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An Antiproliferative Ribonuclease from Fruiting Bodies of the Wild Mushroom Russula Delica
J. Microbiol. Biotechnol. (2010), 20(4), 693–699 doi: 10.4014/jmb.0911.11022 First published online 30 January 2010 An Antiproliferative Ribonuclease from Fruiting Bodies of the Wild Mushroom Russula delica Zhao, Shuang1,2, Yong Chang Zhao3, Shu Hong Li3, Guo Qing Zhang1, He Xiang Wang1*, and Tzi Bun Ng4* 1State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China 2Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China 3Institute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Science, Kunming 650223, China 4School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China Received: November 20, 2009 / Revised: December 21, 2009 / Accepted: December 25, 2009 An antiproliferative ribonuclease with a new N-terminal The mushroom family Russulaceae is composed of two sequence was purified from fruiting bodies of the edible genera, Russula and Lactarius, the former being the wild mushroom Russula delica in this study. This novel majority. To date, only a ribonuclease [34] and a protein ribonuclease was unadsorbed on DEAE-cellulose, but with anti-HIV-1 reverse transcriptase activity [38] have absorbed on SP-Sepharose and Q-Sepharose. It had a been isolated from mushrooms of the genus Russula. Only molecular mass of 14 kDa, as judged by fast protein liquid four reports on Lactarius lectin [6, 10, 24, 26] and one chromatography on Superdex 75 and SDS-polyacrylamide report on a Lactarius enzyme [17] are available. Russula gel electrophoresis. Its optimal pH and optimal temperature delica is a wild mushroom on which few literatures have were pH 5 and 60oC, respectively. -
Lactarius Deliciosus
© Saffron milk cap (Lactarius deliciosus) The saffron milk cap mushroom (Lactarius deliciosus) has been eaten in Europe since Roman times and is still greatly appreciated around the Meditteranean, particularly Spain, Portugal, Germany and elsewhere, for its fruity aroma and a “crisp flesh with nutty, earthy, and woodsy flavours”. It made the accidental journey to Australia probably on the roots of imported trees by early European settlers and is found in pine forests throughout the southeastern part of the country where it is collected for the restaurant and gourmet trade. It is easily recognised by the saffron-coloured sap it bleeds when damaged, the concentric rings of salmon-coloured blotches on the surface of the cap, and its tendency to turn green with age or after being handled. Grade 1 saffron milk cap typically wholesales for NZ$30 and A$40/kg whereas in exclusive shops in Europe prices can be higher. dry summers and removing low branches. The profitability of the plantation can be further boosted if the mushrooms are harvested and transported to the market corectly. Woven baskets with the mushrooms upside down (left) are used by a wild mushroom collectors near Melbourne. The first New Zealand saffron milk cap mycorrhized trees were planted in August 2000 and mushrooms were produced in coastal North Otago after only 18 months. The first commercial crop was sold in 2003 by Hannes and Theres Krummenacher near Nelson (Neudorf Mushrooms). In 2009 this plantation averaged 6 kg per tree and the total mushrooms produced per tree since planting far exceeds the value of the timber produced by a 30 year old well pruned radiata pine. -
Non-Wood Forest Products in Europe
