Glycoprotein and Proteoglycan Techniques

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Glycoprotein and Proteoglycan Techniques

View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector Volume 202, number 1 FEBS LETTERS June 1986 Glycoprotein and Proteoglycan Techniques Volume 16 in the Series ‘Laboratory Techniques in Biochemistry and Molecular Biology’ (General Editors: R.H. Burdon and P.H. van Knippenberg) by J.G. Beeley Elsevier; Amsterdam, New York, Oxford, 1985 x + 462 pages. $109.25, Dfl.295.00 (hardback); $31.50, Dfl. 85.00 (paperback) This book is the sixteenth in the series of on isolation and fractionation, specific examples laboratory technique handbooks. The editors have are given of the application of procedures to done well to select Dr Beeley for this arduous task specific problems such as the extraction of of producing such a comprehensive book on such glycophorin from red cells by two different a complex and rapidly growing area. methods, the extraction of band 3 glycoprotein, The book, apart from a brief introduction ex- the purification of Thy I antigen from thymocytes, plaining the general interest in glycoproteins, is of ovine submaxillary mucin, porcine gastric divided into seven main chapters: Glycoproteins mucus, of proteoglycan subunits and dermatan and proteoglycans; Isolation and fractionation; sulphate from cartilage and keratan sulphate from Physico-chemical characterisation; Analysis of cornea. I do not need to go into the details of each constituents; Structural analysis; Lectin tech- chapter, but I particularly appreciated sections on niques; Radioactive labelling techniques. the strategies for the structural analysis of The chapter on glycoproteins and proteoglycans oligosaccharides and the advice on the gives a very useful summary of the structures of chromatographic conditions for lectin affinity the glycosylated glycoproteins, the others dealing columns. with the methods by which these structures can be In summary this is an excellent comprehensive isolated and analysed. In each chapter the details handbook that should be in every laboratory where of the procedure are given such that they can be glycoproteins are isolated, investigated or understood and carried out, and complete analysed. references are given so that further details can be found if required. As an example, in the chapter Anthony K. Allen 161 .

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