DOCSLIB.ORG

Choanephora Wet Rot of Cucumber and Its Management

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Choanephora Wet Rot of Cucumber and Its Management Choanephora Wet Rot of Cucumber and Its Management [khjs dk dks;uhQksjk vknzZxyu ,oa bldk izca/ku Sangeeta Choudhary Thesis Master of Science in Agriculture (Plant Pathology ) 2015 Department of Plant Pathology S.K.N. COLLEGE OF AGRICULTURE, JOBNER-303 329 SRI KARAN NARENDRA AGRICULTURE UNIVERSITY, JOBNER Choanephora Wet Rot of Cucumber and Its Management [khjs dk dks;uhQksjk vknzZxyu ,oa bldk izca/ku Thesis Submitted to the Sri Karan Narendra Agriculture University, Jobner In partial fulfillment of the requirement for the degree of Master of Science In the Faculty of Agriculture (Plant Pathology) By Sangeeta Choudhary 2015 Sri Karan Narendra Agriculture University, Jobner S.K.N. College of Agriculture, Jobner CERTIFICATE-I Dated :_______2015 This is to certify that Miss Sangeeta Choudhary has successfully completed the comprehensive examination held on 22 April 2015 as required under the regulation for Master’s degree. (K.S. Shekhawat) Professor & HEAD Department of Plant Pathology S.K.N. College of Agriculture, Jobner Sri Karan Narendra Agriculture University, Jobner S.K.N. College of Agriculture, Jobner CERTIFICATE-II Dated :________2015 This is to certify that the thesis entitled “Choanephora wet rot of cucumber and its management” submitted for the degree of Master of Science in the subject of Plant Pathology embodies bonafide research work carried out by Miss Sangeeta Choudhary under my guidance and supervision and that no part of this thesis has been submitted for any other degree. The assistance and help received during the course of investigation have been fully acknowledged. The draft of the thesis was also approved by the advisory committee on ........................ (K.S. Shekhawat) Professor & HEAD (R.P. Ghasolia) Department of Plant Pathology Major Advisor S.K.N. College of Agriculture, Jobner (S.N. Sharma) DEAN S.K.N. College of Agriculture, Jobner Sri Karan Narendra Agriculture University, Jobner S.K.N. College of Agriculture, Jobner CERTIFICATE-III Dated :______2015 This is to certify that the thesis entitled “Choanephora wet rot of cucumber and its management” submitted by Miss Sangeeta Choudhary to Sri Karan Narendra Agriculture University, Jobner, in partial fulfillment of the requirements for the degree of Master of Science in the subject of Plant Pathology after recommendation by the external examiner, was defended by the candidate before the following members of the examination committee. The performance of the candidate in the oral examination on his thesis has been found satisfactory. We therefore, recommend that the thesis be approved. (R.P. Ghasolia) (S. Godika) Major Advisor Advisor (B.L. Jat) (K. Ram Krishna) Advisor Director Education Nominee (K.S. Shekhawat) Professor & HEAD (S.N. Sharma) Department of Plant Pathology Dean S.K.N. College of Agriculture, S.K.N. College of Agriculture, Jobner Jobner Approved DIRECTOR EDUCATION S.K.N. Agriculture University, Jobner Sri Karan Narendra Agriculture University, Jobner S.K.N. College of Agriculture, Jobner CERTIFICATE-IV Dated : _____ 2015 This is to certify that Miss Sangeeta Choudhary of the Department of Plant Pathology, S.K.N., College of Agriculture, Jobner has made all corrections/ modifications in the thesis entitled “Choanephora wet rot of cucumber and its management” which were suggested by the external examiner and the advisory committee in the oral examination held on ______2015. The final copies of the thesis duly bound and corrected were submitted on ______2015 and forwarded herewith for approval. (R.P. Ghasolia) Major Advisor (K.S. Shekhawat) PROFESSOR & HEAD Department of Plant Pathology S.K.N. College of Agriculture, Jobner (S.N.Sharma) DEAN S.K.N. College of