Evolution in Many-Sheeted Space-Time Contents

Total Page:16

File Type:pdf, Size:1020Kb

Evolution in Many-Sheeted Space-Time Contents CONTENTS 1 Evolution in Many-Sheeted Space-Time M. Pitk¨anen, June 19, 2019 Email: [email protected]. http://tgdtheory.com/public_html/. Recent postal address: Rinnekatu 2-4 A 8, 03620, Karkkila, Finland. Contents 1 Introduction 5 1.1 Questions And Answers About Evolution . .6 1.2 Topics Of The Chapter . .9 2 What Is Known About Pre-Biotic Evolution? 11 2.1 Some Believed-To-Be Facts About The Early History Of Life . 11 2.2 Standard Approaches Are Mechanistic . 11 2.3 The Notion Of Primordial Ocean . 12 2.4 Urey-Miller Experiment . 12 2.5 RNA World . 13 2.6 How Biochemical Pathways And DNA-Amino-Acid Code Emerged? . 13 2.7 Problems With The Polymerization In Primordial Ocean . 14 2.8 The Notion Of Protocell . 15 3 TGD Based Scenario About Pre-Biotic Evolution 15 3.1 Basic Prerequisites . 16 3.2 TGD Based Vision About Pre-Biotic Evolution . 16 3.2.1 Is life really a result of accident? . 17 3.2.2 The notions of magnetic body and plasmoid . 18 3.2.3 Does the Earth's magnetic field have a dark counterpart? . 18 3.2.4 Emergence of symbols at molecular level and new view about hydrogen bond, water, and bio-catalysts . 19 3.2.5 Universal metabolic currencies . 20 3.2.6 Time mirror mechanism, intentional action, memory, and remote metabolism 20 3.2.7 Emergence of membrane bounded structures . 20 3.2.8 Did life evolve in Mother Gaia's womb? . 21 3.2.9 Model for the genetic code . 22 CONTENTS 2 3.2.10 What makes possible the coherence of bio-chemical activities? . 22 3.3 Pre-Biotic Chemistry And New Physics . 22 3.3.1 Overall view . 23 3.3.2 Dark matter and the emergence of symbolic representations at molecular level 23 3.3.3 Evolution of pre-biotic chemistry as a sequence of bifurcations . 23 3.3.4 What selected the bio-molecules? . 25 3.3.5 Polymerization, dehydration, phosphorylation, and new physics . 25 3.3.6 Why DNA is stable inside cell nucleus? . 29 3.4 Could High Energy Phosphate Bond Be Negentropic Bond With Negative Binding Energy? . 29 3.4.1 Basic ideas . 30 3.4.2 General formulation of the model . 30 3.4.3 Quantitative estimates . 32 3.5 Water Memory And Braids . 33 3.5.1 Water memory: general considerations . 33 3.5.2 Water memory in terms of molecular braidings . 35 3.5.3 Why experimenter had to know which bottle contained the treated water? . 35 3.6 How Bio-Polymers Were Associated With Their Dark Counterparts? . 36 3.7 Two steps towards understanding of the origins of life . 38 3.7.1 The first step: binding of dark protons to formamido-pyrimidine . 38 3.7.2 Second step: Could shock waves due to extraterrestrial impacts have pro- duced RNA bases? . 40 3.8 Could the replication of mirror DNA teach something about chiral selection? . 41 3.8.1 Dark matter and chirality selection . 42 3.8.2 Model for the replication of DNA . 43 3.8.3 Differences between standard model and TGD based description . 46 4 Model For The Hierarchy Of Josephson Junctions 46 4.1 The Most Recent Model For The Generation Of Nerve Pulse . 47 4.2 Quantum Model For Sensory Receptor . 48 4.3 The Role Of Josephson Currents . 49 4.4 What Is The Role Of The Magnetic Body? . 50 4.4.1 Is magnetic body really needed? . 50 4.4.2 Is motor action time reversal of sensory perception in zero energy ontology? 51 4.4.3 What magnetic body looks like? . 51 4.4.4 What are the roles of Josephson and cyclotron photons? . 53 4.5 Dark Matter Hierarchies Of Josephson Junctions . 54 4.5.1 Maximization of Planck constant in quantum control and communication in living matter . 54 4.5.2 Dark hierarchy of Josephson junctions with a constant thickness . 