty healthy rabbits were suffered MCAO using electragulation and were randomly divided into 4 groups. The rabbits in I group were treated with HBO immediately for 20 days K31 after MCAO. The rabbits in D group were treated with HBO 7 days after MCAO for 13 days. The rabbits in C group were not treated with any therapy after MCAO. The The expression of nestin was elevated by hyperbaric energy metabolism and decreasing the excitatory amino oxygen treatment in a model of right middle cerebral acids disorders around the infracted zone after MCAO in artery occlusion in rabbits rabbits . Jiexiang Pan, Weihua Chu, Fei Li, Hua Feng Department of Neurosurgery, Southwest Hospital, Third Millitary Medical University, Chengdu, China K33 Objectives: To evaluate the efficacy of hyperbaric Use hypenbaric oxygen( HBO) to treat 8 patients of oxygen (HBO) treatment on the expression of Nestin in a viral meningitis model of right middle cerebral artery occlusion (MCAO) in Jun Meng, Jun Qu rabbits. Methods: 30 healthy rabbits were randomly HuanHu Hospital, Tianjin, China divided into 3 groups: The rabbits in MCAO+HBO group were treated with HBO immediately for 20 days after MCAO, The rabbits in MCAO group were not treated with Objective: Use hyperbaric oxygen (HBO) to treat viral any therapy after MCAO, The methods of control was meningitis. Method: Use hyperbaric oxygen (HBO) to similar to that of MCAO group except no MCAO. treat 7 patients of viral meningitis and we will observe the Behaviors and volumes of infarction were observed, and change of hydrocephalus in medical image and the the expression of Nestin was measure by recovery of consciousness, language, limb strength. immunocytochemistry at 10 and 20 days after MCAO. Result: All the patients have a good curative effect in Results: Behaviors’ score and volumes of infarction were what sides we observed. Conclusion: Use hyperbaric better in MCAO+ HBO group than the MCAO group oxygen therapy to treat viral meningitis at the end of the (P<0.01).10 days after MCAO, the expression of Nestin of course maybe beneficial to the recovery of the naval MCAO+ HBO group and MCAO group was respectively function. 15.88±1.2 and 6.63±1.6. 20 days after MCAO, the expression of Nestin of MCAO+ HBO group and MCAO group was respectively 20.03±1.6 and 6.82±0.8. The expression of Nestin in MCAO+HBO group relieved K34 significantly higher compared with that of MCAO group Effect Analysis of early and delayed hyperbaric (p<0.01). Conclusion: Early applications of HBO and oxygen treatment on ischemic brain injury of rabbit repeated administration of HBO for 20 days have Zhaopan Lai, Zhi Liu, Gaoyu Cui, Jiangkai Lin, significant effects on the expression of Nestin of rabbits Xianrong Wang, Hua Feng which suffering from MCAO. Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chengdu, China

Objectives: To investigate the effect of early and delayed K32 hyperbaric oxygen (HBO) treatment on local ischemic Regional biochemical changes following ischemic brain injury in rabbit. Method: 32 rabbits were randomly brain injury in rabbits ：：： microdialysis studies and divided into 4 groups: control group(sham), middle cerebral artery obstruction (MCAO)group, MCAO+HBO effects of hyperbaric oxygen preconditioning group, and MACO+DHBO group(delayed HBO started at Chongying Deng, Fei Li, Lijun Zhang, Hui Meng, Jiangkai 7 days after MCAO). 2 atmosphere pressure HBO was Lin, Hua Feng applied for 1 h per day, and neurological score, TTC Department of Neurosurgery, Southwest Hospital, Third dyeing and pathomorphological observation were Military Medical University, Chengdu, China performed to evaluate the effect of early and delayed

HBO treatment. Result: Neuroethological abnormalities Objectives: To observe the variations of regional could be observed in all experimental groups except biochemical changes around the infracted zone after control group. 30 d after obstruction, ethological score permanent middle cerebral artery occlusion (MCAO) in showed rabbits in MCAO+HBO group was better than rabbits and evaluate the effects of hyperbaric oxygen MCAO+delayed HBO (P<0.05); TTC dyeing showed the precongitioning (HBOP). Methods: 48 male rabbits were infarct size of MCAO, MCAO+HBO and MCAO+delayed randomly divided into simple MCAO group (n=24) and HBO were 378±35.6 mm3, 59±17.2 mm3 and 139±45.8 HBOP+MCAO group (100% O2, 2.5 ATA, 1h / d, 5d, mm3, respectively, with a significant difference in n=24) . Microdialysis was applied to monitor the MCAO+HBO group compared with other two groups concentrations of glucose, lactate, pyruvate and (p<0.01). Neuron edema, degeneration and decrease in glutamate around the infracted zone at 1, 3, 10 and 20 d cell number were observed with HE staining, which was after permanent MCAO. Results: Glucose decreased at 1 more server in MCAO group and indistinct in MCAO+HBO d after MCAO in two groups, but in the preconditioning group. Conclusion: Early HBO treatment has an group glucose recovered to normal at 3 d, while in the advantage on the local ischemic brain injury of rabbit simple MCAO group it still decreased at 20 d and compared with delayed HBO. significantly lower than the left ( PP <0.05). Conclusions:

1, The variations of the components of the microdialysis interstitial fluid from the brain support the presence of

“biochemical penumbra” around the infracted zone after K35 MCAO ；2, the mechanisms of HBOP maybe improving the The effect of HBO on survival of the arterialized deep-venous flaps in a rat model Ying LONG, Junjun HE, Jiewen TAN, Xiaojun LI, K37 Yulin NIE, Zhi YI Reports of 48 cases about hyperbaric oxygen 1. The Shenzhen Peoples Hospital, Shenzhen, China approach in treating thromboangiitis obliterans 2. The Second Affiliated Hospital of Sun Yat-sen Jinjun Pang, Fengjuan Long, Guihua Pan, Yafan Li University, Guangzhou, China First Affiliated Hospital, Guangxi Medical University, 3. The Buji Peoples Hospital, Shenzhen, China China

Objectives : the effect of hyperbaric oxygen therapy (HBO) on survival of the arterialized deep-venous flaps and Objective: To explore the effects of hyperbaric oxygen on blood supply was studied in a rat model. Methods : In this thromboangiitis obliterans (TAO). Method: There are 48 study, the arterialized deep-venous flaps with pedicle cases in our study.8 cases in stage Ⅰ,18 cases in stage Ⅱ were established and anastomosed by proximal end of external iliac artery with external iliac vein of flaps on the and 22 cases in stage Ⅲ .All cases were treated by abdomen of rats. An experimental model was created in hyperbaric oxygen. Medicine was applied to severe cases retrograde blood supplied to flaps from external iliac vein We compare the effects in different stage and course of inferior and epigastric vein. The 45 of SD rats were treatment. Results: 37 cases were cured,10 cases were randomly divided into three groups. The blood supply of effective, The total effective rate was 97.9%.26 cases in flaps in Group I (Control, n=15) was anastomosed by stage Ⅰ or stage Ⅱ were completely cured, the cure rate end-to-end with arteries and venous respectively. 30 of 45 rats were anastomosed in arterialized deep-venous flaps was 100%.The cases the cure rate was only 50% in stage of rat model(ADVF). 15 rats with ADVF (GroupII, HBO) Ⅲ .36 cases accept 4 courses hyperbaric oxygen were received by HBO treatment at 0.2MPa twice daily for treatment, 33 cases were cured, the cure rate was 3 consecutive days after operation. Another 15 of 91.7%.12 cases were treated less than 4 courses; the ADVF(Group III , non-HBO) were received by cure rate was 25% . Conclusions: Hyperbaric oxygen has conventional treatment after operation. Clinical survival a notable curative effect on TAO. We can receive high assessments of flaps included perfusion monitored by cure rate if we adopt complete courses hyperbaric oxygen observation of mass species with naked eye. The survival treatment in stage Ⅰ and stage Ⅱ. rate of flaps was calculated after operation. Result: 1.The edema of flaps in ADVF was reduced by HBO . The color of flaps after HBO was red in the similar to Group I. The Flaps in Group III were going to be black red from 2. day after operation and dying on 3.day after operation. 2.The K38 survival of the flaps were respectively 60%, 66.7%, 0. Hyperbaric Oxygenation on Limb Dyskinesia after Conclusion: This study indicates that it iseffectiveness of Intervention of intracranial Aneurysm and Its Effects on ADL among 32 Cases blood circulation in this arterialized deep-venous flaps can be enhanced by retrograde arteriali- zation in this animal Yongjun Mai, Xuedong Li, Meiyin Lei, Hua Huang model. HBO could improve blood supply of the flaps with HOB center, the Fourth Affiliated Hospital, GuangXi ADVP and reduce edema after operation and increase Medical University, Liuzhou, China flap survival with ADVP. Objective: To determine the effects of early hyperbaric oxygenation (HBO) on the prognosis of motor function K36 after intervention of intracranial aneurysm. Methods: 62 Cancelling the requirement of ventilation for patients with dyskinesia after intervention of intracranial hyperbaric chamber pressurized with oxygen aneurysm were randomized into HBO treatment group and control group. Both group received the same routine Mao Fangguan th th Chinese Society of Hyperbaric Facility, Shanghai, China medicine. In addition, HBO group started HBO at 7 –10 day after intervention. Defects of nerve function and ADL Objective: When the volume fraction of oxygen of gas in were evaluated with Fugl-Meyer Scale and Barthel Scale respectively. Results : Neurofunction defects and ADL a chamber is reduced the potentiality of fire will decrease and the operational safety will increase for the hyperbaric were significantly improved 30 days after treatment in chamber pressurized with oxygen. Methods: By means of both groups. There was statistic significance compared analyses and calculations the maxima of oxygen patial with the conditions after interventions (P<0.01). The results of HBO group were much better than those of pressure ( pO 2) and carbon dioxide partial pressure ( pCO 2) in the chamber can be determined under conditions of control group. There was statistic significance between the two groups (P<0.01). Conclusion: Early HBO can nonventilation. Results: In this situation the pO 2 may be raised to 0.22 MPa, it can meet the demands of HBO improve patients’ ADL and defects of nerve functions after intracranial aneurysm intervention, which enhances conventional therapy and the maximum of pCO 2 less than 3 kPa is safe on the human body. Conclusion: It is patients’ life quality. favorable and reasonable to cancel the requirement of ventilation for a hyperbaric chamber pressurized with oxygen. K39 The EEG analysis before and after of hyperbaric function. Conclusion: the level of immune cells in the oxygen therapy in patients with cerebral infarctinon stability of patients with COPD is degraded in varying Ma YuJie degrees, the reduction of HBO treatment can significantly No.1 Hospital, Qiqihaer, China improve the stability of the cell-mediated immunity in patients with COPD function, at the same time to correct Objective: Analysis and Comparison of patients with hypoxia and CO2 retention and improve lung function in cerebral infarction in HBO treatment before and after the patients with COPD (P <0.01). EEG changes, study the prognosis of the disease. HBO treatment can reduce the incidence of meaningful consequences. Methods: HBO group in the conventional treatment at the same time increase the HBO therapy. K41 HBO use large cabin wearing oxygen masks in 30 ″×2, the Effect of hyperbaric oxygen to inflammatory cytokine middle cabin air suction, once a day, 10times for a course in patients with obstructive sleep apnea-hypopnea of treatment, 2-3 courses of treatment. Conventional syndrome treatment of the control group, using low-molecular-weight Yongxin Ma 1, Nong Yu 2, Bing Meng 2 dextran 500ml and dan senate 14 ml; 5%glucose injection 1.Weifang people’s hospital, Shandong, China 250 ml and CDP- choline 0.5g intravenous drip, once a 2.Weifang Asthma hospital, Shandong, China day, seven days for treatment, treatment 2-3 Courses. Results: HBO group EEG abnormalities before treatment Objective: To observe the effect of hyperbaric to 79 percent, 51 percent after treatment, down oxygen(HBO) to inflammatory cytokine in patients with 28%,compared before and after treatment were obstructive sleep apnea-hypopnea significantly different (P<0.05) ； The control group EEG syndrome(OSAHS). Methods: Thirty two patients with OSAHS were randomly divided into 2 groups: HBO abnormalities before and after the treatment rate of 80 therapy group and control group. The T lymphocyte percent, reduced to 66 percent, down 14 percent; no + + + + subset,CD ,CD ,CD /CD and inflammatory significant difference (P>0.05). Conclusions: HBO can 4 8 4 8 cytokine,IL-6,IL-8as well as TNF-α,were assayed by flow improve the prognosis of cerebra infarction patients, cytometry after HBO exposure. Result: The level of CD4+ whether from the clinical efficacy or EEG of recovery, the and CD4+/CD8+ ratio tended to increase after HBO effect is very obvious that the lower incidence of sequelae exposure 7 times, while significantly decreased (P <0.001) and provide a reliable basis for the treatment. So routine after HBO exposure 14 times and 21times. The level of treatment of patients with cerebral Infarction at the same inflammatory cytokine,IL-6,IL-8 and TNF-αtended to time as soon as possible with HBO treatment. decreased (P <0.005) after HBO exposure 7 times, and

significantly decreased too (P <0.001) after HBO

exposure 14 times and 21times. Conclusion: HBO

therapy can not only adjust the balance of immune, K40 + decreased the cytotoxic action of CD lymphocyte, but Influence of hyperbaric oxygen to the immune 8 also significantly decrease the level of inflammatory function of cells in the patients with chronic cytokine in patients with OSAHS. obstructive pulmonary disease

Bing Meng 1, Yongxin Ma 2, Guohua Li 1, Guiping Wang 1,

Xiaorong Liang 1

1.Weifang asthma hospital, Shandong, China K42 2.Weifang people’s hospital, Shandong, China Effect of hyperbaric oxygen to inflammatory cytokine

and pulmonary function in patients with Asthma Objective: To Observe the level of immune cells in the Yongxin Ma 1, Zhaoqiu Hu 2, nong Yu 2 patients with chronic obstructive pulmonary disease and 1. Weifang Peoples Hospital, Shandong, China the influence of hyperbaric oxygen therapy to the immune 2. Weifang Asthma Hospital, Shandong, China function of cells in the stability of patients with chronic obstructive pulmonary disease. Methods: Randomly Objective : To observe the effect of hyperbaric selected patients with stable COPD 167 cases, of which oxygen(HBO) to inflammatory cytokineand pulmonary 86 cases for the treatment group, 81 cases for the control function in patients with asthma. Methods: ninety six group, Tiotropium inhalation aerosol solution, selected 33 patients with asthma were randomly divided into 2 groups: cases of normal healthy people in the control group. HBO therapy group and control group, and another thirty Adoption of improved ABC method in the Observer Group health cases as heath control group, the control group targets immune cells, blood testing, pulmonary function, only accept regular therapy: To inhale Budesonide 200 g after a course of HBO treatment (12 times), to detect the 2 times daily, and HBO group accept HBO therapy and above targets again, and then to give a comparative study regular therapy too. The inflammatory cytokine IL-5, IL-12, analysis. Results: HBO inhalation drug therapy group IL-13 as well as IL-18 were assayed by ELISA after HBO and the control group compared with the normal group exposure. Result: The symptom and FEV1 were targets immune cells have different levels of reduced (P significantly improved after HBO exposure(P <0.005) ,the <0.001), the HBO treatment group after treatment, level of IL-5, IL-13 ,IL-18 were significantly decreased , cell-mediated immunity in patients with various indicators the level of IL-12 was significantly increased. Conclusion: were significantly increased (P <0.01) , and corrected the HBO therapy can ease the bronchial inflammation by CO2 retention and hypoxia (P <0.01), improved lung adjust the inflammatory cytokine excretion, can treatment. HBO 48 hours group got three times HBO significantly improve the patient’s pulmonary function. treatment. Draw blood from the aorta abdominalis after operation at 24h, 48h, 72h respectively, then measure the content of vWF, IL-23 and IL-27 by using enzyme-linked immunosorbent assay (ELISA). Results: (1) The content K43 of vWF in IR 24 hours group increases than that in The effect of HBO treatment on plateletm embrane sham-o group, the difference is significant, P in 24 hours glycoprotein group of IR increases than that in sham-o group, the Ma Linlin difference is significant, P in 24 hours group, 72 hours Beijing Chaoyang Hospital, Beijing, China group of HBO group decrease than those in IR groups at the same time group, the difference is significant, P in 24 Objective: Study the changes of HBO therapy on hours group, 48 hours group, 72 hours group of HBO plateletm embrane glycoprotein, to further reveal the group decrease than those in IR groups at the same time mechanism of HBO therapy and provide a theoretical group, but the difference is not significant, P>0.05. basis with use anti-platelet aggregation in clinical. Conclusions: (1) By using hyperbaric oxygen, the Methods: Through animal tests and clinical studies on the content of vWF decreases at 24h, 48h and 72h, it shows HBO treatment of common indications (Acute carbon that the hyperbaric oxygen has positive effect on relieving monoxide poisoning and delayed encephalopathy, acute ischemic-reperfusion injury. The mechanism is to oxygen poisoning, decompression sickness, ischemic decrease the content of vWF. (2) By using hyperbaric stroke, ischemia-reperfusion injury, SARS after avascular oxygen, the content of IL-23 decreases at 24h and 72h, it necrosis), the positive percentage of CD31, CD61, CD62p, shows that hyperbaric oxygen has positive effect on CD63 and PAC-1 were measured by the flow cytometry inhibiting inflammatory reaction and the mechanism of (FCM). Results: HBO on platelet activation remains hyperbaric oxygen is to alleviate the IL-23 release.(3) In differences. Most studies show that HBO will reduce this experiment, by using hyperbaric oxygen, the content platelet glycoprotein expression, while a small number of of IL-27 has the degressive trend, but has no significant studies have appeared that HBO can increase the meaning. It needs further study. expression of the plateletm embrane glycoprotein to normal. Conclusions: The dual nature of HBO treatment pends further study. HBO treatment with the pressure of time, intermittent, and the treatment of the K45 disease itself? With further study of HBO on platelet Effects of preconditioning with hyperbaric oxygen on glycoprotein, the exact mechanism will become more neural cell apoptosis after spinal cord injury in rats deeply clarified. Peigang Lu, Hua Feng, Yongzhi Xia, Guoqi Gong, Mei Li Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chengdu, China

K44 Objective: To study the effects of preconditioning with The influence of HBO on plasma vWF, serum IL-23 hyperbaric oxygen on neural cell apoptosis and and IL-27 in cerebral ischemic-reperfusion rats expression of Caspase-3 after spinal cord injury in rats at Jiangjiang Lu, Chunjin Gao, Yajuan Li, Baosen Pang, Xiu different time. Methods: fifty-five adult Sprague-Dawley xia Huang adult rats, weighing from 250 to 300g, were randomly Beijing Chao Yang Hospital, Beijing, China divided into 3 groups, hyperbaric oxygen preconditional group(25,n=5) ,normal injury group(25,n=5)and control Objective: By observing the influence of hyperbaric group(5,n=5). In the experimental groups, the rat spinal oxygen treatment on plasma vWF, serum IL-23 and IL-27 cord injury models were established by the way of in cerebral ischemic-reperfusion rats, to explore possible Allen ,s method , and morphological studied by mechanism of hyperbaric oxygen treatment on relieving hematoxylin and eosin staining, Cell apoptosis was ischemic-reperfusion flammatory reaction and to provide detected by TUNEL method and the expression of further experimental evidence for clinical HBO therapeutic Caspase-3 by immunohistochemistry at different time(1 method. Materials and Methods: 64 SD rats were day, 5 day, 7 day, 10day, 14day) were observed. Results: randomly divided into 8 different groups: normal group (N), The apoptostic cells positive for TUNEL and Caspase-3 sham-o group, cerebral ischemic-reperfusion (IR) IR 24 expression were detectable in both two experimental hour’s group, IR 48 hour’s group, IR 72 hour’s group, HBO groups. In the preconditional group, the number of 24 hour’s group, HBO 48 hour’s group, HBO 72 hour’s apoptotic cells and the expression of Caspase-3 were group. Each group contained 8 rats. The cerebral decreased and the neurofunction of the spinal cord ischemic-reperfusion models were established according improved as compared with those in the control group. to Pulsinelli’s methods of blocking four arteries. The There were statistically significant differences between normal group, sham-o group, cerebral the preconditional group and normal injury group ischemic-reperfusion (IR) group was placed in normal especially the control group. Conclusion: Apoptosis of pressure atmosphere without hyperbaric oxygen neural cells is an important morphological change in the treatment. The hyperbaric oxygen group started to accept secondary lesion period after spinal cord injury. HBO hyperbaric oxygen treatment after surgery immediately, preconditioning can reduce the numbers of apoptotic cells and once a day at the same time. HBO 24 hours group got and promote the nerve function recovery in rats after once HBO treatment. HBO 48 hours group got twice HBO spinal cord injury. 1. Department of Pediatrics, the 3rd Xiangya Hospital of Central South University, Hunan, Changsha, China 2. Navy General Hospital, Beijing, China K46 Automatic and non-resistance and non-noise Objectives: To observe the effect of Hyperbaric Oxygen oxygen-supply-and-vent device (HBO) to Human Nerve Stem Cell (hNSCs) Shutian Duan, Xianhua Zhang, Binsheng Ren transplantation on hypoxic Ischemic Brain Damage (HIBD) People’s Hospital, Pingyao County, Shanxi, China on neonatal rats. Methods: Neonatal HIBD rat model was produced according to Rice method. Experimental rat Objective: To reduce resistance and noise and avoid pups were randomly divided into HIBD, cross infection in hyperbaric oxygen therapy. Method: HIBD+transplantation group, HIBD+HBO and Place the detector outside the cabin .Place the air sac and HIBD+transplantation+HBO groups (n=10 in each group silencer in the cabin. Use the detector to detect whether at each time point). On day 3 (d3) after HIBD, hNSCs the air sac is full or not in order to follow the human body were transplanted into the left ventricles by microinjection breath, and supply or vent oxygen . Result: The method. The first HBO treatment was administered within resistance of breathing is largely reduced. The resistance 15min after hNSCs transplantation and once daily for of calm breathing can almost become zero. The noise is consecutively ten days. TUNEL staining was used for greatly reduced. This can avoid cross-infection. apoptotic study at d4, d5, d6, d13 after HIBD. Fluorescent Conclusion: This kind of oxygen-supply-and-vent device immunohistochemical (FIHC) staining and confocal can be used in hyperbaric oxygen therapy. microscopy were used to observe the survival, migration, and differentiation of the implanted cells. Results: (1) On d4, d5, d6 and d13, TUNEL-positive cells could be observed in the injured cortex and hippocampus in all K47 groups. The number of TUNEL-positive cells of Determination of platelet membrane glycoprotein HIBD+transplantation and HIBD+HBO groups were PAC-I and CD63 in patients with delayed significantly less than that of HIBD group (P<0.05). While ecefhalopathy after acute carbon monoxide poisoning the number of TUNEL-positive cells of Xiaomin Hou, Chunjin Gao, Huan Ge, Liming Zhao, Lianh HIBD+transplantation+HBO group was very significantly ua Wu, Yi Zhang, Lin Yang, Zhuo Li, Yu Gao, Man Qi, Ch less than that of HIBD group (P<0.01). (2) FIHC staining engqing Xia showed that on d13, the implanted hNSCs survived and Beijing Chaoyang Hospital, Beijing, China extensively migrated from ventricles to periventricular brain parenchyma, and meanwhile differentiated to Objective: To study platelet membrane glycoprotein neurons and astrocytes. There was no significant PAC-I and CD63 in patients with delayed ecefhalopathy difference about the cell migration style and distribution after acute carbon monoxidepoisoning in order to instruct among HIBD+transplantation, HIBD+HBO and the anti-platelet therapy of DE ACMP. Methods: There HIBD+transplantation+HBO groups. The number of total are DEACMP group and normal control group. The survived cells of HIBD+transplantation+HBO was DEACMP group were draw blood in 7 days after they significantly (P<0.01) more than that of arrived at hospital. The blood of normal control HIBD+transplantation group or HIBD+HBO group. group were drawn in the 8AM after an overnight fast. The Conclusions: (1) After transplantation, hNSCs can positive percentage of PAC- I and CD63 were measured survive, migrate and differentiate into neurons and by the flow cytometry (FCM). Analysis of t-test was astrocytes. (2) To some extent, hNSCs transplantation applied to analyse the expressions of platelet membrane has neuroprotection to HIBD in neonatal rats. (3) HBO glycoprotein PAC-I and CD63. Results: The expressions therapy may enhance the above neuroprotective effect via of platelet membrane glycoprotein PAC- I of the promoting graft cell survival rate, migration and DEACMP group was higher than which in the normal differentiation. control group (P<0.05). The expressions of platelet membrane glycoprotein CD63 of the DEACMP group was much higher than which in the normal control group (P<0.01). Conclusions: According to this study, the K49 expression of PAC-I and CD63 is in a high level with Hyperbaric oxygen therapy for a case of gas delayed ecefhalopathy after acute carbon gangrene in both feet monoxidepoisoning. It indicates that the inhibition of He Xia platelet activation is needed with delayed ecefhalopathy Hyperbaric oxygen department of Chongqing Emergency after acute carbon monoxidepoisoning. Medical Center, Chongqing, China

Objective: Through HBO therapy the gas gangrene. Methods: Air hyperbaric chamber, pressure: 2.5ATA K48 compression time for 40minutes, inhal oxygen for 30 Effect of hyperbaric oxygenation to human nerve minutes, then 10 minutes’ break. After that, another 30 stem cell transplantation on hypoxic ischemic brain minutes’ oxygen inhalation. Further, 50 minutes for damage in vivo decompression with outing of chamber. During the above Mingyan Hei 1, Mili Xiao 1, Zuo Luan 2 procedure, no uncomfortable sense wascomplained. Results: After3hours:T:39C,HR:140bpm,R:30/min,BP:128/58mmH g.After 12 hours, the patient happened tachypnea. T: 39.2 ℃HR: 157bpm, R: 35/min, BP:112/98 mmHg. The patient Objective: The efficacy of strocke dysarthria’ therapy by hyperbaric oxygen. Methods: from 2005 to 2006, 60 cough pink frothy sputum and the both base of lung had patients have divided into two groups.30 patients with the moist rale. Thoracic CT: ⑴ pneumocontusion and hyperbaric oxygen therapy is the observation group, the pneumonedema ⑵pulmonary infection ⑶pleural effusion. other is the control group. 60 patients with the implementation of conventional therapy, the Observation Conclusion: HBO has therapeutic effect on gas group have the hyperbaric oxygen therapy at the basic gangrene pneumonedema. The condition of patient therapy. Results: The effective rate of observer Group is aggravate suddenly may have relation to these factors: 90%, 53.33% control group. The therapeutic effect of the ⑴The patient have no x –ray from admission-time to two groups was significant (P<0.05). Conclusion: going to compartment. It unknown whether decompress hyperbaric oxygen therapy can improve stroke dysarthria conditional will induce reversion under the efficient. pneumonedema. ⑵The systemic inflammatory response syndrome was obvious. ⑶ Chest x-ray of pneumocontusion without clinical syndrome was regarded K52 as contrary contraindecation abroad. The patient Hyperbaric oxygen treatment of persistent vegetative sustained trauma and change of lung x –ray. It can not state 28 cases Experience exclude out pneumocontusion. Guo Guanghan Suqian City of Jiangsu Province Peoples Hospital, Suqian, China

K50 Objective: Study the effects of hyperbaric oxygen on the Effects of hyperbaric oxygen on expression of brain persistent vegetative state patient rehabilitation treatment. derived neurotrophic factor mRNA in hippocampus of Methods: 2001-1/2007-12 Suqian City People's Hospital mice after cerebral ischemia reperfusion of hyperbaric oxygen treatment centre-patient treatment Na Guo, Chunjin Gao, Guozhong Wang, Fujia Liu, Yuepi of 28 cases of pvs patients receiving hyperbaric oxygen ng Lu rehabilitation treatment, using high-pressure air cabin, 0.2 Chaoyang Hospital, Beijing, China mpa pressure, oxygen for 60 min, 10 min middle rest. Results: The rehabilitation of hyperbaric oxygen therapy, Objective: To investigate the effects of hyperbaric basic rehabilitation eight cases, for more then 80 times in oxygen (HBO) on expression of brain derived 6 cases, treatment within 60 to two cases, in the course neurotrophic factor (BDNF) mRNA in hippocampus of within 30d by hyperbaric oxygen therapy in 12 cases, the mice after cerebral ischemia reperfusion and explore the basic rehabilitation of six cases in the 60d via the high therapeutic mechanism of HBO from molecular level. oxygen therapy the basic rehabilitation of two cases. 28 Methods: The mice were randomly divided into a cerebral cases of cases of patients 12-40 years of age, the basic ischemia reperfusion group (IR group), a hyperbaric rehabilitation of six cases ,41-61-year-old with 10 cases, oxygen group (HBO group) and a sham–operation group the basic rehabilitation of two cases, patients over of 61 (Sham group). The cerebral ischemia reperfusion models years of age without basic rehabilitation. Conclusion: were established. The expression of BDNF mRNA in the Hyperbaric oxygen therapy can be recovered in blood hippocampus of mice was measured at 4 h, 12 h, 24 h, 48 pressure, improve microcirculation, help improve h and 72 h respectively, after reperfusion by reverse persistent vegetative state patients awake and function to transcription- polymerase chain reaction (RT–PCR). maintain life. Results: 1) The expression of BDNF mRNA in hippocampus in HBO group was higher than those in the Sham group at 4 h, 12 h, 24 h, 48 h and 72 h (P0.05).3) The expression of BDNF mRNA in HBO group was lower K53 than that in IR group at 4 h (P0.05). Conclusion: The Using Hyperbaric Oxygen as an Adjuvant Method in expression of BDNF mRNA increased in hippocampus of Hypersensivisity Vasculitis mice after cerebral ischemia reperfusion. HBO treatment Huan Ge, Chunjin Gao may effectively relieve the cerebral ischemia reperfusion Department of Hyperbaric Oxygen, Beijing Chaoyang injury through improving the expression of BDNF mRNA Hospital, Capital University of Medical Sciences, Beijing, in hippocampus of mice after cerebral ischemia China reperfusion. Aim: We report a case who was suffered from hypersensivisity vasculitis. Using Hyperbaric Oxygen and corticosteroid to treat hypersensivisity vaasculitis. To the K51 best of our knowledge, this is the first report that The efficacy and nursing research of stroke hyperbaric oxygen (HBO) served as an adjunctive therapy dysarthria’ therapy by hyperbaric oxygen for hypersensitivity vasculitis. Method: A 58-year-old Guo Junmei male patient was injected into his thumb of right hand Second Hospital, Shenzhen, China because of tenosynovitis in local hospital. Half an hour later, he started to have pain and swelling in his distal therapeutic efficacy to patients with Mental Disorder upper extremity of right side. Some medicine were caused by traumatic brain injury. prescribed and for 4 hours without benefit.New lesions developed on the elbow, as well as on the hand. Palpable Purpura was involving anterior aspect of the left upper limb through to elbow. Darkening of the skin also were K55 noted. That night patient was transferred to Beijing Effect of hyperbaric oxygen combined therapy on Chaoyang Hospital affiliated Capital University of Medical persistent vegetative state in 30 patients. Science.Tolazoline 20mg im and Dextran 40 500ml iv Gao Yu, Gao Chunjin, Ge Huan were used to treat spasms of peripheral blood vessels Beijing Chaoyang Hospital, Beijing, China and improve microcirculation by decreasing blood viscosity and impeding erythrocyte aggregation. Objective: To evaluate effectiveness of hyperbaric Dexamethasone meanwhile intravenously 20mg was oxygen combined therapy on persistent vegetative state given. Two hours later the patient’s condition deteriorated and explore the best therapeutic regimen for it. Method: and his lesions developed. HBO therapy (0.2Mpa) was Thirty cases of persistent vegetative state treated with used to alter condition of local hypoxia. After HBO therapy, hyperbaric oxygen combined therapy from 1999 to 2005 the patient’s situation was improved obviously. His were analyzed retrospectively. Including the effects of palpable purpura did not reappeared until 3 hour later. At etiological factors and treatment times and treatment the first 24 hours after admission, the patient was treated juncture on therapy. Result: The excellence rate and with HBO therapy twice. Subsequently, he received HBO improvement rate were significantly higher in cerebral therapy 12 sections (once a day). From the second day, trauma group than those in no cerebral trauma group. And he was orally given 40mg of prednisolone and 200 mg of two groups in which the patients began to receive Aspirin per day for 10 days. Result: The patient’s treatment when became persistent vegetative state for condition was getting better, and palpable purpura and less 61 days recovered better than the other one in which darkening of the skin were also reduced gradually. Fifteen the patients began to receive treatment when became days after treatment with HBO and cortin, the patient’s persistent vegetative state for more 60 days. symptoms and ultrasound finding were normalized. He Conclusions: The effectiveness of hyperbaric oxygen on have a decrease in the dose of prednisolone 30mg/d for the patients with persistent vegetative state caused by 10 days, then 20mg/d until discharge. Conclusion: HBO cerebral trauma and the patients who just begin to receive therapy combine with small doses of corticosteroid is an hyperbaric oxygen treatment when having been ill for less effective and safe therapy for this patient, further studies 61 days better. are needed to evaluate whether HBO can be used as primary therapy for a group of patients suffering from hypersensitivity vasculitis. K56 Efficacy of hyperbaric oxygenation therapy for diabetic foot K54 Bi Zhumei Clinical effects of HBO treatment in Patients with Shandong Provincial Hospital, Jinan, China Mental Disorder Caused by TBI using of BPRS and HDS and ADL Objective: To study the efficacy of hyperbaric Zhaoming Ge 1, Ling Li 1, Hongbin Cai 1, Xudong Zhang 2, oxygenation therapy(HBO) on patients with diabetic foot. Haijun Ren 1 Methods: 42 patients were randomly divided into 1. Second Hospital of Lanzhou University, Lanzhou, conventional therapy group and HBO therapy combined China group, efficacy differences between the two groups was 2. Gansu Provincial Peoples Hospital, Lanzhou, China compared. Results: Cure rate and total effective rate was 68.2% and 86.4% in HBO combined group while it was Background: To evaluate clinical effects of hyperbaric 20.0% and 60.0% in the control group , differences were oxygen (HBO) treatment in patients with Mental Disorder seen between the 2 groups obviously (P<0.05). arising from traumatic brain injury. Methods: One Conclusion: HBO therapy augments the efficacy of hundred and five patients with Mental Disorder following conventional therapy. Traumatic Brain lnjury (TBI) were randomly divided into therapy group(55 cases), which was treated by hyperbaric oxygenation and medications ,and control group (50 cases) which was treated by medications. The scales K57 included the Brief psychiatric rating scale (BPRS) and Effects of comprehensive hyperbaric oxygen Hasegawa Dementia Scale (HDS) and Activity of Daily rehabilitation on function recovery for Living Scale (ADL).All patients were evaluated with BPRS cerebralapoplexy patient and HDS and ADL before and after treatment. Results: NingQin Gao, XiaoPing Chen, Jun Shen, Ying Zhang, Ta Obvious significance in BPRS and HDS and ADL scores oLi Wang, LiHua Huang, HaiPing Ge existed between two groups (after two course of treatment Xuhui Central Hospital, Shanghai, China P <0.05, after the four course and three month P <0.01). Conclusion: The combination of HBO shows good Purpose: Discuss the effects of comprehensive hyperbaric oxygen rehabilitation on function recovery for cerebralapoplexy patient. Method: 50 cerebralapoplexy 2008, 623 papers about the effects of HBO combined patients are divided into two groups, namely, hyperbaric therapy on brain injury were browsed and only 9 trials oxygen and non-hyperbaric oxygen group. The routine (involving 3916 cases) in accordance with the inclusion treatment and rehabilitation is taken for both two groups criteria were reviewed systemically. Among these 9 trials, and the additional hyperbaric oxygen treatment is total effective rates were compared in 8 trials, the scores provided for hyperbaric oxygen group. The functional of Glasgow Coma Scale (GCS) before treatment and one independence measurer (FIM) of patient and simplified month after treatment were compared in 2 trials, and the Fugl-Meyer motion function evaluation method can be mortality rates of the cases within 6 months’ follow-up used for evaluation. Results: The conclusion shows that study after treatment were compared in 2 trials. After the the FIM and simplified Fugl-Meyer scoring difference statistical treatment with the statistics software between both two groups have the statistics significance RevMan4.2, the following statistical outcome was after treatment and the hyperbaric oxygen group is obtained. ⑴ The total effective rate: Clinical effects were superior to non-hyperbaric oxygen group. FIM is reported in 8 trials (involving 3688 cases), and the total 100.92±15.76 after the patient in hyperbaric oxygen group effective rate of HBO combined treatment groups was is treated and FIM is 83.60±28.20 after the patient in markedly improved with statistical significance as non-hyperbaric oxygen group is treated. The comparison compared with that of control groups (95.12% vs. 74.85%; between two groups P±22.61 after the patient in hyperbaric oxygen group is treated and the simplified OR 6.37; 95%CI 5.04,8.06; P<0.01). ⑵ The comparison Fugl-Meyer is 36.64±24.26 after the patient in of the GCS scores: The GCS scores of cases had no non-hyperbaric oxygen group is treated. The comparison statistical significance among groups in 2 trials (involving between two groups P<0.01. The simplified Fugl-Meyer is 430 cases) before treatment and there was no 54.48±22.61 after the patient in hyperbaric oxygen group heterogeneity among groups after Chi square test is treated and the simplified Fugl-Meyer is 36.64±24.26 (P=0.22), but the combining GCS score of cases one after the patient in non-hyperbaric oxygen group is treated. month after treatment in treatment groups was 1.67 more The comparison between two groups P<0.01 than that in control groups one month after treatment and Conclusions: The conclusion is that the hyperbaric the difference had statistical significance (95%CI 1.2,2.14; oxygen treatment can improve the activities of daily living P<0.01),. ⑶ The mortality rates of the cases 6 months and motion function of cerebralapoplexy patients. after treatment: The mortality rates of cases within six

months’ follow-up study after treatment were carried out in

2 trials with 420 cases involved and there was no

heterogeneity among groups after Chi square test K58 Systematic review of the effects of hyperbaric oxygen (P=0.13). It was found that the total mortality rate of cases in 6 months after treatment was 10.76%(24/223) in adjunctive therapy on brain injury treatment groups, which was less than the total mortality Li Chu, Shilian Hu, Oyang Aiqun, Yaowu Ge, Wang Qiang rate (18.78%; 37/197) of cases in control groups with The Second Affiliated Hospital of Anhui Medical University, statistical significance (10.76% vs. 18.78%; OR 0.51; Hefei, China 95%CI 0.29,0.90; P＜0.05),. And NNT(Number Needed to Objective: To evaluate the effects of hyperbaric oxygen Treat) was 12.5 ，which suggests that one patient with （HBO ）combined therapy on the patients with brain injury. brain injury could be avoided from death in 6 months Methods: HBO, as a commonly-used adjuvant among every 12.5 patients if adjunctively treated with therapeutic method to treat the brain injury, is not HBO. Conclusion: After the systemic review of the unanimously accepted by the doctors yet. In this paper, studies of HBO combined therapy on brain injury, it could we searched for the domestically published studies on the be found that the total effective rate significantly improved HBO combined therapy on brain injury and only those and the mortality rate obviously decreased after patients studies in accordance with the following inclusion criteria with brain injury had been adjunctively treated with HBO. were reviewed systemically. ⑴ Randomized controlled So, HBO therapy is a desirable treatment measure for the patients with brain injury, for it proved to be of definite trials (RCTs) and clinical controlled trials (CCTs) about the therapeutic effects and clinical significance. HBO combined therapy on brain injury without confounding factors. ⑵ Trials should contain control groups (medicine/operation combined with HBO) and treatment groups (treated with medicine/operation only). K59 No less than 100 cases were needed in each treatment Clinical study of hyperbaric oxygen treating in group and the case number in control group must meet patients with developing cerebral infarction the statistical requirements. ⑶ The age of each case Du Yuqin Shantou University Medical College First Subsidiary should be between 9 to 75 years old. Statistical Treatment: Hospital, Shantou, China The statistics software RevMan4.2 provided by Central

