INTERNATIONAL JOURNAL OF PHARMACEUTICAL AND CHEMICAL SCIENCES ISSN: 22775005

Review Article Phytochemical and Pharmacological Review on Nobilis

Ramling Patrakar*, Meera Mansuriya and Priyanka Patil

Shree Santkrupa College of Pharmacy, Ghogaon (karad),Maharashtra, India. ______ABSTRACT is traded as sweet bay leaf, and true, Roman, or Turkish laurel. It is small evergreen of family. Chemically it has found to contain sesquiterpene lactones such as 10- epigazaniolide, Gazaniolide, spirafolide, costunolide, Reynosin, santamarine, flavonoid glycosides, essential oil. It has been reported to possess wound healing, neuroprotective, antioxidant, antiulcerogenic, anticonvulsant, antimutagenic, antiviral, anticholinergic, antibacterial, antifungal activities. This review highlighted some Phytochemical and Pharmacological aspect of Laurus nobilis which will be useful to the researcher for further study.

Keywords: Cultivation and collection, Laurus nobilis, Phytochemistry, Pharmacology, .

INTRODUCTION diuretic9. Recently it is used in treating Laurus nobilis is traded as sweet bay leaf, diabetes and preventing migraine10. and true, Roman, or Turkish laurel. It is small evergreen tree of lauraceae family. Taxonomical classification It is hardy multibranched tree with smooth Kingdom: Plantae bark that grows to about 10 m high1,2. It Division: Magnolids has alternate, narrowly oblong-lanceolate Order: leaves. The are small and four Family: Lauraceae lobed; the male has 8—12 stamens and Genus: Laurus female 2—4 staminodes. The is 10— Species: Laurus nobilis 15 mm, ovoid, and black when ripe3. These are aromatic and fragrant Cultivation and collection yielding fixed and volatile oil as well as Sweet bay is propagated by seeds or camphor, it is native of south Europe4. preferably by cuttings. From a well ripened Laurus nobilis is a of industrial wood, cuttings of about 7.5 to 10 cm importance, used in foods, drugs, and length are put in sharp sand either under cosmetics. The dried leaves and essential bell-glasses or in glass cases. The rooted oils are used extensively in the food cuttings are placed in small pots industry for seasoning of meat products, containing fairly rich sandy loam with good soups and fishes. Its antimicrobial and drainage, and then can be put in a hot insecticidal activities are other factor for bed, with gentle bottom heat where they which bay is used in the food industry as a will make a good strong growth. L. nobilis food preservative. The contain both stem cuttings produce roots better in July/ fixed and volatile oils, which are mainly August, under Mediterranean conditions, used in soap making5. Traditionally it is than in other seasons, although the used in rheumatism and dermatitis6, optimal rooting period can be extended by gastrointestinal problems, such as bottom heating from May until September. epigastric bloating, impaired digestion, After that, they may be planted in nursery eructation, and flatulence, The aqueous beds with rich sandy soil and good extract is used in Turkish folk medicine as drainage. In one growing season, the an anti-hemorrhoidal, anti-rheumatic, plants may attain a height of 1 to 1.5 m. At diuretic, as an antidote in snakebites and the end of the growing season and long for the treatment of stomachache7,8 before the cold season the young plants together with their stakes are kept in well