Non-Wood Forest Products in Europe Ecologyand management of mushrooms, tree products,understory plants andanimal products Outcomes of theCOST Action FP1203 on EuropeanNWFPs Edited by HARALD VACIK, MIKE HALE,HEINRICHSPIECKER, DAVIDE PETTENELLA &MARGARIDA TOMÉ Bibliographicalinformation of Deutsche Nationalbibliothek [the German National Library] Deutsche Nationalbibliothek [the German National Library] hasregisteredthispublication in theGermanNationalBibliography. Detailed bibliographicaldatamay be foundonlineathttp: //dnb.dnb.de ©2020Harald Vacik Please cite this referenceas: Vacik, H.;Hale, M.;Spiecker,H.; Pettenella, D.;Tomé, M. (Eds)2020: Non-Wood Forest Products in Europe.Ecology andmanagementofmushrooms, tree products,understoryplantsand animal products.Outcomesofthe COST Action FP1203 on EuropeanNWFPs, BoD, Norderstedt,416p. Coverdesign, layout,produced andpublished by:BoD –Books on Demand GmbH, In de Tarpen 42,22848 Norderstedt ISBN:978-3-7526-7529-0 Content 5 1. Introduction.......................................................11 1.1Non-wood forest products.....................................11 1.2Providingevidencefor NWFP collection andusage within Europe ......................................14 1.3Outline of thebook...........................................15 1.4References ...................................................17 2. Identificationand ecologyofNWFPspecies........................19 2.1Introduction.................................................19 2.2 Theidentification of NWFP in Europe. ........................ -
<I>Russula Atroaeruginea</I> and <I>R. Sichuanensis</I> Spp. Nov. from Southwest China
ISSN (print) 0093-4666 © 2013. Mycotaxon, Ltd. ISSN (online) 2154-8889 MYCOTAXON http://dx.doi.org/10.5248/124.173 Volume 124, pp. 173–188 April–June 2013 Russula atroaeruginea and R. sichuanensis spp. nov. from southwest China Guo-Jie Li1,2, Qi Zhao3, Dong Zhao1, Shuang-Fen Yue1,4, Sai-Fei Li1, Hua-An Wen1a* & Xing-Zhong Liu1b* 1State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing 100101, China 2University of Chinese Academy of Sciences, Beijing 100049, China 3Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, Yunnan, China 4College of Life Science, Capital Normal University, Xisihuanbeilu 105, Haidian District, Beijing 100048, China * Correspondence to: a [email protected] b [email protected] Abstract — Two new species of Russula are described from southwestern China based on morphology and ITS1-5.8S-ITS2 rDNA sequence analysis. Russula atroaeruginea (sect. Griseinae) is characterized by a glabrous dark-green and radially yellowish tinged pileus, slightly yellowish context, spores ornamented by low warts linked by fine lines, and numerous pileocystidia with crystalline contents blackening in sulfovanillin. Russula sichuanensis, a semi-sequestrate taxon closely related to sect. Laricinae, forms russuloid to secotioid basidiocarps with yellowish to orange sublamellate gleba and large basidiospores with warts linked as ridges. The rDNA ITS-based phylogenetic trees fully support these new species. Key words — taxonomy, Macowanites, Russulales, Russulaceae, Basidiomycota Introduction Russula Pers. is a globally distributed genus of macrofungi with colorful fruit bodies (Bills et al. 1986, Singer 1986, Miller & Buyck 2002, Kirk et al. -
Preliminary Investigations of the Content And
