EVPC STANDARD RESIDENCY TRAINING PROGRAM AT THE UNIT OF AND PARASITIC DISEASES, DEPARTMENT OF AND ANIMAL PRODUCTIONS, UNIVERSITY OF NAPLES FEDERICO II, ITALY

Frederick II and the hawk from “De arte venandi cum avibus”

University of Naples, Federico II Unit of Parasitology and Parasitic Diseases Department of Veterinary Medicine and Animal Productions Via Federico Delpino 1, Naples, Italy Tel: +390812536283 Tel: +390812536281

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DESCRIPTION OF THE TRAINING PROGRAM

A. GENERAL DESCRIPTION Programe Director: Univ. Prof. Dr. Med. Vet., DipEVPC Giuseppe Cringoli (Head of the Unit of Parasitology and Parasitic Diseases) Contact details: Unit of Parasitology and Parasitic Diseases Department of Veterinary Medicine and Animal Productions University of Naples Federico II Via Federico Delpino 1 80137 Naples, Italy Tel: +39 0812536283 http://www.parassitologia.unina.it Email: [email protected]

The supervisor will be assisted at this location by one Asssociate member of EVPC: Prof. Laura Rinaldi, PhD Contact details: Unit of Parasitology and Parasitic Diseases Department of Veterinary Medicine and Animal Productions University of Naples Federico II Via Federico Delpino 1 80137 Naples, Italy Tel: +39 0812536281 Email: [email protected]

Name and qualification of mentors: Prof. Edwin Claerebout DVM, PhD, DipEVPC Laboratory of Parasitology; Department of Virology, Parasitology and Immunology; Faculty of Veterinary Medicine; Ghent University, Belgium

Prof. Laura Helen Kramer DVM, PhD, DipEVPC Laboratory of Veterinary Parasitology, Department of Medical Veterinary Science, University of Parma, Italy

Dr. Luigi Venco, DVM, PhD, DipEVPC Veterinary Hospital “Città di Pavia” in the fields of Cardiology (both medicine and surgery) and Clinical Parasitology, Pavia, Italy

Prof. Peter Deplazes, DVM, PhD, DipEVPC Director of the Institute of Parasitology, Vetsuisse Faculty, University of Zurich, Switzerland

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Prof. Smaragda Sotiraki, DVM, PhD, DipEVPC Veterinary Research Institute, Hellenic Agricultural Organisation- Demeter (former NAGREF), Thessaloniki, Greece

1. Finance: There is no financial support for the residency position

Requirements for entry into the programme: The candidate must be licensed to practice veterinary medicine in the countries of the European Union or be relieved of this obligation by the EVPC Board. A completion of one-year internship is requires: this will be a flexible rotating clinical training in veterinary medicine beyond the professional degree. It provides practical experience in applying knowledge gained during formal professional education,and offers an opportunity for recent graduates to obtain additional training in the clinical and basic sciences. An internship is composed of a broad range of clinical assignments within one of the major divisions of veterinary medicine. This year of comprehensive broad postgraduate training and experience prepares a for high-quality service in practice or for a decision on an area of specialisation. A one-year internship programme is already ongoing at the Department of Veterinary Medicine and Animal Productions at the University of Naples Federico II and includes training in all the clinical and diagnostic units during 45 weeks. 2. Aims of Residency Training: To train the resident to a specialist level in Veterinary Parasitology over a three-year full time study period. The program is intended to allow the resident to apply for examination by the European Veterinary Parasitology College for Diplomate status. The resident will be expected to apply for the EVPC diploma examination. The expected outcomes of the SRTP are: - publication of at least three first-author refereed articles - at least 1 scientific presentation at a national or international Parasitology meeting - approval to sit the EVPC Diploma Examination

Pre-Program Training: If the resident candidate has participated in postgraduate training (e.g. doctoral or PhD program and post-doc) in parasitology or a related subject of veterinary sciences for at least 3 years, then the resident is not required to complete one-year internship before entering the Standard Residency Training Program.

3. Details of the Training Program • The program is supervised by an EVPC Diplomate, the Program Director (see A.) • The program will be structured so that the resident obtains training in all aspects of veterinary parasitology • The annual timetable for the program is: a) 2 weeks of training in another Unit of the Department of Veterinary Medicine and Animal Productions, University of Naples (parasitological cases in veterinary clinics and ). The training will include clinical examination of small animals, with emphasis on scopes of parasitological interest, e.g. dermatology and internal medicine. The resident will be advised in basic skills of imaging technologies. The pro-active participation of the resident is required to obtain a sufficient number of cases at the end of SRTP. SRTP Proposal Naples page 4 of 105

b) 26 weeks working in the diagnostic laboratory. On an annual basis about 5 clinical trials are conducted; they contribute 20 weeks to the diagnostic work. The program will include training in application, use and maintenance of the diagnostic equipment of the coprological, haematological, serological, entomological and molecular units of the diagnostic laboratory. This program will be accompanied by studies on parasite systematic, morphology, biology and epidemiology. It will also include clinical Parasitology, parasitic zoonoses and vector-borne diseases. The resident will also participate in the teaching of the undergraduate students in basic and clinical Parasitology and will be involved in the exams for Veterinary Parasitology, under the supervision of an EVPC Diplomate. c) 8 weeks of study. The resident has the possibility to attend several courses of further education in parasitology and related fields such as epidemiology, immunology and molecular biology. d) 12 weeks dedicated to research. The resident will develop a research project of his/her interest. The output of the research project will result in scientific manuscripts and national as well as international scientific meetings through oral or poster presentations. e) 4 weeks of externship to another institutions f) a minimum of 30 weekly seminars/journal club/slide sessions, etc has to be attended. The variety of species and wealth of knowledge available among the European Specialists based at the host and affiliated institutions will ensure that the resident should have challenging, stimulating and successful three-year program of study. g) The teaching activity of the Resident will include topics in clinical parasitology /laboratory diagnostic procedures. The teaching will be directed to: veterinary students, PhD students and veterinary practitioners. 1. Veterinary students This activity includes a lecture of 2 hours given once each semester. In addition students are taught in the laboratory during their clinical rotations on the university hospital (approximately 15 hours per semester). The teaching is focused on diagnosis of the most common parasitic infections in domestic animals, with emphasis on the adequate choice and the test characteristics of each diagnostic technique. Furthermore, this activity consists of practical microscopy sessions in which the students are assisted with performing the techniques. In addition the candidate is giving a lecture on PCR diagnostics of parasites. This lecture is of one hour duration and is given every semester. 2. Discussion of clinical cases with PhD students. The Resident will participate as mediator in the PhD journal club, approximately once a month for 3 hours, dealing with current research in veterinary parasitology. 3. Continuing education of . In addition to the activity within the University, in the third year of the programme, the Resident will participate as lecturer at CPD cpurses for practicing veterinarians. These talks, (approximately 5 lectures of a mean duration of three hours each) will typically focus on the choice and interpretation of diagnostic tools, regimens for antiparasitic treatment, species spectrum and mode of action of antiparasitic drugs. h) The amount of weeks for training must allow for time off in accordance with national legislation on work contracts. Italian national legislation foresees a total of five weeks vacation annually. Parental leave is approximately five months. According to the EBVS Policy and Procedures (2016) “In case of parental leave, illness or under exceptional circumstances a prolongation (of the Residency programme is possible. The duration of the prolongation is agreed with the College’s Credentials /Education committee.”

