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Shedding Light on Microbial Dark Matter with a Universal Language
bioRxiv preprint doi: https://doi.org/10.1101/2020.12.23.424215; this version posted December 26, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 1 Shedding Light on Microbial Dark Matter with A 2 Universal Language of Life 1,* 2 3 2,* 3 Hoarfrost A , Aptekmann, A , Farfañuk, G , Bromberg Y 4 1 Department of Marine and Coastal Sciences, Rutgers University, 71 Dudley Road, New Brunswick, NJ 08873, USA 5 2 Department of Biochemistry and Microbiology, 76 Lipman Dr, Rutgers University, New Brunswick, NJ 08901, USA 6 3 Department of Biological Chemistry, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina 7 8 * Corresponding author: [email protected]; [email protected] 9 Running Title: LookingGlass: a universal language of life 10 11 Abstract 12 The majority of microbial genomes have yet to be cultured, and most proteins 13 predicted from microbial genomes or sequenced from the environment cannot be 14 functionally annotated. As a result, current computational approaches to describe 15 microbial systems rely on incomplete reference databases that cannot adequately 16 capture the full functional diversity of the microbial tree of life, limiting our ability to 17 model high-level features of biological sequences. The scientific community needs a 18 means to capture the functionally and evolutionarily relevant features underlying 19 biology, independent of our incomplete reference databases. Such a model can form 20 the basis for transfer learning tasks, enabling downstream applications in 21 environmental microbiology, medicine, and bioengineering. -
Actinobacterial Rare Biospheres and Dark Matter Revealed in Habitats of the Chilean Atacama Desert
Idris H, Goodfellow M, Sanderson R, Asenjo JA, Bull AT. Actinobacterial Rare Biospheres and Dark Matter Revealed in Habitats of the Chilean Atacama Desert. Scientific Reports 2017, 7(1), 8373. Copyright: © The Author(s) 2017. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. DOI link to article: https://doi.org/10.1038/s41598-017-08937-4 Date deposited: 18/10/2017 This work is licensed under a Creative Commons Attribution 4.0 International License Newcastle University ePrints - eprint.ncl.ac.uk www.nature.com/scientificreports OPEN Actinobacterial Rare Biospheres and Dark Matter Revealed in Habitats of the Chilean Atacama Received: 13 April 2017 Accepted: 4 July 2017 Desert Published: xx xx xxxx Hamidah Idris1, Michael Goodfellow1, Roy Sanderson1, Juan A. Asenjo2 & Alan T. Bull3 The Atacama Desert is the most extreme non-polar biome on Earth, the core region of which is considered to represent the dry limit for life and to be an analogue for Martian soils. -
Insights Into the Codon Usage Bias of 13 Severe Acute
bioRxiv preprint doi: https://doi.org/10.1101/2020.04.01.019463; this version posted April 4, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Insights into The Codon Usage Bias of 13 Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Isolates from Different Geo- locations Ali Mostafa Anwar *1, Saif M. Khodary 1 1 Department of Genetics, Faculty of Agriculture, Cairo University, Giza, 12613, Egypt *Correspondence: [email protected] Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of Coronavirus disease 2019 (COVID-19) which is an infectious disease that spread throughout the world and was declared as a pandemic by the World Health Organization (WHO). In the present study, we analyzed genome-wide codon usage patterns in 13 SARS-CoV-2 isolates from different geo-locations (countries) by utilizing different CUB measurements. Nucleotide and di-nucleotide compositions displayed bias toward A/U content in all codon positions and CpU-ended codons preference, respectively. Relative Synonymous Codon Usage (RSCU) analysis revealed 8 common putative preferred codons among all the 13 isolates. Interestingly, all of the latter codons are A/U-ended (U-ended: 7, A-ended: 1). Cluster analysis (based on RSCU values) was performed and showed comparable results to the phylogenetic analysis (based on their whole genome sequences) indicating that the CUB pattern may reflect the evolutionary relationship between the tested isolates. -
