DOCSLIB.ORG

Assessing the Potential Impact of Sugarcane Varieties and Predation on Diatraea Saccharalis (F.) Populations in Southern Louisiana

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Assessing the Potential Impact of Sugarcane Varieties and Predation on Diatraea Saccharalis (F.) Populations in Southern Louisiana Louisiana State University LSU Digital Commons LSU Historical Dissertations and Theses Graduate School 1990 Assessing the Potential Impact of Sugarcane Varieties and Predation on Diatraea Saccharalis (F.) Populations in Southern Louisiana. Ricardo Tomas Bessin Louisiana State University and Agricultural & Mechanical College Follow this and additional works at: https://digitalcommons.lsu.edu/gradschool_disstheses Recommended Citation Bessin, Ricardo Tomas, "Assessing the Potential Impact of Sugarcane Varieties and Predation on Diatraea Saccharalis (F.) Populations in Southern Louisiana." (1990). LSU Historical Dissertations and Theses. 4896. https://digitalcommons.lsu.edu/gradschool_disstheses/4896 This Dissertation is brought to you for free and open access by the Graduate School at LSU Digital Commons. It has been accepted for inclusion in LSU Historical Dissertations and Theses by an authorized administrator of LSU Digital Commons. For more information, please contact [email protected]. INFORMATION TO USERS The most advanced technology has been used to photograph and reproduce this manuscript from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer. The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand corner and continuing from left to right in equal sections with small overlaps. Each original is also photographed in one exposure and is included in reduced form at the back of the book. Photographs included in the original manuscript have been reproduced xerographically in this copy. Higher quality 6" x 9" black and white photographic prints are available for any photographs or illustrations appearing in this copy for an additional charge. Contact UMI directly to order. University Microfilms International A Bell & Howell Information Company 300 North Zeeb Road, Ann Arbor, Ml 48106-1346 USA 313/761-4700 800/521-0600 Order Number 0104118 Assessing the potential impact of sugarcane varieties and predation onDiatraea saccharalis (F.) populations in southern Louisiana Bessin, Ricardo Tomds, Ph.D. The Louisiana State University and Agricultural and Mechanical Col., 1990 UMI 300 N. Zeeb Rd. Ann Aibor, MI 48106 r Assessing the Potential Impact of Sugarcane Varieties and Predation on Diatraea saccharalis (F.) Populations in Southern Louisiana. A Dissertation Submitted to the Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College in partial fulfillment of the requirements for the degree of Doctor of Philosophy in The Department of Entomology by Riccirdo Tomas Bessin M. Ap. Stat., Louisiana State University May 1990 Acknowledgments I wish to express my appreciation to Dr. Thomas E. Reagan who has served as my major advisor. My advisory committee consisting of Drs. E. Barry Moser, Freddie A. Martin, William H. White, and Elvis A. Heinrichs deserve special thanks for their continuing assistance in all aspects of my research program. Additionally, I would like to thank Billy W. Fuller and Jeff L. Flynn for their many days of assistance in collecting data, Dr. Ron Stinner for his assistance in developing the simulation model, and Mr. Pete Lannaux and Mr. Dennis Lannaux for the use of a portion of their plantation for the varietal evaluation studies. I would like to extend my sincere appreciation to all the people at the Department of Entomology, the LSU Sugar Station, the International Teaching and Research Foundation for Agricultural Sciences, and the Louisiana State University who have assisted me. I especially would like to thank my wife Susan and sons Scott and Sean for the many sacrifices they have made on my behalf. Table of Contents Page Acknowledgements .................................... il Table of Contents ................................... iii List of Tables ...................................... v List of Figures ..................................... viii Abstract ............................................ ix Introduction ........................................ 1 Literature Review ................................... 4 Chapter I Integration of Control Tactics for Management of the Sugarcane Borer (Lepidoptera: Pyralidae) in Louisiana Sugarcane ........................................... 12 Introduction ................................... 13 Materials and Methods ........................... 14 Results ........................................ 17 Discussion ..................................... 28 References Cited ............................... 37 Chapter II A Moth Production Index for Evaluating Sugarcane Cultivars for Resistance to the Sugarcane Borer (Lepidoptera: Pyralidae) ............................. 40 Introduction ................................... 41 Materials and Methods ...................... 43 Results ........................................ 45 Discussion ..................................... 50 References Cited ............................... 56 Table of Contents (continued) Page Chapter III Sugarcane Borer, Diatraea saccharalis (F.) (Lepidoptera:Pyralidae), Fecundity as Affected by Larval Development on Gramineous Host Plants ......... 59 Introduction ................................... 60 Materials and Methods ........................... 61 Results ........................................ 