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B. SPECIFIC ACTIVITES

1. Techniques to be acquired: at the end of the three-year program the following techniques should be mastered: • Detection of ectoparasites: examination of skin scrapings (live/dead examination). • Detection of blood parasites: blood smears and stainings. • Coproscopical methods: - Qualitative faecal examination (flotation methods; sedimentation; combined sedimentation- flotation and Baermann technique; specific techniques e.g. autofluorescence, tapeworm egg examination in horses, soil sample preparation). - Quantitative faecal examination (FLOTAC technique, Mini-FLOTAC technique, McMaster technique, Fecpak technique and Wisconsin technique). • Culture of nematode larvae, larval differentiation. • Maintenance of ticks and endoparasites in vivo and in vitro (cell culture of , in vitro maintenance of nematode larvae, in vitro feeding of ticks, maintenance of model parasites in animal hosts for diagnosis, bioassays, basic research in immunology and cell biology). • Serological methods (ELISA-development and evaluation; IFAT, Western-Blot). • Methods of molecular biology (PCR and sequencing, Multiplex PCR, real-time PCR). • Geospatial tools (e.g. geographical information systems) for planning parasitological surveys, mapping and studying the epidemiology of parasites.

2. Rotational training: Each year the resident will attend a 2-week rotational training period at clinical Units of the Veterinary Medicine and Animal Productions in the following facilities: • Clinic for Ruminant • Clinic for Companion Animals • Laboratory of Pathology

3. Training programs: The resident will spend a training period each year under the supervision of EVPC Diplomates for further training in research on clinical parasitology of companion animals, large and small ruminants, serological, immunological and haematological diagnosis for parasites. During the whole training program a minimum of three months is designated for this specific training. Colleges focused for this training are:

• Laboratory of Parasitology; Department of Virology, Parasitology and Immunology; Faculty of Veterinary Medicine; Ghent University, Belgium Prof. Edwin Claerebout DVM, PhD, DipEVPC

• Laboratory of Parasitology, Department of Medical Veterinary Sciences, University of Parma, Italy Prof. Laura H. Kramer DVM, PhD, DipEVPC

• Veterinary Hospital “Città di Pavia” in the fields of Cardiology (both medicine and surgery) and Clinical Parasitology, Pavia, Italy Dr. Luigi Venco, DVM, PhD, DipEVPC

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• Institute of Parasitology, University of Zurich, Switzerland Prof. Peter Deplazes, DVM, PhD, DipEVPC

• Veterinary Research Institute, Hellenic Agricultural Organisation- Demeter (former NAGREF), Thessaloniki, Greece Prof. Smaragda Sotiraki, DVM, PhD, DipEVPC

Description of training externships:

1. University of Parma externship for EVPC SRTP at University of Naples The Resident will spend four weeks at the University of Parma Veterinary School and will attend the Veterinary Parasitology laboratory, headed by L. Kramer, as part of the EVPC Training activity. The Resident will obtain experience in the diagnosis, treatment and prevention of the Dirofilaria spp. in domestic animals. Topics will include: Dirofilaria immitis and D. repens (Knotts test, ELISA, cardiac Ultra Sound, clinical staging, alternative adulticide protocols, prevention); The Resident will also be asked to prepare a seminar for students on canine heartworm disease. 2. Ghent University, Belgium externship for EVPC SRTP at University of Naples

The Resident will spend four weeks at the Ghent University, Faculty of Veterinary Medicine, and will attend the Parasitology and Immunology laboratory, headed by Prof. Edwin Claerebout, as part of the EVPC. Training activity. The resident will obtain experience in the epidemiology, diagnosis and control of helminths, protozoa and ectoparasites of cattle. Topics will include: diagnosis and control of helminth infections in cattle; anthelmintic resistance in and Cooperia oncophora; epidemiology of and Cryptosporidium parvum; treatment and control of Psoroptes ovis in cattle; diagnosis and production effects of Ascaris suum in pigs; vaccine development against Ostertagia ostertagi and Cooperia oncophora and study of vaccine induced immune responses. Diagnostic activities: traditional parasitology (microscopy); serology (ELISA, IFAT, Western blot, immunolocalisation of proteins using immunofluorescence); molecular techniques (PCR, RT-PCR, cloning, sequencing). The Resident will also be asked to prepare a seminar for students on epidemiology and diagnosis of helminth and protozoa infections (Giardia duodenalis, Cryptosporidium parvum) in cattle. 3. University of Zurich, Switzerland externship for EVPC SRTP at the University of Naples

The resident will spend four weeks at the University of Zurich, Vetsuisse Faculty and will attend the Parasitology laboratory headed by Prof. Peter Deplazes. Training activity: the resident will obtain experience in transmitted zoonoses with focus on: transmission of in cats and the development of a vaccine; development of serological tests for Angiostrongylus vasorum; epidemiology, diagnosis and control of Echinococcus; vector .

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Topics will include: Toxoplasma gondii (morphological, immunological and molecular diagnostic techniques); in vitro and in vivo maintenance of parasite strains; development and validation of diagnostic tests; vector-entomology (morphological, immunological and molecular diagnostic techniques). The resident will also be asked to prepare two seminars for students on: 1) diagnosis and transmission of Toxoplasma gondii; 2) epidemiology, transmission and control of Echinococcus.

4. Veterinary Hospital “Città di Pavia”, Pavia, Italia externship for EVPC SRTP at University of Naples

The Resident will spend four weeks at the Veterinary Hospital “Città di Pavia” and will perform a clinical staging on small animals in a veterinary hospital, headed by Dr. Luigi Venco, as part of the EVPC. Training activity: the Resident will obtain experience in internal medicine on small animals (clinical examination and clinical diagnosis by laboratory methods); clinical parasitology and cardiology for diagnosis of heartworm infections. Topics will include: abdominal and cardiac sonography, radiology, fluoroscopy, CT scan; haematology, biochemistry, cytology; coprological standard examinations, skin scrapings, identification of ectoparasites; rapid ELISA tests, modified Knott’s test, hematological and cytological exams of parasitic interest; surgical heartworm removal via jugular vein. The Resident will also be asked to prepare a seminar for students describing clinical cases with focus on clinical parasitology performed during the externship.

5. Veterinary Research Institute, Hellenic Agricultural Organisation/DEMETER, Thessaloniki (GR) externship for EVPC SRTP at University of Naples

The Resident will spend four weeks at the Veterinary Research Institute and will attend the Veterinary Parasitology laboratory, headed by Prof. Smaragda Sotiraki, as part of the EVPC. Training activity. The Resident will obtain experience in the diagnosis, treatment and prevention of coccidian infection (Eimeria, Isospora) in sheep, goats and swine and in the diagnosis and treatment of gastrointestinal nematodes (e.g. Haemonchus contortus and colubriformis) in small ruminants. Topics will include: field activities on farms; diagnosis and control of coccidian and gastrointestinal nematode infections by flotation with different flotation solutions (sodium chloride, zinc sulphate, sucrose), sedimentation, McMaster, Baermann technique, immunofluoresence for Giardia and Cryptosporidium; ELISA for serology, PCR methods. The Resident will also be asked to prepare a seminar for students on diagnosis and treatment of coccidian and gastrointestinal nematode infections in small ruminants and swine.

