Full Plastome Sequence of the Fern Vandenboschia Speciosa (Hymenophyllales): Structural Singularities and Evolutionary Insights

Journal of Plant Research https://doi.org/10.1007/s10265-018-1077-y

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Full plastome sequence of the fern Vandenboschia speciosa (Hymenophyllales): structural singularities and evolutionary insights

F. J. Ruiz‑Ruano1 · B. Navarro‑Domínguez1 · J. P. M. Camacho1 · Manuel A. Garrido‑Ramos1

Received: 9 July 2018 / Accepted: 26 November 2018 © The Botanical Society of Japan and Springer Japan KK, part of Springer Nature 2018

Abstract We provide here the first full chloroplast genome sequence, i.e., the plastome, for a species belonging to the fern order Hymenophyllales. The phylogenetic position of this order within leptosporangiate ferns, together with the general scarcity of information about fern plastomes, places this research as a valuable study on the analysis of the diversity of plastomes throughout fern evolution. Gene content of V. speciosa plastome was similar to that in most ferns, although there were some characteristic gene losses and lineage-specific differences. In addition, an important number of genes required U to C RNA editing for proper protein translation and two genes showed start codons alternative to the canonical AUG (AUA). Concern- ing gene order, V. speciosa shared the specific 30-kb inversion of euphyllophytes plastomes and the 3.3-kb inversion of fern plastomes, keeping the ancestral gene order shared by eusporangiate and early leptosporangiate ferns. Conversely, V. speciosa has expanded IR regions comprising the rps7, rps12, ndhB and trnL genes in addition to rRNA and other tRNA genes, a condition shared with several eusporangiate ferns, lycophytes and hornworts, as well as most seed plants.

Keywords Ferns · Gene losses · IR expansion · Non-canonical start codons · Plastome · RNA editing · Vandenboschia speciosa

Introduction composed of genes related with their function in the eukary- otic cell, so that the plastome contains genes that encode The era of genomics allows not only the untangling of the for proteins involved in photosynthesis and for proteins and most recondite details of nuclear genome composition in RNAs involved in gene expression. eukaryotic cells, but also facilitates the study of the genomes Plastomes of land plants are generally conserved in struc- of cytoplasmic organelles in a relatively simple and time- ture, gene content and gene order (Gao et al. 2010; Green saving way. Eukaryotic cells contain mitochondria respon- 2011; Ruhlman and Jansen 2014; Wicke et al. 2011; Wolf sible for cellular respiration and cellular metabolism regu- et al. 2010; Wolf and Karol 2012; Xu et al. 2015). Most lation. Specifically, plants are characterized by also having plastomes are characterized by a quadripartite structure, chloroplasts that allow plants to convert light energy into including two copies of an inverted repeat (IRA and IRB) chemical energy through photosynthesis. Both organelles and the large (LSC), and small (SSC) single copy regions. are prokaryotic endosymbionts in origin and both contain The plastome usually includes 120–130 genes and varies in their own genomes, which have been drastically reduced size from 120 to 170 kilobases (kb). However, specific line- during evolution. Current organelle genomes are basically ages of land plants are characterized by gene and/or intron losses or duplications changing gene content in the plastome as well as some rearrangements changing its gene order Electronic supplementary material The online version of this (Gao et al. 2010; Green 2011; Ruhlman and Jansen 2014; article (https​://doi.org/10.1007/s1026​5-018-1077-y) contains Wicke et al. 2011; Wolf et al. 2010; Wolf and Karol 2012; supplementary material, which is available to authorized users. Xu et al. 2015). These changes appear to be phylogenetically * Manuel A. Garrido‑Ramos informative (Gao et al. 2009, 2011, 2013; Grewe et al. 2013; [email protected] Karol et al. 2010; Kim et al. 2014; Logacheva et al. 2017; Wolf et al. 2010; Zhu et al. 2016) and some examples are 1 Departamento de Genética, Facultad de Ciencias, known in bryophytes (e.g. Park et al. 2018; Wolf and Karol Universidad de Granada, Granada, Spain

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2012), lycophytes (e.g. Guo et al. 2016; Tsuji et al. 2007; Wolf et al. 2005), monilophytes (e.g., Gao et al. 2009, 2011, 2013; Grewe et al. 2013; Karol et al. 2010; Kim et al. 2014; Logacheva et al. 2017; Lu et al. 2015; Roper et al. 2007; Wolf et al. 2003, 2010; Zhong et al. 2014; Zhu et al. 2016), and seed plants (in both gymnosperm and angiosperm line- ages) (e.g. Green 2011; Ruhlman and Jansen 2014; Sun et al. 2016; Wicke et al. 2011; Xu et al. 2015; Zhu et al. 2016). Plastome sequence is thus a useful tool to build phylog- enies which have contributed very much to our understand- ing of plant evolution. It is thus essential to analyze plastome diversity across all plant lineages, and this is especially cru- cial in understudied groups such as ferns (Gao et al. 2013; Grewe et al. 2013; Karol et al. 2010; Kim et al. 2014; Labiak and Karol 2017; Roper et al. 2007; Wolf et al. 2010). Extant ferns are a lineage of non-seed vascular plants including 45 families and about 280 genera (Knie et al. 2015; Lehtonen 2011, PPG I 2016; Qi et al. 2018; Rai and Graham 2010; Rothfels et al. 2015; Schuettpelz and Pryer 2007). Molecu- lar phylogenies have revealed a basal dichotomy within the vascular plants (Pryer et al. 2001), separating the lyco- phytes (less than 1% of the current vascular plants) from the euphilophytes, among which there are two major clades, Fig. 1 Schematic representation of currently accepted ordinal fern the monilophytes or ferns and the spermatophytes or seed phylogeny (PPG I 2016) derived from the best available data (mainly from Knie et al. 2015; Lehtonen 2011; Qi et al. 2018; Rai and Gra- plants. Monilophyte includes the classes Psilotopsida (orders ham 2010; Rothfels et al. 2015; Schuettpelz and Pryer 2007) Psilotales and Ophioglossales), Equisetopsida (order Equi- setales), Marattiopsida (order Marattiales) and Polypodi- opsida (Fig. 1). The class Polypodiopsida, also known as (Lu et al. 2015). This clearly indicates that the diversity of leptosporangiate ferns (sporangia arise from a single epi- plastome structures in ferns has insufficiently been explored, dermal cell and not from a group of cells as in eusporangi- precluding a comprehensive study of plastome evolution ate ferns), includes the vast majority of extant ferns, with in this group (Grewe et al. 2013; Logacheva et al. 2017). more than 80% of about 10,500 fern species. Conversely, Furthermore, among complete plastome sequences, there the classes Psilotopsida, Equisetopsida and Marattiopsida are no representatives from the order Hymenophyllales, comprise the grade of eusporangiate ferns. The monophy- a key lineage within non-core, early leptosporangiates. letic group of leptosporangiates is composed of a total of Thus, the recent phyloplastomic analysis of ferns carried seven orders (Knie et al. 2015; Lehtonen 2011, PPG I 2016; out by Lu et al. (2015) included at least one representa- Qi et al. 2018; Rai and Graham 2010; Rothfels et al. 2015; tive species of each Monilophyte order except for the order Schuettpelz and Pryer 2007). Three leptosporangiate orders, Hymenophyllales. Salviniales (heterosporous ferns), Cyatheales (tree ferns), Our aim here is to fill this gap by analyzing the full and Polypodiales (polypods), form the large monophyletic sequence of the plastome of Vandenboschia speciosa clade of core leptosporangiates (Smith et al. 2006). The rest (Hymenophyllaceae, Hymenophyllales). The family Hyme- of leptosporangiate species are included within the orders nophyllaceae, with about 600 species, has its origin in the Osmundales, Hymenophyllales, Gleicheniales and Schizae- Triassic and its highest degree of diversification took place ales (Fig. 1). about 160 mya during the Jurassic (Pryer et al. 2004). This Among the nearly 2,000 complete plastome sequences family is characterized by the “filmy” aspect of its sporo- available in the GenBank database, only less than 50 belong phytes (fronds constituted by a translucent sheet with a sin- to monilophytes (Kim et al. 2014; Logacheva et al. 2017; Lu gle layer of cells). Although this family shows pantropical et al. 2015; Zhu et al. 2016; our own look-over to the NCBI distribution, with high diversity in morphology and habitat web page https​://www.ncbi.nlm.nih.gov/genom​e/brows​e#!/ occupation (Dubuisson et al. 2003; Ebihara et al. 2007), V. organ​elles​/). In addition, the information about fern plasto- speciosa constitutes a rare Macaronesian-European end- mes is limited to a few representatives of most, but not all, emism, as it is the only representative in the area and one fern orders. And, within the different orders, only a few rep- of the most vulnerable fern species in Europe, restricted to resentative species have been analyzed from some families places considered as refuges of tertiary flora. The two phases

1 3 Journal of Plant Research of its life cycle are perennial and capable of reproducing by of this sequence with other plant plastomes performing a vegetative propagation (Rumsey et al. 1999). The sporophyte BLASTN (Altschul et al. 1990) search against the NCBI’s is rhizomatous and able to spread by fragmentation of its NR database. Once homology was confirmed with plasto- rhizome. The gametophyte is very different from the typi- mes, we used this partial sequence as a seed to assembly cal heart-shaped prothallus, it is characterized by being epi- the remaining sequence of the V. speciosa plastome using a geous and narrowly talose or filamentous (to such an extent random selection of 2 × 2,000,000 reads with the MITObim that it is often confused with the protonema of a bryophyte), v1.8 software (Hahn et al. 2013) with the “—quick” option. and produces geminiferous buds. All these characteristics A single run of MITObim was unable to assembly the full add valuable scientific interest to the selection of this species sequence, for which reason we used the assembled sequence as representative of the order Hymenophyllales for plastome for a new run with this same software in order to lengthen sequencing. the assembly. We ran MITObim four times to complete the full sequence. We assessed the quality of the resulting assembly by map- Materials and methods ping the Illumina reads on it with the Bowtie2 (Langmead and Salzberg 2012) software considering only read pairs Materials completely mapped (—no-mixed) and maximum insert size of 1,000 nt (—maxins 1,000). Then, we plotted the coverage Vandenboschia speciosa specimens were collected at the along the whole plastome using the output of Pysamstats Canuto de Ojén-Quesada (OJEN) population located in the (https​://githu​b.com/alima​nfoo/pysam​stats​) and manually Alcornocales Natural Park (Cádiz, Spain), geographical checked the uniformity of the mappings and the presence of coordinates: N36.127°/W5.585° (for voucher reference, see complete reads in truncated genes using IGV (Thorvaldsdót- Ben-Menni Schuler et al. 2017). Sporophytes were frozen tir et al. 2013), a visualization tool for genome-scale datasets in liquid nitrogen in the field and were stored at − 80 °C. (Fig. S1). Genomic DNA (gDNA) was isolated from five individuals of this population using the DNeasy plant Mini kit (Quia- gen). A pool of DNA was generated from this five specimen Annotation and related studies DNAs and Next Generation Sequencing of the pool was car- ried out based on the Illumina HiSeq 2000 PE 2 × 101 nt Annotation of the V. speciosa plastome was performed using yielding about 16 Gb data. 16 Gb represent a ~ 1.5× coverage a combination of GeSeq-Annotation of Organellar Genomes of the nuclear genome (10.496 Gb; Obermayer et al. 2002) (Tillich et al. 2017) and DOGMA (Dual Organellar GenoMe and ~ 923× coverage of the chloroplast genome (146.874 bp Annotator) (Wyman et al. 2004). From this initial annota- and 0.85% of the Illumina reads; see also Figure S1). Illu- tion, putative starts, stops, and intron positions were deter- mina sequencing data can be accessed at Short Read Archive mined by comparisons with homologous genes in other plas- (SRA) Genbank database in the BioProject PRJNA387541 tomes and by considering the possibility of RNA editing, under the accession number SRX2844191. which can modify the start and stop positions. tRNA genes were annotated using GeSeq, DOGMA, tRNAscan-SE v2.0 Genome assembly (Lowe and Chan 2016) and ARAGORN (Laslett and Can- back 2004). The circular gene map of the V. speciosa plas- The absence of the complete sequence of a plastome in the tome was drawn by OGDRAW (Lohse et al. 2013) located at GenBank for a relative species, except two partial sequences the GeSeq plataform (https​://chlor​obox.mpimp​-golm.mpg. belonging to Vandenboschia radicans of about 16 kb (Wolf de/index​.html) and subsequent minor manual modification et al. 2010; accession number HM021800) and 9 kb (Gao to indicate the quadripartite structure of the plastome. et al. 2011; accession number HQ658104), lead us to get Plastome structure, gene content, and other general char- a reference sequence of V. speciosa to be used as a seed acteristics were then compared with the published fern plas- in later steps. For this, we performed a clustering of Illu- tomes available in the NCBI website (http://www.ncbi.nlm. mina reads and a de novo assembly of repetitive elements nih. gov/) and in the literature. by means of the RepeatExplorer (RE) pipeline (Novák The EMBOSS suite of bioinformatics tools (Rice et al. et al. 2013). This software groups similar reads in clusters, 2000) was used for the detection of short internal repeats assembles the reads of each cluster to generate contigs for (direct or inverted), as well as palindromes, in the intergenic repetitive elements, and annotates the contigs. We ran RE spacer sequences, using the programs MATCHER, ETAN- with 2 × 500,000 V. speciosa gDNA Illumina reads and then DEM, EINVERTED, POLYDOT and PALINDROME. selected the longest contigs showing the highest number of The annotated plastome of V. speciosa was deposited in reads annotated as “plastid”. We then checked the homology GenBank under accession number MH648610.

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Results and discussion consecutive MITObim runs, we obtained the full plastome sequence. Bowtie2 mapping yielded a uniform profile Assembly and genome structure across all the sequence. The plastome of V. speciosa consists of 146,874 bp (Fig. The RE analysis clusterized 49% of the Illumina reads S2) and shows a typical quadripartite structure including a in 538 clusters, eight of which were annotated as chlo- large single-copy (LSC) region of 89,620 bp and a small sin- roplast DNA sequences. We selected the cluster no. 217, gle-copy (SSC) region of 21,398 bp, separated by a pair of composed of 815 reads and its longest contig with 4,626 identical inverted repeats (IRA and IRB) of 17,928 bp each nt to be used as a seed for MITObim assembly. After four (Fig. 2). The overall structure of the V. speciosa plastome is typical of vascular plants although several rearrangements

Fig. 2 Gene map of the V. speciosa plastome. Boxes on the inside the GC content represented by dark gray bars. The location of the IR and outside of the outer circle represent genes transcribed clock- and SC regions is marked on the inner circle. The location of the IRs wise and anti-clockwise, respectively. Gene boxes are color coded is marked also on the outer cycle by thick black lines. (Color figure by functional groups as shown in the key. The inner circle displays online)

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Table 1 Main features and GenBank accession numbers of plastomes from different bryophyte, lycophyte, monylophyte and seed plants species Accession number Size (bp) LSC (bp) SSC (bp) IRs (bp) %GC Genes Protein-coding tRNAs rRNAs

Angiosperms Arabidopsis thaliana NC_000932.1 154,478 84,170 17,780 26,264 36.30 113 79 30 4 Asparagus officinalis NC_034777.1 156,699 84,999 18,638 26,531 37.76 112 78 30 4 Glycine max NC_007942.1 152,218 83,175 17,895 25,574 34.00 111 77 30 4 Erodium carvifoliuma NC_015083.1 116,935 – – – 39.00 108 76 28 4 Gymnosperms Cycas taitungensis NC_009618.1 163,403 90,216 23,039 25,074 39.50 118 83 31 4 Ginkgo biloba NC_016986.1 156,945 99,221 22,258 17,733 39.60 120 81 35 4 Welwitschia mirabilis NC_010654.1 119,726 68,556 11,156 20,007 38.00 101 66 31 4 Pinus bungeanab NC_028421.1 117,861 65,373 51,538 475 38.80 111 71 36 4 Polypodiales Pteridium aquilinum NC_014348.1 152,362 84,335 21,259 23,384 41.53 117 84 29 4 Adiantum capillus-veneris NC_004766.1 150,568 82,282 21,392 23,447 42.01 117 84 29 4 Cyatheales Alsophila spinulosa NC_012818.1 156,661 86,308 21,623 24,365 40.43 117 85 28 4 Salviniales Marsilea crenata NC_022137.1 151,628 87,828 22,210 20,795 42.22 117 85 28 4 Schizaeales Lygodium japonicum NC_022136.1 157,260 85,448 21,652 25,080 40.64 118 86 28 4 Gleicheniales Diplopterygium glaucum NC_024158.1 151,007 99,857 21,982 14,584 40.16 119 84 30 4 Hymenophyllales Vandenboschia speciosa MH648610 146,874 89,620 21,398 17,928 37.50 120 85 31 4 Osmundales Osmunda cinnamomea NC_024157.1 142,812 100,294 22,300 10,109 40.23 121 84 33 4 Marattiales Angiopteris evecta NC_008829.1 153,901 89,709 22,086 21,053 35.48 121 85 32 4 Psilotales Psilotum nudum NC_003386.1 138,829 84,617 16,304 18,954 36.03 118 81 33 4 Ophioglossales Ophioglossum californicum NC_020147.1 138,270 99,058 19,662 9775 42.15 120 84 32 4 Equisetales Equisetum arvense NC_014699.1 133,309 93,542 19,469 10,149 33.36 121 84 33 4 Equisetum hyemale NC_020146.1 131,760 92,580 18,994 10,093 33.74 121 84 33 4 Lycophytes Isoetes flaccida NC_014675.1 145,303 91,862 27,205 13,118 37.94 120 84 32 4 Huperzia lucidula NC_006861.1 154,373 104,088 19,657 15,314 36.30 119 86 29 4 Hornworts Anthoceros formosae NC_004543.1 161,162 107,503 22,171 15,744 32.90 120 84 32 4 Mosses Physcomitrella patens NC_005087.1 122,890 85,211 18,501 9589 28.50 119 84 31 4 Liverworts Marchantia polymorpha NC_001319.1 121,024 81,095 19,813 10,058 28.80 124 89 31 4 a This species has lost the IRs b This species has residual IRs were detected (see below). Table 1 compares the structure plastomes have lengths ranging between 131.760 bp (Equi- of V. speciosa plastome with that of different fern species, as setum hyemale) and 157.260 bp (Lygodium japonicum), well as those of lycophytes and bryophytes. Sequenced fern similar to other land plant groups (Table 1). Specifically,

1 3 Journal of Plant Research within this range, the V. speciosa plastome shows an inter- rrn23, all of them being duplicated within the IR regions), mediate size. 31 tRNAs genes (six of them being duplicated within the Plastome size is a feature that depends on lineage-spe- IR regions) and 85 protein-coding genes, three of which cific expansions and contractions (see Table 1). More than are duplicated within the IR regions and four are truncated half of the V. speciosa plastome is composed of non-coding at the 5′ end suggesting a process of pseudogenization regions (79,835 bp; 55.36%) including introns and intergenic (Table 2). As mentioned above, Bowtie2 mapping yielded sequences, and has an overall G + C content of 37.5%, simi- a uniform profile across all the sequence and we did not lar to other vascular plant plastomes, but higher than that in find truncated reads in regions comprising these four non-vascular land plant plastomes (Table 1) (reviewed in genes, meaning that their truncations were not assembling Park et al. 2018). As in other cases (for example, see Gao artifacts (Fig. S1). There are 17 intron-containing genes, et al. 2009), the G + C content is unevenly distributed across including six tRNA genes and 11 protein-coding genes, the plastome depending on location and functional group summing up 21 introns since three genes have more than (Fig. 2): 54.76% in rRNA genes, 53.62% in tRNA genes, one intron (Table 2). rps12 is a trans-spliced gene showing 38.40% in protein coding regions and 34.46% in intergenic two exons, one located in the LSC region (3′ end) and the spacers. other placed, in two copies, within the IRs (5′ end). From an evolutionary point of view, V. speciosa plas- Gene content tome shows a gene content that is characteristic of early leptosporangiate ferns (Kim et al. 2014). Notwithstanding, Table 2 lists the genes found in the plastome of V. spe- there were a few singularities in this species concerning ciosa, a repertoire of genes typical of land plant plastomes. some tRNA and protein-coding genes (Fig. 3) examined We identified four rRNAs genes (rrn4.5, rrn5, rrn16 and in detail in the following subsections.

Table 2 Catalogue of genes Gene class Genes identified in the plastome of V. 1 speciosa classified according to ATP synthase atpA atpB atpE atpF atpH atpI gene class Chlorophyll biosynthesis chlB chlL chlN Cytochrome petA petB petD petG petL petN 1 1 2 Hypothetical protein ycf1 * ycf2 * ycf3 ycf4 2 Miscellaneous proteins accD cemA ccsA clpP infA matK 1 1 NADH dehydrogenase ndhA ndhB ndhC ndhD ndhE ndhF ndhG ndhH ndhI ndhJ ndhK Photosystem I psaA psaB psaC psaI psaJ psaM Photosystem II psbA psbB psbC psbD psbE psbF psbH psbI psbJ psbK psbL psbM pbf1(psbN) psbT psbZ psb30 (ycf12) 1 3 RNA polymerase rpoA rpoB rpoC1 rpoC2 Rubisco rbcL 1 Ribosomal protein rpl2 rpl14 rpl16 rpl20 rpl21 rpl22* rpl23 rpl32 rpl33 rpl36 rps2 rps3 rps4 rps7 rps8 rps11 rps12 rps14 rps15 rps161* rps18 rps19 1 tRNA genes trnA-UGC​ trnC-GCA​ trnD-GUC​ trnE-UUC​ trnF-GAA​ trnG-GCC​ trnG-UCC​1 trnH-GUG​ trnI-CAU​ trnI-GAU​1 trnL-CAA1​ trnL-CAG​ trnL-UAG​ trnfM-CAU​ trnM-CAU​ trnN-GUU​ trnP-GGG​ trnP-UGG​ trnQ-UUG​ trnR-ACG​ trnR-ACG​1 trnR-CCG​ trnR-UCU​ trnS-GCU​ trnS-GGA​ trnS-UGA​ trnT-GGU​ trnV-GAC​ trnV-UAC​1 trnW-CCA​ trnY-GUA​ rRNA genes rrn23 rrn16 rrn5 rrn4.5

Superscript numbers indicate the number of introns. Asterisk indicates that the gene is truncated at the 5′ end

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Fig. 3 Presence (+) or absence (−) of different protein-coding and rangiate ferns while green lines include leptosporangiate ferns (darker tRNA genes in the plastomes of representative species of each of the for core leptospornagiate ferns). (Color figure online) 11 fern orders. Ψ = pseudogene. Blue labeled lines include euspo- tRNA genes loss of its co-evolved group II intron splicing (Duffy et al. 2009; Hausner et al. 2006). The set of 31 tRNAs genes of the V. speciosa plastome is In addition, V. speciosa plastome lacks two more tRNA assumed to be sufficient for the translation of chloroplast genes (trnS-CGA and trnT-UGU) which are usually present mRNAs (Gao et al. 2009). However, it lacks the tRNA gene in the plastomes of other land plants (Fig. 3). The loss of for lysine (trnK). Thus, although chloroplast tRNAs could trnS-CGA is shared with other leptosporangiates except, read most codons by using two-out-of-three and wobble again, with Osmunda cinnamomea which still preserves mechanisms (Gao et al. 2009; Pfitzinger et al. 1990), the this gene (Gao et al. 2013; Grewe et al. 2013; Kim et al. absence of trnK genes for reading any of the two lysine 2014). The trnT-UGU gene was also lost independently in codons suggests that cytosolic tRNAs may be imported into the eusporangiate Ophioglossum californicum (Gao et al. chloroplasts, despite a lack of experimental evidence (Gao 2013; Grewe et al. 2013), but not in other eusporangiates et al. 2009). The trnK-UUU gene of land plants has a large such as Equisetum, Psilotum or Angiopteris (Grewe et al. intron in which is nested the matK gene, a gene that encodes 2013; Karol et al. 2010; Roper et al. 2007). the protein maturase involved in splicing type II introns (Kuo Another specific feature is that the trnL-CAA gene, et al. 2011; Wicke and Quandt 2009). This structure is con- placed close to the ndhB gene, has mutated in V. speciosa served in eusporangiate ferns as well as in the leptospo- to trnL-CAG while, in most leptosporangiate ferns, it has rangiate Osmunda cinnamomea plastome (Osmundaceae, been lost, although it is still conserved in Gleicheniales Osmundales). However, the trnK-UUU gene was lost in V. (Grewe et al. 2013; Kim et al. 2014). Conversely, the trnL- speciosa whereas the matK gene was still maintained, like- UAA gene, placed between the rps4 and the ndhJ genes, has wise in the remaining leptosporangiate ferns. This supports mutated to trnL-CAA after the divergence of Osmundales the loss of trnK-UUU after the divergence of Osmundales (Kim et al. 2014), including V. speciosa (this paper). (Grewe et al. 2013; Kuo et al. 2011) (Fig. 3), but not the loss Figure 3 shows that the loss of the trnV-GAC gene of the intron-encoded matK. In plants, matK preferentially occurred in the common ancestor of core leptosporangiates catalyzes splicing of the trnK intron but, in ferns, it may and the Schizaeales (Grewe et al. 2013; Kim et al. 2014; also have maintained an ancient and generalized function Labiak and Karol 2017) but not in the rest of leptosporangi- as a maturase that catalyzes the splicing reactions of other ates represented by V. speciosa. On the other hand, the trnR- group II introns in the chloroplast genome, even after the CCG gene is preserved in all leptosporangiate ferns but has

1 3 Journal of Plant Research undergone several sequential anticodon changes, in some be missing or pseudogenized in distinct land plant lineages species, leading to alternative anticodons, pseudogenes or (Ruhlman and Jansen 2014). The ycf66 gene has been lost even trnR-UCA genes (Grewe et al. 2013; Wolf et al. 2003, in many fern species (Gao et al. 2011), including V. speciosa 2004). It has been suggested that trnR-UCA might recognize (this paper) although it is present in the partial sequence of internal UGA stop codons acting as a failsafe mechanism to the plastome of V. radicans (Gao et al. 2011). In fact, we ensure that arginine is correctly inserted into the protein at have detected a residual fragment of this gene in the plas- any internal UGA codons that were not properly converted tome of V. speciosa. In addition, this fragment has stops by U-to-C RNA editing into CGA (Grewe et al. 2013). How- codons and several indels. Specifically, this gene has been ever, that is not the case for V. speciosa which has UGA lost or is pseudogenized at least four times in fern lineages stop codons interrupting 12 genes, but lacks the trnR-UCA Ophioglossales, Psilotales, Equisetales, and core leptospo- mutation (see “Non-canonical start codons and internal stop rangiates, suggesting that the occurrence of this gene is codons”). highly unstable and, probably, that this ORF is irrelevant for a hypothetical and, up to now, uncharacterized, protein. Protein‑coding genes Other lineage-specific protein-coding gene losses included in Fig. 3 were not found in V. speciosa: the psaM Protein-coding gene content of V. speciosa plastome is simi- gene is missing from all sequenced plastomes of the order lar to that of most ferns, which conserve about 86 of these Polypodiales (Gao et al. 2013; Grewe et al. 2013; Wolf et al. genes, although there are some lineage-specific differences 2003), and it was lost in parallel also from some species in (Fig. 3). In the case of V. speciosa, we have found the partial other lineages (Labiak and Karol 2017). Events of recurrent loss of the sequences of four protein-coding genes (ycf1, losses were also found for other genes such as the chl genes ycf2, rps16 and rpl22). We assume that these genes are (chlB, chlL and chlN), which were lost in Psilotum nodum going through a process of pseudogenization that probably (Psilotales) (Grewe et al. 2013) and Actinostachys pennula would lead to their complete loss. In fact, we have found a (Schizaeaceae, Schizaeales) (Labiak and Karol 2017), but few additional indels (short deletions of one or a few nucleo- also in some angiosperm plastomes (Chaw et al. 2004; tides) both in ycf1 and ycf2 genes although not in the other Jansen et al. 2007). two cases. These four genes have important roles in chloro- The ndhB gene merits here a specific commentary as it plast gene expression and function; therefore, it is likely that may be duplicated or not depending on whether it is located these functions are supplied by other means such as those within the IR or the LSC regions. Furthermore, according found in other species with missing plastid genes. to Gao et al. (2013), there are three types of ndhB genes in Thus, the rps16 has been lost in several lineages of ferns ferns: one copy of exon 1 plus one copy of exon 2 (1:1 type), such as Ophioglossales, Psilotales and Equisetales (euspo- one copy of exon 1 plus two copies of exon 2 (1:2 type), rangiate ferns) (Grewe et al. 2013; Kim et al. 2014) and it and two copies of exon 1 plus two copies of exon 2 (2:2 seems that it will be lost in one specific lineage of leptospo- type) (Gao et al. 2013). In heterosporous ferns (core lepto- rangiates (V. speciosa) (Fig. 3). Furthermore, several paral- sporangiates) ndhB is single copied and located in the LSC lel losses of the rps16 gene have occurred along land plant (1:1 type) (see Gao et al. 2013). However, the second exon phylogeny (Xu et al. 2015). Missing genes in specific plant is duplicated in the plastome IRs of the two other orders of lineages might have been relocated from the plastome to core leptosporangiates, the tree ferns and the polypod ferns the nuclear genome or, alternatively, these functions were (1:2 type; see Gao et al. 2013). ndhB gene resides in IRs supplied by other means (Gantt et al. 1991; Gao et al. 2009; in two out of the four orders comprising the eusporangiate Jansen et al. 2011; Keller et al. 2017; Park et al. 2018; Ruhl- ferns, the psilotales and the marattioids (2:2 type) (Gao et al. man and Jansen 2014). In the genus Lupinus (Leguminosae), 2013). In contrast, the ndhB is 1:1 type in ophioglossoids the functional role of this gene in the chloroplast has been and equisetales (Gao et al. 2013). Remarkably, the copy replaced by the nuclear rps16 gene (Keller et al. 2017; Ruhl- status of ndhB in the non-core leptosporangiate fern line- man and Jansen 2014). And it has been shown that the rpl22 ages is highly variable, as V. speciosa (Hymenophyllales) gene has been transferred to the nucleus in different flow- shows a 2:2 type ndhB gene (this study), while Osmunda ering plant lineages (Gantt et al. 1991; Jansen et al. 2011; cinnamomea (Osmundales) shows a 1:1 type, but Diplop- Ruhlman and Jansen 2014). terygium glaucum (Gleicheniales) and Lygodium japoni- The pseudogenization of the ycf1 gene has also been cum (Schizaeales) a 1:2 type (Gao et al. 2013). These data documented in Angiopteris evecta (Roper et al. 2007). support the existence of parallel changes among major fern Both ycf1 and ycf2 genes have been designated as essential lineages that have occurred in a region (IR) prone to rear- genes because their targeted disruption results in unstable rangements and expansions/contractions (see next section). mutant phenotypes (Drescher et al. 2000; Ruhlman and Recently, Song et al. (2018) have reported a novel chlo- Jansen 2014). However, both ycf1 and ycf2 genes appear to roplast protein-coding gene (ycf94) of probable functional

1 3 Journal of Plant Research significance in ferns and, potentially, bryophytes and lyco- Table 3 List of genes with stop and non-canonical start codons rps16 matK phytes. This ORF is located between the and the Gene Stop codon Start codon genes. We have not found evidence for the presence of this ORF in the intergenic sequence between rps16 and matK accD CGA to UGA/CAA to UAA​ genes in V. speciosa. atpB CAA to UAA​ atpH ACG​ Non‑canonical start codons and internal stop atpI CAA to UAA​ codons cemA CAA to UAA​ chlB CAA to UAA​ Examination of start and stop codons (internal and terminal) chlN CAA to UAA​ in the V. speciosa plastome resulted in 27 protein-coding clpP UAU to UAA​ genes with one (most of the 27 genes) or a few internal matK CGA to UGA​ stop codons that required U-to-C RNA editing to overcome ndhA CAA to UAA​ the premature end translation by the incorporation of the ndhF CGA to UGA​ appropriate amino acid (Table 3). U-to-C editing in the first ndhG ACG​ codon position has been shown to be involved in the repair ndhH ACG​ of internal stop codons to either arginine or glutamine (Guo petA CAA to UAA​ et al. 2015; Knie et al. 2016; Kugita et al. 2003; Sugiura petD AUA​ 2008; Wolf et al. 2004). In fact, in V. speciosa, the stop rpoA CAA to UAA​ codons came from mutations from CGA (arginine) to UGA rpoB CGA to UGA and CAA to UAA​ or from CAA (glutamine) to UAA, according to the results rpoC1 CGA to UGA​ of BLAST search among homologous genes from other fern rpoC2 CGA to UGA and CAA to UAA​ species. rpl2 CGA to UGA​ U-to-C editing appears to be restricted in occurrence in rpl20 CAA to UAA​ most land plants but, it has been found that, in some fern rpl23 CGA to UGA​ lineages, U-to-C editing even exceeds the canonical C-to-U rpl33 CGA to UGA​ editing (Knie et al. 2016). In contrast, the reverse process, rps2 CAA to UAA​ RNA editing by C-to-U conversions to reconstitute con- rps3 CGA to UGA​ served codon identities has been extensively documented in rps4 CAA to UAA​ ferns (Wolf et al. 2004) and is common in land plant chlo- rps7 CGA to UGA​ roplasts and mitochondria, except in some liverworts which rps8 CAA to UAA​ have secondarily lost this type of RNA editing (Guo et al. rps12 CAA to UAA​ 2015; Groth-Malonek et al. 2007; Knie et al. 2016; Rüdinger rps15 AUA​ et al. 2012; Sugiura 2008). rps18 CGA to UGA​ In addition to the safeguard role against premature stop- ycf4 CGA to UGA/CAA to UAA​ ping, RNA editing occasionally can create an AUG initiation codon from an ACG codon by C-to-U edit (Sugiura 2008; Wolf et al. 2004). We have found that the atpH, the ndhG and ndhH the genes of V. speciosa begin with ACG. But we First, euphyllophytes (ferns and seed plants) have differ- also found two other genes beginning with non-canonical entiated plastomes with respect to lycophytes and non vas- AUG start codon (AUA), specifically the petD and the rps15 cular plants (hornworts, mosses and liverworts) (Raubeson genes (Table 3). It is known that translation can initiate at and Jansen 1992). The rearrangement consists in a 30-kb codons other than AUG (Kearse and Wilusz 2017) and that inversion comprising the region between the ycf2 and the some organelle and prokaryote genomes use GUG and AUA psbM genes (Fig. 4). As expected, V. speciosa shares this as alternate start codons for some of these genes (Guo et al. syntenic rearranged region with the rest of ferns. 2015; Kuroda et al. 2007; Wolf et al. 2004). Second, monilophytes (ferns) have a unique rear- ranged region consisting in a 3.3-kb inversion known as Gene order the trnG/trnT inversion in which the genes between these two tRNA genes have the trnG-psbZ-trnS-psbC-psbD-trnT There are four key rearrangements that characterize the evo- gene order while in the rest of land plants, the order is the lution of fern plastomes and mark differences between fern inverse (Wolf et al. 2003, 2010). Also expectedly, V. spe- and the rest of land plants as well as among different fern ciosa has the trnG-psbZ-trnS-psbC-psbD-trnT gene order groups (Gao et al. 2011). characteristic of ferns (Fig. 4).

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Fig. 4 Linear gene map of V. speciosa plastome compared to those of Blue arrows indicate the inversion ends. Red arrows indicate a change a hornworth, Marchantia polymorpha (NC_001319.1), a seed plant, of location for the region comprising the trnD-trnY-trnE genes (that Arabidopsis thaliana (NC_000932.1), and a core leptosporangiate it is not inverted) as a consequence of the double inversion in the fern, Alsophila spinulosa (NC_012818.1). Numbers represent the two regions flanking it. A box stands out the rearranged IR regions inverted regions described in the text: between euphyllophytes and in Alsophila spinulosa (the box also includes the LSC region). Genes the rest of land plants (1); between ferns and the rest of land plants with introns are labeled with asterisks. (Color figure online) (2); and the inversion that characterizes core leptosportangiates (3).

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Third, according to gene order, the fern plastomes can Inverted repeat (IRs) region expansions be classified into two main types (Gao et al.2009 , 2011, 2013; Zhu et al. 2016) (Figs. 4, 5). One comprising the The presence of inverted regions (IRs) within the plasto- plastomes of taxa diversifying before the separation of the mes is a common feature in most plants with a few excep- Schizaeales, which shares the ancestral gene order (Wolf tions among seed plants (Guo et al. 2014; Guisinger et al. et al. 2010), and the other comprising the plastomes of 2011; Zhu et al. 2016). This region comprises mostly Schizaeales and of core leptosporangiates, which has a four genes for the four chloroplastidial ribosomal RNAs, derived gene order (Gao et al. 2013; Labiak and Karol several tRNA genes and, depending of the species, a few 2017; Wolf et al. 2010). The derived gene order consists protein-coding genes. Figure 5 shows an inference of the of highly rearranged inverted repeats (IRs) with the rRNA ancestral inverted repeat (IR) content during land plant genes arranged in reverse order in comparison to all other evolution, based on a previous proposal by Zhu et al. plants, generated by two partially overlapping inversions (2016), but with some modifications. Our table includes spanning LSC and IR regions (Hasebe and Iwatsuki 1990; not only the V. speciosa data but also the reordered IR and; Iwatsuki 1992; Wolf et al. 2003, 2010) (Fig. 5). An region of Polypodiopsida and that of Schizaeales. The additional difference between the ancestral and derived rearrangement consisted in a pair of partially overlapping gene order is the region comprised between the rpoB and inversions in the region of the inverted repeat that occurred psbZ, which includes the trnD inversion (Fig. 4). This in the common ancestor of Schizaeales and Polypodiop- characteristic gene order is exclusive of Polypodiopsida sida (most ferns) (Wolf et al. 2010). Figure 5 includes the (Gao et al. 2009). structure of these ferns in a separate frame because these lineage-specific changes comprising the inversion of the

Fig. 5 Inverted repeat (IR) gene content during land plant evolution. lines include leptosporangiate ferns (darker for core leptospornagiate Rearranged IR regions of Core Leptosporangiates and Schizaeales ferns). (Asterisk) There are some specific losses/gains in specific line- are represented below. Shaded cells indicate an inverse order of these ages some of which have been represented below. (Double asterisks) genes in this species due to an inversion. Genes in square brackets ndhB and trnT genes are pseudogenized in most species analyzed are those partially encoded in the IR. Pseudogenes are represented within this order, but not in Adiantum capillus-veneris. (Color figure by Ψ. Blue labeled lines include eusporangiate ferns while green online)

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Table 4 Features and sequences of short direct and inverted repeats found in intergenic spacers of the V. speciosa plastome Flanking genes Size Repeats Identity (%) Consensus

Direct repeats petN/psbM 6 12 70.8 ATT​TAT​ trnY-GUA/trnE-UUC​ 27 5 84.4 GAA​CGG​ATT​TAG​ AGT​CCG​TCT​ CCC​ATC​ Flanking genes Size Identity (%) Repeat

Inverted repeats ndhB/ trnL-CAG​ 25 88 GAT​TTA​CAA​ATT​GAC​TCA​GTA​AAA​G/CTA​AAT​GTA​TAA​CTA​AGT​CAT​ CTT​C trnL-CAA/trnF-GAA​ 27 100 CTG​AAA​CGA​ACC​CAT​TCA​AGG​AAT​CCA/GAC​TTT​GCT​TGG​GTA​AGT​ TCC​TTA​GGT​ trnM-CAU/atpE 21 100 AAA​ACT​TAT​TGG​ATG​CCA​TAT/ATA​TGG​CAT​CCA​ATA​AGT​TTT​ rpl14/rpl16 19 100 ATA​GGA​AGA​GAT​AGT​TCC​A/TAT​CCT​TCT​CTA​TCA​AGG​T ycf1/chlN 22 100 TAG​ATT​TAT​TAA​CCA​TAA​AGGA/ATC​TAA​ATA​ATT​GGT​ATT​TCCT​

IR gene order have no accommodation in the ancestral Repeat sequences in the plastome of V. speciosa reconstruction. Furthermore, the IRs of Polypodiales plas- tomes are dynamic, driven by such events as gene loss, Table 4 shows all the tandem and inverted short repeats duplication and putative lateral transfer from mitochondria found within the intergenic spacers of the V. speciosa plas- (Logacheva et al. 2017). tome. There are 2 direct repeats and 5 inverted repeats. As can be seen in Fig. 5, seed plants (both, gymno- The two direct repeats are interestingly localized in the sperms and angiosperms) show a derived condition appar- rpoB/psbZ region. Specifically, one of them (a 27-bp ently resulting from an expansion of the IRs that include not repeat) was previously described in V. radicans (Gao only rRNA and tRNA genes but also protein coding genes et al. 2011). Similar to V. speciosa (this study; see Fig. commonly found in the LSC region in ferns, lycophytes S2), V. radicans shows a 27-bp tandem repeat within the and bryophytes. Furthermore, there are some specific gains trnY-trnE intergenic spacer which shows strong sequence and/or losses in certain lineages. Major losses occurred in similarity with the anticodon domain of trnY, the tRNA Erodium and Medicago or in Pinaceae or Cupressophytes gene for tyrosine (Gao et al. 2011). While the 27-bp repeat where plastomes retains only residual inverted repeats or of V. radicans is repeated 17 times expanding the spacer lack IRs (reviewed in Zhu et al. 2016). Some lycophytes approximately threefold in length (Gao et al. 2011), this and fern lineages have also expanded IRs like seed plants, repeat appears only 5 times in the V. speciosa plastome although in a less extension. For example, this occurred in V. (this paper). These repeats have the potential to form stem- speciosa (this paper), but also in some eusporangiate ferns loop structures which point to a significant functional rel- as well as in some lycophytes and non vascular plants such evance and to play a major role in the expansion of the as hornworts. The length of the expansion depends of the trnY-trnE intergenic spacer (Gao et al. 2011). species. In V. speciosa for example, the expansion includes In addition, the plastome of V. speciosa contains one the rps7, rps12 and ndhB genes, which is the more com- more tandem direct repeat, being short (6 bp) and repeated mon situation in some eusporangiate ferns and in lycophytes 12 times in the petN-psbM intergenic spacer (also the as well as hornworts with expanded IRs. Interestingly, this rpoB/psbZ region). Extensive rearrangements in the plas- gene order and organization at IRs, define the plastome of tome of different species have been associated with the V. speciosa as one of the species that experienced the recur- concentration of direct repeats and tRNA genes (Chumley rent IR expansion. Notwithstanding, the possibility remains et al. 2006; Haberle et al. 2008; Guisinger et al. 2011). that what we have defined as parallel expansions of the IR In fact, the rpob/psbZ region is characterized by notable regions might be the result of a preservation of the ancestral structural rearrangements through fern evolution directly condition while recurrent contractions might explain the related with the accumulation of tRNA genes as well as by smallest inverted regions found in non seed plants. This is highly variable intergenic spacers in some lineages which also supported by the fact that the IRs of Polypodiales, even includes gene losses and intergenic expansions throughout reordered, also contains the protein-coding genes found in direct repeats (Gao et al. 2011). expanded IRs (Fig. 5).