63 Discussion ..................................... 72 References Cited ............................... 75 Chapter IV Modeling Potential Impacts of Sugarcane Varieties and Predation on Sugarcane Borer (Lepidoptera: Pyralidae) Populations in Southern Louisiana ......... 78 Introduction ................................... 79 Materials and Methods ........................ 82 Results ........................................ 88 Discussion ..................................... 95 References Cited ............................... 99 Summary ............................................. 102 List of References ................................... 106 Appendices ................................. 116 A. Source code for the Diatraea saccharalis (F.) Stochastic Simulation model ...................... 117 B. Copyright permissions ........................... 133 Vita ................................................ 136 iv List of Tables Table Page Chapter I. Integration of Control Tactics for Management of the Sugarcane Borer (Lepidoptera: Pyralidae) in Louisiana Sugarcane 1. Effects of predator suppression on the abundance of selected insect and spider taxa in pitfall traps collected at 2-week intervals at the St. Gabriel Research Station during 1985, 1986 and 1987 .................................... 19 2. Comparison of Diatraea saccharalis damage and adult emergence from sugarcane as determined by end-of-season stalk inspection during 1985, 1986 and 1987 ............................... 21 3. Statistical analysis of management factor combinations in sugarcane on sugar yield, during 1985, 1986 and 1987 at the St. Gabriel Research Station, St. Gabriel, LA ..................... 27 4. Comparison of sugar yields among three sugarcane varieties during 1985, 1986 and 1987 with and without D. saccharalis insecticidal control at the St. Gabriel Research Station, St. Gabriel, LA .......................................... 29 5. Relative contributions of arthropod predation, varietal resistance, and fenvalerate in control­ ling sugarcane borer injury to sugarcane and adult moth production........................ 3 0 Chapter II. A Moth Production Index for Evaluating Sugarcane Cultivars for Resistance to the Sugarcane Borer (Lepidoptera: Pyralidae) 1. Comparison of Diatraea saccharalis damage to three commercial sugarcane cultivars and resultant survival of older larvae inside the stalks during a three-year study on the St. Gabriel Research Station ..................... 46 2. Damage by Diatraea saccharalis and relative survival inside the stalk in four commercial and 12 experimental cultivars (1985 LCP series) on the Glendale Plantation, near Edgard, La. 1987 ..... 47 v List of Tables (continued) Table Page 3. Comparison of Diatraea saccharalis adult emergence from three commercial sugarcane cultivars during a three-year study on the St. Gabriel Research Station ................... 51 Chapter III. Sugarcane Borer, Diatraea saccharalis (F.) (Lepidoptera: Pyralidae), Fecundity as Affected by Larval Development on Gramineous Host Plants Comparison of lengths, diameters, and weights of sugarcane borer pupae collected from four gramineous hosts at the St. Gabriel Research Station during 1987 and 1988 ............... 65 2 . Correlations among D. saccharalis fecundity and pupal physical characteristics collected from four gramineous hosts at the St. Gabriel Research Station during 1987 and 1988 ........ 68 3. Comparison of the mean fecundity and egg viability of D. saccharalis collected from four gramineous hosts at the St. Gabriel Research Station during 1987 and 1988 ................. 70 4. Reproductive statistics
Load more

Recommended publications
  • Interaction of Cultural, Biological, and Varietal Controls for Management of Stalk Borers in Louisiana Sugarcane
    insects Review Interaction of Cultural, Biological, and Varietal Controls for Management of Stalk Borers in Louisiana Sugarcane Thomas E. Reagan * and Megan M. Mulcahy * Department of Entomology, Louisiana State University AgCenter, Baton Rouge, LA 70803, USA * Correspondence: [email protected] (T.E.R.); [email protected] (M.M.M.) Received: 31 July 2019; Accepted: 11 September 2019; Published: 19 September 2019 Abstract: Diatraea saccharalis F is considered the most important pest of sugarcane in the United States. This article focuses on the history of pest management as it relates to the control of this stem borer in Louisiana sugarcane, and how control practices have become more in tune with integrated pest management paradigms. Various pest management strategies are employed against D. saccharalis and the interactions between each of these provide farmers with the tools needed to curb damaging infestations. However, the invasion of the Mexican rice borer, Eoreuma loftini (Dyar), and other confounding environmental factors have presented farmers, consultants, and researchers with new pest management challenges. We address these challenges and provide an overview of ongoing developments, particularly in the Louisiana sugarcane pest management program. Keywords: sugarcane; Diatraea saccharalis; integrated pest management; Eoreuma loftini; Louisiana; stalk borer 1. Introduction The sugarcane borer, Diatraea saccharalis F (Lepidoptera: Crambidae), is native to the West Indies, as well as Central and South America. It was introduced into Louisiana in the 1850s due to the continued importation of sugarcane for production in the state [1]. Since its introduction, D. saccharalis has long been regarded as the most damaging insect pest on the crop.