55 4.5.3 An objection against a fractal hierarchy of Josephson junctions with thickness scaling as ~ .................................... 55 4.6 P-Adic Fractal Hierarchy Of Josephson Junctions . 56 4.6.1 The possibility of a p-adic hierarchy of membrane like structures accompa- nied by Josephson junctions . 56 4.6.2 Fractal hierarchy of magnetic bodies assignable to cell . 56 5 Physical Model For Genetic Code And Its Evolution 57 5.1 RNA World . 58 5.2 Programming Of Bio-Molecular Self Assembly Pathways From TGD Point Of View 58 5.2.1 Key ideas . 59 5.2.2 TGD view about the situation . 59 5.3 The Archeology Of TRNA Molecules As A Guideline . 60 5.3.1 The structure of the tRNA molecule . 60 5.3.2 Wobble base pairing . 61 5.3.3 Anomalous em charge and color singletness hypothesis for tRNA . 62 CONTENTS 3 5.3.4 The fossilized components of tRNA as record about the evolution of the recent form of the genetic code . 63 5.3.5 The components of tRNA as ribozymes which have acted originally as RNA polymerases . 64 5.3.6 From RNA world to RNA-tRNA world to RNA-DNA-tRNA world to DNA- RNA-protein world: how it went? . 65 5.4 Recent Genetic Code As A Fusion Of Singlet And Doublet Codes? . 68 5.4.1 RNA era and the transition to RNA-amino-acid era . 69 5.4.2 Symbiosis with membrane bounded structures . 70 5.4.3 Reverse transcription of RNA to DNA . 70 5.4.4 What were the first self replicators? . 71 5.5 Is RNA Era Continuing Inside Cell Nuclei? . 71 5.6 Could Nanno-Bacteria Correspond To Predecessors Of TheTriplet Life-Forms? . 72 5.6.1 Basic facts about nanno-bacteria . 72 5.6.2 Nanno-bacteria as RNA life? . 73 5.6.3 Was the encounter of nanno-bacteria and plasmoids the moment of Gaian fertilization? . 74 6 Did Life Evolve In The Womb Of Mother Gaia? 74 6.1 Quantum Version Of Expanding Earth Theory And Cambrian Explosion . 75 6.1.1 The claims of Adams . 76 6.1.2 The critic of Adams of the subduction mechanism . 76 6.1.3 Expanding Earth theories are not new . 77 6.1.4 Summary of TGD based theory of Expanding Earth . 77 6.1.5 Did intra-terrestrial life burst to the surface of Earth during Cambrian ex- pansion? . 79 6.2 Did Pre-Biotic Life Evolve In Mantle-Core Boundary? . 80 6.2.1 Some arguments supporting IT life . 80 6.2.2 Structure of the Earth's interior and IT life . 81 6.3 What Conditions Can One Pose On Life At Mantle-Core Boundary? . 82 6.3.1 Plasmoid life as minimum option . 82 6.3.2 Could topological quantum computation like activities be considered? . 83 6.3.3 Do metabolism and photosynthesis possess signatures telling about intra- terrestrial evolution? . 84 6.4 What About Analogs Of EEG? . 85 6.4.1 Conditions from the thermal stability of the analog of EEG . 86 6.4.2 What could the analog of EEG for IT look like? . 87 7 Comparison Of Mcfadden's Views With TGD 89 7.1 General Ideas . 89 7.1.1 McFadden's view about biochemistry . 90 7.1.2 Important problems of quantum biology . 91 7.2 Enzyme Action . 91 7.3 Quantum Evolution . 92 7.3.1 How evolution can create complexity? . 92 7.3.2 Criticism of RNA world . 92 7.3.3 Evolution of metabolism . 93 7.3.4 Quantum mechanism of mutations . 95 8 Great Vision About Biological Evolution And Evolution Of Brain 97 8.1 Basic Assumptions . 97 8.1.1 How to identify the preferred values of Planck constant? . 98 8.1.2 Tables about predicted time and length scales . 99 8.1.3 Electron and u quark are different . 99 8.2 Dark Matter Hierarchy And Big Leaps In Evolution . 100 8.2.1 A sketch about basic steps in evolution . 104 8.2.2 Division of the evolution to that of biological body and magnetic body . 106 CONTENTS 4 8.2.3 Biological evolution . 107 8.2.4 The evolution of magnetic body . 108 9 Oil Droplets In Water Solution As A Primitive Life Form? 110 9.1 Intelligent Oil Droplets . 111 9.2 Some Key Ideas Of TGD Inspired Quantum Biology . 112 9.3 General Ideas About Oil Droplets As A Primitive Life Form . ..