R-09 PRELIMINARY INVESTIGATIONS OF THE CONTENT AND LOCALIZATION OF CHITIN IN SELECTED SPECIES OF PILEUS FUNGI Janina Fiema, Janusz Kalbarczyk1, Bernarda Piskorz–Bińczycka2 The F. Górski Institute of Plant Physiology, Polish Academy of Sciences, ul. Niezapominajek 21, 30-239 Kraków, Poland 1 Fruits and Vegetables Processing Department, Agriculture Academy, ul. Skromna 8, 20-704 Lublin, Poland 2 Faculty of Biology and Agriculture, University of Rzeszów, ul. Ćwiklińskiej, Rzeszów, Poland 1. Introduction From literature data concerning the level of the content of radioactive cesium (137Cs) and potassium (40K) in the fungi it follows that Xerocomus badius was one of the more polluted species of fungi in Poland (data from the year 1998) [1]. It will be interesting to explain the existing differentiation as well as the protective mechanism among the various fungi against the environmental pollution. From the authors’ own investigations [4, 5] as well as from other literature data it follows that looking for an answer should be concentrated on the investigation of the structure of the cell walls, which form a barrier between the inside of the cell and the outer environment especially on such parts of the component walls – certain polysaccharides such as chitin and chitosan, From the recent reports it is already known that: 1) chitosan may be the sorbent of noble metals [2]. 2) absorption of toxic substances by the mycorrhize fungi is related with the composition of their cell walls [3]. 3) increased content of chitin in the cell walls of Aspergillus giganteus mut. alba in cultures grown in light [4, 5] protects the fungi against the detrimental influence of metals [6]. -
Phd. Thesis Sana Jabeen.Pdf
ECTOMYCORRHIZAL FUNGAL COMMUNITIES ASSOCIATED WITH HIMALAYAN CEDAR FROM PAKISTAN A dissertation submitted to the University of the Punjab in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY in BOTANY by SANA JABEEN DEPARTMENT OF BOTANY UNIVERSITY OF THE PUNJAB LAHORE, PAKISTAN JUNE 2016 TABLE OF CONTENTS CONTENTS PAGE NO. Summary i Dedication iii Acknowledgements iv CHAPTER 1 Introduction 1 CHAPTER 2 Literature review 5 Aims and objectives 11 CHAPTER 3 Materials and methods 12 3.1. Sampling site description 12 3.2. Sampling strategy 14 3.3. Sampling of sporocarps 14 3.4. Sampling and preservation of fruit bodies 14 3.5. Morphological studies of fruit bodies 14 3.6. Sampling of morphotypes 15 3.7. Soil sampling and analysis 15 3.8. Cleaning, morphotyping and storage of ectomycorrhizae 15 3.9. Morphological studies of ectomycorrhizae 16 3.10. Molecular studies 16 3.10.1. DNA extraction 16 3.10.2. Polymerase chain reaction (PCR) 17 3.10.3. Sequence assembly and data mining 18 3.10.4. Multiple alignments and phylogenetic analysis 18 3.11. Climatic data collection 19 3.12. Statistical analysis 19 CHAPTER 4 Results 22 4.1. Characterization of above ground ectomycorrhizal fungi 22 4.2. Identification of ectomycorrhizal host 184 4.3. Characterization of non ectomycorrhizal fruit bodies 186 4.4. Characterization of saprobic fungi found from fruit bodies 188 4.5. Characterization of below ground ectomycorrhizal fungi 189 4.6. Characterization of below ground non ectomycorrhizal fungi 193 4.7. Identification of host taxa from ectomycorrhizal morphotypes 195 4.8. -
A Review of the Occurrence of Alpha-Emitting Radionuclides in Wild Mushrooms
International Journal of Environmental Research and Public Health Review A Review of the Occurrence of Alpha-Emitting Radionuclides in Wild Mushrooms 1, 2,3, Dagmara Strumi ´nska-Parulska * and Jerzy Falandysz y 1 Toxicology and Radiation Protection Laboratory, Faculty of Chemistry, University of Gda´nsk, 80-308 Gda´nsk,Poland 2 Environmental Chemistry & Ecotoxicology Laboratory, Faculty of Chemistry, University of Gda´nsk, 80-308 Gda´nsk,Poland; [email protected] 3 Environmental and Computational Chemistry Group, School of Pharmaceutical Sciences, Zaragocilla Campus, University of