Cochrane was applied to systematically review the effects Objective: To explore the therapeutic effects and safety of HBO combined therapy on brain injury. Odds Ratio (OR) of Hyperbaric Oxygen treating in patients with developing was applied and 95% Confidence Intervals (95%CI) were cerebral infarction. Methods: 63 patients were randomly calculated. Chi square test was also carried out to assess divided into two groups. The control group (n=30) was the heterogeneity among groups. Results: Up to March treated with Xue Shuan Tong and conventional therapy in different educational background patients (P<0.01). for ACI , and the treatment group (n=33) was treated with Conclusion: Many factors can cause barotraumas of Hyperbaric Oxygen treatment combined with conventional middle ear during HBO treatment to the sudden deafness therapy for ACI , general for two weeks. Results: After the patients. So the operator must pay attention to the above-mentioned therapy , the scores of Nervous complication and take some things to reduce the Functional Deficiency were lower than before therapy and incidence rate of barotraumas of middle ear. the parameters of blood viscosity were decreased in two groups , but were significantly progress and decreased in the treatment group compared with the control group (P<0.01or P<0.05). Conclusion: Hyperbaric K62 Oxygen treatment is a safe and effective method in Effect of different initiation time of hyperbaric patients with developing cerebral infarction. oxygenation therapy on MCAO rats with learning and memory deficits Hao Chen, Zhumei Bi, Ning Cui, Handong Liu Department of Hyperbaric Oxygenation Treatment, K60 Shandong Provincial Hospital, Jinan, Shandong, China Nursing experience in treating h2s poisoning with high pressure oxygen Objective: To investigate the effect of initiation time of Chen Sailian hyperbaric oxygenation (HBO) therapy on spatial learning Department of HBO, the People’s Hospital of Tongling city, and memory deficits of MCAO (middle cerebral artery Anhui, China occlusion) model rats. Method: MCAO rats model was induced. HBO treatment was administered at 6 or 24 To treat H2S poisoning, high pressure oxygen therapy hours after reperfusion respectively in earlier (EHBOT) or should be used as early as possible. High concentration delayed therapy (DHBOT) groups. The spatial learning oxygen inhalation; continuous positive pressure breathing, and memory abilities were evaluated by Morris water artificial sputum, avoiding respiratory tract obstruction are maze test. The infarct volume ratio of each group was the key nurse steps. In the meantime, to change the measured by 2, 3, 5-triphenyltetrazolium chloride (TTC) histanoxia by applying the diutretics, control the staining. Result: In EHBOT and DHBOT group, compared pulmonary edema and encephaledema, use the antibiotic with that in ischemia-control group, the learning and to treat secondary infection are also the effective memory abilities improved and the brain infarct volume measures. The author thinks it is important to use HBO at ratio diminished markedly (all P<0.01). Conclusion: Both early stage and thoughtful nurse in treating H2S earlier and delayed HBO therapy can improve the learning poisoning. and memory dysfunction of rats with ischemia-reperfusion brain injury. Hyperbaric oxygenation therapy shows neural protective effect in ischemia-reperfusion injury.

K61 The reasons and how to prevent barotraumas of middle ear to the sudden deafness patients during hyperbaric oxygen treatment Lijuan Chen, Yan Jiang, Rongzhen Wang, Xiaohong Ding The First Affiliated Hospital, College of Medicine, Zhejiang K63 University, China Hyperbaric oxygen and rehabilitative therapy on severe traumatic brain injury 196 cases Object: To investigate how to prevent barotraumas of Huiping Peng, Xiaoxin Lu, Yongjian Tang, Weihong Fang middle ear during hyperbaric oxygen (HBO) treatment to Fuzhou General Hospital, Fuzhou, China the sudden deafness patients. Methods: There were 1019 patients received HBO treatment in our hospital Objective: To investigate the effective usage of during Jan-04 to Dec-07 and 68 patients among them hyperbaric oxygen and rehabilitative therapy on severe encountered barotraumas of middle ear. The 68 patients traumatic brain injury. Methods: From March 2007 to April were divided into groups according to different reasons of 2008, 196 patients of severe traumatic brain injury were barotraumas of middle ear, first or non-first time treatment treatment by hyperbaric oxygen treatment and and different educational background. And comparison rehabilitative therapy in our department, male 119, female were in progress inter-groups. Results: 6.64% of all 1019 77, average 30.6±8.7years. All patients were severe patients encountered barotraumas of middle ear. Among traumatic brain injury, treated by hyperbaric oxygen them 87% (59 patients) were mild, 11% (7 patients) were treatment (HBOT) and rehabilitative therapy. HBOT middling, 2% (1 patient) was severe. The reasons of pressure was 1.6-0.2Mpa, patients inhale oxygen with barotraumas were eustachian tube obstruct, face mask for 60 minutes, A 5-minute break was taken to inappropriately pressure adjust and faulty operation. breath air inner cabin. HBOT was performed once a day, There was significant difference in composition of these for 10 days as a treatment course. AT the meantime, three reasons. The incidence rate of barotraumas was rehabilitative therapy was performed once or two a day for 5.1% in first time treatment patients, and was 1.6% in every patient, the treatment time was 30-60 minutes, non-first time treatment patients. The difference was including arthrosis passivity movement, nerve muscle significant (P<0.01). There was also significant difference electricity stimulate, physics therapeutics, biofeedback. Result: The number of times of hyperbaric oxygen and Mechanism of ischemic tolerance induced by rehabilitative therapy on patients was 20±4.5; the healing hyperbaric oxygen preconditioning involves rate was 85.2% (167/196); the effective rate was 14.8 up-regulation of hypoxia-inducible factor-1α and %( 29/196).There were no anyone break off treatment for erythropoietin in rats unwell. Conclusion: Hyperbaric oxygen treatment and Gu Guo Jun 1, YunPing Li 1, ZaoYun Peng 1, rehabilitative therapy have significantly effect on severe JiaJun Xu 1, ZhiMin Kang 1, WeiGang Xu 1, HengYi Tao 1, brain trauma patients, they are safety and useful. Robert Ostrowski 2, John H Zhang 2, XueJun Sun 1 Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China

K64 Objective: We studied the effect of hyperbaric oxygen The curative effect of Hyperbaric Oxygenation (HBO) preconditioning on the molecular mechanisms of treatment child brain damage and the influence of neuroprotection in a rat focal cerebral ischemic model. nursing Methods: Seventy two male Sprague–Dawley rats were Jian Bao, Dao-dong Liu pretreated with HBO (100% O 2, 2 atmospheres absolute, The people’s hospital of Lu’an city, Anhui province, Lu’an, 1 hour once every other day for five sessions) or with China room air. In experiment 1, HBO preconditioned rats and matched room air controls were subjected to focal Purpose: Discussion the curative effect of Hyperbaric cerebral ischemia or sham surgery. Post-injury motor Oxygenation treatment child brain damage and the parameters and infarction volumes of HBO preconditioned influence of nursing. Method: In random take out 82 rats were compared with those of controls. In experiment examples as observation group (n=82) from hospitalized 2, HBO preconditioned rats and matched room air child patient (October 2002 to October 2007), and take controls were killed at different time points. Brain levels of the same period 78 examples as compare group. The hypoxia-inducible factor 1a(HIF-1a) and its downstream observation group was given conventional treatment add target gene erythropoietin (EPO) analyzed by Western HBO treatment, the compare group treat only given blotting and RT-PCR as well as HIF-1α DNA-binding and conventional treatment. Observe the difference of curative transcriptional activities were determined in the ipsilateral effect in two groups’ treatment the child brain damage. hemisphere. Results: HBO induced a marked increase in Result : the cure and apparently effective rate of the protein expressions of HIF-1α and EPO and the observation group be higher than compare group. activity of HIF-1a, as well as the expression of EPO Conclusion: The treatment of Hyperbaric Oxygenation mRNA. HBO preconditioning dramatically improved the can raise the effect in child brain damage. neurobehavioral outcome at all time points (3.0±2.1 vs 5.6±1.5 at 4 h, 5.0±1.8 vs 8.8±1.4 at 8 h, 6.4±1.8 vs 9.7±1.3 at 24 h, P<0.01 respectively) and reduced infarction volumes (20.7±4.5% vs 12.5±3.6%, TTC K65 staining) after cerebral ischemia. Conclusion: This Up-regulated HIF-1α is involved in the hypoxic observation indicates that the neuroprotection induced by tolerance induced by hyperbaric oxygen HBO-preconditioning may be mediated by an upregulation preconditioning of HIF-1a and its target gene EPO. Zhaoyun Peng 1, ping Ren 2, Zhimin Kang 1, Jing Du 1, Qinglin Lian1, Yun Liu 1 John H Zhang 1, Xuejun Sun 1 Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical, Shanghai, China

Objective: the present study was to examine the hypoxic K67 tolerance induced by HBO-PC and to explore the role of Hyperbaric oxygen preconditioning attenuates motor hypoxia-inducible factor-1alpha (HIF-1alpha) in a global neuron apoptosis after spinal cord ishchemia in rats: hypoxia model. Methods: Male mice received HBO-PC role of nitric oxide before hypoxia exposure and swimming. Results: Liping Wang 1, Xuejun Sun 2, Wenxian Li 1, Zhimin Kang 2, HBO-PC significantly prolonged the survival time and the Hengyi Tao 2, Weigang Xu 2, John H Zhang 3 tolerance time of swimming under normobaric hypoxia. Department of Diving Medicine, Faculty of Naval Medicine, HBO-PC increased the protein content of HIF-1α and Second Military Medical, Shanghai, China erythropoietin (EPO) in the cerebral cortex and hippocampus and prevented the changes of blood brain Hyperbaric oxygen (HBO) preconditioning confers barrier (BBB) permeability and brain edema caused by neuroprotection against subsequent ischemic insult by hypoxia exposure. Conclusion: The results suggested inhibition to neuronal apoptosis. In this study, we that HBO-PC induced hypoxic tolerance in mice via investigated whether nitric oxide (NO) was involved in up-regulating of HIF-1α and its downstream genes. HBO-induced ischemic tolerance and the underlying mechanisms in a rat model of spinal cord ischemia-reperfusion. HBO preconditioning, which significantly increased NO levels in spinal cord tissue, was K66 produced by consecutive four times of 1-h HBO exposure (2.5ATA, 100%O2) at an interval of 12 h. At 24 h after the last pretreatment, rats underwent spinal cord ischemia for were evaluated before and after treatment. Results: The 9 min. Function recovery was evaluated by 15-point scale, score of NFI after treatment was lower than before and motor neuronal apoptosis was detected by TUNEL treatment in two groups. It was lower in HBO group than staining. Behavioral function was remarkably preserved in control group after treatment. The score of ADL after and motor neuronal apoptosis was notably attenuated by treatment was higher significantly as compared with HBO preconditioning ( P<0.01). Intraperitoneal injection of before treatment in HBO group and control group. After L-nitroarginine-methy-ester (L-NAME, 10 mg/kg), a HBO therapy they were higher than those in control group. nonselective nitric oxide synthase (NOS) inhibitor at 20 Clinical effect wasassociative with the day of beginning min prior to each HBO preconditioning, abolished the and Hunt-Hess grading system. Conclusions: HBO beneficial effects of HBO preconditioning. The activities of therapy and other rehabilitative training were actively caspase-3, -9, -8 and the expressions of mitochondrial used in patients of intracranial aneurysms in the early passway anti-apoptotic mediators in the ischemic spinal stage after operation, could improve neurological function lumbosacral tissue at 6, 12 and 24 h after reperfusion and ADL and quality of life significantly. were evaluated in HBO preconditioned rats with or without L-NAME. NO protected neurons from apoptosis through attenuation of cytochrome c release and caspases cleavage along with upregulation of Bcl-2 and antioxidant K70 enzymes. Intraperitoneal administration of thiol Effect of hyperbaric oxygen therapy in ischemic antioxidant N-acetyl-L-cysteine (NAC, 200 mg/kg) at 15 stroke ：：：150 cases report min before each HBO preconditioning also blocked the Li Li, Jiali Zhang, Yue Yao, Huichun Zheng, Xiaoqin Du neuroprective effects of HBO by decreasing NO HBOT Center of Southwest Hospital, Third Military production. The abovementioned data indicate that NO Medical University, Chongqing, China produced by HBO may contribute to neuroprotection during the preconditioning. Objective: Observe the effect of hyperbaric oxygen

therapy (HBOT) in patients with ischemic brain stroke.

Methods: 150 cases of brain infarction patients, which

performed HBOT, were analyzed retrospectively. Results: K68 The symptoms of 54.0% patients with HBOT were When high pressure oxygen treatment prevents improved significantly, but it is 44.0% in another 150 nursing experience which the middle ear gas crushes cases of brain infarction patients without HBOT during the Gao Xiudong same period. If HBOT could be initiated in 30 days after Victory oil field central hospital high pressure oxygen attacked, the ratio of patients with significant symptoms branch of Dongying, Shandong camp, China improvement is 69.7%, 57.5% in 31-60 days, higher than

in 60-90 days (40.0%) and after 90 days (23.5%). The Objective: Discussion high pressure oxygen branch best outcome was occurred in patients with HBOT course nursing to prevents the middle ear gas to crush the of 21-30 days, 63.6% of which achieved significant morbidity the influence. Methods: We carry on to each improvement, followed by11-20 days (59.7%), less than patient carefully nurse comprehensively, then, the careful 10 days (52.3%) and more than 30 days (31.8%). observation, the record, summarizes, carries on the Conclusions: HBOT can do benefit to patients with brain analysis. Results: Nurses earnestly after us, the patient infarction and it is beneficial to initiate HBOT as early as middle ear gas crushes the disease incidence rate possible, and the course of HBOT should better not less reduces obviously. Conclusion: When high pressure than 20 days. oxygen treatment, carries on carefully nurses comprehensively, but effectively reduces the disease incidence rate which the middle ear gas crushes.

K71

The use of Hyperbaric oxygen in infants external

hydrocephalus comprehensive treatment K69 Wu Xiuyun Effect of hyperbaric oxygen on the rehabilitation of Department of Hyperbaric oxygen Taihe Hospital of neurological function impairment and Ability of Yunyang Medical College, Shiyan, Hubei, China activity of daily living in patients with intracranial aneurysms after operation Objectives: To investigate the role of hyperbaric oxygen Dongjuan Li, Jinlong Liu, Xiaoning Si treatment in infants and young children of the Department of Hyperbaric oxygen, The First Affiliated hydrocephalus and analysis the leading risk factors of Hospital of Sun Yat-Sen University, Guangzhou, China external hydrocephalus. Methods: 100 cases of the

children with hydrocephalus using Hyperbaric oxygen + Objective: To observe the influence of hyperbaric oxygen nerve-brain-training physical rehabilitation treatment, (HBO) combined with physical therapy and acupuncture infants with single oxygen chamber therapy a day, 12 on neurological function impairment (NFI) and ADL in days for a course of treatment, intermittent 20 days , A patient with intracranal aneurysms. Methods: 80 cases total of 2 to 3 course. Treatment of pressure 0 to-4months with intracranial aneurysms were randomly divided into 1.6 ATA; 4-8months 1.7 ATA; 8-12months1.8 ATA; clinical two groups treated with and without HBO therapy besides follow-up from 6 months to 1 year. Results: 100 cases of conventional treatment. The score of NFI and Barthel children with the 2 to 3 course of treatment, clinical one with 2ml of salt water, the experiment lasted for six symptoms disappear, six months of follow-up review of 74 weeks. Then, rabbits in experimental group were cases of head CT subarachnoid fluid decreased to normal, randomized to hyperbaric oxygen (HBO) group(16) and one-year follow-up of 26 cases of non-developmental control group(16). The HBO group receive HBO treatment, disorders and nervous system abnormalities, The total but the control group was placed in the fresh air. At the effective rate was 90%. The risk factors of external end of 2, 4, 6, 8, 10, 12 weeks, the ratio of VEGF, hydrocephalus is the lack of oxygen in perinata, pathological changes of femoral head in light microscope suffocation, encephalitis, trauma, such as the factors that and electron nicroscope, imaging changes were observed. injury the central nervous system. Conclusions: The Results: At the end of 6 th week, a propotion of osteocytes external hydrocephalus children in the comprehensive decreased in volume and the nuclei shrunk. Many treatment on the basis of early supplemented with osteocytes underwent necrosis, dissolved and became high-pressure oxygen treatment, clinical prognosis is fragments. The masculine expression of VEGF was slight. good for children with neural, mental development play a At the end of 12 th week, scattered osteoblast cells could positive role in promoting. be observed around the bone trabecula. Nascent bone cells and collagenous fibri could be found. The ratio of masculine cells of VEGF, which were mainly in the endangium, was increased obviously. At the same time, K72 there were many newborn micrangium. Conclusion: The Nursing during the hyperbaric oxygen treatment HBO can accelerate the vascular developing and after Skin grafting ossification by promoting the exudation of VEGF and Wang Wenjie improve bone repairing. Tai He Hospital affiliated to Yunyang Medical College, Shiyan, Hubei, China

Objective: Explore the relationship between the success K74 rate of skin after transplantation and the nursing during Nursing methods of respiratory tract in severe the hyperbaric oxygen treatment after skin grafting. craniocerebral injury patients after tracheotomy who Methods: 40 patients were studied who took the were treated with high pressure oxygen and reconstruction of skin grafting because of the trauma or combined therapy the organizations after surgeries in our hospital since Ming Huang, Jing Bian 2005. Conventional treatment, nursing and the hyperbaric Department of Neurosurgery, Taihe Hospital of Yunyang oxygen treatment were carried out at the same time. Medical College, Shiyan, China single pure oxygen chamber treatment pressure 0.2 Mpa, or, decompression of the 25min, Regulators 40 min, in a Objective: To discuss nursing method of respiratory tract small cabin air pressure, the pressure of 0.2 Mpa, oxygen after tracheotomy in patients with severe craniocerebral 60 min (30'2 +10'), or, decompression of the 25 min. injury, who were treated with high pressure oxygen and Results: The efficacy is closely related to the time combined therapy, and decrease the infection rate between the transplant and the hyperbaric oxygen respiratory tract and improve the survival rate. Methods: treatment. The time is shorter and the effect is more 198 patients with severe craniocerebral injury were obvious. And the Special nursing during the Hyperbaric treated with tracheotomy and high pressure oxygen and oxygen treatment has played a positive role in promoting combined therapy, all patients were given environmental and prevented the incidence of complications, the nursing, postural nursing, sputum nursing, sputum effective rate was 90%. Conclusions: The Hyperbaric culturing for germs, controlling the correct decannulation oxygen treatment has a significant effect, and should be time and so on. The data was analyzed retrospectively. used earlier during the conventional treatment. Results: All the patients were kept unobstructed of respiratory tract. Infection rate of respiratory tract was 24.9% and survival rate was 89.2%,survival quality was improved obviously and the rate of good was 74.5%. K73 Conclusion: Timely and effective respiratory tract nursing Significance of vascular endothelial growth factor in for patients with severe craniocerebral injury who were steroid-induced vascular necrosis of femoral head performed with tracheotomy can decrease complication of treated with hyperbaric oxygen respiratory tract infection effectively and make for the Cui Cao rehabilitation of patients. Department of Orthopedics, Taihe Hospital of Yunyang Medical College, Shiyan, Hubei, China

Objective: To investigate the stage-changes of vascular K75 endothelial growth factor (VEGF) in the mode of Nursing experience of 26 cases with spinal cord steroid-induced avascular necrosis of femoral head and jnjury treated with hyperbaric oxygen therapy after treated with hyperbaric oxygen, and explore the Dongyun Zhang, Haiju Pi pathogenesis. Methods: 60 adult Japanese rabbits were Department of spinal surgery, Taihe Hospital, Yunyang randomly divided into two groups: experiment group (42) Medical College, Shiyan, Hubei, China and control group (18). As for experiment group, each one was two times per week, and as for control group, each Objective: To investigate the therapy effect of spinal fracture with spinal cord injury treated with spinal cord Objective: To evaluate the clinical effects of hyperbaric decompression and internal fixation and hyperbaric oxygen treatment and rehabilitation training in the United oxygen (HBO) therapy. Methods: From 2001 to 2003, Feng-chi point acupuncture treatment after a stroke 252 cases of spinal fracture with spinal cord injury were pseudobulbar palsy. Methods: 90 patients were randomly treated with spinal cord decompression and AF nail divided into I (ABC). II (ABC) group, were given fixation in our hospital, after operations, 26 cases treated conventional treatment of cerebral vascular disease, and with hyperbaric oxygen (HBO) therapy, and the other 26 another to give I (B), II(B) group hyperbaric oxygen cases treated with routine therapy. The therapy effect was therapy, I (C), II (C) Group and swallowing function of observed according to Frankel standard, and the further rehabilitation training; II(ABC ) Group Feng-chi point of low back pain was also observed, the follow up period’s acupuncture treatment; to their own improved wa tian was one year. Results: The 26 cases treated with surname swallowing function classification standards in hyperbaric oxygen achieved better treatment effect than the treatment of various points in time before and after to the other 26 cases. Conclusion: Spinal fracture with evaluate the patients with swallowing function. Results: In spinal cord injury treated with hyperbaric oxygen therapy addition to I A group, the score of the other five groups in can acquire more better treatment effect than treated only eight points in time have significant differences (P <0.05, with operation. or P <0.001), the score differences between group I and II, therapy group in seven points in time had statistical Significance (P <=0.001); In addition to the three days treatment, I, II group at other times, the group score K76 differences were statistically significant (P <0.05, or P Hyperbaric oxygen treating and caring 48 cases of <0.001). Conclusions: Comparison of clinical, hyperbaric severe brain injury and tracheotomy patients oxygen treatment, more effective than conventional Jing Bian, HouCheng Li therapy group, rehabilitation training group is better than Taihe Hospital, Yunyang Medical College, Shiyan, Hubei, hyperbaric oxygen treatment group, the Joint Feng-chi China point acupuncture treatment group is better than non-acupuncture treatment group. Objective: To explore the nursing experience of tracheotomy and severe brain injury patients during hyperbaric oxygen therapy. Methods: As the 48 cases of severe brain injury and tracheotomy patients who K78 admitted in our hospital in 2005 for the study, during the Hyperbaric oxygen therapy for brain tumors, cerebral conventional surgical treatment and care, recovery vascular tumor after clinical effectiveness analysis supplemented by hyperbaric oxygen treatment, a single Houcheng Li, Ming Huang, Jing Bian, HongPing Ning, Xiu pure oxygen chamber treatment, pressure 0.1 Mpa, Yun Wu, Ming Zhu respectively increased pressure and decompression 25 Shiyan Yunyang Medical College, Taihe, Hubei, China minutes , maintain pressure 40 min, a small, air-pressurized module, the treatment pressure is 0.1 Mpa, Objective: To analyze the clinical effect of Hyperbaric oxygen 60 min, (30 * 2 +10), or, respectively increased oxygen treatment of brain tumors, cerebral vascular tumor. pressure and decompression 25 minutes. Results: on the Methods: Choose 96 patients of brain tumor, cerebral basis of clinical treatment, after vital signs Stability, no vascular tumor who have surgery Since December 2000 further bleeding tendency, as soon as possible to in our hospital's neurosurgical hospital, were randomly hyperbaric oxygen therapy, to promote the lives of divided into two groups. 48 cases of hyperbaric oxygen in comaawakening and improve the life function. Of 48 the conventional drug therapy group at the same time, cases patients, cured 21 cases, markedly effective 13 supplemented by hyperbaric oxygen treatment. Of which cases, improvement 9 cases, 2 cases of invalid, 2 cases 18 cases were single pure oxygen chamber treatment, died, the total efficiency is 91.66 percent. Conclusions: pressure 0.2 Mpa, Regulators 40 min, upward and as soon as possible safe and effective for hyperbaric downward pressure each 25 minutes. 20 patients with oxygen treatment, while the care measures before and small cabin air pressure, pressure 0.2 Mpa, oxygen 60 after hyperbaric oxygen treatment is particularly important, min (30 '× 2 +10'), the middle rest 10 min, upward and and actively deal with complications, can improve the downward pressure each 25minutes. Once a day, 12 prognosis of severe brain injury, has made significant times for treatment. For the longest time, 68 times, the effect. shortest are six times. The control group of 48 cases, conventional drug treatment. Results: HBO were cured 30 cases, markedly effective in 13 cases, improvement in two cases, invalid three cases, 93% efficient. The control K77 group cured 16 cases, nine cases markedly effective, Hyperbaric oxygen treatment and rehabilitation improved in 10 cases, 13 cases invalid. Group 2 cure rate training in the United Feng-chi point of acupuncture and the total efficiency of the differences were statistically Pseudobulbar palsy analysis of the clinical results significant (P <0.05). Conclusion: In the premise of drug HouCheng Li, HongMei Zhang, GuangJian Liu, JunHua treatment, that the surgery patients of brain tumor, brain Wang, ZhongMing Rao, Ming Zhu tumor blood vessels, supported by hyperbaric oxygen Tai He Hospital affiliated to Yunyang Medical College, treatment, clinical treatment more effective than Shiyan, Hubei, China conventional drug group, and the sooner the better network structure upstream activating the function of the therapeutic effect. system, Is conducive to patients regained consciousness. Enhance the liver and kidney function, strengthen the poison of detoxification and excretion.

K79 K81 Nursing of the hyperbaric oxygen treatment to the Study of hyperbaric oxygen therapy on patients reconstructed right hand after operation complicating with disordered brain function and Guiping Hu, Qiong Zhang, Yan Quan incision of trachea Taihe Hospital of Yunyang Medical College, Hubei, China Huijun Hu, Xiaowen Pan Hyperbaric Oxygen Center of PLA, Naval General Objective: To investigate the contribution of hyperbaric Hospital, Beijing, China oxygen treatment to operation achievement ratio of limbs transplantation and the effect on functional rehabilitation. Objective ：：：To study the equipments, therapy opportunity, Methods: A case of dumped hand, after the excision of security and institution of hyperbaric oxygen (HBO) the left upper extremity, was heterotopic transplanted to therapy on patients complicating with disordered brain reconstruct the right hand. Besides the symptomatic treatment and supportive treatment of anticoagulation, function and incision of trachea. Methods ：：：Retrospective dilat blood vessel, anti-inflammatory, analgesia. To carry analysis was done on the patients who complicated with out the hyperbaric oxygen treatment 6 hour after the disordered brain function and incision of trachea from Jan. operation. The pressure is 0.2Mpa(2ATA). The time of 1999 to Jun. 2007. Their data were summarized. Firstly, absorption oxygen is 60 min. Intermedial recess is 10 min. all kinds of equipments through which the patients after Both the process of boosting pressure and tracheotomy could inhale oxygen in HBO chamber were decompressing pressure are 25 min. Deploy seven compared. Their structure, serviceable range, security, treatments in the three days. Then one treatment per day. efficiency of inhaling oxygen and therapeutic In the stage of therapy after operation, the specific nursing efficacy were studied. So the problem on equipments for of keeping the affected extremity warm and raising the the patients was solved. Secondly, all kinds of sputum affected extremity, local proper fixation are very important. aspirators were compared, including their principle, Results: The treatments promote the functional structure, serviceable request and limitations. By using rehabilitation of affected extremity. Half year after of the these sputum aspirators, the problem on aspirating operation, the follow up find that the recovery of the sputum in HBO chamber was solved. So the security of reconstructed right hand is satisfaction. The rotation patients was ensured. Thirdly, we proposed that the function of the antebrachiu: pronate 70 °, supinate 70 ° patients after tracheotomy should be administered HBO the extension, flexion and prehension of fingers are therapy earlier. However, the following factors must be normal. And the muscle force is IV grade. Conclusion: considered, such as the severity of primary diseases, the Prompt hyperbaric oxygen treatment and standard severity of pulmonary infection, the type of HBO chamber, specific nursing after successful operation are the the equipment in HBO chamber, the diathesis and important measures that promote the functional recovery experience of medical personnel in HBO chamber. In of the implanted extremity. addition, informed consent and institution about medical personnel in HBO chamber were very important. Especially for the severe patients, when administered HBO therapy, they must be accompanied by doctors or K80 nurses. Results ：：：After the factors including equipment, Hyperbaric oxygen treatment of toxic encephalopathy medical treatment, nursing, institution and so on had been tetramine case studied and summarized, we solved the problem that the Li Hongyan patients complicating with disordered brain function and The Peoples Hospital HBO Departments of Qitaihe city incision of trachea could be administered HBO therapy Heilongjiang Province, China promptly and safely. Conclusion ：Safety should be the Objective: The implementation of high-pressure oxygen most important premise. Besides, we should make the treatment, toxic encephalopathy and multiple organ failure best of all means to start HBO therapy as early as patients regained consciousness as soon as possible, to possible, through which the patients complicating with language, physical function; to improve multiple organs. disordered brain function and incision of trachea could get Methods: Case Analysis, Hyperbaric oxygen treatment. a better result. Results: After high-pressure oxygen treatment, the patient awareness of recovery, normal intelligence, language physical function returned to normal, liver and kidney function returned to normal. Conclusion: HBO can K82 reduce intracranial pressure, improve cerebral edema. At The foreground of Lidocaine treatment for DCS the same time increase the vertebral - basilar artery blood Li Changchun flow system, thereby increasing the blood supply the brain Navy submarine academy of PLA, Qingdao, China stem, contribute to the restoration of the brain stem With the further cognition to the DCS pathology, some pharmacologic agents raise the people’s interest. Lidocaine is one of the most promising pharmacologic agents because of the obvious treatment effect. K84 Pathologic development of DCS: First, people Observation And Nursing Intervention Of Diabetic’s considered DCS was result from simple physical injury Hypoglycemia With Hyperbaric Oxygen of bubbles; however, following study show the pathology Kong Lei is complicated. It is believed that the body’s host defenses Guangxi Zhuang Autonomous Region People’s Hospital, respond to bubbles as foreign bodies, leading to the Nanning, China activation of enzymes, complement and clotting factors, and the release of vasoactive substances .The activation Objective: To probe into nursing intervention with of the body’s defense system initiates a vicious cycle the diabetic’s hypoglycemia with hyperbaric oxygen. Method: leads to multiple organ failure .To the series of The characteristics of diabetic’s hypoglycemia with inflammation reaction, pharmacologic treatment is very hyperbaric oxygen were analyzed retrospectively and important. Pharmacology of lidocaine: Lidocaine is local nursing countermeasures against it put forward. Result: anesthesia agent,since 1963, it has been used as Hypoglycemia of eight diabetic cases with hyperbaric antiarrhythmic agent for ventricular tachycardia and oxygen got timely treatment, and with consequent fibrillation. It begin to work within 15-30 seconds after remission, continued to take up oxygen till they got intraveneous, and then rapidly distribute to heart, brain, decompressed and discharged from the cabin; thus lung, liver etc. Lidocaine can work on heart directly, but ensuring the HOB treatment. Conclusion: For diabetic’s less effect on vegetable nerve. The present study hypoglycemia with hyperbaric oxygen, it is significant to of lidocaine to treat DCS: It is a tendency to use the give proper health education, dietary guide, medication lidocaine to treat serious DCS, these results from some instruction and warm-up direction. And it is necessary to animal experiments in vivo. In 1984, Evans found understand the patient’s blood pressure, blood sugar, lidocaine can protect nerve system in animal experiment. dieting, medication, metergasis of his liver and kidney in This raises people’s interests to lidocaine as adjuvant order to act on requirements. In the HOB treatment it is treatment for DCS. Subsequence, many scientists also to observe strictly, check hypoglycemia and deal with it proof this view and then study its mechanism in rat and betimes, and following discharging, it is needed to find the rabbit experiments, they found lower-dose lidocaine can cause of hypoglycemia for the purpose of avoiding preserve conduction in isolated nerves and inhibits cation recurring of it. leakage, and inhibit the adhesion of neutrophils to vascular endothelium, but the mechanism is unknown. However, regardless of the mechanism, lidocaine may offer protection by preventing leukocytes from adhering to K85 damaged endothelium. Presently, the Adjuvant Clinical study of assistant treatment with hyperbaric Treatments Committee of the Undersea and Hyperbaric oxygen in reflux esophagitis Medical Society considers the use of lidocaine to be XU Li-min 1, KO Lei 1, ZHOU Qing-nan 2, FENG Dao-rong 1, beneficial in cases of AGE presenting early for treatment. FAN Qiu-ping 1 1. Department of Hyperbaric oxygen, People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, China 2. Department of Gastroenterology, People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, China K83 The near and far-period effect of hyperbaric Objective: To observe the clinical effects of using oxygenation on insomnia hyperbaric oxygen (HBO) to assist in treating 30 patients Kuang Aihua with reflux esophagitis. Methods: 60 patients with reflux Cardiology Department of the Affiliated Hospital to esophagitis were divided averagely into the HBO group Ningxia Medical College, Yinchuan, Ningxia, China and conventional therapy group, both groups were given Omeprazole. Doperidone and L-Glutamine and Sodium Objective: To survey near and far-period healing efficacy Gualenate Granules, while the HBO group received HBO of hyerbaric oxygenation (HPO) in patients with insomnia. in addition to drugs for 8 weeks as a course. Then Method: 84 patients with insomnia without organic compare the improvement of clinical symptom and disease were randomly devided into drug-group and mucous membrane of esophagus, and compare the oxygen-theapy group, and respectively were given drug variation of clinical symptom at 6 month after treatment theapy and HPO theary, Record Pittsburgh Sleep Quality ending, to study the recrudescence rate. Results: The Index (PSQI) of the two groups before and after treatment, effective rate of clinical symptom and mucous membrane and then record and compare/analysis average time of of esophagus were significantly higher inpatients of HBO getting into sleeping and sleeping which is after one group than that in conventional therapy group (p<0.05). month and six months treatment. Result: The effect of Conclusion: Our data suggest that the assistant HPO-theapy group obviously improve than drug-group, treatment with hyperbaric oxygen in reflux esophagitis the effect of after one month treatment and six months may get better clinical results than conventional therapy treatment is similar. Conclusion: HPO can add to and deserve to be widely accepted. effectiveness of the conventional drug-treatment and has a reliable far-period effect. Anti-Aging. In this case the overview of future Medical K86 treatments relies on New Scientific approaches like Effects of hyperbaric oxygen therapy 660 patients Nano-Medicine, Embryonic Stem Cell Transplantation, with craniocerebral injury Chromosomal Gene Insertions and healthy life styles in Wenbo Jin, Min Li, Feng Xu, Ping Wan, Yusheng Ge adjunction with HBOT. Approach: The world is now Department of Hyperbaric Oxygen, Yijishan Hospital, experiencing climatic changes and extreme global Wannan Medical College, Wuhu, China warming not to mention air pollution and shortage of food production and escalating fuel prices. These in effect have Clinical information: 660 patients (465men and 195 presently reduced oxygen concentration at sea level in women) with craniocerebral injury from Jannary 1994 to comparison to past decades. This is mainly due to the August 2007 were studied. high level of carbon particulates in the air and The patients’ ages ranged from 5 to 72 year old. The contamination of drinking water and lesser arable land for course of disease ranged from 6 to 97 days. Causes farming. Consequently oxygen production level is greatly resulting in Injury: Road accident 465, smash and hit reduced. Conclusion: Future medicine inter-links closely accident 142, crash accident 53. with HBOT in Regenerative Anti-Aging Medicine. The control group ,aged from 4 to 70 years old and with the course of disease ranged from 8 to 85 days, was 276 craniocerebral injury patients without undergoing hyperbaric oxygen therapy, No significant differences were observed in terms of Glasgow Coma Scale(GCS) for the age and brain contusion between the two groups. Methods and Results: The K88 pressure was 0.2Mpa (2.0ATA) The high pressure oxygen exposes repeatedly to and pure oxygen was inhaled for 30min × 2 with 10min air organism function correlation influence discussion break by mask. Severe cases with incision of trachea or Jianping Jiang, Yanling Wang low spontaneously breathing undertook first-class oxygen NO.254 Hospital of PLA, Tianjin, China therapy using “humidification sterilization low-resistant device to inhale and discharge oxygen” developed by Objective: For HBO repeated exposure to some of the author. The hyperbaric oxygen therapy (HBO therapy) relevant body to function in a study, for HBO to provide was carried out once a day (special cases twice a the scientific basis for treatment. Methods: High-pressure day), and 10 times as a course. The course of therapy oxygen through clinical observation and animal ranged from 1 to 12 courses, with the average of 2.8 experiments on blood coagulation, the brain erythropoietin courses. The two groups undertook adjunctive therapy of content, liver function, brain trauma neurons ultrastructure dehydration, sufficient hormone, cell activator, correcting of the immune function and the impact analysis. Results: disequilibration of water and electrolyte, enforcing function Conduct clinical hyperbaric oxygen treatment indications training. The curative rates and total effective rates in the correct choice under the premise of the of HBO group were 70.45% and 94.32% , and were implementation of rigorous scientific treatment, HBO significantly higher than the control group 43.48% and treatment is satisfied with the results. However, long-HBO 78.28% (u = 4.61,P ＜ 0.01 ). Conclusions: HBO has therapy on the bodys clotting, liver, and immune and brain functions, such as the impact of the ultrastructure was not become a commonly used therapeutic means for patients obvious at present. Conclusion: How to make more safe with craniocerebral injury, and its curative effect has been and effective treatment of HBO, the volume - the validity confirmed. Our research found that HBO therapy should of our research will need to resolve the issue. got involved in before the peak of the cerebral edema.