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1st year of the SRTP • The resident will strengthen his/her knowledge of parasite systematics, morphology, biology and recognition of main parasites through personal study. • The resident will be trained in the application, use and maintenance of all the diagnostic equipment at the Diagnostic Laboratory of Parasitology and Parasitic Diseases. • The Resident will be required to attend clinical rotations and to begin keeping a Case Log of animals visited, diagnosed with and treated for parasitic diseases. • The resident will also collaborate in the undergraduate student teaching and the diagnostic services provided by the parasitology diagnostic laboratory, at first under strict supervision of a Diplomate, and more independently throughout the year. • The resident will attend courses in Epidemiology, Immunology and Pathology as applied to Veterinary Parasitology that will be arranged as part of the first year training support program. • The resident will choose a research topic of his/her interest and develop a research project: evaluation of the Resident’s critical analysis and verbal/written description of scientific knowledge will be a priority. • The resident will start a Case Log of animals visited, diagnosed with and treated for parasitic diseases, and the rotational training period • The resident, in collaboration with the supervising Diplomate, will select a research topic and develop a research project • The clinical or research activities should result in the preparation of one scientific paper by the end of the first year

2nd year of the SRTP • The resident will be further in clinical and diagnostic parasitology in the different parasitology diagnostic laboratory units. The resident will also participate to a larger extent in the undergraduate student teaching and the clinical services provided by the parasitology diagnostic laboratory, under the supervision of an EVPC Diplomate. • The resident will further strengthen his/her knowledge of clinical parasitology, parasitic zoonoses and vector-borne parasitic diseases in 8 weeks through personal study. • The resident will further strengthen his/her knowledge in molecular biology, immunology and cell biology. • An increase in responsibility for diagnostic parasitology will be required. • Participation in scientific meetings of national/international societies for parasitology and other continuing education meetings will be expected. • The resident will provide tutorials to undergraduate and post-graduate students, under the supervision of an EVPC Diplomate • The resident, in collaboration with the supervising Diplomate, will further develop the research project.

3rd year of the SRTP • As in the third year the resident will be further trained in the diagnostic parasitology and clinical parasitology. He/she will be also expected to participate in ungraduated and graduate teaching and the diagnostic services provided by the parasitology diagnostic laboratory, independently. SRTP Proposal Naples page 9 of 105

• At the end of the third year, all parasitological diagnostic techniques should be mastered for a wide range of parasites and the resident will expand his/her knowledge on parasite immunology and molecular biology of parasites. • The resident will further strengthen his/her knowledge of parasite systematics, morphology and biology and of clinical parasitology, parasitic zoonoses and vector-borne parasitic diseases in the 8 weeks of personal study. • The resident is expected to complete his Case Log, based on cases encountered both in the parasitology diagnostic laboratory and the rotational training periods. • The resident, in collaboration with the supervising Diplomate, will finalize the research project. • By the end of the third year of the SRTP, the activities in the diagnostic parasitology laboratory and the research activity should result in the preparation of one or two case reports and at least two research papers as first author, to a final number of at least three accepted manuscripts. • The resident is expected to prepare his/her application for approval to sit the EVPC Diploma examination.

C. LOCATION AND STAFF INVOLVED IN THE SRTP • Location: Department of Veterinary Medicine and Animal Productions, University of Naples, Federico II, Napoli, Italy • Duration 3 years (156 weeks) • Name and qualification of the Supervisors: • Prof. Dr. Giuseppe Cringoli, EVPC Diplomate • Prof. Dr. Laura Rinaldi, Associate Member of EVPC Staff: • 3 veterinarians (1 Post-Doc, 1 PhD student and 1 research fellow) • 1 biologists (1 Post-Doc) • 3 biotechnologists (2 Post-Doc and 1 PhD student) • 2 graduated in animal production sciences all of which are familiar with laboratory managment and safety policies through information sessions and periodical evaluation.

D. Description of the institution and learning environments

1. Description of physical facilities

1.1. Veterinary library and archives Library of the Department of Veterinary Medicine and Animal Productions containing textbooks and journals relating to Veterinary Parasitology and its supporting disciplines are readily accessible directly or electronically to the program participant. Additionally, full electronic access to the website concerning Veterinary Parasitology must be readily available-list which journals are accessible. The paper books and the multimedia materials are located in the borrowing point service in the building in via Delpino, 1. The books chosen for the classes are available at the head office and there are some 2500. The library sections hold books, some rare and precious editions. SRTP Proposal Naples page 10 of 105

According to a rough estimate, the total numbers of books is about 10.000, all searchable in the University OPAC (www.opac.unina.it). E-books concerning Veterinary Medicine are available in full-text in the CAB and CRC Press collections on CAB website, linked from the Library webpages too.

1.2 Clinical and necropsy facilities at the Department of Veterinary Medicine and Animal Productions of Naples The Department of Veterinary Medicine and Animal Productions is located in the city of Naples (Campania Region, southern Italy), close to the motorway and to the international airport of Capodichino (Naples). It is divided into an historical site located in via F. Delpino easily accessible by bus, car and subway, a Veterinary teaching hospital (VTH), a regional research center for Monitoring of Parasitic Diseases (CREMOPAR) located in Eboli (Salerno province), and an experimental centre for avian and rabbit diseases (CSA) located in Varcaturo (Naples province). The Department of Veterinary Medicine and Animal Productions offers services which are based on scientific research in the field of veterinary medicine. The Department of Veterinary Medicine and Animal Productions has been approved by the European Association of Establishments for (EAEVE) in 2013. On campus, a teaching hospital is available for small and large animals, as well as an ambulatoryservice and facilities for necroscopy and histology.

1.3 Laboratory facilities at the Unit of Parasitology • The laboratory consists of one EVPC Diplomate (DVM), one Associate Member of EVPC (Biologist, PhD in Veterinary Sciences), three non Diplomate veterinarians, one biologist, three biotechnologists, two graduated in animal sciences, all of which are familiar with laboratory management and safety policies through information sessions and periodical evaluation. At the Laboratory of Parasitology, we provide diagnostic services for a vast variety of parasites, including highly specialised diagnostics. The laboratory is currently expanding on its research in parasites epidemiology (including emerging infections), genetic diversity, and the role of different parasites in health and disease. The use of FLOTAC, Mini-FLOTAC and DNA-based technologies for diagnosis and geospatial tools for epidemiology plays a central role in our research as well as in our service work. The laboratory collaborates with a variety of national and international partners and the primary focus is the continuous development and optimisation of diagnostic methods. • There are three main points of diagnostic activity in parasitology: 1) Laboratories of Parasitology and Parasitic Diseases located in the Department 2) Laboratory of Parasitology and Parasitic Diseases located in the VTH 3) Laboratories of Parasitology and Parasitic Diseases located at CREMOPAR. The laboratories from the Department and VTH provide diagnostic services for companion animals (dogs, cats, exotic );qualitative and quantitative copromicroscopy using the innovative and multivalent FLOTAC techniques (Cringoli et al., Nature Protocols, 2010) are routinely performed. In addition, urological and haematological diagnosis (smears and modified Knott’s test), diagnostic tests based on immunofluorescence, immunohistochemistry and immunocromatography, dermatological diagnosis (skin scrapes, hair samples), identification of endo/ ectoparasites by microscopy and PCR-based tools are provided. Diagnostic activities in parasitology and parasitic diseases of equines, swine, poultry, rabbits and ruminants (sheep, goats, cattle and buffaloes) are mainly performed at CREMOPAR where there SRTP Proposal Naples page 11 of 105