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Additionally, the V. speciosa plastome shows five short Gantt JS, Baldauf SL, Calie PJ, Weeden NF, Palmer JD (1991) inverted repeats in five different intergenic spacers (Table 4). Transfer of rpl22 to the nucleus greatly preceded its loss from the chloroplast and involved the gain of an intron. EMBO J Pairwise comparisons between the two components of four 10:3073–3078 of the inverted repeats revealed 100% identity, while the Gao L, Yi X, Yang Y-X, Su Y-J, Wang T (2009) Complete chloroplast identity was 84% between the two components of the fifth genome sequence of a tree fern Alsophila spinulosa: insights into inverted repeat. Additionally, this latter inverted repeat is evolutionary changes in fern chloroplast genomes. BMC Evol Biol 9:130 duplicated because it is within the IR regions between the Gao L, Su Y-J, Wang T (2010) Plastid genome sequencing, compara- ndhB and the trnL-CAG genes. Inverted repeats have the tive genomics, and phylogenomics: current status and prospects. potential to form cruciform structures, which are funda- J Syst Evol 48:77–93 mentally important for a wide range of biological processes, Gao L, Zhou Y, Wang Z-W, Su Y-J, Wang T (2011) Evolution of the rpoB-psbZ region in fern plastid genomes: notable structural rear- including replication, regulation of gene expression, nucleo- rangements and highly variable intergenic spacers. BMC Plant some structure and recombination (Brázda et al. 2011) and Biol 11:64 might be involved in plastome rearrangements (Inagaki et al. Gao L, Wang B, Wang Z-W, Zhou Y, Su Y-J, Wang T (2013) Plastome Lygodium japonicum Marsilea crenata 2013; Kolb et al. 2009). sequences of and reveal the genome organization transformation from basal ferns to core Acknowledgements leptosporangiates. Genome Biol Evol 5:1403–1407 This research has been financed by the Spanish Green BR (2011) Chloroplast genomes of photosynthetic eukaryotes. Ministerio de Economía y Competitividad and FEDER founds, Grant: Plant J 66:34–44 CGL2010-14856 (subprograma BOS). The Dirección General de Grewe F, Guo W, Gubbels EA, Hansen AK, Mower JP (2013) Com- Gestión del Medio Natural y Espacios Protegidos of the Consejería de plete plastid genomes from Ophioglossum californicum, Psilo- Medio Ambiente y Ordenación del Territorio de la Junta de Andalucía tum nudum, and Equisetum hyemale reveal an ancestral land plant authorized and facilitates the sampling of the material. We are highly genome structure and resolve the position of Equisetales among indebted to Carmen Rodríguez Hiraldo and to Jaime Pereña Ortiz who, monilophytes. BMC Evol Biol 13:8 together with the team of Agentes de Medio Ambiente of the Conseje- Groth-Malonek M, Wahrmund U, Polsakiewicz M, Knoop V (2007) ría, helped us with the sampling procedure. Evolution of a pseudogene: exclusive survival of a functional mitochondrial nad7 gene supports Haplomitrium as the earliest liverwort lineage and proposes a secondary loss of RNA editing in Marchantiidae. Mol Biol Evol 24:1068–1074 References Guisinger MM, Kuehl JV, Boore JL, Jansen RK (2011) Extreme recon- figuration of plastid genomes in the angiosperm family Gerani- aceae: rearrangements, repeats, and codon usage. Mol Biol Evol Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic 28:583–600 local alignment search tool. J Mol Biol 215:403–410 Guo W, Grewe F, Cobo-Clark A, Fan W, Duan Z, Adams RP, Schwar- Ben-Menni Schuler SBM, García-López MC, López-Flores I, Nieto- zbach AE, Mower JP (2014) Predominant and substoichiometric Lugilde M, Suárez-Santiago VN (2017) Genetic diversity and isomers of the plastid genome coexist within Juniperus plants population history of the Killarney fern, Vandenboschia speciosa and have shifted multiple times during cupressophyte evolution. (Hymenophyllaceae), at its southern distribution limit in conti- Genome Biol Evol 6:580–590 nental Europe. Bot J Linn Soc 183:94–105 Guo W, Grewe F, Mower JP (2015) Variable frequency of plastid RNA Brázda V, Laister RC, Jagelská EB, Arrowsmith C (2011) Cruciform editing among ferns and repeated loss of uridine-to-cytidine edit- structures are a common DNA feature important for regulating ing from vascular plants. PLoS One 10:e0117075 biological processes. BMC Mol Biol 12:33 Guo Z-Y, Zhang H-R, Shrestha N, Zhang X-C (2016) Complete chlo- Chaw SM, Chang CC, Chen HL, Li WH (2004) Dating the monocot- roplast genome of a valuable medicinal plant, Huperzia serrata dicot divergence and the origin of core eudicots using whole chlo- (Lycopodiaceae), and comparison with its congener. Appl Plant roplast genomes. J Mol Evol 58:424–441 Sci 4:1600071 Chumley TW, Palmer JD, Mower JP, Fourcade HM, Calie PJ, Boore Haberle RC, Fourcade HM, Boore JL, Jansen RK (2008) Extensive JL, Jansen RK (2006) The complete chloroplast genome sequence rearrangements in the chloroplast genome of Trachelium caer- of Pelargonium × hortorum: organization and evolution of the uleum are associated with repeats and tRNA genes. J Mol Evol largest and most highly rearranged chloroplast genome of land 66:350–361 plants. Mol Biol Evol 23:2175–2190 Hahn C, Bachmann L, Chevreux B (2013) Reconstructing mitochon- Drescher A, Ruf S, Calsa T, Carrer H, Bock R (2000) The two largest drial genomes directly from genomic next-generation sequencing chloroplast genome encoded open reading frames of higher plants reads—a baiting and iterative mapping approach. Nucleic Acids are essential genes. Plant J 22:97–104 Res 41:e129 Dubuisson J-Y, Hennequin S, Douzery EJP, Cranfill RB, Smith AR, Hasebe M, Iwatsuki K (1990) Chloroplast DNA from Adiantum capil- Pryer KM (2003) rbcL phylogeny of the fern genus Trichomanes lus-veneris L., a fern species (Adiantaceae); clone bank, physical (Hymenophyllaceae), with special reference to neotropical taxa. map and unusual gene localization in comparison with angio- Int J Plant Sci 164:753–761 sperm chloroplast DNA. Curr Genet 17:359–364 Duffy AM, Kelchner SA, Wolf PG (2009) Conservation of selection Hasebe M, Iwatsuki K (1992) Gene localization on the chloroplast on matK following an ancient loss of its flanking intron. Gene DNA of the maiden hair fern, Adiantum capillus-veneris. J Plant 438:17–25 Res 105:413–419 Ebihara A, Iwatsuki K, Ito M, Hennequin S, Dubuisson J-Y (2007) Hausner G, Olson R, Simon D, Johnson I, Sanders ER, Karol KG, A global molecular phylogeny of the fern genus Trichomanes McCourt RM, Zimmerly S (2006) Origin and evolution of the (Hymenophyllaceae) with special reference to stem anatomy. Bot chloroplast trnK (matK) intron: a model for evolution of group II J Linnean Society 155:1–27 intron RNA structures. Mol Biol Evol 23:380–391

1 3 Journal of Plant Research

Inagaki H, Ohye T, Kogo H, Tsutsumi M, Kato T, Tong M, Emanuel Lohse M, Drechsel O, Kahlau S, Bock R (2013) OrganellarGenome- BS, Kurahashi H (2013) Two sequential cleavage reactions on cru- DRAW—a suite of tools for generating physical maps of plastid ciform DNA structures cause palindrome-mediated chromosomal and mitochondrial genomes and visualizing expression data sets. translocations. Nature Commun 4:1592 Nucleic Acids Res 41:W575–W581 Jansen RK, Cai Z, Raubeson LA et al (2007) Analysis of 81 genes Lowe TM, Chan PP (2016) tRNAscan-SE on-line: search and con- from 64 plastid genomes resolves relationships in angiosperms textual analysis of transfer RNA genes. Nucleic Acids Res and identifies genome-scale evolutionary patterns. Proc Natl Acad 44:W54–W57 Sci USA 104:19369–19374 Lu J-M, Zhang N, Du X-Y, Wen J, Li D-Z (2015) Chloroplast phy- Jansen RK, Saski C, Lee S, Hansen AK, Daniell H (2011) Complete logenomics resolves key relationships in ferns. J Syst Evol plastid genome sequences of three rosids (Castanea, Prunus, 53:448–457 Theobroma): evidence for at least two independent transfers of Novák P, Neumann P, Pech J, Steinhaisl J, Macas J (2013) Repeat- rpl22 to the nucleus. Mol Biol Evol 28:835–847 Explorer: a galaxy-based web server for genome-wide charac- Karol KG, Arumuganathan K, Boore JL, Duffy AM, Everett KDE, Hall terization of eukaryotic repetitive elements from next-generation JD, Hansen SK, Kuehl JV, Mandoli DF, Mishler BD, Olmstead sequence reads. Bioinformatics 29:792–793 RG, Renzaglia KS, Wolf PG (2010) Complete plastome sequences Obermayer R, Leitch IJ, Hanson L, Bennett MD (2002) Nuclear DNA of Equisetum arvense and Isoetes flaccida: implications for phy- C-values in 30 species double the familial representation in pteri- logeny and plastid genome evolution of early land plant lineages. dophytes. Ann Bot 90:209–217 BMC Evol Biol 10:321 Park M, Park H, Lee H, Lee B, Lee J (2018) The complete plastome Kearse MG, Wilusz JE (2017) Non-AUG translation: a new start for sequence of an Antarctic bryophyte Sanionia uncinata (Hedw.) protein synthesis in eukaryotes. Genes Dev 31:1717–1731 Loeske. Int J Mol Sci 19:709 Keller J, Rousseau-Gueutin M, Martin GE, Morice J, Boutte J, Cois- Pfitzinger H, Weil JH, Pillay DT, Guillemaut P (1990) Codon recogni- sac E, Ourari M, Aïnouche M, Salmon A, Cabello-Hurtado F, tion mechanisms in plant chloroplasts. Plant Mol Biol 14:805–814 Aïnouche A (2017) The evolutionary fate of the chloroplast and PPG I (2016) A community-derived classification for extant lycophytes nuclear rps16 genes as revealed through the sequencing and com- and ferns. J Syst Evol 54:563–603 parative analyses of four novel legume chloroplast genomes from Pryer KM, Schneider H, Smith AR, Cranfill R, Wolf PG, Hunt JS, Lupinus. DNA Res 24:343–358 Sipes SD (2001) Horsetails and ferns are a monophyletic group Kim HT, Chung MG, Kim K-J (2014) Chloroplast genome evolution and the closest living relatives to seed plants. Nature 409:618–622 in early diverged leptosporangiate ferns. Mol Cells 37:372–382 Pryer KM, Schuettpelz E, Wolf PG, Schneider H, Smith AR, Cran- Knie N, Fischer S, Grewe F, Polsakiewicz M, Knoop V (2015) Horse- fill R (2004) Phylogeny and evolution of ferns (monilophytes) tails are the sister group to all other monilophytes and Marattiales with a focus on the early leptosporangiate divergences. Am J Bot are sister to leptosporangiate ferns. Mol Phyl Evol 90:140–149 91:1582–1598 Knie N, Grewe F, Fischer S, Knoop V (2016) Reverse U-to-C editing Qi X, Kuo L-Y, Guo C, Li H, Li Z et al (2018) A well-resolved fern exceeds C-to-U RNA editing in some ferns—a monilophyte-wide nuclear phylogeny reveals the evolution history of numerous tran- comparison of chloroplast and mitochondrial RNA editing sug- scription factor families. Mol Phylogenet Evol 127:961–977 gests independent evolution of the two processes in both orga- Rai HS, Graham SW (2010) Utility of a large, multigene plastid data nelles. BMC Evol Biol 16:134 set in inferring higher-order relationships in ferns and relatives Kolb J, Chuzhanova NA, Högel J, Vasquez KM, Cooper DN, Bacolla (monilophytes). Am J Bot 97:1444–1456 A, Kehrer-Sawatzki H (2009) Cruciform-forming inverted repeats Raubeson LA, Jansen RK (1992) Chloroplast genomes of plants. In: appear to have mediated many of the microinversions that dis- Henry RJ (ed) Plant diversity and evolution: genotypic and phe- tinguish the human and chimpanzee genomes. Chromosome Res notypic variation in higher plants. CABI Publishing, London, 17:469–483 pp 45–68 Kugita M, Yamamoto Y, Fujikawa T, Matsumoto T, Yoshinaga K Rice P, Longden I, Bleasby A (2000) EMBOSS: the european molecu- (2003) RNA editing in hornwort chloroplasts makes more than lar biology open software suite. Trends Genet 16:276–277 half the genes functional. Nucleic Acids Res 31:2417–2423 Roper JM, Hansen SK, Wolf PG, Karol KG, Mandoli DF, Everett KDE, Kuo LY, Li FW, Chiou WL, Wang CN (2011) First insights into fern Kuehl J, Boore JL (2007) The complete plastid genome sequence matK phylogeny. Mol Phyl Evol 59:556–566 of Angiopteris evecta (G. Forst.) Hoffm. (Marattiaceae). Am Fern Kuroda H, Suzuki H, Kusumegi T, Hirose T, Yukawa Y, Sugiura M J 97:95–106 (2007) Translation of psbC mRNAs starts from the downstream Rothfels CJ, Li F-W, Sigel EM, Huiet L, Larsson A et al (2015) The GUG, not the upstream AUG, and requires the extended Shine– evolutionary history of ferns inferred from 25 low-copy nuclear Dalgarno sequence in tobacco chloroplasts. Plant Cell Physiol genes. Am J Bot 102:1089–1107 48:1374–1378 Rüdinger M, Volkmar U, Lenz H, Groth-Malonek M, Knoop V (2012) Labiak PH, Karol KG (2017) Plastome sequences of an ancient fern Nuclear DYW-type PPR gene families diversify with increasing lineage reveal remarkable changes in gene content and architec- RNA editing frequencies in liverwort and moss mitochondria. J ture. Am J Bot 104:1008–1018 Mol Evol 74:37–51 Langmead B, Salzberg S (2012) Fast gapped-read alignment with Bow- Ruhlman TA, Jansen RK (2014) The plastid genomes of flowering tie 2. Nat Methods 9:357–359 plants. In: Maliga P (ed) Chloroplast biotechnology: methods and Laslett D, Canback B (2004) ARAGORN, a program for the detection protocols. Methods in molecular biology, vol 1132. Springer Sci- of transfer RNA and transfer-messenger RNA genes in nucleotide ence + Business Media, New York, pp 3–38 sequences. Nucleic Acids Res 32:11–16 Rumsey FJ, Vogel JC, Russell SJ, Barrett JA, Gibby M (1999) Popula- Lehtonen S (2011) Towards resolving the complete fern tree of life. tion genetics and conservation biology of the endangered fern PLoS One 6:e24851 Trichomanes speciosum (Hymenophyllaceae) in Scotland. Biol J Logacheva MD, KrinitsinaAA, Belenikin MS, Khafizov K, Konorov Linn Soc 66:333–344 EA, Kuptsov SV, Speranskaya AS (2017) Comparative analysis of Schuettpelz E, Pryer KM (2007) Fern phylogeny inferred from inverted repeats of polypod fern (Polypodiales) plastomes reveals 400 leptosporangiate species and three plastid genes. Taxon two hypervariable regions. Plant Biol 17:255 56:1037–1050

1 3 Journal of Plant Research

Smith AR, Pryer KM, Schuettpelz E, Korall P, Schneider H, Wolf PG plants: gene content, gene order, gene function. Plant Mol Biol (2006) A classification for extant ferns. Taxon 55:705–731 76:273–297 Song M, Kuo L-Y, Huiet L, Pryer KM, Rothfels CJ, Li F-W (2018) A Wolf PG, Karol KG (2012) Plastomes of bryophytes, lycophytes and novel chloroplast gene reported for flagellate plants. Am J Bot ferns. In: Bock R, Knoop V (eds) Advances in photosynthesis and 105:117–121 respiration. Genomics of chloroplasts and mitochondria, vol 35. Sugiura M (2008) RNA editing in chloroplasts. In: Göringer HU Springer, Dordrecht, pp 89–102 (ed) RNA editing. Nucleic acids and molecular biology, vol 20. Wolf PG, Rowe CA, Sinclair RB, Hasebe M (2003) Complete nucleo- Springer, Berlin tide sequence of the chloroplast genome from a leptosporangiate Sun Y, Moore MJ, Zhang S, Soltis PS, Soltis DE, Zhao T, Meng A, Li fern, Adiantum capillus-veneris L. DNA Res 10:59–65 X, Li J, Wang H (2016) Phylogenomic and structural analyses of Wolf PG, Rowe CA, Hasebe M (2004) High levels of RNA editing in 18 complete plastomes across nearly all families of early-diverg- a vascular plant chloroplast genome: analysis of transcripts from ing eudicots, including an angiosperm-wide analysis of IR gene the fern Adiantum capillus-veneris. Gene 339:89–97 content evolution. Mol Phylogenet Evol 96:93–101 Wolf PG, Karol KG, Mandolib DF, Kuehld J, Arumuganathane K, Thorvaldsdóttir H, Robinson JT, Mesirov JP (2013) Integrative genom- Ellisa MW, Mishler BD, Kelchf DG, Olmstead RG, Boore JL ics viewer (IGV): high-performance genomics data visualization (2005) The first complete chloroplast genome sequence of a lyco- and exploration. Brief Bioinform 14:178–192 phyte, Huperzia lucidula (Lycopodiaceae). Gene 350:117–128 Tillich M, Lehwark P, Pellizzer T, Ulbricht-Jones ES, Fischer A, Bock Wolf PG, Roper JM, Duffy AM (2010) The evolution of chloroplast R, Greiner S (2017) GeSeq—versatile and accurate annotation of genome structure in ferns. Genome 53:731–738 organelle genomes. Nucleic Acids Res 45:W6–W11 Wyman SK, Jansen RK, Boore JL (2004) Automatic annotation of Tsuji S, Ueda K, Nishiyama T, Hasebe M, Yoshikawa S, Konagaya organellar genomes with DOGMA. Bioinformatics 20:3252–3255 A, Nishiuchi T, Yamaguchi K (2007) The chloroplast genome Xu J-H, Liu Q, Hua W, Wang T, Xue Q, Messing J (2015) Dynamics from a lycophyte (microphyllophyte), Selaginella uncinata, has of chloroplast genomes in green plants. Genomics 106:221–231 a unique inversion, transpositions and many gene losses. J Plant Zhong B, Fong R, Collins LJ, McLenachan PA, Penny D (2014) Two Res 120:281–290 new fern chloroplasts and decelerated evolution linked to the long Wicke S, Quandt D (2009) Universal primers for the amplification generation time in tree ferns. Genome Biol Evol 6:1166–1173 of the plastid trnK/matK region in land plants. Anales Jard Bot Zhu A, Guo W, Gupta S, Fan W, Mower JP (2016) Evolutionary Madrid 66:285–288 dynamics of the plastid inverted repeat: the effects of expan- Wicke S, Schneeweiss GM, dePamphilis CW, Müller KF, Quandt sion, contraction, and loss on substitution rates. New Phytol D (2011) The evolution of the plastid chromosome in land 209:1747–1756

1 3 Electric supplementary materials

Title: Full plastome sequence of the fern Vandenboschia speciosa (Hymenophyllales): structural singularities and evolutionary insights

Authors: Ruiz-Ruano F.J.; Navarro-Domínguez, B.; Camacho J.P.M.; Garrido-Ramos M.A.

Journal:

Journal of Plant Research

Corresponding author: Dr. Manuel A. Garrido-Ramos; Departamento de Genética, Facultad de Ciencias, Universidad de Granada. Avda. Fuentenueva s/n, 18071. Granada, Spain.

Tel: +34 958249710 Fax: +34 958244073 E-mail: [email protected]

Content:

Figs. S1–S2 Figure S1. Detail of mapping performed with Bowtie2 of the gDNA library in two regions of the V. speciosa plastome with truncated genes. Note that coverage in these regions is uniform, showing that truncated genes are not an assembly artifact.

Figure S2. Full plastome sequence of V. speciosa . The five 27-bp tandem repeats comprised in positions 44286-44420 are labeled.