Severe cases with incision of trachea or low spontaneously breathing could obtain good therapeutic effect using “humidification sterilization low-resistant K89 device to inhale and discharge oxygen” developed by Effect of related factors on development and the author. Timely and sufficient treatment of HBO, and impact of delayed encephalopathyAfter acute Carbon personalized oxygen inhalation means are the key to monoxide poisoning guarantee the curative effect of patiets with craniocerebral Ji Yunping injury. Tianjin Peoples Hospital, Tianjin, China

K87 CO poisoning delayed encephalopathy occurred in patients with acute poisoning has been sober a few days The role of hbot in regenerative medicine in anti-aging or a few months later. Objective: According by CO Joseph Ting poisoning patients ‘the age, the degree of intoxication, Hi-Tech Hyperbaric Medical Centre, Kuala Lumpur, whether complications, the first high-pressure oxygen Malaysia treatment, treatment programmes, whether cerebral

infarction history, and other relevant factors different on Hyperbaric Oxygen Therapy (HBOT) has been proven to the incidence of delayed encephalopathy different. be of extreme importance in the Human Embryonic Method: I will return to the my hospital Gaoyayangke Stem-cell transplantation and Xeno-transplantation. There treated in the acute Moderate and severe carbon are different aspects of Regenerative Medicine in monoxide poisoning patients in 134 cases, both in the high-pressure oxygen treatment before the quantitative Objectives: To observe the effects of hyperbaric oxygen detection of oxygen hemoglobin > 30%.CT brain Show no therapy (HBOT) on Glasgow coma scale (GCS) in hemorrhage and lesions. In the acute phase are different patients with traumatic brain injury (TBI) and the levels of coma.treatment: in a coma during the entire influences of course and initiating time of HBOT on the process under the pressure of 0.1 Mpa oxygen absorption therapeutic effects. Methods: 105 cases of TBI patients, 2 h. twice daily.After sober changed intermittent oxygen which performed HBOT more than 30 days in HBOT (30 ＇x2 +10 ＇). each Day 1. By age into three groups. A Center of Southwest Hospital, were analyzed retrospectively. The GCS improvements were compared group of 26 cases. 60-year-old. Results: 1. A group of with 29 cases of TBI patients without HBOT during the delayedencephalopathy in four cases (15%). B group, 10 same period. They were also compared between patients cases of delayed encephalopathy (22%), C group, 18 with different severity, initiating times and courses of cases of delayed encephalopathy (32%). two cases of HBOT. Results: The GCS improvement of patients with death. 2. Delayed encephalopathy occurred with the HBOT was 3.97±2.65, especially in severe TBI patients extent of poisoning and hyperbaric oxygen treatment and (5.22±2.49), Both were higher than that without HBOT the first time and whethe complications and whether (2.38±2.16) (P<0.01); The outcome was better in patients cerebral infarction and Brain atrophy and the adequacy of with HBOT course over 50 days (5.03±2.95) than that less the relationship between the show: 134 cases of than 50 days (3.32±2.27)(P<0.01); When HBOT could be recovered patients in 100 cases ,32 cases occurred late initiated in 30 days it was 4.79±2.64, higher than initiated encephalopathy, two cases of death. Delayed recovery after 30 days (3.00±2.34) (P<0.01). Conclusions: HBOT group Compared with that group encephalopathy ,the can improve GCS of patients with TBI. It is beneficial to degree of light poisoning recovery group, with cerebral initiate HBOT as early as possible, and the course of edema and cerebral infarction and cerebral atrophy HBOT should better not less than 30 days. less ,Attending the first time 4h of the 14 cases, high-pressure oxygen treatment time was insufficient.

Conclusion: The greater the age, the incidence of delayed encephalopathy was higher;. level of CO K92 poisoning is more serious, visit the time is nighter, the Hyperbaric oxygen therapy a case of cortical treatment time is not full, the more complications, blindness after coronary angiography particularly that patients with cerebral infarction and HouCheng Li, Yu Liu, HongMei Zhang, cerebral atrophy are the more prone to occur delayed XiuYun Wu, HongPing Ning, Ming Zhu encephalopathy. Tai He Hospital affiliated to Yunyang Medical College,

Shiyan, Hubei, China

A 65-year-old male, at 14:30 on November 27, 2007, K90 because of "coronary atherosclerosis sclerosis", the A non-resistance absorbing oxygen access device femoral artery catheterization, coronary angiography Mingyi Ji, Yingying Liu, Meixiu Feng (CAG) + cerebral angiography. No appeal of discomfort, The second people hospital hyperbaric oxygen section, returned to the sickroom after 1 h, the sense of dizziness, Shenzhen, China following a looking at things fuzzy, flu-gradually

disappeared, binocular vision loss. Immediately to expand Objective: Improve the pipeline which is the second the blood vessels and lowering blood pressure and grade of oxygen pressure reducer in order to reduce the dealing with symptoms, rapid rehydration promote oxygen resistance. Methods: This device accesses contrast agent excretion. Condition not improved, the eye oxygen storaging devices in the original pipeline which fundus examination by urgent consultations "Bilateral absorbs oxygen in the atmospheric with the second grade ischemic optic disc lesions,bilateral retinal artery pressure reducer. Results: Accessing oxygen storaging embolization incomplete." Immediately to hyperbaric devices almost can eliminate the additional resistance oxygen therapy to improve the retina and optic nerve which produced by the second pressure reducer. head ischemic changes, delaying the process of blinding. Conclusion: The accessing devices fully meet the Treatment of pressure 0.2 Mpa (2ATA), oxygen absorption demand that people who is the weak breathing acess oxygen for oxygen therapy via the second grade pressure 60 min (30 〃 × 2 +10 〃), the middle rest 10 min. The reducer, meantime, expand the scope of services and second time after treatment in patients with eyes bright efficacy of the medical equipment. sense of self, in the third treatment, her eyes turning to the people, can clear five fingers.through 20 treatments, binocular vision for the inveatigation Vos1.2, Vod1.0,cured and discharged from hospital. HBO can quickly increase K91 blood oxygen, extend the diffusion distance of the oxygen GCS improvement after hyperbaric oxygen therapy in in the organization , quickly improve and rectify the the traumatic brain injury hypoxic state of eyes fundus organizations , rapid Yue Yao, Fei Li, Mei Li, Xiaoqin Du, Hua Feng restoration of visual function, and saved the eyesight of HBOT Center of Southwest Hospital, Third Military the eyes. Medical University Chongqing, China

K93 The therapy of hyperbaric oxygen combined with 654-2 The Summary of Sudden Deafness Treated By High has distinct effect on treating diabetic foot, no reports Baric Oxygen regarding this new therapy have been published in our Zhang Denghua country or foreign countries. Therefore, further research, Affiliated Hospital of Inner Mongolia Medical College, promotion and application should be made for the Huhehaote, China therapy.

Objective: To understand the present condition of sudden deafness use of high baric oxygen in our country.And all treatment method. Evaluate its result. K95 Method: Collection 200 articles (1999 -2008) about high The change and significance on the contents of baric oxygen treatment sudden deafness with computer In serum interrelated kinases in patients with sever the period of january in. Reading about it, understand the acute CO poisoning during the time of hyperbaric method of high baric oxygen treatment sudden oxygen in treating myocardial damage deafness.and revalue its quantity. Result: Introduced the Hongtu Zhou, Jianguo Yuan, Xuechun Zhang new exhibition in recent 2 years: The sudden deafness is The Affiliated Hospital of Jiangsu University, Nanjing, near concern to the hemal supply. high baric oxygen China treatment can recover the cell function, and can be used as the first choice. The result is better after a week falling Objective: To observe the change of serum interrelated ill. Establish an animal model for suddcn-onsct kinases in patients with severe acute carbon monoxide sensorincural hearing loss(SSNH L). Administration of poisoning (ACOP) during the time of hyperbaric oxygen 3-NP the inner ear through the round window membrane (HBO) treatment. To investigate the mechanism and the can reproduce the clinical manifestation of SSNHL, and effect of HBO in treating myocardial damage. To probe spiral ganglion damage is most prominent pathological the regular pattern of creatine kinase(CK),and its change in this model. Emphasize the result of 3 thesises isoenzyme(CK—MB) during the time of HBO and to that announce recent 2 years, in those thesises, research evaluate the therapeutic effect of HBO. Methods: The objects are the patients each from of author’s hospital. consciousness of 32 patients with severe ACOP was The group of drugs to be for constract and the group of assessed with Glasgow scale 3-7,immediately after the high baric oxygen and drugs to be for exprement. They 1,3,5,10st time HBO therapy. At the same time, these use the standard diagnosis and treatment .And use to patients had a ECG examination. There were another 30 statistics . All the conclusions are positiv. Treating drugs healthy volunteers as normal controls, the contents of choose from 200 thesises. 45kind drugs are divided into 5 serum kinases were measured. Results: With the HBO types: The drugs of blood vessel expansion are 25 kind; treatment time goes up, the patients’ ECG return to The drugs lowering the blood’s glue are 6 kind; Other are normal and the contents of serum CK,CK-MB were 15 kind.Special treatments are 12 kinds. Conclusion: significant decrease(p<0.01). Conclusion: The The data is dependable in 3 thesises; the case's number myocardial damage of patients with severe ACOP can is enough, the method that handles the data is right. The return to normal with HBO treatment. conclusion is dependable therefore.It is importance for group of constract.The research using single drug is more reasonable than reunite of treatment. To choos the best method is one mission in the future. K96 Effects of hyperbaric oxygen on idiopathic sudden sensorineural hearing loss complicated by vertebral artery type of cervical spondylosis K94 Hongtu Zhou, Xiaoping Guo, Wenjie Tan, Jiangguo Yuan, Clinical Research on Treating Diabetic Foot with Xuechun Zhang Hyperbaric Oxygen Combined with 654-2 Affiliated Hospital of Jiangsu University, Nanjing, China Yang Jinhua the first Hospital of Shenyang, Liaoning, China Objective: To investigate the clinical effect of the synthetic treatment of hyperbaric oxygen (HBO) by Objective: To evaluate the curative effect of hyperbaric vertebral artery type of cervical spondylosis (VA-CS) oxygen combined with 654-2 on diabetic foot and discuss resulted in idiopathic sudden sensorineural hearing loss the new effective therapy for treating diabetic foot. (ISSNHL). Methods: 105 ISSNHL patients with VA-CS Methods: Divide349 cases of diabetic foot patients into resulted in vertebrobasilar arterial insufficiency (VAI) treatment group and hyperbaric oxygen group,654-2 weredivided into two groups, the firstgroup includes 59 group and normal treatment group. The treatment group is patients of treatment of HBO and drug is contrast group, treated with hyperbaric oxygen combined with 654-2 and the second group includes 46 patients of treatment of compared with other groups. Results: The curative effect HBO and cervical vertebra traction is HBO colligation and examination index of the group treated by hyperbaric treatment group. Results: The total effective rate of two oxygen combined with 654-2 are significantly superior to groups are 91.5% and 97.8% ( P﹥0.05) . The cure rate of that of other groups (p ﹤0.01) . cure rate and whole two groups are 30.5% and 62.5% (P <0.01). Half year effective rate distinguish to 69.64%, 100%, 34.09%, later, the recurrence rate of follow-up patients of two 80.68%, 30.16%, 75.35%, 24.42%, 40.7%, Conclusion: groups are 33.3% and 6.7% (P<0.05). Conclusions: HBO is one of effective methods to treat ISSNHL. HBO colligation treatment can increase the cure rate and Objective: Figure out the clinical effect of therapy on decrease the recurrence rate. injury of brain by HBO together with acupuncture & healing course. Methods: 48 cases of brain injury to patients were randomly divided into two groups with 22 in group A which was for comparison and 26 in group B K97 which was for observation. The group A received Effects of cervical vertebra traction combined high-pressure oxygen and drug treatment only, the Group hyperbaric oxygen therapy on vertebral artery type of B got acupuncture treatment and healing course besides cervical spondylosis the treatment of the group A. Inducted the Barthel index Hongtu Zhou, Wenjie Tan, Jianguo Yuan, Xuechun Zhan (MBI) to assess the basic ability in normal life as well as g, Tiecheng Shen neurological defects and score Fugl-Meyer motor function Affiliated Hospital of Jiangsu University, Nanjing, China score (MFA) before and after these four weeks. Results: After four weeks of treatment, MBI, neurological Objective: To investigate the clinical effect of hyperbaric impairment and FMA of both two groups have improved, oxygen (HBO) combined cervical vertebra traction with significant differences (P <0.01). Compared group A treatment on vertebral artery type of cervical spondylosis with group B before and after four weeks, MBI, (VA-CS). Methods: 168 patients with VA-CS weredivided neurological deficit and physically FMA had a significant randomlyinto three groups: cervical vertebra traction difference (P <0.05). After three weeks of treatment, total treatment group (group A), 62 cases; HBO treatment efficiency of group B was 92.3 percent, and that of group group (group B), 48 cases; and cervical vertebra traction A was 81.8 percent, respectively, had a significant combined HBO treatment group (group C), 58 cases. The difference (P <0.05), the group B was more effective than patients in each group were treated according to the group A. Conclusion: It is worth further study in designated protocols, and the therapeutic effects were clinical application of therapy on injury of brain by HBO compared among the groups. Results: The total effective together with acupuncture & healing course basing on rates of three groups were 80.6%, 93.8% and 97.8% comprehensive analysis on the significant positive effect respectively. The effective rate of group C was of neurological deficit, motor function and basic ability in significantly better than that of group A normal life after this combined therapy. (PPP<0.01). Conclusions: It is suggested that cervical vertebra traction combinedHBO therapy is an effective method for treatment of VA-CS. The general rehabilitation care can increase the cure rate and decrease the K100 recurrence rate. The clinical observation and the experimental study of the effect of hyperbaric oxygen on peripheral blood stem cell Chenggang Zheng 1, Yajing Zhu 2, Xiaohua Hang 1, K98 Yafei Pang 1, Hongjie Yi 1, Qingle Liu 1 The analysis of clinical effect to hyperbaric oxygen 1. Hyperbaric Oxygen Medical Center, The Faculty of treatment on 23 cases of acute osteofascial Naval Medicine, Second Military Medical University, cornpartment syndrome ．．． Shanghia, China 2. Shanghai Navy 411 Hospital, Shanghai, China Zhou Dun

First Affiliated Hospital to Liaoning Medical University, Objective: To explore the effect of hyperbaric oxygen on Shenyang, China peripheral blood stem cells in Chinese people; to explore

the method in which HBO combined with colony Objective: To analysis the clinical effect of the hyperbaric stimulating factor is used to mobilize peripheral blood oxygen (HBO) treatment of acute osteofascial stem cells. Methods: 1. Employing routine hyperbaric compartment syndmne (OFCS). Methods: 23 cases were oxygen treatment project, we observed the level of the recepted the HBO therapy. Results: The treatment effect peripheral blood CD34 + cells of eight patients who had was positive correlation with starting time ,the effect of received long-term hyperbaric oxygen treatment.We also starting treatment within 24 hour was better then beyond + observed the level of the peripheral blood CD34 cells of 24 hour(P<0.05). Conclusions: Some of cases, which eighteen patients who received hyperbaric oxygen had been treated by HBO therapy in time were avoided treatment because of nervous system trauma, before and the fascial relief incision or amputation with satisfactory after they received hyperbaric oxygen treatment. 2. Thirty results. Balb/c mice were randomly and medially divided into three

groups.In one group(HBO group) mice were exposed to

HBO to mobilize peripheral blood stem cells. In another

group (HGF group) mice were injected with K99 granulocyte-colony stimulating factor (G-CSF) to mobilize Therapy on injury of brain by HBO together with peripheral blood stem cells. In the other group(HBO+HGF acupuncture & healing course group) to mobilize peripheral blood stem cells mice that Ming Zhong, Xueqing Wang, Ping Li were injected with granulocyte-colony stimulating factor The Fifth Affiliated Hospital of Sun Yat-sen University, (G-CSF) at the same time were exposed to HBO.Before Guangzhou and after mobilization the gated cell Scal-1/CD34 dual-positive population was examined by flow cytometer. On the 30 th day after intervention, pure tone threshold Results: 1. After seven times hyperbaric oxygen improvement was 41.36 dB in group I, 43.08 dB in group II, treatment,the absolute count of the peripheral blood 51.70 dB in group III. Furthermore, pure tone threshold CD34 + cells of the patients elevated from (1.87±1.08) improvement among the 3 groups was no statistical ×10 6/L to (4.75±4.67) ×10 6/L,2.54 times than before.After different. Obvious hearing improvement was noted on the fourteen times hyperbaric oxygen treatment,the absolute 10 th day after intervention, no further improvement count of the peripheral blood CD34 + cells of the patients is showed after 20 day intervention. More hearing (2.85±1.94) ×10 6/L,1.52 times than before. After twenty improvement was revealed in the low frequency, while in times hyperbaric oxygen treatment, the absolute count of the high frequency, less hearing improvement was the peripheral blood CD34 + cells of the patients is achieved. Conclusions: Comparison with intravenously (3.26±3.49) ×10 6/L, 1.74 times than before.Compared dexamethasone injection, intratympanic dexamethasone with the absolute count of the peripheral blood CD34 + injection did not provide more hearing improvement on cells of eighteen patients who did not receive hyperbaric patients with profound sudden hearing loss. oxygen treatment,the absolute count of the peripheral blood CD34 + cells of eight patients who had received long-term hyperbaric oxygen treatment did not show statistics difference. 2. In HBO group after mobilization the gated cell Scal-1/CD34 dual-positive population 2.01 K102 times than before.In HGF group after mobilization the One-year efficacy of hyperbaric oxygen for the gated cell Scal-1/CD34 dual-positive population 2.66 patients with acute cerebral infarction times than before. In HBO+HGF group after mobilization Yi Zhang, Chun-jin Gao, Huan Ge the gated cell Scal-1/CD34 dual-positive population 3.76 Beijing Chao-yang hospital, Beijing, China times than before. Conclusions: Hyperbaric oxygen can mobilize peripheral blood stem cells.After seven times, Objective: Observing one year to evaluate the efficacy of fourteen times and twenty times hyperbaric oxygen hyperbaric oxygen(HBO) for the patients with acute treatment, the absolute count of peripheral blood CD34 + cerebral infarction. Methods: 192 patients with acute cells respectively elevates to 2.54 times, 1.52 times and cerebral infarction were divided randomly into hyperbaric 1.74 times than before.After long-term hyperbaric oxygen oxygen group and control group. The neurological exposure, the absolute count of peripheral blood CD34 + impairment was scored before HBO therapy and after 20 cells does not elevate.HBO combined with hematopoietic days, 3 months, 6 months and 12 months. According to growth factors could mobilize peripheral blood stem cells the severity of neurological impairment before therapy, more efficiently. each group was divided into three subgroups. Results: 1.Slight neurological impairment patients: neurological impairment scores of HBO groups were significantly lower than the scores of simultaneous control groups (P<0.05). K101 2. Moderate neurological impairment patients: Preliminary study on intratympanic dexamethasone neurological impairment scores of HBO groups after 20 injection to management of patients with profound days and 12 months were significantly lower than the sudden hearing loss scores of simultaneous control groups (P<0.05). 3. Sever Hui Zhao 1, Tianyu Zhang 1, Jianghua Jing 1, Jining Luo 2 neurological impairment patients: neurological impairment 1. ENT Hospital, Fudan University, Shanghai, China scores of the HBO group after 6 months and 12 months 2. Public Health College, Fudan University were significantly lower than the scores of simultaneous control groups (P<0.05). 4. Both one-year mortality and Objective: to evaluate the effects of intratympanic relapse rate were significantly lower in the HBO group dexamethasone injection on patients with profound than in control group (P<0.05). Conclusion: Early stage sudden hearing loss. Materials and methods: All hyperbaric oxygen therapy can encourage neurological patients in the present study were profound sudden function recovery in one year after acute cerebral hearing loss, initial hearing loss was more than 90 dB, no infarction and lower the one-year mortality and relapse previous interventions was conducted on all patients rate. within 2 weeks onset of sudden hearing loss. Patients were assigned to 3 groups according to patients’ willness, Group I (local and general dexamethasone administration). 2. Group II (Intravenous dexamethasone K103 injection ). 3..Group III (intratympanic dexamethasone Affect of the psychological intervention on parents in injection). In addition, vessel dilation drugs, neurotrophy lower compliance with hyperbaric and hyperbaric oxygen therapy also conducted on all Yan ZHANG, Yun LIU, Gao-Gui HUANG patients. Intratympanic dexamethasone (5mg/ml) injection The Children’s Hospital of Kunming, Kunming, China was performed during 10 days (1 injection /2 days). Pure tone test was conducted on 10 th, 20 th , and 30 th day after Objective: (HBOT) in infants and young children’s clinic. intervention. Results: The factors may impact on the Methods: 67 parents of lower compliance in HBOT had prognosis were matched in all three groups. The threshold psychological intervention. Measured with self-made improvement > 30 dB in group I was 81.82%, in group II compliance questionnaire, to compare the changes of was 83.3%, in group III was 88.64%, statistical study before intervention and after. Results: The attitude of the showed there was no significant different among 3 groups. parents before the intervention: 11 cases of hesitation, 34 cases of not accepting generally, 22 cases of resolutely cases were male, 82 cases were female, average age leaving. The attitude after the intervention: 12 cases of were 36.2±6.7.after consultation of HBO doctors, those willing to accept, 13 cases of generally accepting, 14 who has indication for HBOT, and no counter indication cases of hesitation, 12 cases of resolutely leaving. for HBOT, were given HBOT simultaneously with 2 (X =36.83 ，P<0.01). According to self-made compliance conventional therapy, 126 cases patients who were given the first time HBOT within 2 weeks of admission or questionnaire ，the score is 1.84±0.68 before intervention operation classed as early HBOT group. 108 cases and 2.90±1.44 (t=-5.4454, P<0.01) after intervention. patients who were given the first time HBO treatment after Conclusion: The psychological intervention on parents is 2 weeks of admission or operation classed as late HBOT effective to patients with the lower compliance in HBOT. group. Among which, 92 cases patients who were not To evaluate the effect of the psychological intervention on given HBOT because human factor or economy reason parents in the lower compliance with hyperbaric oxygen served as contrast group. three groups has no statistical treatment. significant difference in sex, age, and GCS score, the follow up time was within 8 weeks of admission or operation. Results: incidence rate of pulmonary infection of early HBOT group was significant lower thanlate HBOT 2 K104 group X =13.78, P ＜ 0.001.late HBOT group was Repetitive hyperbaric oxygen exposures enhance 2 sensitivity to convulsion by upregulation of eNOS and significant lower thancontrast group, X =10.81, P ＜ nNOS 0.005.incidence rate of gastric bleed of early HBOT group 2 Wenwu Liu, Kan Liu, Xuejun Sun was significant lower thanlate HBOT group X =7.32, P＜ Department of Diving Medicine, Faculty of Naval Medicine, 0.01.late HBOT group was lower than contrast group, but Second Military Medical University, Shanghai, China 2 no significant statistical difference, X =3.31, P ＞ Objective: The purpose of this study was to investigate 0.05.incidence rate of renal function lesion of early HBOT 2 the mechanisms in increased sensitivity to convulsions was significant lower than late HBOT group, X =7.39, P＜ and the role of nitric oxide (NO) and its synthases after repetitive HBO exposures. Methods: Mice were randomly 0.01.late HBOT group was lower than contrast group, but 2 assigned into three groups: HBO group; hyperbaric air no significant statistical difference.X =1.15,P ＞ 0.05. (HBA) group and normobaric air (NBA) group. Mice in Conclusions: earlier stage HBOT has significant HBO or HBA group were exposed to hyperbaric oxygen or preventive effects on complications of pulmonary hyperbaric air respectively for 60 min twice daily for 3 infection, gastric bleed and renal function lesion in gravis consecutive days (2.5 atmosphere absolute [ATA]). 24 type craniocerebral injury patients. hours after the last exposure, mice were exposed to HBO (100% O 2, 6 ATA). The latency of convulsions was recorded. In addition, the levels of NO, NADPH diaphorase, mRNA and protein expressions of NOS K106 isoforms in hypothalamus and hippocampus were Hyperbaric oxygen the application value which determined. Results: Latency to seizures was Resucitation to the cardio-pulmonary brian-A report significantly shortened in mice after six HBO of 136 cases pre-exposures. The level of NO in hypothalamus in HBO Zhou Shurong group was increased. The number of NADPH-d positive 1st Affilitaed Hospital of Nanjing Medical Univerisity, cells and the levels of protein and mRNA of eNOS and Nanjing, China nNOS in hypothalamus and hippocampus were increased. Conclusion: After repeated HBO exposures, elevated The hyperbaric oxygen (HBO) applies in the CPCR 136 NO may enhance the sensitivity to convulsions and this cases, in eluding is drowing 23 cases, hanging the neck may lead to seizures during the subsequent oxygen 49 cases, electric shock 3 cases, the narco-operation exposures. Prevention of seizures is needed when HBO is accident 17 cases, Adam-stoke's syndrome 1 case, the used as preconditioning method. drug poisoning 6 cases, asphyxia 6 cases, cover-bedding syndrome 31 cases. Of 66 cases with cardiac arrest, 42 cases were cured, notable effective (2), improvement (1), no effect (21), and effective rate was 68.2%. Of 70 cases K105 with no cardiac arrest, cures 64 cases, improvement (3), Effects of early stage hyperbaric oxygen treatment on no effect (3) effective rate was 95.7%. Elaborated HBO prevention of complications in gravis type treatment mechanism, HBO by its unique for oxygen way craniocerebral injury patients and unique mechanism, has other method of treatment Zhang Ludi the interchange ability in CPCR finally is not the success shanghai sixth peoples hospital which the brain resucitation, regarding this HBO is playing the important treatment role. Reiterated in the treatment Objective: to approach the effects of early stage plan aspect authou before proposed about “the long hyperbaric oxygen treatment (HBOT) on prevention of treatment course HBO therapy may expect caudes some complications in gravis type craniocerebral injury patients. to consider in the past already is patient who hopeless Methods: total 326 cases of gravis type craniocerebral decerebrate condition, so long as is not the genuine brain injury patients whose GCS score ≤8, among which, 244 death, obtains the vitality” experience and idea. The CPCR case of illness suitably in the guardianshipe K108 condition good, rescues in the well appointed large-scale Evaluation of stoke with intellect and memory HBO chamber to carry on the synthesis to treat and cure, curative effect completes accompanies the cabin work, the life symptom Xuming Zhou 1, changes over to the stationary phase, may treat in the Qun Cai 1, Xiuqing Li 1, Liyan Yan 1, Hong Li 1, DanHong So single pure oxygen cabin, must take the HBO in CPCR ng 2 application, raises one batch to be good and under the 1. The Frist Peoples Hospital of Harbin, Ha’erbin, China HBO environment carrying on the rescue work the 2. The second Peoples Hospital of Harbin, Ha’erbin, medical troops. China

Purpose: Detection clinic curative effect of stoke with intellect and memory for HBO. Method: There are 2 K107 groups 132 cases stoke of intellectual deficit and Research on the disinfection of the object surfaces in hypomnesia. Comparison group adopt routine medication which is inside of the hyperbaric oxygen chamber and treatment. Except HBO group routine medication the inner surfaces of its oxygen venting tube treatment under 0.25mpa absorb pure oxygen 60 mins Zhan Hechun (break 10 mins), total 20 days, checking memory quotient Daqing Petrochemical Company employee hospital, (MQ) and hastgama dementia scale (HDS) 2-group China comparison. Between 2 groups comparison show design data of analysis of variance. Result: after 20 days Objective: To explore an individualized omni-bearing treatment, HBO group and comparison group MQ value circulating-target technology on the disinfection of the increased. Diversity is obviously. HBO group got score object surfaces of the hyperbaric oxygen chamber and the more than comparison group. Both of them whether inner surfaces of it’s oxygen delivery the tube to eliminate before or after, diversity is significance of statistics. the possibility of the cross-infection it has been brought by Conclusion: HBO can increase dispersion and tension of the connection ends of the oxygen of the delivery branch oxygen in blood which improve cerebral tissue and urge tube. Methods: To use the innovative process of cerebral tissue recovery. There are protection for white independently delivering oxygen in the multifunctional matter and grey matter. After stoke intellect and memory chamber and th-30 ozone disinfection device, which is is obvious curative effect. indirectly to integrate the process disinfection technology. Under the atmospheric pressure in which is properly close the disinfected chamber’s door, shut off the inlet control valve for oxygen delivery in process flow, open the K109 functional isolation valve of the bypass in process flow, Exploring on the management experience of shut off the control valve in the process flow of oxygen hyperbaric oxygen department delivery, and start the ozone disinfunction device and Shaoxia Zeng, Tao Liu, Liusi Wu, Aiqiong Feng oxygen delivery power device. Results: This integrated Department of hyperbaric oxygen, the first Peoples disinfection technology have reached the requirement and hospital of Zhaoqing, Zhaoqing, China purpose of the target design for both the object surface in the chamber and the inner surface of oxygen venting Objective: To explore the management work of process tube. The research shows that the integrated hyperbaric oxygen department. Methods: Making and process disinfection technology can make unconditioned implementing various management measures of omni-bearing disinfection to the environment inside of the hyperbaric oxygen. Results: The general safety of chamber and to the closed loop of oxygen venting tube, medical hyperbaric oxygen therapy was component it’s safe and reliable, has the efficacy of the individualized through three aspects: equipment operation safety, disinfection, and no secondary contamination source will operating cabin safety and therapy safety; every aspect be produced in the chamber after disinfection. safety need the corresponding scientific management Conclusions: In the process flow of the oxygen directly measure. Conclusion: Only the equipment operation delivered in the chamber, and the ozone disinfection safety, operating cabin safety and therapy safety can device can not be individually used, because the process ensure the successfully developing of safety work on the flow has no oxygen delivery power device to be equipped, department of hyperbaric oxygen. so it’s difficult to have the function of the target disinfection to the inner surface of the oxygen delivery tube. In this case, although the disposable sanitary appliances for oxygen delivery are used, the occurrence of K110 cross-infection still can’t be prevented effectively. Using The rational selection of equipments of breathing in this technology, a new de-bottle-necking means is oxygen on patients after Tracheotomy influencing equipped to the oxygen chamber project; the effectiveness of hyperbaric oxygen treatment unconditioned and zero-risk control of cross-infection in Yu Daoyuan the chamber is realized during the whole process of the Department of Hyperbaric Oxygeon,Jingzhou treatment. Hospital,Tongji Medical College, China

Objective: To explore the rational selection of equipments of breathing in oxygen on patients after Tracheotomy with hyperbaric oxygen (HBO) treatment. Methods: 300 160 patients were randomly divided into two groups patients randomly divided into 2 groups: head cover group according to time of admission: therapy group and control (n=150) and connected tube group (n=150) were given group. The therapy group were treated with HBO and treatment with head cover and connected tube manipulative therapies , the control group were treated equipments, respectively. 209 patients who have with only HBO. Fugl-Meye scores and Brunnstrom grades successfully finished two periods of treatment been were performed before treatment, as well as after 4 and 8 conducted the GCS evaluations. Results: The rates of courses of treatment. Results: The Fugl-Meye scores and success of finishing treatment were 99.3% in head cover Brunnstrom grades of therapy group are obviously higher group, and 59.3% in connected tube group, respectively. than that of the control group. There were significant There was a significant statistical difference in the two differences between two groups ( p<0.05.). Conclusions: groups. The GCS score in connected tube group was The curative effects of HBO combined with manipulative significantly higher than that in head cover group. therapies on limb dysfunction after traumatic head injury Conclusions: For the patients after Tracheotomy with are better. HBO treatment, the curative effect of selecting connected tube is better than that of using head cover. They should choose to use connected tube to absorb oxygen first, and they may choose using head cover only if they can not K113 finish the treatment. Effect of hyperbaric oxygen on the MetHb concentration in patients with acute carbon monoxide poisoning Shifu Yin 1, Chunjin Gao 2, Peisong Wang 3, Huan Ge 2, Lin K111 Yang 2, Yongping Xie 1, Ruquan Jin 1, Effects of early hyperbaric oxygen therapy on Jianmin Liu 1 function recovery in patients with incomplete cervical 1. Gaomi peoples hospital, Shandong, China spinal cord injury 2. Beijing Chaoyang Hospital; Capital University of Yu Youjie Medical Science, Beijing, China Department of Hyperbaric oxygen, People’s Hospital of 3. Department of HBO; Qingdao University Affiliated Guangdong Province, Guangzhou, China Hospital, China

Objective: To investigate the effect of early hyperbaric Objectives: To study the relationship between acute oxygen therapy on function recovery in cervical spinal carbon monoxide poisoning g and methemoglobin and the cord injury patients. Method: 72 patients were randomly influence of hyperbaric oxygen (HBO) on them, and to divided into early hyperbaric oxygen therapy group (E, 36 approach the mechanism of acute carbon monoxide cases) and late hyperbaric oxygen therapy group (L,36 poisoning. At the same time, it gives the new way to the cases).The patients in both groups received the similar clinical treatment of ACOP. Methods: The ACOP group rehabilitation and medicine treatment. The effects were contains 195 patients, divided into the normobaric oxygen assessed by the American Spine Injury Association (ASIA) (NBO) group and the HBO group. The NBO group motor scores, sensory scores and somatosensory evoked contains 97 patients, including 66 mild poisoning patients, potentials (SEP). Result: There was no difference 21 midrange poisoning patient s and 10 severe poisoning between two groups before treatment (P >0 05), and the patients; The HBO group contains 98 patients, including ASIA motor scores improved significantly after treatment 63 mild poisoning patients, 23 midrange poisoning (group E P <0.001, group L P<0.05), but the scores of the patients and 12 severe poisoning patients. The Normal group E was significantly higher than that of the group L control group contains 191 health adults. Detect the (P<0.01). The ASIA sensory scores improved significantly MetHb and COHb concentrations both before treatment after treatment (group E P <0.005, group L P<0.05), but and after treatment in each group and further restriction the scores of the group E was significantly higher than analysis were performed. Results: The MetHb that of the group L (P<0.05). The SEP of the group E concentration is much higher in ACOP group than that in improved significantly than that of the group L (P<0.05). normal control group ( P <0.01). There is dependability Conclusion: Hyperbaric oxygen therapy can facilitate the between COHb concentration and MetHb concentration in function recovery, and it is better to interfere early. ACOP group before treatment ( R =0.418, P <0.01). The MetHb concentration was much lower after HBO treatment than before HBO treatment in ACOP group (P <0.01).To mild and midrange ACOP patients, the MetHb K112 concentration decrease d more significantly in HBO Curative effects of hyperbaric oxygen (HBO) group than that in NBO group ( P <0.05). Conclusions: combined with manipulative therapies on limb The MetHb is probably one of the most important dysfunction after traumatic head injury mechanisms to result in ACOP and makes it serious. HBO Ning Yu, Jian Lu has significant curative effect on decreasing MetHb Department of Hyperbaric Oxygen, The Second People’s concentration. hospital of Nangning, Guangxi, China