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INTERNATIONAL JOURNAL OF PHARMACEUTICAL AND CHEMICAL SCIENCES ISSN: 22775005 lit and ventilated sheds and temperature is Glycosides kept just above Laurus nobilis leaves yielded four non- freezing. These plants are kept in close polar flavonoids kaempferol-3-O-α-L- rows and watered once or twice a week. (3",4"-di-E-p-coumaroyl)- rhamnoside, The plants are taken out during the spring kaempferol-3-O-α-L-(2",4"-di-E-p- season and either potted or plunged in coumaroyl)-rhamnoside, kaempferol-3-O- nursery. The rich peaty soil with plenty of α-L-(2"-4"-pcoumaroyl)- rhamnoside and a water and congenial moist atmosphere new product kaempferol-3-O-α-L-(2",4"-di- near the sea coast are favorable Z-p-coumaroyl)-rhamnoside [20] .Five new conditions for fast and luxriant growth1. megastigmane glucosides named The leaves of L. nobilis are plucked and laurosides A−E and a new phenolic dried under shade for use as a flavouring glucoside were isolated from the material in a variety of culinary methanolic extract of L. nobilis L. leaves21. preparations, especially in French cuisine. Kaempferol- 3-rhamnopyranoside, and The leaves contain an essential oil of kaempferol-3, 7- di-rhamnopyranoside aromatic, spicy odour and flavour which were isolated from Laurus nobilis aqueous can be isolated by ethanolic extract22. steam distillation. The oil is a valuable adjunct in the flavouring of all kinds of Anthocyanin food products, particularly meats, The major anthocyanins were sausages, canned soups, baked goods, characterized as cyanidin 3-O-glucoside confectionery, etc. The oil replaces the and cyanidin 3-O-rutinoside. Furthermore, dried leaves to great advantage because it two minor anthocyanins were detected can be dosed more exactly and therefore and identified as 3-O-glucoside and 3-O- gives more uniform results than the dried rutinoside23. leaves12. Essential oil PHYTOCHEMISTRY The main components of the oil were Terpenoids identified. 1,8-Cineole along with α-terpinyl Various sesquiterpene lactones were acetate, terpinene-4- ol, α-pinene, β- found to present in Laurus nobilis such as pinene, p-cymene, linalool acetate24 .It 10-epigazaniolide, Gazaniolide, also found to contain (E)-β-cymene, β- spirafolide, costunolide, Reynosin and longipinene, cadinene, α-terpinyl acetate, santamarine[13], 5a,9-dimethyl-3- α-bulnesene25, terpinene-4-ol (4.25%), methylene-3,3a,4,5,5a,6,7,8-octahydro-1- sabinene. The acyclic monoterpenes oxacyclopenta[c]azulen-2-one and 3β – linalool and myrcenol were present in chlorodehydrocostuslactone along with smaller amounts, while cumin aldehyde, other sesquiterpene lactones such as dimethylstyrene, eugenol, methyl eugenol, dehydrocostuslactone, artremorinand[14], and carvacrol were found 26. Lauroxepine, 11,13-dehydrosantonin[15], 5a,9-dimethyl-3-methylene- PHARMACOLOGY 3,3a,4,5,5a,6,7,8-octahydro-1- Wound healing activity oxacyclopenta[c]azulen-2-one and 3β- The aqueous extracts of Laurus nobilis chlorodehydrocostuslactone[14], deacetyl were evaluated for its wound healing laurenobiolide[16], 5αH,7αH-eudesman- activity in rats and compared with 4α,6α,11,12-tetraol and 1β,15-dihydroxy- aqueous extract of Allamanda. Excision 5αH,7αH-eudesma-3,11(13)-dien-12,6α- and incision wound models were used to olide[17], Trypanocidal terpenoid evaluate the wound healing activity. zaluzanin D[18]. Two steroisomeric wound healing were assessed by the rate monoterpine alcohol such as Cis and of wound closure, period of trans-thuj-2-en4-ol were obtained in the epithelialisation, tensile strength, weights essential oil of Laurus nobilis[19]. of the granulation tissue, hydroxyproline content and histopathology of the