Agriculture, Jobner APPROVED DIRECTOR EDUCATION SKNAU, Jobner ACKNOWLEDGEMENT In the ecstasy. I express my esteem and profound sense of gratitude to Dr. R. P. Ghasolia, major advisor, Asstt. Professor, Department of Plant Pathology, S.K.N. College of Agriculture, Jobner, for his valuable and inspiring guidance, constant encouragement and keen interest during the course of present investigation and preparation of this research report. I convey the feeling of gratitude to the members of my advisory committee Dr. S. Godika, Professor, Deptt. of Plant Pathology, Dr. B.L. Jat, Professor and Head, Deptt. of Entomology, Dr. K. Ram Krishna, Director Education Nominee for the help rendered during the course of investigation. I am also deep and heartful greatfulness to the Dean, S. K. N. College of Agriculture, Jobner and Dr. K. S. Shekhawat, Head and Professor, Deptt. of Plant Pathology for providing necessary facilities during the course of investigation. I am again ecstatic to express my inherent sense of gratitude to Dr. Mahaveer Singh, Dr. R.L. Mahala, Dr. Rekha Kumawat, Deptt. of Plant Pathology, Dr. O.P. Garhwal, Asstt Professor, Deptt. of Horticulture and all the staff members, S.K.N. College of Agriculture, Jobner whose cooperation made this investigation smooth and easy. Down the memory lane. I am very much thankful to my dear friends Manju, Sukhlal, Jitendra, Vishambar, Anita, Nagar, Sunita my seniors, Sanju di, Arjun Ji, sajjan Ji, suresh Ji, Manoj Ji and my lovely juniors Madhu, Pankaj, Bhagwati, Rajesh, Roshan, Raj kumar, Pramod, Suresh and Rajbala with whom I went hand in hand during my study and research work. My vocabulary falls short to express heartiest regards to my grandmother Smt. Radha Devi, father Sh. Raghunath Prasad Ji, lovely mother Smt. Prem Devi, Elder brother Narendra Mohan, younger brother Dinesh, Bhabhi Sumitra and lovely niece Divya and Nivedita whose consistent encouragement and blessing are beyond my expression that brought me here up to dream without which it could not have been sketched. I am also grateful to Sh. Suresh Yadav of Vimal Computers, Jobner, who typed this manuscript. Last but not the least, a millions of thanks to God the almighty who made me to complete this task and made every job a success for me. Place: Jobner Dated: (SANGEETA CHOUDHARY) CONTENTS CHAPTER PARTICULARS PAGE NO. NO. 1. INTRODUCTION ……….. 2. REVIEW OF LITERATURE ……….. 3. MATERIALS AND METHODS ……….. 4. RESULTS ……….. 5. DISCUSSION ……….. 6. SUMMARY ……….. LITERATURE CITED ……….. ABSTRACT (English) ……….. ABSTRACT (Hindi) ……….. LIST OF TABLES Table Particulars Page No. No. 4.1 Pathogenicity of Choanephora wet rot of cucumber ----------- 4.2 Effect of temperature on mycelial growth of ----------- Choanephora cucurbitarum 4.3 Effect of relative humidity on mycelial growth of ----------- Choanephora cucurbitarum at 25 + 10C 4.4 Effect of different media on mycelial growth of ----------- Choanephora cucurbitarum at 25 + 10C 4.5 Efficacy of salicylic acid on Choanephora wet rot of ----------- cucumber 4.6 Efficacy of fungicides on mycelial growth of ----------- Choanephora cucurbitarum at 25 + 10C (in vitro) 4.7 Efficacy of different fungicides against Choanephora wet ----------- rot of cucumber (in vivo) 4.8 Efficacy of bioagents against Choanephora wet rot of ----------- cucumber 4.9 Screening of cucumber varieties against Choanephora ----------- cucurbitarum LIST OF FIGURES Figure Particulars Page No. No. 4.1 Effect of temperature on mycelial growth of ……… Choanephora cucurbitarum 4.2 Effect of relative humidity on mycelial growth of ……… Choanephora cucurbitarum at 25 + 10C 4.3 Effect of different media on mycelial growth of ……… Choanephora cucurbitarum at 25 + 10C 4.4 Efficacy of salicylic acid on Choanephora wet rot of ……… cucumber 4.5 Efficacy of fungicides on mycelial growth of ……… Choanephora cucurbitarum at 25 + 10C in vitro 4.6 Efficacy of different fungicides against Choanephora ……… wet rot of cucumber in vivo 4.7 Efficacy of bioagents against Choanephora wet rot of ……… cucumber 4.8 Screening of cucumber varieties against Choanephora ……… cucurbitarum LIST OF PLATES Plate Particulars Page No. No. 4.1 Morphological characters of Choanephora cucurbitarum …… 4.2 Pathogenicity test ……. 