are laboratories of protozoology, helmintology and entomology provided with last generation equipment. Written operation procedures for each procedure commonly used in the laboratory and biosecurity guidelines are provided in each laboratory. • A collaboration concerning both teaching and research exists with The Experimental Zooprophylactic Institute of Southern Italy (IZSM). The IZSM is one out of ten Zooprophylactic institutes present in Italy. The IZSM is a public health entity with an independent technical and administrative management, operating within the National Health Service for hygiene and , technical and scientifical arm of the State and the Regions of Campania and Calabria, with outlying sections in almost all provinces of the two regions. It is an important diagnostic and research institution providing veterinarians and animal breeders with consultations and laboratory analysis. • Laboratories of foreign countries with a close collaboration or in which researchers have been working in recent years:

a. Department of Farm Animal Health and Public Health- Nantes-Atlantic National College of Veterinary Medicine, Food Science and Engineering – ONIRIS, Nantes, France (Prof. Cristophe Chartier) b. School of Clinical Veterinary Science, University of Bristol, England (Prof. Gerald Coles and Prof. Eric Morgan) c. Laboratory of Parasitology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium (Prof. Jozef Vercruysse and Prof. Edwin Clarebout) d. Institute of Parasitology, University of Zurich, Switzerland (Prof. Peter Deplazes) e. Animal Health DepartmenVeterinary Teaching Hospital, Veterinary Faculty, Universidad Complutense of Madrid, Spain (Prof. Guadalupe Mirò). f. Victor Caeiro Laboratory of Parasitology, ICAAM – Instituto de Ciências Agrárias e Ambientais Mediterrânicas – Universidade de Évora – Núcleo da Mitra, Évora, Portugal (Prof. Helder Cortes) g. Department of Public Health and Epidemiology, Swiss Tropical and Public Health Institute, Basel, Switzerland (Prof. Jurg Utzinger) h. Institute of Tropical Medicine, University of Tubingen, Germany (Dr. Carsten Kohler)

2. Further description of laboratory units: • The Veterinary Teaching Hospital (VTH) is located in Cupa del Principe street, Naples, very close to the metro station. VTH provide training on both pet and wild animals. The VTH is a recently constructed hospital of 1,100 square meters which employs a team of 40 veterinarians of the Local Health Service (ASL), open 24 hours a day, to treat dogs, stray cats and wild animals (mainly birds); the affected animals are monitored by the diseases with particular regard to zoonosis. • The CREMOPAR was established by a resolution of the Campania Region (GR No. 5147, October 20th, 2000) and is actually governed by an agreement between the Department of Veterinary Medicine and Animal Productions, University of Naples Federico II and both the Agricultural and Veterinary Sectors of the Campania Region. The direction of the Centre is SRTP Proposal Naples page 12 of 105

entrusted to Prof. Giuseppe Cringoli. CREMOPAR includes FLOTAC laboratories for coprology and laboratories of Helminthology, Protozoology and Entomology, equipped with modern instruments. Furthermore, CREMOPAR is provided of a necropsy room for post- mortem diagnosis of parasites and a conference center (100 seats) for didactic activities. It plays an important driving force for the development of knowledge in the field of parasitology and in the field of animal production and veterinary public health. Diagnosis of parasitic infections in sheep, goats, cattle, buffaloes, horses, pigs, poultry and rabbits from the Campania Region and other areas of central and southern Italy are daily performed at CREMOPAR. Furthermore, research activities are carried out at CREMOPAR, especially regarding spatial epidemiology for a better knowledge of the spatial patterns of parasites through the use of modern and powerful resources provided by Geographic Information Systems (GIS) and other geospatial tools. For all the diagnostic activities ongoing at the three locations described above, the diagnostic activities in parasitology represent a needful teaching environment for the resident training on parasitic diseases of companion animals, and wildlife.

The resident will have further access to the core facility at the University of Naples where modern equipment for the analysis of biological material (DNA, RNA) is provided. Cooperation with the research groups at the affiliated institutions will complement the training program.

3. Accreditation The laboratory is ISO-certified (ISO 9001-2015) for quality management in Veterinary Parasitology since 2011.

4. Case load per year: Samples are submitted by the faculty teaching hospitals, by animal owners and by veterinary practitioners. Coproscopical examination: an average of 39696 diagnostic samples are examined:8520 cattle, 3060 buffaloes, 22580 sheep, 4820 are goats, 83 are equids, 17 are pigs, 480 from dogs or cats, 20 from lagomorphs, 86 from wild animals, 30 exotic animals. Ectoparasite diagnosis: an average of 1500 skin scrapings for the diagnosis of mange are examined. A total of about 500 ticks, 100 fleas and 100 lice are identified. Haematology: an average of 400 samples are examined for diagnosis of microfilariae in dogs and of blood protozoa in farm and companion animals. Immunology (coproimmunology and serology): an average of 800 samples are examined for diagnosis of Giardia/Cryptosporidium, Leishmania, Angiostrongylus vasorum and Dirofilaria immitis. Molecular diagnosis : an average of 600 samples are examined for diagnosis of different parasites.

5. Research profile of Unit of Parasitology and Parasitic Diseases: The Unit of Parasitology and Parasitic Diseases (http://www.parassitologia.unina.it) has been active in veterinary parasitology for over 20 years. The laboratory is an active member of the Livestock Helminth Research Alliance (LiHRA) (www.lihra.eu); Prof. Laura Rinaldi is the vice-president of the LiHRA. Moreover the Unit of Parasitology and Parasitic Diseases is partner of the STRARWORMS project (https://www.starworms.org/partners) and of the COST Action TD1303 EurNegVec (http://www.eurnegvec.org/). SRTP Proposal Naples page 13 of 105

The main research activities of Unit of Parasitology and Parasitic Diseases include: - Providing skills on etiology, epidemiology, pathogenesis, symptoms, diagnosis and control of companion animals and livestock. - Development and standardization of innovative diagnostic techniques (FLOTAC, Mini-FLOTAC, Fill-FLOTAC) for human and animal endoparasites. - Study on faecal egg count and comparison between traditional and innovative techniques for the diagnosis of endoparasites in humans and animals. - Epidemiology (including the use of Geographical Information Systems, Remote Sensing and Spatial Analysis) of parasites of large and companion animals. - Development of prophylaxis, treatment and control of endo and ectoparasites of veterinary interest, with particular reference to those agents of zoonotic diseases.

• Procedures : - Flotation techniques (simple flotation, Wisconsin, McMaster, FLOTAC, Mini-FLOTAC) using different flotation solutions (NaCl, Zinc Sulphate, etc.) for uro-copromicroscopic diagnosis. - Sedimentation technique for copromicroscopic diagnosis. - Modified Knott test for microfilariae detection. - Baermann technique for lungworms larvae detection. - Immunological kits (ELISA, IFAT, immunocromatografic tests) for detection of parasites antigens/antibodies in faeces and sera. - Staining of fecal smears with modified Ziehl-Neelsen for Giardia and Cryptosporidium detection. - Staining of blood smears with Diff –Quick Kit for hemoparasites. - Direct examination of skin/ear scrapings. - Identification of adult endoparasites. - Identification of ectoparasites. - Conventional, nested, semi-nested, multiplex PCR and sequencing.