Cartagena, Cartagena 130015, Colombia * Correspondence: [email protected]; Tel.: +48-58-5235254 Jerzy Falandysz is visiting professor at affiliation 3. y Received: 22 September 2020; Accepted: 3 November 2020; Published: 6 November 2020 Abstract: Alpha-emitting radioisotopes are the most toxic among all radionuclides. In particular, medium to long-lived isotopes of the heavier metals are of the greatest concern to human health and radiological safety. This review focuses on the most common alpha-emitting radionuclides of natural and anthropogenic origin in wild mushrooms from around the world. Mushrooms bio-accumulate a range of mineral ionic constituents and radioactive elements to different extents, and are therefore considered as suitable bio-indicators of environmental pollution. The available literature indicates that the natural radionuclide 210Po is accumulated at the highest levels (up to 22 kBq/kg dry weight (dw) in wild mushrooms from Finland), while among synthetic nuclides, the highest levels of up to 53.8 Bq/kg dw of 239+240Pu were reported in Ukrainian mushrooms. The capacity to retain the activity of individual nuclides varies between mushrooms, which is of particular interest for edible species that are consumed either locally or, in some cases, also traded on an international scale. -
Prospecting Russula Senecis: a Delicacy Among the Tribes of West Bengal
Prospecting Russula senecis: a delicacy among the tribes of West Bengal Somanjana Khatua, Arun Kumar Dutta and Krishnendu Acharya Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata, West Bengal, India ABSTRACT Russula senecis, a worldwide distributed mushroom, is exclusively popular among the tribal communities of West Bengal for food purposes. The present study focuses on its reliable taxonomic identification through macro- and micro-morphological features, DNA barcoding, confirmation of its systematic placement by phylogenetic analyses, myco-chemicals and functional activities. For the first time, the complete Internal Transcribed Spacer region of R. senecis has been sequenced and its taxo- nomic position within subsection Foetentinae under series Ingratae of the subgen. Ingratula is confirmed through phylogenetic analysis. For exploration of its medic- inal properties, dried basidiocarps were subjected for preparation of a heat stable phenol rich extract (RusePre) using water and ethanol as solvent system. The an- tioxidant activity was evaluated through hydroxyl radical scavenging (EC50 5 µg/ml), chelating ability of ferrous ion (EC50 0.158 mg/ml), DPPH radical scavenging (EC50 1.34 mg/ml), reducing power (EC50 2.495 mg/ml) and total antioxidant activity methods (13.44 µg ascorbic acid equivalent/mg of extract). RusePre exhibited an- timicrobial potentiality against Listeria monocytogenes, Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus. Furthermore, diVerent parameters were tested to investigate its chemical composition, which revealed the presence of appreciable quantity of phenolic compounds, along with carotenoids and ascorbic acid. HPLC- UV fingerprint indicated the probable existence of at least 13 phenolics, of which 10 were identified (pyrogallol > kaempferol > quercetin > chlorogenic acid > ferulic Submitted 29 November 2014 acid, cinnamic acid > vanillic acid > salicylic acid > p-coumaric acid > gallic acid). -
Researches on Russulaceous Mushrooms-An Appraisal Reported to Provide Some Non-Nutritional Benefits to Tree 1932, 1940) and Beardslee (1918)