4.3 Symptoms of Choanephora wet rot on cucumber plants …… 4.4 Effect of temperature on mycelial growth of Choanephora ……. cucurbitarum 4.5 Effect of relative humidity on mycelial growth of …… Choanephora cucurbitarum at 25 + 10C 4.6 Effect of different media on mycelial growth of ……. Choanephora cucurbitarum at 25 + 10C 4.7 Efficacy of fungicides on mycelial growth of …… 0 Choanephora cucurbitarum at 25 + 1 C (in vitro) 1. Introduction Cucumber (Cucumis sativus L.) is known as “Kheera” in Hindi. It is a popular and widely cultivated summer vegetable in India which belongs to family Cucurbitaceae. It is one of the important vegetable crops which supply edible product and fibre. Cucumber is used as salad or as vegetable and as desert fruit specially in Rajasthan. In India, cucumber is commonly grown in Andhra Pradesh, Karnataka, Assam and Rajasthan, generally towards the riversides. Rajasthan state provides the maximum potential for the production of cucumber because of its agro-climatic conditions that are best suited for their growth and yield. According to De Candole (1967) cucumber is an indigenous vegetable of India. Purseglove (1967) has suggested that all cultigens (Cucumis sativus) originated from northern India where the related Cucumis hardwicki Royle occurs as wild, although this might be a “weedy” form of Cucumis sativus, which has escaped from cultivation. Cucumber has been cultivated in India for at least three thousand years (Rai and Yadav, 2005). Cucumber is an annual deep-rooted crop with tendrils and hairy leaves. The plants may have an indeterminate, determinate or a compact
Load more

Recommended publications
  • Characterization of Two Undescribed Mucoralean Species with Specific
    Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 26 March 2018 doi:10.20944/preprints201803.0204.v1 1 Article 2 Characterization of Two Undescribed Mucoralean 3 Species with Specific Habitats in Korea 4 Seo Hee Lee, Thuong T. T. Nguyen and Hyang Burm Lee* 5 Division of Food Technology, Biotechnology and Agrochemistry, College of Agriculture and Life Sciences, 6 Chonnam National University, Gwangju 61186, Korea; [email protected] (S.H.L.); 7 [email protected] (T.T.T.N.) 8 * Correspondence: [email protected]; Tel.: +82-(0)62-530-2136 9 10 Abstract: The order Mucorales, the largest in number of species within the Mucoromycotina, 11 comprises typically fast-growing saprotrophic fungi. During a study of the fungal diversity of 12 undiscovered taxa in Korea, two mucoralean strains, CNUFC-GWD3-9 and CNUFC-EGF1-4, were 13 isolated from specific habitats including freshwater and fecal samples, respectively, in Korea. The 14 strains were analyzed both for morphology and phylogeny based on the internal transcribed 15 spacer (ITS) and large subunit (LSU) of 28S ribosomal DNA regions. On the basis of their 16 morphological characteristics and sequence analyses, isolates CNUFC-GWD3-9 and CNUFC- 17 EGF1-4 were confirmed to be Gilbertella persicaria and Pilobolus crystallinus, respectively.To the 18 best of our knowledge, there are no published literature records of these two genera in Korea. 19 Keywords: Gilbertella persicaria; Pilobolus crystallinus; mucoralean fungi; phylogeny; morphology; 20 undiscovered taxa 21 22 1. Introduction 23 Previously, taxa of the former phylum Zygomycota were distributed among the phylum 24 Glomeromycota and four subphyla incertae sedis, including Mucoromycotina, Kickxellomycotina, 25 Zoopagomycotina, and Entomophthoromycotina [1].