• Instruments: Standard laboratory equipment at laboratories of Unit of Parasitology and Parasitic Diseases at Department of Veterinary Medicine and Animal Productions (University of Naples Federico II), at CREMOPAR and at VTH , includes: - 2 Laminar flow cabinets - 2 Chemical cabinets - 14 Optical microscopes - 3 Optical microscopes with camera to capture images - 2 Stereo microscopes - 2 Immunofluorescence microscopes - 1 Incubator - 1 Spectrophotometer for ELISA - 7 Centrifuges - 1 Vacuum machine - 1 Thermocycler

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List of relevant peer –reviewed publication (2012-2016):

2016 1. Ciucă L, Genchi M, Kramer L, Mangia C, Miron LD, Prete LD, Maurelli MP, Cringoli G, Rinaldi L., 2016, Heat treatment of serum samples from stray dogs naturally exposed to Dirofilaria immitis and Dirofilaria repens in Romania, Vet Parasitol. 30;225:81-5. 2. Gualdieri L, Piemonte M, Alfano S, Maffei R, Della Pepa ME, Rinaldi L, Galdiero M, Galdiero M, Cringoli G, 2016, Imigrants living in an urban milieu with sanitation in Southern Itlay: persistence and transmission of intestinal parasites. Parasitol Res. 115(3):1315-23. 3. Condoleo R, Musella V, Maurelli MP, Bosco A, Cringoli G, Rinaldi L, 2016, Mapping, cluster detection and evaluation of risk factors of ovine toxoplasmosis in Southern Italy, Geospat Health. 31;11(2):432. 4. Coulibaly JT, Ouattara M, Becker SL, Lo NC, Keiser J, N'Goran EK, Ianniello D, Rinaldi L, Cringoli G, Utzinger J. Comparison of sensitivity and faecal egg counts of Mini-FLOTAC using fixed stool samples and Kato-Katz technique for the diagnosis of Schistosoma mansoni and soil- transmitted helminths. Acta Trop. 2016 Aug 30. pii: S0001-706X(16)30661-1. 5. Lima VF, Ramos RA, Lepold R, Cringoli G, Rinaldi L, Faustino MA, Alves LC. A comparison of mini-FLOTAC and FLOTAC with classic methods to diagnosing intestinal parasites of dogs from Brazil Parasitol Res. 2016 Apr;115(4):1737-9. 6. Ramos RA, Lima VF, Monteiro MF, Santana Mde A, Lepold R, Faustino MA, Rinaldi L, Cringoli G, Alves LC. New insights into diagnosis of Platynosomum fastosum (Trematoda: Dicrocoeliidae) in cats. Parasitol Res. 2016 Feb;115(2):479-82.

2015 1. Cuervo PF, Rinaldi L, Cringoli G., 2015, Modeling the extrinsec incubation of Dirofilaria immitis in South America based on monthly and continuous climatic data., Vet Parasitol. 15;209(1-2):70-5. 2. Rinaldi L, Biggeri A, Musella V, De Waal T, Hertzberg H, Mavrot F, Torgerson PR, Selemetas N, Coll T, Bosco A, Grisotto L, Cringoli G, Catelan D., 2015, Sheep and in Europe: the Gloworm experience., Geospat Health. 19;9(2):309-17. 3. Rinaldi L, Hendrickx G, Cringoli G, Biggeri A, Ducheyne E, Catelan D, Morgan E, Williams D, Charlier J, Von Samson-Himmelstjerna G, Vercruysse J., 2015., Mapping and modelling helminth infections in ruminants in Europe: experience from Gloworm., Geospat Health. 19;9(2):257-9. 4. Barda B, Albonico M, Ianniello D, Ame SM, Keiser J, Speich B, Rinaldi L, Cringoli G, Burioni R, Montresor A, Utzinger J., 2015., How long can stool samples be fixed for an accurate diagnosis of soil-transmitted helminth infection using Mini-FLOTAC?, PLoS Negl Trop Dis. 7;9(4):e0003698. 5. Ramos RA, Lima VF, Monteiro MF, Santana Mde A, Lepold R, Faustino MA, Rinaldi L, Cringoli G, Alves LC., 2015., New insights into diagnosis of Platynosomum fastosum (Trematoda: Dicrocoeliidae) in cats. Parasitol Res. ;115(2):479-82. 6. Del Prete L, Maurelli MP, Pennacchio S, Bosco A, Musella V, Ciuca L, Cringoli G, Rinaldi L, 2015., Dirofilaria immitis and Angyostrongylus vasorum: the contemporaneous detection in kennels., BMC Vet Res. 21;11:305. SRTP Proposal Naples page 15 of 105

7. Pepe P, Castellano M, Alfano S, Della Pepa ME, Tirino V, Piemonte M, Desiderio V, Zappavigna S, Galdiero M, Caraglia M, Cringoli G, Rinaldi L, Galdiero M. induces autophagic vacuoles accumulation in human hepatocarcinoma cells. Vet Parasitol;212(3-4):175-80

2014 1. Rinaldi L, Cringoli G. Exploring the interface between diagnostics and maps of neglected parasitic diseases. Parasitology, 28:1-8. 2. d'Ovidio D, Rinaldi L, Ianniello D, Donnelly TM, Pepe P, Capasso M, Cringoli G., FLOTAC for diagnosis of endo-parasites in pet squirrels in southern Italy., Vet Parasitol.;200(1-2):221-4. 3. Maurelli MP, Rinaldi L, Alfano S, Pepe P, Coles GC, Cringoli G. Mini-FLOTAC, a new tool for copromicroscopic diagnosis of common intestinal nematodes in dogs. Parasit Vectors.;7:356. 4. Maesano G, Capasso M, Ianniello D, Cringoli G, Rinaldi L.Parasitic infections detected by FLOTAC in zoo mammals from Warsaw, Poland., Acta Parasitol.;59(2):343-53.

2013 1. Dipineto L, Rinaldi L, Bosco A, Russo TP, Fioretti A, Cringoli G. Co-infection by Escherichia coli O157 and gastrointestinal strongyles in sheep. Vet J.;197(3):884-5. 2. Barda BD, Rinaldi L, Ianniello D, Zepherine H, Salvo F, Sadutshang T, Cringoli G, Clementi M, Albonico M. Mini-FLOTAC, an innovative direct diagnostic technique for intestinal parasitic infections: experience from the field., PLoS Negl Trop Dis.;7(8):e2344. 3. Dipineto L, Borrelli L, Pepe P, Fioretti A, Caputo V, Cringoli G, Rinaldi L. Synanthropic birds and parasites. Avian Dis. ;57(4):756-8. 4. Albonico M, Rinaldi L, Sciascia S, Morgoglione ME, Piemonte M, Maurelli MP, Musella V, Utzinger J, Ali SM, Ame SM, Cringoli G. Comparision of three copromicroscopic methods to assess albendazole efficacy against soil-transmitted helminth infections in school-aged children on Pemba Island., Trans R Soc Trop Med Hyg. ;107(8):493-501.