    [Show full text]
  • Tropical Insect Chemical Ecology - Edi A
    TROPICAL BIOLOGY AND CONSERVATION MANAGEMENT – Vol.VII - Tropical Insect Chemical Ecology - Edi A. Malo TROPICAL INSECT CHEMICAL ECOLOGY Edi A. Malo Departamento de Entomología Tropical, El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km. 2.5, Tapachula, Chiapas, C.P. 30700. México. Keywords: Insects, Semiochemicals, Pheromones, Kairomones, Monitoring, Mass Trapping, Mating Disrupting. Contents 1. Introduction 2. Semiochemicals 2.1. Use of Semiochemicals 3. Pheromones 3.1. Lepidoptera Pheromones 3.2. Coleoptera Pheromones 3.3. Diptera Pheromones 3.4. Pheromones of Insects of Medical Importance 4. Kairomones 4.1. Coleoptera Kairomones 4.2. Diptera Kairomones 5. Synthesis 6. Concluding Remarks Acknowledgments Glossary Bibliography Biographical Sketch Summary In this chapter we describe the current state of tropical insect chemical ecology in Latin America with the aim of stimulating the use of this important tool for future generations of technicians and professionals workers in insect pest management. Sex pheromones of tropical insectsUNESCO that have been identified to– date EOLSS are mainly used for detection and population monitoring. Another strategy termed mating disruption, has been used in the control of the tomato pinworm, Keiferia lycopersicella, and the Guatemalan potato moth, Tecia solanivora. Research into other semiochemicals such as kairomones in tropical insects SAMPLErevealed evidence of their presence CHAPTERS in coleopterans. However, additional studies are necessary in order to confirm these laboratory results. In fruit flies, the isolation of potential attractants (kairomone) from Spondias mombin for Anastrepha obliqua was reported recently. The use of semiochemicals to control insect pests is advantageous in that it is safe for humans and the environment. The extensive use of these kinds of technologies could be very important in reducing the use of pesticides with the consequent reduction in the level of contamination caused by these products around the world.
    [Show full text]
  • Resistance of Sugarcane Cultivars to Diatraea Saccharalis
    Resistance of sugarcane cultivars to Diatraea saccharalis Leila Luci Dinardo‑Miranda(1), Ivan Antônio dos Anjos(1), Viviane Pereira da Costa(1) and Juliano Vilela Fracasso(1) (1)Instituto Agronômico, Centro de Cana‑de‑açúcar, Rodovia SP 333, Km 321, CEP 14001‑970 Ribeirão Preto, SP, Brazil. E‑mail: [email protected], [email protected], [email protected], [email protected] Abstract – The objective of this work was to evaluate the oviposition preference of Diatraea saccharalis and the effect of ten sugarcane cultivars on larval development. Oviposition preference was assessed under greenhouse conditions by three releases of couples of moths, with subsequent counting of egg masses and eggs per plant. In order to evaluate the effect of the cultivars on larval development, each plant was infected with about 150 eggs, and, 29 days later, the total number of internodes, number of bored internodes, number of life forms found, larval and pupal weight and length, and the width of larval head capsule were evaluated. The cultivars IACSP94-2101 and IACSP96-2042, the least preferred by D. saccharalis for oviposition, and IACSP94-2094, the most unfavorable for larvae entrance and development, show resistance to the pest. Index terms: Saccharum, antibiosis, antixenosis, sugarcane borer. Resistência de cultivares de cana‑de‑açúcar a Diatraea saccharalis Resumo – O objetivo deste trabalho foi avaliar a preferência de oviposição de Diatraea saccharalis e o efeito de dez cultivares de cana-de-açúcar no desenvolvimento larval. A preferência para oviposição foi avaliada em casa de vegetação, por meio de três liberações de casais da praga, com posterior contagem de posturas e de ovos em cada planta.