Objective: To investigate curative effects of hyperbaric oxygen (HBO)combined with manipulative therapies on K114 limb dysfunction after traumatic head injury. Methods: The nervous system of children infected with the Clinic materials: 1. Case introduction: The cases recovery of hyperbaric oxygen therapy clinical involved burn inpatients in our hospital from Mar 2006 to observation Aug 2007. Among them, there are 36 male and 20 female Yang Zhen with the age range from 1-13 years old and average Tianjin Children Hospital, Tianjin, China 3.6±2.8 years old. The percentage of burn area to total area is between 10-40 and the average percentage is Objective : To observe the high-pressure oxygen 15.1±6.9. They are all healthy before encountering wound treatment on the nervous system in children infected with and have not aspiration injury. 2. Therapeutic methods: the clinical significance. Method : 53 cases of the nervous The 56 patients are randomly divided into two groups: the system of children infected with hyperbaric oxygen treatment group (A group n=28) and control group (B therapy, the use of hyperbaric oxygen therapy at the group n=28). The patients of control group are treated same time to give anti-infection, nerve cell activity only by locally applying Fulikang ointment while those of vasodilative drugs and anti-virus drugs symptomatic treatment group accepted the treatment of local Fulikang treatment. Pressure to 0.06-0.1 Mpa, Regulators 30 ointment plus hyperbaric oxygen (HBO). 3. HBO minutes each for one hour, day, 10 times for treatment, a treatment: Patients of A group accept 100% oxygen total of three treatments, oxygen concentration in more breathing by masks for 80min, once or twice a day. We than 80 percent. Results : high-pressure oxygen stimulated 10d as a therapeutic period and patients were treatment of viral encephalitis after the cure rate is 64.10 arranged therapy according to the healing condition of percent with 25.64 percent efficiency of 10.25 percent wound surface. Normally, one or two therapeutic period efficiency rate of 83.33 percent of cerebral infarction were needed. 4. Assessment Indicator: The information recovered 16.66 percent efficient brain trauma recovery involving the average healing time of wound surface, rate was 75% 25% efficiency of high-pressure oxygen average inpatient day, the size, thick, rigidity and color of treatment of the nervous system The total infection rate scar, the symptom of patients and the number of patients was 97.34 percent. Conclusion: The nervous system who needed operation were collected as the assessment infection in children is common in pediatric clinical disease, indicator. 5. Statistical analysis: All the data were cerebral vascular disease by the virus or the disease analyzed by t test and P<0.05 was considered to be directly caused by brain injury with diffuse inflammation statistically significant. Result: According to the clinic and vascular damage, resulting in brain tissue cycle of observation, in comparison to B group, the scars of A obstacles and edema, increased intracranial pressure So group were rosiness or white, soften, thin, smooth surface that the brain tissue of oxygen and blood supply for the and few pain and itch. And also our findings showed anomaly, a serious disease can be accompanied by a accelerated healing of wound surface and reduced coma. Thus leading to its dominant regional campaign, average inpatient day of group A. Only 2 patients of group feeling the loss of function and language, intellectual A needed flap operation and have achieved the success development have tremendous impact. Through in the first operation, while 9 cases needed operation in B high-pressure oxygen treatment of the nervous system group and among them, the operation of 2 patients were infections in the clinical observation of clinical symptoms failing. The second operation was successful under the improved significantly shorten the duration and reduce the help of 10 times HBO therapy. Discussion: HBO has the rate of invalidity, the resumption of intelligence, thereby following therapeutic effect according to pervious study: 1) enhancing their quality of life, high-pressure oxygen can Increasing the local blood oxygen pressure, relieving or be used as the nervous system in children infected with even rectifying tissue hypoxia, reducing edema and an effective Treatment. improving blood flow; 2) HBO is conducive to abating flap necrosis and enhancing flap survival; 3) In terms of scar formation, the HBO-treated skins represent light-color, K115 soft texture and few pain and itch; 4) Curbing the growth 8 age,male,traffic accident resulted in primary brain stem of some kinds of bacteria. To sum up, HBO is beneficial to infury. Glasgow grade 5. Revivaled after hyperbaric the healing of second-degree burn wound surface in the oxygen treatment. respect of few usage of plasma and albumin, low occurrence of shock and scar formation, reduced tissue necrosism, edema, wound infection and blood poisoning, average inpatient day and the total expense, accelerated wound healing and operative success rate. K116 Clinic Observation of Hyperbaric Oxygen Promoting the Healing of Wound Surface of Second-degree Burn Patients X-L Yang 1, Y-J Deng 2 1. the Department of Hyperbaric Oxygen, Ganzhou K117 Municipal Hospital, Ganzhou, Jiangxi province, China Hyperbaric oxygen treatment pontine brain contusion 2. the Department of Burn, Ganzhou Municipal Hospital, and a case of dissolution of myelin Ganzhou, Jiangxi province, China Yang Wenping Huai’an Second People’s Hospital, Jiangsu, China

Patient: male, 52-year-old, riding on bicycle, the patient accidentally fell down and lead to brain contusion and Obstructive hydrocephalus. The local hospital committed and uninterrupted treatment, so to enhance the effect. after the effects of poor drainage ventricle and Results: Cured 19 cases, obviously effective 45 cases, complicated by lung infection. On October 31, 2006 the effective 73 cases, invalid 13 cases. The total effective patient was transferred to our hospital. Through physical rate is 91.3%. Conclusion: Under the effective nursing, examination, the body temperature was 37.8°, there is obvious effect of cerebrovascular disease patients unconscious, vertical eyes open, body activities with treatment of HBO. disappeared, mouth activities restricted, lung large number of Romanian-moist, having heart rate, limb extension reducing, muscle strength 0, limb tendon hyperre flexia, Bilateral pathological levy negative, liver K119 damage, blood being in lower potassium, sodium, chlorine. The effect of hyperbaric oxygen treatment on the The right lower chest film pneumonia, head CT right coagulation-fibrinolytic system in the patients of frontal lobe brain damage, hydrocephalus, low-density ischemic strok pontine lesions. Through 10-days anti-infection treatment, Lin Yang 1, Wei Wang 2, Chunjin Gao 1, Baosen Pang 1, to dehydration, lung infection was under control, but the Zhuo Li 1 nervous system symptoms had not improved. The MRI 1. Beijing Chao Yang Hospital, Beijing, China inspection pontine basement of T 1 and T 2-signal, the 2. The Third Hospital of Hebei Medical University, patient had 15-years drinking habit, he drunk about 1000 Shijiazhuang, China ML every day. Considering chronic alcoholism liver and brain damage after myelin lead to the dissolution of the Objective To investigate the effects of hyperbaric oxygen CPM, the patient was immediately cured by high-pressure treatment on the Coagulation-Fibrinolytic System in the patients of ischemic stroke. Methods 137 patients with oxygen treatment.0.2 ～0.23MPa O 2 30’×2+10’. During acute ischemic stroke were divided randomly into routine high-pressure oxygen treatment, first three days, twice a therapy group(routine group) ,hyperbaric oxygen day, then once a day, ten days treatment for one course. therapygroup(HBO group). There were 27 healthy After the first time of high-pressure oxygen treatment, the volunteers in control group (control group). We measured patient was conscious and could eyes could open and the plasm concentration of AT-III, TM, uPA and uPAR committed activities as soon as the patient went out of the respectively in the patients with ischemic stroke on the capsule. But 2 days later the patient went unconscious yet 1stand 11thday after admission. Results Compared with till one week later he went back conscious again. After the control group , the plasm concentration of the routine 15-times high-pressure oxygen treatment, the patient group on the 1st day, the 11th day of AT-III decreased,TM could nodded to his family members. After 22-times and uPAR increased;the plasm concentration of the HBO treatment, the patient could communicate with his family group on the 1st day of AT-III decreased , the plasm members in simple speech removing of the trachea, concentration of TM, uPAR of the HBO group on the 1st taking in flowing food; the right upper limb muscle strength day, the 11th day increased (P<0.01) ,TM decreased reached 1 level. Since then the treatment method was (P<0.05) .Conclusion In the patients of acute ischemic changed into a single-capsule pure oxygen chamber. stroke, hyperbaric oxygen treatment can increase the After 4 treatment courses, four limbs muscle tension plasm level of AT-III, and decrease the plasm level of TM increased and muscle strength reached 2 level and liver and uPAR. Thus hyperbaric oxygen treatment can function was normal. After 6 treatment courses, with the effectively improve the function of coagulation-fibrinolytic help of others, the patient could walk by himself, system in the patients with ischemic stroke, and may self-consumption, muscle strength reached 4 level, MRI ameliorate therapeutic effect. basically normal, after six months out of the hospital, the patient fully recovered and could go back to work. From this case we have made the conclusion that high-pressure K120 oxygen treatment can not only have the effective in the The Clinical Analysis of the Hyperbaric Oxygen treatment of brain injury and also have the effective for Therapy in the Patients with Acute Cerebral Infarction recovery of CPM treatment. High-pressure oxygen Lin Yang Chunjin Gao Huan Ge Lianhua Wu Liming Zhao treatment can be used as an effective means and Beijing Chao Yang Hospital recommended for promoting in clinical practice.

Objective To investigate the effect of hyperbaric oxygen

therapy (HBOT) on the patients contracting acute cerebral

infarction(ACI) and to proceed the clinical analysis. K118 Methods Retrospective study was done in 466 Nursing care of cerebrovascular disease patient with hospitalized patients suffered from ACI from Jan. of 2000 hyperbaric oxygen treatment to Feb. of 2005.The patients was divided into HBOT plus Yang Qunfang Wuhan Union Hospital, Wuhan, China drug therapy group (HBO group ) ：303 cases and drug therapy group (control group ) ：163 cases. By using “The Objective: To observe the nursing effect of the European Stroke Scale” (ESS) to score the patients of cerebrovascular disease patients with treatment of HBO group before and after HBOT and to score the hyperbaric oxygen (HBO). Methods: 150 cases receiving patients of control group after hospitalization and before drug therapy were treated with HBO. With the measures discharge. Then HBO group was compared with control of comprehensive care, observing during HBO treatment group. Results For the severe and the moderate ACI, the score difference of HBO group being compared with carbon monoxide poisoning; pregnant; control group were considerably significant (P10) being hypokalemia; hepatitis B virus carriers. compared with control group was significant Glycerol fructose, Cerebrolysin Vial and hyperbaric (P<0.05) .Conclusion Clearly HBOT had a certain effect oxygen therapy were given. treatment plan of HBO: on severe,moderate ACI and vertebra-arteria basilaris pressure of 0.2 Mpa (2.0ATA), oxygen 30 min × 2 + air 10 ACI which were better than drug therapy. The effect on min, time of therapy is 110 min, which boost 20 min, treatment times of HBOT >10 was better than that of deboost 20 min.in the first three days ,2 / day. Later it treatment times ≤10. changed to 1 / days. In the end it took 22 times. on May 1 the patients recovered and were discharged cesarean a healthy boy. 2 Discussion K121 Whether pregnant woman after carbon monoxide Influence of hyperbaric oxygen on the value of poisoning can receive hyperbaric oxygen therapy is still memory quotient and Hastgawa dementia scale in remaining disputes. We believe it should be done . patients with radiation encephalopathy The mechanism may be in: HBO can increase the Yuan Jing oxygen concentration and blood oxygen tension.It can The people’s hospital of zhangjiajie, Hunan, China correct hypoxia, and promote increasing of amniotic fluid, improve foetus development and metabolism. HBO can Objective: To evaluate the effect of hyperbaric oxygen improve uterine blood supply and delay of blood flow so treatment on patient with radiation encephalopathy. To that enhancing the mother-child functional system and study the changes of the value memory quotient (MQ) and placental function. HBO promote gonadal hormones, the Hastgawa dementia scale (HDS) in patients with radiation formation of the placenta villi blood vessels and the encephalopathy pre-and post hyperbaric oxygen function of adrenaline. It conducive to development of the treatment and its value. Method ：：： 60 patients with placentai.The mother’s functions of vital organs can be also improved. It should be noted: hyperbaric oxygen radiation encephalopathy were divided into two groups therapy must be done in time, combined therapy is randomely, hyperbaric oxygen treatment group and important. to observe the condition changes intimately. control group. The values of MQ, HDS in 60 patients were Essential exam must be done before and after HBO measured pre-and post three courses of treament. therapy and Good record must be done. B-monitoring Result ：：：The total effective rate of hyperbaric oxygen should be monitored. premature babies to treatment groups was 86.6% that of control group was high-pressure oxygen therapy can occur eyes of oxygen 60.0%, hyperbaric oxygen treatment group had a good poisoning. Therefore hyperbaric oxygen therapy recovery. After three courses of treatment, the scale of treatment to late trimester of pregnancy should not be too MQ, HDS was increased in both groups, but there was long and should be controlled within 1-2 courses. significant difference between two groups (P<0.05), hyperbaric oxygen treatment group was obvious higher K123 than control group. Conclusion ：：：The treating effect of Comparison of therapeutic effects of HBOT on Severe hyperbaric oxygen combined with drug is much better Brain Injury under 0.15MPa and 0.2MPa than that of drug only and hyperbaric oxygen can further increase the value of MQ, HDS, HBO may be of use in the Chen Jian-hui clinical treatment of RE patients and would benefit from Zhongshan City People's Hospital, Affiliated Zhongshan further study. Hospital of Zhongshan University, Zhongshan, Guangdong Province, China

K122 Hyperbaric oxygen treatment in late pregnancy of Objective: To compare the efficacy of hyperbaric oxygen acute carbon monoxide poisoning at various pressure on severe brain injury ，and to choose PENG Ya, YANG Yulan Guilin, Guangxi NO181 People's Liberation Army Hospital the most reasonable protocol for this desease. Methods: Hyperbaric Oxygen Center The 133 cases of severe brain injury were divided into

March 2008, one case we treated successfully of acute two groups randomly, and they were treated once every carbon monoxide poisoning in late pregnancy, are day by the monoplace chamber. One session a day, ten reported as follows: sessions for one course, all together 30 sesssions for one 1、Case record, F, 23, were unconsciousness after bath a day before patient. But the group A (67 cases) was treated at hospitalized. Physical exam: T:36.7 , HR:90 cpm, RR: 0.15MPa for 60~70 minutes, and the group B (66 cases) 20 cpm, BP: 110/78 mmHg. Pregnant for 7 months. Auxiliary examination: obstetric B-ultrasound showed: was treated at 0.2MPa for 80~90 minutes. We explored fetal heart rate 158 cpm, good movements. ALT (26 u / l), the consciousness and reactions of these patients when GOT (46 u / l), HBsAg(+),potassium (3.27 mmol / l) other they were treated , and reexamine head CT and pass routine examinations were normal. Admission diagnosis: K125 judgment on GCS after each course of treatment is Development of a new inhalation model in hyperbaric finished. Results: There were no significant difference oxygen chamber, between group A and group B in the duration of An Cui, Peisong Wang Linyi City People's Hospital, HBO department unconsciousness, aphasia and Absorption of intracranial Qingdao Medical College Hospital, HBO department hematoma. Conclusions: There were no significant difference in the efficacy of HBOT on severe brain injury The authors triturate a simple breathing patterns in HBO between the protocols at 0.15MPa and 0.2MPa. So champer for adults to inhale oxygen and exhale own carbon dioxide at the same time. See attached map. considering the shorter duration of treatment and the less Principle: Setting that the alveolar ventilation is 350 ml. therapeutic cost we think the lower-pressure protocol is connecting a hard hollow plastic ball , diameter 8.3 cm and volume 300 ml(including the space between mouth better which can treat more patients with the limited and nose mask) ,between the original oxygen masks and resourcs. Y-piece. Patients wear oxygen masks for absorption Less than 200 kpa, at the end of each exhalation there is 300 ml alveolar gas for next inhalation within the hollow ball. K124 The carbon dioxide concentration of alveolar gas is 2.5 Hyperbaric oxygen rescue a case of pulmonary percent in the 200 kpa, the carbon dioxide concentration of inhalation alveolar gas = 300 × 2.5% ÷ 350 = 2%. Due embolism to oxygen consumption, oxygen in the hollow ball is less Li Lingfang than 200 kpa, while hollow ball volume is less than the volume of alveolar ventilation, so when inhale , the Hyperbaric oxygen chamber Liaoning Provincial General ， oxygen through the pipeline influx into the hollow ball Hospital of Fushun Mining Bureau beacuse of difference of partial pressure of oxygen and in order to maintain enough alveolar ventilation, on the end of inhale the oxygen concentration reached 98 percent. A 67-year-old female Patient suddenly occured difficulty in Unless the alveolar ventilation is on big changes, we can breathing, obvious cyanosis, pale face, mydriasis, maintain the relative stability of the concentration of state-dead in the second day after cholecystectomy. oxygen and carbon dioxide concentration. That is, when Multi-parameter monitoring showed that: BP60/40mmHg, the oxygen content increasing, the reinhalation of carbon dioxide can increase blood flow to further improve hypoxia. P139 per minute, R30 per minute, So 258%. Electrocardiogram showed: sinus tachycardia, atrial Comparising the new and old breathing patterns by premature, imperfect right bundle branch block. X chest simulation experiments, there is no obviously difference. film: the thinning of the texture in the inferior lobe of right lung . The patient was diagnosed as acute pulmonary embolism, the vital signs were stable after rescue. But from December 20 to December 23, the K126 patient was in a coma, on ventilator to maintain vital signs Non-resistance oxygen therapy device in mulitplace stable. The first hyperbaric oxygen treatment was given in oxygen chamber the first 10 days after onset. The pressure was 1.8 ATA, Yu Xuelai 15 minutes for compression, oxygen inhalation department of hyperbaric oxygen Nanjing Zijin Hospital (monoplace champer) for 50 minutes, intercalary washing for five minutes, decompression for 20 minutes. After one session of HBO, the patient can open her eyes by calling. The principle of non- resistance device for oxygen therapy After more than 40 times HBO, the patient was used gradient pressure for inhale and exhale oxygen. consciousness, not only can open eyes and speak, but Oxygen pathway: gas source -control board also can answer simple questions. -humidification bottles -flow meter - Simulation of the lung Discussion: - masks (the trigeminal) - patients - oxygen pipeline - HBO can decrease blood viscosity, improve ischemia in outdoors. The patients suitable for non-resistance device embolized regional, and reduce pulmonary avascular for oxygen therapy are including : 1.The patients can necrosis of embolism organizations, and can reduce breath without ventilator. The patients whose pulmonary edema and improve the organs of the state of Respiratory function were relatively poor .2. The high hypoxia, control the occurrence of shock, can reduce paraplegia patients can breath spontaneously whose brain swelling and the brain tissue hypoxia damage, respiratory function were relatively poor.3. Coma patients improve function of the network structure . The HBO can breath spontaneously.4 . patients whose respiratory therapy promote comatose patients to regain center is damaged can breath spontaneously. 5 the consciousness . The HBO therapy plays an important role patients with fractures of the ribs .6 coma patients with in the rescue of patients with pulmonary embolism. tracheotomy and the patients can move by order .7 the patients can use luge type ventilator. The advantage is: easy to operate. Oxygen without resistance. Patient can inhale pure oxygen. Cabin oxygen concentration is not raised. The steps to opreat non-resistance oxygen therapy device: treatment. Control group (B group n=21): the course of 1. Redeploye the pressure of the gas source to 4.0-6.0 Mp. disease was above ten days, the patients’ condition was 2. open the oxygen valve. 3. guide the accompanying staff rather stable and their vital sign was normal. The course to open flowmeter the flow to 400-600 ml, the flow of of their disease was the stage of rehabilitation. Contrast children is depended on the tidal volume. 4. the two groups’ effect. Result: The effective rate of A accompanying staff link oxygen masks and bellows group is significantly higher than that of B group.(p<0.05) (trigeminal tube) to the patient. Conclusion: Earlier and multi-course of hyperbaric The gas path of non-resistance device for oxygen therapy oxygen treatment can shorten awake time, improve is sealed so the gas is pure oxygen. The first class oxygen conscious disturbance, elevate healing rate, lower concentration is 21% +4 × flow / L. disability of disease and raise patient’s quality of life.

K127 K129 HBO cured a case of amnesia hyperbaric oxygen treatment on 3 cases of anesthetic PAN Lin accident coma HBO department of Tianjin Hexi Hospital 300202 Maoxing Yan The department of hyperbaric oxygen, the hospital affiliated to neimenggu medical college The patients is male, 21 years old. memory loss because of losing his love.He had severe insomnia, did not know 3 patients with anesthetic accident coma accepted his family members and classmates, who did not know hyperbaric oxygen treatment combined with drug where he is, did not remember the address, the skull. His treatment from the year of 2005 to 2006, they were cured MRI is normal, so he was diagnosed reactive disorder. and discharged. Neither western medicine nor traditional Chinese 1.clinical dates: The first patient was twenty-seven year medicine took effect. So he came to our hospital and tried female, she accepted appendectomy under general hyperbaric oxygen therapy. two days later, his sleep anesthesia in April, 2005, on the way to ward, she was improved markedly, and his memory recovered.10 days found respiratory arrest and immediately received after the full restoration he returned normal and went back cardiorespiratory resuscitation, ten minutes later, she to school. recovered spontaneously breathing but had been deep Principle of hyperbaric oxygen treatment: high-pressure of coma state, twelve hours later, she was transferred to our oxygen can increase the blood flow of vertebral hospital and immediately accepted hyperbaric oxygen artery ,and increase the relative oxygen partial pressure of treatment, the therapy pressure was 0.25Mpa, oxygen the brainstem reticular activating system. It can activate inhale 30min×2,one time per day, three days later, she neural activity, increase excitatory in EEG and the number was gradually clear, one weeks later, she was completely of fast wave , and extent of frequency.in 0.25 Mpa ,HBO clear, two weeks later, she was cured and discharged, her improve the activities of cytochrome oxidase and Na +-K brain MRI image was normal. Both the second and third +-ATP ,at the same time, it keep the balance of Na + / patients were four year girls, they have been coma Ca2 +, reducing the flow of intracellular, reducing because of congenital heart disease and started to intracellular calcium overload.It normalizes the cellular accepted hyperbaric oxygen treatment on the third day membrane’s function . and the seventh day post-operation respectively, the HBO improves metabolism in sugar, fats, amino acid and therapy pressure was 0.25Mpa, oxygen inhale 30min×2, water, keep electrolyte and acid-base balances, increases one time per day, three weeks and eight weeks the target cells number and functions of insulin receptors. respectively, one person was cured and discharged, the other was clear but remained intellectual disorder and growth retardation. 2. Discussion: Anesthetic accident primarily damages K128 the brain and can make the brain hypoxia and edema. The hyperbaric oxygen treatment in diffuse axonal Hyperbaric oxygen treatment can quickly raise the injury pressure of blood oxygen, improve the brain hypoxia and Yuanbi Yang, Zhuan Wang control the development of the infernal circle of brain Hyperbaric oxygen chamber, Department of neurosurgery, edema. The two patients our discussed accepted The hospital affiliated to Luzhou medical college hyperbaric oxygen treatment rather earlier and got a better prognosis, two-three weeks later, they were cured Objective: Observe the clinical effect of hyperbaric and discharged. The other patient accepted hyperbaric oxygen treatment on diffuse axonal injury, approach the oxygen treatment rather later and got a bad prognosis. suitable therapy opportunity and method. Method: Therefore, when we encounter the anesthetic accident, According to patient's condition and course of disease, we should apply hyperbaric oxygen treatment on the Forty-two patients with diffuse axonal injury were patients as early as possible, which can reduce the randomly divided into two groups: Earlier hyperbaric course of disease and avoid the occurrence of the oxygen treatment group(A group n=21): the course of residual. Concerning the regimen of hyperbaric oxygen disease was from three to seven days, the patients treatment, we all used atmosphere compression chamber separated themselves from life danger under drug control in our text, the pressure is 0.20-0.25Mpa, the time of or post-operation, then they accepted hyperbaric oxygen oxygen inhale is 30min×2, which can get satisfactory the relationship either between therapeutic period and effect and avoid side effects. effect or between therapeutic period and the rehabilitation of intelligence. In this regard, combined with the department of pediatrics, we observed the cases of HIE since 2001 consecutively. The protocol of HBO therapy is K130 0.03 Mpa pressure, 20min for 100% oxygen breathing, Effect of Hyperbaric oxygen therapy on 210 cases of 40min total therapeutic time with twice washing chamber, cerebral trauma once/d and ten times for a period in a single-person, Chunfang Li, Yingyu Pan pure-oxygen chamber. People's Hospital of Haikou City 570208

Objective: To study the clinical effects of hyperbaric K132 oxygen treatment on patients of cerebral trauma. Method: Experience of applying the multi-parameter ECG patients of cerebral trauma were divided into four groups, monitor in hyperbaric oxygen chamber including: 30 cases of acute brain injury, 5 cases of persistent vegetative state, 55 cases of convalescence stage, 120 cases of sequelae stage . Results: after peiyun Li, mingzh Ma treatment 38 patients were cured, 110 cases were People's Hospital of Haikou City markedly effective, 50 cases improved, 12 cases had no effect, and the total efficiency was 94.3%. Conclusion: In the patients of cerebral trauma, hyperbaric oxygen therapy can promote the recovery of consciousness and With the development of medical science, high-tech reduce the sequelae. In the patients of long-term coma, medical equipment has been used more and mor persistent vegetative state and serious nerve damage, extensively in hospitals. Having the advantage of Long course of hyperbaric oxygen therapy can promptness, accuracy and visulization, ECG monitor play significantly improve the effect and prognosis. Additionly, an important role in the diagnosis and treatment of It is most difficult to deal with the patients of severe brain cardiovascular patients and critically ill patients after injury and brain hematoma, as it frequently surgery. It is common to use multi-parameter ECG accompanying vicious increased intracranial pressure, the monitor in some clinical departments, but the application prognosis of patients with cerebral hypoxia was also poor, of this devices in hyperbaric oxygen chamber has been the early hyperbaric oxygen therapy can increase oxygen rarely reported. content in time to prevent the occurrence of malignant From November 2007 to April 2008, we used PM-7000 hypoxia ischemia, providing the possibility of the recovery multi-parameter ECG Monitor to observe 159 cases of of neurological function. Therefore the patients of cerebral critically ill patients in the hyperbaric oxygen chamber of trauma should be given hyperbaric oxygen therapy as YCQ3230-37 we have achieved good results. All the early as possible, to reduce brain injury mortality and ， morbidity, to improve the quality of life of patients. In the equipment are of type PM-7000 made by Shenzhen 210 cases of cerebral trauma, the patients who had Mairui company, the monitoring procedure has been obvious effects after hyperbaric oxygen treatment mostly strictly followed and all the equipment components have were early onset of illness and not serous condition. been checked to make sure they work well, we observed and record the electrocardiogram changes. The application of ECG monitor outside the chamber give critically ill patients more oppptunity for hyperbaric oxygen K131 treatment, expanded the scope of HBOT and improve the Intelligence Deciding the Therapetic Period of safety of patients in the chamber, moreover it is convinent Neonatal Hypoxic-Ischemic Encephalopathy for the medical staff to observe the patients in the chamber which is good for the treatment.

W-Q Fan

K133 HaiKou People’s Hospital 570208 Clinical observation of hyperbaric oxygen treatment

on sixty case of neurasthenia Neonatal hypoxic-ischemic encephalopathy (HIE), the ping yang, guiying he brain damage taking place in the prenatal period, is the Kelamayi Central Hospital, Xinjiang, China normal disease of pediatrics, featured by the high incidence. Destitute of immediate therapy, severe Sixty patients have accepted hyperbaric oxygen treatment neonatal brain can lead to death, irreversible sequelae in our department since the year of 1991, they got and ensuing heavy affliction to family members, increased distinguished effect. We report it as follow: social burden and negative effect to the population quality 1 Clinical dates in our country. The treatment of HIE by hyperbaric oxygen 1.1 General dates: 60 patients between 29 and 66 years (HBO) has been prevailing clinically and achieved better old were included, 20 of them are male, the remains are effect nowadays. However, it is still necessary to probe female, the mean age of them is 47.5 year. The course of disease is between 15 days and 21 years. Case information: the patient was male, 47 years old, 1.2 clinical manifestations: easy to excited, feeling repeating fever for four days, with unconsciousness and weary, sentiment, headache, wakefulness, tension, seizure for two days before admission, accompanying the agitated, the decrease of memory, as well ad impaired symptom of nausea and vomiting, head CT showed concentration. EEG shows that the enhanced excitability density of cerebral longitudinal fissure was high , bleeding of cortex and double side symmetry lower amplitude fast could not be ruled out , then he was transfered to our wave. hospital. Physical examination: temperature: 39.4 ℃, 1.3 Hyperbaric oxygen treatment: The therapy pressure coma, and uneasy, stiff neck, muscular tension of four was 2.0 ～ 2.4ATA, the patients accepted hyperbaric limbs was high, Babinski sign of two sides were oxygen treatment one time per day, oxygen suspicious positive,there were ecchymosis in four limbs. inhale30/×2+5/ per time, one course of treatment was ten Blood white blood cell count showed 19.3 × 10 9 / l, days. neutrophil leukocytes accounted for 93.5 percent, 1.4 The standard of therapeutic effect hemoculture found type 2 Streptococcus suis, confirmed 1.4.1 Curing: The psychiatric symptom and Streptococcus suis disease, meningoencephalitis type. somato-symptom of patients completely disappeared, the He was given dehydration, anti-inflammatory and patients feel well and their occupation and viability can rehydration treatment.He was still unclear after 10 days, recover to anterior sickness. then he received hyperbaric oxygen therapy, once every 1.4.2 Improvement: The psychiatric symptom and day, 80 minutes of oxygen inhalation each time, the somato-symptom of patients generally disappeared, the middle rest of l0 minutes, the absolute pressure was 2.2 patients can be engaged in mental and physical work. ATA, he was in the dim awareness after five days, he had 1.4.3 Invalid: The psychiatric symptom and reaction to question and was agitated; his consciousness somato-symptom of patients remained unchanged. recovered after treatment of 11 days, brain MRI scan had 1.5 Result: After 4-33 times of hyperbaric oxygen no obvious signs of abnormality, hyperbaric oxygen treatment,14 patients’ neurasthenia were cured,44 therapy continued, the patient can walk freely after patients’ condition were improved, 2 patients’ condition treatment of 35 days. weren’t improved, total effective rate was 96.7 percent. Discussion: 2. Discussion This case was acute and had symptom of fever, chills, Hyperbaric oxygen treatment can make the brain tissue general discomfort, headache, nausea, vomiting, obtain sufficient oxygen, improve the metabolic function of unconsciousness, seizure, skin ecchymosis, meningeal neurocyte and lower the excitability of neurocyte, which irritation sign positive, WBC and neutrophil ratio increased, make the physiological activity of cerebral cortex CSF showed Septic change, blood culture found type 2 gradually recover. Hyperbaric oxygen treatment can make Streptococcus suis, diagnosis of pig-borne disease was the blood flow of vertebral artery increase, the pressure of confirmed. on the basis of comprehensive treatment, the oxygen of brain network and brain stem relatively increase, patient was given a daily hyperbaric oxygen treatment, which can effectively improve the attenuation condition of after HBOT of 11 times, the patient was awake in a stable the process of inhibition in cerebral cortex, also can condition. HBO, a non-drug treatment, played an strengthen the function of its regulating and controlling important role in the treatment of this case of human subcortex autonomic nervous system, relieve the infected with pig Streptococcus suis. HBO improves the symptom of autonomic nervous functional disorder. supply of oxygen to the whole brain or the regional lesions, Hyperbaric oxygen treatment can lower the heart rate of help repair damaged cells; hyperbaric oxygen may also the patients who have the symptom of cardiopalmus and break the vicious cycle of inflammatory - hypoxia - pyknocardia, improve the sleep quality of the patients who cerebral edema - increased intracranial pressure , at the have the sleep disorder, improve the senior activity of same time , network activation system has relatively nervous system and raise psychological ability, physical high oxygen pressure, it accelerates the patient’s capacity, intellectual ability, as well as work ability. We recovery from coma. also discover the effect of hyperbaric oxygen treatment on man is better than woman, the short course of disease is better than the long course, the patients who accepted hyperbaric oxygen treatment combined with psychological K135 treatment can obtain better effect. In order to help the Hyperbaric oxygenation and facilitation technique neurasthenia patients early rehabilitate and decrease the combination treatment of a brain diffuse atonal injury complaints, in addition to drug treatment, we can extend Kunru Zhou, Zhihua Yang, Xiuzhen Wang, Jinbo Wang, and apply hyperbaric oxygen treatment on these patients. Shaoxiong Xu Guang Dong Shen Zhen Bu Ji Peoples Hospital, Shenzhen, China

K134 Objective: For the sake of study the therapeutic effect of high pressure oxygen treat pig streptococcicosis,one Hyperbaric oxygenation and facilitation technique cure a case report brain diffuse atonal injury, my hospital carried on the close rongzhen wang xiaohong ding observation into 60 cases of brain diffuse atonal injury the First Affiliated Hospital, College of Medicine,Zhejiang patients from 2003 to 2007. Method: 60 cases of brain University diffuse atonal injury patients are randomly divided into 2 sets. Contrast group (24 cases) was pure to given pressure 0.2 Mpa, wearing oxygen masks inhale pure combined therapy of taking normal regulations medicine: oxygen of 30 minutes, rest for five minutes to inhale the Dehydrating agent depress encephalic pressure; cabin air, and then inhale pure oxygen for 30 adrenocortical hormones activated reticular formation for minutes.Patients got HBOT Once a day of every 10 times awakened cerebrum; correct electrolyte maladjustment; for a course of treatment. A total of 2 to 10 courses, medicine nourish brain cell supply energy; acupuncture follow-up 1 year, then statistic further recurrence rate of and manipulation; HBO group (36 cases) was given cerebral infarction. combined therapy of taking normal regulations medicine Results and hyperbaric oxygenation and facilitation technique, Two patients with cerebral infarction treatment started on the sixth day after the patient hospitalized. comparison in table 1. The course of integrated group and Result: The treatment set cures 25, show effect 7, valid 3, effects of table 2. invalid 1, total efficient 97.5%. The Contrast set cures 8, Table 1 Comparison therapeutic effect of the two groups show effect 4, valid 5, invalid 7, total efficient 70.8%. cured markedlytotal Treatment set efficient is obviously better than the Numb effective effective failed effecti contrast set ;( p<0.05) Reply to diagnose after a year or er number ve rate the telephone track, the treatment set linger effect of of （%） incidence rate is 11.1%. The Contrast set linger effect cases cases % cases% cases % c incidence rate is 58.4%, 2 sets relatively have obvious ases % difference. (p<0.01). Conclusion: Hyperbaric oxygenation and facilitation technique cure a brain diffuse atonal injury earlier, recover quicker, cure effect better. Namely educed disability rate, also reduced death rate. Promote the patient of the function recover, make the integrated group patient be able to return to a society as early as possible. 120 96 80.0 12 10.0 9 7.5 3 2. 5 97.5 Control group 120 48 40.0 36 30.0 21 7.5 15 12.5 87.5 Compared with the control group x2=10.4, P ＜0.01 K136 Table 2 :The course of integrated group with the effects of The Effect of Hyperbaric Oxygen Therapy on cerebral disease infarction course Number cured Markedly effective failed total Pan xiaorong, Fan qiuping of effective effective People's Hospital of Guangxi Zhuang Autonomous cases rate （%） Region 1 ～40 21 15 4 0 100 28 7 13 8 0 100 Our department use hyperbaric oxygen therapy to treat 30days 120 patients with cerebral infarction supplemented with 32 18 13 1 96.87 drug treatment since March 1996 to March 2006, with 31 ～20 4 14 2 90 the same period 120 cases of conventional drug therapy 60days in patients with cerebral infarction contrast, have made 61 ～ significant effect, the report are as follows: 90days 1 information and methods 1.1 general information: 91days ～ 240 cases of cerebral infarction patients, 154 mailes, 86 2.5years females, ages 45 to 77 , an average of 63.5 years old, the Differences between the two groups have a very duration of suffering is from one day to two and a half significant (P <0.01), shows that integrated group relapse years, of which 181 cases of basal ganglia infarction, 22 rate lower than the control group. cases of multiple infarction (Infarction lesions in the three 3 discussion above), lacunar infarction in 37 cases. Randomly divided hyperbaric oxygen, ① blood oxygen content increase ， into two groups: ⑴ HBO therapy and conventional drug blood oxygen pressure increase. Oxygen diffusing treatment group ,120 cases (integrated group); ⑵ capacity and the diffusion distance increase, reducing brain cell degeneration and necrosis due to asphyxia, the conventional drug therapy group of 120 cases (the control effective restoration of ischemia " penumbral regions" group). According to "stroke in patients with clinical neurological impairment score standards" (1995) [2] score. cell function. ② enhanced aerobic metabolism, reduce The two groups by age, sex and treatment of nerve anaerobic glycolysis, reducing brain lactate concentration, impairment and there was no significant difference. and thereby redress acid poisoning, improved the brain 1.2 treatment: environment. ③ contraction of blood vessels, reducing two groups of patients using conventional dehydration drugs decreasing intracranial pressure, blood vessel cerebral edema and reduce intracranial pressure. ④ activation of nerve agents and expansion of vitamin E, promote the formation of collateral circulation. ⑤ and so on. Hyperbaric oxygen treatment: treatment of increase vertebral - basal blood flow and is conducive to Objective: The study was designed to probe the influence the unconscious patients restoration of consciousness. of hperbaric oxygen (HBO) to the serous endothelin in ⑥ lower blood viscosity, promote thrombolisis and patients of cerebral infarction. Methods: Assessing the relationship between the concentration of serous absorption. ⑦ reduce reperfusion injury to the brain. endothelin and therapeutic effect to 64 patients of cerebral Not only hyperbaric oxygen therapy can be used in infarction treated by HBO. Results: the patients within 2w cerebral infarction but also can prevent the cerebral sick have the significantly increased serous endothelin infarction recurrence again, it is an effective, safe and compared with pretreatment (P<0.05), while patients with reliable rehabilitation measures, have important clinical beyond 2w sick have significantly abating serous significance, it is worth promoting. In view of oxygen endothelin （P<0.05 ）. In comparison to the a bit of under high pressure oxygen from the dispersion is still enhancement of control groups, the patients treated by limited, the choice of cases with small lesions will have HBO showed great climbing serous endothelin, especially better effect. those within 2w sick (P<0.05). Conclusion: According to

our study, the beneficial effect of HBO to cerebral

infarction has nothing to do with the decreased serous

endothelin and possible related approaches need to be K137 further explored. Effects of hyperbaric oxygen on sports ability in female middle- long distance athletes before matches