Figure S1. Detail of mapping performed with Bowtie2 of the gDNA library in two regions of the V. speciosa plastome with truncated genes. Note that coverage in these regions is uniform, showing that truncated genes are not an assembly artifact. 1 tctcctgtat attcgacctt ttgaatagta tttcagtgaa aacagaacaa ggatcttata 61 tcttattctt attagcaaat tagcaaatct acccattgaa tttctctatt ccctccctgt 121 tattaatcta ctcgatcata ttgagaggaa atgagcatta tgacatagtg ttcattcggt 181 agtcctggaa aacagaaaga tctttctcga gattatctcg agatcctatt cattttcctc 241 gatactaatc attcggaatc agtatttgta gtatcaagat gtggaataaa tacagaattc 301 ttcaatactg aacccattca atgaatagta atatctattt gcttctagta aggttaaagg 361 cgaaacagaa tctcctaatc cctatcctta tccctaatta agggatacgc cgttagtcaa 421 tcgattcaga gaatagggag agaagagcaa ggaatagaaa caaagagggg aggggggttg 481 ataagaatca aatcgagtag agcatgggga ttcttttcta aatccttatg tatcgaggat 541 cgaggaacaa ctgaagagag aggaaaaagg tttgtccttc tatgctgaat agagattctt 601 catatagatc gaatggcgag gagccgtatg aggtgggaat ctcatgtacg gttctgtaga 661 gtgacattga aaccaactca tccgtcaact attccacaac tacaccaaaa aaacctaact 721 ctgccctacg taaagtcgcc agagttcaat taacttccaa gttcgaggta acagcttata 781 tcccaggtat tggccataat ttgcaagaac attctgttgt ccttgtaagg ggcggaaggg 841 ttaaagactt acctggtgtg agatatcata tcgttagagg aactttagat gctgtaggcg 901 taaaggatcg taaaaagggg cgttctagtg cgttgtagag cattgtcgta acttgtatca 961 tcgcaatgat tccgtatcaa ctcttctgat atgttaaatg gatagctgac ccgatagtag 1021 aatgagatta ttgacagggg acggaagcct gctattacag agattgatta taatccatct 1081 attcgtgtaa aacgacttga tatctaaggt attacaaatt ccattctaac atcctagtcg 1141 gttgagtctc acctggactc ctttctatcc ataagatctt tgaatgtctc ctctctctta 1201 gaacgggttg ggactctaat cctgaatctt caggaaagat tttgcatcta gtaattggat 1261 caataaacct acggatagtc ttagtaagat gaatgaaata caagattctt tctcttctac 1321 tcataagaga atggagggga aacggatact caatgcataa cgagtaaata tgattcaccg 1381 aagcaatcca gaattccttc atttctctct ctattgaatc atatggaaag ctgtattcga 1441 tgaaagtcgt acgtacagtt tggagggaga tcctcgacta accggcggat ccaccctaca 1501 atatggagtg aagaagccaa aataagatga taatatacaa ttggttggga taggattgag 1561 atctgacaca cctgcaaacc catttcacat cggagcaata tagtgaacaa aaagagaaat 1621 atagaatcgg atccaattta tcgcaattga ttagtaaata tgttggttga tcgtatcctt 1681 aaggatggca agaaatcact agcttatcaa attctctatc aggctatgaa gcgcatcaag 1741 gaaaagacaa ataggaatcc actgtctgtt ctacgacaag caatacgtag ggtaactccc 1801 gatatagtaa cagaatccaa acgtgtaggc ggatcgactt atcgggttcc cgttgaggta 1861 gtacctgcag aaggaaaagc tttggctatc cgatggttat taatcgcgtg tcgaaaacgc 1921 tcaagtcgaa gtatggctct aagatcaagt gatgaattaa tggatgctgc cagaaattat 1981 ggtagtgccg tacgcaagag agaggagact cacaagatgg cggaagctaa caaggctttt 2041 gctcatttcc gttagtctca ggtttgtctc aatccacaaa gaagagattg tggattgaaa 2101 ccttcagcct tctcttatag agcgcagggt attgagaatc agaatacgcg aggaacatat 2161 ggacaaacag aaatcagaga atgaagaaag agacgtatct aaggcataac aaggaagaat 2221 ctagaatagt aatattactt agttctaaga tatctgagaa agaaacagtc tatctatctt 2281 gtctcttttc caattcattt acctgaagat ttctctattg agatgctatg gttttcgaga 2341 tatagaacaa ttccaagaat tggttgactt aatcgactaa atacggaata cgagaataat 2401 tactctattt ctaactatca gatttatact atgaaaaata tcgatccttc tctcttctat 2461 cgtaattcat cgattcttcc agaatgtagt ttgattatca gcctaataat aatcgttatt 2521 atcgattcaa tatctaaagg aaaagataca cttctacttt accgaatatc attaacatcc 2581 ttagtcacta gcataatact cttattgggt caatgggaaa caggatctgt tccgattgcc 2641 tacggcagtc tccagatcaa caactttaac aatatcttca gactactcct cttgatctgt 2701 tcattattat ccgtcttact atcagtcgat tacatacgat gtacaaaaac agctttaaca 2761 gaattcttat tattcatatt aactgcaggc ttagggggga tgtttctatg ttgtgcaaat 2821 gatttggtaa ctattttcgt agccttagag tgtttaagcc tgtcttctta tctattatct 2881 ggatacatga agaaggatat aagatccaat gaagcgacga tgaaattctt attaatgggt 2941 ggagcgagtt cgtcgatttt agtttatggt ttctctttat tatacggatt atccggtgga 3001 gagactcaac tctataagat agtcgatgga cttctatcta ctgaaatgta taattctatt 3061 ggaatctata tttctatcac gtttattgtg gtagggatgg gttttaagct ctcattagtt 3121 ccatttcatc aatggactcc tgatgtatat gaaggagtgt gattcgttcg aagaagaaaa 3181 tcgactcatt ctagattctc ttggaagatt tgattgactt ctgtatgaca tcctacagac 3241 atgtgaagag gataggaacc tctccaaatc accaattgga tgaatgacta actcttacca 3301 aaaaccccag aaatctgcga gaaacactta acacttgaat tgtttcacat aagtaaggta 3361 gaacagagtg aagagagaag tacaacccaa tagaatagag aactcttctc tatctcttga 3421 tctttctcaa agttccattt attaggggta ggtgtcacga agaatgaata gatgatgcaa 3481 cggaaagaat atcttctcta ttatctatag aagaggattc ttttctttct gaaaatgtca 3541 atccaaccag tttccatcct tcagggactg taggtatagt aaaagaaagc attcgtcaaa 3601 aaagatcgcc ccaagataat actatcatgc ctgttaagaa cgaagaaaat catattcttt 3661 ttctcctatc taagatctct cttagattcc cctatctttt cttggttttg gaatagagaa 3721 aaggagaggt tagagaatgg atggagactc tagattaatg agaaaggatt cctcgagaag 3781 ctaacaacca attagtactc atcttgaatg attaagaaag aggatctaga agaagtagat 3841 ctgaaacata cacgaggaag gttctaagag caataagagg aagaaatctc ttacgaacta 3901 ggagccgtgt gaagtaagaa tttcatgcac ggttctgaat gagcggtaag atcgaggatc 3961 ttctttccga ctctgactct cctacaccag ttgttgcttt tttttctgtt acttctaaag 4021 tagcagctct cgctttattt acacggatct tccgtatcat tttctcatat ctatctggtg 4081 aatggcatat tgctttagga atattagcta ttcttagcat gatattggga aatttaatcg 4141 ctgttactca aataagcatg aaacgtatgc tagcataccc ctctataagc caaattggat 4201 atattatgat tggagtactt gctgcagatc ctgataatgg ctatgcaagt atgataacct 4261 atacgtttct ttatatattc atgaatctcg gaacttttgc ttgtatcact ttattcagtt 4321 tacgaactgg aactgataat attagggact atgcaggatt atacacaaag gatcctgttc 4381 taacattctc tctagttcta tgtttactat ccctgggggg tatccctcca ttagcaggtt 4441 tcttcgggaa gctctatctt ttttggcatg gatggaaagc tggtttgtat tcattagttc 4501 taatagcgct cattacaagt gtcatttcta tatattatta tccaaagata atcaagttaa 4561 tgttcactgg aaagagtgac acatcaacaa tttacgtaca ggatccatca gtctcttcat 4621 ctacttcaat ctcgaagagt tcaattgaaa taactatgat tgtttgtgta ctagcatcaa 4681 ccttattagg tatcttaata gatcccatta ttgagattac acggaatacc ctattctaga 4741 cccatttcaa tttgtcccat gaaagaatgt ggaataatgg aatctttctg ccagtgcaag 4801 aaacttctgt gaaagatact gccgatatat ccttcttgtt tttgctatcg aatagccttt 4861 ctccttatgt tctagaaaga aagtgaaatc ttgaagaatc atagaacgat catctcactg 4921 tccttatcat tcgaatagat tgggaatgga aagacaaggg ccctccccaa tctctccacg 4981 ttgttctgag tttggccacc ctggcctggt attgtctcga tcagagagag ggtttataag 5041 catttgatac tccttgcaat cgaaatggaa aggttttagt agtcttcaaa aaactattca 5101 agattcgttg aaccttgaga aatctttcgt tttcctaaga ttccttggaa aaaggaagaa 5161 ctctatttgc tcatcaatag agctcgagat ctttgcgctt cggtatccag gaagaattcc 5221 attctcacgg tctgtcatcc tactcctgtt gattggagcg gtggaaggaa tgaatcaaac 5281 gaataatcga ggaatgggta agagggatct cttcgattca aagaattcgt ccattgatcg 5341 taataggatt tcttcggtta actcggagat tgacctcatc tactggcatt gaaatctact 5401 ggcattgaat cgttcactcc cttctctttg tttttgctcc aatcctatga tattgagaat 5461 attaagcgga gtctctctca atgccccccc ccttctattc ctcgttccag tagctatcct 5521 tggattggct attggaactg tattcattgt ccgtattggt ttcttaatcc ttttcttgac 5581 cgaccaagac tctcttcttg tgctggtctc tcaagtcttt gggaatctga tagcgtcttt 5641 tcccaccatt ctcttcttcc aaataaagat tctggaagga actacacccg cagggtatga 5701 ataggatgat tctaagaagt cctggtagga aatagtgata ggtacaagga attgagagat 5761 tatcccagtt gacagggtag aattcaatct tygcgtggca ctctcttcct ccaccaactc 5821 tctttgagac caacatttct ctactcttct cttccttact ctgagcgatg ccgaaaatac 5881 ggcattatcc agatgcaaga tctagaatag aaacgtaaac ggagtctgag ataactttgg 5941 aatcacaatt attggggact cgatactgat gcaataccga cactagaaaa acctctgcca 6001 gaatataata ttgggatcta cgcttccaaa gtagaatatg attcttcttg ttagaagaat 6061 aatgatgact cgtctagtct attccggatg agatagaagt atttcccaag agtatctatg 6121 atattggata ttgtccaatc ctctgcagta ttccaagcat cgaattctct cctaatggaa 6181 ggggttagat acgattaagt gattttcaac gaatcattga ttctatcatt catggatctg 6241 gtaaactcta gattataaca cgaataatat gcaggttcga tatgttcgtt ggtgcgcatg 6301 gtagtcaact ccaattgtag ctctcatttt tagagacaat tgcttaataa tcatctgatc 6361 tagctcattc ggttgatcca ttagttagct atcatacata catgagactg gattttcatc 6421 ttcaacattc tatctctaac ctcttctaga agaagatccc aaagtactct ttattcagag 6481 gtttctcagt caagaaatag tcttcttaga tagtcagata gagaagtctc tgatttgatt 6541 ctttcacggc atggaaaggt tggatcgatc tggttcgatt tgcacctttg aatagattct 6601 gctacctttt caaggacaat taggttgctc cttttggagt attgtgatag actatagtca 6661 ctcaatttgc ttctccctag gtctgagact gaaaggatct atatcaacaa agcttgacaa 6721 gtggagagtg atgggatata atactatcca agaagcaaag aaacaccagg gtttcttctc 6781 ctcctaatca agctacaatc aagaagaaat acgtcttaga tgctgaatca atagggaagg 6841 atgaaaggag aggaaagagg gaagcctcga tagtgaaacg gtagacacgc tagattcaga 6901 atcgggtgct aaaaggagat agaagaagca tggaggttca aatcctcttc gaggcaacag 6961 gtcttgcatt ccacctccag gagtcggaag actcctcgtt cctcaccaat ggaactagga 7021 attctttggg ttggtgcttt caaactctca atcctatgat cagacgggac ggtagaagga 7081 taggacctta ggattggatc caattagatc ctgagtgaat gattgagtcg gatcctaagc 7141 taagagtaag agatccaacc tagtgtggtg gatgaatttt tagaatgtaa agataagaat 7201 agaatactct ccttcccatc tctatcgatt actgagagat ttacaaattg actcagtaaa 7261 agaagaacag aaactaatat attccttcta ctgaatcaat atgtaaatca atctctagag 7321 tgtagattga ttcgattgaa tatggaatta caaacaataa tctctaatat ccaattgatc 7381 ggttacacaa caaaatggca ttctatcaga tccgcataaa tgaattagat actgataact 7441 ctcaagtaac atattgtaag tgagaaaata tctgcaagag aattgatttg atttcaacca 7501 tctttctcga taaatcagaa tactagattc aatgaatcga tcttggaaat cttctattaa 7561 gatagattga tacaagtgaa taggaacaaa cagttgtgga tactgcaaat ttatatatat 7621 agattgaact ttactaatat attcaaaaga ttgttcctca tccaaaggat aattatccca 7681 tcgattagta gataactgag ttatggatct acgatcatat ccgcgataaa gaaagaaaga 7741 ttttattttt tcatttgaaa aatgcttctc gcgctctctt cttataccat aagttatata 7801 gagtcttcgt actaattcct gcttcactaa taaattacga tatgaagaaa caatattata 7861 atctagatag attgggagta caggatccca aacaaattgt tttcctaaga atacaattgg 7921 ttcaggagat ggatttaatt gagttttgta ttggttacat atttcagaaa ttcctaatcc 7981 agcgaatctt tcacgtaata atatattatc caattgattt tctaatttct taatctctct 8041 ttttcttaag tttcctaaag atcttctgta accaaaagaa tatttttttc ccttatataa 8101 tttctctttt ttatacttaa tcttgttcag atcgaaatcg cgttgaggaa tttgaccctg 8161 atcttgataa aaaaggaaca tattgtctaa attattggat tccgataata atcttatgga 8221 ttcaaaacta caactccgcg taaaactcag atgccacatc ttaggagacc aagcaatctc 8281 acgtattgtc cccgtcagta ttgaatccat tctaactagt tgtttagaat tatgaatatt 8341 agacaaatgt ccttttacgg gtttagagga ataagcctga taaattatgc ttgaagaata 8401 gctgcgatca ataagagaac caaaggggag actattcttt tgacttgtac ttcgattaat 8461 ttctgaacag aagaaatctt ttgaaaggat ttctagaata ccacaagcta agttaaaatc 8521 attctcttca gctttattaa ttccaaaacg atcctctttc tttctaacgt gcaggttgaa 8581 ccaacaatcg cgggccgcta atccaactag taaaaacaag atatacggaa gtattgttaa 8641 ttcgttgatc gtcgattcag ttctagaagg ttctaaatgc caattatata ggtaatagaa 8701 tctctttttg aataaattag tattcacaaa ggaaagattt gtaaaaggaa tctttaagaa 8761 actattcttt agaaaagatt ttgctatctt ataagaaagt ttttcaaatt ccgaactaga 8821 ttggtgttta ttacctatgg tattaacaat tgaattctgc cgaagcaagg ccagttcgat 8881 cgcatcactc catagaatcc tttctctttt tctggaatta gttattaata atgtcccatt 8941 agcaaaataa gaaaaatctt gtttactata acccatggtt attgaatcaa ttccttggag 9001 aagaagtgga ctaatctctg tttcaaaacc tttgatacgc aatagcatcg acaattcttg 9061 gcggcgttga cgcccagtcg atcttcggaa atttatcagt tgattcaacc gattaggggc 9121 tattagagct ggatccagct ttcgaggtag atgagttaag gcaatgataa gattattaat 9181 actgcaagac tttctatgat cattactaat cttttttaat attatacaaa gcaagaatct 9241 gggattagct tctaatctat gatagaaacg atcaatattc aattcatgaa tatcttgaat 9301 ccatattata caaggggata gctcttttgc tattgcaaag atgagattta accgatgtac 9361 actctgtctc gaaataaatc ttccccgtat attaatgaga aacgatctat tatacaacaa 9421 cttctttagt ggtattctta tcaatggaaa agaagaatta gacgtgatat ttctaataat 9481 aagagctcgc ccagtctcta taggtcctac taatagaatt ccttgaattg gtaataatcc 9541 tgattgaaat gaaataggca tatcacgata tggaatattg ggaactcctt ttcgtttcat 9601 tattgctgag aataagatat tcttcccaag ggcagaaaga acccactttt cggcaagatc 9661 cgatttacgt attttgtagc tcaatagatc tttttgacaa aatcgaggta aatacgatag 9721 aagatggaat ccttctagca ggaaagagct atcaacaaga ttatctacca aaaagttaga 9781 atcagaaatc tgcgacccgt ccacatattt gcaaatagtc tgattagtta caaggtattg 9841 agttaataat tgccttttaa ctctaagagt atcgggactt cctttttgaa gtatccaaag 9901 atccaatctg cttctcacaa agaggtagaa gaacaaatta ttcatataat tcaagaatct 9961 cttaaaagaa tgtatgaata gatatcttgt ttgcatttgc tttgtcgaag gggaatacat 10021 tacttttttg agataaaagc tacgcattgg atctattaag tatctaatag tatgaaaatg 10081 attccataag taaaaaggat tcaaacccaa aaccggagac aaaaaatgct taaaaaatag 10141 aagaacaact actattaaaa gaagagaata agataggatg ggcttattta aacatctcaa 10201 tattgaccat cgggaacatg ttgtcttccc ttgatttgta agttctttaa agataataaa 10261 atcaagccta gcccatatct tcttaataga atcccttttt aatatcagtc tgaaatctgc 10321 taaaaaatag actagagtat ttcttgtatt gacagaaacc ttgttgggaa agttcaattt 10381 ggattgatca ctcagattga gcattatttc tggatatgtc tccaccatca gatcatagaa 10441 atatctccac cagttggaag taaaaaacca aggtatatac tttctaaaaa gaccccattt 10501 ctgacaaata tcatctctcc agaagatcca acgaacccta aagctgctta aatattttga 10561 taatctattg aaattgacga tatagcgtgt atgcctggct tcgtctcgga tatacgtatt 10621 tatatctgta aatattgcat ctttatataa agacttagtt tttgttagaa acattgatgc 10681 tatattttta tgtaatgata accttttcaa ccaataaaaa aactcagggc ccctcgactc 10741 aaaaagagac acgatataga aatcaaaaga atttcgagag gtttgatcct caatcaaaat 10801 gttcctcgaa ttcaatctct cattcataga tttctctaaa ccgaaggaac cttttctatt 10861 ttgtaaataa agatgactct gtgacctagc tagtatctta tatcgctctt ccaagaaaga 10921 ttttgttgtt ttctgattct tctctattat tttgtataat cctactaata aaccagaaac 10981 ttggaactga atatctgata gtacttcaga ttcatccagg tttcttggta gaaagaatcg 11041 catgactcca tcattatgga agcttatcca caaagaatct aatatattgg aataattctt 11101 actacaattt tcacgaaaaa aattccttct aattcgtgga atattagata tttgagaatg 11161 acctgagaag tgactctcat tacccttctt taactcgatt tcagaatctg aataatctag 11221 aaacggagga tcccaatcct gtaacatttc agactcgatt aggtgactat cctttctttg 11281 aaggaccttt ttgaaagaat atctattttc gtcaatattg agtaactcta caaagaaatc 11341 aggcaacaca agatttctat tattcagttt agagaaagaa gaatcaatta tatccaccag 11401 aacatcttta gatataatct taggaagaat aattcgccaa ataaataagt gattagccaa 11461 tttaacagaa taatcgctag taatattaat atcactgata caaataccaa aatgatccat 11521 taatctgtct cttattcttg atatatcata aatcgatcta tcagaataag atcttaatct 11581 aaaaggtact ctcttaagag aaaaaagaga tgaatcttta attatattaa agaatctgtc 11641 catagttaat tgaacagttg tatattcatt aatatcatta atctgaataa taatgaatct 11701 atttaataac tctttccagt ccaatagatc ttcttgagtt acgagcttca gtatattagt 11761 atattgattg ccttctgccc agctaacgga tagatatctt aattggagta tgtacctaat 11821 agctactgta aagaaataat catataaaaa aagaatatag atcaaacaga aagatattat 11881 tctatcttgt tcatataact caacaagcga atagattgaa tatgtaatat atctttgctt 11941 gaattgggcc cagaagatag aactattgct tctcctggtt atttctttga gtatatgtct 12001 atttctaact atttcagaat aattagaaag gatcttctta gaatcgatat atttgttact 12061 tcgtatcgtt aaattcttgg aacatatcgt attcaataga ttgattctga atataacagc 12121 tctcggagat cccctagtat aatcatcgat actaggaata agattcaatc tattaaaaac 12181 taagatcaat ctagttaatt gattgattga ttcatgcttt atttttgaat aaatatatgg 12241 aataaaaact ctcttaagct ctccataaga atctaatctt aacaattcag agaacatatt 12301 agaattatta cagtcttgaa ggatatattg ttgattctta gaattatcct tgattacttc 12361 tgttaaacaa agaagtgaag ggtgatccaa caatgaaaga ggattgaatg taagatcaga 12421 atattgagaa gatctcttta tctcccatag agtgaataga tctttgatac ttaagaatgc 12481 agaatctttg tatcgttctt ctatcaaacg tgtattttgt aaatccaagt tatctgtcaa 12541 agtattctga gatcttctga taagacccgt cttacttgag taatacatag acattggtag 12601 tgtcaatgcg agtataattc ctagaacaag tctattatct cgttgtatca aattacgtag 12661 atctcttaag aggagcggat tcaatattca tggatcaaat agtttaataa aaggttcaaa 12721 gccatataat agatcgatca aggatctttt ttgatttcga ttctcgaatg atgtaaagaa 12781 ggaatggaat ctttttattt ttgttaatct taaaactcta tagagataga gagaagacgg 12841 atctcttact ctttttcctc ccacctctcg caaattactt tctttcttca tactatatta 12901 ttgtgataga tactatctgt ttcttagatt ctattatata gataatgtta aaagattcaa 12961 ggttcaattg attattgctt tgattgaaat gattcactga tatgttttta atctacaaat 13021 tcaatcttgt tagattgtaa gatcgaaata gttgaataga tcaattattc tattcttaat 13081 gtttctatca gtatattcta agctatgact tccaagaatt agaacaagtt agctaaatag 13141 attcaatggg cgaacgacgg ggattgaacc cgcgcataat ggatccacaa tccattgcct 13201 taatccactt ggctacgtcc gccctctctt aaacaaccca aatggacaag ataacagaga 13261 attatttcat tgataaagat aatacatatt atcatcagat atctatctga ttgtggatag 13321 atccgcgata ttgtattcta gataaccaaa tagaaatcgg attggataac ccttgtaaat 13381 gtttttcaat aattggtaaa cattatgtta tgcttcaata taatatagaa aaaatattat 13441 tggatcctca tcagtgacca gtaatgcaat tattacatct ttatttatct atctaaagac 13501 ttctctttgt atccttgaga ttccatcatt gaagacatag aattgtatac tcgtaataga 13561 gtatatatga agaatgctcg tattattccc atttatctac tagaatgaaa tccgcaccaa 13621 gtgccaaccc ttgaagggtt ggcacttgat tacactgcag aaccaaatag atattatccg 13681 tttacagaag gagcttcaac agaagctaga tctagaggga agttatgagc attacgttca 13741 tgcataactt ccatacctag gttagcacgg ttgatgatat cagcccaagt attaataaca 13801 cgaccttgac tatcaaccac agattggtta aagttaaaac cattcaagtt gaatgccatg 13861 gtgctaatac ctaaagcggt aaaccagata cctactacag gccaagcagc taagaagaaa 13921 tgtagagaac gagaattatt aaagctagca tattggaaga tcaaacggcc aaaataaccg 13981 tgagcagcca caatattgta ggtttcttct tcttgaccaa acttgtaacc tgcattagca 14041 gactcattct cagtagtttc tctaatcaaa ctagaagtta ccaaagaacc atgcatagca 14101 ctgaatagag agccgccgaa tacgccagct acacccaaca tgtggaaggg atgcataagg 14161 atattatgct cagcctgaaa aacaatcatg aaattgaaag taccagagat tcccagaggc 14221 atgccgtcag agaaacttcc ttgaccaagg ggatagatta ggaatacagc tgcagcagct 14281 gcaacgggag ctgagtatgc aacagcaatc caaggacgca tacctaaacg gaagctaagt 14341 tcccactcac gacccatata gcaagctaca ccaagtagga agtgaagaac aatcatttcg 14401 taaggaccac cattgtatag ccattcatca acagaagctg cttcccagat gggatagaag 14461 tgtaagccaa tagctgcaga agtaggaatg atagcaccag aaataatgtt atttccgtac 14521 aataaagaac cagaaactgg ttcacgaata ccatcaatat ctacaggagg agctgcaatg 14581 aaagcaatga tgaatacaga ggttgcggtt aggagggtag gaatcattat aacaccaaac 14641 cagccgatat aaaggcggtt ttcggtgcta gtgatccagt cgcagaagcg accccatagg 14701 cttgcgcttt cgcgtctttc taaagttgcg gtcatggtga tttttggttt tcgagataat 14761 tattgagtcc ccaagccatt gagcttgttg atcggtagta tagcatggaa atcaatttgt 14821 gtcaactcat gtttattcca cattattaag cataggaggt atttttcaat gctaataata 14881 tctttattct ttcttttatc aagcatattc tttgcttgga ttgaactaga atcgaacaca 14941 aagaaagaac tttaataatc ctcttgttat acttctaata aaaacagaat aaaagaaaag 15001 tcattcatta ttagaatata tcaactagag aaggatatag aatagcaatt attctgtaat 15061 ataagcaaat agcaatgatc tattactccc aatcttagtg caatatcgtg gtaattatac 15121 tttcctccta atagataata atcatatttg atgcaataca aatagttgtt gcttgggtaa 15181 ggatattaat aaaacaggaa gagataagaa ttgattgtta tcttttgatt acaataaaca 15241 aataaataac aattcaacct agaacttgtc agccttatct ggatggcatc ccatgtcgct 15301 tatgattcaa aagtaagaaa aacttatcaa ctcttttctt taaagtacat agaattcttt 15361 atctttttct atcgatatct attctattcc aattatttgg agaaataatc tacaacctac 15421 tttgatattc tatatactat ccaataatga gaactgagtt atctctaaat accagaatct 15481 ttgattcttc aagatattct ttgtctgtaa gaaagcagat aatttgtagt ctcttaaaga 15541 agagaaagtg tttaaaaata ttctcaaatc aaaccttcgt cgaatcgtgc gtattgtacc 15601 tttatgttta caagctaagg ttttagcaca agaaaatcgt agtatgtacc ttaacctata 15661 caagctatct ttatttattg atccactata atagaaatat ataatcttcc aaatctgaac 15721 aaatcttctt aagatctcat tatctgttaa cgtagtccag gctgatctac taacgagatg 15781 tccggaattg tcgcaaaaat tctctcttac catatatccg attagaaaca taattgggac 15841 attaaagaat gattcattat taatagaaac agtaatatat gaatccttca tagttccaac 15901 ttgaacttcg tttatttttg atcgaacgcc taggatataa cctaagaata agacacaatt 15961 ttttgaaagt cttttggcac atattcgata gcaatggagc caacgatgga aattagattc 16021 ggcggatttt aggacgtaat agatccacct tttcactgaa ttcttagtcc ccttaagaat 16081 gacaagagaa cgattttcgt atcttccata atgaatgcag aaacctctag taccctgagt 16141 attctctctt aattcaatca agaaagattt accaatttgg ttctgttttt gaaggagatt 16201 ataataatca gatatatgaa tgagatattt ccatcgtaga ccagaaaatt gtttccataa 16261 aggcactact aatgattcaa tctcgtacat gtaggaattc cataccaaaa tggctaaact 16321 actctctgga cggagataat ctattgaaaa cccagggccg gtatacatat gaaagatcaa 16381 tcttggtaga tgtagaaacg gagcatcctt gattcgatgg cgaaacattc gaatcgaaac 16441 ctctggatgg agggtatgag gtatctttgt ttctaaaaca taattagaat gccgaaatct 16501 atcttccaag aatggaaata tcgaatgatt ggatcgcgaa cttttccatt cattgatttc 16561 tctcatcaat aaaggttcta tgcaaggtga tagaacgttt tctaagaccg agcatattcc 16621 ttttgaaagt gcatcaaaat acatatatga accaccaaat caaattgaat catattctgg 16681 agagaaaatc cccgaaagat cttgttgccg gattgccttg atgagacgtt ttatagatat 16741 tatactatat ccattagaaa gattagagtc ttttctcaaa tccatactta gtctatttga 16801 agaacgatta gaaacgattg agtaaaggtc atcttggaaa aggagagaat acaagaaata 16861 ctcccgcgtt gtatttatca cttttctctt gcgtaacttg ccaatcttcg acaagaatcg 16921 atatccagtt ctcatacaaa taacctcttg attataaaga tttcacatcg tggatctagt 16981 tgaaggattc attggattaa taattcttcc ctaggtcttg tgaatccata tttgtctatt 17041 cagtcaattt gttttattct aaaataaggg tgaatcactc ttgttactaa tttgaagatc 17101 aagcattaag tgtgctctca attcgcatgg gttgagagca cagattattt gatttttcta 17161 cttgggtaat tctcagtgat tcctataact acccaaatct ttagaaaata gttgtgataa 17221 atatggacat atcttatatc taaatatgtt atctcttctt tactactact cgaataagct 17281 tgctattctc tctaattaat tggttgaaaa gaaaagactt ttgagtgtta aataatcgat 17341 gaatagacct tcaaataaca ctgtatgctc tcttctttcc tttgattcca tcttcttttc 17401 tgatgagatc tttattcttt ttatatctta gtaatacctt aactctaact gagtcacaat 17461 tattatttaa ttctatttca ttcaataact ccattagaaa taagactcta gaatgcagga 17521 tatcgattga tctttaagaa cagaaagaat gttctgtata tatttacaaa tatcaactga 17581 attcaattgg aaagatagat ttggattgag atccgttttg atccaatctt ctcaagtaga 17641 cggtaaaatc aacgagaaga tatcttcata acttatgact gaaaagagca ttaacggcgt 17701 cttgtctatc tatcctttgt gaattgtaaa taatttctcc atctattgct atttattgat 17761 tgatctatgc tattaacgtt ctctatttat tgtttttatc cattaaagaa tctatgaatc 17821 tgctaaaaaa atccttcttt acaaaagatt tgatagagaa ccaatacttc tttatggaaa 17881 gacgacttct tagaggaatc acaaatacta agtagatata gaaaaagaag aagaaaaagg 17941 gatagtagaa taacatttgt gaaataatat aaaccgagcc cattggattc tcctaataat 18001 tcacttcgac ctgttcaagc acgtttgctc gtcttattat atgatgaaca gtctgagttg 18061 tttgagctcc ctttttaagg aaataaatga tagcagaaac atccaattga atctgcccgt 18121 ttcgtggatt ataacatcca acttctttta tagcttttcc ttgccttcgc gattgaacat 18181 caatagcaat tattcgataa gtagtttatt aggataggta cagagaagag gtctttcccc 18241 ccaatgaaac cgtacatgag attctgactt catacggctt gagctatgac tctagtactc 18301 atgaaataaa caaaatatta gtaactatct attcttcgta ttccatctct tcttgattcg 18361 agagaaaatg ctcttaaccc atgtctgttt ccggaaatct atggattacc aattacctct 18421 cgagttatct ttaactaatc actattgtgg taaaacctta gaaatttgga aaatctatca 18481 ttgattgatt caactttgta tcttagtatt atctcgttcg tggatcaatt ctattaatcc 18541 ttaggttgaa gttttactcc gagggctttt gggatcaaga gttggtagca acaaaactta 18601 ttcagatcaa gccataaaaa aaaattacat ccaatagatg atccattatt tctgatagat 18661 caaagaagaa gatttcggac tctagtgtaa cctaaagatg gaatatactt atgtgaatga 18721 tacttattca atcaattccc aaaatattga atttagttct tagataagga catttttctt 18781 tgtaatcgtg gaccaacgaa gttcaataat cttcttatat tctgtagatt aaccatagat 18841 attattcctg atcggagaga tatctgggac gtttgagttc cacaaaaaca gatactattc 18901 aagtttcatt ggataacgaa tgttatcaaa tgttgaaata ggagcatttc cttcagattt 18961 gaaaatggcg gttaatagat tccgcaagaa ccatcccgat tctcgagcca cacgttgctt 19021 tccaccatat cgttttaaac gaggttttac cataatatct gaaaaaagat tatttcctta 19081 ttgcatattt gttattacaa gaatgattct ggtgaaagag accaatatca tacgaaatcc 19141 aaaagaaaag agagatatat gttcatgatc ctaattggat agattccaat cctttggaaa 19201 aagattaatc actcaatttc tctttagctg catacattac atcaactgta atctttgtaa 19261 tattattctg tcttgcaaag atttccgtat ttctcttgat tctccctctt acaaagccag 19321 gaatcttatt cagttctaat tgactctcag aagtccagtc caagtctgta tctgtagaca 19381 acaatttggt aatgacttct ttagtatcgt gaccaccaaa tacatctaat aggtgatctt 19441 ccatacccag agtgaatgag ttataaacta gatcagctat ttgattagtt ccttcataac 19501 ccaagaaggg tcgatatccc aaagggaaat tctgaatatg aacaggtgaa gaaatcactc 19561 cacatggaat atcaagccgt ttgccaatat ggcgctccat ctgggttcca aatatggcaa 19621 aaggctcgat acaagctatc gtatctccaa cttcggtatg atcttctgta attagtatct 19681 cattgcataa acttccgact tgttcactga accatttcat gtcatgttta caataagtac 19741 ctgaacaact aacatggatt cccatctctt tgacaagtat tttggtaatc gaagccgcgt 19801 gagttgcatc accaaatact actgcttctt taccagccaa attctgacaa tcgatagatc 19861 ttgagaacca agctgcttga gaaacaaatt ttgtttgatt ctcaatgtat ggtttataat 19921 gcacgctgcc ttttgatagt agaaaattcc aagcattgat atgttcttca atctgtccaa 19981 taaaatcagc tgtatccaga attcccatag gagtggttga tatataaggc attccaaatt 20041 cttcttctaa aagtattgct gtcattaaac caatttcacg ataaggaatg atattgaacc 20101 aagctcttgg taaatctttt aaatccttta aagatcctcc ttcgggaact acttgattca 20161 ctacgattcc caaatcttgc aataaacgtt ttaattcacg acaatcatgt tgattatgga 20221 agcctaaagt agaggttcca ataatatttg ctgagggttt gtctgttata gagcaatcta 20281 atctgttttg cttacgagct ttatccaaac aatgtcgaac tatttgttct aaagttcggt 20341 ctgctgcttg aagctcattc actcgatagt gattcacatc tgcaagaatt acatcagata 20401 cagaatgaat cgaagctcga tctacaaaat tttgtaaatc ttcttgtaag atgctagacg 20461 tacatgtagg tgtaagaaca atcagatcgg gacgttattc ctcatctttt cgatttatat 20521 tattaataac cttttcttga gatccacgtg ctaatacatg acggtctact atactagcag 20581 ttactggagt gaaatctctt tctctttcca acatagagcg cattacatta aaatagtcat 20641 ctcccaaggg tgcatgcatt atagcgtgta cattccggaa agaactggct attcgtagag 20701 taccaatatg agcaggtcca gcatacatcc aataagctaa tttcataaat cagtttccat 20761 tatctttttc ttccgcgtca aagggaaatc tattgctata tttaacttat catcataata 20821 taatatgacc tagaagatcc atccagaatt gaaaaagaaa agcgtcagaa tagccttact 20881 tgtcgtattc ctacctcttt ctatcatagg taagcagcat ctttttatac aacttataca 20941 tacaggatct gatctgggac ggaaggattc gaacctccga gtaacgggac caaaacccgc 21001 tgccttacca cttggccacg ccccatcaat gagcgatata atgaatatgc catagtttat 21061 cccttctgtc aatctagtta ttattttccg aatcgatcgg ttatatgaat tctgaaagaa 21121 atcaaaagag aatagttttt tgtggaaaag tctcatccat gaacaaataa actatttact 21181 tcgatagaat atcgtaaata ttgattctat ctattagaat aaagaaacct gtaatattct 21241 atacttagaa aaatgtactc aacggttcaa cctattaata attctatttc gtattaaaag 21301 agaatttttg tcattggaga atagaaatga tagttctgct taacacctat ctggtagatt 21361 cttttcaatc aaataatgct tttcttgcta agctgccaga agcttatgct atttttgatc 21421 caattgtgga tgtaatgccc gtcataccag tattcttcct ccttttagcc tttgtatggc 21481 aggctgccgt gagtttccga tagtaatcca ttcctttgtt ctgggtgggg ttaatattcc 21541 tctatacccc ccccaagaga agaagaaatc atggaatcta agaatgttaa tgatgagcgc 21601 aaaagatcgt aaagatcctc tttcaaagcc tgaagtcaag atgaatccta attggactct 21661 tttgtgataa atctatgaga tatctatgca tcgtagcatg gaagaaggga gagaaactct 21721 cgacgcactc attgaaatca atgaaattat accttctagc tcttaattca ttattaggaa 21781 taattaacca ttgatgagat tacggatatt taaggaagat agaaaagaaa aaactaataa 21841 ttacaaaaga ttggtaaaag accaaaagac acatgagaat ttacgggctt gtctttctat 21901 atatgtacca ttggataact ttatgaatct atgatttata tctcttgtga cacttttgca 21961 agggattgtt attaacaggg tctcaaaggt atagaaattt aaacttgatc aaccaattct 22021 cggttcgcta tagtcacaag agctctcaga ttcatagaat cactgattca acatagttaa 22081 tactaagatc tattctataa aaagagagat ttctattcgc cagaagagag atgatagaat 22141 aagagtatac agatatctag caaatctttg tattcctttt ctttccctct tctcgaatag 22201 aatgaaatag gtgtggtata aatactccaa acatgttcca ttctattcta cctctagtta 22261 tgatcacgga gattaggtca tgcttactct taagctattt gtctatacag tagttatctt 22321 ttttgtttct ctttttgttt ttggattctt atcaaacgat cctgggcgta atccgggacg 22381 gaaagaatag gcttattgta atagcttagt attttcgctt atcttgttta ctcaattaag 22441 aaataaaatc agtattagga gagagaggga ttcgaaccct cggtacaaag cattgtacaa 22501 cggattagca atccgacgct ttcaacctct cggccacctc tccaaacagg aaaatcattt 22561 ccactatata atcaagctaa aaaaaataag gatctatcct gtaactaaga tatcataggt 22621 atgctgcttt agaaatccat gaaagatgaa tgaaaggttt ggttcactca actttatcct 22681 taatcgattg aatcttcgga tttctagaaa tcaatcgctt tcttctttct tttcaagatc 22741 cttcagccta gccggaagat agccaggctg cttttctatt caataagaac tgattattga 22801 tacagtgcag aacccaatct tattgctaag agacttgtta caaaagcacc aataagggca 22861 agaataattt gaccgtccga aattgatgtc attagcgcat tatctccctg attatgtttt 22921 aacagatgaa gaacaataga cgatactctt ctttctcatt tgcataaatc attacactac 22981 tgtttattgt actaattcca gttcgtcatg gctatagaag atcaactctt atttaaaaag 23041 aatggttaaa aagacgagaa aaagggattc taaaccagaa aagaaaccat tctaaccctg 23101 agagggagat ttttgaagat aatcaagagg tcaataatga atttagaaat cattgcacaa 23161 cttattgttc tagcattaat agttgcatca ggaccgctag taattgctct atcagcagct 23221 cgaaagggaa atctgtaatt ttttaatacc gtctccggaa aaaggctact tttctatcaa 23281 acaccgaatt ccggagatga acatctagag aaagaaagta gaaagaaagt cttttttttc 23341 tcttagtaat ttctattcta tcgacttttt taaaatagac gcttagatat ttttaagtta 23401 taatttttat atagcgggta tggtttagtg gtaaaagtgt gattcgtttc atccttagac 23461 tgagtggtta agggatcttt caaattgatt ccttactcaa tcaagaatct ttatttctcc 23521 aggagttcac aaacttccct ataagagatg tttagtgtag ggaactacat tcctgttatt 23581 ctgaaagatc gcatttcata agtctcggaa taacagagcg gaatcctttt gagactcaag 23641 agtcccaaaa taattagtaa ctaatctatg gatagtaatc taaaatatct actttatcgt 23701 cactaaatac cggatcgaat aagaaatccc aacccaagag ttatgaaaag attaaacctg 23761 gtttaccagg agtatcaaac attgatttct tatttatttc gtcgtttaac ttggtaaagg 23821 tatattctcc taaggataca gatcaataga aataaagaac gaattaactg gaagaaatat 23881 tgatttacct attcaaccct cagattgatc gaatctaatt gattgaatcg attgaatcga 23941 ggaagagata aaacaagaat cttcaaaata ctatttcttt tatacaagga tcatctaaga 24001 agcatatctt tgttatctaa gatacaagga aaaagagcct acatagatgt tatggatgct 24061 tctagtttca atagcataat tctttgttag gaagtaacaa gatagttaag atggaataga 24121 atcagattct tatcgatctg gacgaaatag gcttcgagta ctctttattt tgtcttggtt 24181 tgattcctta tttgattcaa caagggataa gatacaaaga tgtttctatt tctaactatt 24241 cctctcttcc atctgtttct agatttcttt ccttctaagt ccatcgagga gccgaatgaa 24301 gagagatctt cacgttcggt tctgaattag aggtgttaaa cctattgatt aacatcgact 24361 ataaccctta gccttccaag ctactgatgc gggttcgatt cccgctaccc gctaatgata 24421 tttattgatc tattctacaa ctctaatctt tgtttatagc taacaaatcc gaattagtag 24481 ctgtttttgg caattctcaa tatagcttat atctcggata acttcgtatc cgataaagcg 24541 tccatcgtct aacggatagg acaaaggtct