    [Show full text]
  • Downloaded from BOLD Or Requested from Other Authors
    www.nature.com/scientificreports OPEN Towards a global DNA barcode reference library for quarantine identifcations of lepidopteran Received: 28 November 2018 Accepted: 5 April 2019 stemborers, with an emphasis on Published: xx xx xxxx sugarcane pests Timothy R. C. Lee 1, Stacey J. Anderson2, Lucy T. T. Tran-Nguyen3, Nader Sallam4, Bruno P. Le Ru5,6, Desmond Conlong7,8, Kevin Powell 9, Andrew Ward10 & Andrew Mitchell1 Lepidopteran stemborers are among the most damaging agricultural pests worldwide, able to reduce crop yields by up to 40%. Sugarcane is the world’s most prolifc crop, and several stemborer species from the families Noctuidae, Tortricidae, Crambidae and Pyralidae attack sugarcane. Australia is currently free of the most damaging stemborers, but biosecurity eforts are hampered by the difculty in morphologically distinguishing stemborer species. Here we assess the utility of DNA barcoding in identifying stemborer pest species. We review the current state of the COI barcode sequence library for sugarcane stemborers, assembling a dataset of 1297 sequences from 64 species. Sequences were from specimens collected and identifed in this study, downloaded from BOLD or requested from other authors. We performed species delimitation analyses to assess species diversity and the efectiveness of barcoding in this group. Seven species exhibited <0.03 K2P interspecifc diversity, indicating that diagnostic barcoding will work well in most of the studied taxa. We identifed 24 instances of identifcation errors in the online database, which has hampered unambiguous stemborer identifcation using barcodes. Instances of very high within-species diversity indicate that nuclear markers (e.g. 18S, 28S) and additional morphological data (genitalia dissection of all lineages) are needed to confrm species boundaries.
    [Show full text]
  • ENV /JM /M on O(2016)27 Unclassified
    Unclassified ENV/JM/MONO(2016)27 Organisation de Coopération et de Développement Économiques Organisation for Economic Co-operation and Development 29-Jun-2016 ___________________________________________________________________________________________ _____________ English - Or. English ENVIRONMENT DIRECTORATE JOINT MEETING OF THE CHEMICALS COMMITTEE AND Unclassified ENV/JM/MONO(2016)27 THE WORKING PARTY ON CHEMICALS, PESTICIDES AND BIOTECHNOLOGY Cancels & replaces the same document of 29 June 2016 CONSENSUS DOCUMENT ON THE BIOLOGY OF SORGHUM (Sorghum bicolor (L.) Moench) Series on Harmonisation of Regulatory Oversight in Biotechnology No. 62 English JT03398806 Complete document available on OLIS in its original format - This document and any map included herein are without prejudice to the status of or sovereignty over any territory, to the delimitation of Or. English international frontiers and boundaries and to the name of any territory, city or area. ENV/JM/MONO(2016)27 2 ENV/JM/MONO(2016)27 OECD Environment, Health and Safety Publications Series on Harmonisation of Regulatory Oversight in Biotechnology No. 62 Consensus Document on the Biology of Sorghum (Sorghum bicolor (L.) Moench) Environment Directorate Organisation for Economic Co-operation and Development Paris 2016 3 ENV/JM/MONO(2016)27 Also published in the Series on Harmonisation of Regulatory Oversight in Biotechnology: No. 1, Commercialisation of Agricultural Products Derived through Modern Biotechnology: Survey Results (1995) No. 2, Analysis of Information Elements Used in the Assessment of Certain Products of Modern Biotechnology (1995) No. 3, Report of the OECD Workshop on the Commercialisation of Agricultural Products Derived through Modern Biotechnology (1995) No. 4, Industrial Products of Modern Biotechnology Intended for Release to the Environment: The Proceedings of the Fribourg Workshop (1996) No.