Jian Zhang 1, Lunying Sun 2

1. HBO Department of Shandong Qianfoshan Hospital, K139 Shandong, China A Case Report: the treatment of Hyperbaric Oxygen to 2. PE College of Shandong economic college, Shandong, osteomyelitis China haibin xiang, rong xie

Shanghai Punan hospital 200125 Objective: To explore the effects of hyperbaric oxygen

(HBO) on sports ability in female middle- long distance A female, 8 years old patient went to some hospital in athletes before matches. Methods: Twenty-three female ShangHai for the suddenly occurred weakness of low 1500 meter athletes were randomly divided into limbs and ensuing walking hardly accompanied with hyperbaric oxygen group (HBO group, 11 cases) and disabled urinary and fecal discharge. Physical normal pressure air group (NPA group, 12 cases). Before examination: Reflex of patellar tendon of both sides does matches, eleven athletes (HBO group) were exposed to a not exist. The muscular tension is low and muscle force of hyperbaric environment of 0.2MPa (2.0ATA) to accept a treatment of hyperbaric oxygen. The time of rising the low limbs is level O －Ⅰ with negative Babinski Sign. pressure was 20 min.the athletes inhale pure oxygen for The patient was diagnosed as osteomyelitis preliminarily 60 min, taking a rest for 10 min to breathe air among and hospitalized. Inpatient examination: oligoclonal inhaling oxygen. The time of reducing pressure was 30 antibody of CSF indicated the damaged blood-brain min. The athletes in NPA group were exposed to a barrier and disproportionated increased ratio of IgG. ordinary pressure cabin for 120 min, breathing air. Their Spine MRI showed the abnormal signals(T4 － T11). quiet heart rate (HR), fatigue level, and the results of 1500 Treatment: 200mg methylprednisolone ivgtt for the first meter were determined respectively before and after day, 250mg/d for the second and third day and following experiment. Results: Before experiment, all indexes of prednisone 40mg/d P0 adding the use of γ-Globulin and two groups were not significantly different .After ATP. On the third day, she restored urinary discharge and experiment, the HR of athletes in HBO group were on the ninth day, she had the ability of fecal discharge average lower 3.08±0.83 beats each minute than before with still existing weak low limbs. HBO therapy began with experiment ,and also the feeling of fatigue and the results on the tenth day of hospitalization. After the first period of of 1500m(female) were obviously improved as compaired HBO therapy, patient showed restored urinary and fecal with before experiment. All indexes in HBO group were much more improvement than those in NPA group. discharge completely, enhanced muscle force to level Ⅲ Conclution: Before matches, HBO treatment can －Ⅳ , increased muscle tension, rehabilitated reflex of increase the content of oxygen in blood and tissues , patellar tendon, positive pathological reflex and she could reducing the heart rate, lightening fatigue, adjusting the walk supported by others. The progress after the second function of brain cortex ,and boosting female middle- long therapeutic period involved walking by herself, the distance athletes the motor excitability . asymmetrically induced reflex of patellar tendon and

promoted muscle force to level Ⅳ—Ⅴ. For the ineffective normal treatment, we apply her HBO K138 treatment timely and the significantly increased muscle the Influence of HBO to the Serous Endothelin in force in low limbs suggested the nice synergic function of Patients of Serebral Infarction HBO and normal treatment. The possible effect that HBO H-K Pan treatment have in the case as following: increasing the the First Hospital affiliated to ZhongShan University oxygen pressure and concentration of bone marrow and 510060 enhancing the oxygen extension, which accelerating the rectification of local hypoxia condition; reducing the spinal cord edema; ameliorating the function of blood-spinal cord the liquid crystal display, so it has no high-pressure, and is barrier; abating the tissue adherence after local infection; securer to use in the high-pressure oxygen chamber. prolonging the reproduction period of damaged neural Discussion: We must pay attention to the materials of cells and protecting the irreversibly damaged neural liquid crystal display of the monitor which is used in the tissue which contribute to the rehabilitation of neural cabin and use TFT LCD screen to the greatest possible function; decreasing the occurrence of delayed paralysis extent. Medical electronics, pneumatic equipment which given rise to by the dysfunctional blood flow of the spinal are used in the oxygen chamber could bring about cord. problems of high-voltage, superficial views on the author, related departments should plan as soon as possible, to K140 make detailed laws and regulations of the usage of The discussion of usage of electronic medical electronic medical equipment which is in the hyperbaric equipments in hyperbaric oxygen chambers oxygen chamber, and to ensure the safety of usage. Zhu JianMing Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China K141 When hyperbaric oxygen chamber works, it is a confined Preventive maintenance of equipments and manned pressure vessel, so it is a very important issue hyperbaric oxygen chamber that the proper usage of electronic medical equipments in Zhu JianMing, Yuan YunLai the cabin, which is related to the guarantee of the safety Xin Hua Hospital Affiliated to Shanghai Jiao Tong and effectiveness of the treatment. When the various University School of Medicine, Shanghai, China equipment in the cabin are in an oxygen-rich environment and play the redox reactions, so the board can not have a When hyperbaric oxygen chambers are at work, they are monitor within the EDM, voltage can not exceed 24 V, confined manned pressure containers, so the safety is a which is order to prevent high-pressure environment from very important issue, the paper will be on the way to do a the impact and interference, and accurately monitor study about Preventive Maintenance. Background: It is a patients’ various and real-time physiological information very important issue to ensure the safety of the usage of who are inside the cabin for a long time. Health-care oxygen chambers. In the daily usage of the oxygen workers can observe them convenient inside or outside chamber, routine maintenance work must be done well the cabin, and set and adjust parameters of change at any rather than repair it when it is out of work, which is to time. Multi-parameter monitor is composed of two parts: ensure the safety and effectiveness of treatments. The multiple physiological parameters box which is general at requirements of preventive maintenance: 1. When oxygen the oxygen chamber and is used to measure the patient's chambers are at work, they are in the oxygen-rich ECG, SpO2, NIBP, TEMP, and the physiological environment, so do remember to pay attention to the parameter of the end of breathing carbon dioxide. range of lubricants when using PM. 2. Generally speaking, Through the standard RS232 socket and data a small amount of lubricating oil can be used only when transmission lines, the patient's information can be easily the internal of oxygen chambers in addition to the delivered to the computer workstations outside the revolving door to add flexibility, and other parts for hyperbaric oxygen chamber whose general operating lubricating oil is prohibited. 3. PM asked to reflect "P", is voltage is DC 12 V and can be safely used in the Preventive, so the cycle of doing PM should be classified oxygen-rich environment. The purpose of usage of according to the frequency of usage of hyperbaric oxygen computer workstations is to read the patient information chambers, at least twice a year. The implementation of for analysis, storage, processing and link to HIS systems preventive maintenance: 1. As far as possible to set a in hospitals to share resources. LCD displays are relatively fixed date, do not arbitrarily extend the cycle of generally used in the oxygen chamber, and LCD is PM. 2. In addition to preparing the appropriate cleaning generally divided into two main materials which are called supplies, we must also prepare a strong suction vacuum LCD and TFT respectively. The LCD screen usually cleaner and other equipments. 3. We must prepare needs LED lights background, and also need high-voltage silicone oil according to the characteristics of hyperbaric lights which can light background lights. So the LCD is oxygen chambers. 4. To the devices, instrumentations liquid crystal display of the power supply DC 12 V, but in and equipments inside the oxygen chambers, case work order to supply background lights around 300 ~ 100 V such as cleaning dust should be done. 5. First of all, a test high-pressure, it needs a inverter power supply. It is module should be done on equipments of oxygen precisely this power of the high-voltage inverter, which chambers to observe and monitor the real-time working brings about the hidden dangers to patients who use the conditions for the record, and after the end of PM, we LCD screen of multi-parameter monitors in the hyperbaric should control the situation before and after. 6. Safety oxygen chamber. When the oxygen concentration in the teats, current tests and inspection of the grounding high-pressure oxygen chamber is beyond the standard, impedance should be done to equipments inside the high pressure about 300 ~ 100 V will produce "tinder" chambers. 7. After the end of PM, all work should be on phenomenon, and it is easy to bring about burning record and classified archive for verification in the future. accidents. The TFT (Thin Film Transistor), which is called The discussion of preventive maintenance: It can extend the film field-effect transistor, has made crystal electrodes the life of hyperbaric oxygen chamber through PM, and through using thin-film technology, and control the now the instruments are used within the SMT opening and closing of any one point through using the surface-mount technology to assemble electronic method of scanning. It needn’t use LED lights back to light components, IC-pin is only 1 mm spacing, so if there is dust and plot the event of a humid air, pin indirectly on a one-way valve bumps into three contacts collar extension, larger on-resistance phenomenon have caused the install once again, not bump into the collar extension as breakdown between the pin-damaged. It will be more suitably. (2) Check the interior feeling press diaphragm good rather than harm to do PM regularly, of course, of the lung type oxygen whether it would produce the when doing PM, carefully formulated work must be done, aged chap in the course of time, if aging exchange a new and the equipment within the CMOS circuitry must be diaphragm. (3) The rocking bar valve module like occurs careful noticed and not use vacuum cleaner to suction loses the elasticity, cannot work when the normal work circuit, which will cause the possibility that circuit vacuum scope, must adjust the module operating region or use in cleaner was hit head on the electrostatic Wearing. We exchange the new module. (4) The inspection lung type should take Preventive Maintenance as a main way, and oxygen supply with for the oxygen pipeline attachment take repairing as a supplemented way to extend the point, discovered when has the air leakage phenomenon, service life of hyperbaric oxygen chambers and save must disassemble the attachment point, changes into valuable resources of medical equipments and increase newly the aged O circle. 3. The use of self-flow oxygen in the profits of hospitals. oxygen chamber: In the oxygen cabin uses flows automatically the oxygen general procedure uses the lung type oxygen supply, adjusts the screw rod to move most ambassador the oxygen to supply continuously, supplies K142 the oxygen with the face guard to the patient. This method Analysis of 12 cases of the common fault in author thought improper, not only adjusts the biggest time hyperbaric oxygen chamber oxygen from to supply the oxygen pipe flow to leave also Zhu JianMing, Yuan YunLai can “see the seam from the lung type oxygen supply to Xin Hua Hospital Affiliated to Shanghai Jiao Tong drill” in all directions the overflow, creates in the cabin the University School of Medicine, Shanghai, China oxygen concentration to elevate suddenly. In lung type oxygen supply for an oxygen pipeline connection place When the hyperbaric oxygen chamber works, it is an addition three contacts connection, moreover meets one airtight pressure vessel carries people. The most for the oxygen tube, connects in parallel fashion into a important problem is to make use of security. Based on small oxygen flow idea, follows the current on the gauge my years of maintenance repair experience in medical outlet the direct supplies to have to use flows equipment including hyperbaric oxygen chamber, I will automatically the oxygen patient, instead reduced in the explore the common fault in hyperbaric oxygen chamber oxygen cabin oxygen concentration. 4. the problem in below. The integrity of hyperbaric oxygen chamber is the the use of the rescue of medical equipment in hyperbaric primary guarantee to ensure the use of safety and oxygen chamber. The 220V alternating current supply treatment and to keep the oxygen chamber in the normal cannot enter the oxygen chamber is advantaged in the work state is the prerequisite of hyperbaric oxygen personal safety, but also limited the rescue medical therapy. Below makes a discussion to the common fault in equipment to enter the oxygen chamber. There are many the use of hyperbaric oxygen equipment. Common fault in air operated medical equipment (with rechargeable the use of hyperbaric oxygen chamber subsidiary battery inside) to be possible to supply in the oxygen equipment: 1. At the use of the walkie-talkie of the oxygen chamber to use. That large-scale oxygen chamber needs chamber occasionally leads to “ broadcast ” voice of the frequently to rescue the seriously injured patient in the radio broadcast interference in the middle of the night, oxygen cabin must install the special-purpose fast oxygen would seriously affect the signal communication between connection in the chamber for the oxygen pipeline on. 5. in and outside the chamber. The main reason is 220V the The common problem on the equipment supplies the city electricity 50Hz power frequency disturbance string oxygen in the hyperbaric oxygen chamber. Enters the amplifier, returned to original state “the broadcast sound”. cabin after the oxygen pipeline to divide into the left and Solution: (1) Improve the grounding of the walkie-talkies right two group supplies lung type oxygen supply, such to make the grounding more reliable. (2) Place a capacitor procedure can produce inevitably for the oxygen pipeline capacity from 0.01 f to 0.01 f on the secondarily power “the blind end”, causes “the blind end” on patient to attract transformer of the walkie-talkie in order to reduce the the oxygen to feel the hard sledding. Installs one in each disturbance phenomenon by the distributed capacitor. lung type oxygen supply for the oxygen pipeline (3) Use the transformers with center shielding layer attachment point “the cushion pot” to solve, “the cushion between first and secondary to replace the original one. pot” must have the volume which the enough big like 2 (4) Change the moving coil microphone and speaker litres control. 6. The problem in the installment of the lung which used in chamber into the piezoelectric ceramic chip. type oxygen supply in oxygen chamber. In the oxygen 2. The lung type oxygen used in oxygen chamber is cabin the lung type oxygen supply installment direction specially designed for the driver attracts the oxygen common is all kinds, there are the top and bottom equipment, needs about 300Pa negative pressure to turn installment and the left and right one. The correct on the rocking bar valve to attract the oxygen, when no installment direction of lung type oxygen supply should be oxygen absorption in failure, refer to the following steps to according to supplies under the oxygen tube vertical inspect: (1) Inspects whether the rubber cone-way valve dynasty, adjusts the screw rod to be vertical in on installs. piece in the three-way pipe has the aging and anti-roll or This way is the most reasonable one, the patient attracts shifting bumps into attracts the oxygen tube collar when the oxygen not only depends on the negative extension. (Row of oxygen breakdown reverse inspection pressure the function, moreover has the action of gravity row of oxygen tube.) If found the rubber one -way valve help to make an effort slightly. 7. The daily adjustment has the aged phenomenon, exchange a new one. If the method of the lung type oxygen supply. From attracts on the oxygen tube the plastic three-way pipe to draw out therefore the graphite, the micro granulated activated places nearby the ear on, adjusts the screw rod carbon does not suit in the oxygen cabin air feed air dextrorotation, when just heard “si si” leaked the oxygen strainer to use. sound, the screw rod maneuver half-turn, the lung type oxygen supply is adjusted by now in “the critical point”, attracted the oxygen to be at the optimum condition to make an effort slightly. 8. The problem in the supply of K143 oxygen source in the oxygen bottle. The problem Curative effect of the treatment with HBO for produced by the valve gasket which between the bus and ulcerative colitis the oxygen cylinder is that in the use of oxygen bottles Zhu Hourong (pressure is 15MPa/150KPa) as a source of oxygen NanJing JiangNing Hospital, Nanjing, China supply, because of the use of nylon or Teflon used as the gasket material for valve interior, some articles reported Objective: To evaluate the curative effect of the frequently that a valve gasket in the combustion. The combined treatment with Sulfasalazine and Hyperbaric ignition point temperature of Teflon is 300 ℃, not easy to oxygenation (HBO) for ulcerative colitis. Methods: 38 cases with light or moderate ulcerative colitis at active burn, so it is safety to use the gasket which is made by stage were randomly divided into 2 groups, treatment material of the pure Teflon, but uses the gasket which the group(n=19) receiving sulfasalazine and HBO, and control material does on is easily to be a burning accident. group(n=19), receiving sulfasalazine only, the curative Completely change the Teflon gasket in the oxygen effect, the reoccurrence in 6 months,the change of main supply pipeline into the red copper material so that once and for all solves the burning hidden danger which symptoms before and after treatment were evaluated 。 possibly exists. 9. Console oxygen supply oxygen Results: The total effective rate of treatment group chamber pressure gauges, indicators can not return to the (89.47%) was significantly higher than that of control issue. Installs an evacuation valve between the secondary group(68.42%) (P<0.05), there existed an obvious reducer and the control bench for the oxygen valve can difference in symptom relief, such as abdominal pain, solve the problem. After the daily payload separation diarrhea, mucus bloody stool and tenesmus, between the ended, close the oxygen valve then open the evacuate 2 group after treatment (P<0.05). Conclusions : the valve, drain away completely the pipeline in remaining combined treatment with Sulfasalazine and HBO is of oxygen causes the indicator to return the zero. It can better curative effect for ulcerative colitis and it can reduce extend the life of the oxygen pressure gauge that close the reoccurrence. the oxygen valve and then immediately evacuate the remaining oxygen in the oxygen pipeline, protect the oxygen pressure gauge when the secondary reducer sudden malfunction and the oxygen gauge hand ultra time K144 limit. 10. The problem in the compressed air used as the The research on treating hypoxic ischemic compression medium. The air pressure cabin uses the encephalopathy by hyperbaric oxygen treatment and compressed air to take the compression medium when the changes of cytokines treat patient, its quality is related with patient's safety and Zhou Yihong, Chan Guiyang, Tang Lanfen, Ao Dang the effect of the treatment directly. In medical oxygen Department of Pediatrics, Affiliated Hospital of Guang cabin GB code stipulated the compressed air must store Dong Medical College, Zhanjiang, China up period of time after the preparation to be able to use, but in fact can achieve the oxygen cabin is few. In my Objective: To investigate the changes of serum humble opinion, the compressed air used in the oxygen Interleukin-6(IL-6), Tumor necrosis factor-α (TNF-α) and cabin should refer to the standard of the military symbol Insulin-like growth factor-1 (IGF-1) in hypoxic ischemic "Diving Breath Gas" to carry out to maintain patient's encephalopathy(HIE) by hyperbaric oxygen treatment treatment benefit. The first choice of vents one's anger (HBOT) and clinical significance. Methods: Fourty-eight with air pressure is non-oil air compressor. Install the heat neonates with HIE were divided into two groups. Routine interchanger behind the air outlet of the compressor, treatment group were intervened with the conventional which cause the compressed air temperature prepared treatment, hyperbaric oxygenation group in addition to the dropped greatly and reduce the presence of hydrocarbon conventional treatment. Serum IL-6, TNF-α and IGF-1 in in the compressed air. Install the multistage fluids behind 48 cases of HIE were detected by radio-immunity. Result: the export mouth in the heat interchanger separator to The increaseed range of serum IL-6 in HBOT group were reduce the hydrocarbon again. Conditional liquid oxygen significantly higher than routine therapy group (P < 0. 05); chamber should increase behind the gas separator with a the decreased range of serum TNF-α and IGF-1 in HBOT dedicated dry precision porous filter so that the group were significantly higher than routine therapy group preparation of the compressed air would be a better (P < 0. 05). Conclusion: HBOT could increase the quality. 11. The selection of the activated carbon in production of IL-6 and reduce the production of TNF-α oxygen chamber. The round circle columnar husk should and IGF-1 . HBOT is an effective therapy for hypoxic and be the first choice of the activated carbon in oxygen ischemic brain damage. chamber supply air filters, because the graphite activated carbon is refined from the coal and the coal-tar oil, but 0.3MPa the air feed limiting pressure can run out the micro granulated activated carbon from the air strainer, K145 Hyperbaric oxygen therapy adhesive effect of 114 in 7 days, fear reduced earlier (P<0.05). Conclusion: cases of obstruction HBO treat MS is one of curative effect at present. Yubao Zhang Lei Ge Shumin Meng Wen Zhai Fuqin Wang The Frist Peoples Hospital of Harbin, Ha’erbin, China K148 Objective: To investigate the high-pressure oxygen (HBO) Observation on the curative effect of HBO on treating for the effect of adhesive intestinal obstruction. Methods: continuing vegetative state 114 cases of postoperative adhesions with obstruction Xuming Zhou, Haiying Qiu, Qun Cai, Huiting Cong, were randomly divided into two groups, the control group Ji Wang using conventional drug treatment. HBO group, in addition The Frist Hospital of Ha’erbin, China to conventional drugs, the daily 0.25 MPa pressure, oxygen absorption 60 minutes (10 minutes to break the Purpose: Detection HBO curative effect observation of middle), a total of 20 days. Results: 20 days later, HBO treat continuing vegetative state (CVS). Method: Random group cure rate significantly higher than that, there was a sample divided 2 groups of HBO treatment and significant difference, statistical significance. Conclusion: comparison. Except HBO group routine medication The high-pressure oxygen therapy can increase the treatment, there are HBO pressure 0.25mpa and absorb intestinal wall of the blood supply adequate oxygen supply, pure oxygen 60 mins (break 10 mins), once per day, total accelerate intestinal peristalsis, and promote the 20-70 times. Comparison group routine medication absorption of inflammatory mediators, in the treatment of treatment. Result: Curative effect of HBO group adhesive obstruction plays a role should not be surpassed comparison group (P<0.01), which is early and underestimated, is an effective treatment measures. long period of treatment are better curative effect. Conclusion: HBO which is importance of CVS routine treatment.

K146 Hyperbaric oxygen chamber, hospital infection control K149 Haiying Qiu, Yan Zhao, Ji Wang, Lihua Liu, Rui Li Treating 180 cases of paroxysmal deafness by using The Frist Peoples Hospital of Harbin, Ha’erbin, China HBO combined with acusector Xuming Zhou, Shumin Meng, Lei Ge, Bo Zhai, Bo Dong Objective: To eliminate nosocomial infections, control of The Frist Hospital of Ha’erbin, China high-pressure oxygen chamber and ancillary devices colonies of bacteria to ensure that the hyperbaric oxygen Purpose: Discussion HBO result of HBO combines therapy effective. Method: Siebenpfeiffer disinfectants abusector which treats paroxysmal deafness (PD) and ultraviolet light to the oxygen chamber of the air, Method: Random sample there are 2 groups which 180 oxygen subsidiary devices were disinfected. Results: air PD HBO and comparison. Group HBO: Except routine control colonies in ≤500cfu/M3, articles colonies in the treat with acusector and medication. Meanwhile, there are control of ≤10cfu/M3, put an end to the oxygen chamber HBO pressure 0.25mpa and absorb pure oxygen 60 mins treatment in hospital infection. Conclusion: The weekly (break 10 mins), once per day, total 10-30 times. Group regular disinfection of the patient and reasonable comparison routine treat with acusector and arrangements for a special event, special disinfection, to medication.Meanwhile, sickness in a week and over a put an end to high-pressure oxygen treatment of hospital week treat with HBO and comparison with PD under infection. treatment of routine acusector and medication. Result: Group HBO for PD curative effect rate and getting better rate and more than over a week obviously (P<0.01). Group in a week curative effect rate and getting better rate K147 are more than over a week HBO treatment group(P<0.05). HBO treat meniere syndrome 66 cases Diversity exist prominence and significance of statistics. Danhong Song 1, Wen Zhai 2, Pei Liu 2, Xiaohua Li 2, Conclusion: HBO combine acusector and medication Hong Li 2 which treat PD are better than simple acusector and 1. The second Peoples Hospital of Harbin, Ha’erbin, medication obviously. Meanwhile earlier treatment is China better for curative effect. 2. The Frist Peoples Hospital of Harbin, Ha’erbin, China

Purpose: Detection HBO treats Meniere Syndrome (MS) K150 clinic curative effect. Method: There are 2 groups, 66 Hyperbaric oxygen treating 32 cases of metal cases. Except HBO group routine medication of dilatated poisoning blood vessels, there are HBO pressure 0.20 mpa and Li Wen, Zhang ZhiGe, Tuo LiuYing, Yang Meiping, Shi absorb pure oxygen 60 mins (break 10 mins), once per Zhongheng day, total 15 times. Comparison group adopt routine Department of Hyperbaric Oxygen, Jiangbin Hospital of medication of dilatated blood vessels. Result: HBO treat Guangxi MS total efficient rate 95.8%.Comparing with comparison group, diversity is obviously (P<0.05). Enforcing position We applicated hyperbaric oxygen (HBO) treatment in 32 legs caused a long delay, had infected and fever. After 26 cases of metal poisoning in November 1996 to December times HBOT, the wounds were recovered.The treatment 2007, and compared to similar cases who received principls of scald by HBO were discussed in this report. conventional treatment in November 1987 to November 1996, there was significant difference, it is report as follows: Clinical data: 1. General information: There were 63 cases, 56 cases from the production workers, 9 cases K152 of mistaken consumption poisoning. 32 cases of HBO The clinical application of a new oxygen treatment group, 20 cases men, 12 women, aged 22-55 inhalation-exsufflation device with emergency years old, of which 9 cases of lead poisoning, 12 cases of intra-chamber atomization and humidification manganese poisoning, 9 cases of tin poisoning, 2 cases Youcun Wang, Peizhen Shi, Jianwei Xu, Zengshan Liu of arsenic poisoning. 31 cases of control group 19 men, The department of hyperbaric oxygen, General Hospital of 12 women, aged 20-56 years old, of which 9 cases of lead Jinan military area, Jinan, China poisoning, 11 cases of manganese poisoning, tin poisoning in 10 cases, 1 case of arsenic poisoning. Two The manufacture and clinical application of a new oxygen groups had almost the same performance. 2. Clinical inhalation-exsufflation device with emergency performance: there are different levels of intra-chamber atomization and humidification were unconsciousness in 12 cases and the rest had the reported. Without any defect that the traditional device performance of headache, dizziness, fatigue, insomnia, has, the new device was work-reliable and nausea, vomiting, tremor, polyuria and so on. 3. simple-operated. The application of the new device in Treatment: two groups used metal driving, symptomatic hyperbaric oxygen chamber increased the methods of treatment and support treatment. HBO treatment group intra-chamber emergency treatment, the indications and used medium-sized hyperbaric oxygen chamber, the rescue capability of the chamber, as well as improved compressing air to 0.2 MPa, inhaling oxygen for 80 the hygienic criterion of intra-chamber oxygen-supply and minutes, the middle rest for 5-10 minutes,once a day, 12 the effects of treatments. It could also eliminate the times was 1 course, general 2-3 courses, up to 5 courses. hidden danger of accidents. 4. Efficacy evaluation standard: full recovery: consciousness and other symptoms are back to normal. markedly effective: consciousness has returned to normal, but the signs have not been completely back to K153 normal. improveing: symptoms and signs have got better A case of using hyperbaric oxygen to treat mathenol slightly after treatment. invalid: signs and symptoms did poisoning not change after treatment. Results: effect of HBO Yalin Wang, Xiujie Li, Rong Li treatment group were: 11 cases full recovery, 11 cases Dalian Center Hospital, Dalian, China markedly effective, 7 cases improved, 3 cases invalid, and the total efficiency was 90.6 percent. Control group: 5 A patient, male, 40 years old, suffered from sudden cases full recovery, 8 cases markedly effective, 9 cases serious stomach ache for 2 hours during working, improved, 9 cases invalid, the total efficiency was 70.9 accompanied by nausea and vomiting. He touched percent. There was significant difference in two groups, P methanol in working environment. Preliminary diagnosis: <0.05. Discuss: the mechanism of HBO treatment in the stomach ache requires further examination. Two metal poisoning is to rapidly improve the state of hypoxia, hours later, the illness severity increased. His awareness to interrupt the vicious cycle of edema, so it can effectively became unclear, accompanied by breathing difficulty, limb alleviate symptoms. HBO can increase blood oxygen getting cold, cyanosis, systemic tinea versicolor, no urine, pressure, improve the metabolism of brain cells, reverse and BP 90/60 mmHg. Examination in laboratory shows: K or delay the degeneration of brain cells caused by the 5.63mmol/L, BUN 12mmol/L, Cr 315umol/L, artery blood poisoning, maintain the flexibility and toughness of red Pa CO 213mmHg, PH6.8. Diagnosis: methanol poisoning, blood cells and reduce the occurrence of hemolysis. This acute renal failure, metabolic acidosis, hyperkalemia, and observation of HBO treatment in metal poisoning had distributed shock. The treatments to the patient include quite satisfactory results. So for the patients of metal orotracheal intubation and mechanical ventilation, poisoning,we suggest take early HBO treatment on the puncturing administration to right internal jugular vein, and basis of metal driving and symptomatic treatment, hemofiltration. 24 hours later, the patient’s awareness patients of chronic poisoning should increase more recovered, the amount of urine returned to the normal courses appropriately. level, and he could make conversations. We stopped mechanical ventilation. The patient said that he could not see objects. Ophthalmology consultation: his eyes had no light perception; the pupils dilated, D=5mm; direct and K151 indirect light reflex were both weak. We use energy Treating a case of scald by HBO mixture, neurotrophic drugs to improve microcirculation Haiping Yang, Juan Chang, Chuanying Sun and use Hyperbaric Oxygen (HBO) therapy. 48 hours Youanmen Hospital of Beijing hospitail association, after the patient entered the hospital, we performed HBO Beijing, China therapy. Treatment Program: surface pressure 0.12Mpa, oxygen inspiring time 60min, once per day. 32 days after This article reported a case of scald by boil water, who is a the patient received HBO treatment, his eye sight diabetic woman, was treated by HBO. The wounds of her recovered completely. Discussion: The patient is a typical patient suffering from methanol poisoning, the diagnosis is clear, and the HBO therapy is effective. The purposes of using hyperbaric oxygen therapy in the K155 treatment include: 1. HBO helps to improve the activity of The effect of hyperbaric oxygen treatment on the cytochrome oxidase, to inhibit the generation of formic plasm concentration of tPA, PAI-1 in the patients of acid and lactic acid, and to ease the acidosis. 2. HBO has ischemic strok positive effect to repair and regenerate damaged nerves, Wei Wang 1, Lin Yang 2, Chunjin Gao 2, Baosen Pang 2 and helps to recover the function of optical nerves. 3. 1. Department of Hyperbaric Oxygen, The Third Hospital HBO accelerates the regeneration of damaged capillaries, of Hebei Medical University, Shijiazhuang, China provides oxygen for the establishment of circulation in 2. Department of Hyperbaric Oxygen, Beijing Chao Yang branch retina, improves the hypoxia status of the fundus Hospital, Capital University of Medical Sciences, Beijing, tissue, and prevents pathological changes of inner cells of China retina caused by ischemic and hypoxia. 4. The formic acid generated during the methanol metabolism is also toxic to Objective: To investigate the effect of hyperbaric oxygen the retina. Hyperbaric oxygen may enhance the activity of treatment on the plasm concentration of tPA, PAI-1in the formyl-H4 folate dehydrogenase, further promote the patients of ischemic stroke. Methods: 137 patients with metabolism of formal acid. On the whole, the patients acute ischemic stroke were divided randomly into routine suffering from harmful gas poisoning should be treated therapy group (routine group), hyperbaric oxygen therapy with HBO as early as possible. And HBO is one of the group (HBO group). There were 27 healthy volunteers in necessary treatments to cope with harmful gas poisoning. control group (control group). We measured the plasm concentration of tPA, PAI-1 respectively in the patients with ischemic stroke on the 1 st and 11 th day after admission. Results: On the 1st day, in the patients of the K154 HBO group or of the routine group, the plasm A therapy effect analysis of hyperbaric oxygen concentration of tPA and PAI-1 were significantly higher combination with method of blood-activating and than that of the control group (P>0.05); The tPA collateral-unblocking in treating patients with acute concentration was lower in the HBO group compared with cerebral infarction the routine group (PAfter the routine treatment, the plasm Wang Xiaohong concentration of tPA decreased (P<0.01); After HBO Department of Rehabilitation and Physiotherapy,Wuhan treatment, the plasm concentration of tPA, PAI-1 both General Hospital of Guangzhou Command, Wuhan, decreased (P<0.01). Conclusion: In the patients of acute China ischemic stroke, hyperbaric oxygen treatment can decrease the plasm level of tPA, PAI-1. Thus hyperbaric Objective: To investigate the clinical effects of hyperbaric oxygen treatment can effectively improve the function of oxygen (HBO) combination with method of coagulation-fibrinolytic system in the patients with blood-activating and collateral-unblocking (BACU) on ischemic stroke, and may ameliorate therapeutic effect. patients with acute cerebral infarction (ACI) and to analyze the mechanism of action. Methods: 84patients suffered from ACI were randomly divided into HBO plus BACU therapy group (HBO group) and BACU therapy K156 group (BACU group). The levels of serum endothelin Clinical effective observation on combined hyperbaric (ET-1), nitric oxide (NO) and the C-reactive protein (CRP) oxygen therapy for noxious gas poisoning were measured before and after treatment. The resultant Shuzhen Wang, Yuqin Han, Yun Li, Hongling Yang, data were compared with normal healthy crowd. Results: Jianming Zhan The levels of serum ET-1 and NO in patients with ACI Qilu Hospital of Shan Dong University, Jinan, China obviously elevated (P ＜0.01). After treatment, the serum Objective: To investigate the effect of ET-1 decreased, NO enhanced, and the ratio of ET-1/NO combined hyperbaric oxygen therapy on noxious gas decreased, while the decrease degree of the HBO group (mainly on carbon monoxide) poisoning. Methods: 339 was more remarkable, compared with the BACU group (P cases of carbon monoxide poisoning were treated by ＜0.01)At the same time, the CRP level in HBO group was hyperbaric oxygen, with the supplement of the medicine low significantly, compared with those before treatment (P (vasodilator, diuretic, neurotrophic medicine). On the base ＜0.01), and no obvious change were observed in CRP treatment of air compression, patients were treated with 0.2 Mpa hyperbaric oxygenation (severe patients at 0.25 levels in patients before and after BACU therapy (P＞ Mpa), wearing masks and inhaling pure oxygen for 20 min 0.05). Conclusions: Compared with solitary drug therapy, and the air in chamber was inhaled for 5 min, repeating 3 HBO combination with BACU had better effect on ACI. times such inhalation, then decompressed. Twice HBO The main mechanisms of above combined therapy shows therapy a day for severe patients, once a day after the that it could improve of vascular endothelium function, patients awake. For 10 time HBO as a period of treatment. inhibit the formation of ET-1, relieve vasospasm, Results: 325 cases were cured, decrease in vivo inflammatory reaction, restrain blood becoming life-independent, 8 cases dead because of clotting, promote the establishment of collateral circulation, failure to receive HBO treatment due to critical condition and prevent ischemic brain tissue damage. and transient vital signs, and 6 cases of carbon monoxide intoxication delayed encephalopathy. The total effective rate is 95.8%. Conclusions: The combined hyperbaric than those of from the control group when hospitalized therapy for noxious gases poisoning is effective, with a (P<0.01, P<0.05). D-D kept increasing on the 10 th day, high cure rate, quick effect, less complication and low after admission (P<0.01); while Fg was lower than that of mortality rate. the cortrol group (P<0.01). On the 20thday, D-D started to decrease. However, it was still higher than normal level (P<0.05); while Fg becomed normal, which showed no significant difference from that in the control group K157 (P>0.05); D-D and Fg levels in the blood plasma of the Therapeutic effect of hyperbaric oxygen integrated HBO group were higher than those of the control group therapy on hydrocephalus of craniocerebral injury when hospitalized (P<0.01,P<0.05). D-D kept increasing Shengzhi Wang, Yu Peng on the 10thday after admission (P<0.01). However, it was Binhai Hospital, Shengli Oil Administration, Dongying, still lower than that of the routine treatment group Shandong, China (P<0.05); while Fg started to decrease, but it was higher than normal level (P<0.05). On the 20th day, D-D and Fg Objective: To explore the curative effective of hyperbaric levels showed no significant difference from those of the oxygenation (HBO) combined with drugs therapy on control group (P>0.05). Conclusions: HBO can relieve hydrocephalus of craniocerebral injury. Methods: Ten coagulation, anticoagulation and abnormal fribrinolysis pentients with hydrocephalus from craniocerebral injury due to ischemia and damage in the vessels through were treated with hyperbaric oxygen treatment which regulation of D-D and Fg levels in blood plasma, which given 0.23 MPa HBO exposure ,20 min × 4( oxygen ) + 5 was effective in preventing treating and ischemic min (air ) once daily for 1~6 courses all together and once apoplexy. course was 10 days, basides drug`s treatment of citicoline sodium injection and adenosine disodium triphosphate injection al. to observe and evaluate the clinical changes and hydrocephalus change on CT. Results: Ten K159 pentients with hydrocephalus from craniocerebral injury Effect of hyperbaric oxygen on iron deficiency in improved significantly clinical symptoms and signs after patients with sensorineural deafiness 3~10 days, the patients clinical symptoms ,signs and qiusha wang 1, shuchang tian 2, aihua sun 2, hydrocephalus on CT almostly disappeard after 30 days baodong wang 2, fuqiong pan 3, haian yu 1, shizhong wang 1, `treatment. Some pentients went on treating for 60 days xuejun sun 4 and all cured. Conclusions: Hyperbaric oxygen 1. Department of Hyperbaric Oxygen, the 2nd People’s integrated therapy may promotly repair the circulatory Hospital of Changzhou, Affiliated Hospital of Nanjing system of cerebrospinal fluid in craniocerebral injury`s Medical University, China pentients and hydrocephalus were gradually insorpted or 2. Department of Otorhinolaryngology, Shanghai got into the circulatory system of cerebrospinal fluid, thus Changzheng Hospital, China controled hydrocephalus growth. It must be treated the 3. Department of Medical Rehabilitation, the Peoples pentients on hydrocephalus of craniocerebral injury with Hospital of Sichuan Province, China HBO at the right time, in order to increase the therapeutic 4. Department of Nautical Medicine, Faculty 0f Navy effect. Medicine, Second Military Medical University, Shanghai, China