Recommended publications
  • OUR PLACE in the UNIVERSE DR CHARLES Lineweaver
    • I. WHERE ARE WE? Fig. 5b Earth from Moon with astronaut in foreground. In a canoe called the Earth, we are drifting down an unknown river. We Courtesy NASA (68·H·1401 and ASll-40-5903) What lies around the next bend? Some of us hear an ominous roar ahead - a water~ Some of us see the stream broadening and an immense unknown ocean on the horizon. We are on a journey through time. We may encounter other life forms or there may be j OrCharles Lineweaver is an billions of lifeless shores. We paddle and stab at the water trying to avoid jutting Australian Research Fellow in the School of Physics at the University of New South Wales. boulders and eddies. Many of us are bailing. We bail for about 80 years, then we give He studied undergraduate physics at Ludwig Maximillian Universitat, Germany and at our scoops and paddles to our children. If our canoe holds - if we can see far enough Kyoto University, Japan. He obtained his PhD from the University of California at ahead to avoid waterfalls and whirlpools, then surely our children's children's children... Berkeley for research on COBE satellite observations of the cosmic microwave background. will live to see this river empty into a placid infinite ocean. In the dark of the night, we After a postdoctoral fellowship in Strasbourg, France, he came gaze at the heavens and wonder who we are. How did we get on this canoe? to UNSWin 1997. He speaks four languages and has played semi· professional soccer.
    [Show full text]
  • Nanobacteria: an Alternative Mechanism for Pathogenic Intra- and Extracellular Calcification and Stone Formation
    Proc. Natl. Acad. Sci. USA Vol. 95, pp. 8274–8279, July 1998 Medical Sciences Nanobacteria: An alternative mechanism for pathogenic intra- and extracellular calcification and stone formation E. OLAVI KAJANDER* AND NEVA C¸ IFTC¸IOGLU Department of Biochemistry and Biotechnology, University of Kuopio, P.O.B. 1627, Fin-70211, Kuopio, Finland Communicated by J. Edwin Seegmiller, University of California, La Jolla, CA, April 23, 1998 (received for review January 9, 1998) ABSTRACT Calcium phosphate is deposited in many aminoglycoside antibiotics prevented their multiplication. Ac- diseases, but formation mechanisms remain speculative. cording to the 16S rRNA gene sequence (EMBL X98418 and Nanobacteria are the smallest cell-walled bacteria, only re- X98419), nanobacteria fall within the a-2 subgroup of Pro- cently discovered in human and cow blood and commercial cell teobacteria, which also includes Brucella and Bartonella species culture serum. In this study, we identified with energy- (10). The latter genera include human and animal pathogens dispersive x-ray microanalysis and chemical analysis that all that share similarities with nanobacteria, e.g., some antigens growth phases of nanobacteria produce biogenic apatite on (our unpublished data), intra- and extracellular location in the their cell envelope. Fourier transform IR spectroscopy re- host, and cytopathic effects (5). vealed the mineral as carbonate apatite. The biomineraliza- In this study, we provide evidence that nanobacteria can act as tion in cell culture media resulted in biofilms and mineral crystallization centers (nidi) for the formation of biogenic apatite aggregates closely resembling those found in tissue calcifica- structures. The mineralization process was studied in vitro with tion and kidney stones.