Codon Usage and Adenovirus Fitness: Implications for Vaccine Development
fmicb-12-633946 February 8, 2021 Time: 11:48 # 1 REVIEW published: 10 February 2021 doi: 10.3389/fmicb.2021.633946 Codon Usage and Adenovirus Fitness: Implications for Vaccine Development Judit Giménez-Roig1, Estela Núñez-Manchón1, Ramon Alemany2, Eneko Villanueva3 and Cristina Fillat1,4,5* 1 Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain, 2 Procure Program, Institut Català d’Oncologia- Oncobell Program, IDIBELL, L’Hospitalet de Llobregat, Barcelona, Spain, 3 Cambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom, 4 Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Barcelona, Spain, 5 Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona (UB), Barcelona, Spain Vaccination is the most effective method to date to prevent viral diseases. It intends to mimic a naturally occurring infection while avoiding the disease, exposing our bodies to viral antigens to trigger an immune response that will protect us from future infections. Among different strategies for vaccine development, recombinant vaccines are one of the most efficient ones. Recombinant vaccines use safe viral vectors as vehicles and incorporate a transgenic antigen of the pathogen against which we intend to Edited by: Rosa Maria Pintó, generate an immune response. These vaccines can be based on replication-deficient University of Barcelona, Spain viruses or replication-competent viruses. While the most effective strategy involves Reviewed by: replication-competent viruses, they must be attenuated to prevent any health hazard Kai Li, Harbin Veterinary Research Institute, while guaranteeing a strong humoral and cellular immune response. Several attenuation Chinese Academy of Agricultural strategies for adenoviral-based vaccine development have been contemplated over Sciences, China time. -
UC Berkeley Electronic Theses and Dissertations
UC Berkeley UC Berkeley Electronic Theses and Dissertations Title Interactions of Microbes in Communities Permalink https://escholarship.org/uc/item/2hg5h7k6 Author Sczesnak, Andrew Publication Date 2018 Peer reviewed|Thesis/dissertation eScholarship.org Powered by the California Digital Library University of California Interactions of Microbes in Communities By Andrew Sczesnak A dissertation submitted in partial satisfaction of the requirements for the degree of Joint Doctor of Philosophy with University of California, San Francisco in Bioengineering in the Graduate Division of the University of California, Berkeley Committee in charge: Professor Adam P. Arkin, Chair Professor Matthew Traxler Professor Michael Fischbach Fall 2018 Abstract Interactions of Microbes in Communities By Andrew Sczesnak Joint Doctor of Philosophy with University of California, San Francisco in Bioengineering University of California, Berkeley Professor Adam P. Arkin, Chair Groups of microorganisms sharing an environment (microbial communities) are ubiquitous in nature. Microbial communities provide essential ecosystem services to other life on Earth by e.g., participating in global biogeochemical processes or interacting with a host’s immune system. Such microbes compete for scarce resources, modify an environment for their own purposes, actively war, and occasionally cooperate. Though numerous studies have surveyed the diversity of microbial life in different environments, few have determined the ways in which members of microbial communities interact with one another. Understanding the ways and means by which microbes interact is essential if we are to understand how microbial communities form, persist, and change over time. Knowledge of these processes will allow us to rationally design microbial communities to perform useful functions and predict how our actions might shift the balance of microbes in a community, and thus affect its function. -
Codon Clusters with Biased Synonymous Codon Usage Represent