63 KAVAKA47 : 63 - 82 (2016) Researches on Russulaceous Mushrooms-AnAppraisal N.S.Atri,Samidha Sharma* , Munruchi Kaur Sainiand Kanad Das ** Department of Botany, PunjabiUniversity, Patiala 147002, Punjab, India. *Department of Botany, Arya College, Ludhiana 141001, Punjab, India. **Botanical Survey of India, Cryptogamic Unit, P.O. BotanicGarden, Howrah 711103, India Corresponding author email: [email protected] (Submitted onAugust 10, 2016 ;Accepted on October 2, 2016) ABSTRACT Russulaceae is one among the large families of the basidiomycetous fungi. Some significant studies during the last decade on their systematics and molecularphylogenyresulted in splittingof well knownmilkcapgenusLactarius s.l.andinclusionof number of gastroid and resupinate members under its circumscription. Presently, there are seven genera (including agaricoid, gasteroid and resupinate members) in this family viz. RussulaPers. , Lactarius Pers. , Lactifluus (Pers.) Roussel, Cystangium Singer & A.H. Smith , Multifurca Buyck & Hofst., Boidinia Stalper & HjortstamandPseudoxenasma K.H.Larss.&Hjortstamspreadover 1248+ recognisedspeciestheworldover.Outof atotalofabout 259 + species/taxaofRussulacousmushrooms,146taxaofRussula ,83taxaof Lactarius ,27taxaof Lactifluus ,2speciesof Boidinia and1speciesof Multifurca are documented from India. In this manuscript an appraisal of the work done on various aspects of the members of the family Russulaceae including their taxonomic, molecular,phylogenetic, scanning electron microscopic, ectomycorrhizal, nutritional and nutraceutical aspects has been attempted. Keywords: Russulaceae, taxonomy, phylogeny, SEM, ECM, nutritional, nutraceutical, review INTRODUCTION taxa ofRussula and 83 taxa of Lactarius, 27 taxa of Lactifluus (Pers.) Roussel are known. (Atri et al ., 1994; Das and Sharma, The familyRussulaceae Lotsy is one of the 12 families under 2005; Bhattet al ., 2007; Das 2009; Das et al ., 2010, 2013, orderRussulales Krisel ex P.M. Kirk, P.F. Cannon & J.C. 2015; Das and Verbeken, 2011, 2012; Lathaet al ., 2016; David (Kirket al ., 2008). -
Mushrooms Russia and History (Pdf)
Mushrooms Russia and History by Valentina Pavlovna Wasson and R. Gordon Wasson Volume I and II Manufactured in Italy for the authors and Pantheon Books Inc. 333, Sixth Avenue, New York 14, N. Y. © 1957 by R. Gordon Wasson original text: http://www.newalexandria.org/archive/MUSHROOMS%20RUSSIA%20AND%20HISTORY%20Volume%201.pdf backup source: http://www.psilosophy.info/resources/MUSHROOMS%20RUSSIA%20AND%20HISTORY%20Volume%201.pdf original text: http://www.newalexandria.org/archive/MUSHROOMS%20RUSSIA%20AND%20HISTORY%20Volume%202.pdf backup source: http://www.psilosophy.info/resources/MUSHROOMS%20RUSSIA%20AND%20HISTORY%20Volume%202.pdf Changes to this edition: 1. Cyrillic has been added to the first occurrence of a simplified Russian pronunciation of a word. For example togrib , cyrillic is added in parenthesis - (гриб). 2. In chapter I. Mushrooms and the Russians, where authors mention about folk names for mushrooms, actual Latin name has been found and inserted into square brackets (but beside Appendix II where authors do this by themselves) for most of this names. Thus the name originally presented as volnushki will be volnushki (волнушки) [Lactarius torminosus]. 3. Footnotes are numbered continuously, contrary to original version where footnote number starts from 1 on each page. 4. Latin names have been italicized. 5. Some latin synonyms are actuallized beneath plates, eg. Psalliota campestris Fr. ex L. has in description additionaly [Agaricus campestris (Bull.)]. 6. Polish official names for mushrooms have been added beneath plates. 7. Couple of notes have been added and labeled as Note to this edition of the book on Psilosophy. 8. Illustrations have been whitened. -