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INTERNATIONAL JOURNAL OF PHARMACEUTICAL AND CHEMICAL SCIENCES ISSN: 22775005 granulation tissue. In Laurus nobilis extracts shown strong total antioxidant treated animals, the rate of wound activity in linoleic acid emulsion. contraction, weight of the granulation Concentrations of 20, 40, and 60 µg/ ml tissue and hydroxyproline content were showed 84.9, 95.7, 96.8, and 94.2, 97.7, moderately high (P < .05). The histological and 98.6% inhibition of lipid peroxidation study of the granulation tissue of the of linoleic acid emulsion, for water and Laurus nobilis treated animals showed ethanol extracts, respectively. On the larger number of inflammatory cells, and other hand, 60 µg/ ml of the standard lesser collagen when compared with the antioxidants butylated hydroxyianisole Allamanda cathartica treated group of (BHA), butylated hydroxytoluene (BHT), animals27 . and α-tocopherol exhibited 96.6, 99.1, and 76.9% inhibition of lipid peroxidation in Neuroprotective activity linoleic acid emulsion, respectively. In The effects of n-hexane fraction from addition, the both extracts were having Laurus nobilis leaves on dopamine effective reducing power, DPPH· free induced intracellular reactive oxygen radical scavenging, superoxide anion species (ROS) production and apoptosis radical scavenging, hydrogen peroxide in human neuroblastoma SH-SY5Y cells scavenging and metal chelating activities was investigated. Compared with at 20, 40, and 60 µg/ ml. The total amount apomorphine (APO, IC50=18.1 µM) as a of phenolic compounds in each extract positive control, IC50 value of hexane was determined as gallic acid fraction for DA-induced apoptosis was 3.0 equivalents29. µg/ml, and two major compounds from, costunolide and dehydrocostus lactone, Antiulcerogenic activity were 7.3 µM and 3.6 µM, respectively. Antiulcerogenic activity of Laurus nobilis Hexane fraction and these major seeds was tested on experimentally compounds significantly inhibited ROS ethanol induced gastric ulcer in rats. The generation in DA-induced SH-SY5Y cells. results indicated antiulcerogenic activity A rodent 6-hydroxydopamine (6-OHDA) for 20 and 40% aqueous extracts as well model of PD was employed to investigate as for the oily fraction of these seeds30. the potential neuroprotective effects of hexane fraction in vivo. 6-OHDA was Anticonvulsant activity injected into the substantia nigra of young The leaf essential oil of Laurus nobilis was adult rats and an immune histochemical evaluated for anticonvulsant activity analysis was conducted to quantitate the against experimental seizures. The tyrosine hydroxylase (TH)-positive essential oil protected mice against tonic neurons. Hexane fraction signifi cantly convulsions induced by maximal inhibited 6-OHDA-induced TH-positive cell electroshock and especially by loss in the substantia nigra and also pentylenetetrazole. Components reduced DA induced α-synuclein (SYN) responsible for this effect may be formation in SH-SY5Y cells and shown to methyleugenol, eugenol and pinene be neuroprotective28. present in the essential oil. At anticonvulsant doses, the essential oil Antioxidant Activity produced sedation and motor impairment. Lyophilized aqueous and ethanol extract This effect seems because of cineol, of Laurus nobilis were studied for their eugenol and methyleugenol31. antioxidant properties. The antioxidant activity, reducing power, free radical Analgesic and anti-inflammatory scavenging, superoxide anion radical The leaf essential oil of Laurus nobilis scavenging, hydrogen peroxide Linn. evaluated for analgesic and anti- scavenging and metal chelating activities inflammatory activities in mice and rats. were evaluated to determine the total The essential oil exhibited a significant antioxidant capacity of both extracts. Both analgesic effect in tail-flick and formalin