    [Show full text]
  • Light in and Sound Out: Emerging Translational Strategies for Photoacoustic Imaging
    Published OnlineFirst February 10, 2014; DOI: 10.1158/0008-5472.CAN-13-2387 Cancer Review Research Light In and Sound Out: Emerging Translational Strategies for Photoacoustic Imaging S. Zackrisson1,2,3,4,5, S.M.W.Y. van de Ven1,2,3,4, and S.S. Gambhir1,2,3,4 Abstract Photoacoustic imaging (PAI) has the potential for real-time molecular imaging at high resolution and deep inside the tissue, using nonionizing radiation and not necessarily depending on exogenous imaging agents, making this technique very promising for a range of clinical applications. The fact that PAI systems can be made portable and compatible with existing imaging technologies favors clinical translation even more. The breadth of clinical applications in which photoacoustics could play a valuable role include: noninvasive imaging of the breast, sentinel lymph nodes, skin, thyroid, eye, prostate (transrectal), and ovaries (transvaginal); minimally invasive endoscopic imaging of gastrointestinal tract, bladder, and circulating tumor cells (in vivo flow cytometry); and intraoperative imaging for assessment of tumor margins and (lymph node) metastases. In this review, we describe the basics of PAI and its recent advances in biomedical research, followed by a discussion of strategies for clinical translation of the technique. Cancer Res; 74(4); 979–1004. Ó2014 AACR. Introduction In this review, we will first describe the basics of PAI, Photoacoustic imaging (PAI), also referred to as optoacous- followed by a comprehensive overview of its clinical applica- tic imaging, is an emerging new imaging technique with tions. We will then discuss strategies for future translation to significant promise for biomedical applications. The key clinical practice.
    [Show full text]
  • Deliverable D.8.3 Literature Review of On-Line Bioprocess Monitoring
    NANOBE SEVENTH FRAMEWORK PROGRAMME THEME 2 Food, Agriculture and Fisheries, and Biotechnology Grant Agreement no: 227243 Project acronym: NANOBE Project title: Nano- and microtechnology –based analytical devices for online measurements of bioprocesses Funding Scheme: CA Deliverable D.8.3 Literature review of on-line bioprocess monitoring Report Due date of delivery: Month 18 Actual date of delivery: November 26th 2010 Responsible authors: Juha-Pekka Pitkänen (VTT), Heidi Turkia (VTT) Co-operative authors: Heli Sirén (VTT), Anna Rissanen (VTT), Guillaume Mernier (EPFL), Frédéric Reymond (DiagnoSwiss), Karen Lemke (iba), Francois Brunelle (CNRS-IEMN), Päivi Heimala (VTT) Project co-funded by the European Community's Seventh Framework Programme FP7 (2007-2013) Dissemination level PU Public PU PP Restricted to other programme participants (including the Commission Services) Restricted to a group specified by the consortium (including the Commission RE Services) Confidential, only for members of the consortium (including the Commission CO Services) Deliverable D.8.3 NANOBE GA no. 227243 TABLE OF CONTENTS TABLE OF CONTENTS 2 1 SUMMARY 3 2 PURPOSE AND SCOPE 3 3 INTRODUCTION 3 4 MEASUREMENT APPROACHES 8 4.1 Optical measurements 8 4.1.1 Optical single point measurements 8 4.1.2 Imaging measurements 9 4.2 Electrical measurements 10 4.3 Measurements based on chromatography-like separation 12 4.3.1 On-line capillary electrophoresis 12 4.3.2 On-line liquid chromatography 12 4.3.3 On-line gas chromatography 14 4.4 Direct mass spectrometric measurements 14 4.5 Measurements where biological component aids in recognition 15 5 SAMPLING AND SAMPLE TREATMENT APPROACHES 15 5.1 Automated sampling 16 5.2 Sample fractionation 18 5.3 Cell lysis 18 6 CONCLUSIONS 19 7 REFERENCES 19 2/25 Deliverable D.8.3 NANOBE GA no.