tctaaacctt ttgtataggt tcgaatccta 24601 ttggacgtaa ttctatatct gatgaggcat atgatccatt attgattaga aataattgta 24661 attgcttatc gattcttctt ttcttagtag ataaaagaag tagaatctag caacagaatt 24721 attagataag tcacttttct tgaagtaaga atagttcggt atgttcttta atagcttctt 24781 tcaaaagagt ttctgcctgt tctgtaaaca tcttagtaga acgaataatc tcaccgaatt 24841 gaggtttatt cgttgttaca tattcacgca attggactaa gaatctttta acctgttcaa 24901 cgtctaatat gtctaaaaat ccattaactc cggtataaat agtagctact tgttcttcga 24961 ccgataatgg ggctgattga gattgtttaa gcaattcacg tagcctctga cctcttgcta 25021 actgattctg agtagcttta tcaagatcag acgcaaattg tgaaaaagct tctaactctg 25081 cgaattgtgc caactctaat tttaatttgc cagctacttg tttcatggcc tttatctgag 25141 ccgccgaacc tactctagat acagaaattc ccacattgat tgctggtcga attccagcat 25201 tgaatagatc tgctgataga aatatctgtc catcggtgat agaaatgaca ttagtaggga 25261 tataggctga gacatcaccg gcttgggttt caacgatggg taaagcagtc atacttcctt 25321 cacccaattg gatactcaat ttagctgctc tttccaaaag acgagaatgt aaataaaaaa 25381 catctcccgg aaacgcttct cgacctggcg gtctacgtaa caataaagac atttgcctat 25441 aagcttgcgc ttgcttcgaa agatcatcat aaataatcaa ggtatgcttt ttgcgataca 25501 taaaatattc agctagagct gccccagtat aaggagcaag gtactgtaaa gtagcgggag 25561 aattggcagc ttctgctact acaatggtat agtccatagc accgcgatcc tgaaaattat 25621 ttacaacttg agctacggag gaagcttttt gaccaatagc gacgtagaca catataacat 25681 tttgaccttt ttgattcaga attgtatctg tcgctactgc tgttttacct gtttgtctgt 25741 ctccaattat taattcccgt tgaccgcgcc ctatagggat catagaatca atagcaatta 25801 gacctgtttg taaaggttca tatacagaac gtctagaaat aatccccggg gcgggagatt 25861 caattagtct agattcagaa gctgagattt gaccctttcc atcaatgggc tgagccaaag 25921 catttactac acggcctaag aaagaatcac tcactggtat ttgggcaatc tttcccgttg 25981 cttttacaga accaccctct tgtatggata aaccatcacc catcaataca actccaacat 26041 tatcagattc taaattcaga gcaatgccta ccgtaccgtc ttcaaattct actaattcac 26101 cagccattac tttattaaga ccataaatcc gagcaatccc atctccgact tgaagtacag 26161 taccaatatt aacaacctta acttctggaa catatccttc aatctgttta cgaataatac 26221 tgctaatctc atcgggtcga atatttatta ccatttattt ttatcaggta tcttattgaa 26281 aagtgagacc tatgaaagct aatcagctgt attttcaatg gccctgagta ggccaatatg 26341 ataatcaatc atgcgcaggt gcaattcgct atccaaccgg ctattgagac tctctagagc 26401 tctttctgaa gcaaggcgag agacttgctg ccgaacttgt tcaattgctc ttcgttcctc 26461 gaaacgtatt gtaaaatttt tactatcttc taatctttta gaatcttcat tggctgcatc 26521 gattagatct cctttctctt tctccatctg cataagcccg ttgacacgga tctcatccgc 26581 ttttgttttc gcttgttgta aacgggtccg agcttgttta agtctctccg tagcctcttt 26641 ataacattct tctgcatcag aaatagtacc taatattgtt cgcttacgat tctctaagag 26701 attaatcaat gaaagtagat tagagactct tttgttttaa ttaaaaaggt ttctgatccc 26761 ttcccaaacc gaacatgcat ctttcgattc attcggcttt cctgcccgta cttcgctatt 26821 tgataagaga atctaatacc ttgacgggcc tgccatcttt attcatatag cttatcttga 26881 atcaggtgaa aataagatca ctccgactct ataatcgccg aatcacggag gctcttccag 26941 acaatgtttc tactactagc aaggtcgctt cttctgaata atatctatgc catataggtt 27001 gtctattcag caaatagaaa gggtaatcca cttttggaag attctattct cttcttggag 27061 aaatgatatt gtcttcaata tgagtgttgt atatcgaaaa aacttccaac catgcttggg 27121 gatctttgta cggtagggga aagaagaccc aagtttttgc ttagacttta agcaattcag 27181 ctttaaccat tattaaggat attcccatat cagtcattga attatagttt ttatcgattg 27241 tacgaaatgc tctagtcctt acacagtgtt atattattca attgcaagta attcgttagg 27301 attttgcatt tcctcttgtt caaaatgcaa ctggatgaat ccagtcatct tattcagata 27361 tacgactcgc acacactccc tttccaaaat agaataatat acctagaacc aagatcagat 27421 tgatcaaatt tatctctaag atattcgtat ttgaaccaaa acctgcagca agaacccaag 27481 gacttgaaaa ggaagcaata ttacttatac ttctcatact atctcccctc ctaagaggct 27541 atctaagttt taataaaatt taatctaacc tactaagaaa tcatggatca attgaaatga 27601 gggaagcttt aatttattat aatcgactta atagagaaga gagtgaatta caatcaattg 27661 atttgcttca ctctcttgga aacagaaatc tatcaggaat attaggacaa tagagaaaaa 27721 gacttaattt aataggataa taactctttt attaagaata tctttcctag tccggatcaa 27781 agaaaccctt caattgatga tatttaatcg gaaatgtttg tggaagataa agaggatcca 27841 gagttcttta aatattcttt gatattagtg aatcaaacaa acggatttgc aaatgatgat 27901 gctaaggcta caactagtcc ataaattgtt aaagattcca taaaagctaa acttaacaat 27961 agagtgcctc ggatcttacc ttctgcctct ggttgtcttg caatgccttc tactgcctga 28021 cccgcggcag tgccttgacc gacaccagga ccgatagcac caaggccgac agccaatcca 28081 gcagcaataa cagaagcagc agaaatcaag gggttcgtat taatctcctc ttattttatt 28141 cgggttaatt ggtctgggca taatgaagat tctttactaa atacttgctg aaatagtcat 28201 gtcattggaa taaatgagtt aatctgtttc tatctgtcac agagataaat atttctctat 28261 ccccgtgaga gatcccttga aaaataatta gtgattctag tcattatcta ttcgataata 28321 atcggcttaa agtctaatat tgcaaaataa tctataaata gagaatctta ttacgaatca 28381 agggatttca gagttcattg aaatagatag ataccgttta aatccaatat aattagttcc 28441 ttgagaaaca ataaaccctt tcaaattgat gagttccata cataatttac tctttgtttc 28501 tattttgcaa agcacgagga gtgtaggatt tggatcaaat aataagaatt ctgattaaaa 28561 agaacatcct tttactccca gatggagatg atagtaatgt aatgatggca tctctctttt 28621 tcttagagaa ataagatatt gtatatccta ttatatcaca gaaactcttt ctattcgtca 28681 ccaaaggcat ctgctcaaca atatgattat gacctaacgc ttttttcctc agaaacgact 28741 tatctaatga tgaccttcca tagattctcc tatataagct gcagctaaag ttgcaaaaat 28801 cagagcttga ataccactag taaataatcc taaaagcatc ataggaacag gaacaaataa 28861 gggcactgaa gaaacaagaa cggctactac caattcatca gccaatatat taccaaagag 28921 tcgaaaactg agtgataacg gtttagtaaa atcttctaga atattaatag gtaacaatac 28981 tggagttggt tgaatatact taccaaagta attaagacct ctcttatgga aacctgcata 29041 aaaataggct attgatgtaa gtaaggctaa tgcagcagta gtattgatat catttgtagg 29101 tgcagccaac tctccatgag gtaattgaat gattcgccaa ggaaacagag cacctgacca 29161 attagaaaca aaaataaata aaaatagagt tccaatgaat ggaacccaag gacgatactc 29221 ttcttctccc atctgggtcc tagtcaaatc cctaataaac tcaagagcat attcaataag 29281 attttgacta ctcgttggga cggtttgtaa attcctagta gctgcaacag ataatcctaa 29341 tgatatagca attactaccc aggaagttac aagaacttgt gcatgaactt ggaaatctcc 29401 tatttgccag taaagatgtt aacccacttc cacactcgat acttgatata gagaagaaat 29461 cttattaatg agcagttgtt cgatatccat attacccctt ttgtaaaatt aaccgaaaga 29521 aaaaagattt agacagtata caagaaaatc caatatatca agaaagaaat atcttgtcga 29581 tcgtttctat aatagaatta tacgatattt ctgtttctga gataactctt ttgctacttc 29641 attctttcaa cataattgct aggatgatta ttaaccccgt ttattaattt ccaaagcttc 29701 gactgtgaac gaccttcttg aatagctagt attaatttat ctaaaatcca tcggatagag 29761 gatcttgcat catcattccc tggaatcgga atatctgtga gatctggatc acaatctgta 29821 tcgacaacac agatcgtagg gataccaaga gtaatacatt ctcggatcgc agtagattgt 29881 tcatgttgat cagtaattat gacaatatca ggtagatttg acatatattg aataccactt 29941 agatatcttc ttaatcgaat caattatctt ttcaaagtcg ctgactcttt ctttggaagc 30001 cgatcaaatc ctccggtatc ttcttcattt tgcaaaaatt ggaatctccg aagacatgtt 30061 tctatagtgg accaattagt taacatacca ccaagccatt tctcattgac atagtgacac 30121 cgagattttg ttgcaactga tgcaattata tcggctactg gatactttgt acctactatt 30181 aagaattgtt ttccttcttt tgccgcttta aatactagat tacaggcttc ggataaaaga 30241 cgagcagtct taattagatc gagaatatga acaccttttc gttctgtaaa tatatagggt 30301 gacatcttag gattccattt ctgggtttga tgtccaaaat ggactcccgc ctccatcatt 30361 ccgtctaagt caatgttcca atttttctgt tgcattttct ccttgagtac aaaaagacgc 30421 aaataaaatc cattctaata atgattgtaa gattattaca ttcgattaat caggattatt 30481 gattgataga cattcaagat atttgaataa tataaactct gaaacattat ttatcaatag 30541 cagcttcaaa aaagggtttc ttcaatattt gatgaatcca taatatatta tgggaactcg 30601 gcttctctgc agaaccggga agcataatct tcatttcttg attgaatata ttaggatttt 30661 gcataattga atagaatcct ttcttctctt tctctaaatt gagttgacaa ataacctctt 30721 tactccctgt tccgatagga attatatcac caagaataat gttctctttt aaccctttta 30781 accaatcaat tcgaccccga atagcagctt tagctaatac tcgagtagtt tcttggaaac 30841 tagcctctga tatgaaacta gtagtattaa gagacgccct cgtcattcct agtacaatag 30901 gttcatagaa gattgatttc tttaacacac gattcattcg ttgtgcttgt gataattcaa 30961 taaactctcc tggtaagaag acattagtta ttccgtcttc caaagtaata actttggatg 31021 ttatttgtcg aatgataatt tctaaatgtc tatcagatat ttatactcct tgagaaccat 31081 aaatgtttca tatttaatcg atcaaaacca attgacagtg ttccatactt atcccaacac 31141 tcggaaaatg actccaaaga ttcccaatca atcttgtaat acccttattc cattttcgaa 31201 gaatatcttc cattcgtgtt ggaacgaaac cagtggaacg agattccaat aattgttcaa 31261 cctttgggag accctgaata atatccccag atcttaatct atcatataat aatgttatta 31321 atgtatctcc ttctccaata gtatccccgt gatctttatg aatagttgca ccttgagtta 31381 ctaaataagg tttagctgct cttatcaaga aataatcttt attaatagct ataatctgac 31441 ctgattggga gcaaatacca ttcttatgaa gacatatctt ttcgcttatt aatagtccga 31501 gaatcaaatc tttataaaaa tctgagaaga aagaaatccg tttagataag catctcttta 31561 gaaagatacg tctaggatag tcgaatattg attcatcttc atcaattgga tacctatttc 31621 tatgttctaa tttatatgtt gaaacgctat gagccaagaa atcttttgaa gaagagacta 31681 tattaatcga cattaaagga gaagataaca aggattggga aatacccagg aaatcgccta 31741 ataggcctaa ctctcggtct cttcgtttaa tccagatgag attcaattct ttggaagcag 31801 aatatatcaa tcttcttctc aattttgaag tttcttggtt aggagaagaa gatccatatc 31861 tagaattgga acagggatct cttaactgtt tcttattagc aagattgtct ttcatcgatc 31921 cattactatt agcatcttta tccatcccat tagaatgctt tttctgtgac tgcagattag 31981 aacatgagaa tagattacga aaaagattga aggttgatag tattaaacga gatttatttt 32041 gggtgggcac taaatgaata acaccccgat ccttgagtaa tgacttctta ttctcactat 32101 gcaaattaga atcaacggat acgtgttctt tattaatcat tgattctaga agaaccttat 32161 tattttcttg cttgaacaac gatttgggag atatccttga actaagttga aggaaagtac 32221 taagaatatt attgatcttt atagtagtaa aagataaata cgcctttcgt gaagggtaat 32281 attccggcca atcaactatt aaacaagttt gaactaacgg aatatttgta ccgtttctta 32341 ctctgatttc ctcaccatct ccataaaaga tatatggaaa ggcttgtatc tttaaacatt 32401 cgcgtgtctt tgtagcttga gaaggaggag tagctatctg taccaaagaa tcgttagata 32461 tattgtattc tatgactggc cttattgaga cagagatctt tcttttcctt ttagaagaaa 32521 caagcgattg aacataaacc caaccatcgg atttgagccc acctagaatc agattccctg 32581 gtggaatcag cgtatcacta tgttcagaca tagtaattgt cttttccgga taataaacat 32641 atccaggtaa taatcttatt tcaatactct tttgtatctt tttgattcga actgatccac 32701 tcacttggct aataatattg ggagttaatt gtgtaccggc ttcaacaata ctattgttcg 32761 ccactagtac ggatgaagaa ggttcataag taatatatac ctcttcaggg atcaagaaaa 32821 aattaccttc ttctaagatt ttataccagg atctaaacgt tagtatctca ccattaaaga 32881 ctaattgttt cttttcaata tgttctactc ttagagtacc gtaccttaga attcctgaaa 32941 tactagtttg attctcagga ttttcagaaa tagctagaat ctcattccga cttgagattt 33001 tatcattaga taccaaaaga ttgaaacgtc tagaaggtct ctcgttatat ctttcttctt 33061 tttggttcaa tagtaaacaa gctacatttc ctttctttcg ttctatcttg agagtggaac 33121 aactcaaagt agatactaaa tagcttgagc tattcctaaa ggatctttga ttggttttga 33181 attcgcttat ctcttctggg aaaactctag aatcaataat attcagatct cgctttccta 33241 tcttttgaaa ctcttgttta ttcttatcaa tgctatacgg agatctgaca ctaatcctat 33301 cttgatcctg ataagaaaga gagtcagttt tctcggaacc aacagaagat ccggtaagaa 33361 cccaaatatg gcccgtttta cttgctgtac gaatattact atttatatgg tgagacaagt 33421 aatattcgat tctactccaa tagatctctc cgtctagatt cgggtagata tgcttcttga 33481 ttcgttcttt aaggggaaat tctttagatc gtatttctgc aataatctgt tttgattcga 33541 catattgatt atttcgaatc ataagtagac tttgcgctgg aattacaaat tggtgtgtat 33601 cgtttcgatt cttaatagaa atggataatt gattctcaca aagccaggca ggatgtccgt 33661 gtcgcgtacg tgtggcatat actaaccttt catcgaaact aatgagtcca ctgaacggga 33721 ttcgtacatg ctccgcaata tcacctgtaa atactccacc agtatgaaag gttcttaatg 33781 ttgattgagt tcctggttct ccaattgatt gccccgctat aatacctacg gcttctccta 33841 attctattaa atcatgatag gctagactcc aaccataaca cagctgacaa atccaaaaga 33901 tacttctgca tgtcaaaggg gatcttacat atattggttg tatcgccttt gttaggttac 33961 tcgagagttc attactaatt tcttgattac gcgtagcaat acatctaata tctgaatata 34021 cattgtctgc taacacacgt ccaatcagtc ttttttatga tatcgttcgt atagatcctt 34081 ttcttccgtc aataagattt acagcaatgc tacggatagt ctcacaatct tttccacgca 34141 caacaatatg ttggacaact tctacgagcc gacgtgttag gtatccagca tctgatgtcc 34201 gtactgcagt atccacaact cctttgcgag caccgtaaca agaaataata tattctgtca 34261 gagataaacc ttcgcgcaga ttattccgga taggtagatc aatcacctgc ccccgcgggt 34321 ctgacattaa tcctctcatg ccgactaatt gatggacttg ggatgtactt ccccgggctc 34381 ctgaaaaaga catcatatgg actggatttg tgggatcagt cattgtgaaa ttcggattca 34441 tctcctgcct cagccattca ctcgtagcat accatgtttc gatcaattga cgtaattttt 34501 ctacagcatg caaacctcca taacggtgat actgttctga gacgtacccc tgtctctcta 34561 tatcttgtac aagccatcct ttcgaaggta ctgttaaaag atcatcgatg cccagtgaaa 34621 tagatgctct tgtagcttgt tgaaaaccta agaccttgat ttgatcgaga atattggttg 34681 tatatgcgat tccaaaatga agtgctaacc ttcttaaaag ttgttttatc gaagttctgt 34741 ccatcatttt attgtaagaa agtaattcag ctcgatctgc cattagagaa acctcaattt 34801 ctttattcat tctatggaca ttattaacta gtggattcaa gttattcatt gaataactag 34861 aaaagacttc cttgatcctt attattctcg tttctacaga tagaatcttc agactatgct 34921 attggggtta gtaacgactg gagtccaatc ccaagacgtt tttgaaatgc cttgtattgc 34981 ttctactatt tgttgattga atagaatacg accagcagtt gtaagaatat acaagccaat 35041 tgcattattt tctctatccc ttctaatctg ataactagca tagaactgaa aagagatccc 35101 cgaagattca tattgaaact caataggtaa ttcacgattc ctggaactga tcatgtgtga 35161 atcactcttc catcgaagcc ataaacagct ataaagatca atctgtttca attatttagc 35221 cctaagcacg tcatgataac taacaaaata aggtatcttc ggatggggaa tagaactcct 35281 gtctctcgaa tatctacaag aagagtatct gtttccataa atcccttgct tattatccat 35341 agttaatata tagagaccaa gaagcatgtc ctgggttggt acagagacgg gatctcctgt 35401 agcaggagat aataggtttg tatgggaaaa cattgggaaa cgggcttcag tctgagcttc 35461 tagagataac ggtatatgaa ccgccatctg atctccatca aaatcggcat taaacccccc 35521 acagaccaat ggatgcaacc gaatagctcg tccttctatt agaatcggtt gaaatgcttg 35581 tatacctaat ctgtgtaatg taggtgctcg attcagcaat acggggtgac cttgcataac 35641 ttccgaaaga actttccata tgatcggttc tttcttttga atcatattct tagcagttcg 35701 taaattacga gcgatatggc gtccaattaa gtcatgaatt acaaaagctt ggaaaagctc 35761 catcgacaat tcgcgaggta atccacattg atgtaatgag agagatggac ctactacgat 35821 aacggaacga cctgaataat caacccgctt tccaagcaga ttctcacgga atcgtccttc 35881 ttttccttca ataacttcgg agaaagattt gtagggtctg ttattaatat ctcgtattgg 35941 ctgtccacgt atcccattat caataagggc atctacagct tcttgaataa gtctcttttg 36001 gcagacaatc agcccttctg gtgtaaactc acttctcgat cgaaagtcaa tcagggtatt 36061 attccggtaa attattcttc tatagagttc attaaaatca gaagtgatca attcaccttc 36121 ggatagttcg attattggcc tcaactcagg tggaagaact ggtaagatat ctaggaccat 36181 ccattctggt cttatgtttg tccgtaggaa atttttagct aatcttattc gtctcactaa 36241 aagatttttt cttctttgaa ttgcctgatc ttcccattca ttttctgtcg atccttgctt 36301 tgataatatt tgccattccg aatatgcaca atctataata tcttgtagat tcaagctagc 36361 taagcattct ttaatagcat cccctccagt agcaatttct cttccttgaa gagtctcaaa 36421 gcttcgagta gaaaaaaaga ttggaagaat attcttccat gattgttctt cgtaatcgaa 36481 caaacctcgc aatcgtaata gggtgggttt ctcagctaca ggcctagcaa gaaagagatt 36541 gggataggtt gaacgtacgc cccccccctt agaatcggac atgagtgttt tctctcatac 36601 ggctcaaata gctttatcaa aatacgagac tacaatagta tagaactagg ttgtagtaag 36661 actttaggga tcaaatagct attcattact caagtgatag attaataata ttcttttctt 36721 gggtaatctc acttccttct ttaatatgat atgtcctaaa aaggatattt caattctaat 36781 tgtagagtta ttaaaaggtt ttttcttgtt tttaattcca ctattactct tctttaggtt 36841 tctattccac ttcgatggtt ctgacagtat ttgaaacata tttgcgatga atgaagttcc 36901 ataaccatat ataattgcct ctatatctaa tataatagag aagaaatctt gtcaagaagc 36961 acttgactcc cctctcaatc gtattacaat attgggattc ttattacgaa gcctaattga 37021 tagattcata tgaattaacc ctggggaaga atactctttg agtatatata atatatctac 37081 tcttctctta gtgcttctct tcccgagcta catgatactt gtcatggagt atgtcgggat 37141 cagaggcatc ccaccattac cggatgggat gatagatgct aatcaatcta tattaagtgg 37201 aacatatgat caagtcgcgc atcgcaatat actaggcttt ctaattcttt aacaggtcta 37261 gcaagaagat ttccaatgta actaggaagt cgttttaaaa accatacatg agctactgga 37321 catgctaatc ttatatatcc cattcgatgt ctccgaactc gggaatcggt gaattcgacg 37381 ccacaatgtt tacgaaaatt tgaatactct tctctattat caaaagatcg atagtttcca 37441 caagcacaga ctccactctc taccggccca gatattcttt cacagaacaa accatctctc 37501 tctggtttat gagttttata atgcaatgta taaggttgag ttacttgtcc aacgatttct 37561 ccattaggta gttgtctttc ggaccaacta cgaatctgtt caggcgaggc caattcaatt 37621 caaagttgtt gatagctatt ttgatgaatc ataatatcaa aatccttgat ctttttttag 37681 taaaaaaaaa ctaaagtgat tgagtatctt taccctctcg tctcaagtct acgccaaata 37741 tcaatttatg atcgagattc aagcccatag atcgtaattc tcgaactagc aatcgaaaag 37801 attccggagc agtatcgggt ttagatatag gttttccagt tataatagaa ctaagtactt 37861 gataacgagc ctgaatatga tcagatttaa tagtaagcat ttcttgcaat atgtaagata 37921 caccaagacc ttccaaagcc cagacttcca tttctccgac tcgttgtccc ccctgtctag 37981 atctcccttt caagggttgt tgtgtaacaa gtgcataagg gccagtggat catgcatgaa 38041 tcttatcatc aacttggtgg atcaatttca ttatataagc ctttccaatc gtaactggtt 38101 gttcaaaaac atcaccagtt cgcccatcga ttaaccgact ctttccagga tgatcaactt 38161 caaataacca tgggctggat atccttatac ttgctttaca tagttcagaa aatactaact 38221 ttctagaagc ttgtcgttca aatctctcgt caaaaggtgt tactctataa tggatcttca 38281 agaattcccc ggctaatcca agcaaacatt caagaatctg acctacattc attcgggaag 38341 gtactcctaa aggacttaat atcatatcta ctgatgtacc atcttgtaga taaggcatat 38401 cctgccgagg tagaatcttt gatataatac ccttattacc atgccgtccg gctatcttat 38461 cacccacttg tatcttacgt ttctgtaaga tatatatgtg aataatatct gaatcatttg 38521 tcggattctc ttcgggataa atccatctaa tatcaatgac tcgacccctt ccaccaatag 38581 ggactcttaa acaactctct tttgcgttaa ccacttgaat accaaagata gctcttaata 38641 atcttccctc tggagcacgt aatagatccc ctccttcttg aggcgttaat ttacctacta 38701 gaatatctcc tgcttctacc caagaccctg attctacaag cccattatcg tccaaatgac 38761 agagtacata actatctaat tgaggtattt ctttagttat tctttcagga ccctgattcg 38821 ttatacgaac ctcagcttcg tgccttttta tatgaaaaga tgtgtaaata tcttcatata 38881 tcaaacgttc gctaatcagt actgcatctt caaaattata tccctcccac ggcatatagg 38941 ctactatgat atttctaccc aaagctagtt cgcccccaac ggttgctgct ccatctgcta 39001 agatttctcc gcttctcagg agttctttat taattactaa aggcttctga tgcatgcaag 39061 tattattatt agaacgttca tagactctta atttcttatc tatgatttct ttgttagaaa 39121 ataatgtaat tctttcgcca tcgagatagt caattcatcc ttcttgttcg gatacaacta 39181 catttcccga atctagggct acttgaccct ctagtcccgt tcctacaatg cacctctcag 39241 gcttaaaaag cggaaccgct tggcgttgca tgttggaacc catcaaagca cggtttgcat 39301 cattatgttc aataaacgga ataatagaag ctccaataga gaaatattgt agaggatgaa 39361 tacttcggaa atccatttgt tgccaagtaa cagttaaaaa ctcttgtcta tagcgaacca 39421 gtataagttc tttattgtaa ttcctccaat ctgatattaa caagtttcca gttgctatgc 39481 gagaataatc atcctcagca gaggatagat caactatttg ctctttgcta gatattttag 39541 ataccctaaa gaagggactc tgcagggatc cagaattgtt aacctgtgaa caaatagcta 39601 atgatgagat gagaccagca ttgatgcctt cggatgtttc gataggacag atacgcccat 39661 attgactaaa atgaatatca cgagcttgaa aattggcagt tcttcgtgtc aatcctcccg 39721 ggcctaaagc acttaacctt cgtttgtgaa cgatttctgc tagtggattg gtctgatcta 39781 gaaattgtga caaaggatac gaaccaaaaa attcgctaaa agttgtcatt aatgggacag 39841 aagttatcaa tcctttaaga gttagtgggc gtctacgttt aatagcttta tgaatatttt 39901 atcggatcga aatctctaaa cgatttaaag ctaaaattaa ctgttctgtt aagagatctg 39961 ttatggatcg aacgcgtcga ttcttgagat gatctatatc atcaagacta cctattccat 40021 agctgatttc tattgaataa tctgcggcag ttaatatatc ttagggtaat agaaaatgtt 40081 ccgtttcagg tacgtcaaga ttgagtttga tgtttaaatt tcgtcaaccg attggtccta 40141 attcacacct ctgttagaag aatctcttct ataattcctt taatatggat tctgaaaata 40201 ctatatctaa tcctgcggag tataaatgtt tataaagttc caatatagca tcttctggtg 40261 attcaaccaa ttctctttgt ttttttaata gattgagaaa aatcttagga taacgcacgt 40321 tttgaagaat atctcttatt gttaatccca tagataataa cagaatcaga atggatatct 40381 tccgtttctt gctaatgcga atccatattc tatttcttct atccatttct aatttaaatc 40441 ttccccccca atccgaaatt attgtgctag tatatgtaga aactccttta tgatccagtt 40501 catggttata atagatgcca ggactcctca atatttgact aattaagact ctagcaattc 40561 catttattat aaacgtccct tgggaattca tagaaggaat agatcccaga agtacaatct 40621 gtttttggac cgttctgtct ctcttccaag ttaattggat cggtacatac agatcgaacg 40681 aataggtaat aaattgatat atagcatctg tctcttcgat cgaaggtttt gccaattgat 40741 actgttcact aattgattca agctcaactt ctttatccgc atcttcgatc tttggaaaat 40801 tatcaagttc ttcaaatagt ctctcatgaa caaaacgatt gaatccctct aattggattc 40861 ttccaagttc cggcaataca aacatctttc catttcctct tcatttttta gtgattggat 40921 ttccgttttc atgagaaaac tatgtatatc taattttttt ttagtgattg ttaaaaataa 40981 tcacaaacga agagaatcag atatagacta tcaaagacaa atagaatgta aaacaacgga 41041 atgattcttt tcttagtaat ttgtttgccg agagataaca tgtgctctaa atcagatctg 41101 agaagagacc actagattct aaataggagc aaatcatctt gccaataatc ttattcaaga 41161 ttataattat tgttgagttt cttatcttat aaagatctat tgaatatcta tcatttgcag 41221 gattcgatat aaacagatga aacagaggcg atatagtcaa gtggtaagac agaggattgc 41281 aaatccttca tcccccagtt cgaatctggg tatcgcctac caaatctagt tgagagagtc 41341 ttgggattgg ctactgtgaa agattcagac acaaaatgag atgctctata ttcttttatg 41401 gcctatcacg gtaaatggat ctagatatga cacaaatgga caatcccaat catattatac 41461 catagattga ggaatagaag aattcctttt tccaattttt aggataaagt cttagtaaca 41521 ttgaaaaaga agaatacaaa gggatatata cattctcact gctattctgg gtattataag 41581 gaataaggat aagagctttc ttaggtacag tacatacaaa atagttgagt ctgtgtaact 41641 aaatcaatta tattagaagc ataattaggt taaaggatgg gaatcacaat tatggatata 41701 gttagtattg cttgggctgc ttcgatggtt attttcacat tctctctttc actcgtagtt 41761 tggggaagga gtggattata atcgataatc attcactctc tccttaatat tctactgggg 41821 aatgcgaatc actgggcttt acctcaatga ttcgatcccc ttcttacttc ttcatataaa 41881 acatatagag tataaggaac ccttcgtttt ttctgtaaga atattcttct ctttactctt 41941 cctaatctca aatctttgta agaatcttct tgaataagtg gattgaacca ataaatcttt 42001 gaacaattag tattcaatag ttgtgtttgc tggttagtct tttgagatat ctcaattctt 42061 ttagtgcgta gatacaaatt ttcgataaca agaaatattg caattccact cagaagtgtt 42121 tcagataata gtaaaatagg atctaaacgc caaccttgaa agataagaat ccctccgcat 42181 aataacccaa tggaagaaag aaagaaatca taatattggg atagattgag atactactgg 42241 tattctgccc cccctaaaaa ccatatgtga ttctttcatc tcgtatggct taagtgagaa 42301 aattgataga atcacagaga agatctaata gttgactaat ctctcacctt aatccacgtc 42361 ggtttttttg tttgtttaaa cttcctggat tctctttcac tagattaaaa tcatgaatgg 42421 atagataatt actatctagt tgattcattt gactaataag gatacaaaca gaacagatct 42481 ccgttttttt tctttttaat ctagtttttt ctttatatac ttgatgcact ttgctgtgaa 42541 atacttagat cgttagacct atgttcaact ccataggctc tttatttata ggcctatata 42601 tataaatatc tcttctctag aggaaagaga ttcaatctgg aataatctaa attaaaggaa 42661 ataccttgct tagaagaacc aatacatacc cttaacttag cttttgaagt ataaataaac 42721 atattgaaga gatctgatca tcgattagtt tcttttagaa ctgattatct ttgatactaa 42781 ttcaagtttc ctttcttttc aataaagata ggttgaggtt tataagaata tcatacaagt 42841 atatttgaga tcacacctct agatacatta ggttcccttt ttccaagggc gtataagaag 42901 atacctatca ctactagccc gacgccaatt aatgctccgg gaccaaattc catagaaatc 42961 ataagaaata tagagaggat atatctatcg attgatagat atatctagat ttgtatgtta 43021 atctttgttt atatttatcg atatagttat tcgaatggaa atgagaaaag ctgccggatc 43081 taaatcaaga aggaaacata ataacgataa atatgtttac taacgattag gttattgatt 43141 cgtttggaat ggaaaccaat aatgttctgt tcttttttct cttacggata tttcagttgc 43201 atatgcttga gtatttcttt cttacgaata gaaatagaaa gagaacaaac tgagcttcaa 43261 tttagttgtt ttgagcagct gtttgaatat aaagaataag gagagaagct gtaggaatta 43321 gaataaagag tgcagtagct atgaatgcga gaatatttac ttccatatta acatattaat 43381 agttcgaatt gattagagaa taatttgagc aatctcatcc gaagagacta ttggactcta 43441 ttaaaaatca ttaaactcga ctgagatatg tccaattgat ctattgatat gagatcaata 43501 agtcttagtc tagttaaatc atcgatatca actatatagt atatcatgta tatcatgaaa 43561 ggaaagctca agaatagttg aattccgata tttttcaagg agaatctatt ctttggtttc 43621 ttcttttctc tcttcttaaa aagatgctac tgtgcttatg cttctctttg acatcagaga 43681 ttataaacat tgttactaat aattaacaga tttaattcta tattgctcgg atttttctat 43741 ctctaattga ttactagatc atctttttgt aaggaatctt aaccaaaaag aagaaacaca 43801 acgaagcaac ccgcatctgc tcagaattgg tatagaatcg aattgttgct ttgccaattg 43861 aagctgattt aagagaccct ccccctcacg ccggggaggg ggttcaaaac agattcgaag 43921 actaaccatt aaatatttga cattgaaatg atagatatat agactctata tactgggatt 43981 gtagttcaat tggttagagc gccgccctgt caaggcggaa gttgcgggtt cgagacccgt 44041 caatcccgct tatcttgaat acgtggaaag agattaaaca gtatatagga gttgaatcaa 44101 ttctctttag agatttagat tctcgttgtg gttgttgggt cgagctggat ttgaaccagc 44161 gtaggcatcg ccagcggatt tacagtccgc ccccattaac cactcgagca tcgacccaac 44221 catcgaaata taaatactcg acagatagat agaaattaat tgatattgag attctagggt 44281 tttttgaacg gatttagagt ccgtctccga tcgaacggat tctgggtttg tctcccaacg 44341 aacggattta gagtccgtct cctgtcaaac agatttagag tctgtttctc gttaaacgga 44401 tttacagtcc gtttctcatt acagagtaca gagaatgaat aataggaatt ggaatataag 44461 aatggaaaga cctgttccac tacgtctgga attcttctgt agaggaacta cccccagggg 44521 aattcgaatc cccgtcggct ccttgaaaga gagatgtcct aggcctctag acgatggggg 44581 ccgtttctct tactaaagag taggctatta ctcataaatt gtcaatctga tctagttgaa 44641 taatatccaa acatttgatt gattcacaaa tcgattcaat ttttcaagaa ccttgaggat 44701 tctatctaat caacgaataa aagtgagatt tctgatccat ctctcgtttt tggactgccc 44761 atatatatta ttacacaata tatctttaaa tgcaatagat cttttaaaat atcttgtgta 44821 tggattcaag aaatggataa aaatgccgag atactaagaa tgattaggaa atctatctgt 44881 tacaatcttt caagatagta tgaaatagct aagaaacaga atccaattaa tatcttctct 44941 ttcgttttaa gaatccagtc tttatcctta gaacttcaaa caataaaaat gaattattac 45001 tagtacttat gtttcttcgc gctattctga ttatagaaaa ttattctttc tatgtctagt 45061 atcctttagt atattataga gtcctcgctc tctttacaaa ttaggaatgt aaaattcgaa 45121 tcaaatcaca aattgtttag ttctggctct gtgctgtaat ttgtaagacc taccaatcac 45181 aatattcctt ctctctctat tgatatatcc accaatatgc tttgcttgaa gtagaaatag 45241 atggataggt atcggatgat tggcggatag cgggaatcga acccgcgtct tctccttggc 45301 aaagagatat tttaccattc aactatatcc gcattatata ctatatcata ttgttccata 45361 ttcggaatat agttctattg atctcatata atcaatatat acaatctgtc ttttttttct 45421 ggctgtctca tcaattgaga gttgataatc tctcccctcc cacttaaaag aattaaaaga 45481 atgcagattc gattctgaat attatgacta tcattattga tatctttgga ataaggaatc 45541 gcggggaggg gaaactgtaa cgttcaatac attgatttca ttaatttcta agatatgaaa 45601 gaattaagta tacctactaa gaagactaat ccgatccata aagaagctcc tgaaaagaca 45661 atattcttat tgctcgacca accatcagga gaagcaagta caacaggtac accaattact 45721 agtagaaaag aaatagcaat tagtgcaaat agagctaatt ggaaagcaat agtcatgatt 45781 tattgtatat attgtatatc cagaatggaa gatttgtacc atccgatccc catccaatga 45841 gattcttact ttaaatttga aaggattgta taccggatca ataccttctt agtattccaa 45901 gatccaatgt atccaaattt cttgatcaat tatcaaggaa agatgaatat ctcatcagga 45961 tgatcatcga taccggtttc atagtaaatg aaatattcaa aaatctttat caatttgtat 46021 cttccctaat agatgtcgtt tttgatacaa tatatgatat agatggatca agagccataa 46081 gatttctatt atttaaatat cctatattct ctaatattga aagaagcagt ttaatccatt 46141 gaggagagat ggtcgagtgg tttatggctc cagtcttgaa aactggcata gtttatctat 46201 cgagggttcg aatccctctc tctccttatt acttatttag tatcagatct cgataccaaa 46261 aaggcatgaa tggattacat gaataataga ttcctatctt atattatatt agtacttagc 46321 atttagaaaa caaagtcttt ctgactaata aagattgatt agatcaaata gcaatttgaa 46381 gatactctta tatcttcctt tgcttgtgtc acatgctgta ggttgaagct taagttccat 46441 tctaatcttt ggaattcgaa gaataataaa caattatgat ctatcaaaaa aaattagaaa 46501 tagttagaac acggatacaa ttattctatt gaggggcggg ggttattaga atcacttcat 46561 taatcatccc ccgcccatcc atctaagggg tggttatgca gatatgctat catacataac 46621 tcctagagga ttgaatcatt tttttctgaa gaatcaaaga gattaattcg aatctttctt 46681 tctctttttt aggaaattga tctgaatata taaggaattt tggtgttcca aaaccttatt 46741 attgattagg attctagaga gctaaatagt tagtatcgct tctttgattg aaatgtgcaa 46801 gcaagggttt actgatcaga tacgtacata ctgatagttt tctaaccaag ctgtatctga 46861 tgttgattgt taaacatatc aaatctatca caaaatcttt actaagattc actaggagat 46921 tgaggaagga cattcacctt ctgtctaata gaataacaag ataagatggc aagggcattc 46981 ttacaattat tctttgaata atccttgtta attcagaggt gtcataaaaa gcacaggttc 47041 ggtatcacga tcaattccct tctcaaaccc agctgcagct gcacgggctc ttcctgcatg 47101 ccatagatga cccacaaaaa agaagaatcc cagaacgaaa tgagaagttg acaaccagct 47161 tcggggagat acatagttga cagcatttat ctcggtagct actcctccta ctgaatttaa 47221 ggaacctaaa ggtgcatgag tcatatactc tgcggaacgc cgttcttgcc acggttgtat 47281 atctctcttt aatttgttca aatctaatcc attagggcct ctcagaggtt ctaaccatgg 47341 agcacgaaga tcccagaagc gcattgtttc gcctccaaat atgatttctc ccgttgggga 47401 acgcattaga tatttaccca agccagtagg tccttgagca gaacctacat tagcgccaag 47461 acgttggtct ctaacaagaa aagtaaaagc ttgagcttgg gaagactctg gaccagtggg 47521 accatagaat tcgctaggat atgctgtatt attgaaccaa acaaagcaac aagcggtgac 47581 accgaaaata gcaagagccc ctaggctata ggacaaataa gcttctccgg accatacgaa 47641 agcacgacga gcccaagcaa aaggtttggt taatatatgc cagatccctc cgaaaataca 47701 aatagatcct aaccagacat gtccgccaat aatatcttct aaattatcta cacttacaat 47761 ccacccttct ccaccaaaag gagattttag gagataacca aagataacac tagggtttag 47821 agtaagattc gtgattcttc ttacatcgcc acctccagga gcccatgtat cgtataagcc 47881 tccgaaatac agcgctttga atactaagag aaaagcacca gcccctaaca agatcagatg 47941 aatacccaga atagtagtca tcttgttctt atctttccag acataaccaa agaatggaaa 48001 ggactcttct agagtctcgg gtccaataag agcatgataa atacctccga aacctagaac 48061 cgcagaagag atcagatgaa gcactccgga aacaaagtag gggaaagtat ctattatttc 48121 cccacccgga cctactcccc aacccaaagt agctagatga ggaagtagaa tcagcccttg 48181 ttcatacatg ggcttctcag gaacaaaatg ggctacttca tacagattca tagcccccgc 48241 ccaaaatacg atcaagccag catgagctac atgagcacca agtagtttac cagatagatt 48301 tataagtcga gcattaccag cccaccaagc gaaacctgtg gtttcctgat ctcgaccacc 48361 cagagataga gttccattaa agagcgtttc cacggggtag aacctcctcg gggaatacaa 48421 ggttttcatg aggctgatct tgagctgcca tccaagcacg aataccttca tttaataaga 48481 tattctttgt gtagaaagtc tcaaactcag gatcttccgc cgcacgaatt tcttgagaga 48541 caaaatcata cgcacgtaga ttcaaagcaa gaccaaccac tccaatggca ctcatccata 48601 acccagttac tggaacaaat agcataaaga aatgtaacca acgcttatta gaaaaagcaa 48661 caccaaatat ttgagaccag aaacgattag cagtaaccat tgaataagtc tcttcattct 48721 gggttgggtt gaaagcccga aatgtatttg caccatcacc atcttcaaat atagtgtttt 48781 ctactgtagc accatgaatg gcggataaaa gagcagcacc gaggacccca gcaactccca 48841 tcatatgaaa tgggtttaaa gtccaattat gaaaaccctg aaaaaagaga atgaatcgaa 48901 agataccagc tacaccaaaa ctgggggcaa agaaccaccc agattgtccc aaaggataaa 48961 ttaagaatac cgaaacaaaa acagcaatag gagcagagaa tgctatcgca ttatagggac 49021 gtaattggac ggacctagca agttcgaatt ggcgtagcat gaaacctatc aaagcaaaag 49081 agccatgaaa agcaacaaaa gtccatagac ctcccaattg acaccaacgg gtgaaatctc 49141 cttgggcttc cggaccccat aatagaagca acgaatgtga