    [Show full text]
  • Molecular Phylogenetic and Scanning Electron Microscopical Analyses
    Acta Biologica Hungarica 59 (3), pp. 365–383 (2008) DOI: 10.1556/ABiol.59.2008.3.10 MOLECULAR PHYLOGENETIC AND SCANNING ELECTRON MICROSCOPICAL ANALYSES PLACES THE CHOANEPHORACEAE AND THE GILBERTELLACEAE IN A MONOPHYLETIC GROUP WITHIN THE MUCORALES (ZYGOMYCETES, FUNGI) KERSTIN VOIGT1* and L. OLSSON2 1 Institut für Mikrobiologie, Pilz-Referenz-Zentrum, Friedrich-Schiller-Universität Jena, Neugasse 24, D-07743 Jena, Germany 2 Institut für Spezielle Zoologie und Evolutionsbiologie, Friedrich-Schiller-Universität Jena, Erbertstr. 1, D-07743 Jena, Germany (Received: May 4, 2007; accepted: June 11, 2007) A multi-gene genealogy based on maximum parsimony and distance analyses of the exonic genes for actin (act) and translation elongation factor 1 alpha (tef ), the nuclear genes for the small (18S) and large (28S) subunit ribosomal RNA (comprising 807, 1092, 1863, 389 characters, respectively) of all 50 gen- era of the Mucorales (Zygomycetes) suggests that the Choanephoraceae is a monophyletic group. The monotypic Gilbertellaceae appears in close phylogenetic relatedness to the Choanephoraceae. The mono- phyly of the Choanephoraceae has moderate to strong support (bootstrap proportions 67% and 96% in distance and maximum parsimony analyses, respectively), whereas the monophyly of the Choanephoraceae-Gilbertellaceae clade is supported by high bootstrap values (100% and 98%). This suggests that the two families can be joined into one family, which leads to the elimination of the Gilbertellaceae as a separate family. In order to test this hypothesis single-locus neighbor-joining analy- ses were performed on nuclear genes of the 18S, 5.8S, 28S and internal transcribed spacer (ITS) 1 ribo- somal RNA and the translation elongation factor 1 alpha (tef ) and beta tubulin (βtub) nucleotide sequences.
    [Show full text]
  • Ohio Plant Disease Index
    Special Circular 128 December 1989 Ohio Plant Disease Index The Ohio State University Ohio Agricultural Research and Development Center Wooster, Ohio This page intentionally blank. Special Circular 128 December 1989 Ohio Plant Disease Index C. Wayne Ellett Department of Plant Pathology The Ohio State University Columbus, Ohio T · H · E OHIO ISJATE ! UNIVERSITY OARilL Kirklyn M. Kerr Director The Ohio State University Ohio Agricultural Research and Development Center Wooster, Ohio All publications of the Ohio Agricultural Research and Development Center are available to all potential dientele on a nondiscriminatory basis without regard to race, color, creed, religion, sexual orientation, national origin, sex, age, handicap, or Vietnam-era veteran status. 12-89-750 This page intentionally blank. Foreword The Ohio Plant Disease Index is the first step in develop­ Prof. Ellett has had considerable experience in the ing an authoritative and comprehensive compilation of plant diagnosis of Ohio plant diseases, and his scholarly approach diseases known to occur in the state of Ohia Prof. C. Wayne in preparing the index received the acclaim and support .of Ellett had worked diligently on the preparation of the first the plant pathology faculty at The Ohio State University. edition of the Ohio Plant Disease Index since his retirement This first edition stands as a remarkable ad substantial con­ as Professor Emeritus in 1981. The magnitude of the task tribution by Prof. Ellett. The index will serve us well as the is illustrated by the cataloguing of more than 3,600 entries complete reference for Ohio for many years to come. of recorded diseases on approximately 1,230 host or plant species in 124 families.