    [Show full text]
  • Allies and Enemies: How the World Depends on Bacteria
    Allies and Enemies This page intentionally left blank Allies and Enemies How the World Depends on Bacteria Anne Maczulak Vice President, Publisher: Tim Moore Associate Publisher and Director of Marketing: Amy Neidlinger Acquisitions Editor: Kirk Jensen Editorial Assistant: Pamela Boland Operations Manager: Gina Kanouse Senior Marketing Manager: Julie Phifer Publicity Manager: Laura Czaja Assistant Marketing Manager: Megan Colvin Cover Designer: Alan Clements Managing Editor: Kristy Hart Senior Project Editor: Lori Lyons Copy Editor: Geneil Breeze Proofreader: Apostrophe Editing Services Senior Indexer: Cheryl Lenser Compositor: Nonie Ratcliff Senior Manufacturing Buyer: Dan Uhrig © 2011 by Pearson Education, Inc. Publishing as FT Press Upper Saddle River, New Jersey 07458 FT Press offers excellent discounts on this book when ordered in quantity for bulk purchases or special sales. For more information, please contact U.S. Corporate and Government Sales, 1-800-382-3419, [email protected]. For sales outside the U.S., please contact International Sales at [email protected]. Company and product names mentioned herein are the trademarks or registered trademarks of their respective owners. All rights reserved. No part of this book may be reproduced, in any form or by any means, without permission in writing from the publisher. Printed in the United States of America First Printing July 2010 ISBN-10: 0-13-701546-1 ISBN-13: 978-0-13-701546-7 Pearson Education LTD. Pearson Education Australia PTY, Limited. Pearson Education Singapore, Pte. Ltd. Pearson Education North Asia, Ltd. Pearson Education Canada, Ltd. Pearson Educación de Mexico, S.A. de C.V. Pearson Education—Japan Pearson Education Malaysia, Pte. Ltd.
    [Show full text]
  • Size Limits of Very Small Microorganisms
    Size Limits of Very Small Microorganisms Proceedings of a Workshop Steering Group for the Workshop on Size Limits of Very Small Microorganisms Space Studies Board Commission on Physical Sciences, Mathematics, and Applications National Research Council NATIONAL ACADEMY PRESS Washington, D.C. NOTICE: The project that is the subject of this report was approved by the Governing Board of the National Research Council, whose members are drawn from the councils of the National Academy of Sciences, the National Academy of Engineering, and the Institute of Medicine. The members of the steering group responsible for the report were chosen for their special competences and with regard for appropriate balance. The National Academy of Sciences is a private, nonprofit, self-perpetuating society of distinguished scholars engaged in scientific and engineering research, dedicated to the furtherance of science and technology and to their use for the general welfare. Upon the authority of the charter granted to it by the Congress in 1863, the Academy has a mandate that requires it to advise the federal government on scientific and technical matters. Dr. Bruce Alberts is president of the National Academy of Sciences. The National Academy of Engineering was established in 1964, under the charter of the National Academy of Sciences, as a parallel organization of outstanding engineers. It is autonomous in its administration and in the selection of its members, sharing with the National Academy of Sciences the responsibility for advising the federal government. The National Academy of Engineering also sponsors engineering programs aimed at meeting national needs, encourages education and research, and recognizes the superior achievements of engineers.