2012 1. Rinaldi L, Gonzalez S, Guerrero J, Aguilera LC, Musella V, Genchi C, Cringoli G. A One- Health integrated approach fascioliasis in the Cajamarca valley of Peru., Geospat Health. ;6(3):S67-73. 2. Rinaldi L, Mihalca AD, Cirillo R, Maurelli MP, Montesano M, Capasso M, Cringoli G. FLOTAC can detect parasitic and pseudoparasitic elements in reptiles., Exp Parasitol. 130(3):282-4. 3. Steinmann P, Cringoli G, Bruschi F, Matthys B, Lohourignon LK, Castagna B, Maurelli MP, Morgoglione ME, Utzinger J, Rinaldi L. FLOTAC for the diagnosis of Hymenolepis spp. infection: proof-of-concept and comparing diagnostic accuracy with other methods., Parasitol Res. 111(2):749-54.

• List or (website) with relevant funded research projects The Unit of Parasitology and Parasitic Diseases participated at different international projects: - EU FP Projects PARASOL (FP6 - Project FOOD-CT-2005-02285) - GLOWORM (FP7-KBBE-2011-5) SRTP Proposal Naples page 16 of 105

- COST Actions CAPARA (FA COST Action FA0805) - EurNegVec (BMBS COST Action TD1303)

E. APPENDICES: Appendix 1: Description of Facilities, services and equipment of the University of Ghent (Belgium), Faculty of Veterinary Medicine; CV and publication list of Prof. Edwin Claerebout Appendix 2: Description of Facilities, services and equipment of the University of Parma (Italy), Vet School; CV and publication list of Prof. Laura Helen Kramer Appendix 3: Description of Facilities, services of the Clinic for companion animals “Città di Pavia”, Pavia (Italy); CV and publication list of Dr. Luigi Venco Appendix 4: Description of Facilities, services and equipment of the Institute of Parasitology, Vetsuisse Faculty, University of Zurich (Switzerland); CV and publication list of Prof. Peter Deplazes Appendix 5: Description of Facilities, services and equipment of the Laboratory of Parasitology, Veterinary Research Institute, Hellenic Agricultural Organisation- Demeter (former NAGREF), Thessaloniki (Greece); CV and publication list of Prof. Smaragda Sotiraki Appendix 6: CV and Publication List of the Program Director, Prof. Giuseppe Cringoli Appendix 7:CV and Publication list of Prof. Laura Rinaldi (Associate member EVPC)- Supervisor of the SRTP

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Appendix 1: Description of Facilities, services and equipment for the University of Ghent (Belgium), Faculty of Veterinary Medicine; CV and publication list of Prof. Edwin Claerebout

Contact: Prof. Edwin Claerebout DVM, PhD, DipEVPC Laboratory of Parasitology; Department of Virology, Parasitology and Immunology; Faculty of Veterinary Medicine; Ghent University, Belgium

The laboratory of Parasitology The Laboratory of Parasitology (www.vetparasitology.ugent.be) has been active in veterinary parasitology for over 30 years. During that time extensive expertise has been acquired on miscellaneous topics (see further). The laboratory staff consists of 3 full-time tenured academic staff (E. Claerebout; P. Geldhof, B. Levecke) and one visiting professor (P. Dorny), of which 2 are EVPC diplomates (E. Claerebout, P. Dorny), 4 post-doctoral researchers, 12 PhD students and 3 lab technicians, all of which are familiar with laboratory management and safety policies through information sessions and periodical evaluation. The laboratory is an active member of Provaxs, the Centre for Strategic Prophylaxis and Vaccine Development of Ghent University (www.provaxs.be) and the Livestock Helminth Research Alliance (www.lihra.eu) and is recognised as a WHO Collaboration Centre for the monitoring of anthelmintic drug efficacy for soil-transmitted helminthiasis (J. Vercruysse, B. Levecke). The main research activities of the Laboratory of Parasitology include - Diagnosis and control of helminth infections in cattle - Development of vaccines against Ostertagia ostertagi and Cooperia oncophora in cattle - Host-parasite interactions in Ostertagia ostertagi, Cooperia oncophora and Giardia duodenalis infections in cattle and Ascaris suum in pigs - Anthelmintic resistance in Ostertagia ostertagi and Cooperia oncophora in cattle - Molecular epidemiology of Giardia duodenalis and Cryptosporidium parvum - Treatment and control of Psoroptes ovis in cattle. - Communication strategies to improve farmers’ adoption potential for sustainable parasite control measures. - Soil transmitted helminths in humans This results in a scientific output of more than 20 scientific papers per year.

The current facilities comprise 2 principal laboratory units, which have been occupied in 1994 and 2015 and have modern research facilities.

1. The laboratory unit for applied parasitology (1994) is used as a diagnostic laboratory, for training of veterinary students and for clinical trials. This laboratory unit contains: • Standard laboratory equipment, including: - 6 stereo-microscopes - 12 microscopes of which 3 have immersion lenses and 1 is graduated - an immunofluorescence microscope with Leica live imaging system • Written standard operation procedures (SOP) for each procedure commonly used in the laboratory SRTP Proposal Naples page 18 of 105

• Background information for diagnosis on a wide range of parasites, accessible for all staff in the laboratory. These include books, reference manuals, retrievable specimen archives, an electronic database (photos), and access to internet.

2. The molecular parasitology and proteomic research unit is situated in the new Veterinary Research Building (2015) and is used for several research projects (see also www.vetparasitology.ugent.be) and for diagnostic purposes (serology). It is well equipped both for molecular and proteomic research. Frequently used proteomic techniques are chromatographic techniques (size-exclusion, ion exchange and affinity chromatography), 1D and 2D gel electrophoresis, Western blotting, immunolocalisation of proteins using immunofluorescence and confocal microscopy. Frequently used molecular techniques are PCR, (real-time) RT-PCR, cloning, recombinant expression and sequencing. Standard laboratory equipment also includes an Elisa- washer and Elisa-reader for detection of parasite-specific antibodies in serum, milk and meat juice samples. Written SOPs for each procedure that is commonly used and biosecurity guidelines are present in the laboratory.

The library The library of the Laboratory of Parasitology has more than 270 textbooks on Veterinary Parasitology and supporting disciplines, and has a subscription for 16 Parasitology journals. Audiovisual information is also accessible in the library. On the campus site there is also a faculty library, with access to more than 1800 books and more than 300 journals on miscellaneous topics in veterinary medicine. All staff has also full electronic access to several search engines and publishing offices, and to websites concerning veterinary parasitology.

Case load In the diagnostic laboratory an average of 1,500 diagnostic samples are examined each year, of which approximately 700 are bovine, 250 are equine, 250 samples from companion animals, and another 250 from miscelleneous hosts. Other animal species include small ruminants, reptiles, camelids, poultry and birds. These samples are submitted by the faculty teaching hospitals and by vets in practice.