    [Show full text]
  • O Pap N Ayaa
    TTEECCHHNNIICCAALL DDOOCCUUMMEENNTT FFOORR MMAARRKKEETT AACCCCEESSSS OONN PPAAPPAAYYAA CROP PROTECTIOACKNOWLEDGEMEN & PLANT QUARANNTTI NE SERVICES DIVISION DEPARTMENT OF AGRICULTURE KUALA LUMPUR MALAYSIA 2004 Technical Document For Market Access On Papaya: 2004 i ACKNOWLEDGEMENT Ms. Asna Booty Othman, Director, Crop Protection and Plant Quarantine Services Division, Department of Agriculture Malaysia, wishes to extend her appreciation and gratitude to the following for their contribution, assistance and cooperation in the preparation of this Technical Document For Papaya:- Mr. Muhamad Hj. Omar, Assistant Director, Phytosanitary and Export Control Section, Crop Protection and Plant Quarantine Services Division, Department of Agriculture Malaysia; Ms. Nuraizah Hashim, Agriculture Officer, Phytosanitary and Export Control Section, Crop Protection and Plant Quarantine Services Division, Department of Agriculture Malaysia; Mr. Yusof Othman, Agriculture Officer, Insects Section, Crop Protection and Plant Quarantine Services Division, Department of Agriculture Malaysia; Mr. Arizal Arshad, Agriculture Officer, Enforcement Section, Crop Protection and Plant Quarantine Services Division, Department of Agriculture Malaysia; Mr. Mansor Mohammad, Assistant Agriculture Officer, Insects Section, Crop Protection and Plant Quarantine Services Division, Department of Agriculture Malaysia; Mr. Rushdan Talib, Agriculture Officer, Vertebrate and Mollusca Section, Crop Protection and Plant Quarantine Services Division, Department of Agriculture Malaysia; Ms.
    [Show full text]
  • Studies on Physiological Parameters of Choanephora Cucurbitarum, The
    Journal of Pharmacognosy and Phytochemistry 2018; 7(3): 3227-3229 E-ISSN: 2278-4136 P-ISSN: 2349-8234 JPP 2018; 7(3): 3227-3229 Studies on physiological parameters of Received: 05-03-2018 Accepted: 10-04-2018 Choanephora cucurbitarum, the incitant of wet rot of cucumber Choudhary Sangeeta Department of Plant Pathology, SKNCoA Jobner, Jaipur, Rajasthan, India Choudhary Sangeeta, Ghasolia RP and Lakhran Lalita Ghasolia RP Abstract Shri Karan Narendra Agriculture A newly documented disease, namely Choanephora wet rot of cucumber (Cucumis sativus L.) caused University Jobner, Jaipur, by Choanephora cucurbitarum was found at SKN COA, Jobner, in Zayad 2014. The colonies of the Rajasthan, India causal fungus grown on potato dextrose agar at 25°C are white at the early stage of growth with abundant Lakhran Lalita aerial mycelium, but at later growth stages turn pale yellow. Sporangia and sporangiola are readily Shri Karan Narendra Agriculture observed on the tip of upright sporangiophores, which are numerous on aerial mycelium. Effect of University Jobner, Jaipur, temperature, RH and media on mycelia growth of Choanephora cucurbitarum was studied in vitro. Rajasthan, India Maximum mycelia growth was found at 25 C and 30 C. 100% relative humidity favours the disease also. A significantly decrease in mycelia growth was observed at 70% relative humidity. Among five different media PDA was found superior. This was followed by martin medium. Mycelial growth was not recorded on Oat meal agar. Keywords: Choanephora cucurbitarum, choanephora wet rot Introduction Cucumber (Cucumis sativus L.) is known as “Kheera” in Hindi. It is a popular and widely cultivated summer vegetable in India which belongs to family Cucurbitaceae.