    [Show full text]
  • Essa, Wisam Hindawi Hoidy, Spectrophotometric Determination
    160 Nano Biomed. Eng., 2020, Vol. 12, Iss. 2 Nano Biomed Eng 2020, 12(2): 160-166. doi: 10.5101/nbe.v12i2.p160-166. Research Article Spectrophotometric Determination of Cobalt(II) and Lead(II) Using (1,5-Dimethyl-2-Phenyl-4-((2,3,4- Trihydroxy Phenyl) Diazenyl)-1H-Pyrazol-3(2H)-One) as Organic Reagent: Using It as Antimicrobial and Antioxidants Shaimaa Mohsen. Essa , Wisam Hindawi Hoidy Department of Chemistry, College of Education, University of Al-Qadisiyah, Al-Qadisiyah, Iraq. Corresponding author. E-mail: [email protected] Received: Mar. 27, 2020; Accepted: Apr. 17, 2020; Published: Jun. 3, 2020 Citation: Shaimaa Mohsen. Essa, Wisam Hindawi Hoidy, Spectrophotometric Determination of Cobalt(II) and Lead(II) Using (1,5-Dimethyl-2- Phenyl-4-((2,3,4-Trihydroxy Phenyl) Diazenyl)-1H-Pyrazol-3(2H)-One) as Organic Reagent: Using It as Antimicrobial and Antioxidants. Nano Biomed. Eng., 2020, 12(2): 160-166. DOI: 10.5101/nbe.v12i2.p160-166. Abstract The azo organic reagent (1,5-dimethyl-2-phenyl-4-((2,3,4-trihydroxy phenyl) diazenyl)-1H-pyrazol- 3(2H)-one) (DPTPD) was prepared and used for the spectrophotometric determination of cobalt(II) and Lead(II), by the selective and surfactant-sensitized method based on the ternary complexes formation of Co(II) and Pb(II). The reagent had absorption maximum at 381 nm, and reacted with 2+ Co to form a purple reddish complex with λmax = 430 nm at pH = 7.5, while it formed a red complex 2+ with Pb of λmax = 417 nm at pH= 6. Beer's law for the determination over the range of 1-25 ppm and 1-33 ppm for Co(II) and Pb(II), respectively.
    [Show full text]
  • Nanobacterial System Towards Biofilm Forming Pseudomonas Oryzihabitans
    Nano Biomed. Eng., 2019, Vol. 11, Iss. 3 297 Nano Biomed Eng 2019, 11(3): 297-305. doi: 10.5101/nbe.v11i3.p297-305. Research Article Silver Nanoparticles as an Effective Anti- Nanobacterial System towards Biofilm Forming Pseudomonas oryzihabitans Shaimaa Obaid Hasson1 , Mohammed Jabber Al-Awady2, Mohanad Jawad Kadhim2, Hayder Shkhair Al-Janabi2 1Department of Microbiology, College of Veterinary, Al-Qasim Green University, Babylon, Iraq. 2Department of Genetic Engineering, Faculty of Biotechnology, Al Qasim Green University, Babylon, Iraq. Corresponding author. E-mail: [email protected] Received: Jun. 18, 2019; Accepted: Aug. 22, 2019; Published: Aug. 23, 2019. Citation: Shaimaa Obaid Hasson, Mohammed Jabber Al-Awady, Mohanad Jawad Kadhim, and Hayder Shkhair Al-Janabi, Silver Nanoparticles as an Effective Anti-Nanobacterial System towards Biofilm FormingPseudomonas oryzihabitans. Nano Biomed. Eng., 2019, 11(3): 297-305. DOI: 10.5101/nbe.v11i3.p297-305. Abstract Silver nanoparticles have been considered a powerful antimicrobial agents recently especially after increasing incidence of diseases associated with biofilm and multi-drug resistant pathogens required to find a novel path to eradicate that challenge. The present study aims to evaluate the antibacterial activity of biosynthesized silver nanoparticles (AgNPs) using a cell-free extract of Enterobacter cloacae and chemo synthesis by sodium borohydride (NaBH4) on biofilm-forming Pseudomonas oryzihabitans. Antimicrobial effect of silver nanoparticles in both types and in combination with imipenem were evaluated by agar well diffusion method. The results revealed a good response to inhibit biofilm-forming Pseudomonas oryzihabitans growth by silver nanoparticles antibacterial activity in both types (biological and chemical) and in combination with imipenem; the antimicrobial effect was increased and enhanced.