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INTERNATIONAL JOURNAL OF PHARMACEUTICAL AND CHEMICAL SCIENCES ISSN: 22775005 tests, a dose-dependent anti-inflammatory activity as this study showed no effect on effect in the formalin-induced edema and respiratory burst, lysozyme activity and a moderate sedative effect at the anti- total plasma protein levels35. inflammatory doses. The analgesic and anti-inflammatory effect of the essential oil Antiviral activity was comparable to reference analgesics Essential oils of Laurus nobilis were and non-steroid anti-inflammatory drugs evaluated for their inhibitory activity such as morphine and piroxicam32. against SARS-CoV and HSV-1 replication Ethanol, aqueous extracts obtained from in vitro by visually scoring of the virus- leaves and seeds of Laurus nobilis were induced cytopathogenic effect post- also studied using carrageenan-induced infection. Laurus nobilis oil shown activity hind paw edema model in mice without against SARS-CoV with an IC (50) value inducing any gastric damage for anti- of 120 µg /ml and a selectivity index (SI) of inflammatory activity and ethanol extract 4.16. This oil was characterized by the shown prominent anti-inflammatory presence of beta-ocimene, 1, 8-cineole, activity33. alpha-pinene, and beta-pinene as the main constituents36. Antimutagenic activity The antimutagen was purified Anticholinergic activity chromatographically from ethyl acetate Essential oil, ethanolic extract and extract of bay leaf and identified decoction of Laurus nobilis were analyzed instrumentally to be 3-kaempferyl p- for their activity towards acetyl coumarate. The yield was 20 mg from 100 cholinesterase (AChE) enzyme. It showed g of bay, and its IC50 value, the amount AChE inhibitory capacity higher than 50% required for 50% inhibition of the in the essential oil fraction. It also showed mutagenicity of 20 mg of Trp-P-2, was 1.9 a high inhibition value of AChE in the µg. This value is close to those for strong ethanolic fraction, 64% (1 mg ml−1)37. antimutagens such as flavones and flavonols. The antimutagenicity was due to Insect repellent activity a desmutagenic action that inhibited the Essential oils extracted from the seeds of metabolic activation of Trp-P-2 to its fresh foliage of laurel Laurus nobilis Linn. ultimate carcinogenic form. The were tested for their repellent activity kaempferyl moiety contributed to the 34 against the adult females of Culex pipiens, activity . usually the most common pest mosquito in urban and suburban settings in the Immunostimulant activity Antalya province. The essential oils Immunostimulant effects of laurel powder showed repellent activity38. investigated by dietary intake on rainbow trout. After 14 days of adaptation on a Antibacterial activity control diet 3 groups of rainbow trout were In vitro antibacterial activities of the fed the experimental diets containing 0.5 essential oil, seed oil, and methanolic and 1% laurel leaf powder for 21 days. extract of seed oil obtained from Laurus The fish were then switched back to the nobilis was carried. The methanolic control diet. Non-specific immunity was extract of seed oil exhibited more effective investigated at the end of the 21 day antibacterial activity comparing to experimental feeding period and then essential oil and seed oil39. again 42nd, 63rd days later. The non- specific immune parameters, extracellular and intracellular respiratory burst Antifungal activity activities, phagocytosis in blood Antifungal activity of Laurus nobilis were leukocytes, lysozyme and total plasma carried out in vitro on seven strains of protein level were evaluated. Laurus plant pathogenic fungi cultivated in Petri nobilis is not having immunostimulant plates and each extract (or essential oil)

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INTERNATIONAL JOURNAL OF PHARMACEUTICAL AND CHEMICAL SCIENCES ISSN: 22775005 was tested at three different was significantly reduced to 51%, while concentrations (50, 125 and 250 µg/ml). dilutions of 2.5%, 1.25% and 0.625% were The results has been demonstrated that ineffective41. the greatest antifungal activity was observed for the extract obtained from L. CONCLUSION nobilis against the Botrytis cinerea This study attempts to focus the Pers. when it was applied at an Therapeutic potential of Laurus nobilis and concentration of 250 µg/ml40. their constituents. From this study we can conclude that the results reviewed in the Acaricidal activity review artical will be helpful of researchers Acaricidal activity of Laurus nobilis leaf seeking new drugs from Laurus nobilis. oils against Psoroptes cuniculi evaluated. The isolated compounds will be Acaricidal activity of L. nobilis oil, at a considered in future for more clinical concentration of 10%, led to a mortality evaluations. rate of 73%; at 5% the average activity