CV and publication list Prof. Edwin Claerebout Date/Place of Birth: May 13nd, 1965/Ghent (Belgium) Nationality: Belgian

Education: Doctor in Veterinary Medicine (DVM), 1990, Ghent University, Belgium. PhD in Veterinary Sciences (Parasitology), 1998, Ghent University, Belgium. Diplomate of the European Veterinary Parasitology College (EVPC), 2003

Employment: 1990-1991: Veterinary Surgeon at Ramsey and Harding , Coleraine, Northern Ireland, UK 1991-1992: Veterinary Officer, Military Service SRTP Proposal Naples page 19 of 105

1992-1992: Junior assistant, Laboratory for Parasitology, Faculty of Veterinary Medicine, Ghent University 1992-1998: Assistant, Laboratory for Parasitology, Faculty of Veterinary Medicine, Ghent University 1998-2001: Post-doctoral researcher, Laboratory for Parasitology, Faculty of Veterinary Medicine, Ghent University 2001-2005: Lecturer, Laboratory for Parasitology, Faculty of Veterinary Medicine, Ghent University 2005-2010: Senior lecturer, Laboratory for Parasitology, Faculty of Veterinary Medicine, Ghent University 2010-present: Full professor, Laboratory for Parasitology, Faculty of Veterinary Medicine, Ghent University

Research experience in Veterinary Parasitology • Expertise in epidemiology, diagnosis, treatment and control of parasitic diseases in domestic animals. • Research Topics: (1) Vaccine development against gastrointestinal nematodes in cattle (Ostertagia ostertagi, Cooperia oncophora) (2) The epidemiology and control of cattle nematodes (3) Molecular epidemiology and zoonotic transmission of Giardia, (3) Treatment and control of Psoroptes ovis in cattle (4) Social sciences in parasite control. • Supervision of PhD students (promotor of 15 PhD theses) • Author or co-author of 156 scientific papers, of which 146 in peer reviewed international journals cited in the Science Citation Index • Editor of one book (2 editions)

Teaching experience in Veterinary Parasitology • Parasitology (3rd year Bachelor of veterinary medicine, tutorials and practicals) • Parasitic diseases (1st year Master of veterinary medicine, tutorials and wet lab diagnosis of parasitic diseases) • Lectures for post-graduate courses and for veterinary practitioners. Other relevant working experience • Representative of assistants in the board of directors of Ghent University (1999-2001) and representative of lecturers and senior lecturers in the board of the faculty of veterinary medicine and in several faculty committees (2004-2010), secretary of the board of the faculty of veterinary medicine (2011-). • Referee for scientific journals: Veterinary Parasitology (member of scientific advisory board), Journal of Helminthology (member of scientific advisory board), Frontiers in Veterinary Science (associated editor), PLoS One, Parasites & Vectors, International Journal for Parasitology, Vaccine, Molecular and Biochemical Parasitology, Veterinary Research, Veterinary Record, Helminthologia, Vlaams Diergeneeskundig Tijdschrift, Tijdschrift voor Diergeneeskunde • Referee for international research grants: The Wellcome Trust (UK); Biotechnology and Biological Sciences Research Council (UK), SANPAD (The Netherlands) SRTP Proposal Naples page 20 of 105

• Jury member (internal and external) for 21 PhD defences.

Memberships: • Member of the Belgian Society for Parasitology and Protistology (1992 - )  vice-president 2004-2005  president 2006-2007 • Member of the World Association for the Advancement of Veterinary Parasitology (1993 - ) • Member of the Belgian Council of Laboratory Animal Science (1997 - 2000) • Board member of the Belgian Center for Farmacotherapeutic Information (BCFI) (2006-) • Board member of the European Scientific Counsel Companion Animal Parasites (ESSCAP)- Benelux branch (2008-) • Diplomate of the European Veterinary Parasitology College (EVPC) (2004- )  Treasurer (2009-2015)  Vice-president (2015-2018) • President of the Livestock Helminth Research Alliance (2015-2016). • Member of the Scientific Committee (SC) of the STAR-IDAZ International Research Consortium on Animal Health (2016-)

Publication List (2012-2016)

● Sarre C., Claerebout E., Vercruysse J., Levecke B., Geldhof P., Pardon B., Alvinerie M., Sutra J.F., Geurden T. Doramectin resistance in Haemonchus contortus on an alpaca farm in Belgium. (2012) Veterinary Parasitology. 185(2-4). p.346-351. ● Grit G., Bénéré E. , Ehsan M. A., De Wilde N., Claerebout E., Vercruysse J., Maes L. Geurden T. Giardia duodenalis cyst survival in cattle slurry. (2012) Veterinary Parasitology. 184(2-4). p.330- 334. ● Sarre C., De Bleecker K., Deprez P., Levecke B., Charlier J., Vercruysse J., Claerebout E. Risk factors for Psoroptes ovis mange on Belgian Blue farms in Northern Belgium. (2012) Veterinary Parasitology. 190(1-2). p.216-221. ● Cochez C., Lempereur L., Madder M., Claerebout E., Simons L., De Wilde N., Linden A., Saegerman C., Heyman P., Losson B. Foci report on indigenous Dermacentor reticulatus populations in Belgium and a preliminary study of associated babesiosis pathogens. Medical And Veterinary Entomology. 26(3). p.355-358. ● Dreesen L., Rinaldi M., Chiers K., Li R., Geurden T., Van Den Broeck W., Goddeeris B., Vercruysse J., Claerebout E., Geldhof P. Microarray analysis of the intestinal host response in Giardia duodenalis assemblage E infected calves (2012) Plos One. 7(7). ● Geurden T., Vanderstichel R., Pohle H., Ehsan Md. A., von Samson-Himmelstjerna G., Morgan E.R., Camuset P., Capelli G., Vercruysse J., Claerebout E. A multicentre prevalence study in Europe on Giardia duodenalis in calves, with molecular identification and risk factor analysis (2012) Veterinary Parasitology. 190(3-4). p.383-390. ● De Graef J., Demeler J., Skuce P., Mitreva M., Von Samson-Himmelstjerna G., Vercruysse J., Claerebout E., Geldhof P.Gene expression analysis of ABC transporters in a resistant Cooperia SRTP Proposal Naples page 21 of 105