    [Show full text]
  • Emerging Translational Strategies for Photoacoustic Imaging
    Published OnlineFirst February 10, 2014; DOI: 10.1158/0008-5472.CAN-13-2387 Cancer Review Research Light In and Sound Out: Emerging Translational Strategies for Photoacoustic Imaging S. Zackrisson1,2,3,4,5, S.M.W.Y. van de Ven1,2,3,4, and S.S. Gambhir1,2,3,4 Abstract Photoacoustic imaging (PAI) has the potential for real-time molecular imaging at high resolution and deep inside the tissue, using nonionizing radiation and not necessarily depending on exogenous imaging agents, making this technique very promising for a range of clinical applications. The fact that PAI systems can be made portable and compatible with existing imaging technologies favors clinical translation even more. The breadth of clinical applications in which photoacoustics could play a valuable role include: noninvasive imaging of the breast, sentinel lymph nodes, skin, thyroid, eye, prostate (transrectal), and ovaries (transvaginal); minimally invasive endoscopic imaging of gastrointestinal tract, bladder, and circulating tumor cells (in vivo flow cytometry); and intraoperative imaging for assessment of tumor margins and (lymph node) metastases. In this review, we describe the basics of PAI and its recent advances in biomedical research, followed by a discussion of strategies for clinical translation of the technique. Cancer Res; 74(4); 1–26. Ó2014 AACR. Introduction In this review, we will first describe the basics of PAI, Photoacoustic imaging (PAI), also referred to as optoacous- followed by a comprehensive overview of its clinical applica- tic imaging, is an emerging new imaging technique with tions. We will then discuss strategies for future translation to significant promise for biomedical applications. The key clinical practice.
    [Show full text]
  • Ticle Adsorption
    C. Meng et al. nanobe.org OPEN ACCESS Article Nano Biomed Eng ISSN 2150-5578 http://nanobe.org Development of a Rapid and Convenient Method for Sampling Airborne Virus based on Nanopar- ticle Adsorption Chun Meng*, Yulin Xiong, Weiliang Zhuang, Hang Wang, Xian’ai Shi, Yanghao Guo Department of Bioengineering, College of Biological Science and Biotechnology, Fuzhou University, Fuzhou, Fujian 350108, China. *Corresponding authors. Email: [email protected], Tel: 86-591-22866379, Fax: 86-591-22866379 Abstract An improved aerobiological virus sampling method was developed based on adding adsorptive nanoparticles in samplers for concentrating viruses in sampling liquid buffers. The objectives of this research were to select effective adsorptive materials and optimize sampling parameters for increasing recovery of airborne viruses, such as influenza A virus or respiratory syndrome virus (RSV). Three kinds of polycation nanoparticles were evaluated for direct effects on absorption and desorption of influenza virus hemagglutinin and DNA. Chitosan particles showed good performance in absorption and desorption for both influenza virus hemagglutinin and DNA. A subsequent study evaluated the effects of collection buffer, pH and sampling time on the recovery of aerosolized viruses using a method for making direct comparisons of three treatments. The results demonstrated that various components in air-sampling collection buffer, impinger model, and sampling time, independently influenced the recovery of viruses. It was shown that adsorptive samplers with air disperser had the highest levels of sensitivity and repeatability in virus sampling. Both unspecifically adsorptive chitosan particles and specifically adsorptive particles labeled specific antibody to virus significantly enhanced recovery rate of aerosolized viruses. We succeeded to sample low level different pathogen viruses in outdoor environments with the optimized sampling system.