Fig. 1: Laurus nobilis plant

Gazaniolide Costunolide Santamarine

Reynosin 11, 13-dehydrosantonin Spirafolide

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Lauroxepine 3β-chlorodehydrocostuslactone Zaluzanin D(R=OAc) Fig. 2: Structures of some phytochemicals isolated from Laurus nobilis REFERENCES 12. Guenther E. The Essential Oils; In: Oil 1. Bailey LH. The Standard Cyclopedia of of Bay. Van Nostrand Company Inc, Horticulture. The Macmillan Company, New York; 1953:378–396. New York; 1963:182–187. 13. Fang F, Sang S, Kuang YC, Alexander 2. Ivan AR. Medicinal plants of the world; G, Chi-Tang H, Robert TR. Isolation in: chemical constituent, traditional and and identification of cytotoxic modern medicinal uses. Humana compounds from Bay leaf (Laurus press Totowa, new jersy; 2001: 261. nobilis). Food Chemistry. 2005; 3. Pignatti S. Flora d’Italia. Edagricole, 93:497–501. Bologna, Italy; 1982:233-234. 14. Dallacqua S, Viola G, Giorgetti M, Loi 4. John HB. The plants of the Bible, MC, Innocenti G. Two New and shrubs. Nelson and sons, London; Sesquiterpene Lactones from the 1857:5-6. Leaves of Laurus nobilis. Chem. 5. Bozan B, Karakaplan U. Antioxidants Pharm. Bull. 2006; 54(8): 1187-1189. from laurel (Laurus nobilis L.) berries: 15. Ash B, Topcu G, Oksuz S, Tume G, influences of extraction procedure on Kingston D. Identification of cytotoxic yield and antioxidant activity of sesquiterpenes from Laurus nobilis L. extracts. Acta Alim. 2007; 36: 321-328. Food Chemistry. 2007; 104:1478– 6. Kilic A, Hafizoglu H, 1484. Kollmannsberger H, Nitz S. Volatile 16. Fukuyama N, Ino C, Suzuki Y, constituents and key odorants in Kobayashi N, Hamamoto H, Sekimizu leaves, buds, flowers, and fruits of K, Orihara Y. Antimicrobial Laurus nobilis L. J. Agric. Food Chem. sesquiterpenoids from Laurus nobilis 2004; 52: 1601-1606. L. Nat Prod Res. 2011; 1: 1-9. 7. Gulcin I. Toxicology: 213,(2006) 17. Marino SD, Borbone N , Zollo F, 8. Baytop T. Therapy with Medicinal Ianaro A , Meglio PD, Iorizzi M . New Plants in Turkey. Publications of Sesquiterpene Lactones from Laurus Istanbul University, Istanbul; 1984:194. nobilis Leaves as Inhibitors of Nitric 9. Aqili MS. Collection of drugs (Materia Oxide Production. Planta Med. 2005; media). Enqelab-e-Eslami Publishing 71(8): 706-710. and Educational Organization, Tehran; 18. Uchiyama N, Matsunaga K, Kiuchi 1992: 624–630, F, Honda G, Tsubouchi A, Nakajima- 10. Duke JA. The green pharmacy; In: Shimada J, Aoki T. Trypanocidal New discoveries in herbal remedies for Terpenoids from Laurus nobilis L. common diseases and conditions from Chem. Pharm. Bull,50(11): 1514— the world’s foremost authority on 1516 ,(2002) healing herbs. Rodale Press, New 19. Novak M. A monoterpene alcohol from York; 1997:240-241. laurus nobills. Phytochemistry. 1985; 11. Raviv M, Putievsky F, Ravid W, 24(4):858. Sanderovich D. Bay laurel as an 20. Christel F, Bruno D, Isabelle F, Joseph ornamental plant. Acta Hortic. 1983; V. Acylated kaempferol glycosides 132: 35–42. from laurus nobilis Leaves. Phytochernisty. 1998; 47(5): 821-824.