    [Show full text]
  • CAROTENOIDS in the FUNGI MUCORALES 5 This Mold the Saturated Polyenes Were Produced Much More Rapidly Than Ij-Carotene
    2 8 2 5 I~ Illp·8 2 5 1.0 :~ 11111 . 11111 . 1.0 I~ 1= 11111 . ~ II~-~ 2.2 I~ IIli?~ 2.2 Ui I" ~ I~ I; I~ w J:Z ~ 1,1,g ~ m,t"g 2.0 LiI. '" ... " 1.1 ...... "" 1.1 ...u,~u -- - --- 11111 1.25 1111,1.4 111111.6 111111.25 !lII,1.4 111111.6 MICROCOPY RESOLUTION TEST CHART MICROCOPY RESOLUTION TEST CHART NATIONAL BUREAU OF STANDARDS-J963-A NATIONAL BUREAU OF STANDARDS,1963-A ~AROTENOIDS in the FUNGI e­ :..~ CJ")coMUCORALES .~, ~ .',:.; , " Special Reference to Choanephoraceae U.S. DEPARTMENT OF AGRICULTURE AGRICULTURAL RESEARCH SERVICE CONTENTS Pllg" INTRODUCTION _________________________________________ _ CAROTENE IN ]\I[UCORALES_____________________ • ___ ••• ____ 2 FUNCTION OF CAROTENE IN SEXUALI'l'Y OF l\l{ucorur,J.JS_______ 7 CAUOTENE IN THE FAMILY CHOANEPHOHACEAE______________ 9 OeeulTcnce and History of the Chonncphora(,Nl(' __ _ _ _ _ _ _I0 Life Cycle_---- ________________________ ., _ _ _ _ _ _ _ _ _ _ _ _ 14 Cnrotene in Chonnephorneelle_________________________ 17 LITEHATURE CITED______________________________________ 23 Cllrotene in l\{ucornil',s______________________ .________ 2:~ Oc('ulTcnce llnd ]~ift· l 'yell'S of C'hoanephomeell(' ___ _ _ _ _ _ _ ao This review wus madc ns pIU'L of the irl\resLiglltions bl'ing eonducted at the Northern Hegionnl ]{csellJ'eh Laboratory on indust.rial uliliZtl­ tion of CCI'CIlI gmins. The ARS Oultmc Collection, mnintlline(\ there, is one of thc world's IUl'gest and most ('omplctc ('ollections of industrially important, uILCtcl'in" molds, Ildinornyectes, n,nd yCilStS. The Collection serves as n, sourcc of ItlIt,hcntic miCl'o-oJ'gnlli~ms for the fermentative production of orgillli<.~ neids, vitnmins, Illltibiotics, enzymes, feeds, bcvemgcs, Ilm\ foods.
    [Show full text]
  • Bibliography of the Genetic Resources of Traditional African Vegetables
    Neglected leafy green vegetable crops in Africa Vol. 2 Bibliographyof traditional ofAfrican the genetic vegetables resources N.M. Mnzava, J.A. Dearing, L. Guarino, J.A. Chweya (compilers) and H. de Koeijer (editor) Netherlands Ministry of Foreign Affairs Development Cooperation Bibliographyof traditional ofAfrican the genetic vegetables resources N.M. Mnzava, J.A. Dearing, L. Guarino, J.A. Chweya (compilers) and H. de Koeijer (editor) The International Plant Genetic Resources Institute (IPGRI) is an autonomous international scientific orga- nization, supported by the Consultative Group on International Agricultural Research (CGIAR). IPGRI’s mandate is to advance the conservation and use of genetic diversity for the well-being of present and future generations. IPGRI’s headquarters is based in Rome, Italy, with offices in another 14 countries worldwide. It operates through three programmes: (1) the Plant Genetic Resources Programme, (2) the CGIAR Genetic Resources Support Programme, and (3) the International Network for the Improvement of Banana and Plantain (INIBAP). CAB International (CABI) is an international, intergovernmental, not-for-profit organization. Its mission is to help improve human welfare worldwide through the dissemination, application and generation of scientific knowledge in support of sustainable development, with emphasis on agriculture, forestry, human health and the management of natural resources, and with particular attention to the needs of developing countries. The international status of IPGRI is conferred under
    [Show full text]
  • Twig Blight of Chilli Caused by Choanephora Cucurbitarum in Telangana
    Available online at www.ijpab.com Chandrakala and Vidyasagar Int. J. Pure App. Biosci. 6 (4): 521-526 (2018) ISSN: 2320 – 7051 DOI: http://dx.doi.org/10.18782/2320-7051.6902 ISSN: 2320 – 7051 Int. J. Pure App. Biosci. 6 (4): 521-526 (2018) Research Article Twig Blight of Chilli Caused by Choanephora cucurbitarum in Telangana J. Chandrakala* and B. Vidyasagar College of Agriculture, Professor Jaya Shankar Telangana State Agricultural University Rajendranagar, Hyderabad-500030, Telanagana, India *Corresponding Author E-mail: [email protected] Received: 6.07.2018 | Revised: 17.08.2018 | Accepted: 25.08.2018 ABSTRACT In December 2014 twig blight on chilli (Capsicum frutescens) caused by Choanephora cucurbitarum was observed during survey in farmer’s fields in major chilli growing areas of Telangana state. The symptoms of Choanephora twig blight of chilli were observed under natural field condition on leaves, stem, petioles and fruits during survey. Isolation