    [Show full text]
  • Immunoengineered Magnetic-Quantum Dot Nanobead
    Zhang et al. J Nanobiotechnol (2021) 19:116 https://doi.org/10.1186/s12951-021-00860-1 Journal of Nanobiotechnology RESEARCH Open Access Immunoengineered magnetic-quantum dot nanobead system for the isolation and detection of circulating tumor cells Pengfei Zhang1,2† , Mohamed S. Draz3,4,5†, Anwen Xiong6†, Wannian Yan2, Huanxing Han1,7* and Wansheng Chen1,8* Abstract Background: Highly efcient capture and detection of circulating tumor cells (CTCs) remain elusive mainly because of their extremely low concentration in patients’ peripheral blood. Methods: We present an approach for the simultaneous capturing, isolation, and detection of CTCs using an immuno-fuorescent magnetic nanobead system (iFMNS) coated with a monoclonal anti-EpCAM antibody. Results: The developed antibody nanobead system allows magnetic isolation and fuorescent-based quantifcation of CTCs. The expression of EpCAM on the surface of captured CTCs could be directly visualized without additional immune-fuorescent labeling. Our approach is shown to result in a 70–95% capture efciency of CTCs, and 95% of the captured cells remain viable. Using our approach, the isolated cells could be directly used for culture, reverse transcription-polymerase chain reaction (RT-PCR), and immunocytochemistry (ICC) identifcation. We applied iFMNS for testing CTCs in peripheral blood samples from a lung cancer patient. Conclusions: It is suggested that our iFMNS approach would be a promising tool for CTCs enrichment and detection in one step. Keywords: Magnetic nanoparticle, Quantum dots, Fluorescent magnetic nanobeads, Circulating tumor cells, Simultaneous capture and detection Introduction 5]. Compared with other early cancer diagnostic tools, Circulating tumor cells (CTCs) are free tumor cells shed such as genetic or metabolite omics analysis [6–8], cap- from original or metastatic tumors into the peripheral tured CTCs could provide more information for further blood [1–3].
    [Show full text]
  • Bio-Systems As Self-Organizing Quantum Systems
    1 BIO-SYSTEMS AS SELF-ORGANIZING QUANTUM SYSTEMS Matti Pitk¨anen K¨oydenpunojankatuD 11, 10900, Hanko, Finland iii Preface This book belongs to a series of online books summarizing the recent state Topological Geometro- dynamics (TGD) and its applications. TGD can be regarded as a unified theory of fundamental interactions but is not the kind of unified theory as so called GUTs constructed by graduate stu- dents at seventies and eighties using detailed recipes for how to reduce everything to group theory. Nowadays this activity has been completely computerized and it probably takes only a few hours to print out the predictions of this kind of unified theory as an article in the desired format. TGD is something different and I am not ashamed to confess that I have devoted the last 32 years of my life to this enterprise and am still unable to write The Rules. I got the basic idea of Topological Geometrodynamics (TGD) during autumn 1978, perhaps it was October. What I realized was that the representability of physical space-times as 4-dimensional surfaces of some higher-dimensional space-time obtained by replacing the points of Minkowski space with some very small compact internal space could resolve the conceptual difficulties of general rela- tivity related to the definition of the notion of energy. This belief was too optimistic and only with the advent of what I call zero energy ontology the understanding of the notion of Poincare invariance has become satisfactory. It soon became clear that the approach leads to a generalization of the notion of space-time with particles being represented by space-time surfaces with finite size so that TGD could be also seen as a generalization of the string model.
    [Show full text]