    [Show full text]
  • BSES Limited
    BSES Limited REVIEW OF MOTH-BORER RESISTANCE SCREENING AND REPORT ON VISIT TO SASRI by Peter Samson SR09004 Contact: Peter Samson Principal Entomologist BSES Limited PMB 57 Mackay Mail Centre, Q 4741 Telephone: 07 4963 6815 Facsimile: 07 4954 5167 Email: [email protected] BSES is not a partner, joint venturer, employee or agent of SRDC and has no authority to legally bind SRDC, in any publication of substantive details or results of this Project. BSES Limited Publication Study Tour Report SR09004 November 2009 Copyright © 2009 by BSES Limited All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without the prior permission of BSES Limited. Warning: Our tests, inspections and recommendations should not be relied on without further, independent inquiries. They may not be accurate, complete or applicable for your particular needs for many reasons, including (for example) BSES Limited being unaware of other matters relevant to individual crops, the analysis of unrepresentative samples or the influence of environmental, managerial or other factors on production. Disclaimer: Except as required by law and only to the extent so required, none of BSES Limited, its directors, officers or agents makes any representation or warranty, express or implied, as to, or shall in any way be liable (including liability in negligence) directly or indirectly for any loss, damages, costs, expenses or reliance arising out of or in connection with, the accuracy, currency, completeness or balance of (or otherwise), or any errors in or omissions from, any test results, recommendations statements or other information provided to you.
    [Show full text]
  • In Diatraea Saccharalis (Lepidoptera: Crambidae) Pupae at Differe
    Reproduction of Tetrastichus howardi (Hymenoptera: Eulophidae) in Diatraea saccharalis (Lepidoptera: Crambidae) Pupae at Different Temperatures Author(s): Kellen Favero, Fabricio Fagundes Pereira, Jorge Braz Torres, Harley Nonato De Oliveira, Samir Oliveira Kassab and José Cola Zanuncio Source: Florida Entomologist, 98(3):865-869. Published By: Florida Entomological Society https://doi.org/10.1653/024.098.0308 URL: http://www.bioone.org/doi/full/10.1653/024.098.0308 BioOne (www.bioone.org) is a nonprofit, online aggregation of core research in the biological, ecological, and environmental sciences. BioOne provides a sustainable online platform for over 170 journals and books published by nonprofit societies, associations, museums, institutions, and presses. Your use of this PDF, the BioOne Web site, and all posted and associated content indicates your acceptance of BioOne’s Terms of Use, available at www.bioone.org/page/terms_of_use. Usage of BioOne content is strictly limited to personal, educational, and non-commercial use. Commercial inquiries or rights and permissions requests should be directed to the individual publisher as copyright holder. BioOne sees sustainable scholarly publishing as an inherently collaborative enterprise connecting authors, nonprofit publishers, academic institutions, research libraries, and research funders in the common goal of maximizing access to critical research. Reproduction of Tetrastichus howardi (Hymenoptera: Eulophidae) in Diatraea saccharalis (Lepidoptera: Crambidae) pupae at different temperatures Kellen Favero1, Fabricio Fagundes Pereira1, Jorge Braz Torres2, Harley Nonato De Oliveira3, Samir Oliveira Kassab1*, and José Cola Zanuncio4 Abstract Temperature is a major abiotic factor affecting insects. The aim of this study was to evaluate the development Tetrastichusof howardi (Olliff) (Hyme- noptera: Eulophidae) on Diatraea saccharalis F., sensu Guenée (Lepidoptera: Crambidae) pupae at 6 constant temperatures (16, 19, 22, 25, 28, and 31 °C).