was done from the plant parts and showed presence of Choanephora cucurbitarum which was identified on the basis of its morphological characters. Twig blight disease caused by the pathogen Choanephora cucurbitarum was identified based on morphological and cultural characters. The disease began with water-soaking and dark-green lesions, and then the infected tissues were rapidly rotten. The drooping multisporous sporangia were subglobose in shape and 90.15 - 100 μm in size. Sporangiospores were elliptic, fusiform or ovoid in shape, light brown or dark brown in color and are characterized by longitudinal striatures on the wall surface and 12-19 x 7.23- 9.5 μm by size. The sporangiophore (conidiophore) from which the monosporous sporangiola arose was long slender, branched at the apex with primary vesicle from which secondary vesicles were produced on the stalks which bears sporangiospores (conidia).
    [Show full text]
  • Dear Author, Here Are the Proofs of Your Article. • You Can Submit Your
    Dear Author, Here are the proofs of your article. • You can submit your corrections online, via e-mail or by fax. • For online submission please insert your corrections in the online correction form. Always indicate the line number to which the correction refers. • You can also insert your corrections in the proof PDF and email the annotated PDF. • For fax submission, please ensure that your corrections are clearly legible. Use a fine black pen and write the correction in the margin, not too close to the edge of the page. • Remember to note the journal title, article number, and your name when sending your response via e-mail or fax. • Check the metadata sheet to make sure that the header information, especially author names and the corresponding affiliations are correctly shown. • Check the questions that may have arisen during copy editing and insert your answers/ corrections. • Check that the text is complete and that all figures, tables and their legends are included. Also check the accuracy of special characters, equations, and electronic supplementary material if applicable. If necessary refer to the Edited manuscript. • The publication of inaccurate data such as dosages and units can have serious consequences. Please take particular care that all such details are correct. • Please do not make changes that involve only matters of style. We have generally introduced forms that follow the journal’s style. Substantial changes in content, e.g., new results, corrected values, title and authorship are not allowed without the approval of the responsible editor. In such a case, please contact the Editorial Office and return his/her consent together with the proof.
    [Show full text]
  • Genome Analysis of a Zygomycete Fungus Choanephora Cucurbitarum
    www.nature.com/scientificreports OPEN Genome Analysis of a Zygomycete Fungus Choanephora cucurbitarum Elucidates Necrotrophic Features Received: 23 August 2016 Accepted: 11 November 2016 Including Bacterial Genes Related Published: 16 January 2017 to Plant Colonization Byoungnam Min1, Ji-Hyun Park2, Hongjae Park1, Hyeon-Dong Shin2 & In-Geol Choi1 A zygomycete fungus, Choanephora cucurbitarum is a plant pathogen that causes blossom rot in cucurbits and other plants. Here we report the genome sequence of Choanephora cucurbitarum KUS- F28377 isolated from squash. The assembled genome has a size of 29.1 Mbp and 11,977 protein-coding genes. The genome analysis indicated that C. cucurbitarum may employ a plant pathogenic mechanism similar to that of bacterial plant pathogens. The genome contained 11 genes with a Streptomyces subtilisin inhibitor-like domain, which plays an important role in the defense against plant immunity. This domain has been found only in bacterial genomes. Carbohydrate active enzyme analysis detected 312 CAZymes in this genome where carbohydrate esterase family 6, rarely found in dikaryotic fungal genomes, was comparatively enriched. The comparative genome analysis showed that the genes related to sexual communication such as the biosynthesis of β-carotene and trisporic acid were conserved and diverged during the evolution of zygomycete genomes. Overall, these findings will help us to understand how zygomycetes are associated with plants. The kingdom Fungi has a critical role in the carbon cycle1 as well as various interactions with other living organ- isms. Zygomycetes diverged in the early stage of fungal evolution2,3 and have various ecological types, which encompass plant and animal pathogens4–9, saprobes10, and parasites of other fungal species11.