cagactatta gcaggggtag 49201 aaaccgcagc agtcaagaag ttacaacctt ccaaataaga gctagctaaa ccatgagtat 49261 accatgaagt cacaaaggtt gtaccagtaa accaaccacc tagagcaaaa taagcagtag 49321 gaaaaagtaa tagaccagac caacccacga aaacaaaacg atctcttctt agccaatcgt 49381 ccatactatc aaataaatct tttggctctt tggaagattt tccaatggca atggtcatag 49441 tagttctcct tcctattcaa ccttcataac tcaactgttt ttaggtgtcc tttcattcca 49501 tgacatgata ttccaagatt tcaaacaata ttgaactatc agatcattaa cccttctgaa 49561 gaatcgtttt ccagagcctt tattttattg acttcccagc atgtaataat agtgaaagat 49621 agagttcttt ctctctttct ctccactact atcctctgaa ttgccgagtg tagagtcttg 49681 ggctgagata agattattct ttactgttat tcttcaaaaa gtaggtaaac ctatctcttc 49741 ttctgagatt ttatcagcct gtttgctaca ataatgaata tgcttctggg acctgatccc 49801 aattccctat ttattattaa ataattagtg gcatgctttg aactttctag agtattggca 49861 ctattttgaa acaagcattc tttccaatac aagaaaagag agatgacaaa aacagagcgc 49921 tatctgcaag aatttctaga atcgatggat aaatagaaaa aaacacacat caaattaaaa 49981 ttgaaaaaat agaacacctt atagtttcta tatagatccg atccatatag ttacttgatt 50041 catctctctc ttaatcgcct ttcaccattt attctaccaa tctttaataa aatttgaaag 50101 ctttgtttct agtcttgctt gaatcattgt gatgaataat catcaaaaaa aaaagaagga 50161 tttctagttg gattaaggaa tgattcatcg ataggttaat tgattgagaa ggatataagt 50221 ctgttttgac tactagctac aaagcagaat agaggttaga gcgttaccca aaagattata 50281 tcaagtcttc agaatcaaga agcaaggatt taccaaaata tggattgtct taggaattcg 50341 tcattatctt aggaaaattc ttctgttctg tagataaata gattgatggt gagaatccgt 50401 atctgaattc ctatactaat aataactaaa ttgttcatat ttatatgagt tattttcact 50461 tatttaatta gcgctgattc tatggataac actttctctt tatcttatcg aaaattccgc 50521 ttagttatat agagttttta tcaacacaaa caaaaaagag agaaagaatg gtgggcagga 50581 tattcaagat taataataaa taagaatgat ctgatttaat tagaaggaat ccacttaaga 50641 ctatatgaga tgagctcctc tgctagatct aaatatctca atctattaat ctttgttatt 50701 aataaaagca tgcatggagt agtctttatt ctatttcctt tcatttaaca tagaaatata 50761 ataatatttt tttatactaa actaaaggta gacaaattga ttttcatgtt cttaagcaaa 50821 cagcccttta tcggatttga accgatgact tacgccttac catggcggta ctctaccact 50881 gagttaaaag ggcttctgaa tgatattgaa gggtataaaa tataatataa aacatagttt 50941 tgtttacaag actatgtctc ttcttcaaat cgattcttta acttgtgtat ttcaatctct 51001 ggttcttttt gtatatctaa cccaaggggc gatttttaaa caaagattaa ttcaaagaaa 51061 atagttagat tgttaatcga gctctcttct acactcttgt ttcaaacaat aatcagaata 51121 tttactagta aatatctatt cttttaaaaa caagctgtag attatccatt atcccccaaa 51181 ccagcttata gttgaattat tgacaagata aatagttagg atagagatgc agcaaagagt 51241 agacaatttg atcaaatcaa aataatacca tctcttctta tgtggatatt attttgcgga 51301 gacaggattt gaacccgtga cctcaaggtt atgagccttg cgagctacca agctgctcta 51361 ctccgcgtta ttaatccatt caatataatt attatattac ctttgaataa ttacattgaa 51421 tgcgaagaga ttcttagaat atgatcctgg actatatctt atccatttct acgaaaaaga 51481 ttaatagaat cctactctac caactcgatt ttattacccc gggcaacaaa caagcatgtg 51541 ccatttcacg taatacatgt ctagataaac caaaatctcg ataattagac ctaggtcgtc 51601 cagttaggga acaacgatta tgaaggcggg tgggtgcact gttacgtggg aaagattgta 51661 atcttttaga aatctccaat tttttctgta ttgacgaact ttctttcctc tctcgtttta 51721 aagattgacg aatggaatga tattttttca ccaatttctg ttttctcttc tctttctcga 51781 tgagactctt ctttgccatg attaactgtc atcgatacta aaatcttatt ttaggctaag 51841 gtataattta aaaatagtat ggaatattat atgattgaat gcttattatt gataatccat 51901 ttatcagaaa gagattggac ttgtaagtat gttgaaccct accataggct tgaacgccaa 51961 ggctaggtcc aacatactat ttgtttctag ttgaattaga attcatccaa atttacctga 52021 tgtagatgct attaaaaacg ctgcatacgt aaatatataa cctacagaga aatgagttaa 52081 tcctactaat cgggcttgaa caatagaaag agccactggt ttatctttcc agcggatgag 52141 gttagccaac ggtgtacgct catgagccca agctagagtt tctataagtt cctgccaata 52201 accgcgccaa gagatgagaa acataaatcc aatagcccaa acgagatgtc caaatagaaa 52261 catccaagcc caaacagata aactgttcat accgaatgga ttataaccgt taataagttg 52321 tgaagaattt aaccataaat aatccctcaa ccatcccatc agataagtag aggattcatt 52381 gaattgagcc acgtttcctt gccatagggt gatatgtttc cagtgccaat agaaggtaac 52441 ccacccaata gtatttaaca tccaaaacac tgctaaatag aaagcatccc aacctgaaat 52501 atcgcaggta ccacctctgc ctggaccatc gcaagggaaa ctataaccaa attctttctt 52561 atctggcatt aatttagagc cacgtgcatc taatgcccct ttcactagga tcaatgttgt 52621 tgtatgtaaa ccgagagcaa tggcatgatg aaccaagaaa tctccgggtc caatcgtcag 52681 gaataatgag ttgctactat tattaatagc atctaaccaa ccaggtaacc atagactctg 52741 accagcatta aaagctggac tatctgctga agatagaagt acatcaaaac catataacgc 52801 tttaccatga gtcgcttgta tccattgagc aaatacaggt tcaatgagaa tctgtttctc 52861 gggggtcccg aaagctaaca tgacatcgtt atgaacatac aatcctaaag tatggaatcc 52921 taaaaataaa ctagcccaac ttaggtgggc aattattgct tctttatgct ccaacattct 52981 tgccagaaca ttgtccttat tttgttccgg attataatct ctaataaaga atatagctcc 53041 atgagcaaag gcccctgtca taataaaccc agcaatatat tgatgatgcg tgtatagtgc 53101 agcttgggtc gtaaaatcct gtgctataaa agcataagcg ggtagagaat acatgtgttg 53161 tgccactaaa gaagtaatca ctcctagagc agctaacgct aatcctaatt gaaaatgaag 53221 agaattatta attgtatcat aaagaccttt gtgtccacgc cccaatctac ctcctggagg 53281 aatatgagct tctaagatct cttttatact atgtccaatc ccaaaattgg tcctatacat 53341 atgaccggca attataaaca gaactgcaat tgccaaatga tgatgagcca tatccgttag 53401 ccacaaactc tgagtttgtg gatgaaatcc gcctaagaag gttagaatag cagttcctgc 53461 tccttgagta ctattgaaca catgattacc agaatcagga ttctgtgcat agatactcca 53521 ctggcccgag aagaatggtc ctaatccctg agggtgcggc agcacagtga gtagattatt 53581 ccatcttaca tgctcacctc tagattcggg aatagagaca tgaatcaagt gtcctgccca 53641 agctaaagaa ctaactccaa agagtcctga aagatgatga ttaaggcgag attcagcatt 53701 cttgaaccac gagatacttg gtttccattt aggttgtaaa tgtaaccaac ccgctattaa 53761 gaataaagca gaaattacta ataagaagat agctccaata tagaggtctt gattagtgcg 53821 taaaccaatt gtataccacc attgatatac accagaataa gcaatattaa ccggacctga 53881 agccccccct cgggtataag cttccacggc tggttgacca aaatgaggat cccaaatagc 53941 atgagcaata ggtcttatat gcgatggatc ttggacccaa ggctcaaagt tcccttgcca 54001 agccacgtga aatagattcc cagaagtcca tagaaaaata atagccaatt gaccagaatg 54061 agaagcaaat attctttgat aaagacgttc ttcggtaata tcgtcgtgac tttcaaaatc 54121 atgcgcagta gctataccga accagatacg acgagtagtt gggtcttgag atagaccctg 54181 gctgaacttc ggaaatcttg atgccatagt gcttttcaaa tcctcctagc cattatccta 54241 ctgcaatgat tctcgctagg aagaatgccc atgttgtggc gattccaccc agaagataat 54301 gagctacccc tacagcacgt ccctgtacaa tactcagagc cctaggctgg atagcaggag 54361 caactttcaa tttattatgg gcccaaacga tagattcaat taattcctgc cagtatccac 54421 gaccactaaa taagaacatt agactaaaag cccaaacaaa atgagcccct aagaataaga 54481 gaccatatgc agataatgaa gaaccatatg attgaatgac ctgggaagct tgtgcccaca 54541 agaagtcacg tagccatcca ttaatggttg ttgaactttg tgcaaaattt cctccagtta 54601 tatggtttat aactccctga tcacttatac tgccccatac atcagattgc atcttccaac 54661 tgaaatggaa tataactatt gagatagaat tatacatcca aaataaacct agaaagacgt 54721 gatcccaagc agatacctga caagtacctc ctcgaccggg accgtcacaa gggaaacgaa 54781 aacccagatt tgccttatcg ggtattaaac gagagctccg tgcaaacaaa acacctttga 54841 gtaatattag tacagtcaca tggattgtaa aagcatgaat atgatgtacc aagaaatctg 54901 cagtacctaa tgggattggt gacaacgcaa ctttaccgcc cactgctact aagttattac 54961 ccccccaggt tagactagta cctgttattg cattaggtgc agttaaggca ggagcagagg 55021 catgagtatt ttgtatccat tgagcaaaaa caggttgtaa ttgaatagcc gtatctgaaa 55081 acatatcttg agggcgtccc agagcactca tagtatcatt atgaatatat aagccaaagc 55141 tatgaaaacc taagaagatg cacacccagt taagatgtga gattattgca tcacgatgtc 55201 gaatgacacg atctaataaa ttgttgtatt gagtagtcgg atcataatcc cttaccataa 55261 aaatagctgc atgcgcagcc gcgccaacta ctagaaatcc accaatccac atatgatgtg 55321 taaacaaaga taattgggta gcataatcag tagctaaata cggataagga ggcatagcat 55381 acatatgatg agctactata atcgttaaag agcctaacat tgcaagatta atagctagtt 55441 gagcatgcca tgaactagtt aagatctcat aaataccatt gtgaccttca ccagtaaaag 55501 gacctttatg cgcttcgaga atctctttcg tactatgtcc aataccccaa ttggttctat 55561 acatatggcc agctatcaaa aaaagaaccg caattgctag atgatggtgc gcggtatcag 55621 tcaaccacaa tccccccgtt actggattca atccgcctcg gaaggttaag aaatccgagt 55681 actctgccca attaagggta aagaatgggg ttaatccttt tgcaaagctg ggatacgttt 55741 gagccaaaag atcgcgattt aatatgaatt catgagggag cgggacctct ttaggatcca 55801 ctcctgcatc taataattgg ttaattggta gcgaaacatg cacctgatgt cccgcccatc 55861 ctaaagaacc taatcctaag agacctgcta gatgatggtt caacatggat tccacatctt 55921 ggaaccaagc caattttgga gcagccttgt gatagtggaa ccaaccggca aacaacatca 55981 atcctgcaaa gattaaagca ccaattgcgg tgcagtagag ttgtaattca ttagttattc 56041 cagaggctcg ccaaagctga aaaaatccag acgttatctg tatcccttgg aacccccccc 56101 ccacatcacc attaaggatc tcttgaccaa ctataggcca aactacctga gcgctaggtt 56161 ttatttgagt aggatcattc aaccatgctt catagttaga gaaacgagcc ccatggaaat 56221 acataccgct tagccatata agaataatag ctaattgacc aaaatgggca ctgaatattt 56281 tccgggatat atcttccaga tcattggtat gactatcaaa atcatgagca tcagcatgta 56341 aattccaaat ccaagtagtg gtactaggac ctttggctaa ggttcttgag aaatgtccgg 56401 gttgagccca tttctcaaaa gaaactctta caggatcctt ttctaccata atcttgactt 56461 ctggttccgg tgaacgaata gtcattgagg ttctcctctc ttcggacaaa ggatatcaac 56521 agcctaccaa tagaggataa aagacttcta aaagatagat tttattctag tagaatattc 56581 tttctctccc tgagtttaaa actagaacga ctttagtaga agagtaatcc aagtaatcta 56641 aggtaggcgt ttgatcttat tattacacag gttcatagtg ctttatggac ctaatttgat 56701 tcttcattct ggtagacaga aagaatcgaa tatgcatgaa taagcattga aaagagaaga 56761 gatctacctc ttctctcgga attgaataac ggggggggat caaattatat gtaatcctaa 56821 cgaatagaat ctctttgctc cccgctcctt atcctttttt ttattattta aaacgtcttg 56881 ttacctttaa ccaattctgt gcttcgatat aattgcttgg ggaaagtgct attgcttgtt 56941 tccaatactc agcagcttga tcaaaccagg cttctgaagc ctctggttct ccttgttcga 57001 tggcctgttc tcctcggtcg ggatagatag attcttctaa cctatcctcc cttagaaccg 57061 tatttgagag tttcccacct catacggctc aaatgactat tcatcaattt cttgtctatc 57121 ccggctaaga gaaaaagatt tacggatctt ttactcttat tagcatatac aagaactaag 57181 aagaacaatt attgtaatgg tttccaatcc tattagcatt tttattttat tgtcgcaaga 57241 tagaatcttt tccaccttcc taatccatta aaaagaaata ataatcttct tgagaactaa 57301 ttatcctact ggttcatatg gatccattca tctaaatata gatttccttt aggatttaat 57361 actacaccgt gactcaatag ataatattct cttcaatcaa tcttattaca agaattgatt 57421 gtataacctt ttgggtgagc aaatctcatt gtatcgacct agatcttcaa tgatgaatct 57481 ttacgatgct ctcattctca attcacacta ttatccatgg gatttctagg ccttcatttc 57541 ccccagaaat ctagattatt actagggaga ttacatccca cagcttatga atactcttat 57601 tctttgttat gttaattgaa gaaagtatgt ttgtgggaag cctttcctat cgagtagttc 57661 caaactataa gatcctatag tagagataat cgcacgtaat gacagatcac agccatatta 57721 ttaagagctt gtggaagaaa aggattccgt tccaatgctt ggaaatagta ttccaaagcc 57781 tttgtgtgtt ctccattact agtatgagta agacctatat tgtaaagtat ataactccga 57841 tcataaggat caatctctaa tcgcatagcc ttataataat tctgtaaagc ttctgcatat 57901 tctccttcgg attgggccga catccgttac ggtcgttcat acaatccaaa tcaaatgagc 57961 tccgtttcaa aaccgtactt gagactctca tctcatacgg ctcctcctgc ataatataaa 58021 tagagagaga ataattttca tagattaaac agtttaatta ttactactct cagttatgga 58081 taagcggggg ctagcgttct gttccttgga ccaatatcta accatccttc tagtcaagaa 58141 tcttttagta caaataccta attcattcca atatagtaac aagagtctac ttgtaaaaga 58201 ataaaagact atttgacaat ttctttgttc tcaacgcttt gtaccttaca aggataagct 58261 acttcgacaa ccttcgatga ttgtaagggt atccaaaata caaacgagat gggtgtttgt 58321 tgtcccaatc attcttaaga ttcttcatag aaacgtaata tatggataaa tttaaccata 58381 acgaagcttt ttcattgtcg atttctacag gtagcgcgtc tccattatgc ttaccatgag 58441 tattacaaag gggtaatctg aaccttttgc ttaccaatct gatctactcg aaatcagagt 58501 agtaaaggct gcattagtcg aggatacacg acggaaggac ttgcttcggt ttcaaaacac 58561 accttcacca agcgtgagtc tcttgaaatc ccaatcttct ctatatttta caaacaaaag 58621 gattaagatt gtttattttc tttgcaaatc gcaccatctc tataatgggt aaaagcttct 58681 ctctctctgt tagttgttgg tataatttgt aataaaatat cagctagaat tgtgaaagtt 58741 ctatcaatag aattgtcatt tctctgagat ctaggcataa ttgattagga tcctttcttg 58801 gtccatttga tatgagaaat acaagatcaa aaaaagtaag acgagatata tccaatatgt 58861 ctatttctat ccctcgatcg attaaaagaa agataataat aagctttatg atattctaca 58921 atatcaaggt cgtcttagaa tggattgatg agctcgtaga aatatcaatg attctgtcgg 58981 aaatgattga ttccaatgat caattatctt attgaaacgt attgattgaa agtgatcact 59041 caaatcactc aaaaagaatc aatcaaataa tatgattagg aaacaataat tagcaagatc 59101 tattgaatcg agcttgttga ttattgaatt tcaccgattg tctgattctc tattctttcc 59161 aatcctagac tcccagctcg taagctatcc ctcagattag ttgaataccc gttttagaac 59221 gattcaccat tccaggaaag acaggaaagg tgactgagcg gtttaaagtg tagcactgga 59281 aatgctatgt agactcctgt ttaccgaggg ttcgaatccc tctctttcct tattgtatac 59341 tctctttttt tttcttattc cattctaaaa aaacttctag aatgtggagg attcggaata 59401 aatattctct caatatcaaa atgagatcta ataaataaag aaggatatct tattcaagat 59461 ttaaagatct aatgcttgtt gaaaaaacac atgaattaat agtatgtaga atagagaatg 59521 ctttcaattc attcaatctt agtctaataa tcgagaattc tttgcataac tagaaataaa 59581 tatgataaga agagatccct cggctgaacc gaattattct tcttagacag aatataaaaa 59641 aggaatctca aacatttaag taattattcg gagttaaact tggcgagaat agtactcgac 59701 aactaatagt tcatttatat ctaaatcaat ggattctcga ttcgcaattc cattcactaa 59761 tccttttggt ctattattct ctgaaaaaga gaacgttaga tgatccggta ttctcttttc 59821 ctggaagaaa tcattcgtat cttgttgttg tgatctataa gaagggcttg atccactctt 59881 tagactaatt atatctttgg gcttacaacg atagcttggt atatctatga tacaattgtt 59941 taccaagatg tgccgatgat tgactaattg tctagcagct ggaatagtag gagccatact 60001 caactgaaac acaatattat ccaaccgcat ctcaagtaat tgtaataata cctctccggt 60061 tgagccctta gctcttctag caatacgaac atatttaagt aattgacgtt ctgttgatcc 60121 ataattgaaa cgtaatttct gtttcgcttc caaacgaacg cagaattgag agacttttct 60181 tgaagcggat tattcaatag caattcgtgc ttctctcaga gtgtattgtt tattagtaag 60241 gcctggtaga tctttcaaac gacgtactag tttataacgg ggtcctctat aacgagacat 60301 agaaactcct tttctaacat tagagtagtc ttaaagcttg gaatagaata gggttctaaa 60361 taatctgaat gagccgttta cctgtttaca tttatgaatg atatgaaaca attattagtt 60421 tatagaacac tagtagttaa aataatcata acatagaaat caatacgagc aaataatcga 60481 ataattagta atgccttgaa aactataatc taagtacgta gaaagagtga aaagaagagt 60541 tagtaaatga acggatatcc attcatttca tatcgaattc gaacgaataa cctttgggtt 60601 gtgaattggt acgcagtttg atcaatattt tgtattctag ctattgaatt tttctcaaga 60661 aatgggatac cgatgttgaa tagttcaaag aatgaataat accttctttc ttattgtttg 60721 tagaaataga atgatataaa taaatatgaa taaattatct acctatagag atcgcccatg 60781 tatctaataa gtccacctct tgttgattgt attgattcat tgagcatatt catgtataat 60841 gctttatatg aattataaat aatatactag attctatcta aacttctagg cttagaaatc 60901 gaagcaaaga agagagatta agaaatctcc tgttggtatt tcaacaagat caaaaaagat 60961 caagacattt agtttatagg tattattcgg ttaggatagg taggtaagaa tccacaaatt 61021 ggattgcata tcaaagaaat ctaatcacag tcaatgtttc attattcagt aatatggcca 61081 tattcttggt ctgaggatcg ggagggggat atggcgaaat ggtagacgct gcggactcaa 61141 tcaaattgag cctaggtatg gagatatgcc aagtgatagc tttcaaattc aggggaaccc 61201 aggattattt ggtaggcaat cctgagccaa atctcgttta agagttacaa acccaatatt 61261 tggataacgg gataggtgca gagactcaat ggaagctatt ccaatgaata atcaataaga 61321 ttattttctt tgcttatttc tcgagtaaat agataatcta ttattacgta cgcatgattg 61381 aatcagaaat gataccctga agaataagga ttggtgaggt tgacaaagca agatcttctt 61441 tatgattttt gaatctcaga ttcttttttt tccttttttt ttagatctgt tctgtttgta 61501 ctcaaataga tattcctgaa gagataatag taaattaata atttaaatag ttttcacgaa 61561 tagagataga gtccagtttt acatgcctaa taagagcaac aatgaaaatt gtagtagaaa 61621 gaaaatccgc tggtctttgt agcaccgtga gggttcgagt ccctctatcc ccaacagaag 61681 aatataaaat caatcttatt ttctaatatt tgaaatattc tggaagatct ctgttttggc 61741 tcgaaaaaga aataagagga ttctttccat atcctcatag aaaatatcat tcaatcaact 61801 ctacagaaca aataaagaat agtaaaatat ttatcctagg tagattatat ttttccttat 61861 ctctttaagg agattaatac tcttatagat caggaaaatg actaaaaaac cctttaaaaa 61921 tctttaagga tagtttgcag ctgaaacgaa cccattcaag gaatccaatt ggattccttg 61981 aatgggttcg tttcaggatt aagtaagata gaagattatt tgaacgcctt aaaatgaata 62041 aaaataaata aaatccaatc tatcctgaga tctttgtgta gtggagtcat tagccgggat 62101 agctcagtcg gtagagcaga ggattgaaaa tcctcgtgtc accagttcaa gtctggttcc 62161 tggcatacct tattcaatct actatttaga attacatatc ctatgaaaag aataggatga 62221 tatttaaaaa agattgaagg atagtaaaag gtttctgctt aattactcaa taatgcgatt 62281 gatcattgta taccaaagac atctaaaatc cattaggatt atttagaatc agataataat 62341 taatacagtt atcttgggaa tcctttctaa ttatcccctt acattgctta ataggcatct 62401 tgcaactcat aaaaatcggg tatgacacaa tctttgcgta aaggccatcc gatccggctc 62461 tcgggcatca aaatccgctt aagacctgga tgactatcgt aaatgattcc gaacatatca 62521 taggattctc gctcttgaaa atcggagctc ttccagaccc aaaagacaga cgggattctt 62581 gggttttttc ttgacacaaa tatctttata catatctctt caggttgatc tgcattatct 62641 tcgatcttgg taagatgata cacactagct aaataacccc caggtactac atcgtaagca 62701 cattgggaac gaagataatt gaaaccgtac acaaataatg caacagctag acaaggccaa 62761 tcttctgctt tcacctgtgg gatctcgaca ccttggtaat caaaccctaa aggcctatgt 62821 gctaattcat atctagttaa ccaagtagat aatctacctt gcatctggat attatgatca 62881 tttttcttat caatgatact catattgatg aacatctctg ctgttctttt tttcttaata 62941 tatttatttt cttatttctg atactgattc ttcgatattt cttaatctat tttcaaactt 63001 atcgaatctt ttcagaaaac tatttaacaa aggtcttgaa gactgattaa tcagttgttt 63061 attatactta tcgttatgaa taatagatag aaaggaaagc cggtgattta gactgaaata 63121 tttatttcga ggtcgataat aagtttgact tctataggtt tcctgagcga tcttcttacg 63181 aagtttcgtt acagcatcaa taatagcttc aggtttgggt ggacaaccag gcaaataaac 63241 gtccacggga atcaatttat ctactccacg aacagtacta taagaatctg tactaaacat 63301 gcctcccgta atggtacacg ctcccatagc aattacatat tttggatgag gcatctgctc 63361 atatagtctt actagagacg gagccatctt catgtttata gtcccagccg ttattatgag 63421 atcagcttgt ctagggctag atcgtggtac tagcccgtat cggtcaaaat cgaatcgtga 63481 accaattagc gatgcaaatt caataaaaca acagctagta ccataaagga gtggccacaa 63541 actagagagt ctcgaccaat tcgagaaatc tattaaatta gttaaaaaaa tcgaattcga 63601 gatgtctgga ttagggatga gagaatcaag agaattgatt attagatttt gattttcggt 63661 agagtctcta ttcttatctt ttgaatagtt agcaattaag accattctaa tgctcctttt 63721 cgccatgcat aaactaaacc aataattagt atgaatataa agatgagcac ttcaacaaac 63781 gcgaatgcac ccaattccct gaaactcata gcccatggat aaagggaaac cgtctccaca 63841 tcaaaaatag caaataccag agcaaacata taataacgga tctgaaattg gacccaggtt 63901 tcaccctttg gttctatacc tgattcataa cttgttaatc tttctggacc ttcataggta 63961 ggtgcgaaaa gcctggaaac tgaaaaagct agataaggaa caaggccaga gagtattaga 64021 aagatccaag aagaatcata tttaggaagc aaaaacattt atatactcct cttggtattt 64081 ctcttcttct tatgtcatta ttaattatta ggtataacac ttgtatgtcc attaggacag 64141 cagttctcgt ttttctagca ctatttgata gagaagaaag cgatagatcc cataaatctg 64201 ctgttgtgtt gcagactgga ttgaaatatc aatgattaat agactggttt attagctttt 64261 cttagacgaa attgacatca ttttttctat tatcacaagc aaaattgaat tgacatttcg 64321 aatgcattta aatctaaaag aatcttttag atcaacatac attcatagat tttgtatatc 64381 tttttttctt accttctatt ctcgacattg aatagattat tattctgttt ttttccaccc 64441 taacagaact agtatcagtc aaaaatagcg gatttatatc acgtctttac ggtcgtgata 64501 aagatataat atttttatat tagtataaaa tagggctata cggattcgaa ccgtagacat 64561 tctcggttat gcagatcggg atatttttag aatccatgaa ctgctttacg aacccaacat 64621 gcttctttcg tagcattggg ctcttctatc aacgagtcgt cgattggcta gcaataataa 64681 cgatttgctg atgcaccatc cttcattcca ttcagactgc taagatggtt ccgtgtgctc 64741 aaatatttcc ttagaaatag agcaatcccg aagtcttttt gaaaaggttc ttagtattga 64801 cataggtttg ccttttgtag acacaaatat aatcgatctt aacctaaata ctctctatcg 64861 tttcgaaagg agatacgatc ctctttacac ctcagagttc gtagaacgag gaagagattc 64921 gcatgaggtc ctcgtaaagc cctcatcatc tttagcattt aaagagattc tttatttacc 64981 atatcaactt atcaaaattg actctcttag ttgtcaaatt ctttgccatg ctattgttct 65041 ttgtctttat cgagtgaggc ataagtattt cacatccgat ccgttctgtg aatcggttta 65101 agatcgatga accttttgtg agaaacatcg gcgcacgtgt aaacgaggcg ctctaccact 65161 gagctatagc ccttaatgaa atgaatctac gcttagaatc tataccaata tcatatattt 65221 agtatatata tatgtattga ataaagtcaa gttcgaatga aagaaatcaa aaggatcaaa 65281 ttacaataat gattgaaatc caatatatag acctattata attacaattg attctaattc 65341 tttcattgag tgaaagaaag tgtttcataa accacctgct taactcagtg gttagagtat 65401 tgctttcata cggcaagagt cattggttcg aatccaatag taggtaaaaa cttattggat 65461 gccatataaa tggatatggc atccaataag ttttggtttg aactggataa gtagggagaa 65521 agactcatag gactatcaag ttcttgtaca ttctatgata agagataaga atctattctt 65581 ttattatttt accaaataaa gattagaaag aattaggtaa tatttaaggc ttctaatcgc 65641 gctttagctc ttttaaatgc taaattcgct tcaatgatct tctttttccc ttcggctcga 65701 gtcaagttct ctttcgttgt ctgaaaagtt ctttgggctt cttcaggatc aatttcgcta 65761 gctctttctg cttcgttaac taatattgtt acttgattgt tatctaccat cgcgaagcca 65821 cccattaatg ccattgtgga ccattgccca tcataatgta ctttgagaac tcccatatcc 65881 aaagctgtga gaagcggcgc atgattgggc aatatgccca cctgcccact gttagtcgat 65941 agaataattt cttgaacttc agaattccaa acaattcgat tgggagccat tacacgaaga 66001 ttcaacatca tatctcttat tgaccctcca cttggagaga agcagccttt gtagtagctt 66061 cgtcaatagt accaactaaa taaaaagctt gttctgggag attatccaac tctccagaaa 66121 ggattatttg aaatcccttg attgtctcta ttagactaac atattttcct ggagaaccag 66181 taaagacctc tgctacgaag aagggttgtg acaagaaacg ttctatcttt cgagctcgag 66241 ctactgttaa acgatcttct tccgataatt catccaatcc gagaatagct ataatatctt 66301 ggagttcttt ataacgttgt aaagtttgtt taactccttg ggcagtctcg taatgctctt 66361 caccaacaat ccagggttgt aacatagttg aggtcgaatc caaaggatct acggctggat 66421 aaataccctt ggctgctaat cctcttgata gcacagtagt agcatctaag tgtgcaaatg 66481 ttgtagcagg agctgggtca gtcaaatcat ctgcaggtac ataaacagct tgaatagaag 66541 tgattgagcc ttctttggta gaagttattc tttcttgtag tgttcccatt tctgtaccaa 66601 gagttggttg ataacctaca gcggaaggca ttctacccaa taaagcagaa acttctgatc 66661 cagcttgaac gaaacggaag atattatcaa tgaagagaag tacatcttgc ttgttgacat 66721 ctcggaaata ttcagccatg gttagagcgg ttaaaccgac tctcatacga gcgcctggtg 66781 gttcattcat ctgaccgtag actagagcaa cttttgattc tgatatattt tgttcattaa 66841 taacttttga ttctttcatt tccatataaa gatcattacc ttcacgagta cgttccccta 66901 ccccaccaaa tacagataca cctccatggg ctttagcaat attattaatc aattccataa 66961 tgagaactgt ctttcctacc cccgcccctc cgaatagtcc aatctttccc cctcgacgat 67021 atggagctaa aagatcgaca accttaattc ctgtctcaaa gatagataat ttagtatcta 67081 actgtgtaaa tgcaggagca gatctgtgaa tgggaaatgt tgtactcgta tctacagggc 67141 ctagattatc aactgggtca ccaaggacat tgaagattca accaagagta atttcaccaa 67201 caggaacact gaggggagct cctgtgtcga caacattcat acctctcatt aaaccgtctg 67261 tagcactcat cgctacggct ctgacttcat tattacctaa taattgttgt acttcacaag 67321 taacattgat ctcttgacct gctggattct gtcctttaat taataaggaa ttatagatat 67381 tgggcatttt gcccggtgaa aacgctacat ctaatacggg gccaatgatc tgggtaatct 67441 gtcccacatt tttttccacc aatttagaaa ttccgaacgc gagaaaactg gttttcataa 67501 ctcttttggt gtattatctt tatttgatta ttaaatcgat ggaaatatag aatattttat 67561 tgctaatccg tccatggtaa agagttactt gtttcatact ctctccattg cccaagtgtt 67621 tcatttccct tctttgttac aaatagattc taatctgcat tagaaagatt aagaaagata 67681 ccatataaca agaatgattc tttcgtctag tacggtgttc gaggtcgcaa aaaactctat 67741 tctagtctgt ctttatgaaa gaaaacaaat ccagatgttg gtgtcaattc ctttattcct 67801 tctacggagc agtctaacca tgaaaagatt ccgcagtcaa tgtattgaaa tatcttaaga 67861 tttggatcga ataaatcgaa taactctcat aagagataag gagggttggt tgcattacat 67921 aggaaacaca ttatagaata ataatgttcc atacttggaa tggagtttcg atttgacaag 67981 tatttgtagt tcaacgattt aacaagaccc atttcacgct tcctattcat ctacatttat 68041 gtcgagcaga tcccatcttt gcaagattta ccaattgagt tataggaagg agggacctat 68101 gtcaccacaa accgagacta aaacaggtat tggattcaaa gctggtgtta aagattatcg 68161 attaaattat tatactcctg actatgaaac caaggacact gatatcttag cagccttccg 68221 aatgactcca caacctggag taccaccaga agaagctgga gctgcagtag ctgcggaatc 68281 ttcaacgggt acatggacca cggtatggac agatgggctt actagccttg atcgttacaa 68341 gggtcgatgc tatgatatcg aacccgttgc tggagaagag catcaattta ttgcatatgt 68401 agcctatcct ttagatctct ttgaagaagg ttctgttact aatctattca cttcgattgt 68461 aggtaacgta ttcggattta aagccttacg cgccctacgg ttggaagatc taagaatacc 68521 tcctgcttat tctaagactt ttatgggccc acctcatggt atccaagttg aaagggataa 68581 actgaacaag tatggtcgtc cttttttagg atgtacaatc aaaccaaagt tgggtttatc 68641 tgctaagaat tatggtaggg ctgtttatga atgtcttcgt ggtggactcg attttactaa 68701 ggatgatgaa aacgtaaact ctcaaccatt catgcgttgg cgggatcgtt tcttgttcgt 68761 agcagaagct ctctttaaag ctcaatccga aacgggtgag attaaagggc attatctaaa 68821 tgctactgca ggtacatgtg aagaaatgtt caagagagcg atctttgcta gagaattggg 68881 agcacctatt gtcatgcatg attaccttac aggaggtttt actgcaaata ctagcctagc 68941 ttactactgt cgagataacg gcttactact tcatattcac cgcgcaatgc atgctgttat 69001 tgatagacag aaaaatcatg gtatgcactt ccgtgtatta gccaaagcgt tacgtatgtc 69061 tggtggagat catgtccatt ccgggactgt agtaggtaaa ctggaaggag aacgagacat 69121 tactttagga ttcgtcgatt tgctacgtga cgattatatc gagaaagacc gaagccgtgg 69181 tatctatttc actcaagatt gggtctctat gccaggtgta ttacctgtag cttcaggagg 69241 tattcatgtt tggcatatgc ctgctctaac tgaaatattt ggagacgatt ctgtcttaca 69301 gttcggtggt ggaaccttag ggcatccttg gggaaatgca ccgggtgcgg tggctaatag 69361 agttgcatca gaagcttgcg tacaagctcg taatgaaggg cgtgatcttg ctcgcgaagg 69421 taatgctatt attcgtgacg ctagtaagtg gagtcctgaa ttggcggccg cttgtgaggt 69481 atggaaagag atcaaattcg aatttgaagc agttgataaa cttgataaaa tcccctaaat 69541 attaagaaat acgatatatt gattatagtc gaggccgggg agtattgaga taatgcttgt 69601 tttatccata ctcctcataa cgatttgatc aattgagaat tggtagctca gcggataaga 69661 gcacatggtt ccgaaccatg gagccggggg ttcgaatccc tcccgattca caaacgaaga 69721 cagaagctgc atagaatgaa agagtaaaga atctaagatg caaataagat ttataaatta 69781 aatccaattg aaaaatcatt ggaaaatcaa ttgtcaggtt ctaacatgtg attgatcaaa 69841 tggataatca tttgattgct aatttgttat tggatccgga gttagtctca agttgatacc 69901 cattccaact taataatctg ctgttccaga aatgatttct tcgtttctgt attatatgaa 69961 tgagatctaa tgagcttgat tgttcctctc ttaaaagggg aagaaataga tgaggatatt 70021 cttatgtctg taagaaatcg gtttgaagat aaacgcaaat tgggtggatt gatcgaagcc 70081 ttccttgaga aagctaccaa aggttatgtt tcgagtgaaa gagagaaaga tagacatata 70141 gcgatcgata ttaataaagg attatggaca cgctgcgata attgtgggaa catgctctat 70201 gtaagatttc tgaaacagaa taagagtgtt tgcgaagagt gcggctatca tcttccaatg 70261 actagtacag aaagaatcga gctattaatc gatcgtgata cttggactcc gatggatgaa 70321 gatatgactg cataagatgt tctaagtttc tccgatgagg attcttatca aaatcgtatc 70381 attacttccc agaaaagaac aggtttaaca gatgctgttc aaacaggtat aggatatcta 70441 aatggaaccc ctatagcgct tggtgttatg gacttccaat tcatgggagg tagcatgggt 70501 tctgtagtag gtgaaaagat tactcgctta attgaacatg ccacagataa gtctttgcca 70561 ataatcattg tttgtgcttc tggaggagcc cgtatgcaag aagggacttt aagcttaatg 70621 caaatggcta agatctcgtc tgttctacaa atccatcaag tccagagaag cttattatat 70681 atatcagttc ttacctatcc aactacaggt ggtgttactg cgagttttgg tatgttaggg 70741 gacattatta tcgctgaatc taaagcatac atagcatttg cgggaaaaag ggtaattgaa 70801 taaacgttac gtcaaaagat accggatggg tttcaagtag ctgaatcttt atttgatcat 70861 ggcttactcg attcaattgt tccacggaac cttttaaaag gtgttttgag cgagatattc 70921 gagctttatc cttcagttct tagcaaacca aattaggttc tcttcgttct ttgatgcagt 70981 taagtaatca gagattgttt ctaccattct tctagataac ttggaaaaga gttaaggttt 71041 catgattgat tcctttttta ccgatttttt tcctgtttct ttgaagaatg actatttttt 71101 tatcaaccaa aaatcaagat attcttttgg ttctctacga tacaatacta tgagaaaacg 71161 aatgttggta taatacaatt gacaattctg agtagtggat cacagttatc tattactata 71221 attccaggtt agatctcacc cttagaattt tcttgataca gatatattct tttctttaca 71281 ttatttctag agtaatctgg agttgattga agatggaata aaagaatgaa agaaaaaaat 71341 agaggtatta tatccatttc tgagatggac ttcttaatag ataaattggg attagaaaga 71401 cttcttcttt ttattgaaca aaaaggatat gatgaaatat tggagaacag agagaaatca 71461 tatatatctt ctttatttga gggaattttc ttatgacagc gtcttatcta ccttctattt 71521 ttgtaccttt agtaggttca gtctttccag ccattactat ggcatctttg tttatttata 71581 tagagaggga tgagattctc taaatctaaa ttcttatttt ctatgttcct cttctggaaa 71641 gaatgtttca tagaatagat taaataataa tatggattcc tattctaggt tatttggtta 71701 ttagttagta ttactcattc atttgaaata aaagcttgac aatctttgat tgtatttcaa 71761 tctatgtttc catttgaaca agcgaatgaa acatagattg ctgttccatt tgcaaaatat 71821 atagatcgaa tctccgtaga agaaatctat atctctctat aaatatccat tcttatataa 71881 cagagcaatg actaataata tcaatagtct cgattctttc agattcagga tctattcttt 71941 gttagataat atctaaaagt ctcatgtatt acaaaaagat gcaaaacaga cagaatcgat 72001 aacatttcat ataactgctt gattagaaaa gaaagaagaa atctttaggc ttttggggag 72061 tagtaatgac aagttggcaa tctaaatggc tccgaataga ttatataaaa ggttctcgta 72121 gattcagtaa tctatgctgg tcttgtatcc tcttttgcgg tgcaatgggt ttctttttgg 72181 ttggtctttc tagttatatt ggtaaagatt taatccccat actgtcttct gaacagatta 72241 ttttcattcc acaagggatt gtaatgtgct tttatggtat tgcaggtctt ttcttcggat 72301 tatatctgtg gtgcactata ttttgggatg ttggtagtgg ctacaatcta tttgataaaa 72361 aagaagggat cttttctatc tttcggtggg ggtttccggg gaggaacagg cgcatttgta 72421 ttcgatttgt gatgaaggat atataagcag ttagattgga aatccgagaa ggtttttatc 72481 cacgtcgaat tctttctttg aagatgagga atcgaaaaga tgttcctcta actcgtattg 72541 gtgaagattc gacactgaaa gagatagaag aaaaggctgc cgaacttgct cgtttcttac 72601 gtgtatcaat cgaaggattt caagattgaa taaatattct attcttgctt aaagataatt 72661 aagaatagga gataggatag aaggagaata gattgatatg catataataa agataaaaga 72721 aatccttcaa attcataaag atcattcagt tgtaacgagt tatctaatag cgaatgcttt 72781 ttgttagaac aattattgct ccgtaattct gtatatatat aatatatgag atcatattgg 72841 tggaaacttc tccagtggtt ttacaacact ccatatcgtt cgttaagtag agcttacaaa 72901 gcaagtaagc agatacaatc tattggaaaa gactctttgg cctatataga aagaagatca 72961 tctttacaat acttatcgca agttgtaatg tctcatatga atatggaact tgataaatcc 73021 atatttctga tatattggag tctcttagaa tgtaggatca gtctatccat accacagatt 73081 cggaatagat ggaaacttct ttcttctaaa ggaaaccatt ttgactcgct cttaattgag 73141 gattattcct cttatcccta taaacgacaa tcatcttcca aaaaagaatc tgatcttgta 73201 tcttcgtttt tctgttcaaa ttcctctaat cgtgatagaa gacaaggcaa tagaaacagt 73261 ctattatata atcaatcaga agatgctctt cctacgacat taaaaagctg tatatcgaga 73321 agatcagata agataaaaga aatgaataag agattaactt ggattgaagc aattttgaat 73381 gatcttgatt tctggagatg gcaatgtttt acaagctctg catcttctca aatgaataaa 73441 gagtcagata aggaattgtt gattcgggaa tcaataagct tttcaactaa tataacagct 73501 tacgaatcta tcagtctaat accacgttct ataactcgta ccctatccag attcaggaca 73561 gagttagtga atcaatcagg cttgttgtta ttcaccaaga ttgaattggc taaatatcaa 73621 gcattagctt ctattcaata tattggatgt ttattattct ttccatctat aataccaatc 73681 attctaaatc attggtttct agaaccttgg ataagggact ggtggaatac ttctcaatca 73741 cagatattta tcaatctatt tcaagaagaa aaagctttga agcggcttca agaagtagaa 