bioRxiv preprint doi: https://doi.org/10.1101/530345; this version posted January 25, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Codon clusters with biased synonymous codon usage represent 2 hidden functional domains in protein-coding DNA sequences 3 4 Authors 5 Zhen Peng1*, Yehuda Ben-Shahar1 6 7 Affiliations 8 1Department of Biology, Washington University in St. Louis, MO 63130, USA. 9 *Correspondence to: Zhen Peng ([email protected]). 10 11 Outline 12 1. Abstract 13 2. Introduction 14 3. Results 15 3.1. Identifying putatively functional codon clusters (PFCCs) 16 3.2. Codon usage patterns of PFCCs are diverse 17 3.3. PFCC distribution is not restricted to specific regions of protein-coding sequences 18 3.4. Specific protein functional classes are overrepresented in genes carrying PFCCs while most PFCCs 19 are not associated with known protein domains 20 3.5. Voltage-gated sodium channels include a conserved rare-codon cluster associated with the 21 inactivation gate 22 4. Discussion 23 5. Materials and Methods 24 5.1. Reference genome and data pre-procession Page 1 of 28 bioRxiv preprint doi: https://doi.org/10.1101/530345; this version posted January 25, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 25 5.2. Identifying PFCCs 26 5.3. Calculating TCAI 27 5.4. K-mean clustering of PFCCs 28 5.5. -
Aquatic Microbial Ecology 79:1
Vol. 79: 1–12, 2017 AQUATIC MICROBIAL ECOLOGY Published online March 28 https://doi.org/10.3354/ame01811 Aquat Microb Ecol Contribution to AME Special 6 ‘SAME 14: progress and perspectives in aquatic microbial ecology’ OPENPEN ACCESSCCESS REVIEW Exploring the oceanic microeukaryotic interactome with metaomics approaches Anders K. Krabberød1, Marit F. M. Bjorbækmo1, Kamran Shalchian-Tabrizi1, Ramiro Logares2,1,* 1University of Oslo, Department of Biosciences, Section for Genetics and Evolutionary Biology (Evogene), Blindernv. 31, 0316 Oslo, Norway 2Institute of Marine Sciences (ICM), CSIC, Passeig Marítim de la Barceloneta, Barcelona, Spain ABSTRACT: Biological communities are systems composed of many interacting parts (species, populations or single cells) that in combination constitute the functional basis of the biosphere. Animal and plant ecologists have advanced substantially our understanding of ecological inter- actions. In contrast, our knowledge of ecological interaction in microbes is still rudimentary. This represents a major knowledge gap, as microbes are key players in almost all ecosystems, particu- larly in the oceans. Several studies still pool together widely different marine microbes into broad functional categories (e.g. grazers) and therefore overlook fine-grained species/population-spe- cific interactions. Increasing our understanding of ecological interactions is particularly needed for oceanic microeukaryotes, which include a large diversity of poorly understood symbiotic rela- tionships that range from mutualistic to parasitic. The reason for the current state of affairs is that determining ecological interactions between microbes has proven to be highly challenging. How- ever, recent technological developments in genomics and transcriptomics (metaomics for short), coupled with microfluidics and high-performance computing are making it increasingly feasible to determine ecological interactions at the microscale. -
Insights Into the Dynamics Between Viruses and Their Hosts in a Hot Spring Microbial Mat
The ISME Journal (2020) 14:2527–2541 https://doi.org/10.1038/s41396-020-0705-4 ARTICLE Insights into the dynamics between viruses and their hosts in a hot spring microbial mat 1,2,11 1,2 1,2 1,2 1,2 Jessica K. Jarett ● Mária Džunková ● Frederik Schulz ● Simon Roux ● David Paez-Espino ● 1,2 1,2 1,2 3 4 Emiley Eloe-Fadrosh ● Sean P. Jungbluth ● Natalia Ivanova ● John R. Spear ● Stephanie A. Carr ● 5 6 7 8,9 1,2 Christopher B. Trivedi ● Frank A. Corsetti ● Hope A. Johnson ● Eric Becraft ● Nikos Kyrpides ● 9 1,2,10 Ramunas Stepanauskas ● Tanja Woyke Received: 13 January 2020 / Revised: 3 June 2020 / Accepted: 11 June 2020 / Published online: 13 July 2020 © The Author(s) 2020. This article is published with open access Abstract Our current knowledge of host–virus interactions in biofilms is limited to computational predictions based on laboratory experiments with a small number of cultured