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3 Wroclaw Scientific Meetings 3rd Wroclaw Scientific Meetings 3rd Wroclaw Scientific Meetings Editors: Julita Kulbacka Nina Rembiałkowska Joanna Weżgowiec Wroclaw 2019 3rd Wroclaw Scientific Meetings Wrocław, 1st-2nd March 2019 Editors: Julita Kulbacka Nina Rembiałkowska Joanna Weżgowiec Typesetting: Monika Maciąg Kamil Maciąg Cover design: Marcin Szklarczyk Nina Rembiałkowska © Copyright by Wydawnictwo Naukowe TYGIEL Sp. z o.o. ISBN 978-83-65932-64-8 Publisher: Wydawnictwo Naukowe Tygiel Sp. z o.o. ul. Głowackiego 35/341, 20-060 Lublin www.wydawnictwo-tygiel.pl Scientific Committe: prof. Janina Kwiatkowska-Korczak Department of Medical Biochemistry, Wroclaw Medical University, Poland prof. Halina Grajeta Department of Food Science and Dietetics, Wroclaw Medical University, Poland prof. dr hab. Izabela Fecka Department of Pharmacognosy and Herbal Medicines, Wroclaw Medical University, Poland prof. Halina Milnerowicz Department of Biomedical and Environmental Analyses, Wroclaw Medical University, Poland prof. Bożena Karolewicz Department of Drugs Form Technology, Wroclaw Medical University, Poland prof. Mounir Tarek Centre National de La Recherche Scientifique (CNRS) Université de Lorraine, Nancy, France prof. Saulius Šatkauskas Department of Biology, Vytautas Magnus University in Kaunas, Lithuania prof. Marcin Drąg Department of Bioorganic Chemistry, Faculty of Chemistry, Wroclaw University of Science and Technology Biochemistry, Wroclaw, Poland prof. dr hab. Agnieszka Piwowar Department of Toxicology, Wroclaw Medical University, Poland dr hab. Jolanta Saczko, prof. nadzw. Department of Molecular and Cellular Biology, Wroclaw Medical University, Poland dr hab. inż. Julita Kulbacka Department of Molecular and Cellular Biology, Wroclaw Medical University, Poland dr hab. Iwona Bil-Lula Department of Medical Laboratory Diagnostics, Wroclaw Medical University, Poland dr Agnieszka Chwiłkowska Department of Molecular and Cellular Biology, Wroclaw Medical University, Poland dr Helena Moreira Department of Basic Medical Sciences, Wroclaw Medical University, Poland dr inż. Nina Rembiałkowska Department of Molecular and Cellular Biology, Wroclaw Medical University, Poland dr inż. Dagmara Baczyńska Department of Molecular and Cellular Biology, Wroclaw Medical University, Poland dr inż. Anna Choromańska Department of Medical Biochemistry, Wroclaw Medical University, Poland dr inż. Joanna Rossowska Institute of Immunology and Experimental Therapy, Poland dr Vitalij Novickij Institute of High Magnetic Fields, Vilnius Gediminas Technical University, Lithuania dr Małgorzata Drąg-Zalesińska Department of Human Morphology and Embryology, Wroclaw Medical University, Poland Organizing Committe: dr hab. Jolanta Saczko, prof. nadzw. Department of Medical Biochemistry, Wroclaw Medical University, Poland prof. dr hab. Agnieszka Piwowar Department of Toxicology, Wroclaw Medical University, Poland prof. dr. hab. Adam Matkowski Department of Biology and Pharmaceutical Botany, Wroclaw Medical University, Poland dr hab. inż. Julita Kulbacka Department of Medical Biochemistry, Wroclaw Medical University, Poland dr hab. Ewa M. Kratz Department of Laboratory Diagnostics,Wroclaw Medical University, Poland dr inż. Nina Rembiałkowska Department of Medical Biochemistry, Wroclaw Medical University, Poland dr inż. Dagmara Baczyńska Department of Molecular and Cellular Biology, Wroclaw Medical University, Poland dr Agnieszka Chwiłkowska Department of Medical Biochemistry, Wroclaw Medical University, Poland dr inż. Anna Choromańska Department of Medical Biochemistry, Wroclaw Medical University, Poland dr Helena Moreira Department of Basic Medical Sciences, Wroclaw Medical University, Poland dr Ewa Sawicka Department of Toxicology, Wroclaw Medical University, Poland dr inż. Joanna Weżgowiec Department of Experimental Dentistry, Wroclaw Medical University, Poland dr inż. Joanna Rossowska Institute of Immunology and Experimental Therapy, Poland dr Anna Szewczyk Department of Molecular and Cellular Biology, Wroclaw Medical University, Poland dr Adriana Kubis-Kubiak Department of Toxicology, Wroclaw Medical University, Poland dr Beata Szymańska Department of Toxicology, Wroclaw Medical University, Poland mgr Olga Michel Department of Medical Biochemistry, Wroclaw Medical University, Poland mgr Katarzyna Bieżuńska-Kusiak Department of Medical Biochemistry, Wroclaw Medical University, Poland Karolina Tądel Wroclaw Medical University, Poland Marek Kulbacki DIVE IN AI, Polish Japanese Academy of Information Technology, Poland Orgnizers: Honorary Patronage: Sponsors: Content: CONFERENCE PROGRAMM ...................................................................... 15 LECTURES ................................................................................................... 