73801 ggattagcct ggttaaatga aatgctcaca gattctgtag atactcaatc ataaaattat 73861 gatgcaatcg tatacgacaa aactattcaa tcagtaataa tttataatga aggaaatatt 73921 caagcaatct cacgctttgt aaccgatgta attagcattg ctatttcaac ctctttgttt 73981 ataataggtc gaaaaagact tgcagttcta aattcttgga ttcaagagtt attctacagt 74041 ttaaatgata caatgaaagc attctctatt ctcctgttaa ctgatttatg tattggcttt 74101 cattcccccc atggttggga aatactaata ggttccttct tagaacattt aggatttgct 74161 cataataaat atgtaatatc ttgcttcgta tcaaccttcc cggtcatttc agatacagtc 74221 tctaagtatt ggatctttcg ccacttgaat cggctatctc cttcaattgt agcaacttat 74281 catacaatga atgagtgact attctctttc ttcaagcaat tgtaatatga tttcttctcg 74341 taagtaaaag atctttttct ttgatattga gaaaaaggaa ggaacccttg ataattcccg 74401 taagaatcaa actctgagga taatatagtc ggaataacga acgaaatctt gagactctat 74461 gatgtggaga aacaatatgt ttccaacaaa attcgacacc aataatcaaa ctatgcaaaa 74521 aaaaaatatt aataattggc tgaataaatg gttgattcta tcgttcttaa tatcggtcat 74581 ttttaccaaa ataaattgtc catctgtttc aagagcatac cctatctttg cgcaacagaa 74641 ttatgagaat ccacgagaag caactggacg tatcgtatgc gctaattgtc atttagccaa 74701 aaaagctgtt gatattgaag ttccacaatc tgttcttcca aatactgtgt ttgaggcagt 74761 tgttaagatt ccttatgata tgcagataaa ataagtactt gcaaatggta agaggggcgg 74821 attaaatgtg ggtgccgttc tgatcttacc cgaagggttt gagcttgccc cccctgatcg 74881 tattccaact gaaaccaaag agaagctaaa taaactatca ttccagaatt atcgtcctga 74941 gactaagaac ataatagtaa taggtccaat tccggggaag cgatatagtg aaattgtatt 75001 tcccattctt tcaccggatc cttctactga taaagaagca catttcttga aataccccat 75061 ttatgtaggg gcaaatcggg gaaggggaca gatctatcct gatggaagta agagcaataa 75121 tactgtatat aatgcttcaa taacaggaag aataaccaag ataatacgta aagaaaagaa 75181 gggtggatat gaaatcacta tcaaagaagc atcagagggt cgtgaagtga ttgatattat 75241 acctccaggt cccgaactta ttgtttcaga gggtgaatct atcaaagttg atcaaccctt 75301 aacaaataac cccaatgttg gaggatttgg tcaggaagag gcagagattg tacttcaaga 75361 tccattacgc attcaaggtc ttttactttt ctttgcatca gttgttttag cacaaatatt 75421 cttggtactg aaaaagaaac aatttgaaaa agttcaactg gcagagatga atttctaaac 75481 caatacttta taaatgcaag atattttgac tgaattgata gtcgtttctt ttcgtactga 75541 ttgtaaatcg aatcgatcag ggtttcattt ccaggtatta aaaaaagaat cgaactcaat 75601 tggttatttt atcagtaaga aaagaagaga tcctttgata atgtctatag tctccgttta 75661 agtcagatac tagatatttg aagagaattc ttgaagaatt attcaaacaa taaacttgat 75721 aactaccttc taattctggt agataaacta tctttgttag gagattgtct cttaatcctt 75781 gatctggttc ttatctcttt caatttgatg agtgtagtaa gatagattta ttgatcgatt 75841 gcagcttcgt tactctttga atggaaagga aaagcacaaa tcagttatta gaaaagaggg 75901 ataagaaacc aatgagaaac attgagttgt cttaccttat attaaattgt tggtcctata 75961 ggatattcaa atttcaagta acagccctat ctattctgcg gaaactcaga ataaaagatc 76021 tttgaactct taattggatt cattggatct gtttcacaaa gcatatagtt ctctttctag 76081 attcgaaaga gaatatgctc aactcttatc aaatgattgt aataattttc attatactat 76141 tctgatcgat tctcttctaa agaatcgatc aaatgtttta gttatctgaa taaagtaacc 76201 tgatgctttt ggaatcaagg aacattgata atcaaaacca aggtatttcc ctcctcagat 76261 tgatatacta tatttcattt ccaatcattt tctcttctta atcttcaaag gtaatagtaa 76321 atataaagtc ttactcaata ggttctctga atcataagta agagaaaata tccgatgctt 76381 ttttattaca tcatagagat gatcctaatc ctgaataggc tccgtaaaag aatatcccta 76441 ataaacctaa cacaagtata ccgattacag tacctattag ccacagagga atccttccag 76501 tggtattggc cattcttccc actccctttt cttcttttgt ttcattattt tcttttgtca 76561 cgactcgaga catgaacagt cataaatgat tggaatccca gttcttttag atcaagatta 76621 acagatcact ctctaattga agaagtaatt agaaaataaa acggcaagca caaagatcaa 76681 taagagtccc cagtaaagac tcgtacgatt caattctaca ctctgtttat tcgggttcgg 76741 tggtgtcatg gctgtgcagt ttctgtaaag actatcgttg aataaattgc attgctgata 76801 tggatccgag aaagaagacc gtgggtacag ctaatccgtg aacagccagc cacctaacag 76861 tgaaaattgg ataagttcta tctagagtca ttagtacctc ctaaaaggat ctagtaaatg 76921 catcaacttg ttccaacgaa tcaaaacggc cggttattaa aggaacttct tgtcggctct 76981 ctgtaaaata ttcatttgga cgagggcttc caaatacatc ataagccaaa cctgtactga 77041 caaataacca ccctgcaatg aagagagaag gtatagtaat gctatggatg acccagtatc 77101 gaatactagt aataatgtca gcaaaaggac gttctcccgt gttcccagac atgtataact 77161 ccgtatatct tttatagtct taaggaggat tgtttccgac gaggatgaat actaggagat 77221 atagaatcta ctagtacacc tattgatatc aatcctttaa tagttaggtt gtcattacat 77281 accaatggta tatcagaaat atactaggtt agtatagcta gtctcccttc taagcagcaa 77341 gattactttt ttttaagaaa tatataatta gaaagattgt attcctcttt ttcttaatcc 77401 ctttctattc ttgcagaata ccgccactct ttatgctcat aatttcaatt tcttatcctg 77461 agattctcaa aaagtaaata atcaaacatc attgaccttg caagaatatt tgattctaaa 77521 gataaagtag gaatattttg atcaatcatt aggctatgct gcttattttc aagaattgaa 77581 aacattcaat ctacaaaata tcagatagtc catcggatct ttgaaaaatt gttttttaat 77641 ctttaacttc ttcaattgta tatcgagcca ctccgaattg attgtagtat tctatattgt 77701 cctttctttt ctttaatgat taatcatcga gagatctaag aaagaaaaga atgattaaga 77761 atcatataag gactaagaag actaatccaa gtattgatca ttaatcttaa tcttttgcct 77821 ttattaaata gaaagaaggt tcttttcaac ggatatttta gaacaagaat tagtaatcct 77881 taatcaagat tctaggtttg aatctgaatc ctattggata ataagttagt attatcaact 77941 taataatcct ttaatcatct ttatttagat gagtgataag taataaacta tttcagaaaa 78001 ttgtatacta atactggtgt atttcttagt ttcatctgat attcaaatct atgcttactc 78061 tattaaatta ttttgctttt ttagcatctc tgctaatttt aactttaatt ttatttgttg 78121 gattgaacaa gattcgactt ttatgatata gaaatattat ctagaaaagt ttgacttgag 78181 gaggaccttg attatgttgg tgcttactta tttactgcac ttatcgatca tttattatta 78241 ctattcagat cgctttcgat aagtaattct aagtctatta tacatttcaa aaataaaatg 78301 gttgaagcat tactatctgg aatcgtttta ggtttaatcc caataacatt agccggactc 78361 tttgtaactg catatctaca atatcgacgc ggtgatcaat tagacattcg atagcaatag 78421 aaaagcattc ttaacatatg tcaacaatac ggagaattca aagagtatca aactattccc 78481 taaaaaaaaa agacatagaa ttgaatgaat caatctctag aagaaccacg ccctgtagga 78541 tttgaaccta cgacatcagg ttttggagac ctgcgttcta ccaaactgaa ctaagagcgc 78601 tctctttcta ttttatcaca atcagttgtt cttctgatta gaatctgtcg atattttgat 78661 acgtgggaat aattaacccg accggacaaa tatttggctt cgttttgatt tttaggagtt 78721 atatcataaa cataagaact atcaaatcaa atagggatga caggatttga acctgcgaca 78781 ttttgtaccc aaaacaaatg cgctaccaaa ctgcgctaca tccctcttgt cgttgatgaa 78841 tctttaatta aattagcatt ctggatccat aaatattcca ggaaggccga gtacgtacca 78901 gatgattata acgaataatc ataaggattg gctactcgct ccagatctaa ttccatcatc 78961 taactacatt ccaagaatgt attatctctt gtaaatctga aagatttaat tcctctttta 79021 tagatgattg tgttagaaat attaataaaa agagacataa tctttcatct atttgattaa 79081 aaagaataag aaataaggag aatttagaat gcaagacgtg aaaagttacc tttccacagc 79141 ccccgtacta gctacactat ggtttgggct gctagctggt ttattaatag aaatcaatcg 79201 tttctttcca gacgctttga tattcccatt tctttaatcg ctagaacgag agaataaaga 79261 tcaaacaaaa tagcctagaa aaaaaagaga agtaaaggga gagggtttaa tgatccattt 79321 ctattgatca atagaaatga cttataagct tgattagtat ttgatcgatc tattatcaag 79381 acataatttt tatagctcta ttcctcaatt tcttatttct ttccattaat tctatgacaa 79441 agactaaaga catcagaata acgatatgtt tagaatgtac agagtgtgtc caaagagaga 79501 ctgatgagag atctcgaggg atttctcgat ataccacaag aaagaaccgt cgcaatacac 79561 ctacttgatt ggaattaaag aagtatcgtc cttattgtta taaaaatact attcacaaag 79621 aactgaaaaa atgaagcatt tctttctatt aagtgagaaa agagtcagta attctgatag 79681 ttctgagaaa gattataaga gaatattatg aatcaaggta atcgatcctc tcgtcgacgt 79741 tctccatcta tttgatctga tgaaatcatt gattatagga atataagttt acttcgtcga 79801 ttcgtaggtg aacaaggaag gattttgtct agatgtgtaa ataggttggc cgcgaagcaa 79861 caacgctcaa tagctatggc aatcaaaagg gctcgtgttt tagctttgct acccttcttg 79921 aataatgata attaggtcat ttcttaatta aaaccagaag aatgaattag aataaagaaa 79981 aagatttaat attctacagg ataagataaa ccaactaatt atctaatcaa taaatagaag 80041 agaatcatta ataggataat cgggtgaaaa ttactattca tagtacaaga tccaaatcca 80101 ccaagtacca ggcctctcca aatatgatgg agaggctttt tatttgtcca taagtagagt 80161 catatactaa tcatttgtac gattctggaa aaacaagaag tgtctgaaat agctatctat 80221 gcaagtgttt tacgatttga ataaacttga tttctataca aatgattgat catattatta 80281 taatttgtca cattctctcg tgttgctgca ttaattcgcg caatccataa acgacgaaat 80341 tctctctttc tattctttct ttccagataa gcggaagctg atactttcat ttgttgttgg 80401 tttgctgttc taaataatct cgaatgagct cccctaaatc cagatgtgat tctaagaata 80461 gttttgcggc gtttccgagc cacagatccg cgtttcactc tagtcattag atgtctacct 80521 tcatagaaaa taaaatctta gtttgttaag aatctatttg attgatactg aatgaatcta 80581 tggattagta ttacaagata tttatgttcc ttattcttct tgaaaattta aaagaaattc 80641 aataagaaac aaaatgaata ataagcccaa gtttttaatt attaggtctc tcttatattc 80701 aattccgatc gcattcattg gaagtatttg ggatagaatc tagatatggt tatttaataa 80761 gttgagatta atagattctt tgaaagactc ttgagataac ttttttctct attgtaaaag 80821 atatagattc taatggcttt tgctactcta accttcctat ccgcatattt gtaaatataa 80881 aaattaggca tctatttctt ttaatactaa tgtattatcg tagaaaagta cggattccaa 80941 agtttccata gtatctttct taacagttag gtccttccta tataccagag cttctaattt 81001 tcctatatca aagaaaatgg acttaatgct ggtaagctgt atagcttcca atctatctat 81061 ctctacttta atgaaatcac taagtaaatt ttctaaacaa aagtctagta acgatatgtt 81121 attttcggaa gttgtctcct caaatagctg acctgaaaaa gctgtcattc aaaagggtct 81181 tagaaaaaaa tctaaagatt tagaccacta tttttatttc gcttaatgat ttttttacta 81241 tcattgatat ataatcattc atctggcacc aagataattg gatttgcacc aatcaagact 81301 atggattttc atccctcatt agaagaggtc gatgataaat ctttctcttc gttcgataaa 81361 ataaaggatt tgtctgcaaa atcaatcctc tcatatcgtt cgaattcaga ttctaacaag 81421 tcgcacatac accctagtac atactcccct ccgttggggg catcctcgaa gggctgggga 81481 ttttgttcca ctctccatgc gttgtctagc tttcctaata agttgttgat tggttggcat 81541 gcgtgaaaat ttgcagaaat ctttggttca gaatattcgt aaccattgga aagtatccat 81601 gaattagcta taagccgttt ttttataaga tttgaatgat aatgattctg tcttttataa 81661 ggaagtcata atataatcaa ttatttgatt ggagaaagag caaaggtttc tattaataga 81721 ctttcgtttc ttccatgaat caataatata tcgatgaccc caagataatg gagtgtaggt 81781 atcaataaag aataaacaat agttttattc ttaaatactt tcattcttga ttgcaagtta 81841 ctgaacggat cttgaatctg agtagttttc tgatgctaca aggtctacaa taccataatt 81901 cttggcttct tttgctgaca tgaaaacatc tctctccatg tcttcagaga ttacccatga 81961 ggctttgccg gttctttgta cataaaccct agtaatacaa tcacgaagtt tcaatacttc 82021 ttctgcttcc atgatgcatt cccctgcttg tccgtcatag tacgaactag caggttggtg 82081 aatcataacc ctagtttaat aatctcatca tttgatttgg ggtaaccgta caggcaattt 82141 tctctgcata cggctctata tctgtatttg atgaaagagg agatgataaa tgcttttagt 82201 cgtatcagcc aaataaactt cttatctatc acccacaact aacttatctt tgatacacca 82261 tctttccttt ttaccaagta ctatgatggt tctgttgctt ctttgtttct agcaatgcca 82321 aagttttcta tgaatattct agaataggtc tccattttgg agaaaggata tcagctttta 82381 cttcgtatgt aatatagatc ttatgacgct agaatagaaa tagtgaatgg tgcttaataa 82441 ttcaagaatc aaacaatcct tttcatcttt ttatttctca ttatcttggt gtttgtctat 82501 tttatttatc cagaatgaac caactttgtc ttttatgcca tgctattatt accttaattg 82561 aattgggcga aggcatagtt tcaattcata caaaccattg gcgccaagcg tgaggtagtg 82621 ctatacgttt ggtaatttct ccgccagtta gaataaaaga tcccattgat gcggctaatc 82681 ccatacaaat tgtatgtaca tctggtacca cgaattgcat agcatcataa acagatattc 82741 ctggtaatac tgctccgcca ggagagttta tatataaaaa catatctttg gtctcatctt 82801 ccccatttaa gtacatcata ataccaataa gttgatttgc aatctcatca tccacctgtt 82861 gacctaagaa aaggagtctc tctcgataaa gccggttgat taggataggt ctcttctgtg 82921 ttcccccctc taaaaccgta caagcgctgt taaaagcata cggctccaat agttatagga 82981 taagaaaatt gtacatcctt aaaaaagaag tttctttttt tttagagata aaggaatact 83041 ctattgtttg aaacccttac tatcctgttt ctgattcaag tttgtctttc aattctttca 83101 aacatcctga attgtatctt gattagtata atgaaagata ttttcttaca tcttattcct 83161 atactaatct tttcttgttc gtaatcaagt cttttagaga gacaacaaga agaatcttta 83221 ggctcatttt ccacttcgag gaggtatcaa tccgatcaat atttgtacat atggaacaaa 83281 ttgttttact ttttatctcc ctatttcttt ctaatttaaa gactcttcaa agtcactttc 83341 tatcttgtag ttaaccttgt tattatttct tattgggact tagtgatcga agcctaacta 83401 atctgttagt ttcaactagc catggattct gttaattaat agatctttag caaaagatct 83461 cattctaatc tcacgcgttt aacaattgaa acattggtcg attctaagtg agatagaaac 83521 agattgatcg gatcaaaaat gatgggaagt aattccatac aactcaatat atgcaagaac 83581 aagtcgctct atacgtcgac ccaaaccgcg tcctcttccc caggtagacg aaagggcacc 83641 ttcggaacac caattggcat gcagaaatag ataaagagat ttatttctct ctggattgaa 83701 aacgtaaaac aagaatagag atctctggga cattgtagca tatcgagtca attttgagtc 83761 tttcctgttt gaatagaaat gaaataatag aatatatgaa tgggaccaat ctctgcattg 83821 ttatataaaa aattcaaatg ctaaaatatc tttgtatttt tgtttcttga aaagaagatt 83881 tcaagctctt cccgttttac taatatgttt ctttaattta atattgtatt agaaacaaat 83941 agaaatagtc cttgaaaggg agtctcttat ctgattagga tcgaagagaa tttttattat 84001 ttccctatta ggtcttcaat gcaagaaatt tacataatat tcattctgaa taaaggggtt 84061 ttccatgggt ttgccttggt atcgcgttca cactgttgta ttaaatgatc cgggtcgttt 84121 aatttctgta catttaatgc atacggcttt ggtttctggt tgggctggtt caatggcttt 84181 gtacgaatta gcggtttttg atccgtctga ccccgttctt gatccgatgt ggagacaagg 84241 gatgtttgtc attccattca tgactcgtat tggtataacg aaatcatggg gtggctggag 84301 tattactgga gacaccgtaa cagatgcagg tatttggagt tacgagggag tagctgcggc 84361 tcatattata ctatctggtt tgttattctt agctgctatc tggcattggg tctattggga 84421 tctagatctg tttcgtgacg aacgtacagg gaaaccatct ttagatttac ctaagatatt 84481 tggaatccat ttatttctgt ccggagtcct ttgttttgct tttggggcat ttcatgtaac 84541 aggcttattt ggtcccggta tttgggtatc agacccttat ggattaaccg gaaaagtcca 84601 accagtagat ccggcttggg gagctgaagg gtttgatcct ttcgtcccag gagggatagc 84661 ttctcaccat attgcagcgg gtatcctggg tatattagca ggtttgtttc atctgagtgt 84721 tcggcctccc caaagattgt ataaggcttt gcgtatgggg aatgttgaaa ccgttctgtc 84781 tagcagtatt gcagctgttt tctttgctgc ttttgtagtt tctggaacta tgtggtacgg 84841 ttctgctgct actccaattg aattatttgg cccgactcgt tatcaatggg atcaaggata 84901 ttttcaacaa gagatagatc gacaaattcg tactagtaaa acggaaaatc ttagcccatc 84961 tgaagcttgg tctaaaatcc ctgaaaaatt agctttttat gactatattg ggaataatcc 85021 agctaagggg ggattgttta gagcaggtgc aatggacagt ggggatggaa tagctgttgg 85081 ttggttaggc cacgctgcgt tcaaagacaa agaaggtcat gaactctttg tacgtcgtat 85141 gcctactttt tttgaaacat ttcccgtagt gctggtagac ggagaaggta ttgtgagagc 85201 cgatgttccg ttcagacgag cagaatcaaa atatagtgtt gaacaagtag gtgtaactgt 85261 tgaattctat ggaggtgagt taaacggtat tagttttagt gatcccgcta cggttaagaa 85321 atatgctaga cgtgctcaat tgggcgagat ctttgaattt gatcgtgcca ctctaaagtc 85381 agacggtgta tttcgtagta gtcctcgggg ttggttcacc tttggtcatg ctacctttgc 85441 tcttattttc tattttgggc acatttggca cggtgctaga actctgttta gagacgtgtt 85501 tgctggcatc gaccccgatt tagacgctca agttgaattt ggagcattcc aaaagttagg 85561 ggatccaagt actaagagac aaatcgtttg aaagatcttt tatwtrcttg atttaattcc 85621 taaagtcata ggagtaaaat agtattattc acacaatcat tatctaatcg atatataccc 85681 agatggtcta acttgttagg ctattcagat ttaaaacaat aaaagatgag caagaaaaag 85741 aaagaaaaac ctattgaata gtgttgaata aacatgacca aaataaggaa aggaaccttc 85801 caattttaat tagtgcgaaa gctatcctta aaatccactc tattatcgaa tccatggaag 85861 cactggttta tacatttcta ttggtagcta ctctgggtat aatattcttt gccattttct 85921 tccgggaacc tccaaaagtc ccgactaaag gaaagaaatg atactttttt tcattaacca 85981 tggagaaaca aatgtttgtc taactagaaa tataaaaaga gaggagaact agttaaagac 86041 cttcctagaa gagaaaagga aggtcttctg gaccgattag tcttcatgct cttcaaaagg 86101 atctctaagc tctttcgagg gttgaccaaa agcagtatat agagcgtaac ctgtgaagct 86161 aacaagtgaa caagatataa atatagtgac caaaattgca gtttccatgg ttagatgatc 86221 caaagaataa tgattgtgtt tagtataata ctagtatata aagttagcaa ttcttaatca 86281 atcgaacaag ttatatggct acaaagatta ttgatgatac tcctaaaggc aaaccgaaga 86341 ttagtgtctt aggaatcttt ttgaaaccgt tgaattcgga atatggtaaa gttgctcccg 86401 gttggggaac cactcctcta atgggattct tcatagctct atttgctgtc tttttagtca 86461 ttattctaga aatatataac tcttccgttc tattggatgg tattcccata acttggtaag 86521 aacagaaaat aaaaagattt ggttgctatc cgaaaacccc cacagttata atagttgggg 86581 gatcacaatg aaagctaact cttctagaaa tgggtagtta aatcgtgtaa tttatttctt 86641 attcagaggt cttgacacta tgggtgtgtg actcgacata atcaatctag tgtgatagat 86701 agacgatcta ttatccatag gaaaagatat tatcatcttg ctagatagcg gtctgattat 86761 tagcatcgaa agcgcaagaa caaatctttg ttctgataaa gaatttgaac tatgatcaat 86821 ctatatgaat ctactatatc aaacttcgtt ccaagatcac tatattgatg ctgaattaat 86881 cttgagataa gattctattc ccaaattaat caaagaatta aagaaatcac ttatttctat 86941 atctcttaaa agattctgaa taatcttgat aatatcttag tagttctcat tttacaagat 87001 acaaatcgaa ccaaagatga atcgttaccc atttgattct cttacccaaa tattgaaagg 87061 attcgccgag gtatagtaga gatccaaggt tcatagatac ctagatagtg agattgaaac 87121 gagataattt ttattcaata tcgctctatt gataagataa aaagattttt caagacacca 87181 aaaataatta ttcctattag gaataagaga aaacatgaga ttgaagcaaa gcaggttctt 87241 ttttcatcga ttagagtcat atttcttttg tttctaatct taatgaatca agctaataga 87301 ttccattgaa agaaaagttt ttactaataa actaatcgaa gaatcgatgg ttccaatggc 87361 tttgtttaag ctgtatggaa tttgatctcc atgtacagtt tttgaagggg gagtcagaaa 87421 ggcttaccta tcttgataaa gtatatgatt ggtttgaaga gcgtctcgag attcaagcga 87481 ttgctgatga cattactagt aaatatgttc ctcctcatgt aaatatattc tattgtttag 87541 gaggaataac gttgaccagt ttcttggtcc aggtagccac tggttttgca atgactttct 87601 attatcgtcc aacagttaca gaagcttttt cttcagttca atatataatg actgatgtta 87661 attttggatg gctaatacga tccgtccatc gatggtcagc cagtatgatg gtcctaatga 87721 tgattttaca tgtattccgt atttatctta caggagggtt caaaaaacct cgtgagttga 87781 cttgggtaac gggtgtaatt ctagctgtat caactgtatc ttctggtgta actggatact 87841 ctttaccgtg ggatcagatt ggttattggg cagttaagat tgtaaccggt gtaccagaag 87901 ctatccctat aataggatct ccgttagtag agttattaag gggaagtgtt agtgtaagtc 87961 aatctacatt aactcgtttt tatagtttac atacctttgt attaccgctt cttactactg 88021 tttttatgtt aatgcatttc ttgatgattc gtaaacaggg tatctctggt cctttataat 88081 tatggaaatc catagtttta ataaaagaag attgaatcga gtcatctcaa aaccctaatc 88141 tatataaaat ataaagtaaa taaagattat ttttttattg ccactagtat tagaatacat 88201 tgaatcctaa aattattagt ggacttcttt ttatctcgat taagttgcca aaaagaagag 88261 attgaacaat ccttttttag aggagaatat tggattatgg gagtgtgtga cttgttataa 88321 aacagaggct ttgcagatat tccatcaagt actgccatat ccagagatat gtctcttctc 88381 caatcctctc taatcttcgg atcagagttc aaaacttgga tattaaatcc ctttgtatta 88441 tctgggggat cttagagaat tctttttatg attgaaccaa attaatcttt tggaaggctc 88501 cgttaaatgc caaatatatg tatatgctat taatatgaat ctaatattaa tacaatcaaa 88561 tgtcctttat attgaccaac atggttcttg gaacgcatcc atttcctttg tatccaatat 88621 gatggagaga gaccgtcgga gtgaaagaac gatctaaaaa gagataaagc caaagttcca 88681 acaggttaag atcctgtatg agactatgaa gaatcttata tctatagtta tgtacaatag 88741 atataaagat atatacagac agggtatgag atcatccaag aagtgtatca atcaattctt 88801 ttagctgact tggataataa gcaccaactt gatatgtctc tatttccttt caaaccaaag 88861 attccataaa taatcaaaga acctaacttt tttcttttta ttctattcga aactttagaa 88921 agagaaataa ctaaagagtt gctcgagccg gctgataaga aattttcatg tccgattcaa 88981 ccgggggaat aagaaatcta tcccaataac taaaaaacct gacctgaatg atcctgttct 89041 acgagcaaag ttagcaaaag gtatggggca taattattac ggagaacctg cttggccaaa 89101 cgatcttcta tatatatttc ctgtagtaat cttaggaact gttgcatgta ccgtaggttt 89161 ggcagtttta gaaccctcaa tgattggtga accagcaaat ccttttgcaa ctcccctaga 89221 gatattaccg gaatggtatt tctttccagt attccaaata cttcgtacag tgcctaataa 89281 actattaggt gttctcttaa tggcatcagt acctgcaggt ttattaaccg taccttttct 89341 tgaaaatgtg aatagattcc agaatccatt tcgacgtcca gtagctacaa cagtctttat 89401 gattggcact gtcgtagcta tttggttggg cattggagcg gcattaccta ttgatggatc 89461 tttaacgctg ggattattct aactctaaac aaagattaaa tagatctttt gttatatata 89521 aatactaatt attcttatct attcgttggg atctagggaa taacaaatag aatctctccc 89581 tagatccaat gaaataattg atttttgatt cttaaacaaa caataataaa ataatcgatc 89641 taaaaaaaag actaacggca ttcctagatt acaatccgct ttcctccttt cgatcgatat 89701 acgatttatt tctaggtaaa tctatagaaa aacgttgttg tagagctttt gatacctgat 89761 ctatagattt tcttccgaaa ttcttaattc tttgcaaatc ttcttgacta taattcaata 89821 aatccgagat tgtatgtata tcaattcgtt ttaaacaatt ataagctcta gcgggtaatt 89881 ccaattgatc aatgaatata tgtttgagtg taacctctct ttttactcca tttatctcat 89941 cgtttgaaag ataagataat tcttttgagt tgaattgatc tttactattt tcaacaagaa 90001 tgtcttctcg tttagtatgt aaaaaaggaa ggaatagatc tataaggttt tttgaagctt 90061 cagaaaatgc ttcgcttgga gtcaaactac cattagtcca tatttcaatg aataatattt 90121 ctgatatttc tttctcattt ccgactcgat gaacgctata attcacattt tgaacaggca 90181 taaatacagc atcgataggg aattctccaa gcttatactc ttttgaatct tttatctgat 90241 atccacgatc ttttttgatt ttgaattcta tattcaaaga gattggttgg gtaatggtag 90301 ctatgtattg tgaatcatca atggctttga ccgaagatgg taatgatata tcaccagctg 90361 ttatcttttt agggccattg atggatagaa atgcttttta aacatcataa gaatcacttt 90421 ggagtacaat ttcttttgga ttaactagaa tatcatgtat tgtttcttaa atacccgtta 90481 tcgtagaata ttcatgttta attctctcaa attttgcata tgttatactt gttcctccta 90541 cctcactcaa taaagaccta cgcagggcga ttcccacagt attaacttgt cctctcttca 90601 agggagatat accaaaacgg ccataatgaa ggcgcttact ttcgactttg gattcaatac 90661 aatcccattg aattacttta gtagagattg gtatttcatc ttgaatcata aatatatacc 90721 tcttgagttt ctgatctcat atattttgat atttagacac gtctcttctt aggaggtcgg 90781 catccattat gaggcattgg agttacatca cgcacgaaac tcagaattat accacttcta 90841 cgaatagcgc gtaaagccgt atctcttcct ggaccaggtc cgcttatcat tacttctgcc 90901 tgctccatac cttgatcaat cgacgtacga atggcacttt ctgccgcagt ttgtgcagca 90961 aatggtgtac tttttctggt acctttgaac ccgcatacac cagcagagca ccacgaaaca 91021 acttgccctc gaacatctgt aacagtaaca atagtattat taaaacttgc ttgaatatgg 91081 ataacccctt tgggtatttt acgttttcct ttccgtgagc caattctttt tggagttttt 91141 atcataatta attgattctt ggtgataaac aaattacgac tgggtactat tcaattttat 91201 cgcatatgtt attacccttg cctttgttta tgctttgggt tggaacagat taccataatt 91261 cgtcgtcgtc tacgaataag tcgacacttc tcacagattt tgcgaacaga ggtacggatt 91321 ttcatatcta taaccatgaa tctaatgaat cttatgatgg attaaacaat ctttcttttc 91381 ttgttctatc ttttataatt aataaatatt ataaagagtt tgaaaaatga tagaaatcac 91441 tactgaattc catttacttg cttatttctg agacgataaa taatacgccc tttagtcaga 91501 tcataaggac tcggttcgac ccttactcta tctcctggta atattcttat gtaattgcgt 91561 ctgatctttc ctgatatatg agttaacact tggcatccat tatccaagca tactcgaaac 91621 atagcattag gaagtgattc cgtaactgta ccttctacat caatcagatt ctgtttcttc 91681 ataattaaga agatatatcc ttcttacact attaatcaat cttatttttt taagagagga 91741 gcttcttata taaactacca aacatagcat aagatctccc ctccaatctt tctttttcta 91801 gcctcgcgat ctgtcataag tccatccgat gttgataaga ttacaatacc cataccgcct 91861 aataccttgg gtattctttt atgccccgaa tagattctca acccaggttt actaatacgt 91921 tttagagctg tcatatatgg tttttttatc tttccttaat atttcaacgt cacgtccaga 91981 agatctctca tattctctct atgttctgca atattcttta taaaaccttc ttctaataag 92041 attcgtacaa tacttttggt aacattatta gcaggtattc gagccatagt ctttcttctt 92101 gtgttggtat ttcgtagaag agtaatcata gtagaaatgg tatcgttagt catagagtaa 92161 taataattag ctatcaattc tggaatgatt gaatttcaaa ttttcttatc atttcactac 92221 tgctcgtggt acatgatact ttattgtagt tatatattaa tatttcacag attttctttg 92281 ctatcttctc tttgatattg tcattttttg ctattttaag atcattaaat cactaaccac 92341 gacagatttt gttcagatac aatcttgagc tactatgaag aaatgattaa tggttttgaa 92401 gtattttatt tcttatttct tacaatacct caggagctag cgaaactatc ttagcaaatt 92461 tatattcccg caattctctg gcaactggac caaagactct ggtccctcta gggtttcctt 92521 cctgattgat aacaacagct gcgttgtcat caaatctaag aatcatccca ttctcacgtt 92581 tcatctcttt acgggtacat actactactg ctctaactac ttctgatctt tttaacggca 92641 tatttgggat tgcttctttg attacggcta taatagtgtc accagtactt gcatatctag 92701 agttacttgt tcccagcact tgaatacaca ttaatttgcg agctccgcta ttatctgcta 92761 catttaaata actttggggt tgaatcattt attaatcaag aaaaaatata tagataatgc 92821 attatttttt gactatagga agagatagtt ccagtttctt tattcttctt attggaacta 92881 tctcttccta tttctttttt aacttttttt tgtatttgat aattttgata atcaagtcaa 92941 gataaaagat tggagagaaa aagaaagaag attctttatg attggattta ttcttctttc 93001 ttagttatcc tcaatattct tagcttattg agtatccgaa gtcactattt gagtacgtat 93061 agaaatctta taagatgcta ttttcatagc agctctggct atactctctg atactccact 93121 tatttcgtag aggattcgac ctggtttaac aacagatacc caatattccg tggatccttt 93181 acccgatccc atacgtgttt ctgcaggtct catactgatt ggtttatcag ggaagatacg 93241 tatccataat ttaccaccac gacgagcata acgggttatt gcccttcttc ctgattctat 93301 ttgtctggct gtaatccaag caggttcgag tgcttggaga gcaaatcttc cgaaagaaac 93361 gtgattaccg cgaagtgaaa ttcctttcaa ccttcctcta tgttgtttac gaaattttgt 93421 tcgtttggga ctaagcatag caaaaattta cgaaggattt gattaatgtg tttattgtta 93481 atatgaaata gaataagctg ttatagattc taatagttcc ctaaatagta tttttttcgt 93541 tagtaaatcg gataaatggt tcattttatt ctatgccttg aaatatccaa acctttatac 93601 ctaagactcc gtaaatagtc tgagcgggac gataacaata agtaatacga gctctaatgg 93661 tttgtaaagg aactcttccc tccctagccc actcaacgcg agccatctca ttaccattga 93721 gacgtcctgc tatttgtatc ttaataccct tattatttcc gtgcctagta gacaggtcaa 93781 ttgctttctt catagtcctt cggaaagcaa ctctattctc caattgtgaa gcaatatatg 93841 ttgctacaat ctttggttct ccatagggtc gagtaacatc agtcaatatt atttgaacct 93901 tacggtcttt tcctttgaaa tctaataatt ctcctatacg gcttttcaat tcatcaattc 93961 ctagatgatg atcttgaaaa aatcgatcca ggaatcctgt acatatttca atgttaatta 94021 gatctgtttt ttgttttatt tctatatgta caattcctcc ataattggaa taagcttctt 94081 tcatacgttt ctgtacatat ttttcgatta acgtacgtat ttctttatcc tcttttagga 94141 actttgcata atcttttttg tgtgcaaacc aataggaata atgcttctga tttacaccaa 94201 gtcaaaaacc aagtggatga atctttcgtc ccataaatat attcctagat ttcaatatct 94261 caaaactaag tccttttcaa gcttctttta tctcttagct ttttactgtg atttagtctc 94321 tttcaatctg atagttatat gacaagtagg ttttcttatc gggtaacctc gtccttgagc 94381 tctaggtcga aatctcttaa aataagtgct cctatctact cgagcttcac taataaaaag 94441 attagatttg cttaaaccaa gattattagt agcattagca gctgcagaaa ctattaattg 94501 taatacagga taacaggctc tgtgcggtaa aaattcgagt aatacaagtg cttgttcata 94561 tgaacaacct tgaatatggc tgatgattcg tcgcacttta gtagccgata ttctgatatt 94621 tcttgatgaa gcttgcattt ccattttctt tctctattgt tttttatttc aaagtctgtt 94681 ttctaactag cgacgagatc tcttatcatt cttagcatgt cccctaaaac tacgagtggg 94741 gacaaattct cctagtttgt gacctaccat acgatctgtt atatagatag gtagatgatc 94801 ttgtccatta taaacagcaa tagtatgacc aatcatcatc gggactattg cagaggcacg 94861 agaccaagtt actattattt ttttctcttt acgaacatta agattctgaa ttcttcgtat 94921 taaatgagta gctacaaaag gaccttttcg caaaacatgg gccatattta attgtccctt 94981 gtgtatctat tattcaatta tttttacgac gtcggagaat caatgaatta ctatatctct 95041 tagaccgacg acttttttgc ccaagtgcac tatatcccca aggagttgat ggcttttttc 95101 taccaattgg tgttctgcct tccccccctc catgtgggtg atccacaggg ttcatagcgg 95161 tacctctcac ttcgggtcgt tttcccaacc atcgcttaga cccagctttt cccaaatcct 95221 tattattaat atcaacattt ccaactcttc ctacagtcgc tatacaattc tgagatattg 95281 aacgaacttc accagagggt aatcgtaatg tagctaattg accttctttt gcaatcactt 95341 tagctgttgt accagctgct cgagctagtc gtccacctct tccaggtgtt atttctatat 95401 tatgtatgat ggtacctaaa ggtactttgg ttgaagtaga ttcttctcta tcaaatagtt 95461 attagaaaag aataatctct tcccaaactg tacgagccct tttcagggca tacagctttc 95521 tagatatcta aagacgatat ctttgttgaa tatcgttgag aataattagt aattaggtaa 95581 gttacaaatc aatctctatt tatatccttg gcctttccgc cgtcatctgg cttctgtttc 95641 ttaatctgtt cagcaacaga atgaatgaat tcacttattc acgctaacat tgtttcatgt 95701 ttttgataac ttgggagaca ctattagtaa ataaatctgt acgttgatat tcaacaaata 95761 caataaatat gttctcactt cctttttgac tctgcctttg accatcagat caactgttgt 95821 gagtaacttg ttcctaatct cttctttatt atactaaata aagaatgccc ttatctcttc 95881 tctgtgttta agattgttta accttctcca tattatctta ccttcacagg ttaataagcc 95941 ttttagtgct attagaccga atcacccgct tttgttcccc ttaagtttcc ttattgaggt 96001 ttataacaga ataataaagg attctgaatc agtgccaggt ttataggttc ttgaacccaa 96061 tcggttgttt ccgaatacgt gaattagttg ttcaaaccgc actcaaaggt aaggcatttc 96121 catttgaaat agacgctttt gggctagata ttattatatt tccaatactt attccatgag 96181 tgtgtaagat atatctcttt tcgccatcta tataatttac aagacatata tatgcattac 96241 ggttgggatc atattcaata cttcctatta ttccacaaat ctccatcttg ttcctttcaa 96301 aatcgatctt gcggtataaa cgtttgtgcc cacctcctct atgcctacta gtgataattc 96361 ctctgttatt acgcccagtt tctggattct taccatacgt taatttctta gtaggtttac 96421 atcttgttac cccttcaaag attgcgatcg atcgattgcg tgttccaggt gtgtaggctt 96481 tatatagtca aatactcata cttatctttc cttcttttat tctctttaat ttgtggttct 96541 aatcaaaatt attcatttaa gaagaatgga atagaataat tctctctaag cgtaataatc 96601 attctttttc tatatacaga atgtctgggc gttctatttc ccttcttttt cttaattgga 96661 agtcggtgac tatttatact ttttactttt acgttaaaga attgttcaat ccaatccttt 96721 atgtcagttt ttcttgattg tagagttacg ttgaaagtat actgatttta ctgcaataaa 96781 cgaatagttt tttctgtaag tacttgattt ttcaaattat ccataatatc tatatagttt 96841 tctctttatt attcaaataa taatagttgt ttctcaaatc gagtatagaa tcatctcttt 96901 gtcctcggta ctgtatagtt ttttttttat actgatttca tcgatggaac cttgtttctt 96961 ttgcatccaa caggagttga acctgtgaat tcgccaatta tgagttgggt gctttaaccg 97021 ttcagccatg gatgcgcgga tctgatagaa tgccattttg ttgtgtaacc gatcaattgg 97081 atattagaga ttattgtttg taattccata ttcaatcgaa tcaatctaca ctctagagat 97141 tgatttacat attgattcag tagaaggaat atattagttt ctgttcttct tttactgagt 97201 caatttgtaa atctctcagt aatcgataga gatgggaagg agagtattct attcttatct 97261 ttacattcta aaaattcatc caccacacta ggttggatct cttactctta gcttaggatc 97321 cgactcaatc attcactcag gatctaattg gatccaatcc taaggtccta tccttctacc 97381 gtcccgtctg atcataggat tgagagtttg aaagcaccaa cccaaagaat tcctagttcc 97441 attggtgagg aacgaggagt cttccgactc ctggaggtgg aatgcaagac ctgttgcctc 97501 gaagaggatt tgaacctcca tgcttcttct atctcctttt agcacccgat tctgaatcta 97561 gcgtgtctac cgtttcacta tcgaggcttc cctctttcct ctcctttcat ccttccctat 97621 tgattcagca tctaagacgt atttcttctt gattgtagct tgattaggag gagaagaaac 97681 cctggtgttt ctttgcttct tggatagtat tatatcccat cactctccac ttgtcaagct 97741 ttgttgatat agatcctttc agtctcagac ctagggagaa gcaaattgag tgactatagt 97801 ctatcacaat actccaaaag gagcaaccta attgtccttg aaaaggtagc agaatctatt 97861 caaaggtgca aatcgaacca gatcgatcca acctttccat gccgtgaaag aatcaaatca 97921 gagacttctc tatctgacta tctaagaaga ctatttcttg actgagaaac ctctgaataa 97981 agagtacttt gggatcttct tctagaagag gttagagata gaatgttgaa gatgaaaatc 98041 cagtctcatg tatgtatgat agctaactaa tggatcaacc gaatgagcta gatcagatga 98101 ttattaagca attgtctcta aaaatgagag ctacaattgg agttgactac catgcgcacc 98161 aacgaacata tcgaacctgc atattattcg tgttataatc tagagtttac cagatccatg 98221 aatgatagaa tcaatgattc gttgaaaatc acttaatcgt atctaacccc ttccattagg 98281 agagaattcg atgcttggaa tactgcagag gattggacaa tatccaatat catagatact 98341 cttgggaaat acttctatct catccggaat agactagacg agtcatcatt attcttctaa 98401 caagaagaat