bacteria. However, natural biofilms are diverse and chiefly composed of uncultured bacteria and archaea with no viral infection patterns and lifestyle predictions described to date. Herein, we predict the first DNA sequence-based host–virus interactions in a natural biofilm. Using single-cell genomics and metagenomics – 1234567890();,: 1234567890();,: applied to a hot spring mat of the Cone Pool in Mono County, California, we provide insights into virus host range, lifestyle and distribution across different mat layers. Thirty-four out of 130 single cells contained at least one viral contig (26%), which, together with the metagenome-assembled genomes, resulted in detection of 59 viruses linked to 34 host species. -
Impact of Single-Cell Genomics and Metagenomics on the Emerging View of Extremophile ‘‘Microbial Dark Matter’’
Extremophiles DOI 10.1007/s00792-014-0664-7 SPECIAL ISSUE: REVIEW 10th International Congress on Extremophiles Impact of single-cell genomics and metagenomics on the emerging view of extremophile ‘‘microbial dark matter’’ Brian P. Hedlund • Jeremy A. Dodsworth • Senthil K. Murugapiran • Christian Rinke • Tanja Woyke Received: 2 April 2014 / Accepted: 5 June 2014 Ó Springer Japan 2014 Abstract Despite [130 years of microbial cultivation unique genomic features such as stop codon reassignments, studies, many microorganisms remain resistant to tradi- and predictions about cell ultrastructure. In addition, the tional cultivation approaches, including numerous candi- rapid expansion of genomic coverage enabled by these date phyla of bacteria and archaea. Unraveling the studies continues to yield insights into the early diversifi- mysteries of these candidate phyla is a grand challenge in cation of microbial lineages and the relationships within microbiology and is especially important in habitats where and between the phyla of Bacteria and Archaea. In the next they are abundant, including some extreme environments 5 years, the genomic foliage within the tree of life will and low-energy ecosystems. Over the past decade, parallel continue to grow and the study of yet-uncultivated candi- advances in DNA amplification, DNA sequencing and date phyla will firmly transition into the post-genomic era. computing have enabled rapid progress on this problem, particularly through metagenomics and single-cell Keywords Single-cell genomics Á Metagenomics Á genomics. Although each approach suffers limitations, Candidate phyla Á Genomic encyclopedia of bacteria and metagenomics and single-cell genomics are particularly archaea (GEBA) Á ‘‘Microbial dark matter’’ powerful when combined synergistically. -
Analysis of Codon Usage Patterns in Giardia Duodenalis Based on Transcriptome Data from Giardiadb
G C A T T A C G G C A T genes Article Analysis of Codon Usage Patterns in Giardia duodenalis Based on Transcriptome Data from GiardiaDB Xin Li, Xiaocen Wang, Pengtao Gong, Nan Zhang, Xichen Zhang and Jianhua Li * Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China; [email protected] (X.L.); [email protected] (X.W.); [email protected] (P.G.); [email protected] (N.Z.); [email protected] (X.Z.) * Correspondence: [email protected]; Tel.: +86-431-8783-6172; Fax: +86-431-8798-1351 Abstract: Giardia duodenalis, a flagellated parasitic protozoan, the most common cause of parasite- induced diarrheal diseases worldwide. Codon usage bias (CUB) is an important evolutionary character in most species. However, G. duodenalis CUB remains unclear. Thus, this study analyzes codon usage patterns to assess the restriction factors and obtain useful information in shaping G. duo- denalis CUB. The neutrality analysis result indicates that G. duodenalis has a wide GC3 distribution, which significantly correlates with GC12. ENC-plot result—suggesting that most genes were close to the expected curve with only a few strayed away points. This indicates that mutational pressure and natural selection played an important role in the development of CUB. The Parity Rule 2 plot (PR2) result demonstrates that the usage of GC and AT was out of proportion. Interestingly, we identified 26 optimal codons in the G. duodenalis genome, ending with G or C. In addition, GC content, gene expression, and protein size also influence G. -