    [Show full text]
  • Malpighian Tubules in Larvae of Diatraea Saccharalis (Lepidoptera
    Advances in Entomology, 2014, 2, 202-210 Published Online October 2014 in SciRes. http://www.scirp.org/journal/ae http://dx.doi.org/10.4236/ae.2014.24029 Malpighian Tubules in Larvae of Diatraea saccharalis (Lepidoptera; Crambidae): A Morphological Comparison between Non-Parasitized and Parasitized by Cotesia flavipes (Hymenoptera; Braconidae) Gislei Maria Rigoni1, Helio Conte2 1Departamento de Ciências Biológicas, Universidade Estadual do Centro Oeste (UNICENTRO), Guarapuava, Brazil 2Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá (UEM), Maringá, Brazil Email: [email protected], [email protected] Received 9 September 2014; revised 11 October 2014; accepted 20 October 2014 Copyright © 2014 by authors and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/ Abstract In Diatraea saccharalis larvae, the Malpighian tubules are found along the digestive tube, extend- ing from the middle mesenteric region to the end of the posterior intestine, where they come in contact with the rectum to form the cryptonephridium. Scanning and transmission electron mi- croscopy of non-parasitized and parasitized larvae by Cotesia flavipes have indicated that the tu- bules consist of secretory and reabsorption cells. In parasitized larvae, the occurrence of hemo- cytes and teratocytes around the tubules is indicative of their role in immunological defense; how- ever, they were not observed in non-parasitized larvae. At day 9 of parasitism, the mitochon- dria-containing vacuoles and myelin-like figures show signs of degeneration. The results of this study have confirmed that C. flavipes manipulates the physiology and biochemistry of D.
    [Show full text]
  • Identification of Natural Enemy in Stem Borers Complex in Sugar Cane at Tendaho Sugar Factory, Ethiopia
    Int. J. Adv. Res. Biol. Sci. (2019). 6(1): 25-32 International Journal of Advanced Research in Biological Sciences ISSN: 2348-8069 www.ijarbs.com DOI: 10.22192/ijarbs Coden: IJARQG(USA) Volume 6, Issue 1 - 2019 Research Article DOI: http://dx.doi.org/10.22192/ijarbs.2019.06.01.003 Identification of Natural Enemy in Stem Borers Complex in Sugar cane at Tendaho Sugar Factory, Ethiopia. Kidane T/Michael1*, Bayeh Mulatu2 and Mulugeta Negeri3 1Ethiopian Sugar Corporation, Research and Development, P. O. Box 15, Wonji, Ethiopia 2Ethiopia Food and Agricultural Organization (FAO) Addis Ababa, Ethiopia 3Department of Plant science, Ambo University, Ambo P. O. Box 19, Ethiopia *Corresponding Author: [email protected] Abstract Field study was conducted in Tendaho sugarcane plantation to identify the natural enemy of stalk borers under field condition using four commercial sugarcane varieties during 2013/14 cropping season. From 98 surveyed fields, a total of two different cotesia species were identified. the two species of natural enemy of stalk borers: Cotesia flavies and Cotesia sesamiae were recorded at Tendaho. Among these species, Cotesia flavies were the most dominant species. Among the different factors considered variety and cuttings had a significant effect on parasitism of stalk borer. Parasitism of stalk borer increased in the season. Among the different cuttings, ratoon had a significant variation in stalk borer larvae parasitism. Moreover, among the varieties, NCO334 had showed significant variation in percent parasitism of stalk borer larvae as compared to the other varieties in the plantation. as well as, in terms of percent parasitism of stalk borer larvae in the varieties B52/298, NCO334 and N14 showed no significant variation.
    [Show full text]
  • RAPID MEANS of SCREENING for RESISTANCE to PESTS in a SUGARCANE PLANT BREEDING SELECTION PROGRAMME by Cindy Moon Submitted in Fu
    RAPID MEANS OF SCREENING FOR RESISTANCE TO PESTS IN A SUGARCANE PLANT BREEDING SELECTION PROGRAMME By Cindy Moon Submitted in fulfilment of the requirements for the degree of Master of Science in Plant Pathology School of Agricultural, Earth and Environmental Sciences College of Agriculture, Engineering and Science University of KwaZulu-Natal Pietermaritzburg, South Africa July, 2014 DISSERTATION SUMMARY Chilo partellus (Lepidoptera: Crambidae) and Chilo sacchariphagus (Lepidoptera: Crambidae) are two stem borers which pose a threat to the South African sugar industry at present. The reliable supply of good quality insects for host-plant resistant studies is vital. The techniques used at the South African Sugar Research Institute (SASRI) for establishing and maintaining C. partellus colonies were described because these insects are vital in host-plant resistance research. Sugarcane agro- ecosystems in KwaZulu-Natal were surveyed for C. partellus, and species confirmation took place using cytochrome oxidase I subunit barcoding. A neighbor- joining tree showing Chilo phylogeny supported the concept of using C. partellus as a surrogate insect for C. sacchariphagus for host-plant resistant screening studies in South Africa. Artificial diets were developed to optimize insect growth and reproduction and to meet or exceed the nutritional requirements of the target insect. Experiments were conducted to test different diets, with the incorporation of various ingredients, and the use of different inoculation and rearing methods. Vials that were inoculated with two neonate larvae each gave greater mean larval weights and larval survival percentages compared to the multicell trays and plastic jars. An improved artificial diet for rearing C. partellus was established incorporating non-fat milk powder (2.35% m/v) and whole egg powder (1.75% m/v).
