Microsatellite Development via Next-Generation Sequencing in Acacia stenophylla (Fabaceae) and Duma florulenta (Polygonaceae): Two Ecologically Important Plant Species of Australian Dryland Floodplains Bruce F. Murray1, Michael A. Reid1 and Shu-Biao Wu2 1Geography and Planning, School of Humanities Arts and Social Sciences, University of New England, Armidale, NSW, 2351, Australia. 2School of Environmental and Rural Science, University of New England, Armidale, NSW, 2351, Australia. Corresponding Author: Bruce Murray Email address:
[email protected] Postal address: Earth Studies Building C02, Geography and Planning, University of New England, Armidale, NSW, 2351, Australia. 1 PeerJ Preprints | https://doi.org/10.7287/peerj.preprints.27027v1 | CC BY 4.0 Open Access | rec: 10 Jul 2018, publ: 10 Jul 2018 1 Microsatellite Development via Next-Generation Sequencing 2 in Acacia stenophylla (Fabaceae) and Duma florulenta 3 (Polygonaceae): Two Ecologically Important Plant Species of 4 Australian Dryland Floodplains 5 6 Abstract 7 8 Duma florulenta and Acacia stenophylla are two ecologically important but 9 understudied species that naturally occur on the floodplains and riverbanks of Australia’s 10 arid and semi-arid river systems. This paper describes the discovery and characterization 11 of 12 and 13 polymorphic microsatellite markers for D. florulenta and A. stenophylla 12 respectively. The number of alleles per locus for D. florulenta ranged from 2-12 with an 13 average of 6.1. Across all samples, observed and expected heterozygosities ranged from 14 0.026 to 0.784 and 0.026 to 0.824, respectively and mean polymorphic information 15 content was equal to 0.453. For A. stenophylla, the number of alleles per locus ranged 16 between 2 and 8 with an overall mean of 4.8.