Objective: To evaluate the effect of hyperbaric oxygen K158 (HBO) therapy on iron defieiency in patients with The effect of hyperharic oxygen on D-dimer and sensorineural deafness. Methods: Two hundred and fifty fibrinogen in the blood plasma of ischemic apoplexy three patients were randomly selecled from outpatients patients and inpatients with sensorineural deafness and treated Qiusha Wang 1, Shizhong Wang 1, Chunjin Gao 2, with HBO therapy from Dec. 1996 to Dec. 2004. Serum Qihua Yang 1, Jin Mei 1, Ping Zhang 1, Jianding Gu 1 ferritin (SI) and hemoglobin (Hb) were measured before 1. Department of HBO, The 2nd People’s Hospital of treatment and after one and two courses of treatment. Hb Changzhou, Affiliated Hospital of Nanjing Medical and SI were also measured in 250 people undergone University, Changzhou, China, healthy check-up in 2005. Results: After two courses of 2. Departmen of Hyperbaric Medicine, Beijing Chaoyang HBO therapy, better curative effect was obviously shown Hospital, Beijing, China in treating mild deafnes than in treating moderaie deafness. Statistically significant difference in Hb and SI Objective: To observe the effect of hyperbaric oxygen was observed before treatlment and after one course of (HBO) on D-dimer (D-D) and fibrinogen (Fg) in the blood treatment, also after one course of treatment and after two plasma of ischemic apoplexy patients. Methods: courses of treatment, respectively ( P<0.05, P <0.01). Ischemic apoplexy patients were randomly divided into There was no statistically significant difference in Hb routine treatment group (97 cases), HBO treatment group between patients after two courses of treatment and (137 cases), and healthy control group (40 cases) was healthy people ( P>0.05), but there was a statistically setup. D-D and Fg levels in blood plasma were detected significant difference in SI between thern ( P<0.05). by coagulometer of the STAGO Company and its Conclusions: Hyperbaric oxygen therapy may adjust SI corresponding kit. Results: D-D and Fg levels in the and Hb in patients with sensorineural deafness. blood plasma of the routine treatment group were higher state for more 60 days. The excellence rate and the improvement rate were significantly higher in cases below K160 30 years than these in cases over 30 years. Conclusion: Application of the apparatus for eustachian tube The effectiveness of hyperbaric oxygen on the patients opening function (ETOF) measurement before the with persistent vegetative state caused by cerebral hyperbaricoxygenation (HBO) trauma and the patients who just begin to receive hyperbaric oxygen therapy treatment when having been ill Qiusha Wang 1, Chunjin Gao 2, Peisong Wang 3, for less 61 days is better. The effectiveness of therapy is 4 4 1 Yunjing Wang , Yuming Yang , Yuhong Zhang , better in younger cases than that in old cases 。 Guoying Wu 1, Yi Zhou 1, Jing Cao 1

1. Department of Hyperbaric Oxygen, the 2nd People’s

Hospital of Changzhou, Affiliated Hospital of Nanjing

Medical University, China K162 2. Department of Hyperbaric Oxygen, Beijing Chaoyang Effect of hyperbaric oxygen on ultrastructure of liver Haospital, Capital University of Medical Science, China and kidney in rats after acute carbon monoxide 3. Department of Hyperbaric Oxygen, Medical school poisoning Hospital Haospital of Qingdao University, China Guozhong Wang, Chunjin Gao, Huan Ge 4. Naval Medical Research Institute of CPLA Shanghai, Department of Hyperbaric Oxygen, Beijing Chaoyang China Hospital, Capital University of Medical Sciences, Beijing,

China Objective: To monitor Eustachian tube opening function with the application of measurement of sound conduction Objective: Acute carbon monoxide poisoning (ACMP) is before HBO. Methods: Eustachian tube opening function likely to be the most common cause of poisoning were monitored in patients before HBO with a worldwide and often results in persistent damage to the newapparatus for ETOF measurement, model HY-100 important organs such as brain, liver and kidney. Some applied with high frequency sound at 7KHZ.If at the first trials have definitively shown hyperbaric oxygen (HBO) as time the patient’s Eustachian tube was not open, the the efficacious therapy for acute carbon monoxide measurement would be done in the next time after actions poisoning. The objectives of this article are to study the of swallow, Valsalva maneuver and mandible oscillation protective effect of hyperbaric oxygen on had been did. The results of measurement were the ultrastructure of liver and kidney in rats after acute classificated by four grades. The standard for HBO was carbon monoxide Poisoning. Methods: The rats were made by the grade and whether Eustachian tube is open. randomly divided into ACMP 1st day group, ACMP 5th Results: The patient’ Eustachian tube with grade four in day group, ACMP 10th day group, ACMP15th day classification and failure in five times of continues action group,HBO1st day group, HBO5th day group, HBO10th during the measurement was regarded as it was not open day group, HBO15th day group and normal control group. and the patient was not suitable for HBO. Conclusion: Model rats after acute carbon monoxide poisoning were There is an advanced, stable and reliable performance in established. The ultrastructure changes of liver and the newapparatus for ETOF measurement, themonitor kidney in rats of all groups were observed by electron and classification of Eustachian tube opening function microscope. Results: Slight mitochondria swelling, focal prevents from barotrauma and keeps HBO in going well. vacant area and rupture of mitochondria crista was

observed in some liver cells of ACMP 1st day group. The

pathological changes became aggravating in liver cells of

ACMP 5th day group and ACMP 10th day group, and K161 alleviating in liver cells of ACMP 15th day group. Effect of hyperbaric oxygen combined therapy on Compared with simultaneous ACMP group, the pathologic persistent vegetative state in 46 patients changes of ultrastructure in liver cells of HBO groups were Wang Min significantly improved. From ACMP 1st day group to Department of Rehabilitation of Longgang District Central ACMP 5th day group, the pathologic changes of Hospital of Shenzhen, Shenzhen, China ultrastructure in kidney cells was ingravescent. Although

the pathologic changes of ACMP 10th day group and Objective: hyperbaric oxygen combined therapy on ACMP 15th day group became alleviating, slight persistent vegetative state and explore the best mitochondria swelling was observed in some epithelial therapeutic regimen for it. Methods: Forty-six cases of cells, meantime interstitial edema and congestion persistent vegetative state treated with hyperbaric oxygen remained obvious. Compared with simultaneous ACMP combined therapy from 1995 to 2006 were analyzed group, the pathologic changes of renal glomerulus and retrospectively, including the effects of etiological factors tubule of HBO 1st group and HBO 5th group were and age factors and treatment juncture on therapy. Result: significantly improved. Slight mitochondria swelling was The excellence rate and the improvement rate were observed in a few renal tubular epithelial cell of HBO10th significantly higher in cerebral trauma group than those in day group and HBO15th day group. Conclusion: Carbon no cerebral trauma group. And two groups in which the monoxide has the toxic effects of tissue hypoxia and patients began to receive treatment when became produces various systemic and neurological persistent vegetative state for less 61 days recovered complications. Early hyperbaric oxygen can alleviate better than the other one in which the patients began to pathologic lesion of ultrastructure of liver and kidney in receive treatment when became persistent vegetative rats after acute carbon monoxide poisoning, which have clinical therapy effectually , the curative effect of HBO significant protective effect on the liver and kidney. have been assumed.

K163 K165 Empiricad study about the expression of IGF-1 on Study of the medical security on the 140m heliox brain tissue injured by treatment of hyperbaric saturation–166m excurssion diving oxygenation in rats after traumatic brain injury Qinglin Lian, Xuejun Sun Jinzhong Teng, Jianfeng Wen Department of Diving Medicine, Second Military Medical The 94th Hospital of PLA, Nanchang, China University, Shanghai, China

Objective: To investigate the relationship between the Objective: In order to raise the salvageability of the naval, therapeutic effect of hyperbaric oxygenation on brain cultivate the divers of the saturation deep diving and injury and the cerebral expression of IGF-1. Methods: cultivate the person of medical security and diving Base upon the Feeney’s model, we set up the cerebral equipment support. Methods: eight divers were exposed trauma model in SD rats. The rats were randomly to 140m He-O2 saturation pressure in the DCC for 48h, assigned to four groups: sham operated group, Control, the partial pressure of O 2 was 40±2 kPa in the DCC. trauma and normal pressure oxygenation treatment (NPO) During the saturation period, the divers the excursion group, trauma and hyperbaric oxygenation treatment dives reached to a depth of 164 ～166m by means of the (HBO). The cerebral expressions of IGF-1 were analyzed SCC and effectively finished the work on the bottom. The with immunohistochemical technique. Results: After the divers safely return normal pressure after 149 h 20 min treatment of hyperbaric oxygen, the expression of IGF-1 decompression. Base on the clinical examination, the in hyperbaric oxygen treatment group was higher than function of nerve, mentality, heart and blood vessels, normal pressure oxygen treatment group and surgical respiration, immunity, stress, motion balance and audition trauma control group (P<0.01). Conclusion: The of divers were tested in different phases. Results: At the expression of IGF-1 enhanced in nerve cells after brain saturation-excursion period, worry, anxiety and injury, and it enhanced more obviously under hyperbaric depression of the divers were existed, the MVV of oxygen treatment. It indicates that the treatment of pulmonary functions were significantly lower with the hyperbaric oxygen can protect and recover the impaired diving depth, the accurate rate of the ball put test nerve cells. Improved the expression of IGF-1 in brain decreased sharply, the levels of 17-OHCS, CA, A, NA, tissue cells might was one of the nerves protection ways. ACTH, FSH, T, GH, and C were significantly higher.

Conclusions: In this successful diving, the medical

security was very effective and practical. The

physiological changes of the body were temporary and K164 may be recovered. Attendant experience of combination therapy containing hyperbaric oxygen for fetal growth restriction induced by severe preeclampsia

Yuhua Wang, Hongjian Liu K166 The people’s hospital of Cangzhou, Hebei, China Mechanism of hyperbaric oxygen preconditioning in

neonatal hypoxia-ischemia rat model Objective: To explore the attendant means of Li Zhang, Wenwu Liu, Zhimin Kang, Xuejun Sun combination therapy containing hyperbaric oxygen(HBO) Department of Diving Medicine, Faculty of Naval Medicine, for fetal growth restriction induced by severe Second Military Medical University, Shanghai, China preeclampsia. Methods: 32 patients with fetal growth retardation(FGR) induced by severe preeclampsia, Objective : We examined the mechanism of hyperbaric treated by HBO combined with routive methods .treatment oxygen preconditioning (HBO-PC) in neonatal HI rat Pressure was 0.15 Mpa,Qd; Continnal therapy times is model. Methods : Seven-day-old rat pups were subjected five. Results: in all 32 patients, the prominent effective to left common carotid artery ligation and hypoxia (8% ratio was 59.4%, the effective ratio was 100%, and the oxygen at 37ºC ) for 90 min. HBO (100% O , 2.5 existent ratio of newborn baby was 94.1%. By 2 atmospheres absolute for 2.5 h) were administered by strengthening psychology nursing and special nursing of placing pups in a chamber 24 h before HI insult. Brain the boodle pregnant woman, no one side effect was found. injury was assessed by the survival rate, 2, 3, Oxygen –toxicosis were not discovered in the newborn 5-triphenyltetrazolium chloride (TTC), Nissl, TUNEL babies observed two years later. Conclusions: By straining and caspase-3,caspase-9 activities after HI. improving blood oxygen-content and blood oxygen Results : In HBO preconditioned animals, survival rate dispersive capability, so the placental microcirculation was increased, infarct ratio was decreased, and the improved significantly, lacking oxygen of fetus positive stained TUNEL cells was reduced, accompanied corrected ,the live rate of newborn babies improved by and suppression of caspase-3 and -9 activities. significantly. By mentality nursing timely and effective and Conclusion : These results indicate that a single HBO-PC nursing in oxygen-store meticulously and attentively, the appears to provide brain protection against HI insult via HBO treatments to the boodle of pregnant women were inhibition of neuronal apoptosis pathways. accomplished smoothly. Supporting the carry out of hyperbaric oxygen therapy group, 90 patients were treated with hyperbolic oxygenation one time in ten days besides the medicine treatment; Results : The affect of K167 hyperbaric oxygen therapy group on the cerebral Hyperbaric oxygen pretreatment accelerates infarction was more than the medicine treatment group; angiogenesis and alleviates myocardial ischemia in Conclusion : hyperbaric oxygen therapy could disease rats the side effect of nerve damage, and prevent the recurrent Cuihong Han 1, Li Lin 1, Qiang Sun 1, John H. Zhang 2, of the cerebral infarction. Xuejun Sun 1 1. Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, 200433, P. R. China. K169 2. Department of Physiology and Pharmacology, Loma Hyperbaric oxygen preconditioning promotes Linda University School of Medicine, Loma Linda, angiogenesis in rat liver after partial hepatectomy California, USA Ping Ren 1, Zhimin Kang 2, Guojun Gu 2, Yun Liu 2, Weigang Xu 2, Hengyi Tao 2, John Zhang 3, Xuejun Sun 2 Objective : Hyperbaric oxygen (HBO) has been previously 1. Department of pharmacology, Chinese Pharmaceutical shown to protect the heart from ischemia-reperfusion University, Nanjing, China injury. The present study aimed to test the hypothesis that 2. Department of Diving Medicine, Faculty of Naval HBO pretreatment enhances neovascularization in Medicine, Second Military Medical University, Shanghai, ischemic myocardium. Methods : Adult male China Sprague-Dawley rats were randomly divided into four 3. Department of Physiology and Pharmacology, Loma groups: normoxia + sham surgery (CS), normoxia + Linda University School of Medicine, Loma Linda, permanent occlusion of the left anterior descending (LAD) California, USA coronary artery (CMI), HBO pretreatment + sham surgery (HS), HBO pretreatment + permanent LAD occlusion Objective : Hyperbaric oxygen preconditioning (HBO-PC) (HMI). Rats receiving HBO pretreatment were increases the level of HIF-1α and its target gene VEGF intermittently exposed to 100% O 2 at 2.5 atmospheres which is involved in angiogenesis. Liver regeneration is an absolute (ATA) for 60min, twice daily for 2 days followed angiogenesis-dependent process. We hypothesized that by 12h of recovery in room air prior to the myocardial HIF-1α and VEGF mediated the angiogenesis effect of ischemic insult induced by LAD ligation. Rats in the HBO-PC on regenerating rat liver. Methods : Male normoxia group were time matched with the HBO group Sprague Dawley rats received HBO-PC followed by 70% and maintained under normoxic conditions prior to LAD partial hepatectomy. Proliferation of hepatocytes and occlusion. At 3 and 7 days after LAD occlusion, heart endothelial cells was evaluated by BrdU staining. function parameters were measured by inserting a Microvascular density was assessed by catheter into the left ventricle, infarct size was calculated immunohistochemistry. mRNA expression of HIF-1α was using the method of TTC staining, myocardial capillary assessed by quantitative RT-PCR and protein levels of density was determined by immunohistochemical staining HIF-1α and VEGF were assessed by western blot. HIF-1α with a monoclonal anti-CD 31 /PECAM-1 antibody, VEGF DNA-binding activity was determined with an protein level was determined by Western blot analysis, ELISA-based kit. Results : HBO-PC increased the and cell proliferation was detected by BrdU staining. proliferation index of endothelial cells and microvascular Results: At 3 days after LAD ligation, the infarct size of the density at 48 hr after partial hepatectomy. The protein HMI group was significantly smaller than that of the CMI level and DNA-binding activity of HIF-1α and the protein group (26±2.5% vs. 38±3%, P max and -dP/dt max were level of VEGF were increased by HBO-PC before and significantly improved in the HMI group compared to the after partial hepatectomy. Partial hepatectomy alone also CMI group at 3 and 7 days after LAD occlusion. Capillary increased proliferation index and the expressions of density, VEGF protein levels, and cell proliferation HIF-1α and VEGF. Conclutions : Our results indicated detected by BrdU staining were significantly increased in that the angiogenesis effect of HBO-PC on liver after the ischemic myocardium pre-exposed to HBO. partial hepatectomy could be achieved by increased Conclusion : HBO pretreatment accelerates angiogenesis HIF-1α activity and VEGF expression. However, the and alleviates myocardial ischemia in the present rat angiogenic effect of HBO-PC is moderate and HBO-PC model. failed to produce additional effect on the enhancement of HIF-1α and VEGF induced by partial hepatectomy alone.

K168 The affect of hyperbaric oxygen therapy on the K170 cerebral infarction Hydrogen therapy reduces apoptosis in neonatal Wenli Zhang 1, Xiaojie Ding 2, Wei Wang 2 hypoxia-ischemia rat model 1. Thoracic hospital of Qingdao, China Jianmei Cai, Zhimin Kang, WenWu Liu, Xuejun Sun 2. Central hospital of Qingdao, China Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China Objective : To study the affect of hyperbaric oxygen therapy on the cerebral infarction; Methods : In the Objective: Hydrogen gas has been used in medical applications to prevent decompression sickness in deep It reported eighteen cases of operating cerebral anabiosis divers from safety profiles. Recently, Ohsawa et al found under hyperbaric oxygen after cardiopulmonary anabiosis that molecular hydrogen can selectively reduce •OH and success and discussed the factor of success. There are ONOO– in cell-free systems and exert a therapeutic fourteen cases of cerebral anabiosis success, two cases antioxidant activity, using a rat middle cerebral artery of bequeathing persistent vegetative state, two cases of occlusion model. But the mechanism involved in the death. The result of the treatise prompts that the operating protective effects of Hydrogen therapy was unclear. of hyperbaric oxygen therapy on early stage basing the Hypoxia-ischemia brain injury is a major cause of cardiopulmonary anabiosis success can improve the neuronal cell death especially apoptosis in the perinatal success rate of the cerebral anabiosis. period. This study was designated to examine the effect of hydrogen therapy on apoptosis in an established neonatal Hypoxia-ischemia rat pup model. Methods: Seven-day-old rat pups were subjected to left common K173 carotid artery ligation and then 90 min hypoxia (8% Psychological problems of the patients in hyperbaric oxygen at 37 °C). Immediately after Hypoxia-ischemi a chamber and nursing methods insult, pups were placed into a chamber filled with 2% Song Juan Hydrogen for 30 min, 60 min, or 120 min, respectively. 24 the 306th Hospital of P.L.A, Beijing, China hr after 2% Hydrogen therapy, the pups were decapitated and brain injury was assessed by Objective: This thesis mainly studies the psychological 2,3,5-triphenyltetrazoliumchloride (TTC), Nissl, and problems of the patients who are treated in the hyperbaric TUNEL staining, as well as caspase-3, caspase-12 chamber, and discuss the nursing methods of appeasing activities in the cortex and hippocampus. Results: the psychological problems of the patients. Methods: Hydrogen treatment in a duration dependent manner Based on the different psychological problems of the significantly reduced the number of positive TUNEL cells patients, we adopt the health education, organizing and suppressed caspase-3 and -12 activities. informal discussions, nursing in the chamber and other Conclusion: these results indicated Hydrogen kinds of measures. Results: Patients objectively get administration after Hypoxia-ischemia appeared to some knowledge of hyperbaric oxygen treatment. They provide brain protection via inhibition of neuronal also actively cooperate the treatment with a very stable apoptosis. mood. Conclusion: Nurses should make a lot of progress in the professional qualities and are aware of the importance of accompanying the patients in the chamber.

K171 Hyperbaric oxygen treatment in nitrogen poisoning Sun Lin, Guan Hongye K174 No.1 People’s Hospital of Foshan Guangdong Province, The detection and clinical significance of NSE level in China cerebral infarction patients’ serum with hyperbaric oxygen treatment Objective : Further understand the importance of Hansheng Sheng, Hongbo Liu hyperbaric oxygen treatment in Nitrogen poisoning. Zhu Hai People’S Hospital, China Actively cooperated with the clinical treatment, hyperbaric oxygen can improve the survival rate. Methods : The This paper is to study serum NSE level in cerebral patient put on the PSA-controlled gas-breathing machine infarction patients with hyperbaric oxygen treatment. The and received treatment in high pressure hyperbaric more NSE, the more it effusing from cells into oxygen cabin. Increase pressure for 25 minutes and cerebrospinal fluid and blood, combined with the changes pressure is 2.3 ATA. Breathing oxygen for 1 hour, then of the cerebral blood flow metabolism, due to cerebral take a 5-minute break and decrease the pressure for 20 edema, which can cause the secondary lesion in century minutes. Result : The patient recovered. Conclusion: nervous system and aggravate the deterioration of the Hyperbaric oxygen is a fast and effective therapy of function of the blood-brain barrier. The NSE level after saving acute hypoxia caused by Nitrogen Poisoning. cerebral infarction if positive related to the pathogenetic condition. Therefor contrasting the NES level after pure oxygen and mixture hyperbaric oxygen treatment can help to predict severity and outcome of cerebral infarction. It K172 provides evidence that Hyperbaric oxygen treatment can The research of the cerebral anabiosis success under improve cerebral anoxia, lower intracranial pressure, hyperbaric oxygen increase the partial pressure of oxygen of reticular Li Sun 1, Yan Shi 1, Guoping Feng 2, Yonggang Tan 3 activating system and brain stem, thus reduce the NES, 1. Hyperbaric Oxygen Department, Shengli Oilfield then promote consciousness recovering, improve the Central Hospital, Shandong, China clinic symptom eventually. 2. Emergency Department, Shengli Oilfield Central Hospital, Shandong, China 3. ICU room, Shengli Oilfield Central Hospital, Shandong, China K175 Clinical value of HBO and drug comprehensive treat that three tasks should be done to improve the treatment in chronic appendicitis level: to prepare oxygen cabin and nursing articles, to Jing-yi SHAO, Yan-bin ZHANG, Zi-yang CAO, nurse carefully, for example: general nursing, oxygen Zhi-hong XIU, Pei-jin Lu inhale, sputum suction and observation on patients illness Liaocheng Second People’s Hospital Affiliated to Taishan and vein transfusion, to improve the level of nursing care Medical University, China and enhance their sense of responsibility. Results: The treatment of patients was not broken off, and no Purpose: Inquiry the clinical value that the hyperbaric complication happened. Conclusion: It is key factor for oxygen (HBO) with drug in treating chronic appendicitis. patients with tracheotomy to keeping breathe a lucid Method: 116 chronic appendicitis patients is divided into before hyperbaric oxygen therapy, middle and after that. treating set and matched set random, the treating set treated with HBO and drug comprehensive, the matched set with applied drug only. Results: Treat set 58 cases, recovery 47 (81.0%), valid 9 (15.5%), invalid 2 (3.5%), K178 total efficiency 96.5% ；Matched set 58 cases, recovery 35 Observation of curative effect of hyperbaric oxygen therapy on 3 cases of severe trauma patients in (60.3%), valid 16 (27.6%), invalid 7 (12.1%), total wenchuan earthquake efficiency 87.9%.Two sets of total efficiency have shown Qi Yan the relatively difference (p<0.01). Conclusion: HBO with the Second Provencal People’s Hospital of Guangdong , drug comprehensive treatment in chronic appendicitis has Guangzhou, China the relatively effect, and has important clinical application value. Objective: TO observe the curative effect of hyperbaric

oxygen on 3 cases of severe trauma patients in

Wenchuan earthquake. Method: Besides conventional

therapy, such as support therapy, anti-inflammatory K176 treatment and anticoagulant therapy, the 3 cases are Interfering effect of hyperbaric oxygen on the patient treated by HBO per day; 15 days are 1 course of of cerebral tumor and cerebral artery tumor after treatment. To observe the curative effect of hyperbaric operation oxygen after 1 day, 3 days and 7 days, respectively. Cunge Ren, Fen Wei, Futing Ma Result: The symptoms of dizziness and headache of the The Affiliated Hospital of AirForce Aviation Medical 3 cases have disappeared, The quality of sleep and Research Institute of PLA, Beijing, China emotion have been improved, the hematoma has been

obviously absorbed, the wound has healed well, The Objective: To approach the interfering effect of sensory function has recovered gradually, the muscle hyperbaric oxygen on the patient of cerebral tumor and strength has been increased above 1 grade, which total cerebral artery tumor after operation. Methods: 30 effective rate is 100 percent. Conclusion: It is effective patients of cerebral tumor and cerebral artery tumor after and safe for the severe trauma patients in earthquake operation were divided equal 2 group by the method of early to obtain hyperbaric oxygen therapy on the basis of semi-stochastic, the hyperbaric oxygen exposure group conventional therapy. and the control group. The former group was cured by hyperbaric oxygen based on routine method, the later were only carried through injection of nerve-nutrition and sustain drug. Results: The therapy effect of 2 group K179 have obvious distinct in directional thought, daily behavior, The Influence of Anxiety on the Effect of Sudden improve of the remembrance, the ability of self-control Sensorineural Hearing Loss (P<0.05). Conclusion: The therapy of hyperbaric oxygen Jiang Yan, Wang Rongzhen, Chen Lijuan, Ding Xiaohong, are advantaged in nerve tissue rehab, eliminated the Lu Wei oppressed symptoms of nerve tissue, reduce the Dept. of HBO, First Affiliated Hospital of College of sequela etc. It can shorten the time window of miss the Medicine, Zhejiang University opportunity of nerve function obviously, and improve the life quality of patient. Objective: To study the influence of anxiety on the effect

of sudden sensorineural hearing loss. Methods: 80 cases

were divided into two groups according to anxiety scores.

Group 1 had 57 cases, anxiety scores was more than 40. K177 Group 2 had 23 cases, anxiety scores was less than or Hyperbaric Oxygen Therapy Nursing of Patients with equal to 40. All the cases were treated by sympathetic Tracheotomy method of medicines combined with hyperbaric Qian Chunling, Wang Neng oxygenation. Result: The general effective rate of the two Chizhou People’s Hospital, Anhui, China groups was 53% and 85% respectively, with significant

Objective: To analyze the medical safety and effect of difference (p<0.05) ．Conclusion ：：：Anxiety has significant hyperbaric oxygen (HBO) therapy in patients with influence on the curative effect of sudden sensorineural tracheotomy. Methods: The paper analyzed the situation hearing loss ． of the50 patients treated with hyperbaric oxygen, holding

water content, infarct volume, and cell death were evaluated. Enzymatic activity of capase-3 and -9, K180 expressions of cytochourome c, Bcl-2 and Bax proteins The clinic experience of Hyperbaric oxygen in were performed in the samples from hippocampus, Epilepsy Research Summary and Recommendations ischemic penumbra and core, respectively. Results : Jinxiang Xie 1, Yongzhong He 2 HBO-PC reduced brain edema, decreased infarction 1. Shantou Central Hospital Affiliated Shantou Hospital of volume, suppressed mortality, and improved neurological YAT-SEN University, China recovery. HBO-PC decreased caspase enzyme activities, 2. Department of pediatrics, Longhu Peoples Hospital, upregulated BCl 2 expression, and reduced cytoplasm Shantou, China cytochourome C levels. Conclusions : HBO-PC protects brain tissues from ischemic reperfusion injury by Aims: The purpose of this study is to sum up the clinic suppressing mitochondrial apoptotic pathways. experience of Hyperbaric oxygen (HBO) in Epilepsy (EP) and make some sound recommendations. Methods: Several aspects, including means and main points of therapy, use of anti- epilepsy drugs (AEDs) and K182 electroencephalograph (EEG), evaluation and affected Clinical observation of hyperbaric oxygen and drug factors of curative effect , of Clinic data about HBO in EP treatment on facial neuritis ware analyzed and concluded. Results: WEI Fen, MA Futing, REN Cunge Recommendations: ①Theory and practice research of Affiliated Hospital of Institute of Aviation Medicine of Air Force, Beijing, China HBO in EP should be enhanced. ②Better therapeutic Objective: To observe the clinic curative effect of process of HBO in EP should be explored persistently. ③ hyperbaric oxygen and drug treatment on facial neuritis. EP can be considered as one of the diseases treated by Methods: The subjects included 45 patients, which were HBO. Sound curative effect of HBO in EP can be obtained randomly divided into two groups: treatment group (n =21, according to choosing subjects correctly, seizing the 1 drug therapy plus hyperbaric oxygen, 0.2MPa curative time, working properly, using EEG and AEDs. 99.5%oxygen, oxygen inhalation60min, 10days as one Conclusion: Hyperbaric oxygenation therapy (HBOT) in course) and control group (n =24, drug therapy: EP has its own merits, such as short period of treatment, 2 prednisone 20-30mg/d po, and given vitB1 100mg+vitB12 sound curative effect, no obvious side effect. Therefore, 500ug, take intramusular injection for 14days). Results: HBOT is a new and secure kind of technique and it can be Comparing the changes of clinical symptoms after one used extensively. Affirmatively curative effect of HBO in course of treatment, there were notable difference in the most clients with EP can be achieved and its effect mainly curative rate (91.7% vs 84.2%) between treatment group lies on the results of the primary diseases. and control group. The therapeutic effect of the treated

group is better than that of the control group (P<0.05).

Conclusions: Early HBO and drug therapy can shorten

the course and raise the cure rate of facial neuritis. K181

Hyperbaric oxygen preconditioning reduces ischemic-reperfusion injury by inhibition of apoptosis in a middle cerebral artery occlusion rat model K183 Jiasi Li 1, Zhi-min Kang 2 , Su-ju Ding 1, Wen-wu Liu 1, 3 1 The discussion on static-preventate -discharge John Zhang , Xue-jun Sun technologyfor single human hyperbaric oxygen 1. Department of Diving Medicine, Faculty of Naval chamber Medicine, Second Military Medical University, Shanghai, Jianhua Yang, Yan He, Caijun Mei China Affiliated Hospital to Chuanbei Medical University, 2. Changhai Hospital, Second Military Medical University, Sichuan, China Shanghai, China

3. Department of Physiology and Pharmacology, Loma Objective: This paper analyses and explains the Linda University School of Medicine, Loma Linda, objective properties on HBOC static electricity existence California, USA so that inspire the manufacturer of single human hyperbaric oxygen chamber pay more attention to possible static harm in design, manufacture and install. Objective : This study examined the mechanisms of Methods: We discuss the static-preventate -discharge hyperbaric oxygen preconditioning (HBO-PC) on ischemic technology about the manned HBOC vessel with the aids reperfusion brain injury. Methods : Male Sprague-Dawley of some directed or indirected static electricity rats (250-280g, n=144) were divided into control, middle experiments and some actual circumstances of the cerebral artery occlusion (MCAO) for 90 min, and MCAO three-variety single human hyperbaric oxygen chamber in plus HBO-PC groups. HBO-PC was conducted four times our hospital . Results: The static-preventate -discharge by given 100% oxygen at 2.5 atmosphere absolute (ATA) , technology for the human-body or the equipment units in for 1 hour at every 12 hour interval for 2 days. At 24 hour the HBOC have feasibility and are practical. Conclusion: after the last HBO-PC, MCAO was performed and at 24 The static-preventate -discharge technology for the hour after MCAO, mortality, neurological function, brain human-body or the equipment units in the HBOC need to be done some researches and improved deeply so that Monitoring effect of disinfection in hyperbaric oxygen the safties are in the course of running the single human chamber and oxygen humidified tank and measures HBOC at any time. of nosocomial infection control Lei Xue, Xiaohui Liu Department of hyperbaric 0xygenation, Nan fang hospital, Southern medical university, Guang-zhou, China K184 Risk Management and Preventive Measures of Objective: To disinfect the air of hyperbaric oxygenation Hyperbaric Oxygen Chamber (HBO) chamber, article surface of HBO chamber and YAN Xiaomin oxygen humidified tank and monitor their bacteria The First Affiliated Hospital, Chongqing Medical University, counting and identification regularly. To improve the way Chongqing, China of disinfection with monitoring effect and control nosocomial infection. Methods: The ways of disinfection Objective: To discuss risk management and preventive included cleaning up HBO chamber and ventilating by measures of hyperbaric oxygen chamber. Methods: To electric fan, cleaning article surface of HBO chamber with identify and evaluate the risk factors of hyperbaric oxygen disinfectant and water, disinfecting HBO chamber with therapy. Results: preventive measures for risk disinfector for 30min~60min. Before HBO therapy and management of hyperbaric oxygen chamber were after disinfecting of HBO therapy, the air of HBO chamber, constituted. Conclusions: To establish the holistic article surface of HBO chamber and oxygen humidified operation regulations and implementation of risk fluid of oxygen humidified tank were sampled regularly. management of hyperbaric oxygen chamber are ultimate The samples were analyzed with bacteria counting and guarantee for patient safety. identification. The effect of disinfection was be used to improve the way of disinfection control nosocomial infection. Results: Our Disinfecting of HBO Therapy kept the bacteria counting in the air of HBO chamber at K185 ≤500cfu/m 3 and on article surface of HBO chamber at 3 Study on brainstem reflex in patients with primary ≤10cfu/m , fulfilled the requirement of class Ⅲ brain stem injury treated by hyperbaric oxygenation environmental sanitation standard. The bacteria counting integrated therapy in oxygen humidified fluid of oxygen humidified tank kept Lei Xue, Gangchuan Wu 3 at < 10 cfu/ml. Conclusions: Our Disinfecting Measures Department of Hyperbaric Oxygenation, Nan Fang of HBO therapy can controls nosocomial infection. Hospital, The Southern Medical University, Guang-zhou,

China

Objective: To investigate the predictive values of K187 brainstem reflex (BSR) on the evaluation of severity, the The effect of combined hyperbaric oxygen on severe effect and the prognosis in the patients with primary brain head injury (report of 42 cases) stem injury treated by hyperbaric oxygenation (HBO) Zhihui Xie, Jianguo Li, Zuoxun Chen integrated therapy. Methods: Sixty patients were divided The Affiliated Hospital of Zunyi Medical College, Guizhou, into two groups: HBO group (n=30) and control group China (n=30). HBO group were treated with HBO integrated therapy. Control group were treated without HBO Objective: To investigate the clinical effect of hyperbaric conventional integrated treatment. During the treatment oxygen (HBO) therapy on severe head injury . Method: 42 BSR were monitored at once every day for finishing cases with severe head injury received combined HBO treatments. The relationship for BSR ,Glasgow coma and the effect was determined according to the clinical scale (GCS) and Glasgow outcome scale (GOS) were curing criteria of diseases diagnosis. Results: Recovery analyzed. Results: The BSR were closely related with in 7 cases (16.7%),improvement in 30 cases (71.4%) and GCS and GOS. The lower of disappear-level of BSR, inefficacy in 5 cases (11.9%),the total effective rate was GCS and GOS, the worse of the prognosis of the patients. 88.1%.The effect was better in the group of short course The death have 3 scores in GCS, BSR disappears on the than the group of long course, and it was significant level of number Ⅳ～Ⅵ. The BSR were to be an index especially within twenty days(the total effective rate was used for adjusting the proposals of HBO treatment. 93.3 ～94.4%). The total effective rate of the children Conclusions: BSR were considered to be valuable to the below ten years old was higher obviously than that of the evaluation of severity, the effect and the prognosis in the patients with primary brain stem injury treated by HBO adult (it was 100%,84.3% ～ 85.7% respectively). integrated therapy and to be an index used for adjusting Conclusions: As an effective treatment, HBO plays a the proposals of HBO treatment. specific role in remedy of the acute and severe head injury. HBO should be applied to the patients with severe head injury as early as possible if their conditions allow. Moreover, HBO has significant effect on severe head K186 injury in children compared with the adult; therefore, it should be adopted early.