    [Show full text]
  • Bionomics of the Sugarcane Borer, Diatraea Saccharalis (F.), in Sweet Sorghum, Sorghum Bicolor (L.) Moench
    Louisiana State University LSU Digital Commons LSU Historical Dissertations and Theses Graduate School 1987 Bionomics of the Sugarcane Borer, Diatraea Saccharalis (F.), in Sweet Sorghum, Sorghum Bicolor (L.) Moench. Billy Wayne Fuller Louisiana State University and Agricultural & Mechanical College Follow this and additional works at: https://digitalcommons.lsu.edu/gradschool_disstheses Recommended Citation Fuller, Billy Wayne, "Bionomics of the Sugarcane Borer, Diatraea Saccharalis (F.), in Sweet Sorghum, Sorghum Bicolor (L.) Moench." (1987). LSU Historical Dissertations and Theses. 4447. https://digitalcommons.lsu.edu/gradschool_disstheses/4447 This Dissertation is brought to you for free and open access by the Graduate School at LSU Digital Commons. It has been accepted for inclusion in LSU Historical Dissertations and Theses by an authorized administrator of LSU Digital Commons. For more information, please contact [email protected]. INFORMATION TO USERS The most advanced technology has been used to photo­ graph and reproduce this manuscript from the microfilm master. UMI films the original text directly from the copy submitted. Thus, some dissertation copies are in typewriter face, while others may be from a computer printer. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyrighted material had to be removed, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, charts) are re­ produced by sectioning the original, beginning at the upper left-hand comer and continuing from left to right in equal sections with small overlaps. Each oversize page is available as one exposure on a standard 35 mm slide or as a 17" x 23" black and white photographic print for an additional charge.
    [Show full text]
  • Redalyc.Population Genetics of the Sugarcane Borer Diatraea Saccharalis
    Acta Scientiarum. Agronomy ISSN: 1679-9275 [email protected] Universidade Estadual de Maringá Brasil Alves Lopes, Denise; Belizário Cantagalli, Liriana; Barateiro Stuchi, Ana Lúcia Paz; Aparecida Mangolin, Claudete; Ruvolo-Takasusuki, Maria Claudia Colla Population genetics of the sugarcane borer Diatraea saccharalis (Fabr.) (Lepidoptera: Crambidae) Acta Scientiarum. Agronomy, vol. 36, núm. 2, abril-junio, 2014, pp. 189-194 Universidade Estadual de Maringá Maringá, Brasil Available in: http://www.redalyc.org/articulo.oa?id=303030420007 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative Acta Scientiarum http://www.uem.br/acta ISSN printed: 1679-9275 ISSN on-line: 1807-8621 Doi: 10.4025/actasciagron.v36i2.16211 Population genetics of the sugarcane borer Diatraea saccharalis (Fabr.) (Lepidoptera: Crambidae) Denise Alves Lopes, Liriana Belizário Cantagalli, Ana Lúcia Paz Barateiro Stuchi, Claudete Aparecida Mangolin and Maria Claudia Colla Ruvolo-Takasusuki* Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, Av. Colombo, 5790, 87.020-900, Maringá, Paraná, Brazil. *Author for correspondence. E-mail: [email protected] ABSTRACT. Diatraea saccharalis is the principal pest of sugarcane in Brazil and is found throughout the sugarcane crop. Information about its population genetics is scarce, but population genetic analysis is of particular importance as a basis for a successful pest control program. Pest control requires a constant evaluation of genetic variability so that appropriate strategies can be employed. In this study, the structure of D.
    [Show full text]