19 ORAL PRESENTATIONS ............................................................................ 27 POSTER PRESENTATIONS ........................................................................ 53 INDEX OF AUTHORS ................................................................................ 187 Dear Sir or Madam, This is the third scientific meeting in Wroclaw. This year the Department of Molecular and Cellular Biology together with the Department of Toxicology has already organized the conference entitled "3rd Wroclaw Scientific Meetings", dedicated to young scientists. According to tradition, the aim of the conference is to exchange information about scientific research between young people, enthusiasts of science from many centers in Poland and abroad. The conference is multidisciplinary and covers broadly understood cell biology in the field of drug interactions in cancer and normal cells, cell-drug interaction, cellular response to oxidative stress, cellular engineering, including computer modeling. During the conference, young scientists will have the opportunity to present the results of their research and discuss them with experienced and valued people of science, who were invited to participate in this year's conference. I would like to emphasize that this year's plenary lectures will be presented by world-renowned scientists from Poland and abroad. During the conference, practical workshops on the flow cytometry will also be organized. I cordially invite you to take part in them. I wish all conference participants fruitful discussions and nice moments in Wrocław. We are very happy that our conference meets with growing interest of young science enthusiasts not only from Poland but also from foreign centers. Dr hab. Jolanta Saczko, prof. nadzw. CONFERENCE PROGRAMM 01.03.2019 Friday 08:00-10:00 Registration 10:15-10:30 Opening ceremony – Prof. Jolanta Saczko and Prof. Halina Grajeta 1st SESSION Moderators: Marcin Drąg and Jolanta Saczko 10:30-11:00 Lecture I of Prof. Marcin Drąg (Wroclaw University of Science and Technology, Wroclaw, Poland) “In Quest of Optimal Technology to Obtain Selective Activity-Based Probes for Investigation of Proteases in Cell Death” 11:00-11:12 Dominika Benkowska “Lyotropic liquid crystals in biological systems” 11:13-11:24 Klaudia Bielak “Otolin-1 – expression and purification of the structural protein of fish otoliths and vertebrate otoconia” 11:25-11:36 Ewa Kątnik “The role of BCL11A protein in non-small cell lung cancer” 11:37-11:48 Krzysztof Kotowski “PFKFB3 inhibition by use of 3PO induce apoptosis and cell cycle arrest in A375 human melanoma cell Line” 11:49-12:00 Marta Piksa “Photosensitizers as anti-inflammatory agents in the diabetic foot” 12:00-12:30 Coffee break and POSTER SESSION 15 2nd SESSION Moderators: Joanna Rossowska and Helena Moreira 12:30-13:00 Lecture II of Dr. Joanna Rossowska (Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland) “Flow cytometry applications in medical and biological sciences” 13:00-13:15 Dawid Lupa “Noble metal nanoparticles monolayers at colloidal carriers - novel systems for controlled release” 13:15-13:30 Matylda Niedziela “Influence of Pelargonium sidoides proanthocyanidins on metabolism and apoptosis of Dental Pulp Stem Cells” 13:30-13:45 Magdalena Surman “Comparison of surface glycosylation of melanoma cells and melanoma-derived ectosomes” 13:45-14:00 Dries Van Elst “Mechanisms and novel treatment approaches in allergen immunotherapy” 14:00-15:30 Lunch and POSTER SESSION 14:15-15:45 Flow Cytometry Workshops (Two groups 14:15-15:00, II – 15:00-15:45) 3rd SESSION Moderators: Mounir Tarek and Łukasz Radosiński 15:45-16:15 Lecture III of Prof. Mounir Tarek (CNRS- University de Lorraine, Nancy, France) “Drug delivery through cell membranes: What can we learn from Molecular Dynamics simulations” 16:15-16:27 Łukasz Lewandowski “Organs-on-a-chip: an innovative tool in toxicological studies” 16:28-16:39 Wojciech Szlasa “How to predict the secondary from the primary structure of the protein?” 16 16:40-16:51 Natalia Szulc “Calcium electroporation- a molecular dynamics simulation” 16:52-17:03 Sebastian Szymański “Hypericin and phenothiazines – the use of computational methods to determine the structure and properties of drugs” 17:04-17:15 Daniel Wiczew “Pro-oxidative properties of cynamonic acid towards normal and cancer human cells” 17:15-17:45 POSTER SESSION – diner & discussions 02.03.2019 Saturday 4th SESSION Moderators: Saulius Šatkauskas and Julita Kulbacka 9:00-9:30 Lecture IV of Prof. Saulius Šatkauskas (Vytautas Magnus University, Kaunas, Lithuania) “Cell sonoporation: from basics to targeted drug delivery” 9:30-9:42 Ludmila Bogacz-Radomska “Carotenoids as Source of Antioxidant Acitivity in Food Matrix”
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