catattctac tttggaagcg tagatcccaa tattatattc tggcagaggt 98461 ttttctagtg tcggtattgc atcagtatcg agtccccaat aattgtgatt ccaaagttat 98521 ctcagactcc gtttacgttt ctattctaga tcttgcatct ggataatgcc gtattttcgg 98581 catcgctcag agtaaggaag agaagagtag agaaatgttg gtctcaaaga gagttggtgg 98641 aggaagagag tgccacgcga agattgaatt ctaccctgtc aactgggata atctctcaat 98701 tccttgtacc tatcactatt tcctaccagg acttcttaga atcatcctat tcataccctg 98761 cgggtgtagt tccttccaga atctttattt ggaagaagag aatggtggga aaagacgcta 98821 tcagattccc aaagacttga gagaccagca caagaagaga gtcttggtcg gtcaagaaaa 98881 ggattaagaa accaatacgg acaatgaata cagttccaat agccaatcca aggatagcta 98941 ctggaacgag gaatagaagg ggggggggca ttgagagaga ctccgcttaa tattctcaat 99001 atcataggat tggagcaaaa acaaagagaa gggagtgaac gattcaatgc cagtagattt 99061 caatgccagt agatgaggtc aatctccgag ttaaccgaag aaatcctatt acgatcaatg 99121 gacgaattct ttgaatcgaa gagatccctc ttacccattc ctcgattatt cgtttgattc 99181 attccttcca ccgctccaat caacaggagt aggatgacag accgtgagaa tggaattctt 99241 cctggatacc gaagcgcaaa gatctcgagc tctattgatg agcaaataga gttcttcctt 99301 tttccaagga atcttaggaa aacgaaagat ttctcaaggt tcaacgaatc ttgaatagtt 99361 ttttgaagac tactaaaacc tttccatttc gattgcaagg agtatcaaat gcttataaac 99421 cctctctctg atcgagacaa taccaggcca gggtggccaa actcagaaca acgtggagag 99481 attggggagg gcccttgtct ttccattccc aatctattcg aatgataagg acagtgagat 99541 gatcgttcta tgattcttca agatttcact ttctttctag aacataagga gaaaggctat 99601 tcgatagcaa aaacaagaag gatatatcgg cagtatcttt cacagaagtt tcttgcactg 99661 gcagaaagat tccattattc cacattcttt catgggacaa attgaaatgg gtctagaata 99721 gggtattccg tgtaatctca ataatgggat ctattaagat acctaataag gttgatgcta 99781 gtacacaaac aatcatagtt atttcaattg aactcttcga gattgaagta gatgaagaga 99841 ctgatggatc ctgtacgtaa attgttgatg tgtcactctt tccagtgaac attaacttga 99901 ttatctttgg ataataatat atagaaatga cacttgtaat gagcgctatt agaactaatg 99961 aatacaaacc agctttccat ccatgccaaa aaagatagag cttcccgaag aaacctgcta 100021 atggagggat accccccagg gatagtaaac atagaactag agagaatgtt agaacaggat 100081 cctttgtgta taatcctgca tagtccctaa tattatcagt tccagttcgt aaactgaata 100141 aagtgataca agcaaaagtt ccgagattca tgaatatata aagaaacgta taggttatca 100201 tacttgcata gccattatca ggatctgcag caagtactcc aatcataata tatccaattt 100261 ggcttataga ggggtatgct agcatacgtt tcatgcttat ttgagtaaca gcgattaaat 100321 ttcccaatat catgctaaga atagctaata ttcctaaagc aatatgccat tcaccagata 100381 gatatgagaa aatgatacgg aagatccgtg taaataaagc gagagctgct actttagaag 100441 taacagaaaa aaaagcaaca actggtgtag gagagtcaga gtcggaaaga agatcctcga 100501 tcttaccgct cattcagaac cgtgcatgaa attcttactt cacacggctc ctagttcgta 100561 agagatttct tcctcttatt gctcttagaa ccttcctcgt gtatgtttca gatctacttc 100621 ttctagatcc tctttcttaa tcattcaaga tgagtactaa ttggttgtta gcttctcgag 100681 gaatcctttc tcattaatct agagtctcca tccattctct aacctctcct tttctctatt 100741 ccaaaaccaa gaaaagatag gggaatctaa gagagatctt agataggaga aaaagaatat 100801 gattttcttc gttcttaaca ggcatgatag tattatcttg gggcgatctt ttttgacgaa 100861 tgctttcttt tactatacct acagtccctg aaggatggaa actggttgga ttgacatttt 100921 cagaaagaaa agaatcctct tctatagata atagagaaga tattctttcc gttgcatcat 100981 ctattcattc ttcgtgacac ctacccctaa taaatggaac tttgagaaag atcaagagat 101041 agagaagagt tctctattct attgggttgt acttctctct tcactctgtt ctaccttact 101101 tatgtgaaac aattcaagtg ttaagtgttt ctcgcagatt tctggggttt ttggtaagag 101161 ttagtcattc atccaattgg tgatttggag aggttcctat cctcttcaca tgtctgtagg 101221 atgtcataca gaagtcaatc aaatcttcca agagaatcta gaatgagtcg attttcttct 101281 tcgaacgaat cacactcctt catatacatc aggagtccat tgatgaaatg gaactaatga 101341 gagcttaaaa cccatcccta ccacaataaa cgtgatagaa atatagattc caatagaatt 101401 atacatttca gtagatagaa gtccatcgac tatcttatag agttgagtct ctccaccgga 101461 taatccgtat aataaagaga aaccataaac taaaatcgac gaactcgctc cacccattaa 101521 taagaatttc atcgtcgctt cattggatct tatatccttc ttcatgtatc cagataatag 101581 ataagaagac aggcttaaac actctaaggc tacgaaaata gttaccaaat catttgcaca 101641 acatagaaac atccccccta agcctgcagt taatatgaat aataagaatt ctgttaaagc 101701 tgtttttgta catcgtatgt aatcgactga tagtaagacg gataataatg aacagatcaa 101761 gaggagtagt ctgaagatat tgttaaagtt gttgatctgg agactgccgt aggcaatcgg 101821 aacagatcct gtttcccatt gacccaataa gagtattatg ctagtgacta aggatgttaa 101881 tgatattcgg taaagtagaa gtgtatcttt tcctttagat attgaatcga taataacgat 101941 tattattagg ctgataatca aactacattc tggaagaatc gatgaattac gatagaagag 102001 agaaggatcg atatttttca tagtataaat ctgatagtta gaaatagagt aattattctc 102061 gtattccgta tttagtcgat taagtcaacc aattcttgga attgttctat atctcgaaaa 102121 ccatagcatc tcaatagaga aatcttcagg taaatgaatt ggaaaagaga caagatagat 102181 agactgtttc tttctcagat atcttagaac taagtaatat tactattcta gattcttcct 102241 tgttatgcct tagatacgtc tctttcttca ttctctgatt tctgtttgtc catatgttcc 102301 tcgcgtattc tgattctcaa taccctgcgc tctataagag aaggctgaag gtttcaatcc 102361 acaatctctt ctttgtggat tgagacaaac ctgagactaa cggaaatgag caaaagcctt 102421 gttagcttcc gccatcttgt gagtctcctc tctcttgcgt acggcactac cataatttct 102481 ggcagcatcc attaattcat cacttgatct tagagccata cttcgacttg agcgttttcg 102541 acacgcgatt aataaccatc ggatagccaa agcttttcct tctgcaggta ctacctcaac 102601 gggaacccga taagtcgatc cgcctacacg tttggattct gttactatat cgggagttac 102661 cctacgtatt gcttgtcgta gaacagacag tggattccta tttgtctttt ccttgatgcg 102721 cttcatagcc tgatagagaa tttgataagc tagtgatttc ttgccatcct taaggatacg 102781 atcaaccaac atatttacta atcaattgcg ataaattgga tccgattcta tatttctctt 102841 tttgttcact atattgctcc gatgtgaaat gggtttgcag gtgtgtcaga tctcaatcct 102901 atcccaacca attgtatatt atcatcttat tttggcttct tcactccata ttgtagggtg 102961 gatccgccgg ttagtcgagg atctccctcc aaactgtacg tacgactttc atcgaataca 103021 gctttccata tgattcaata gagagagaaa tgaaggaatt ctggattgct tcggtgaatc 103081 atatttactc gttatgcatt gagtatccgt ttcccctcca ttctcttatg agtagaagag 103141 aaagaatctt gtatttcatt catcttacta agactatccg taggtttatt gatccaatta 103201 ctagatgcaa aatctttcct gaagattcag gattagagtc ccaacccgtt ctaagagaga 103261 ggagacattc aaagatctta tggatagaaa ggagtccagg tgagactcaa ccgactagga 103321 tgttagaatg gaatttgtaa taccttagat atcaagtcgt tttacacgaa tagatggatt 103381 ataatcaatc tctgtaatag caggcttccg tcccctgtca ataatctcat tctactatcg 103441 ggtcagctat ccatttaaca tatcagaaga gttgatacgg aatcattgcg atgatacaag 103501 ttacgacaat gctctacaac gcactagaac gccccttttt acgatccttt acgcctacag 103561 catctaaagt tcctctaacg atatgatatc tcacaccagg taagtcttta acccttccgc 103621 cccttacaag gacaacagaa tgttcttgca aattatggcc aatacctggg atataagctg 103681 ttacctcgaa cttggaagtt aattgaactc tggcgacttt acgtagggca gagttaggtt 103741 tttttggtgt agttgtggaa tagttgacgg atgagttggt ttcaatgtca ctctacagaa 103801 ccgtacatga gattcccacc tcatacggct cctcgccatt cgatctatat gaagaatctc 103861 tattcagcat agaaggacaa acctttttcc tctctcttca gttgttcctc gatcctcgat 103921 acataaggat ttagaaaaga atccccatgc tctactcgat ttgattctta tcaacccccc 103981 tcccctcttt gtttctattc cttgctcttc tctccctatt ctctgaatcg attgactaac 104041 ggcgtatccc ttaattaggg ataaggatag ggattaggag attctgtttc gcctttaacc 104101 ttactagaag caaatagata ttactattca ttgaatgggt tcagtattga agaattctgt 104161 atttattcca catcttgata ctacaaatac tgattccgaa tgattagtat cgaggaaaat 104221 gaataggatc tcgagataat ctcgagaaag atctttctgt tttccaggac taccgaatga 104281 acactatgtc ataatgctca tttcctctca atatgatcga gtagattaat aacagggagg 104341 gaatagagaa attcaatggg tagatttgct aatttgctaa taagaataag atataagatc 104401 cttgttctgt tttcactgaa atactattca aaaggtcgaa tatacaggag attgacgggt 104461 tagtataagc ttatccatgt tattaatcac cattcaaaag agaatccatt ctaacgagat 104521 ctttgattcg atttggcttt cattctttcg ttcgttgcga cgaggctatc tgaaaggggt 104581 tcagtaacct ataatgatat ctatctatat cagatagaga tctgatgact gcgtaaggcc 104641 tgctaatagc catgagaatg aaatggaaat agtcaagaaa accagggata tagacagaga 104701 aggaagaaga ggactctgtt atctcgatga ttttggctta gctagcccat tctgtgataa 104761 cccattggca caagtcaacc atctcgttcc cttttcgtgg acaagaatct cgggttctgt 104821 gggaagaata ttataccacg atagctcaaa ggggtcaagc tgttgttact accaatgcat 104881 gacacagatt ccgcgaatct ggttgtattc caacgttgat ccaaagaaat gacgaggacg 104941 agtatttcat ctatactatg tagtagagta atgctaatct agtgctaatt gcagatgggt 105001 ttagcaatgt cagttgtata tcccgtttcg gtagggtgcc aatgtactaa tctgatatag 105061 ttataggttg gatagggtta ctgatacgtt gggtgaaggt agcttcgatt ctatcagaac 105121 atgtctctca gaaatctctc gcattggaga cttcccgatt tcgtttcttt ttggattgaa 105181 tagggttaac aactagacta ggtgcacggc taaactttca tctccctaag cctaagatag 105241 agaacccata ggtaatatct ctgatgctag agaaagaaga gctctgtttc ttatctgtat 105301 gggagaaaca tcttcctcga gtatgactcg tcagaattgg attgggagat agaataacgc 105361 cttctggtct ggagagtcat ttcctatgga ccgaatcgtt gacaattcct attctcaata 105421 ggatttcctc gggttataac agaaagaatc tcggatttct cacttctttg tttcgggata 105481 agtactaaga agtactaata gagtgtgtgt gtggaaggga aaacgttcca gtcccagatt 105541 actcaagaga tacatactaa gctagtagaa tctagttctt tgaccatgac cattcaagtt 105601 ggttagttcc cactctcctc atgggtaagc gatcatttga tagaagagta gcaaagagag 105661 tagggatata actcagcggt agagtgtcac cttgacgtgg tgcaagtcat cagttcgatc 105721 ctgattatcc ctaagcgctg cagagatact cactctctga tatgtcccat agatacttct 105781 ctctctacaa cacgtataga tacaacggga tagatagcaa cgattttctc tggtttgaaa 105841 acggaagaag gcttcaaaaa aagcaaagta tggagagcac ttagagtgtc gattatttcc 105901 gtcattttgg taaatttcgt aatttacgta aaagatcgcc actattgaca ctatcgtgct 105961 cgtcgtaacc ctctctatcc ttgtcaaatc ccggccaggg gtctgtcttg gtcatataat 106021 caactacgtc ggatctgatg ttcttcctta agggctcccc ccctccgcca atgactaccc 106081 ttccgttggc tctcctcttt gtaacaatgt ctctgtaacc ttgagatcgg actagatcgt 106141 ctaaaacgtc tccaagccgg tagtacgtga taggcttgat accgttcctc tcagcatact 106201 ctacgtacga ttcgtacagg gatccgggta gggtctgagg gcaggagaag aggcttgtgg 106261 gattgacacg gagtggtaga ggtgggtata ctaggagtga acttcaagct aatatgaagt 106321 gaagaaacct aagttctacc tgaagataag aagacaatct gaaatcagct tcattcatga 106381 agggggaagc tataagtgat gcaactatga atcttatgga gagtttgatc ctggctcagg 106441 atgaacgctg gcggcatgcc taacacatgc aagtcgaacg ggaagcatcc tcgtggtgtt 106501 tccagtggcg gacgggtgag taacgcgtaa gaacctgccc ttgggagggg gacaacagct 106561 ggaaacgact gctaataccc cataggctga ggagcaaaag ggagggatcc gcccaaggag 106621 gggcttgcgt ctgattagct agttggtggg gcaatggcct accaaggcga cgatcagtag 106681 ctggtctgag aggatgatca gccacactgg gactgagaca cggcccagac tcctacggga 106741 ggcagcagtg gggaatcttc cgcaatgggc gaaagcctga cggagcaatg ccgcgtggag 106801 gcagaaggcc cacgggtcgt aaactccttt tctcggagaa gaagcaatga cggtatccga 106861 ggaataagca tcggctaacc ctgtgccagc agccgcggta agacagagga tgcaagcgtt 106921 atccggaatg attgggcgta aagcgtctgt aggtggctct ttaagtccgc tgtcaaatcc 106981 cagggctcaa ctctggacag gcggtggaaa ctattgagct ggagtacggt aggggcagag 107041 ggaatttccg gtggagcggt gaaatgcgta gagatcggaa agaacaccaa aggcgaaggc 107101 actctgctgg gccgacactg acactcagag acgaaagcta ggggagcgaa tgggattaga 107161 taccccagta gtcctagccg taaacgatgg atactaagcg ctgtgcgtat cgacccgtgc 107221 agtgctgtag ctaacgcgtt aagtatcccg cctggggagt acgttcgcaa gaatgaaact 107281 caaaggaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt cgatgcaacg 107341 cgaagaacct taccagggct tgacatgctg cgaatccttt tgaaagagag gagtgccttc 107401 gggaacgcgg acacaggtgg tgcatggctg tcgtcagctc gtgccgtaag gtgttgggtt 107461 aagtcccgca acgagcgcaa ccctcatgtt tagttgccat taatgagttt ggaaccctaa 107521 acagactgcc ggtgataagc cggaggaagg tggggatgac gtcaagtcag catgcccctt 107581 acgccctggg cgacacacgt gctacaatgg ccgggacaaa gggtcgcgac cccgcgaggg 107641 caagctaacc tcagaaactc ggcctcagtt cggattgcag gctgcaactc gcctgcatga 107701 agccggaatc gctagtaatc gccggtcagc catacggcgg tgaatccgtt cccgggcctt 107761 gtacacaccg cccgtcacac tatgggagct ggccatgccc gaagtcgtta ccttaaccgt 107821 agggaggggg atgccgaagg cagggctagt gactggagtg aagtcgtaac aaggtagccg 107881 tactggaagg tgcggctgga tcacctcctt ttcagggaga gttaatactt atttggtact 107941 ttggtcttct atccactgtt tcgcactccg aaactccgag agaagtgatc cacagaatcc 108001 acttatgggt ggggttatct tgtcttgaca gtgaagcaag accaagtcca tgagcttgtt 108061 ttattctctc aggtcggaat aagctgagag tgatcccatc cccatccttc cttagggagg 108121 gatgacggat aaggataagt tccctctcgc ctccggtatt gtaggcccct cctattccta 108181 atagggatag ggaggactgc atgacgggct attagctcag ttggtagagc gcgcccctga 108241 taatcgcgtc gttgtgcttg ggctgtgaaa gctttcagac acatgggtag tttactgtgc 108301 tcatctgcgc ctgacccgaa gatggagatc atccaaggaa cgttagcatg gcgtactctt 108361 cctgtctcaa ctaggtcgtg agaacgaatt actcttcagg aagatagatg aggcgattca 108421 ggtgagatct gatgatgatt caacccgcta ttcactcgtg ggatccgggc gatccaggga 108481 gggaggacca attctcgatg atggctcccc tcttctcggg aatctataca ctccttatca 108541 gtgtatgggt ggctatctct cgagcacagg ctgaagttca gcctagaggt gagaaacgga 108601 gcacctgatg acgcatattc acggaccagg aactacggaa tcacccttct cattctaggg 108661 tgacggaggg atcatactat ccgagcctat tctgctctct ataaaggtcc gtagaagtag 108721 caatcaatag aacctctctg gaggtaagga atctagaaag gggggacaag tttcagaaac 108781 aatccctgga ggggccagga gatcgggcct agacatagga gcgattcctg atgcaaatca 108841 ctcccgggtc cctcggccct atcaggtatt ccaaacagcc tccagatctg atctatggaa 108901 tgggatgtct ttattcatcc ttcaatgata ggagcagcag gttcgaagaa ggatcctaga 108961 gtgtccaggg ttgagctagg agggagggtc tcttaacgcc tccttttctc tcccattgaa 109021 gtccctttcc agagcataaa ctcgctgttg gcaaagaaga gaggaagggg aaacaagcac 109081 acttggagag cgcagtacga cagagagttg tatgctgcgt tcgggaagga tgaatcgctc 109141 ttaagaaaga atctcttgat tctctattct tatgggatag tggtgtaggt gcgatgattt 109201 acttcacggg cgaggtctct ggttcaagtc caggatggcc cagctgcgct aaggggaatt 109261 agaagaagca tctgatccat tcctgtatgc ttcacttggc ccgggcacag gggatatagc 109321 tcagttggta gagcgccgcc cttgcaagtg ggtcgttgcg cttacgggtt gggtgcctaa 109381 tcatctaggc ggtagtgata gtatctttta cccgaaccgg tggctaactc ttcctcagga 109441 atggggagga ggaccagaac atgccactga tagactctac tgggacaaag atgagctgtc 109501 gagaatgtag gggggtagga taggtggttg gttagatcca gtatggatcg tacatggacg 109561 gttgttggag tcggcggctc tcccagggtt acctcatctg gatctctggg gaagagggtc 109621 gagctggccc ttgcgaacag cctgatagac tatctctctt caactccctg agcacagtgt 109681 ggcagaagga actagaatcc atggaccgac cccatcgccc ccaccccgta ggaactacga 109741 gatcgcccca aggatgctga aggcatccgg gggtcacaga ccgaccatat aacccgatcc 109801 gagaagtgga acgctttagc tgtccgctcc ggttggcgga gagggtcgga gaggggcaac 109861 tactcattat tgagactaaa gagtcgggca aagaagggaa agggcaagaa ccttagcttt 109921 tggttctcct gtagctcggg agtatctagc tagaagaatc cctagaatgg gatctcaacc 109981 tggcgccttt caattcgaga agagttgctc ctcggagagc acggtacgat gaaagttgta 110041 ggccgtgttc gggggggagc cattgtctat tgccggcctc catggtagaa tcaatcaggt 110101 aggcctgaga gatggtagct taccctgtgg cggatgtcag cggttcgaat ccgcttatct 110161 ccagcctgtg atccgagtta atagaatcgc atacattagt catatattag tatttagtat 110221 ggaataatct tgatcccctg tgctgtgggg aggttgatct agaatctatt ctctatggac 110281 gttgatagga tctctgtatt tagcacctta agatggcaag catagtattc aacgacgagg 110341 ttcaaacgag aaaaggctta cggtggatac ctaggcaccc agagacgagg aagggcgtag 110401 taaacgacga aatgcttcgg ggagctgaaa atgagcattg agccggagat tcccgaatag 110461 ggtaaccttt tgaacttctg ctgaattcat aggcagacaa gagacaaccc ggcgaactga 110521 aacatcttag tagccggagg aagggaaagc aaaagcgatt cccatagtag cggcgagtga 110581 aatgggagta gcctaaaccg tgagaacggg gttgtgggag agctatgaga gtcctgtgtt 110641 gctaggcgaa gcggttgagt actgcacccc agatggtgag agtccagtag ccggaagtac 110701 cactggctta cgctccaacc cgagtagcat ggggcacgtg aaatcccgtg tgaatcagca 110761 aggaccacct tgcaaggcta aatactcctg ggtgaccgat agcgaacagt accgtgaggg 110821 aaaggtgaaa agaaccccca tcggggagtg aaatagaaca tgaaaccgtg agctcccaag 110881 cagtgggagg agaattggat ctctgaccgc gtgcctgttg aagaatgagc cggcgactta 110941 taggcagtgg cctggttaag ggaacccacc ggagccgtag cgaaagcgag tcttcctagg 111001 gcaattgtca ctgcttatgg acccgaacct gggtgatcta tccatgacca ggatgaagct 111061 tgggtgaagc taagtggagg tccgaaccga ctgatgttga aaaatcagcg gatgagttgt 111121 ggttaggggt gaaatgccac tcgaacccag agctagctgg ttctccccga aatgcgttga 111181 ggcgcagcag ttgactggac tatctagggg taaagcactg tttcggtacg ggctgcgaga 111241 gcggtaccaa atcgaggcaa actctgaata ctagatatga cctccaaata aggagggaga 111301 aagggtcaac cggtgagacg atgggggata agcttcatcg tcgagagggg aacagcccag 111361 atcaccagct aaggccccta aatgaccgct cagtggtaaa ggaggtaaga gtgcaaagac 111421 agccaggagg tttgcctaga agcagccacc cttgaaagag tgcgtaatag ctcactgatc 111481 aagcgctctt gcgccgaaga tgaacgggac taagcggtct gccgaagctg tgagatgtca 111541 aaatacatcg gtaggggagc gttccgtcct agagagaagc atcagcgcaa gcaggtgtgg 111601 acgaagcgga agcgagaatg tcggcttgag taacgcaaac attggtgaga atccaatgcc 111661 ccgaaaaccc aagggttcct ccgcaaggtt cgtccacgga gggtgagtca gggcctaaga 111721 tcaggccgag aggcgtagtc gatggacaac gggtgaatat tcctgtacta ccccttgttg 111781 gtcccgaggg acggaggagg ctaggttagc cgaaggatgg tgatcggttt aaggacgcaa 111841 ggttaatgga cccgtaaaag ggaataagag gtagagaaaa tgcctcgagc caatgtccga 111901 gcactaggcg ctacggcgct gaagtaaccc acgccacact cccaagaaaa gctcgaacga 111961 ccttcgacaa gtgggtacct gtacccgaaa ccgacacagg taggtaggta gagaatacct 112021 aggggcgcga gataactctc tctaaggaac tcggcaaaat ggccccgtaa cttcgggaga 112081 aggggtgcct ccttacaaac aaaggaggtc gcagtgacca ggcccaggcg actgtttacc 112141 aaaaacacag gtctccgcaa agtcgtaaga ccatgtatgg gggctgacgc ctgcccagtg 112201 ccggaaggtt aaggaagtcg gtgatctgat gacagggaag ccggcgaccg aagccccggt 112261 gaacggcggc cgtaactata acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc 112321 gacccgcacg aaaggcgtaa cgatctgggc actgtctcgg agagagactc ggtgaaatag 112381 acatgtctgt gaagatgcgg actacccaca cctggacaga aagaccctat gaagcttcac 112441 tgttccttgg gattggcttt gggctcctct tgcgcagctt aggtggaagg caaagaaggc 112501 tcctttccgg gggggcctga gccatcagtg agataccact ctagaagagc tagaattcta 112561 accttgtgtc aagacttacg ggccaaggga cagtctcagg tagacagttt ctatggggcg 112621 taggcctccc aaaaggtaac ggaggcgtgc aaaggtttcc tcaggctgga cggaaatcag 112681 ccttcgagtg caaaggcaga agggagcttg actgcaagac ctaccagtcg agcagggacg 112741 aaagtcggcc ttagtgatcc gacggtgcca agtggaaggg ccgtcgctca acggataaaa 112801 gttactctag ggataacagg ctgatcttcc ccaagagttc acatcgacgg gaaggtttgg 112861 cacctcgatg tcggctcttc gccacctggg gcggtagtac gtcccaaggg ttgggctgtt 112921 cgcccattaa agcggtacgt gagctgggtt cagaacgtcg tgagacagtt cggtccatat 112981 ccggtgcggg cgttagagca ttgagaggat ctttccctag tacgagagga ccgggaagga 113041 cgcacctctg gtgtaccagt tatcgtgccc acggtaaacg ctgggtagcc aagtgcggag 113101 cggataactg ctgaaagcat ctaagtagga agcccacctc aggatgagtg ctctcttatt 113161 cttactcact cggaagactc tggacgcaga aagagccgga gcaacgacgg attacccgtc 113221 ctggcaaact gtgaaacgac ggaagtcttt gatctgagaa tccaagataa ggtcacggca 113281 agacgagccg tttatcatta cgataggtgc caagtggaag tgcagtaatg tatgcagctg 113341 aggcatccta atagaccgag agatttgaac cttgttctcg cataacctga tcaattcgat 113401 taggcaatgc cacctattat tcgattgtct gacaacatca gcatcagcct aggatagaag 113461 tcgatccaag agttccgccc cttctccgtc catcgaaggg atggaaacct gggtcataat 113521 aatgttctct gcctggaagg tacactattc cctctggtgt acctttcgga attagagcct 113581 tctaagaact aaccaataga tacatagata cacttcttcc ttcttttcct tttcgttcac 113641 aggttgatat tctggtgtct taggcgtgga ggaaccacac caatccatcc cgaacttggt 113701 ggtgaaactc taccgcggta acgatactgc agggggagcc ctgcgggaaa atagctcgac 113761 gccaggatga gagaaagggc tcaatccctt ccattctccc ttaaaggtca tctccttctc 113821 cgttcgagac cccatggata ttctagtagc acctccgtct ttctcccttc atgagaatag 113881 aatcccctct ctttctggct gggttggggg agaagaggat cctaagatct ctaactcgga 113941 atggatggag agaggaaagg ttgcctccat tctagtctat tctagaaaag ttccaccgga 114001 acagatccag tggagacggg gcctgtagct caggggataa gagcacgtgg ctacgaacca 114061 cggtgtcggg ggttcgaatc cctcctcgcc caaaatcatc catccctaaa cgtcccttat 114121 ctctcactag tcaataggca agcaaatagt atgattagga ttccaggcgc aaagatgcaa 114181 agccaaagaa aaccatggga cttgatcctc cacaaaggca tagctctttc ttcgccggat 114241 atgacccgac tctctcgtat tgatttgttt ctcttaagcg gagatcttcg aagaagcaac 114301 gttaaactct cgggtcctgc ccttaaaagc attcgattgg attctctaat agggaaggga 114361 ggatattgat tacccattct taattctata tcatgagaaa gtaccttccc cttctttggc 114421 gtatatgaat gaagtattat tattatccta ctgaagagaa gcatcaatat ctacatgatt 114481 caaactatcc tctattccac tggaaataga tgaatctccc cgggtagggt ttgaacctac 114541 gaccaatcgg ttaacagccg accgctctac cactgagcta ccgaggaaga ttgcagagcc 114601 catatacatt caaagactct tgtttcttga gccgcctttt aacccttaac ctctaagacg 114661 ttcattattc gaagacacaa agtttccttc gggacttcat aaacctcgcc tacaccctaa 114721 ctcttattat aggacgaagc ggtagtgata gcaatcccct ctgcctcctc ggatagagcc 114781 cctgattcgg ggggggggcg atcaatcatg actaagatct tacgcaccag cccccccccc 114841 cctaagatgc atatcgatca atcaacaatc actagtttct atttctcctt ttccgagaaa 114901 catagtagaa tagtcacagg attcttctac ttctagaagt agaagcaata tcttctcttg 114961 caaactctta ggaaacgaga gaaacgaaaa caattaacaa tggaacgatc ccaatcatta 115021 atccgaataa ataagaggat actctcccgc cttccccgta tctggcactc tctcccccta 115081 agaaactcga gataccgata ccattaatca ttccgtcaat tacccatttg tcaaagattg 115141 aaataagctt gctcacagaa cggatacctc ttatgaagaa tctatcataa taataatcaa 115201 tgtaaccccg gttttctgac caatctcgaa gaaagaataa gaatgaatcc aaccatcgtt 115261 tagatatata tgggtttttt ataggtccat aaataatgta ggaaatagat attccaacta 115321 cggataaact aactgaacct agcgagtcta ttaagacttg tattaaatta tcaggattct 115381 cttcatattg agaaatacct ctaagaggaa ttaaccactc cgagagcaaa tcaagaccag 115441 tctctgtttg atagaaatga attcctatca atccactcaa tagtgttggt attgacaaga 115501 tcatcaaagg taagatcatc aaagagtttg actctttagg gtagatagac tccgaagaat 115561 aagaggcttt agaagtaaag gaattctttt ctttatatcc tttgataggt tttttattcg 115621 aatcaattag ttcattccat aaagatgaag aaagattact cttttcttct gtcatttgag 115681 tattacccca aataatattg ttattgacaa gcgaaagagt atttctatca ctacgattgg 115741 cacggaactc accttcaaat gtaagaagat acatacgaaa catgtaaaac ccagtcaaaa 115801 ccgctgtaga tgaagctata catccaaacg aagaagaata gatccaactc tttacaataa 115861 tctgatcttt agaccaaaaa caggctaaag gcggtatacc acaaagagac aaagtaccta 115921 gtaagaaagt tgttccagtg attggcatat atcttcttaa accacccatc aagagcatat 115981 tctgattctt attgggagaa tatccaacaa atcgttccat cgagtgaatc actgaaccag 116041 accctaaaaa taataaagct ttagaataag catgtgtaat aagatgaaat aaagcagatt 116101 cataagcacc gatccctaaa gccaaaatca tatatcctaa ttgagacata gtagaaaaag 116161 ctaaaccctt tttgagatct ttttgagcaa gagctaatgt tgcccctagt agggcggtta 116221 ttccgcctat ccaagatatg atattcatgc ttaaaggtag atcttcaaaa agcttaaata 116281 tccgagctac aaagaaaatc ccagcagcta ccatagtagc agcatgtata agagctgaaa 116341 taggagtggg tccctccata gcgtcggaca accagacatg cagtgggaat tgtgcagatt 116401 tagctaccgg acccaagaat agtaagagag cacatatgtt agcaaaagaa taattaacac 116461 tattgttaga agttaattca ttgaatcaat cacacgattc acggatctca aaactaccag 116521 ttatccaata tgtccctaat atgcctaata ataatccgaa atctcctata cgattagtta 116581 caaaagcttt ctgacaagca ttggcggcac ttggtcgtgc aaaccaaaag cctatcaaca 116641 aataagagca catcccaacc aattcccaga atatgtaaat ctgtatcaaa ttgggactaa 116701 cgactaatcc taacatggaa gcagcgaata aatttaaata aacaaagaat cttacatatc 116761 cttgatcatg atacatataa ctatcactat aaatcataac caaaatacct acagtagtaa 116821 ctagtattga catgatcgaa gtgagtggat cggaaacaaa gcccatcttt atagaaatat 116881 catcatttaa aatccatgac catgaatgct cataagttga atggttaaca atctgttgct 116941 taaatagatt aaaagagata tacattgcta cagctaatgc aagaatgcta aacatagcac 117001 atacacgacg aaggtcttta gttacttttg gaaagataaa cgaaactaat ccaatcaagc 117061 cggaagttac gaatggacat gcaggaacaa tccaggcata tgtatataat gattccataa 117121 acatagatta tattgtgaat atcttttaga ttaaaaagga aataaaatat atacaaatat 117181 caaagacctt cccttataga gaaaaataat ctagttccta tttgtctcaa ttgaatcttg 117241 atatcgaaac ttgacaagaa tcaaatacgt cggagatact tatttaagtc tcttttttaa 117301 tcttaataaa attgattaac ttatcttatt gactcgtcta aaacaaaagg accatgatga 117361 ataaatatgc aataattgat actgggggca aacaactccg agtagaacca ggaagatttt 117421 atgacattca ccgttttgca ttaactgatc ctaacatctt aaggcctaat accaagatat 117481 cgatgtatag agttctatta atttgtgaag gatctaagat tgatcttgga cacccttggc 117541 tgagcaacgc aattgttagg ggacgggtcc tacatccttg ctttgataaa aagagaataa 117601 tgcaaaaaat acattctaag aaaaaaacac gtaagaaatt gggatacaga caagatttca 117661 ttcgatttgt agtagattct atttcttcaa atcgaaacga atcgatcggt tagttaatac 117721 cactatcata atgagatgag taataagatt ctatttgaag aactaatatt ctcgattatt 117781 atcctttctt tcctttctgt ttgtaattat ttttctttta attatattca ttctatcaat 117841 acgtatcaat cttgttatag aatctgtctc aatttcaaca atagacccac aaaaatatac 117901 ttcgttatgg cagtcccaaa gaaacgtact tccagatcaa agaaaaagat ccgtaatcat 117961 atttggaagt ctaaaatcga tcaaatagca tcaaaagcgt tttcattagc tcgatccatt 118021 ttgactggcc gttcaaagag tttttattat atagttacga atcaaagaaa ttatgaacca 118081 atcaaaaaag attgaagatt aataggaaaa caaagagatt ctagtaataa atcaaatagg 118141 tgatatggtt caaaacaaac gcataaactg tctgtctggt gaaagggaga atccagacag 118201 aatgattatg acaagacgcc agttccaata aatattctga atagggcata gatcaaaaga 118261 tgattcaaat aataacaaga attttaaaga agaaaaatag agtattgcta aaagatatcc 118321 tccttatgtt taaacagtag caataagatg ttgatcaagg aaaatagaag aataactctt 118381 tcaatctagt ctttgaagat aatatatacg atagagatct tgtactccac taagagatca 118441 attcaatgaa taatcgatct aagataagat tgattcagaa gtaagatctt taaagtttga 118501 caaccttcca tcttttgaat cagtgcagaa agagaaagaa taaatattat aagcaaaaaa 118561 cagagaaatt atagaaaaac gtctatcacg atcatatcga taatatagaa ggagaaaagg 118621 tttattttct ctatatcttt ctaaataaga agatcgtaat atatctcatt caatgctata 118681 ctttactctg aaaaaccaaa atattctatt tgttatgata ctatttattt atttaccaaa 118741 aggattgtat cctcttcgga atagcaggat ttgaacctgc gacctccatc accccaagat 118801 ggcacgctac caagctacgc tatatcccgt tcttttcgta tcattatagc actgaatcct 118861 aaatcaagct tataaatata tagaaataat ggttgcagag agcataaggt ccgctgtcta 118921 tgcatccttt tatctattct ctccgtaata tcaatggaag atctggaaga ttgacgagtc 118981 acttaataag atggtattat tatattcgat tagcaataag ccgctatggt gaaataggta 119041 gacacgctgc tcttaggaag cagtgctaga gcgtctcggt tcgaatccga gtagcggcat 119101 tatttcgcga tcaaagatga ccatcaacta ctagaggtat tagaattatc ggctttctag 119161 acaagaaata gatgcaatcg ttttaaatta tatactttca atctttaaga gtaagaatac 119221 tgattgaatc ttgttaattt actggatatt tccattatgt acaccaatat agaatataca 119281 tttactcaca tctcttttct tatacttctt tccgttactt tgtttcagtg gataagttta 119341 ttttttaaga tagagagatt aggccgtttg agcggaaaag gtatgataat tgctttcatc 119401 tgtataacgg gatctaccac gattcgttgg attcaatctc aacatctgcc attgagcaat 119461 ttatatgaat catctatgtt tctctcgtgg agcttttcct tactccatat aattctagca 119521 gttaagaatc agaatgggtg gtcaggagca ctcacagcac caagtgccat gttgactcat 119581 ggtttcgcaa ctctatgtct ctcggacgaa atgcaacaat ctacaagatt agtacctgct 119641 ttgcaatccc attggttaat gatgcatgta agtatgatgc tgttaagtta tgcaacccta 119701 ttatgtggat ctttgttagc aatatcttca ttggttattc tctatgacaa aggaacagat 119761 ccttctgtgg gcaaaagaag tcagaatcta ttaactttta tcttcttgaa aaaaattgat 119821 aaagattctt ataaacaaga agataataat ctaagaaaat ctttttattg gttatcttgg 119881 aattcgaaga aatgccagtc aatccaccga ttcgatcaat ggagttatca acttataagc 119941 ctaggatttc cctttctaac tattggtatt ctttccggag cagtgtgggc taatgaagct 120001 tggggatctt attggagttg ggatccaaaa gagacttggg cattagtcac ctggatcata 120061 tatgctattt atcttcatat caaaataact aagagctggc agggaaaggg gccggcaatt 120121 gtagcatcta caggattctt cctggttcgg gtttgtttct taggggttaa tctatcggga 120181 atcggtttac acaattatgg atggttgatt tagggatgga gtgagaaaga gtgaattaac 120241 aaaagaaaaa tacctgaaac catttgctca aatatagaat caattagaat tagtctcttg 120301 agatagggaa tcgtcatatc ggttccttat tcctatttat tgaaagaata ataggacttt 120361 acttattgtt ttattaataa taataggaat aaagggtttc tatccagtag taataataga 120421 acaatcttca ataatagatt ctagaactga aaaaaaaaaa gagtctaatt tctgattcaa 120481 caaaatatta tctatttaaa gaaatcatat tgagataaaa tagatattat cttcttattc 120541 catgaaggta aaacaatatt aggatacaac ccaataccaa taattgggag aaagaaacaa 120601 attaaaataa atacctctcg cggtccaata tcgatcagat acggatctga ataattggat 120661 attctatagc cgtaaaatat tctgcgtaac attgatagta aataaatcgg agtcaatatt 120721 ataccaatac tctcaactat tgtaactaat aatcgaaata gtattgaata actctgatta 120781 gttattattc ctaagaacac catcaattct gatacaaatc cactcatgcc cggtaatgca 120841 agagatgcca ttgaaaaaat actaaacata gtgaatatcc taggcattcg gatagctatt 120901 cctcctagtt gatcgagaga atatgtatgt gttctatcat aacaagtccc tgcaaggaag 120961 aataatgctg cacctattaa tccatgagag atcatttgta agacagctcc atcaagacct 121021 atatttctta aagaaccaat tccaattata acaaaaccca tatgtgaaat cgacgaatat 121081 gctattcttc tcttaaggtt ccgctgactt aaagaaatca gcgatgcata cacgatttgg 121141 aacgctccaa atagaactaa ccatgaagca aatatagaat gagcattagg caacagttcc 121201 atattgattc gaatcaatcc atatcctccc atttttaata acactccagc gagcaacata 121261 gaggtactat aatgtgcttc gctatgtgtg tccggtaacc aagtgtgaaa gggaaaaatt 121321 ggcaatttta cggcataagc tatcaaaaat cctaagtaac agagaacttc caatgccatc 121381 ggatatgatc tattagttaa gtcttgaata tcaaaagaag gtaccccagt cccataaaaa 121441 ctcatggtta gagaccctac taagaggaat atagaaccac cagctgtgta tagtataaac 121501 ttagtagctg aatagagacg tctcttacca ccccacagac atagaagtat ataaattggg 121561 acaagttcta attcccacat gagaaagaag agtagaatgt ctcgagaaac aaataatcct 121621 atttgacccg tatacatagc taacattagg aaatagaata agcgtgtgtt ctgggtgatt 121681 ggccaagctg ctaaagctgc taaagtagta acaaatcctg ttaatagaac aagacccata 121741 gagagtccat caatgcctaa aacccagtgg aaatgaatag aacctatcca attcgacttc 121801 tctatcaatt gaaccgattc atcatcgata cgaaagtgac aatagaatat atagagaatt 121861 agaagaaatt ctaatataca tatacctagt gtataccatc gaacgattcg gtttccattt 121921 cccgggagca ttggaaacaa aatactggcg aaaattggta aaagaacaat tgttgttaac 121981 caaggtacat tactgatcat ggatagaaag atttagtgaa gaaaatgagt aaataaagat 122041 tgagaaatga taaaatactg actcatactc aaaggcattg agctcaaaga tttgagtatg 122101 agttaagaag agaatgaatc ttatcttttg gattcttctc aaatggcaga ttagtatcct 122161 agacccatac tacgagtcgt ttcagctccc aaataaacac gtacacttaa gaaatctgtt 122221 ggacaagcgg attcacatct cttacaaccc acgcagtctt ctgttctagg agcagaagca 122281 atttgattgg ctttgcaccc atcccacggt atcatttcta atacgtctgt cggacaggct 122341 cttacgcatt gggtacaacc gatacatgta tcatagatct ttacagaatg tgccattgaa 122401 cttttttact cctattatat tattgaatat actttattat caaaatgatt agtatatgct 122461 gcttcaaacc ctattcttca ttttttttag gattctttta tttctttacc ttatatctag 122521 tagtagatac tttgtaagat cgagaccctt cttgttatgt cgttccctgg aattaatgag 122581 ttatttaata tatagataat cacgatacaa aacaaatcta atgaaagaaa gattgagata 122641 gatacgacat gattcttcag tttttagaaa atagaccgaa tagagacaat attaatagta 