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21. Marino SD, Borbone N , Zollo F, 30. Afifi FU, Khalil E, Tamimi SO, Disi A. Ianaro A , Meglio PD, Iorizzi M . Evaluation of the gastroprotective Megastigmane and Phenolic effect of Laurus nobilis seeds on Components from Laurus nobilis L. ethanol induced gastric ulcer in rats. Leaves and Their Inhibitory Effects on Journal of Ethnopharmacology. 1997; Nitric Oxide Production. J. Agric. Food 58: 9-14. Chem. 2004; 52 (25): 7525–7531. 31. Sayyah M, Valizadeh J, Kamalinejad 22. Emam AM, Mohamed MA, Diab YM, M. Anticonvulsant activity of the leaf Megally NY. Isolation and structure essential oil of Laurus nobilis against elucidation of antioxidant compounds pentylenetetrazole- and maximal from leaves of Laurus nobilis and electroshock-induced seizures. Emex spinosus. Drug Discoveries and Phytomedicine. 2002; 9: 212–216. Therapeutics. 2010; 4(3):202-207. 32. Sayyah M, Saroukhani G, Peirovi A, 23. Luigia L, Giuseppe V. Anthocyanins Kamalinejad M. Analgesic and anti- from Bay (Laurus nobilis L.) Berries, J. inflammatory activity of the leaf Agric. Food Chem. 2005; 53(20): essential oil of Laurus nobilis Linn. 8063–8067. Phytotherapy Research. 2003; 24. Rizi MV. Chemical Composition and 17(7):733–736. Larvicidal Activity of the Essential Oil 33. Esra K, Ilkay O, Erdem Y. Evaluation of Laurus nobilis L. from Iran. Iranian of Some Plants Used in Turkish Folk Journal of Pharmaceutical Sciences. Medicine for Their Anti-inflammatory 2009; 5(1): 47-50. and Antinociceptive Activities. 25. Marzouki H, Piras A, Marongiu B, Pharmaceutical biology.2007; 45(7): Rosa A, Assunta M. Extraction and 547-555. Separation of Volatile and Fixed Oils 34. Samejima K, Kanazawa K, Ashida H, from Berries of Laurus nobilis L. by Danno G. Bay Laurel Contains Supercrital CO2. Molecules. 2008; 13: Antimutagenic Kaempferyl Coumarate 1702-1711. Acting against the Dietary Carcinogen 26. Yalcin H, Anik M, Sanda MA, Cakir A. 3-Amino-1-methyl-5H-pyrido [4, 3- Gas chromatography/mass b]indole. J. Agric. Food Chem. 1998; spectrometry analysis of Laurus nobilis 46 (12): 4864–4868. essential oil composition of northern 35. Bilen S, Bulut M. Effect of Laurel Cyprus. J Med Food. 2007; 10(4):715- (Laurus nobilis) on the non-Specific 719. immune responses of rainbow trout 27. Nayak S, Nalabothu P, Sandiford S, (Oncorhynchus mykiss, Walbaum). Bhogadi V, Adogwa A. Evaluation of Journal of Animal and Veterinary wound healing activity of Allamanda Advances.2010; 9(8):1275-1277. cathartica. L. and Laurus nobilis. L. 36. Loizzo MR, Saab AM, Tundis R, Statti extracts on rats. Complementary and GA, Menichini F, Lampronti I, Gambari Alternative Medicine. 2006; 6: 12. R, Cinatl J, Doerr HW. Phytochemical 28. Ham A, Shin J, Oh K, Lee S, Nam K, analysis and in vitro antiviral activities Koo U, Kim KH, Mar W. of the essential oils of seven Lebanon Neuroprotective Effect of the n- species. Chem Biodivers. Hexane Extracts of Laurus nobilis L. in 2008;5(3):461-470. Models of Parkinson’s Disease. 37. Ferreira A, Proenca C, Serralheiro Biomol Ther. 2011; 19(1): 118-125. MLM, Araujo MEM. The in vitro 29. Elmastas M, Gulcin I, Isildak O, screening for acetylcholinesterase Kufrevioglu OI, Ibaoglu K, Aboul-Enein inhibition and antioxidant activity of HY. Radical Scavenging Activity and medicinal plants from . Antioxidant Capacity of Bay Leaf Journal of Ethnopharmacology. 2006; Extracts. Journal of the Iranian 108(1): 31-37. Chemical Society. 2006;3(3): 258-266. 38. Erler F, Ulug I, Yalcinkaya B, Repellent activity of five essential oils

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against Culex pipiens. Fitoterapia. 2006; 77 (7-8): 491-494. 39. Ozcan B, Esen M, Sangun M, Coleri A, Caliskan M. Effective antibacterial and antioxidant properties of methanolic extract of Laurus nobilis seed oil. Journal of Environmental Biology. 2010; 31(5): 637-64. 40. Corato DU, Trupo M, Leone GP, Sanzo DG, Zingarelli G, Adami M. Antifungal activity of the leaf extracts of laurel (Laurus nobilis L.), orange (Citrus sinensis Osbeck) and olive (Olea europaea L.) obtained by means of supercritical carbon dioxide technique. Journal of Plant Pathology. 2007; 89 (3): 83-91. 41. Macchioni F, Perrucci S, Cioni P, Morelli I, Castilho P, Cecchi F. Composition and acaricidal activity of Laurus novocanariensis and Laurus nobilis essential oils against Psoroptes cuniculi. Journal of Essential Oil Research. 2006; 18: 111-114.

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