symptoms of headache, dizziness, diplopia or vomit in the pressure chamber. the doctor should stop compress, K188 respire oxygen and reduce pressure slowly. Then An Investigation on hyperbaric oxygen in premature reexamine head CT, to prevent the gas from entering infants with hypoxic-ischemic encephalopathy and encephalocoele and cause pneumatocephalus. the long-term effect Yongzhong He 1, Jinxiang Xie 2 1. Department of pediatrics, Longhu Peoples Hospital, Shantou, China K190 2. Shantou Central Hospital, Affiliated Shantou Hospital of The first to enter is last to exit law of gas saturation YAT-SEN University, China and de-saturation Xiao Pingtian Aims: According to reviewing and summing up the Xiangya Hospital of Centre South University, Changsha, short-term and long-term effect of Hyperbaric Oxygen China Therapy in premature and underweight Infants with Hypoxic-ischemic Encephalopathy (HIE), as well as the objective: To investigate the law of gas getting into or out experience, the purpose of this study is to recommend of the body during changes of environmental gas pressure. some principles of Hyperbaric Oxygen Therapy. Method: We used two methods of cabin operation to Methods: Based on analyzing the clinic data and the compare the region of variation of real efficiency figures of primary therapy, infant hyperbaric oxygen chamber was partial oxygen pressure (PO 2T) and partial nitrogen used, the pressure was 0.13MPa, the content of oxygen is pressure (PN 2T) in five kinds of hypothetical tissues, and between 72% and 75%, and once a day, 60min at a time, with different compression time and different steady 9.6 times for every infant on average. Effect of just pressure: In procedure I, patients were assigned to inspire terminating therapy and seven years old was assessed. air during the compression stage; In procedure II, the Results : All 33 infants recovered and leaved hospital patients inspired oxygen during the compression stage. after Hyperbaric Oxygen Therapy. However, during the Results : ①The PO 2T of procedure II was markedly larger observation, one died and five with serious HIE infants than that of procedure I and the PN T of procedure II was had sequelas, the rest with serious HIE and all with slight 2 or moderate HIE recovered completely, the total efficiency markedly less than that of procedure I ；②The size of is 96.7 % (32/33). One infant in 32 has cerebral atrophy in region of variation of PO 2T and PN 2T in both procedures CT for brain and the other are normal. Side-effect such as was in direct proportion to the speed of gas saturation of Broncho pulmonary dysplasia (BPD) and Retinopathy of the tissues ；③The PO 2T and PN 2T of all groups increased prematurity (ROP) has not been discovered. Conclusion: as the steady pressure increased. The PN Tof procedure According to the research of BPD and ROP made in 2 I of all group increased on lengthening the time of abroad, in principle, Hyperbaric Oxygen Therapy should compression, but the PO T was not increased, and, on be forbidden in premature and underweight infants. 2 the contrary, it reduced a little. Conclusion: The law of

gas going into or out of the body is that the gas that is K189 saturated first, is the last to de-saturate (first to enter is last to exit), and this law is mainly to increase the time A case of hyperbaric oxygen treatment causing efficiency of hyperbaric effect. The law of “first rapid, later pneumatocephalus slow” is to raise the pressure efficiency of gas saturated in Rong Xie, ZhiQiang Zhuang advance of hyperbaric effect. These two laws are the PuNan Hospital, Shanghai, China significant theoretical basis for hyperbaric oxygenation

pretreatment and the reason that procedure II is selected Objective: To approach the pneumatocephalusability to replace procedure I. However, when procedure II is when hyperbaric oxygen treatment for acute brain injury patient. Method: reporting a practicality. Result: a patient applied, the compression time should not be overly prolonged. suffered a acute brain injury and got left frontal bone fracture. We cured him with hyperbaric oxygen. The pressure of chamber is 0.12Mpa, the oxygen uptake time is 30 minutes, then interrupt with 5 minutes air, and input K191 oxygen for another 30 minutes, the compression time 15 The effect of hyperbaric oxygen on consciousness minutes, and lower to atmospheric pressure with 20 recovery of the coma patients suffering from brain minutes. After 5 times of hyperbaric oxygen treatment, ischemia re-perfusion head CT of the patient showed elliptical gas density in left front brain. Conclusion: On the occasions of the head Xiao Pingtian Xiangya Hospital of Centre South University, Changsha, lacerated wound, the fractured skull or skull bottom, China around the eye socket hypodermic and the conjunctiva ecchymosis, the external auditory meatus or nasal passage had the bloodstain, cerebrospinal fluid leaks Objective: To investigate the effect of hyperbaric oxygen from nose, ear or eye. malfunction of smell, the vision to on consciousness recovery of the coma patients who be fuzzy, and symptoms of oculomotorius damage. It is suffer from brain ischemia re-perfusion. Method: 11 necessary to take a head CT before making the hyperbaric oxygen treatment. If the patient shows some cases of coma patients suffering from brain ischemia re-perfusion were recruited in this study. The clinical data were of grade 0~I. The Babinski’s signs of the lower limbs of the treatment of hyperbaric oxygen were collected and were negative. The cerebrospinal test indicated the analyzed. Results : 1) all five subjects with full recovery corruption of blood brain barrier (BBB), the dispropotion received hyperbaric oxygen treatment within 60 hours increased of IgG. And the MRI of the spine revealed: after coma; 2) There were two cases with little intelligence abnormal signal of spinal cord (T4~T11), advisement damage. One patient were treated with hyperbaric oxygen diagnosis was acute myelitis. Then the patient was given five 5 hours after coma. The other one received high-dose hormone implusion treatment, associated with sub-hypothermia treatment immediately after the neurotrophic drugs. Three days later, the patient could occurrence of cardiac arrest, and started hyperbaric urinate by herself. On the ninth day, the patient was noted oxygen therapy at 21st day. 3) One subject receiving to have pathologic signs of neurosystem and can defecate hyperbaric oxygen three days after brain ischemia coma herself. But her lower limbs were still disability. Ten days was in persistent vegetative state. Another patient starting after the patient entered the hospital, she took the oxygen hyperbaric oxygen four days after coma died finally. treatment. One oxygen chamber session finished, the Conclusion: The prognosis of the coma patients suffering patient’s muscle force of lower limbs increased to grade from brain ischemia re-perfusion is related to many factors. Ⅲ－Ⅳ and the muscle strain also improved. PTR test An appropriate treatment opportunity is critical to the exist. She can walk by help. When two oxygen chamber protective effects of hyperbaric oxygen on brain cells in sessions finished, the patient recovered, she can walk by the coma patients suffering from brain ischemia herself. The PTR test performed showed normal exist, the re-perfusion. muscle force was grade Ⅳ—Ⅴ. This case the patient had unsatisfactory effect under normal treatment, however, combined with hyperbaric-oxygen treatment, the paralysis K192 gradually reduced, muscle force improved, and the patient Safe oxygen inhaling device without resistance in could walk without help. The hyperbaric-oxygen treatment hyperbaric oxygen could improve the spinal oxygen saturation and the Wu Zhide pressure of spinal oxygen, increase the oxygen dispersion, Xiangya Hospital, Zhongnan University, Changsha, China thus could improve the anoxic situation of spinal cord, decrease the dropsy of spinal cord, and also improve the Objective: To evaluate the clinical value of a new oxygen function of BBB, alleviate the inflammational inhaling device by observing the oxygen inhaling conglutination of the tissue. The hyperbaric-oxygen resistance and oxygen concentration in chamber. Method: treatment could also extend the time of neurocell To divide into three groups: pulmonary oxygen supply regeneration; protect the reversible nerve, which in the apparatus (A group), continuous oxygen supply apparatus end improve the function of the nerve. Since the BBB (B group), and new oxygen inhaling apparatus (C group). dysfunction could easily result in paralysis, we Oxygen inhaling resistance and oxygen concentration in recommended the use of hyperbaric-oxygen treatment chamber of three groups were measured. Result: there and especially use this remedy at the acute period of the was significant difference for oxygen concentration in disease. chamber in three groups (P<0.05), B group was obvious higher than A group and C group(P ＜ 0.01), But no significant difference was found between group A and K194 group C(P ＞0.05); A group was obvious higher than B The safe nurse of hyperbaric oxygen treatment on group and C group in oxygen inhaling resistance(P ＜0.01), moderate to severe craniocerebral injury patient Wu Qiaoning But no significant difference was found between group B The Six Affiliated Hospital of GuangXi Medical University and group C(P ＞0.05). Conclusion: New oxygen inhaling device can diminish the oxygen inhaling resistance and Objective: To explore the preferred way of the safe nurse cut down oxygen concentration in chamber, in favor of the of hyperbaric oxygen treatment on moderate to severe treatment and safety of hyperbaric oxygen, then to be craniocerebral injury patient. Methods: According to worth further clinically generalization. clinical classification, pars affecta, associated injury, operation, pathogenetic condition, complication and so on,122 moderate to severe craniocerebral injury patients were adopted correspondence safe nursing intervention K193 at prior hyperbaric oxygen treatment, during hyperbaric Hyperbaric oxygen for acute myelitis----one case oxygen treatment and post hyperbaric oxygen treatment. report Result: Because of safe nursing intervention is targeting, Haibin Xiang, Rong Xie all of the 122 moderate to severe craniocerebral injury Shanghai Punan Hospital, Shanghai, China patients can complete hyperbaric oxygen treatment safety and successfully, and there are no the complication and A 8-year-old girl entered the hospital with the chief accident of hyperbaric oxygen treatment. The curative complaint “Lower limbs fatigue, following walking effect is satisfaction. Conclusion: Reinforcement on prior, dysfunction and the obstacles of urination and during and post hyperbaric oxygen treatment safe nursing defecation ”. PE: Disapear of PTR and ATR tests. Muscle is the key point of treatment. strain declined, and the muscle force of the lower limbs of transfusion was not clear. His symptoms were controlled for a time. However, the headache and K195 dizziness increased, and he did not have a response to Prevention and Treatment on the decompression treatment with transfusion, the patient was scheduled for sickness of diving work fishermen oxygen treatment on Dec27th 2007. The protocol of Wen Chengwen hyperbaric-oxygen treatment was: exposed to 100 People’s Hospital, Hainan, China percent oxygen at 1 and then 2.2 atmospheres absolute during the chamber session. After five times oxygen Objective: To study prevention and cure method and chamber treatment, the patient recovered and remained treatment effect of the decompression sickness diving chest bored and limb fatigue. When one oxygen chamber work fisherman. Method: The object of treatment is session finished, the patient was evaluated in the divided into patient’s team and health cure treatment set. out-patient clinic. Additional information were obtained, it It is adopted dissimilar treatment project of the Chinese air was learned that the chest bored disappeared, the diving decompression sickness compression treatment headach and dizziness decreased, and the limb form. Result: The treatment efficient of patient team is numbness also decreased. After two oxygen chamber ninety porcent. The decompression sickness rate of sessions, the symptoms of the patient dissapeared. health cure treatment set is 0.67%. Conclusion: For the Discussion: Methyl chloroform was a kind of low toxity fisherman of diving work, It should carry on compression poison. Reports on its poisoning mechanism on health treatment periodical. For the patient of decompression were poor. The approach for its poisoing was sickness, It should carry on compression treatment as predominately through aspiratory system. The target early as possible. apparatus of methy chloroform was neural system. When exposure in low-concentration methyl chloroform, the symptoms were restrain effects of neural system such as body im-balanced. However, the exposure in K196 high-concentration methyl chloroform would lead to an Clinical observation of hyperbaric oxygen and aesthesia-like symptoms of the neural system, even the ginaton in the treatment of sudden deafness loss of respiratory and resulting in the death of patient. In Cuixia Xue, Xinli Zhang, Lin Wang this case, the patient was inadvertently inhaled the methyl Department of hyperbaric oxygen, The First People’s chloroform gas during work, which resulting in the nerual Hospital of Qingdao Economic and Technological system symptoms such as headache and dizziness, Development Area, Shandong, China unsteady steps, limb fatigue, vomiting, chest boring and weeping. All the clinical representation was consistent Objective: To investigate the effect of hyperbaric oxygen with the data. The report of hyperbaric-oxygen treatment (HBO) combined with ginaton for sudden deafness (SD). on the methyl chloroform poisoning was very poor. This Methods : 109 patients with sudden deafness were case was gained satisfied effect by using oxygen chamber divided into hyperbaric oxygen group and control group at treatment. The mechanism of it was need further random. Both of the two groups were given ginaton. 57 investigated. patients with SD were exposed to HBO, which also were divided into group A that was exposed to HBO in 3 days after getting SD and b that after 3 days. 52 patients for comparison (non-HBO group) were treated only by K198 ginaton without HBO. Results: The overall effective rate The Effects of the Hyperbaric Oxygen on in HBO group (84.2%) was higher than that in non-HBO Consciousness Recover With Serious Brain Injury group (65.4%) (χ2=4.211, P<0.05). Furthermore, it in Wu Qiong 1, Weng Qibiao 2, Wang Hongjun 2, Chen group A (96.3%) was higher than that in group B (73.3%) Lingzhen 2, Chen Xuelin 2, ChenQiuYan 3 (χ2=4.041, P<0.05). Conclusion: Therapeutic effect of 1. Taishan People’s Hospital of Jiangmen City, HBO combined with ginaton for SD is significantly better Guangdong Province, China than that of simple ginaton without HBO. 2. Department of Hyperbaric oxygen, Zhujiang Hospital attached to Southern Medical University, China 3. Nanhai People’s Hospital of Foshan City, Guangdong K197 Province, China Case Study: A 56-year-old Man with acute methyl chloroform saturation Aim: The effects of the Hyperbaric Oxygen on Shi Chunmei consciousness recover with serious brain injury. Method: Shanghai Shuguang Hospital, Shanghai University of Using single hyperbaric oxygen chamber to cure the Traditional Chinese Medcine and Pharmacology, patients whose brains are seriously damaged before Shanghai losing their consciousness in 41 cases. Scheme: 0.2MPa 20min ×2+40min.using the Hyperbaric oxygen chamber to Clinical data: A 56-year-old man was inadvertently cure the patients after their consciousness exposure in methyl chloroform gas on Dec21st 2007, immediately the patient felt headache and dizziness, recovered.Scheme:0.20Mpa 20min ×2+90min. Result: 29 unsteady step like drunk, limb fatigue and numbness, in 41 cases recoveredcure rate: 70.73%. Conclusion: malaise and vomiting. The patient was seen in the The Hyperbaric Oxygen is helpful in curing patients whose out-patient and given transfusion treatment. The tremedy brains are seriously damaged and it can reduce the rate of Zhujiang Hospital, South Medical University, China persistent vegetated situation. Objective: Value the Therapeutic effect hyperbaric oxygen treatment for Craniocerebral Injury. Methods: Totally 192 patients samples were enrolled, all the patient K199 s are randomly assigned to three groups. Group A (control The prognosis analysis of different hyperbaric conventional therapy group). Group B (giving hyperbaric oxygen treatment period after cerebral hematoma oxygen treatment after Craniocerebral injury for 20 to 30 removal days) and gaining satisfied effect. Results: Stress Wen Li, Jinlong Sha, Haili Tang, Yan Yang, Hua Qiu ulcerbleeding occur rate: There was significant difference Dongguan Hospital, Dongguan, China between group B and group A (P < 0.05) after Craniocerebral Injury for 20 to 30 days. And no significant Objective: To investigate the effects of hyperbaric difference between other groups ( P < 0.05) on rest stage oxygen treatment during the different period after and groups. Conclusion: Hyperbaric oxygen treatment intracerebral hemorrahage removal. Methods: 73 shows obvious curative effect for Craniocerebral Injury, postoperative patients after intracerebral hemorrahage and treatment after injury for 20 to 30 days is the best removal were divided into early treatment group (group A, performance time. HBO is an important and active within 14days after operation, the patient number is 38, measure in curing the Craniocerebral Injury. Male:25, Female:13,age:28~70Y,average age:58Y) and later treatment group (group B, more than 14days after operation, the patient number is 35, Male:26, Female: 9, age:30~69Y, average age:61Y) according to the K202 beginning time of therapy. Results : group A ’s efficient Investigate the effect observation and coordinate with rate is 94.73%, group B ’s efficient rate is 71.43%. To humanistic care nursing strategy of high-pressure compare the early treatment group with its counterpart, oxygen (HBO) treatment of persistent vegetative state there were significant difference (P < 0. 05) on the (PVS) improvement of therapeutic effect and daily living activity Wu Ruilan between two groups. Conclusion: The therapeutic effect HBO Center, Affiliated Hospital of Hainan Medical College, of early treatment group is better than that of later Hainan, China treatment group, The more early to start hyperbaric oxygen treatment, the better prognosis we can get. Objective: Investigate the effect observation and nursing strategy of high-pressure oxygen (HBO) treatment of persistent vegetative state (PVS) patients. Methods: 134 cases of PVS patients were randomly divided into two K200 groups: HBO treatment (n = 84) and conventional Prevention and treatment of carbon monoxide treatment group (n = 50), testing PVS before and after the intoxication and tardus encephalopathy by HBO treatment, and contrast with HBO after treatment efficacy Xia Huang, Guoyi Ren, Wenhui Li and duration, frequency, causes relations, coordinate with Zhujiang Hospital, South Medical University, China humanistic care, observation of treatment. Results: The total efficiency of HBO group is 85% and conventional Objective : To investigate the therapeutic effect of treatment group is 52%, respectively X2 = 16.59. P<0.01, hyperbaric oxygen (HBO) on acute carbon monoxide the effect of HBO treatment was significantly better than intoxication and the feasibility on prevention of tardus conventional treatment group ；compared to conventional encephalopathy. Methods : The clinical data of 46 cases treatment before and after, the statistical difference was of moderate or severe acute carbon monoxide intoxication no significant (P>0.05). Conclusion: HBO treatment to treated with hyperbaric oxygen (HBO group) and 30 cases PVS patients with a positive role in restoration, and have of acute carbon monoxide intoxication with regular closely related with the duration of treatment, frequency treatment except HBO (control group) were analyzed. and causes. The appropriate care during treatment, will Results : The effective rates were 97.83% for HBO group reduce the pressure of patients, and establish confidence and 76.67% for control group. There was a significant in the treatment and improve treatment. difference between the two groups ( p<0.05). The incidences were 2.94% for HBO group and 13.33% for control group. There was a significant difference between the two groups ( p<0.05). Conclusion : Hyperbaric oxygen K203 treatment can reach an obvious therapeutic effect on The effect of hyperbaric oxygenation therapy on the acute carbon monoxide intoxication and reduce the flash electroretinogram in rats with experimentally incidence of tardus encephalopathy. elevated intraocular pressure

Wu Shuanggui

Lengshuijiang People’s Hospital, Hunan, China

K201 Objective: To investigate whether hyperbaric Clinical therapeutic effect of hyperbaric oxygen oxygenation therapy can improve the electroretinogram in treatment for craniocerebral injury rats with experimentally elevated intraocular pressure Quyun Yang, Qibiao Weng (IOP). Methods: Acute elevated IOP model was built in old and is divided into more than 50-years old with the each eye of 18 Wistar rats, then they were divided into A effect of observation. 2Checks can be divided into mild, and B groups randomly. Group A (no HBO group), nine moderate and severe when the effect of HBO. 3After rats were not used any therapy. Group B (HBO group), treatment from the disease sooner or late can be divided nine rats used hyperbaric oxygenation therapy after into less than a week and less than tree months, more model building. The electroretinogram was recorded than three months to six months when the effect of six again after 7 days. Results: The b wave recoveries of the months. Sudden deafness hyperbaric oxygen treatment electroretinogram in group A were (41.85±13.20)%; that in efficacy significantly. Results: According to the incidence group B were (60.04±13.20)%. The difference between of different ages and the length and pure-tone audiometry, two groups was statistically significant (P<0.01). the clinical course of the 3 - 4 separate high-pressure Conclusions: Hyperbaric oxygenation therapy promoted oxygen, treatment, younger, the higher the cure rate and the FERG b wave recoveries. It indicated that HBO efficiency, the shorter the onset time, the cure rate and therapy could protect retina on fighting against the more efficient, pure-tone audiometry, classification, the ischemia injures. more light deafness, the higher the cure rate and efficient. Conclusion: HBO treat of sudden deafness the clinical efficacy and age, the incidence of time and the severity of the illness. Middle-aged incidence after treatment sooner, K204 the best effect. Hyperbaric oxygen and traumatic brain injury Yali Wu, Lianbi Xue Hyperbaric Center of Beijing Tiantan Hospital, Capital Medical University, Beijing, China K206 Analysis of the therapeutic effect of low molecular weight heparin sodium combined with hyperbaric Objective ：：：Traumatic brain injury (TBI) is called the silent oxygen in patients with acute cerebral infarction. epidemic in china, because of which our society is now Lianhua Wu, Chunjin Gao, Huan Ge, Guozhong Wang, suffering from heavy economic burden. All kinds of Liming Zhao, Yu Gao, Yi Zhang, Linlin Ma, Fujia Liu medicine and hypothermia treatments have failed to Department of Hyperbaric Oxygen, Chaoyang hospital improve the TBI patients’ functional outcome and lower Affiliated of Capital University of Medical Sciences Beijing, down mortality rates. Recently, oxygen therapy has China shown a great effect in the animal and clinical research, and it is quite promising to treat TBI with hyperbaric Objective: To investigate the therapeutic effect of low oxygen (HBO) therapy. So the HBO therapy is getting molecular weight heparin (LMWH) combined with more and more important in the treatment of the acute TBI hyperbaric oxygen (HBO) on Patients suffered from acute and in the process of recovery. Consequently, this review cerebral infarction. Method: 203 patients with acute brain summarizes the current mechanisms of HBO treatment in infarcted were divided in to 2 groups firstly. 101patents in traumatic brain injury (TBI) and their efficacy. Methods: Directed literature review. Results: It was considered that control group, were treated by0.1ml/10kg LMWH Sodium ， the cerebral vasoconstriction and increasing the oxygen hypodermic injection, once per 12 hours, treatment begun utilization were the primary mechanisms of HBO in in 72 hours after acute brain infarcted. for a treatment treating TBI. But now, it is found that HBO can improve course of 7 days, There were102patents in the HBO the cellular level cerebral aerobic metabolism so as to group, were treated with LMWH Sodium combined with make the damaged mitochondrial recover. Furthermore, HBO witch was once a day for 20days.Neurological HBO, with the ideal treatment paradigm of 1.5 ATA during impairment scores (NIS) and ability of daily life (ADL) 60 minutes, does not produce oxygen toxicity so the scores (Barthel index)were used as effective parameters patients will be relatively safe. However, although whether before and after treatment in two groups . Results: The to use HBO to treat TBI, remains controversial, more and NIS was significant lower in HBO group than in control more evidences show that HBO may be a method to treat group (p<0.01)and ADL score of the HBO group was the patients with brain injury. So further investigations are higher than control group(p<0.05) after treatments. required to prove the effect of HBO in treating TBI and the Conclusion: Low Molecular Weight Heparin combined pharmacy and treatment opportunity still need research. with Hyperbaric Oxygen has more therapeutic effect than Low Molecular Weight Heparin in treating patients of acute cerebral infarction.

K205 Hyperbaric oxygen treatment and nursing of 33 cases of sudden deafness K207 Wu Yanqiu Clinical study on the relate markers of blood Henan Provincial peoples Hospital in Zhumadian City coagulation in the patients with avascular necrosis of Centre, China the femoral head after severe acute respiratory syndrome Objective: Discussion HBO on the effect of sudden lianhua wu chunjin gao guozhong wang lin yang deafness observation and influence. Method: the 33 xiaomin hou huan ge chengqing xia man qi cases 1Outstanding deafness by age less than 50 fears Department of Hyperbic Oxygen ,Chaoyang hospital Affiliated of Capital University of Medical Sciences Beijing Hyperbaric Oxygen Department, Haikou Municipal China People’s Hospital, China

Objective: To investigate the blood coagulation function ISO refers to International Organization for in the patients with avascular necrosis of the femoral head Standardization. The essence of standardization is the after severe acute respiratory syndrome. Method: we design and implementation of all kinds of rules and measured respectively the expression of CD 31, CD61, regulations. The design and implementation of rules are CD 62p, CD63 and PAC-1 on platelet membrane by absolutely necessary for treatment with hyperbaric flowcytometry, and measured the plasma prothrombin oxygen chamber. We can assure absolute safety of time (PT), activated partial thromboplastin time (APTT), hyperbaric oxygen chamber in treatment process, thrombin time (TT) ,and fibrinogen (Fbg) by blood clotting increase the overall quality of the Hyperbaric Oxygen instrument in 26 patients with avascular necrosis of the Department, increase the safety management level for femoral head after severe acute respiratory syndrome and hyperbaric oxygen chamber, improve all of the rules and in 17 healthy adults. Results: The expression of CD31, regulations in the process of modification and assure that CD61, CD 62p, CD63 and PAC-1 on platelet membrane in the safety management of hyperbaric oxygen chamber 26 patients were all lower than those in 17 healthy adults keeps at the best level only when the designed rules and (p0.05) in those markers between patients and 17 healthy regulations are strictly obeyed and firmly implemented to adults. Conclusion: The blood may not be in turn visible rules into invisible actions. ISO standardized hypercoagulable state in patients with avascular necrosis management is the top priority in the treatment process of the femoral head after SARS. with hyperbaric oxygen chamber as it is directly related with the life safeties of patients. Absolute life safeties of patients who are treated with treated with hyperbaric oxygen chamber can be assured only when all hidden K208 dangers are eliminated. The key to the safety The role of hyperbaric oxygenation on the management of hyperbaric oxygen chamber lies on the convalescence of brain injury at high altitude implementation as well as continued modification and Hongzhi Xia, Suzhi Li, Chengliang Huang, Xuehong Yang, perfection of all sorts of rules and regulations. Qing Zhang, Maoxu Wang Standardized management is extremely important in the Department of hyperbaric oxygenation,Military general safety management of hyperbaric oxygen chamber. hospital of Tibet, Lhasa, China

Aim: To evaluate the role of hyperbaric oxygenation (HBO) on the convalescence of brain injury at high K210 altitude. Method: 270 patients convalescence of brain Effects of hyperbaric oxygen on the content of injury treated with HBO adding to medicines compared inflammation mediators in the rat brain tissue with 98 patients treated with medicines at the same period. following fluid-percussive brain injury Result: The cure rate in HBO group was obviously higher Yue Li- na 1, Gao Chun-jin 2, Zhou Li-chun 1, Pang Bao-sen 2, than that in medicine group. Conclusion: HBO can Zhao Qi-hang 2, Wang Guo-zhong 2 ameliorative brain tissue anoxia quickly, increase contain 1.Department Of Neurology, Beijing Chaoyang of oxygen, increase the oxygen save, make blood vessel Hospital,Capital University Of Medical Science, Beijing, constringency of each organism, increase or reduce little China of sacral vertebrae iliac artery hem the discharge, 2.Department of Hyperbaric Medicine, Beijing Chaoyang ameliorative reticular formation function of brain stem, Hospital,Capital University Of Medical Science, Beijing, cerebella winning, benefiting to patients consciousness China instauration with wide awake, and can accelerate the disease cooking stove the clearance, hematoma Objective: To observe the changes of content of absorption and make organize the inside gum quality the interleukin-1beta(IL-1β),tumor necrosis factor- alpha cell to divide to increase in value, produce the large (TNF-α),Intercellular adhesion molecule-1(ICAM-1) and quantity gum quality the fiber the tissue that repair the interleukin-10 (IL-10) before and after hyperbaric oxygen harm, and establish the vice circulation, urge the in the injuried brain tissue of Sprague-Dawley rats capillanisum regeneration quickly, promote the tapetum following severe lateral fluid-percussive brain injury and to tissue recovered. explore the mechanism of hyperbaric oxygen on traumatic brain injury. Methods: 72 male Sprague-Dawley rats were randomly distributed into 3 different groups: traumatic brain injury group (TBI group), hyperbaric oxygen group (HBO group) and sham-operation group (SO group).The severe traumatic brain injury models were established by the fluid percussion device. After 6 to 72 hours of survival, K209 rats were killed, and about 200 mg of brain tissue that Standardized Management Based on ISO 9000 contained the injury site, as well as tissue proximal to this System is the Fundamental Assurance for Safety region,was isolated for homogenizing.The concentrations Management of Hyperbaric Oxygen Chamber of IL-1β, TNF-α, ICAM-1and IL-10 in the homogenate of Sun Li brain tissue were measured at 6h, 24h, 48h and 72h, respectively, by using enzyme-linked immunosorbent assay (ELISA).Protein levels were determined by the method of Bradford. Results : were expressed as means±stand deviation.The SPSS 11.5 software was used for statistical analysis. The differences between groups were compared using a One-Way ANOVA,followed by an inter-groups analysis using the Student Newman Keuls Test. Differences were considered significant if the P value was <0.05. Results: 1.The concentration of IL-1β in homogenate in the HBO group at 6h was significantly higher than that of the SO group (p<0.05), but lower than that of the TBI group (p<0.05). The concentration of IL-1β in homogenate in the HBO group at 24h was significantly lower than that of the TBI group (p<0.05). 2. The concentration of TNF-α in homogenate in the HBO group at 6h was significantly lower than that of the TBI group (p<0.05). 3. The concentration of ICAM-1 in homogenate in the HBO group respectively at 6h, 24h, 48h and 72h was significantly higher than that of the SO group (p<0.05),but lower than that of the TBI group (p<0.05). 4.The concentration of IL-10 in homogenate in the HBO group respectively at 6h, 24h and 48h was significantly higher than that of the SO group (p<0.05),but there was no significant difference compared with TBI group (p>0.05). Conclusions: 1. Fluid percussion device can establish a severe traumatic brain injury model of rat successfully. 2. IL-1β, TNF-α, ICAM-and 1IL-10, which took part in the inflammatory mechanism after trauma, were elevated in the injuried brain tissue following TBI. 3. HBO treatment can effectively relieve the inflammatory following TBI through inhibiting the expression of IL-1β, TNF-α and ICAM-1, but can not throuth elevating the expression of IL-10. HBO may play a role in preventing the secondry brain injury and protecting the injury brain through the above mechanisms. First Author’s Index

A Christtian, Heiden OR01 Renu, Agnihotri OR08 Xiaomin, Hou K47 Chavez, Alberto OR10 Te-Chun, Hsia P38 Cheng-Pu, Hsieh P42 B Guiping, Hu K79 Jiaxiang, Bai K11 Huijun, Hu K81 Dirk J., Bakker B1 Shengli, Hu OR28 Jian, Bao K64 Lingling, Huang P18 John M, Batle OR11 P52 Min, Huang K74 Michael, Bennett OR06 Xia, Huang K200 Zhumei, Bi K56 Xiang, Huang OR13 Jing, Bian K76 J C Mingyi, Ji K90 Jianmei, Cai K170 Yunping, Ji K89 Jorge, Calderón OR12 Jianping, Jiang K88 Hao, Chen K62 Wenbo, Jin K86 Jianhui, Chen K123 Lijuan, Chen K61 K Sailian, Chen K60 Mahito, Kawashima PL1 Yanhua, Chen P53 Lei, Kong K84 Yifei, Chen P15 Jacek, Kot OR05 Jincheng, Cheng OR21 Aihua, Kuang K83 Philippe, Colombani P25 L Frans, Cronjé PL2 Zhaopan, Lai K30 K34 Cao, Cui K73 Hui-Chieh, Lee P47 Yan, Cui K125 Changchun, Li K82 Chu, Li K58 D Chunfang, Li K130 Congying, Deng K32 Dongjuan, Li K69 Jordi, Desola PL11 Hongyan, Li K80 Yuqin, Du K59 Houcheng, Li K77 K78 K92 Shutian, Duan K46 Jiasi, Li P08 K181 Jinsheng, Li P28 F Li, Li K70 Wenqing, Fan K131 Lingfang, Li K124 Lianfeng, Fei P14 Mingxing, Li P51 John, Feldmeier OR02 P44 Peiyun, Li K132 Jochen, Freier PL5 Shouchao, Li P19 Shunyong, Li K24 G Wen, Li K150 K199 Chunjin, Gao P10 Xianghui, Li P21 Ningqin, Gao K57 Yajuan, Li K20 Xiudong, Gao K68 Youmin, Li K16 Yu, Gao P33 K55 Yuxian, Li K21 Chaoming, Ge K54 Zengmin, Li K15 K19 Huan, Ge K53 Zhang, Li K18 Guojun, Gu K66 Zhicai, Li K09 Guanghan, Guo K52 Zhuo, Li OR17 Junmei, Guo K51 Qinglin, Lian K165 Na, Guo K50 Shaohua, Liang K22 Stephen, Guthrie PL10 Xiuqing, Liang K14 Mao-Tsun, Lin OR07 H Shilong, Lin K12 Michal, Hajek OR03 Ying, Lin K13 Christer, Hammarlund OR14 Daodong, Liu K02 Cuihong, Han K167 Fang, Liu P34 Chunyang, He P20 Fujia, Liu K03 Guiying, He P13 Guangsen, Liu K10 Xia, He K49 Jinglong, Liu OR30 Yongzhong, He K188 Min, Liu P30 Mingyan, Hei K48 Qingle, Liu OR09 Wencheng, Liu K04 Danhong, Song K147 Wenwu, Liu K104 Juan, Song K173 Xinying, Liu K05 Li, Sun K172 K209 Xuehua, Liu OR25 Lin, Sun K171 Yaling, Liu K06 Xuejun, Sun PL9 Yi, Liu K07 Ying, Long K35 T Min, Lou OR20 Zhongquan, Tang K179 Jiangjiang, Lu K44 Hengyi, Tao PL3 Peigang, Lu P12 K45 Jinzhong, Teng K163 Xiaoxin, Lu P31 Joseph, Ting K87 Chaoqun, Tu K17 M Linlin, Ma K43 V Yongxin, Ma K41 K42 Johannes, von Reumont P50 Yujie, Ma K39 Yongjun, Mai K38 W Yoshihiro, Mano PL4 Chia-Ti, Wang P39 P48 Fangguan, Mao K36 Gang, Wang PL6 P09 Lijian, Mao P04 Guohua, Wang P03 Daniel, Mathieu PL8 Guozhong, Wang K162 Xiufang, Mei P11 Hongjuan, Wang P45 Ying, Mei K23 Jun, Wang P17 Cristian, Melián P40 P41 Liping, Wang P07 K67 Bing, Meng K40 Min, Wang K161 Juan, Meng K28 Peidong, Wang OR22 OR24 Jun, Meng K33 Qinghong, Wang P35 Qiusha, Wang K158 K159 K160 N Rongzhen, Wang K134 Yulin, Nie K26 Shengzhi, Wang K157 Ko-Chi, Niu PL7 Shuzhen, Wang K156 Shyanher, Wang P49 P Wei, Wang K155 Fuqiong, Pan OR15 Wenjie, Wang K72 Hekui, Pan K138 Wenlan, Wang OR19 Jiexiang, Pan K29 K31 Xiaohong, Wang K154 Lin, Pan K127 Xiaoli, Wang P23 Shuyi, Pan OR18 K25 Yalin, Wang K153 Xiaorong, Pan K136 Yong, Wang OR27 Xiaowen, Pan P01 P16 Youbin, Wang OR26 Jinjun, Pang K37 Youcun, Wang K152 Huiping, Peng K63 Yuhua, Wang K164 Ya, Peng K122 Chengwen, Wei K195 Zhaoyun, Peng K65 Fen, Wei K182 Zhengrong, Peng OR29 Qibiao, Weng OR23 K198 Lianhua, Wu K206 K207 Q Qiaoning, Wu K194 Yan, Qi K178 Ruilan, Wu K202 Chunling, Qian K177 Shuanggui, Wu K203 Haiying, Qiu K146 Xiuyun, Wu K71 Jun, Qu K27 Yali, Wu K204 Cuixia, Que K196 Yanqiu, Wu K205 Lei, Que K185 K186 Zhide, Wu K192

R X Cunge, Ren K176 Hongzhi, Xia K208 Ping, Ren K169 Haibin, Xiang K139 K193 Pingtian, Xiao P24 K190 K191 S Jinxiang, Xie K180 Jingyi, Shao K175 Qiuyou, Xie P02 Paul J., Sheffield B2 Rong, Xie K189 Hansheng, Sheng K174 Xiaoping, Xie P27 Chunmei, Shi K197 Zhihui, Xie K187 Raymond C, Shields P43 Lili, Xu K01 Limin, Xu K85

Y Kazuyoshi, Yagishita OR04 Maoxing, Yan K129 Xiaomin, Yan K184 Chen, Yang P36 Haiping, Yang K151 Jianhua, Yang K183 Jingfu, Yang P05 Jinhua, Yang K94 Lin, Yang K119 K120 Ping, Yang K133 Qunfang, Yang K118 Quyun, Yang K201 Wenping, Yang K117 Xiaolin, Yang K116 Xin, Yang K115 Yuanbi, Yang K128 Yujia, Yang P06 P26 Zheng, Yang K114 Yue, Yao K91 Shifu, Yin K113 Daoyuan, Yu K110 Guoping, Yu K08 Ning, Yu K112 Qiuhong, Yu P22 Ronghao, Yu P29 P46 Xuelai, Yu K126 Youjie, Yu K111 Jing, Yuan K121 Lina, Yue K210

Z Shaoxia, Zeng K109 Denghua, Zhang K93 Dongyun, Zhang K75 Hechun, Zhang K107 Jian, Zhang K137 Jin, Zhang P37 Li, Zhang K166 Ludi, Zhang K105 Wenli, Zhang K168 Yan, Zhang K103 Yi, Zhang K102 Yubao, Zhang K145 Hui, Zhao K101 Chenggang, Zheng K100 Ming, Zhong K99 Qiaofen, Zhong P32 Dun, Zhou K98 Hongtu, Zhou K95 K96 K97 Kunru, Zhou K135 Shurong, Zhou OR16 K106 Xuming, Zhou K108 K148 K149 Yihong, Zhou K144 Hourong, Zhu K143 Jianming, Zhu K140 K141 K142

Stránka 1: [1] Komentář [L7] Lenvov 19.10.2008 4:39:00

正确的称呼？

Stránka 1: [2] Odstraněno Lenvov 2.2.2009 3:03:00

Table of Contents

Welcome Message from Conference President ...... 1 Welcome Message from ICHM Executive President...... 2 Committees ...... 2 Conference Information ...... 3 Scientific Information...... 5 Detailed Program (Oct. 27) ...... 8 Detailed Program (Oct. 28) ...... 12 Detailed Program (Oct. 29) ...... 16 大会主席致辞 ...... 19 ICHM 执行主席致辞 会议组织结构 ...... 21 会议基本信息 ...... 22 学术信息 ...... 23 详细日程 (10 月27日)...... 25 详细日程 (10 月28日)...... 28 详细日程 (10 月29日)...... 31 Poster Program 壁报日程 ...... 33 Acknowledgement 鸣谢 ...... 48 Exhibitors 展商 ...... 50 论文集 Abstracts ...... 50 Index 索引 ...... 56 Konec oddílu (další stránka)

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Welcome Message

Dear friends and colleagues,

Welcome to Beijing for the 16 th international Congress on Hyperbaric Medicine (ICHM2008).