122701 ccattctatt agtagaatat aaatggatta atcgaacatt ggatcattca aaatattatt 122761 ctttgactaa tcaccatttt aacagatcaa attgatcaat acgagtcgat cttctattcc 122821 gttgaataac tagagcaatg gctaaaccaa tcgtagcttc agcagctgca atagctatta 122881 ggaataagga aaatacttct ccctttagct gttgattgtc aaaagaatta gagaatatta 122941 caagattgat attaactgca ttaaggatta actcaagaca catcagtgct ctaactatat 123001 ttcgacttgt aattagtcca aagaaaccga tgcaataaag aaaggcacct aggattaatc 123061 catttttgac cataatctat taactcccca cctagatgat ttcttacctc tttaaaaaaa 123121 ggatcgattt gatattaaga gtaattatta atcacgaaga taaagaatta tccctggacg 123181 atgaagcaga ctctttgttt gtctcgacta tttgttgatt gcgagctata gtaatcgctc 123241 ctattaaagc aatcaaaaga agtattgaaa ggagttcaaa cggaactaga aaatctgtta 123301 acaattcata tccaatcttt tggatgttgt ttctagaaat tccttcaaca atagtctttg 123361 cttgttgatt cagagagata ttggaccatt ctgtatctcg aatcataatt gttaataata 123421 ggaagagacc tgtacaagct cctagagtag tagcatctcc catattccag gaagtggatg 123481 aagaatcaga atctattggt ttatcaataa ccattacagc aaatacaatt aagacattta 123541 tagcccccac ataaataagc aattgtgcag cagctacaaa atcggcattc aatgtgagat 123601 aaaacagaga tatacagata agaaccgacc ctagtagaaa ggcagaatga actacattag 123661 caagtaatac aactcctaag cttcctgata ggatgcctaa ttctattaat attaatatga 123721 ttccatgaat tgattcagat agattcgttt tacaagatca ttcagatatt tcttttccaa 123781 catactattc gtaaagtaat aaaaagtatg gattattata tctatctata gagatgatat 123841 atagtctttt cttcctactt gtcccagctt ttagtatttt tattgaatat tatccttttc 123901 aaatctaata gaaagaataa agagcatagc tatccccatc agatagggat aacttgaaaa 123961 tactcaataa tatctcagtt ttgattgttt ctggaaagtc aatcaatcct ttgaatcaga 124021 attcaattga tattgtgagt gatagtgcga tcattagagt gcctttccat gactcccata 124081 gataaataat tgagagcgta aattgtttgg attgcagaat cctgggagac cgaaaggggt 124141 aaacgtccca aagacatttg atcataattt aattcatgac gatcataagt tgagagttcg 124201 tattcctcgg tcattgacaa acagttagtt ggacagtatt cgacacaatt cccacaaaaa 124261 atgcaaaccc caaaatcgat actgtaactc tttagttgtt tctttctaat attctttatt 124321 aattgccaat cgacaacagg caaattgatt ggacatactc gaacacaaac ctcacaagca 124381 atacatttgt caaattcaaa atggatccgt ccgcggaatc gttcggatgg tataagtttc 124441 tcataaggat actgaatagt tgtgggtaaa cgattcagat ggtctagagt aaccgtgaat 124501 ccttgaccaa tatatttagc agcttgaatt gctcgttgac cataatcttg aaatccgatc 124561 atcatagaaa acatatgata aataacctta gaaggaatgc tcagttgtta caaatttaat 124621 cgatttcaat tagaattagt agcaatggaa atcaactatt attaagaaat agctgaaatg 124681 ctgctgtcag taacagattt cctaaagcaa tgggtaaaag gaatttccac ccaagatcta 124741 ataaattgtc tattcttact ctaggcaaag tccatcttgt catgatagaa acaaataaga 124801 aaaaatagga cttagctaat gtgataacaa tttctagtat tacactaaat gtatcaagag 124861 atccaccgct agaatcccag ggaaggcaaa ttctttcaat ctgtggaagg aatggaatcg 124921 atagatccca tccacctaaa taaagaactg ttacaaataa tgaggagact aataggttca 124981 ggtaagaacc tacataaaaa agaccaaatt ttatccctga atattcggtc tgataaccag 125041 ctaccagttc ttcttctgct tccggtagat cgaatggcaa cctttcacat tctgctaatg 125101 aagatatgaa aaaagcaata aaaccgatag gttgacgcca caaattccac cctaaaaacc 125161 catattgaga ttgtgcttga actatatcaa ttgtacttaa gctattagat aattatagtc 125221 ggtggtgaag ttaagttatt ggtaccacct ctaattcaaa accgtacatg aacctgagaa 125281 ttcatacggc tcctcgtagg tttcgtaaag aaagcttgct aataacctaa ttttaccctt 125341 ttaaaatatc ttaaaaggac ttataagatt agccaacggg attctgtttg ccacgagtaa 125401 aggcttctga atccatctca accttgttag ttccttaatt agtataaccc aagacaagac 125461 ttgttgtagg tttttcttga tctcaatagt tcccgaatta attctactat cttactcgaa 125521 gagaattatt gatactatac actatgccaa taattctctg caatagttag tagaacggaa 125581 ctattgaact gtggaacttt cgaaaggatt acttcgttcc aaatagtcat aattaggata 125641 gattgggata tactcaagat cggagtctta aagatttact gcttagtcat ccctaccaaa 125701 gctaagtctt tactcaacga gagctagaag aattctgatt agagtattct ttttcctatc 125761 ttttcttacg attcttttct gtatattagt gtttttgccc atatctaact ttgcttatga 125821 aactccatct tttcttgtcc ccgcttcagg tttcgagaat tgttcgatac cagggattcc 125881 ataatttcaa ctgcctggac acgctttcac tcctgtatgt agaggttggg actcaattca 125941 agtactgcaa gatgctgttt gatattaccc aaatgactat ctagttactt tgttaaagaa 126001 atgaatgaag aataggcaat attcttttct ggttactatt taacgaatca cacgtaggga 126061 tactgataat atgcataaag ctaaaggtat ttcgtaacta atagattgag cagcagctct 126121 aagaccaccc aaaaaagagt atttattatt tgaaccataa cctgccataa gaagacctaa 126181 gggaacaata ctcgagatag cgatccatag aaaaacacct atatttagat cagcgaggat 126241 aacatcttgt ccaaaaggta taatcaagta gctcataaag actggtatga ctaccacagc 126301 tggtccgatg ttgaataacc aagtatctcc tctggctgga acaatatctt cttttaataa 126361 aagcttgatt ccatctgcca tagattgaat gattcccaac gggccagcat attcaggtcc 126421 gatacgttgt tgtattcctg cggatatctt tcattcaagc catacaataa ctaatactcc 126481 tattgttact cctacgataa ggctaaatgc agatattaga atccggatca attcaaaaga 126541 atcgcttgaa actcctaatt catcaaacag atcaaatatt cgcccttcaa aatcttttgt 126601 accagttacc atattaacga tcaacctccc ccataataat atctatacta cctaatatcg 126661 tcataatatc agcaagtttc attcctttca ctaatcgagg aagaatctgc aaatttataa 126721 atcctggcgg acgtattttc catctccagg ggaagatact accatctcca attaaaaaga 126781 cccctaattc tcctttgggg gcttctactc ttacataatg ctcttgcttg ggtaatctaa 126841 aagtagggga tgatttcttg ccaataaatt gataatcaaa atcattccat tctgcttcct 126901 tttgttgttg accaaggcgt cgagcttcta gattctcata tgggcctcct ggaagaagtc 126961 ttaaggcttg ttgaataatc cttattgatt ctttcatttc gccaattcgt accaaataac 127021 gagctaatga atcccctcct gtttgccatt ggatttgcca atctaattca tcatagcatt 127081 cgtaatgatc taccttgcgc agatcccatt gaattcctga ggctcgtaac atgggtcccg 127141 ataagcccca atttatagct tcttctctgc taatgaaacc taccccttct acccgtttga 127201 gaaagatagg attatttgta ataagtcgtt catattcatc gatttttgag agacaatgat 127261 gacaaaagtc taagcattta tctacccacc catatgctaa atctacagct actcccccga 127321 tccggaagta attgtgcatc attcgcatac ctgtaacagc ttcaaataaa tcataaatca 127381 tttccctttc tcgaaatatg tagaagaagg gggtttgtgc gccaacatct gccaggaaag 127441 ggccaagcca taacaagtga gaagctattc gactcaattc aagcataata atccgtatgt 127501 aactagctct tttcggtatt tgaatatttg ctaatttctc tggagcattt actgttattg 127561 cttcggtgaa catcgtagct aaataatccc atctcgttac gtaaggaaga tattgtagga 127621 ttgttcggtt ctcggcaatc ttttccattc ctctgtgtag atagcctaat attggttcgc 127681 aatcaacaac attctcacca tctaaagtaa caataagtcg aaggacacca tgcatagatg 127741 ggtgatgggg tcccatactc acaatgactg tatcttttct tgtgagagtc atactcgtaa 127801 ataattcccc ctttgttttc caagaattcc agatccgata aaggattgga atctgaatca 127861 ttcagttagt caataataag aaaataaaaa tatattcttc gatctaattc aacgtttttc 127921 tgatttccga atccctagag ttagaattat gctggtataa gaagttatat tcttttcgga 127981 taaatatctt aagagacgct tgcgtttccc caataatttc cacaaacctc tttgcgaaga 128041 ataatccttt ggatgcaatt ttagatgaga ggttagtttt gagactagat cagtcaaata 128101 agatatttga gattcagtag acccggtatc tttctttcga gaaatcaatg atgaattaat 128161 agacatatgt ttttttatcc taaaataata agaatatttt ctaattttat tttataaaat 128221 attttcaaga atccactcat cgatctgagt aatataagtc tggattaaga acattctcag 128281 aattaggatc aaagatatca ttgaataatt tcaaacccat tgcatctgtt aatggaaatt 128341 ggttcctagc ctctaaaaat gtcatacata gaagatttat ccattgttgt cataaatgat 128401 aatcattaag attgctacga tttagcaatc ttaataaaag ggatcgttga ttctatgctt 128461 ttttttattc tacagaacgg tatacatgcg tatcttgagt atagtaaatc gactcccatt 128521 attggtgttt agatagaatc tatttataca ggctaattct tctaaacgat gactaggcca 128581 aaggaaacgt ttgattattt ggattttact ggaatctttc gattcctcac ccttacttgt 128641 ctcttgtatt cgaaaaggag aatctaagag ataatcttct ttccttttta cgtatttggg 128701 aattcccata gatccgagaa tttgtgattc ccggcccctt ctaggaagta aaagatcgtc 128761 aatattatag agataaggga gatctatcct tccatccata aagagtagcc ggagtttaca 128821 ttcgtcaaag atatctttat tcaatttctt tttaaatcga gttttgaatt tcaatagaat 128881 ggtaactatt ttatacatta gaatctgatc atcaaagatt cgcgaaaaac gatgacccga 128941 aaagatgaat agatcgtcta ggatctcatt atcttcttct ctaaagaaaa gattgactag 129001 atctgggtct atacctatat atgtagtgag atcgtgctta tcttgcaata tgttggtgag 129061 agataagaga tctttaaatc tttttaatct cttatataat gcctcagatt tccacttcca 129121 ttgagatata aaataatgag tcaatctgtc tctgaaaagc atttcatcta atcttacgac 129181 agattcatct tccatgccta gatcaatttg ttcatttaga agagaattga acaaaggact 129241 tttcagaata caagtcttag gcccaaatct tgattttaat cttgtcttag acatcttttg 129301 attgtttata acatctggaa taatccatag ggctaaatcg gtttttgaaa tcaactcgtt 129361 ctctaaatta gagatttgtt gatcaaaagt cttttgtttt tttcttccaa caagggtttt 129421 gatacgattt ttccaccaat ccttttgttt gattccattc ttcaatactt cggatctgtg 129481 tctttggaaa gaattgacaa gattgaagag cagattagta tatttatgaa gtttattaat 129541 attattaatc cggtctctga ataacggatt ttcaatatag aaacagaaaa cattcccatc 129601 tttatcagag acatatttgt cttggttttc aaaagaattc ctttcaatct cttcgaatct 129661 attcagattc tcaacattga ctctccactc ttgtggtgct attttacgcc atacttctaa 129721 tggcaagtta tacctgttga aagatcctaa ccaattttcc caatcattcg tagttagatc 129781 caaaagttgc ttggaaaggc cctgtatcaa aacaaggtct ttaatcggtt catcgatata 129841 ttcttttata atcttgtctc tgttattaag atatcctaag aaatcctgtt ttttttcctt 129901 atggttcttg gctgggagag atatcgagtt tttctctctc tttattgaaa gatcaccatt 129961 ctcaagcaaa tcattatatt tttcataatt ccactcctct ctataatcat tccagtcaga 130021 atctttgtcc aattcttctt gaatactaag cttttttgga attccattat ctttcaaggt 130081 cttcatcaaa tcatttagat ctacattgtc tgttccattt gtataccaga aattgtcata 130141 aatatacgct tgagagaatg atccgatcga tttattatct acttggaaca tattcttgtc 130201 cgtattcgga atagccaggg ccgactcctc tatgtcagca tcattctcga taatattctt 130261 tatgactctt actaattgga attgtaaagt catcaatgta acagaataat gagagaggat 130321 cctattaatt gtttggagag attttgctat tgataaatat ttatcaatga attctgtgat 130381 tcctatacgc aatatcaagc ttcttcttcg aatatctatt agtttctttt taaaccttaa 130441 agaactaata atatttgtat tgtttaatct tttatctatt tccactttac tgagatgaaa 130501 aggtttaatc tgttcatctg tatcaatctt tggaagtaat tgattattag gttgataaat 130561 gggaaatcca gttttcaatt cagatccacc gaaggcatct gatactattt tattgtcaat 130621 accagtaatc aattgagagt aattaaggat atgaatagat tctttatcaa tcttcccact 130681 atttattttt actctcagtt tagtctcatt gttttcaatt cgacgaggat cgtcttgaat 130741 caccctgaat ctgctgtcca aaggattcaa acctttccgt acgcttgaac cgaatttcat 130801 aggtatacct aatctagaac aaaaagaacc tatttcccta gttcgcaatg aaatattatt 130861 cctccaggtt tttattaatt ctcttttaat aggtttccaa aaagaaggct ctttcttaat 130921 agtgccgaaa ggtctatctg tctgaaatcc ccagattgtc aaataagtag cttgaaaccc 130981 ttctttctct gacttatcct ttcttcttaa ttttcgtact ggaatatcct tgaggtttat 131041 cttacttccc cgaggaattg atggtttctt cttatgatta tgccaaggct tcaatcgaaa 131101 aggatgaaca atctttatct gaaggccttc tttcaaccaa cgagccggca aatcagtatc 131161 tgaaaactct tcaccgtcat aagtgcaatt aatatgagat tctttgtggc actcactcca 131221 atcctcattc cattccggaa cttggaaaag aaagatacga acaaaattct tcaatgtgat 131281 taatataggc agtttcacat attttcgaag attcgattgt agcactaata atagactcct 131341 tcccgtgtga atagaactta catctaatct agcggatatc aacgagcgta ttacttgtcc 131401 attctttaag gatgaagacg ctgatagttg ttgtttaaat tctctattag tctcctgttc 131461 tgaattgatt gtcaatcttt cagaatcgat aaaacctttt accgagggtt gggataaggt 131521 aggtatttct agtaatctta agaaaaatgg tgaatgtgct ttttcttgga acaatttcca 131581 aactagcacc ttacgtctcc tagaacgcat agaccccttt ataagttctc gtcgaaaatc 131641 aggggtttct gagaaacgtt tgattatcct tttcggtttt gatatcgttt tttcgatact 131701 gattcctaga ttctttaatt tcctattacg aatatcagta actccaccca caatatcaaa 131761 tggattatca agtattaatt gactattatc agttaattct ttcatcattt cttcaatctt 131821 atagactgag tgtactctct gttttgagta agaatatctt ttcttgtcaa tcgaagagat 131881 atctaataga tcgatccaat cgaagccatt acaatcttct tgaaggcaga taaagaatag 131941 agtgcgttct ggcagcggaa gcttaaatac ttgctcccaa gttacttccc cggatggtaa 132001 atcttcctcg ttatagagaa gatccatttc atcaaatatc tgttgaatcc taacgtcttt 132061 cgaatcctcg aacatgaaca agaatatatc ttgagctctt ggcggtattg tttcccaagg 132121 aagaggaatc ttatcacagc ttaactgtcg gtacctatta gaaatccaat cttcaatctt 132181 ggcataatca ttatattgtt cagtatattt gttattatta tctattattt gattatctat 132241 tcttgatttt aacaaatcgt acaataccca aagtgatttt gagattggaa actttatccg 132301 atataagtta cttagaaagg ggttagatgc cttaggtaat ctttcttgat ttaggtgaac 132361 caattccgtc tttctctcta ttatctttga aaagatgcaa ttagtatcta aagatcgaat 132421 tcgatctttt aattcattga ttagagcttc ttttctgatt gattggtctg agatccaata 132481 tttatacgga tttacattga attctttctc tttaaaaggt tttagtagtt gtaattgatt 132541 ctcgaagatt gataaactgg gtaatgctgt aagagttatc ctttctttcc catcactcaa 132601 acatttatca aagaaatgag ttgatacttg tcttttcaca ggactattac cactgaacca 132661 acttgttatg tatcgtatag gccgatttgg tctgtgttga tcaaaataag aagtaggcca 132721 ttgtttgaac atccataata tccagtctgg taattcataa aaattttctt caagcaaaac 132781 cagtctatcc tcaattttat aagtaaaaag agataccgga gctttgccta aatacagcaa 132841 tacgtttata taaataataa tactaaaagt attgtggata atccgtttta tcagtacata 132901 gagaatcggt gaatcactct gtaagcgaac tactaataat ctcaatagat taataaatag 132961 tatatgaccg attaaccaac ccataaaggt actaattaga aatatcacat tagtactgta 133021 acggaaaagg aaaagatgaa taagtctagc taatacagga cttggcaaaa gtattggatt 133081 cagaatctgt agaagaaagc tttctaagaa tatgttatag atccgagaat caattaataa 133141 accaatagga tgaaagactc gataatctga taaatccttg gttcgaaacc agtagaacaa 133201 catgtacggc actattaaga gtgttataaa atgaggtttt attatcaata tgtataaggg 133261 tgaatagaat attgataaaa atagtattag ctgacctatt atcaaaccac tcaaagctgc 133321 aaagccacta gcatttcctc tcagtagaaa agatcttata gatatgatat gggaaggccc 133381 aataggtaat gttgatagta acccataata gagtccaaag agtatacaag gacttgctac 133441 tcttatccaa gttaatatta aggcttggaa tagatacaac gacagttgaa cgctttctac 133501 gttcataata agtattttcc tttgtttctg ttatcttttt ctttcgatgt atgagcaata 133561 tttattgatt agttctatag tataccactc tttttggaga agatcaatag atttattaac 133621 cataaaggat atcctttatg gttaataaat ctaagataca tttcgagata tttccatcaa 133681 tcagttatca attcttggta ttttctttag tcttaatcag attgttagtg cttgttttga 133741 aaggttgttc caacccaagc gtcctaaatc agtattccgt cgtagaggac gcgtaacaag 133801 ttcaagcaca tcttttgcgt taataaatcc atgaatctga gcaaatgtaa attcgacaga 133861 ccattttgta tcgatacctc ttgcttctaa aggattggcg tgagccatcc cagtgattgc 133921 taaatcaggt cttaattcat gcatacgttg catttggtta taattatcag gtttctcaac 133981 gattcgtggc ataggtgtat tcattcttat gcaagtatct cgtaaaagag ataattcagc 134041 agcttgatat cgtttgtcca gataaggaat acctatttcg taaacgatca ttccgcatcg 134101 aattagaaat cttgctatcg agatttctaa tagattatct cccatgaaga atatagatct 134161 tccagttact attccaagat aatctttcaa gcctttccat atcttatttt ctctttcttc 134221 taagccataa ggttctatat taaagacaga acaaatcttc tcgatccaag cacgtgtccc 134281 atctggacca atcgggaaag gtgctccaat tagctggcat ttcctacgcc gcataagagt 134341 tgttgctgta cggcttagaa aaggatttac accacaaaca tagactcctt ttcctaatga 134401 tggaagttct atatatctct gagacggtaa ccaacctgag actcgaatgg attggcgttc 134461 taactctgaa tttaattgag aagctacggt tgagggtaga gatccgaaaa ggactaggat 134521 acaattattt tgttcctgct tatttggaag agatcttgtt gattttacca gagtgatatt 134581 tgattcatcg atatcttcaa ttgaattttg attctgtttg tatctttgcg cattatattc 134641 cgggcatcga tgtgtcatag cagcgagtac agtatcttct ccttgagtaa aggcataatc 134701 taatccattg gctcgggcta ctataatcgg tattccaagt tccttttcta gcttcggtcc 134761 tataccttcc agatccattt ttattatttc tgtagtacac gtaccaatcc aaataataac 134821 gctagggtct ctatctctct ttatttgacg acagattctt cctaattctt cttgatcatt 134881 caattatgct gagatatccc cctcttctag ttctgccatt gcataacgag gttccgcaaa 134941 gatcgtaact ccaagagcat tctgtaaaaa ataaccacaa gtctttgttc ctaccactaa 135001 gaaaaaacta tcttctattt tttgatacaa ccaggctaca cagctaatag gacaaaaagt 135061 gtgataatta ccagtttcac attcaaaagt gagagtttca agagtcttca tgaaagagtt 135121 tcctaaagaa gtatgaatgg aaggaaggat cagatcttta atattagatc atcgtagaat 135181 caagcagaac gtcctgttca ttttgggtct tcttattccg ttgtccatga tcaggattta 135241 gataaaaatc tgagagtaga ctgaataatt ccctgtctgg aacctctcta gggacaattc 135301 cttctggttg agataatagt tgatctgcta tgttcaaata gaaatcacaa acataattaa 135361 gatttgattg atattcagcc atttcaaaca gggttttccc ttttactcta gagacccgaa 135421 tgtcttcgat tagaggtaat acttctaata caggcattgg acaagcttct acatacttat 135481 caatcaaatc tctcttagct gttcggtttc ccactaaccc tgctagccgt aatggatgag 135541 tatgggcttt ttctctaacc gatgcagcga tacgatttgc tgcaaaaagg gcatcaaatc 135601 cattatctgt tataatgata caataatcag cataatttaa tggagctgca aaacctccac 135661 atactacatc tcccaaaaca tcaaagagga taatatcata ctcataaaaa gcattcaatt 135721 cttttaataa cttaactgtt tctcctacta cataccctcc acaaccagcc cctgccgggg 135781 gaccgcctgc ttctacacaa tctactcctc cataaccctt ataaacaaca tcttcaggcc 135841 acacatcttc ataatgataa tctttcgatt gtagagtatc tataatggta ggtattaaga 135901 atcctgtaag agtaaaagta ctatcatgtt tagggtcaca tccaatttgt aaaactcgtt 135961 ttccgcgtct cgctaaagct atggatatat tacaactagt tgttgatttt ccaatcccgc 136021 cctttccgta gactgctatc ttcatttcat aaaactccaa ttcattcttg attatcctga 136081 ctgttcttcg tttcccttat ctttgtagtt atacggggtt acattgatta ttattatgat 136141 agattcttca taagaggtat ccgttctgtg agcaagctta tttcaatctt tgacaaatgg 136201 gtaattgacg gaatgattaa tggtatcggt atctcgagtt tcttaggggg agagagtgcc 136261 agatacgggg aaggcgggag agtatcctct tatttattcg gattaatgat tgggatcgtt 136321 ccattgttaa ttgttttcgt ttctctcgtt tcctaagagt ccttgcaaga gaagatattg 136381 cttctacttc tagaagtaga agaatcctgt gactattcta ctatgtttct cggaaaagga 136441 gaaatagaaa ctagtgattg ttgattgatc gatatgcatc ttaggggggg gggggctggt 136501 gcgtaagatc ttagtcatga ttgatcgccc cccccccgaa tcaggggctc tatccgagga 136561 ggcagagggg attgctatca ctaccgcttc gtcctataat aagagttagg gtgtaggcga 136621 ggtttatgaa gtcccgaagg aaactttgtg tcttcgaata atgaacgtct tagaggttaa 136681 gggttaaaag gcggctcaag aaacaagagt ctttgaatgt atatgggctc tgcaatcttc 136741 ctcggtagct cagtggtaga gcggtcggct gttaaccgat tggtcgtagg ttcaaaccct 136801 acccggggag attcatctat ttccagtgga atagaggata gtttgaatca tgtagatatt 136861 gatgcttctc ttcagtagga taataataat acttcattca tatacgccaa agaaggggaa 136921 ggtactttct catgatatag aattaagaat gggtaatcaa tatcctccct tccctattag 136981 agaatccaat cgaatgcttt taagggcagg acccgagagt ttaacgttgc ttcttcgaag 137041 atctccgctt aagagaaaca aatcaatacg agagagtcgg gtcatatccg gcgaagaaag 137101 agctatgcct ttgtggagga tcaagtccca tggttttctt tggctttgca tctttgcgcc 137161 tggaatccta atcatactat ttgcttgcct attgactagt gagagataag ggacgtttag 137221 ggatggatga ttttgggcga ggagggattc gaacccccga caccgtggtt cgtagccacg 137281 tgctcttatc ccctgagcta caggccccgt ctccactgga tctgttccgg tggaactttt 137341 ctagaataga ctagaatgga ggcaaccttt cctctctcca tccattccga gttagagatc 137401 ttaggatcct cttctccccc aacccagcca gaaagagagg ggattctatt ctcatgaagg 137461 gagaaagacg gaggtgctac tagaatatcc atggggtctc gaacggagaa ggagatgacc 137521 tttaagggag aatggaaggg attgagccct ttctctcatc ctggcgtcga gctattttcc 137581 cgcagggctc cccctgcagt atcgttaccg cggtagagtt tcaccaccaa gttcgggatg 137641 gattggtgtg gttcctccac gcctaagaca ccagaatatc aacctgtgaa cgaaaaggaa 137701 aagaaggaag aagtgtatct atgtatctat tggttagttc ttagaaggct ctaattccga 137761 aaggtacacc agagggaata gtgtaccttc caggcagaga acattattat gacccaggtt 137821 tccatccctt cgatggacgg agaaggggcg gaactcttgg atcgacttct atcctaggct 137881 gatgctgatg ttgtcagaca atcgaataat aggtggcatt gcctaatcga attgatcagg 137941 ttatgcgaga acaaggttca aatctctcgg tctattagga tgcctcagct gcatacatta 138001 ctgcacttcc acttggcacc tatcgtaatg ataaacggct cgtcttgccg tgaccttatc 138061 ttggattctc agatcaaaga cttccgtcgt ttcacagttt gccaggacgg gtaatccgtc 138121 gttgctccgg ctctttctgc gtccagagtc ttccgagtga gtaagaataa gagagcactc 138181 atcctgaggt gggcttccta cttagatgct ttcagcagtt atccgctccg cacttggcta 138241 cccagcgttt accgtgggca cgataactgg tacaccagag gtgcgtcctt cccggtcctc 138301 tcgtactagg gaaagatcct ctcaatgctc taacgcccgc accggatatg gaccgaactg 138361 tctcacgacg ttctgaaccc agctcacgta ccgctttaat gggcgaacag cccaaccctt 138421 gggacgtact accgccccag gtggcgaaga gccgacatcg aggtgccaaa ccttcccgtc 138481 gatgtgaact cttggggaag atcagcctgt tatccctaga gtaactttta tccgttgagc 138541 gacggccctt ccacttggca ccgtcggatc actaaggccg actttcgtcc ctgctcgact 138601 ggtaggtctt gcagtcaagc tcccttctgc ctttgcactc gaaggctgat ttccgtccag 138661 cctgaggaaa cctttgcacg cctccgttac cttttgggag gcctacgccc catagaaact 138721 gtctacctga gactgtccct tggcccgtaa gtcttgacac aaggttagaa ttctagctct 138781 tctagagtgg tatctcactg atggctcagg cccccccgga aaggagcctt ctttgccttc 138841 cacctaagct gcgcaagagg agcccaaagc caatcccaag gaacagtgaa gcttcatagg 138901 gtctttctgt ccaggtgtgg gtagtccgca tcttcacaga catgtctatt tcaccgagtc 138961 tctctccgag acagtgccca gatcgttacg cctttcgtgc gggtcggaac ttacccgaca 139021 aggaatttcg ctaccttagg accgttatag ttacggccgc cgttcaccgg ggcttcggtc 139081 gccggcttcc ctgtcatcag atcaccgact tccttaacct tccggcactg ggcaggcgtc 139141 agcccccata catggtctta cgactttgcg gagacctgtg tttttggtaa acagtcgcct 139201 gggcctggtc actgcgacct cctttgtttg taaggaggca ccccttctcc cgaagttacg 139261 gggccatttt gccgagttcc ttagagagag ttatctcgcg cccctaggta ttctctacct 139321 acctacctgt gtcggtttcg ggtacaggta cccacttgtc gaaggtcgtt cgagcttttc 139381 ttgggagtgt ggcgtgggtt acttcagcgc cgtagcgcct agtgctcgga cattggctcg 139441 aggcattttc tctacctctt attccctttt acgggtccat taaccttgcg tccttaaacc 139501 gatcaccatc cttcggctaa cctagcctcc tccgtccctc gggaccaaca aggggtagta 139561 caggaatatt cacccgttgt ccatcgacta cgcctctcgg cctgatctta ggccctgact 139621 caccctccgt ggacgaacct tgcggaggaa cccttgggtt ttcggggcat tggattctca 139681 ccaatgtttg cgttactcaa gccgacattc tcgcttccgc ttcgtccaca cctgcttgcg 139741 ctgatgcttc tctctaggac ggaacgctcc cctaccgatg tattttgaca tctcacagct 139801 tcggcagacc gcttagtccc gttcatcttc ggcgcaagag cgcttgatca gtgagctatt 139861 acgcactctt tcaagggtgg ctgcttctag gcaaacctcc tggctgtctt tgcactctta 139921 cctcctttac cactgagcgg tcatttaggg gccttagctg gtgatctggg ctgttcccct 139981 ctcgacgatg aagcttatcc cccatcgtct caccggttga ccctttctcc ctccttattt 140041 ggaggtcata tctagtattc agagtttgcc tcgatttggt accgctctcg cagcccgtac 140101 cgaaacagtg ctttacccct agatagtcca gtcaactgct gcgcctcaac gcatttcggg 140161 gagaaccagc tagctctggg ttcgagtggc atttcacccc taaccacaac tcatccgctg 140221 atttttcaac atcagtcggt tcggacctcc acttagcttc acccaagctt catcctggtc 140281 atggatagat cacccaggtt cgggtccata agcagtgaca attgccctag gaagactcgc 140341 tttcgctacg gctccggtgg gttcccttaa ccaggccact gcctataagt cgccggctca 140401 ttcttcaaca ggcacgcggt cagagatcca attctcctcc cactgcttgg gagctcacgg 140461 tttcatgttc tatttcactc cccgatgggg gttcttttca cctttccctc acggtactgt 140521 tcgctatcgg tcacccagga gtatttagcc ttgcaaggtg gtccttgctg attcacacgg 140581 gatttcacgt gccccatgct actcgggttg gagcgtaagc cagtggtact tccggctact 140641 ggactctcac catctggggt gcagtactca accgcttcgc ctagcaacac aggactctca 140701 tagctctccc acaaccccgt tctcacggtt taggctactc ccatttcact cgccgctact 140761 atgggaatcg cttttgcttt cccttcctcc ggctactaag atgtttcagt tcgccgggtt 140821 gtctcttgtc tgcctatgaa ttcagcagaa gttcaaaagg ttaccctatt cgggaatctc 140881 cggctcaatg ctcattttca gctccccgaa gcatttcgtc gtttactacg cccttcctcg 140941 tctctgggtg cctaggtatc caccgtaagc cttttctcgt ttgaacctcg tcgttgaata 141001 ctatgcttgc catcttaagg tgctaaatac agagatccta tcaacgtcca tagagaatag 141061 attctagatc aacctcccca cagcacaggg gatcaagatt attccatact aaatactaat 141121 atatgactaa tgtatgcgat tctattaact cggatcacag gctggagata agcggattcg 141181 aaccgctgac atccgccaca gggtaagcta ccatctctca ggcctacctg attgattcta 141241 ccatggaggc cggcaataga caatggctcc cccccgaaca cggcctacaa ctttcatcgt 141301 accgtgctct ccgaggagca actcttctcg aattgaaagg cgccaggttg agatcccatt 141361 ctagggattc ttctagctag atactcccga gctacaggag aaccaaaagc taaggttctt 141421 gccctttccc ttctttgccc gactctttag tctcaataat gagtagttgc ccctctccga 141481 ccctctccgc caaccggagc ggacagctaa agcgttccac ttctcggatc gggttatatg 141541 gtcggtctgt gacccccgga tgccttcagc atccttgggg cgatctcgta gttcctacgg 141601 ggtgggggcg atggggtcgg tccatggatt ctagttcctt ctgccacact gtgctcaggg 141661 agttgaagag agatagtcta tcaggctgtt cgcaagggcc agctcgaccc tcttccccag 141721 agatccagat gaggtaaccc tgggagagcc gccgactcca acaaccgtcc atgtacgatc 141781 catactggat ctaaccaacc acctatccta cccccctaca ttctcgacag ctcatctttg 141841 tcccagtaga gtctatcagt ggcatgttct ggtcctcctc cccattcctg aggaagagtt 141901 agccaccggt tcgggtaaaa gatactatca ctaccgccta gatgattagg cacccaaccc 141961 gtaagcgcaa cgacccactt gcaagggcgg cgctctacca actgagctat atcccctgtg 142021 cccgggccaa gtgaagcata caggaatgga tcagatgctt cttctaattc cccttagcgc 142081 agctgggcca tcctggactt gaaccagaga cctcgcccgt gaagtaaatc atcgcaccta 142141 caccactatc ccataagaat agagaatcaa gagattcttt cttaagagcg attcatcctt 142201 cccgaacgca gcatacaact ctctgtcgta ctgcgctctc caagtgtgct tgtttcccct 142261 tcctctcttc tttgccaaca gcgagtttat gctctggaaa gggacttcaa tgggagagaa 142321 aaggaggcgt taagagaccc tccctcctag ctcaaccctg gacactctag gatccttctt 142381 cgaacctgct gctcctatca ttgaaggatg aataaagaca tcccattcca tagatcagat 142441 ctggaggctg tttggaatac ctgatagggc cgagggaccc gggagtgatt tgcatcagga 142501 atcgctccta tgtctaggcc cgatctcctg gcccctccag ggattgtttc tgaaacttgt 142561 cccccctttc tagattcctt acctccagag aggttctatt gattgctact tctacggacc 142621 tttatagaga gcagaatagg ctcggatagt atgatccctc cgtcacccta gaatgagaag 142681 ggtgattccg tagttcctgg tccgtgaata tgcgtcatca ggtgctccgt ttctcacctc 142741 taggctgaac ttcagcctgt gctcgagaga tagccaccca tacactgata aggagtgtat 142801 agattcccga gaagagggga gccatcatcg agaattggtc ctccctccct ggatcgcccg 142861 gatcccacga gtgaatagcg ggttgaatca tcatcagatc tcacctgaat cgcctcatct 142921 atcttcctga agagtaattc gttctcacga cctagttgag acaggaagag tacgccatgc 142981 taacgttcct tggatgatct ccatcttcgg gtcaggcgca gatgagcaca gtaaactacc 143041 catgtgtctg aaagctttca cagcccaagc acaacgacgc gattatcagg ggcgcgctct 143101 accaactgag ctaatagccc gtcatgcagt cctccctatc cctattagga ataggagggg 143161 cctacaatac cggaggcgag agggaactta tccttatccg tcatccctcc ctaaggaagg 143221 atggggatgg gatcactctc agcttattcc gacctgagag aataaaacaa gctcatggac 143281 ttggtcttgc ttcactgtca agacaagata accccaccca taagtggatt ctgtggatca 143341 cttctctcgg agtttcggag tgcgaaacag tggatagaag accaaagtac caaataagta 143401 ttaactctcc ctgaaaagga ggtgatccag ccgcaccttc cagtacggct accttgttac 143461 gacttcactc cagtcactag ccctgccttc ggcatccccc tccctacggt taaggtaacg 143521 acttcgggca tggccagctc ccatagtgtg acgggcggtg tgtacaaggc ccgggaacgg 143581 attcaccgcc gtatggctga ccggcgatta ctagcgattc cggcttcatg caggcgagtt 143641 gcagcctgca atccgaactg aggccgagtt tctgaggtta gcttgccctc gcggggtcgc 143701 gaccctttgt cccggccatt gtagcacgtg tgtcgcccag ggcgtaaggg gcatgctgac 143761 ttgacgtcat ccccaccttc ctccggctta tcaccggcag tctgtttagg gttccaaact 143821 cattaatggc aactaaacat gagggttgcg ctcgttgcgg gacttaaccc aacaccttac 143881 ggcacgagct gacgacagcc atgcaccacc tgtgtccgcg ttcccgaagg cactcctctc 143941 tttcaaaagg attcgcagca tgtcaagccc tggtaaggtt cttcgcgttg catcgaatta 144001 aaccacatgc tccaccgctt gtgcgggccc ccgtcaattc ctttgagttt cattcttgcg 144061 aacgtactcc ccaggcggga tacttaacgc gttagctaca gcactgcacg ggtcgatacg 144121 cacagcgctt agtatccatc gtttacggct aggactactg gggtatctaa tcccattcgc 144181 tcccctagct ttcgtctctg agtgtcagtg tcggcccagc agagtgcctt cgcctttggt 144241 gttctttccg atctctacgc atttcaccgc tccaccggaa attccctctg cccctaccgt 144301 actccagctc aatagtttcc accgcctgtc cagagttgag ccctgggatt tgacagcgga 144361 cttaaagagc cacctacaga cgctttacgc ccaatcattc cggataacgc ttgcatcctc 144421 tgtcttaccg cggctgctgg cacagggtta gccgatgctt attcctcgga taccgtcatt 144481 gcttcttctc cgagaaaagg agtttacgac ccgtgggcct tctgcctcca cgcggcattg 144541 ctccgtcagg ctttcgccca ttgcggaaga ttccccactg ctgcctcccg taggagtctg 144601 ggccgtgtct cagtcccagt gtggctgatc atcctctcag accagctact gatcgtcgcc 144661 ttggtaggcc attgccccac caactagcta atcagacgca agcccctcct tgggcggatc 144721 cctccctttt gctcctcagc ctatggggta ttagcagtcg tttccagctg ttgtccccct 144781 cccaagggca ggttcttacg cgttactcac ccgtccgcca ctggaaacac cacgaggatg 144841 cttcccgttc gacttgcatg tgttaggcat gccgccagcg ttcatcctga gccaggatca 144901 aactctccat aagattcata gttgcatcac ttatagcttc ccccttcatg aatgaagctg 144961 atttcagatt gtcttcttat cttcaggtag aacttaggtt tcttcacttc atattagctt 145021 gaagttcact cctagtatac ccacctctac cactccgtgt caatcccaca agcctcttct 145081 cctgccctca gaccctaccc ggatccctgt acgaatcgta cgtagagtat gctgagagga 145141 acggtatcaa gcctatcacg tactaccggc ttggagacgt tttagacgat ctagtccgat 145201 ctcaaggtta cagagacatt gttacaaaga ggagagccaa cggaagggta gtcattggcg 145261 gaggggggga gcccttaagg aagaacatca gatccgacgt agttgattat atgaccaaga 145321 cagacccctg gccgggattt gacaaggata gagagggtta cgacgagcac gatagtgtca 145381 atagtggcga tcttttacgt aaattacgaa atttaccaaa atgacggaaa taatcgacac 145441 tctaagtgct ctccatactt tgcttttttt gaagccttct tccgttttca aaccagagaa 145501 aatcgttgct atctatcccg ttgtatctat acgtgttgta gagagagaag tatctatggg 145561 acatatcaga gagtgagtat ctctgcagcg cttagggata atcaggatcg aactgatgac 145621 ttgcaccacg tcaaggtgac actctaccgc tgagttatat ccctactctc tttgctactc 145681 ttctatcaaa tgatcgctta cccatgagga gagtgggaac taaccaactt gaatggtcat 145741 ggtcaaagaa ctagattcta ctagcttagt atgtatctct tgagtaatct gggactggaa 145801 cgttttccct tccacacaca cactctatta gtacttctta gtacttatcc cgaaacaaag 145861 aagtgagaaa tccgagattc tttctgttat aacccgagga aatcctattg agaataggaa 145921 ttgtcaacga ttcggtccat aggaaatgac tctccagacc agaaggcgtt attctatctc 145981 ccaatccaat tctgacgagt catactcgag gaagatgttt ctcccataca gataagaaac 146041 agagctcttc tttctctagc atcagagata ttacctatgg gttctctatc ttaggcttag 146101 ggagatgaaa gtttagccgt gcacctagtc tagttgttaa ccctattcaa tccaaaaaga 146161 aacgaaatcg ggaagtctcc aatgcgagag atttctgaga gacatgttct gatagaatcg 146221 aagctacctt cacccaacgt atcagtaacc ctatccaacc tataactata tcagattagt 146281 acattggcac cctaccgaaa cgggatatac aactgacatt gctaaaccca tctgcaatta 146341 gcactagatt agcattactc tactacatag tatagatgaa atactcgtcc tcgtcatttc 146401 tttggatcaa cgttggaata caaccagatt cgcggaatct gtgtcatgca ttggtagtaa 146461 caacagcttg acccctttga gctatcgtgg tataatattc ttcccacaga acccgagatt 146521 cttgtccacg aaaagggaac gagatggttg acttgtgcca atgggttatc acagaatggg 146581 ctagctaagc caaaatcatc gagataacag agtcctcttc ttccttctct gtctatatcc 146641 ctggttttct tgactatttc catttcattc tcatggctat tagcaggcct tacgcagtca 146701 tcagatctct atctgatata gatagatatc attataggtt actgaacccc tttcagatag 146761 cctcgtcgca acgaacgaaa gaatgaaagc caaatcgaat caaagatctc gttagaatgg 146821 attctctttt gaatggtgat taataacatg gataagctta tactaacccg tcaa

Figure S2. Full plastome sequence of V. speciosa . The five 27-bp tandem repeats comprised in positions 44286-44420 are labeled.