Close Evolutionary Relationship Between Rice Black-Streaked Dwarf
Wang et al. Virology Journal (2019) 16:53 https://doi.org/10.1186/s12985-019-1163-3 RESEARCH Open Access Close evolutionary relationship between rice black-streaked dwarf virus and southern rice black-streaked dwarf virus based on analysis of their bicistronic RNAs Zenghui Wang1,2†, Chengming Yu2†, Yuanhao Peng1†, Chengshi Ding1, Qingliang Li1, Deya Wang1* and Xuefeng Yuan2* Abstract Background: Rice black-streaked dwarf virus (RBSDV) and Southern rice black-streaked dwarf virus (SRBSDV) seriously interfered in the production of rice and maize in China. These two viruses are members of the genus Fijivirus in the family Reoviridae and can cause similar dwarf symptoms in rice. Although some studies have reported the phylogenetic analysis on RBSDV or SRBSDV, the evolutionary relationship between these viruses is scarce. Methods: In this study, we analyzed the evolutionary relationships between RBSDV and SRBSDV based on the data from the analysis of codon usage, RNA recombination and phylogenetic relationship, selection pressure and genetic characteristics of the bicistronic RNAs (S5, S7 and S9). Results: RBSDV and SRBSDV showed similar patterns of codon preference: open reading frames (ORFs) in S7 and S5 had with higher and lower codon usage bias, respectively. Some isolates from RBSDV and SRBSDV formed a clade in the phylogenetic tree of S7 and S9. In addition, some recombination events in S9 occurred between RBSDV and SRBSDV. Conclusions: Our results suggest close evolutionary relationships between RBSDV and SRBSDV. Selection pressure, gene flow, and neutrality tests also supported the evolutionary relationships. Keywords: RBSDV, SRBSDV, Phylogenetic analysis, Recombination, Selection pressure Introduction was first reported in Guangdong Province, China in In major rice-growing regions, virus diseases occurred 2001 [4], and later was identified as a disease caused by frequently and caused severe damages to rice, among Southern rice black-streaked dwarf virus (SRBSDV), a which Rice black-streaked dwarf virus (RBSDV) and new member of the genus Fijivirus [5]. -
Challenging the Anthropocentric Emphasis on Phenotypic Testing in Prokaryotic Species Descriptions: Rip It up and Start Again
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Frontiers - Publisher Connector OPINION published: 17 June 2015 doi: 10.3389/fgene.2015.00218 Challenging the anthropocentric emphasis on phenotypic testing in prokaryotic species descriptions: rip it up and start again Iain C. Sutcliffe * Applied Sciences, Faculty of Health and Life Sciences, Northumbria University, Newcastle upon Tyne, UK Keywords: genomics, phylogeny, species, systematics, taxonomy “The benefits of specialization are tempered by the possibility that specialized groups become isolated, resist innovation, and engage in destructive competitiveness” (Specialized Science, Casadevall and Fang 2014). Prokaryotic systematics is a highly specialized field and yet has fundamental reach and significance given that it provides the framework and, importantly, the names which we use to describe most of the microbial world. Names are given to prokaryotic taxa under the jurisdiction of the International Code of Nomenclature of Prokaryotes (ICNP; Lapage et al., 1992) and the vast majority of papers in prokaryotic systematics are descriptions that name taxa, particularly novel species and genera. Edited by: Indeed, this century has seen a significant growth in the number of prokaryotic species named Radhey S. Gupta, (Tamames and Rosselló -Móra, 2012; Oren and Garrity, 2014), with ∼900 new names for Bacteria McMaster University, Canada and Archaea published (either validly or effectively) in 2014. Mostly this growth in taxonomic Reviewed by: activity is in Asia, with declines elsewhere (Tamames and Rosselló -Móra, 2012; Oren and Garrity, Ramon Rossello-Mora, 2014). Institut Mediterrani d’Estudis Despite this progress, prokaryotic systematics has become isolated from mainstream Avançats, Spain microbiology and resistant to innovation.