Alba-TFM.Pdf (5.908Mb)
“ENTRA A DESCUBRIR EL FASCINANTE MUNDO DE LOS HONGOS” ANÁLISIS DEL TURISMO MICOLÓGICO EN ANDALUCÍA Página 1 ÍNDICE 1. INTRODUCCIÓN 4 2. OBJETIVO GENERAL 5 3. OBJETIVOS ESPECÍFICOS 5 4. CONCEPTOS BÁSICOS, DEFINICIÓN DE MICOLOGÍA Y TURISMO MICOLÓGICO 5 5. HISTORIA DE LA MICOLOGÍA 8 6. EVOLUCIÓN DE LA ACTIVIDAD MICOLÓGICA EN ESPAÑA 12 6.1. ESTUDIO DE LA OFERTA DEL TURISMO MICOLÓGICO 13 6.2. ESTUDIO DE LA DEMANDA DEL TURISMO MICOLÓGICO 18 7. DIAGNÓSTICO DEL POTENCIAL DEL MICOTURISMO PARA EL DESARROLLO DEL MEDIO RURAL 20 8. INICIATIVAS DESARROLLADAS PARA EL APROVECHAMIENTO MICOLÓGICO EN ESPAÑA. PRINCIPALES PROYECTOS MICOLÓGICOS EN EL ÁMBITO 23 8.1. INICIATIVASS PUESTAS EN MARCHA PARA LA GESTIÓN ACTIVA DEL RECURSO MICOLÓGICO ANDALUZ 31 9. ESTUDIO DE ANDALUCÍA COMO DESTINO MICOLÓGICO 48 9.1 ANÁLISIS FÍSICO DE ANDALUCÍA, ESCENARIO DE NUESTRO TRABAJO 48 9.2. ANÁLISIS DE LA OFERTA MICOTURÍSTICA EXISTENTE EN ANDALUCÍA 53 9.3. ASOCIACIONES MICOLÓGICAS, SOCIEDADES MICOLÓGICAS, Y AGRUPACIONES MICOLÓGICAS EXISTENTES EN ANDALUCIA 76 10. ANÁLISIS DE UN DESTINO MICOLÓGICO CONSOLIDADO – CASTILLA Y LEÓN 96 11. REPERCUSIÓN SOCIAL Y ECONÓMICA DEL APROVECHAMIENTO MICOLÓGICO 132 12. PROPUESTA DE INICIATIVAS DE DESARROLLO LOCAL A TRAVÉS DE LA PUESTA EN VALOR DEL RECURSO ENDÓGENO 133 12. CONCLUSIONES 136 ANÁLISIS DEL TURISMO MICOLÓGICO EN ANDALUCÍA Página 2 ANEXOS 138 BIBLIOGRAFÍA 172 BIBLIOWEB 173 ANÁLISIS DEL TURISMO MICOLÓGICO EN ANDALUCÍA Página 3 1. INTRODUCCIÓN En los últimos años, la sociedad Española ha experimentado una mayor sensibilidad hacia los temas medioambientales, debiéndose esto a la confluencia de una serie de factores que están cambiando el rumbo del medio rural. -
Effect of Different Drying Method on Volatile Flavor Compounds Of
rocess P ing od & o T F e f c Huang et al., J Food Process Technol 2016, 7:8 o h l n a o n l r o Journal of Food DOI: 10.4172/2157-7110.1000615 u g o y J ISSN: 2157-7110 Processing & Technology Research Article Open Access Effect of Different Drying Method on Volatile Flavor Compounds of Lactarius deliciosus Qun Huang, Lei Chen, Hong-bo Song*, Feng-ping An, Hui Teng and Mei-yu Xu College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, China Abstract The effect of different drying methods on volatile flavor compounds of Lactarius deliciosus was investigated, such as hot- air drying, vacuum freeze drying and sunshine drying. By adopting the solid-phase micro-extraction method, volatile flavor compounds were extracted from lactarius deliciosus, then analyzed and identified through the application of gas chromatography- mass spectrometer (GC-MS). Results indicated that different drying methods could lead to large differences in volatile flavor compounds. Main volatile flavor compounds of fresh Lactarius deliciosus involved acids and aldehydes, which accounted for 86.31%; Hot-air dried Lactarius deliciosus mainly included acids and alkene, which accounted for 87.16%; Lactarius deliciosus dried with vacuum freezed Lactarius deliciosus was mostly composed of acids, esters and aromatic substance, which accounted for 94.74%; Lactarius deliciosus dried with sunshine was constituted by acid and aldehydes, which was as high as 90.67%. Keywords: Lactarius deliciosus; Volatile flavor compounds; Drying deliciosus by extracting with head space solid phase micro extraction method; Gas chromatography-mass spectrometer (GC-MS) (SPME) technique and analyzing with gas chromatography-mass spectrometry (GC-MS).