    [Show full text]
  • Studies on Choanephoraceae. 11. Effect of Hydrogen-Ion Concentration Madliav a D
    ZOBODAT - www.zobodat.at Zoologisch-Botanische Datenbank/Zoological-Botanical Database Digitale Literatur/Digital Literature Zeitschrift/Journal: Sydowia Jahr/Year: 1964 Band/Volume: 17 Autor(en)/Author(s): Mehrotra Madhava Das Artikel/Article: Studies on Choanephoraceae. II. Effect of Hydrogen-ion Concentation. 223-229 ©Verlag Ferdinand Berger & Söhne Ges.m.b.H., Horn, Austria, download unter www.biologiezentrum.at Studies on Choanephoraceae. 11. Effect of Hydrogen-ion Concentration Madliav a D. M e h r o t r a. (907, Kalyani Devi, Allahabad, India). With 2 fig. in the text. Hydrogen and hydroxyl-ions are present in all media and they exert a decided effect on the growth ajnd spoliation of fungi. Lilly and B ar n ett (1951) have pointed out that pH also influences many other life processes of a fungus. Foster (1949) slates that pll of the medium plays a part in determining whether a particular amino acid can be utilized. Besides, utilization of sugars has also been reported to be influenced by the pll of the culture medium. Raper et-al. (1953) reported that some fungi utilize glucose only when the pH of the medium is below (i, best pll for the same being 5.5. Further, they reported that the utilization of fructose takes place only in acidic medium and no growth occurs on a neutral fructose medium within two weeks. Fungi differ considerably in their tole- rance to different pH values. The growth of fungi may be completely inhibited in media, which are either too acidic or too alkaline. The growth on the acidic side has been recorded up to as low as pH 3 and on the alkaline side up to pH 8.0—9.0 by several investigators.
    [Show full text]
  • Pest Risk Analysis (PRA) for Grain and Seed of Beans, Phaseolus Vulgaris L
    Pest Risk Analysis (PRA) for Grain and Seed of Beans, Phaseolus vulgaris L. within East African Countries (Kenya, Burundi, Rwanda, Tanzania and Uganda) A Qualitative, Pathway-Initiated Risk Analysis Adopted by the 39th Meeting of the Council of Ministers held on 28th November, 2019 1 Executive Summary According to Kilimo trust, 2012, the leading producers of beans in Africa are, Tanzania, Uganda and Kenya whose production volumes in 2010 were estimated at 950,000MT, 455,000MT and 390,598MT respectively. The volumes traded across borders in the East African region stand at 309,000MT annually according to the East African cross border trade bulletin of 2018. This poses potential pest risks to the bean value chain and requires analysis to provide scientific justification for Phytosanitary conditions in beans. This Pest Risk Analysis (PRA) documents outlines risks associated with the movement of grain and seeds of beans, Phaseolus vulgalis L. within East African member countries namely: Kenya, Burundi, Rwanda, Tanzania and Uganda. The Beans PRA was initiated by the need to review the national pest lists and develop strategies for reducing Phytosanitary trade barriers in the East African region as well as develop a harmonized regional pest list for beans, with a view to developing phytosanitary import conditions that will be applied within the East African. The objectives of the Regional Pest Risk Analysis were to: review national pest lists for beans; develop a harmonized regional pest list for beans; develop a draft regional PRA; develop National Quarantine Pest Lists; and develop Phytosanitary import conditions for beans to be applied within the East African region.
    [Show full text]