The world has witnessed the rapid economic and social development in China in the recent years. The HBO medicine is developing and advancing together with the economic growth. In particular, it has got inspiring achievements in China in the basic and clinical research of HBO therapy in the treatment of delayed neuropsychologic sequelae, ischemic cerebral vascular diseases and high altitude diseases. Chinese health professionals are willing to share our experience with our colleagues around the world.

The ICHM2008 will be a summit meeting for all leading specialists in the field of h [l1] yperbaric and u[l2] nderwater Medicine, and a grand gathering for basic researchers and clinical experts from all over the world. World-renowned experts will give lectures during the congress. I am sure their speeches must be both educational and interesting.

We also invite your family and friends to come with you. We have arranged special tours for your accompanying persons. I believe they will enjoy their stays in Beijing when you have busy scientific schedule at meeting.

A rich cultural and historic heritage, accumulated through the passage of time, adds an extraordinary aura to Beijing. The five dynasties that chose Beijing as their capital have left abundant antique treasures. Travelers would marvel at every turn on a tour to this hottest destination of the world today: from the grandiose Forbidden City to the gorgeous Great Wall, and from the labyrinth-like hutong to other worldly royal mausoleums.

Wish you a good stay in China.

Prof. Gao Chunjin Congress President [l3] 16th International Congress on Hyperbaric Medicine Konec oddílu (další stránka)

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Welcoming Address to the Participants of the XVI-th Congress of the International Congress on Hyperbaric Medicine (ICHM).

Exactly 45 years ago, in 1963, the First Congress of the ICHM took place in the Surgical Clinic of the University Hospital, the Wilhelmina Gasthuis in Amsterdam, the Netherlands. Professor Ite Boerema, the “father of Hyperbaric Medicine” and professor of Surgery and his Staff had invited many colleagues from all over the World to discuss the first results of the application of Hyperbaric Oxygen Drenching, as Boerema used to call it, in a variety of indications.

The most prominent indications at that time were Infectious diseases mainly Anaerobic Infections, Coronary Infarction, Experimental Cancer Therapy in a combination with Chemotherapy and Hyperthermia. Also the use of HBO in Carbon Monoxide poisoning, both experimental and clinically was discussed. Much attention was given to Physiological and Pharmacological problems and Oxygen Toxicity, with this new mode of therapy. Cardiovascular Surgery in combination with HBO and Hypothermia, the first and for some time the only indication, was also a leading subject. Both experimental and clinical results were reported. Building problems of chambers and Safety were also not forgotten. Participation to that Congress was strictly on invitation by Boerema himself, at that time well thought of, but fortunately the one and only time that this happened in our history. The participants came from the USA, the UK, France, Australia and South Africa. In a historical review Jack Jacobson II of New York started with mentioning the Diving Bell of Aristotle, used in 332 BC by Alexander the Great in his siege of Tyre. So our history of diving and hyperbaric medicine goes back almost as far as medicine in general.

From that time on regular Congresses were held at a four-yearly (until 1981, Moscow) and later three- yearly interval. The first time that the Congress moved to Asia was in 1969 when we met in Sapporo, Japan. In 1993 the Congress gathered in China, in Fuzhou under the chairmanship of the late professor of Cardiac Surgery, Wen Ren Li. We are very happy that we are back in China today thanks to the efforts of our present chairman, professor Gao Chunjin and his whole team of the Chinese Medical Association (mrs. Chenchen) and the Chinese Hyperbaric Medical Society.

I must say that I have been much impressed in the foregoing two years by the very solid and precise preparations of the Organizing Committee. Nothing has been left to chance. The same has been noticed by the whole world in the Organisation of the Olympic Games and the Paralympic Games just before our Congress this year. On behalf of the Executive Committee of the ICHM and all participants here, I will extend my sincere thanks and admiration to prof Gao Chunjin and his staff for this excellent organization.

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We all look forward to the coming days, both professionally and socially. It is always a pleasure to meet old friends again in these nice surroundings. There will be also much work to do. The Executive Committee will lose two of its long standing members, our Secretary-Treasurer Fred Cramer and our Executive Director, Dirk Jan Bakker. After many years of serving you and because of our ages we will resign during this Congress and we will choose new members during the Governors Luncheon. I myself will stay involved in matters of the Congress but not any more in an official position. From this point I want to thank Fred very sincerely for his never ending efforts to advertise the Congress on various occasions, to solicit for new Membership and finding sponsoring for the Newsletter, which was really not an easy task. It will be difficult to replace him.

Well ladies and gentlemen, dear colleagues and friends, I am convinced that we will have an excellent and rewarding time here in the magnificent historical city of Beijing, both scientifically and socially. I wish you all a very pleasant and happy stay.

Thank you very much for your attention.

D.J.Bakker, MD PhD h.t. Executive Director of the ICHM. Konec oddílu (průběžné)

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Committees

President: Gao Chunjin, Department of Hyperbaric Medicine, Chaoyang Hospital, Capital Medical University

Vice Presidents: Pan Xiaowen, Hyperbaric Medicine Center, Navy General Hospital Tao Hengyi, Department of Diving Medicine, Second Military Medical University

Secretary General: Tao Hengyi, Department of Diving Medicine, Second Military Medical University

Chair of Logistic Committee: Wang Peisong, Department of Hyperbaric Medicine, Affiliated Hospital to Qingdao University Medical School

Co-Chair of Logistic Committee: Weng Qibiao, Department of Hyperbaric Medicine, Zhujiang Hospital, South Medical University

Chair of Scientific Committee: Yi Zhi, Shenzhen People’s Hospital, Guangzhou

Co-Chair of Scientific Committee: Wang Gang, Affiliated Hospital, China Medical University, China

Members: Joseph Ting (Maylasia), Pan Shuyi, Huang Xiang, Hu Huijun, Li Zhuo, Yang Lin, Lu Yan, Zhang Yue, Xu Weigang konec oddílu Congress Information

1. Registration & Information Center Venue: level 2, Beijing International Convention Center Add: No.8, Beichendong Road, Chaoyang District, Beijing, China

Opening Hours: Oct.26 (Sunday) Oct. 27 (Monday) Oct. 28 (Tuesday) Oct. 29 (Thurday) 09:00-18:00 8:00-17:00 8:30-17:00 8:30-11:30

On-site Registration fee: Non Member ￥5,500 Accompanying Person ￥2,000.00 * Member ￥5,000 * *Nurse & Trainee ￥2,500.00 * Member is ICHM live member. * * A copy of student ID or a verification letter from his or her supervisor are requested.

2. Participant Identification and Entitlements Please wear your Congress badge during the Conference. Red Delegate Yellow Accompanying person Green Exhibitor Blue Staff Red Ribbon Invited Speaker / Chair / Committee Members

3. Congress Official Language English

4. Coffee/Tea Break Coffee, tea and snacks will be provided Oct. 27 Oct. 28 Oct. 29 10:40-11:00am 10:00-10:30am 10:00-10:30am 15:00-15:30pm 15:00-15:30pm 15:00-15:30pm

5. Meals Oct. 26 Oct. 27 Oct. 28 Oct. 29 Lunch N/A Beijing Continental Beijing Continental Beijing Continental Grand Hotel Grand Hotel Grand Hotel Dinner Beijing Continental Welcome reception Conference Dinner Grand Hotel (Laoshe Theater)

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6. Exhibition Area: Exhibit Hours: 09:00-17:00 Oct. 27 and 28, 09:00-12:00 Oct. 29, 2008

7. Accommodation For any inquiry about the hotel, please go to the Registration/Information Center (Level 2, Beijing Continental Grand Hotel). 8. Social Program

Oct. 26 Acrobatics with dinner Price: 300 yuan The usual reaction to a Chinese acrobatics show is something like “Whoa! That was so…” The contortions and balancing acts are quite incredible. Audients will be at the edge of their seats through most of the show saying “don’t fall, don’t drop it!” and incredibly, they never do.

Oct. 27 Welcoming banquet (Roast duck dinner and Peking opera) Free to all fully-paid participants and accompanying persons. Additional ticket: 340 yuan Audients will be led to a downtown theatre for Peking Operas, face changing opera and etc. Beijing Roast duck and other traditional Peking dishes will be served as well.

Oct. 28 Conference Banquet Free to all participants and accompanying persons. Additional ticket: 200 yuan

Oct. 29 Olympic Venue visits/Hospital visit The organizing committee invites you to some must-go Olympic venues like Bird's Nest and Water Cube. Free to all participants.

Oct. 29 The Night of Beijing Price: 425 yuan The show is a dinner / theatrical performance unsurpassed in Asia. A luxurious artistic feast will be presented in this delicately decorated theatre that you can hardly resist. The brilliant Beijing Opera, the elegant Chinese dance, the magnificent acrobatics and marvelous Chinese Kongfu are well integrated with the beautiful costumes and settings. The pleasing music and spotlights and other modern stage arts will be a real shock of beauty and unforgettable experience to you in China. What’s more, the delicious Beijing food served in the theatre will make your evening more enjoyable.

9. Accompanying person’s tour – Exciting shopping tour Date: Morning, Oct. 29, 2008 A tour voucher is included in the accompanying person’s registration package. Please bring the voucher to the Tour Desk of Registration Area at level 2, Beijing International Convention Center to sign your name for and learn more information about the tour.

10. Post-Congress Inquiry CMA Meeting Planner, Chinese Medical Association Add: 42 Dongsi Xidajie, Beijing 100710, China Tel: +86 10 8515 8148 Fax: +86 10 6512 3754 Email: [email protected] konec oddílu

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Scientific Information

1. Speakers: Please hand in your MS PowerPoint slides at least one hour before your presentation to the conference room staff.

2. Session Chairs: We suggest you meet the staff inside the meeting room in case of any adjustments in the program or speaker absences.

3. Poster: Poster area: Exhibit Hall 2, Level 1, Beijing International Convention Center Language: English Presentation Time: Oct. 27 Oct. 28 Oct. 29 10 ：40 – 11 ：00 10 ：00 – 10 ：30 10 ：00 – 10 ：30 15 ：00 – 15 ：30 15 ：00 – 15 ：30 Your poster will be displayed throughout the entire congress. Please stand by your poster and make presentation during the above mentioned timeslots. Please hand in your poster to the Poster Desk at the Registration Area and take off your poster 12:00-12:30, Oct. 29. konec oddílu

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Detailed Program Oct. 27 8:30-9:00 Opening Ceremony Chairs: Zhi Yi

9:00-10:40 Invited Session 1 Chairs: Xiaowen Pan, Jordi Desola 9:00-9:50 B1 The use of Hyperbaric Oxygen in Necrotising Soft Tissue Infections: A Historical Perspective with an Update anno 2008 D. J. Bakker, MD, PhD. Amsterdam, the Netherlands 9:50-10:40 B2 Role of Tissue Oximetry in Assessment of Problem Wounds Paul J. Sheffield, PhD; International ATMO, Inc, Nix Wound Healing Center, San Antonio, Texas, USA

10:40-11:00 Break

11:00-12:00 Invited Session 2 Chairs: Peisong Wang, Ko-Chi Niu 11:00-11:30 PL1 The Indication of Hyperbaric Oxygen Therapy and for Orthopaedics in Japan Mahito Kawashima, Takashi Yamaguchi, Kawashima Orthopaedic Hospital, Japan 11:30-12:00 PL2 The Use of “Deep Stops” In Preventing Precordial Detectable Doppler Bubbles in Recreational Scuba Divers Frans J. Cronjé, MBChB, MSc, DAN Southern Africa;

lunch

13:30-15:00 Invited Session 3 Chairs: Paul J. Sheffield, Qibiao Weng 13:30-14:00 PL3 An Overview of Diving and Diving Medicine in China Tao Hengyi, Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China 14:00-14:30 PL4 DCS incidence of commercial dive works in Japan and Evaluation of the profile on multi-level diving by integration of nitrogen accumulation in each body tissue Yoshishiro Mano, Hyperbaric Center, Tokyo Medical & Dental University Hospital, Japan 14:30-15:00 PL5 Traumatic Spinal Cord Injuries – is There a Role for HBO? Jochen Freier, MD, Consultant für Diving and Hyperbaric Medicine (EDTC), Anaesthesiology and

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Intensive Care, Vice President of the German Society für Hyperbaric Medicine (GTUEM), Tagesklinik Hofheim, Germany

15:30-17:15 Free Paper Session 1 Charis: Pingtian Xiao, Mano Yoshihiro 15:30-15:45 OR01 HBO and cytostatic chenotherapy Christtian Heiden, German Professional Soc. Hyperb. Chambers 15:45-16:00 OR02 Hyperbaric Oxygen: Does it Promote Cancer Recurrence or Metastasis? An Update John Feldmeier, University of Toledo Medical Center|Radiation Oncology Department; Toledo Radiation Oncology 16:00-16:15 OR03 Hyperbaric Oxygen for Complications of Oncological Treatment of Paediatric Patients Michal Hajek, Centre of Hyperbaric Medicine, Municipal Hospital of Ostrava, Czech Republic 16:15-16:30 OR04 Hyperbaric Oxygen may accelerate tumour-bearing mice to death Liu Qingle, Changhai Hospital, the Second Military Medical University, Shanghai, China 16:30-16:45 OR05 Hyperbaric oxygenation (HBO) in mediastinitis treated with an open mediastinum after cardiac surgery Jacek Kot, National Center for Hyperbaric Medicine, Medical University, Gdansk, Poland 16:45-17:00 OR06 Normobaric and hyperbaric oxygen therapy for migraine and cluster headache Michael Bennett, Dept. Diving and Hyperbaric Medicine, Prince of Wales Hospital, Sydney, Australia 17:00-17:15 OR07 Hyperbaric oxygen therapy induces antiinflammation and antipyresis in experimental studies Lin Mao-Tsun, Department of Medical Research and Department of Hyperbaric Oxygen therapy, Chi- Mei Medical Center, Taiwan, China

17:15-17:30 Satellite symposium

Konec oddílu (další stránka)

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Oct. 28 8:30-10:00 Invited Session 4 Chairs: Jochen Freier, Qingle Liu 8:30-9:00 PL6 Hyperbaric Oxygen Therapy in Acute Traumatic Peripheral Ischemic Diseases Wang Gang M.D. Ph.D., Emergency Dept & Hyperbaric Oxygen Dept., First Affiliated Hospital, China Medical University, Shenyang, China 9:00-9:30 PL7 The Application of Hyperbaric Oxygen Therapy in Critical Disorders Niu Ko-Chi, Department of Medical Research and Department of Hyperbaric Oxygen, Chi Mei Medical Center, Tainan, Taiwan, China 9:30-10:00 PL8 Hyperbaric Medicine: an European Perspective Daniel Mathieu, Centre Hyperbare, Hôpital Calmette, Centre hospitalier Régional et Universitaire, Lille, France

10:00-10:30 Break/Poster

10:30-12:00 Free Paper Session 2 Chairs: Joseph Ting, Shurong Zhou 10:30-10:45 OR08 Measurement of plasma nitrotyrosine and nitrite after a single treatment with hyperbaric oxygen in healthy human subjects RENU AGNIHOTRI, SABA UNIVERSITY SCHOOL OF MEDICINE 10:45-11:00 OR09 HBO in Tokyo Medical and Dental University in 2007 and The Effects of HBO on Soft Tissue Injury in Sports Activity Kazuyoshi Yagishita, Hyperbaric Medical Center/ Orthopaedic Surgery, Tokyo Medical and Dental University 11:00-11:15 OR10 adjunctive use of hyperbaric oxigenacion (HBO2) in the managment of cerebral hemorrhage in a pregnant patient: a case report Alberto Chavez, Hospital Angeles Metropolitano 11:15-11:30 OR11 Necropsic Findings Of Syndrome Of Intratoracic Hyperpressure In A Young Diver And A Decompression Accident After A Deep Scuba Diving In Veteran Diver (Two Particular Cases) John M Batle, MEDISUB Hyperbaric & Underwater Research Institute Palma de Mallorca SPAIN 11:30-11:45 OR12 24 cases of neurological decompression illness - 14 months of a single center experience

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Jorge Calderón, hospital Ancud|Pontificia Universidad Católica de Chile 11:45-12:00 OR13 Effect of Hyperbaric Oxygen Treatment on Traumatic Brain Injury Rat by Magnetic Resonance Imaging Huang Xiang, Bejing Shunyi Hospital of China Medical University, Beijing, China

13:30-15:00 Free Paper Session 3 Chairs: Frans Cronje, Fuqiong Pan 13:30-13:45 OR14 The effect of hyperbaric oxygenation on diabetic foot ulcers (the HODFU-study). A single centre, prospective, randomised, double-blind placebo-controlled parallel-group study Christer Hammarlund, Helsingborg Hospital, Sweden|Lund University Hospital, Sweden|Malmoe University Hospital, Sweden 13:45-14:00 OR15 First-aid of 12 Gas Gangrene Cases Caused by the Earthquake Injuries by Use of Hyperbaric Oxygen Pan Fuqiong, Sichuan Provincial Hospital, Chengdu, China 14:00-14:15 OR16 A survey on HBO treatment of traumatic brain injury this century in China—A important evidence of HBO improve curative effect and prognosis of Brain injury Zhou Shurong, Zhou Shurong First Affiliated Hospital of Nanjing Medical University, Nanjing, China 14:15-14:30 OR17 Clinical analyses of 429 Cases of Acute CO Poisoning Li Zhuo, Beijing Chaoyang Hospital, Beijing, China 14:30-14:45 OR18 Review of Current situation on acute carbon monoxide poisoning in china Pan Shuyi, Navy General Hospital, Beijing, China 14:45-15:00 OR19 Effect of HBO on endogenous neural stem cells in rat models of acute CO poisoning Wang Wenlan, Department of hyperbaric oxygen treatment center, School of Aerospace Medicine, Fourth Military Medical University, Xi’ an, China

15:00-15:30 Break

15:30-17:15 Free Paper Session 3 Chairs: Daniel Mathieu, JIndong Lu 15:30-15:45 OR20 Hyperbaric oxygen treatment attenuated the decrease in regional glucose metabolism of rats subjected to focal cerebral ischemia: a high resolution positron emission tomography

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study M. LOU, Department of Neurology, the Second Affiliated Hospital, Zhejiang University, School of Medicine, Hangzhou, China 15:45-16:00 OR21 Treatment of micro-found drilling cranium and hyperbaric oxygen on 94 cases of patients with extradural hematoma Cheng Jincheng, 123th Hospital of People’s Liberation Army 16:00-16:15 OR22 The effect of hyperbaric oxygen on the osteoporosis rats Wang Peisong, Department of hyperbaric oxygenation, The affiliated Hospital of Qingdao University Medical College, Qingdao, China 16:15-16:30 OR23 Trial of cabin-oxygen-concentration distribution of single oxygen pressurized module Weng Qibiao, Zhujiang Hospital attached to Southern Medical University, Department of Hyperbaric oxygen, Guangzhou, China 16:30-16:45 OR24 Clinical Research on Effect of HBO plus Electric Stimulation for Treatment the Cerebral Resuscitation Wang Peidong, Chinese HBO Medicine Association Research Center of Brain Recovery, Nanjing, China 16:45-17:00 OR25 Effect of Hyperbaric Oxygen Treatment on the content of VEGF, HIF-1,CX43 in skin flaps of rabbits Liu Xuehua, Department of Hyperbaric Oxygen, Chaoyang Hospital, Capital Medical University 17:00-17:15 OR26 Hyperbaric Oxygenation Preconditioning Induce Skin Flap Ischaemia Tolerance in Rat Model Wang Youbin, Plastic Surgery Department of Peking Union Medical College Hospital, Beijing, China

Konec oddílu (další stránka)

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Oct.29 8:30-10:00 Invited Session 5 Chairs: Christtian Heiden, Qiang Wang 8:30-9:00 PL9 The Pretective Effect of Hyperbaric Oxygen Preconditioning on ischemic/hyoxia Injury Xuejun Sun, Department of diving medicine, Second Military Medical University. Shanghai, China 9:00-9:30 PL10 Disruption of Viral Pathogenesis Following Patient Exposure to Hyperbaric Environments with Altered Inspired Gas Mixtures Stephen D. Guthrie, MD, PhD, Designed Altobaric  Research Institute, Livonia, Michigan, USA 9:30-10:00 PL11 Carbon Monoxide Poisoning Clinical Findings, Common Errors, And Early diagnose: A prospective analysis of 2900 cases Jordi Desola, MD, PhD, Hyperbaric Therapy Unit of Barcelona, Barcelona, Spain

10:00-10:30 Break/Poster

10:30-11:30 Free Paper Session 5 Chairs: Yunping Ji 10:30-10:45 OR27 The Effect of Hyperbaric Oxygen on Histopathology and Coagulation-fibrinolysis Systems in Rabbits with Steroid-induced Avascular Necrosis of the Femoral Head Wang Yong, Department of Hyperbaric oxygen, Fuxing Hospital, Capital Medical University, Beijing, China 10:45-11:00 OR28 Hyperbaric oxygen preconditioning protects against traumatic brain injury at high altitude Hu Shengli, Department of Neurosurgery, Southwest Hospital of the Third Military Medical University, Chongqing, China 11:00-11:15 OR29 The effect of hyperbaric oxygen on the proliferation and death of Nasopharyngeal Carcinoma Cells and its mechanisms Peng Zhengrong, Xiangya Hospital, Central South University, Changsha, China 11:15-11:30 OR30 Effect of Hyperbaric Oxygenation (HBO) on Unilateral Spatial Neglect ( USN) Liu Jinglong, Heilongjiang Rehabilitation Hospital, Ha’erbin, China

11:30-12:00 Closing Ceremony Chairs: Zhongquan Tang Closing Remark, Hengyi Tao [L4] Konec oddílu (další stránka)

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大会主席致辞

尊敬的各位同道、各位朋友：

首先，我谨代表大会组织委员会欢迎各位同仁前来北京参加第十六届国际高气压医学学术会议暨第十七届全国 高压氧医学学术会议。

本次会议是国际高气压医学会自 1993 年以来的[l5] 第二次在中国举行会议 [l6] ，是我国高压氧医学蓬勃发展、加 强国际交流的结果。

近几年，世界见证了中国经济和社会的高速发展，高压氧医学也伴随经济进步的脉搏向前超越。特别是应用高 压氧治疗一氧化碳中毒迟发性脑病，缺血性脑血管疾病及高原疾病领域，中国在基础和临床研究方面取得了令人鼓 舞的成绩。中国的医务工作者期盼与世界分享我们的经验，同时也向世界各国的同道学习、交流。

本届大会将是高压氧医学和潜水医学权威学者的一次峰会，也将成为各国研究者及临床专家的盛会。多 为（位 ）享有盛誉的学者欣然接受邀请到会并作精彩生动的报告。

金秋十月，阳光和煦，微风送爽。继2008年北京奥运会和残奥会之后，古都北京到处充满了新的生机和活力。 我们特地选择了北京国际会议中心作为大会会场，它毗邻奥运村，与 “鸟巢 ”咫尺相隔。会后，感兴趣的代表可参观 奥运赛场。

再次感谢您的光临，并希望各位在北京参会期间愉快、有所收 货（获） 。

高春锦 第十六届国际高气压医学学术会议主席 中华医学会高压氧医学分会主任委员 Konec stránky 国际高气压医学会国际高气压医学会主席致辞主席致辞

45年前，即 1963年，第一届国际高气压医学学术会议于

Stránka 1: [3] Odstraněno Lenvov 2.2.2009 3:03:00 45年前也就是1963年在

Stránka 1: [4] Odstraněno Lenvov 2.2.2009 3:03:00 荷兰阿姆斯特丹Wilhelmina Gasthuis大学医院外科部隆重召开。受“高压氧医学之父”、 外科教授Ite Boerema和他的同事之邀，

Stránka 1: [5] Odstraněno Lenvov 2.2.2009 3:03:00 各国同仁积极参会并共同探讨

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Stránka 1: [6] Odstraněno Lenvov 2.2.2009 3:03:03:03:00000 了最初被Ite Boerema教授称之为“高压

Stránka 1: [7] Odstraněno Lenvov 2.2.2009 3:03:00 在不同病种中的应用及疗效

Stránka 1: [8] Odstraněno Lenvov 2.2.2009 3:03:00 用于不同症状的初步效果

Stránka 1: [9] Odstraněno Lenvov 2.2.2009 3:03:00 ，“高压纯氧浸透法”是Ite Boerema教授最初对高压氧疗法的称呼。

Stránka 1: [10] Odstraněno Lenvov 2.2.2009 3:03:00 当时高压氧疗法最主要的

Stránka 1: [11] Odstraněno Lenvov 2.2.2009 3:03:00 适应症为以厌氧菌感染为主的感染性疾病，冠状动脉梗塞，结合化

Stránka 1: [12] Odstraněno Lenvov 2.2.2009 3:03:00 和高温疗法治疗癌症。大会讨论了

Stránka 1: [13] Odstraněno Lenvov 2.2.2009 3:03:00 各种传染病，例如 厌氧菌感染 ，冠状动脉梗塞及试验性癌症疗法。也对

Stránka 1: [14] Odstraněno Lenvov 2.2.2009 3:03:00 应用高压氧治疗一氧化碳中毒 的临床和基础研究，

Stránka 1: [15] Odstraněno Lenvov 2.2.2009 3:03:00 ，科研和临床 （都）予以了讨论。

Stránka 1: [16] Odstraněno Lenvov 2.2.2009 3:03:00 同时，大家还也很关注

Stránka 1: [17] Odstraněno Lenvov 2.2.2009 3:03:00 生理、药理以及氧中毒

Stránka 1: [18] Odstraněno Lenvov 2.2.2009 3:03:00 问题予以了特别关注。大会的首要议题还包括探讨高压氧结合

Stránka 1: [19] Odstraněno Lenvov 2.2.2009 33:03:00:03:00 低温疗法辅助进行心血管手术的临床与基础研究，这在一段时期内曾经是高压氧唯一的也是最主要的适应症， 除此之外，是第一个用途，在一段时间也是惟一的用途，并且是惟一的主题。临床和试验的结果都有所报道。建设 氧仓和安全问题也没有 忘记（被忽略）。 那时参会者都是收到了 Boerema 教授本人的要求（邀请），（那时）是经过深思熟虑的， 但是幸运的是，这是 惟一一次也是最后一次（在我们的历史上是空前绝后的） 。参会者来自美国，英国，法国，澳大利亚和南非。在历 史回顾中，来自纽约的Jack Jacobson II首先提到了公元前332年亚历山大大帝在提尔城之围中用到了亚里士多德的潜水钟。如此看来，潜水和高气压的历 史与医学史一样古老。 从那时起每四年召开一次会议，直到1981年莫斯科会议以后，变为每三年举办一次。国际高压氧医学会首次亚 洲会议是于1969年在日本札幌举行。1993年在中国心脏外科专家李温仁教授的主持下，我们首度在中国福州举办

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了国际高压氧医学学术会议。我们非常高兴今天再次来到中国举办会议，感谢今天的大会主席高春锦教授，中华医 学会及中华医学会高压氧医学分会所做的努力。 在刚刚结束的奥运会和残奥会中，我们看到了优秀的组织工作。而在过去的两年中本次会议组委会所作的具体 而细致的准备工作同样让我印象深刻。 我代表国际高压氧医学会执行委员会和各位参会者，向高春锦教授和她的团队表示衷心的感谢。未来的几天里，

Stránka 1: [20] Odstraněno Lenvov 2.2.2009 3:03:00 我们无论从学术上还是活动上都期待接下来的几

Stránka 1: [21] Odstraněno Lenvov 2.2.2009 3:03:00 全体代表将共享这次盛会

Stránka 1: [22] Odstraněno Lenvov 2.2.2009 3:03:00 能在这样美好的

Stránka 1: [23] Odstraněno Lenvov 2.2.2009 3:03:00 。 未来的高气压医学任重而道远，由于年龄原因，在为各位服务多年后，我们的财务秘书 [L7]

Stránka 1: [24] Odstraněno Lenvov 2.2.2009 3:03:00 Fred Cramer 和执行主席 Dirk Jan Bakker 将要退出执行委员会。

Stránka 1: [25] Odstraněno Lenvov 2.2.2009 3:03:00 由于年龄原因，在为各位服务多年后，我们将要退出，在委员午餐中选出

Stránka 1: [26] Odstraněno Lenvov 2.2.2009 3:03:00 新的成员将在委员会会议中选举产生。我本人

Stránka 23: [27] Odstraněno Lenvov 19.10.2008 7:15:00 HBO in Tokyo Medical and Dental University in 2007 and The Effects of HBO on Soft Tissue Injury in Sports Activity Kazuyoshi Yagishita, Nobuo Yamami, Seiichiro Togawa, Yoshihiro Mano Hyperbaric Medical Center, Tokyo Medical and Dental University, Tokyo, Japan

Objective: In our university hospital, multiplace chamber including 3 rooms and the capacity of 16 patients was set up in 2001. In 2007, 7970 times hyperbaric oxygen therapy (HBO) in 958 patients were performed, which is the most patients number in one institute in a year in Japan. The purposes of this study were to report the diseases and the patients performed with HBO in our hospital last year. The effects of HBO on crush injury and compartment syndrome were well documented by many authors, and randomized controlled studies revealed the effects of HBO on reduction of necrosis and edema. We now perform HBO aggressively to soft tissue injury including compartment syndrome, ankle sprain, knee ligament injury, and muscle strain. The purposes of this study were also to investigate the effect of HBO on soft tissue injury in sports activity. Patients and Methods: 7970 times HBO in 958 patients were performed in our hospital in 2007. The number of the patients and HBO times were evaluated. In 180 patients with soft tissue injury in sports activity, visual analog scale (VAS) scores and recovery time to previous sports activity were evaluated. Results: The number of the patients and HBO times in decompression illness were respectively 374 patients and 764 times, 87 patients and 934 times in sudden deafness, 34 patients and 740 times in peripheral vascular disorder including diabetes and arteriosclerosis obliterans, 47 patients and 1046 times in osteomyelitis, 180 patients and 865 times in soft tissue injury related sports activity, 90 patients and 1644 times in myelopathy and radicuropathy, 19 patients and 220 times in carbon monoxide poisoning, and 18 patients and 259 times in radiation-induced cystitis. In patients with soft tissue injury in sports activity, VAS scores improved compared between pre and post 2-hour HBO. In patients with 2 nd grade medial collateral ligament injury of the knee in HBO group, recovery acceleration to previous sport activity was observed. Conclusion: In HBO procedure in our university in 2007, no major patient troubles and no chamber problems were recorded. The effects of HBO on soft tissue injury were strongly suggested in this study.

Stránka 25: [28] Odstraněno Lenvov 19.10.219.10.2008 008 7:15:00

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OR09 Hyperbaric Oxygen may accelerate tumour-bearing mice to death Qingle LIU, Chenggang Zheng, Xiaohua Hang Changhai Hospital, the Second Military Medical University, Shanghai, China

Objective: To explore the influence of hyperbaric oxygenation (HBO) on life-span in tumour-bearing mice. Method: Male Balb/c mice were randomly divided into celiac-tumour group: inoculating tumour cell into abdominal cavity of the animal, celiac-tumour-HBO group: inoculating tumour cell into abdominal cavity and then exposing to hyperbaric oxygen, back-tumour group: inoculating tumour cell under skin of the back of the animal, back-tumour-HBO group: inoculating tumour cell under skin of the back of the animal and then exposing to hyperbaric oxygen. There are 7 mice in each group. After s-180 tumour cell was translated to mice HBO was given once a day in concerned group. The life-span and skin ulcer were observed. Results: The life-span is 27.57 士4.4693 days in celiac-tumour-group, 24 士2.9439 days in celiac-tumour-HBO group, 63.43 士21.844 days in back-tumour group, and 35.14 士8.934 days in back-tumour-HBO group. The life-spans in celiac-tumour group and in celiac-tumour-HBO group are different, but P value is 0.133204; the life-spans in back-tumour group and in back-tumour-HBO group are significant and P value, 0.009002. Conclusion: The life-span of mice in back- tumour-HBO group was shorten by exposing HBO, and life-span in celiac-tumour-HBO group is tendency to be shorten by HBO exposure. The ulcer formation is postponed by HBO in back-tumour-HBO group.

Stránka 30: [29] Odstraněno Lenvov 19.10.2008 7:43:00 The medical oxygen pressurized cabin which is convenient and suitable for patients with different diseases and ages has been widely used for decades. Meanwhile, it is accepted by a majority of hospitals. To select appropriate input/output of oxygen, structure and washing method is beneficial to oxygen concentration elevation and distribution in cabin, because pure oxygen is used as medium for compression. Three ways of oxygen input/output(on the top, middle and bottom of the cabin), three states of the cabin(with a bed, with a bed and a patient, without a bed and a patient) and three ways of washing method(continued washing method with boosting pressure, washing method of boosting pressure of the gap of the door, boosting pressure directly without cabin- washing) was adopted in our trial. To detect oxygen distribution during the procedure of elevated, stable and decreased pressure, nine sampling outlets which located on the top, middle and bottom of cabin were set. Delivery pressure in cabin is 0.1 MPa in the meter. Experimental session lasted for 80 minutes. Process of experiment accorded to the rule of Chinese Medical Association—HBO branch. Measurement range of digital oxygen analyzer is 0-100%. Interval time of sampling is 2 minutes. Influential factor of oxygen distribution in cabin-Our research suggests that main influential factor of oxygen distribution are broadside stretcher and input/output location of oxygen at the bottom of cabin. Oxygen distribution of the structure that broadside stretcher combined with input/output oxygen at the bottom of cabin is as follow: 90% under the broadside stretcher, 40% on top of it, while 60% in the middle of it. Such distribution of oxygen is not good for safety of cabin and could not meet the effect of hyperbaric oxygen therapy. The main reason of asymmetrical distribution of oxygen is that broadside stretcher exerts negative influence on oxygen diffusion and short-circuit at the bottom of the broadside stretcher (mal-design of input/output location of oxygen leads to automatic washing beneath the broadside stretcher). Whereas, the structure mentioned above is the most widely used one in the single pure oxygen chamber. If the entrance of oxygen is designed on top of or in the middle side of cabin ( on top of the broadside stretcher) may make oxygen concentration achieve up to 65~75% when the pressure is stable at 0.1MPa, thus, oxygen distribution will be symmetrical in cabin. Output of oxygen has nothing to do with oxygen concentration in cabin. However, broadside stretcher not only plays an important role in oxygen diffusion in cabin, but also divides the cabin into two parts. Therefore, to minimize the broadside stretcher plays a positive role in oxygen diffusion in cabin. Best way of washing the cabin- Oxygen concentration and distribution in cabin is the same with ordinary compression procedure that five minutes continued washing method or pressure achieved at 0.1 MPa directly.( with the cabin washed, oxygen concentration is higher by 2% on top of it, while the middle and the bottom part are higher by 4% versus not to wash the cabin.) However, washing method of normal pressure of the gap of the door is the most ideal way because it may elevate oxygen concentration up to 80% in 5 minutes inside the cabin. Selection of sampling outlet of oxygen-Sampling outlet of oxygen is located on top of the broadside stretcher in the middle of cabin at present. Oxygen concentration is affected by the following factors: differences of the width and height of broadside stretcher; irrationality of oxygen input/output design; different ways of washing. Hence, ordinary method for oxygen detection fails to reflect oxygen distribution in cabin. Solution for symmetric oxygen concentration is to change oxygen input location and adopt the most ideal way of washing. The specific way is to have the sampling outlet located on top of the broadside stretcher. Oxygen diffusion in cabin-In our 15 times experiment, we found no alteration of oxygen concentration all over the cabin with the pressure up to 0.1 MPa for 40 minutes. It is suggested that oxygen diffusion didn’t change apparently in both quantity and quality with the pressure up to 0.1 MPa for 40 minutes. Nevertheless, oxygen diffused obviously when pressure decreased. Oxygen concentration on the top and the middle of the cabin was almost the same whereas relatively higher at the bottom of cabin when there existed a broadside stretcher with the pressure of 0.06 MPa inside the cabin. Oxygen concentration is almost the same in the upper, middle and lower location when the pressure is about 0.03 MPa.

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