oncophora isolate following in vivo and in vitro exposure to macrocyclic lactones (2013) Parasitology. 140(4). p.499-508. ● Van Meulder F., Van Coppernolle S., Borloo J., Rinaldi M., Li R.W., Chiers K., Van Den Broeck W., Vercruysse J., Claerebout E., Geldhof P. Granule exocytosis of granulysin and granzyme B as a potential key mechanism in vaccine-induced immunity in cattle against the nematode Ostertagia ostertagi (2013) Infection And Immunity. 81(5). p.1798-1809. ● Obsomer V., Wirtgen M., Linden A., Claerebout E., Heyman P., Heylen D., Madder M., Maris J., Lebrun M., Tack W., et al. Spatial disaggregation of tick occurrence and ecology at a local scale as a preliminary step for spatial surveillance of tick-borne diseases: general framework and health implications in Belgium (2013) Parasites & Vectors. 6. ● Claerebout E., Losson B., Cochez C., Casaert S., Dalemans A., De Cat A., Madder M., Saegerman C., Heyman P., Lempereur L. Ticks and associated pathogens collected from dogs and cats in Belgium (2013) Parasites & Vectors. 6. ● Borloo J., De Graef J., Peelaers I., Nguyen D. L., Mitreva M., Bart D., Hokke C. H., Vercruysse J., Claerebout E., Geldhof P. In-depth proteomic and glycomic analysis of the adult-stage Cooperia oncophora excretome/secretome (2013) Journal Of Proteome Research. 12(9). p.3900-3911. ● Mihi B., Van Meulder F., Rinaldi M., Van Coppernolle S., Chiers K., Van Den Broeck W., Goddeeris B., Vercruysse J., Claerebout E., Geldhof P. Analysis of cell hyperplasia and parietal cell dysfunction induced by Ostertagia ostertagi infection (2013) Veterinary Research. 44. ● Grit G., Van Coppernolle S., Devriendt B., Geurden T., Dreesen L., Hope J., Vercruysse J., Cox E., Geldhof P., Claerebout E. Evaluation of cellular and humoral systemic immune response against Giardia duodenalis infection in cattle 2014) Veterinary Parasitology. 202(3-4). p.145-155. ● Grit G., Devriendt B., Van Coppernolle S., Geurden T., Hope J., Vercruysse J., Cox E., Geldhof P., Claerebout E. Giardia duodenalis stimulates partial maturation of bovine dendritic cells associated with altered cytokine secretion and induction of T-cell proliferation (2014) Parasite Immunology. 36(4). p.157-169. ● Mihi B., Van Meulder F., Vancoppernolle S., Rinaldi M., Chiers K., Van Den Broeck W., Goddeeris B., Vercruysse J., Claerebout E., Geldhof P. Analysis of the mucosal immune responses induced by single and trickle infections with the bovine abomasal nematode Ostertagia ostertagi (2014) Parasite Immunology. 36(4). p.150-156. ● Geurden T., van Doorn D., Claerebout E., Kooyman F., De Keersmaecker S., Vercruysse J., Besognet B., Vanimisetti B., Frangipane di Regalbono A., Beraldo P., et al. Decreased strongyle egg re-appearance period after treatment with ivermectin and moxidectin in horses in Belgium, Italy and The Netherlands (2014) Veterinary Parasitology. 204(3-4). p.291-296. ● Verschave S., Vercruysse J., Claerebout E., Rose H., Morgan E.R., Charlier J. The parasitic phase of Ostertagia ostertagi: quantification of the main life history traits through systematic review and meta-analysis (2014) International Journal For Parasitology. 44(14). p.1091-1104. ● Ehsan Md. A., Geurden T., Casaert S., Parvin S.M., Islam T.M., Ahmed U.M., Levecke B., Vercruysse J., Claerebout E. Assessment of zoonotic transmission of Giardia and Cryptosporidium between cattle and humans in rural villages in Bangladesh (2015) Plos One. 10(2). ● Ehsan Md. A., Geurden T., Casaert S., Paulussen J., De Coster L., Schoemaker T., Chalmers R., Grit G., Vercruysse J., Claerebout E. Occurrence and potential health risk of Cryptosporidium and Giardia in different water catchments in Belgium (2015) Environmental Monitoring And Assessment. 187(2). SRTP Proposal Naples page 22 of 105

● Vande Velde F., Claerebout E., Cauberghe V., Hudders L., Van Loo H., Vercruysse J., Charlier J. Diagnosis before treatment: identifying dairy farmers' determinants for the adoption of sustainable practices in gastrointestinal nematode control (2015) Veterinary Parasitology. 212(3-4). p.308-317. ● Van Meulder F., Ratman D., Van Coppernolle S., Borloo J., Li R.W., Chiers K., Van Den Broeck W., De Bosscher K., Claerebout E., Geldhof P. Analysis of the protective immune response following intramuscular vaccination of calves against the intestinal parasite Cooperia oncophora (2015) International Journal For Parasitology. 45(9-10). p.637-646. ● Charlier J., Vande Velde F., van der Voort M., Van Meensel J., Lauwers L., Cauberghe V., Vercruysse J., Claerebout E. ECONOHEALTH: placing helminth infections of livestock in an economic and social context (2015) Veterinary Parasitology. 212(1-2). p.62-67. ● Sarre C., González Hernández A., Van Coppernolle S., Grit G., Grauwet K., Van Meulder F., Chiers K., Van Den Broeck W., Geldhof P., Claerebout E. Comparative immune responses against Psoroptes ovis in two cattle breeds with different susceptibility to mange (2015) Veterinary Research. 46. ● Vlaminck J., Borloo J., Vercruysse J., Geldhof P., Claerebout E. Vaccination of calves against Cooperia oncophora with a double-domain activation-associated secreted protein reduces parasite egg output and pasture contamination (2015) International Journal For Parasitology. 45(4). p.209- 213. ● Verschave S., Rose H., Morgan E.R., Claerebout E., Vercruysse J., Charlier J. Modelling Cooperia oncophora: quantification of key parameters in the parasitic phase (2016) Veterinary Parasitology. 223. p.111-114. ● Verschave S., Charlier J., Rose H., Claerebout E., Morgan E. R. Cattle and nematodes under global chance: transmission models as an ally (2016) Trends In Parasitology. 32(9). p.724-738. ● Matthews J.B., Geldhof P., Tzelos T., Claerebout E. Progress in the development of subunit vaccines for gastrointestinal nematodes of ruminants (2016) Parasite Immunology . 38(12). p.744- 753. ● Charlier J., Ghebretinsae A. H., Meyns T., Czaplicki G., Vercruysse J., Claerebout E. Antibodies against Dictyocaulus viviparus major sperm protein in bulk tank milk: Association with clinical appearance, herd management and milk production (2016) Veterinary Parasitology. 232. ● Charlier J., Ghebretinsae A.H., Levecke B., Ducheyne E., Claerebout E. Vercruysse J. Climate- driven longitudinal trends in pasture-borne helminth infections of dairy cattle (2016) International Journal For Parasitology. 46 (13-14).

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Appendix 2: Description of Facilities, services and equipment for the Laboratory of Veterinary Parasitology, Department of Medical Veterinary Science, University of Parma, CV and publication list of Prof. Laura H. Kramer

Contact: Prof. Laura Helen Kramer, DVM, PhD, DipEVPC Department of Medical Veterinary Sciences, University of Parma, IT

The University of Parma Veterinary School currently accepts 53 students each year for the five-year course in Veterinary Medicine and there are approximately 400 students actively enrolled. There is one department (Veterinary Science) and 63 full-time professors and researchers. There are approximately 20 full-time laboratory technicians and 16 full-time administrative staff. There are three PhD programmes: Animal Production, Animal Health and Food Safety. The Small Animal Teaching Hospital at the University of Parma Veterinary School visits and treats approximately 3000 animals/year (data from 2012-2013), of which approximately 40% are dogs, 35% cats and the rest miscellaneous species (ferrets, reptiles, birds). Clinical rotations include general internal medicine, cardiology, dermatology, gastroenterology, neurology, diagnosis by imaging (radiography, US, endoscopy) and surgery. The Mobile Large Animal Clinic serves a geographical radius of approximately 100 kms in the province of Parma. Livestock include dairy cattle, beef cattle, swine and poultry. Clinical activities include screening for infectious diseases, obstetrics and surgery. Approximately 700 visits/year are made (data from 2012-2013). The Equine Fertility Clinic serves over 40 horses/year and is specialized AI and ET. The University of Parma Veterinary School also has an ongoing collaboration with several local slaughterhouses (bovine, equine, swine), guaranteeing adequate training in the post-mortem examination for food-borne parasitic zoonoses. Other facilities include a full-service library with over 1000 textbooks relating to Veterinary Medicine, General and Veterinary Parasitology and related disciplines. There is electronic access to over 80 journals relating to Veterinary Medicine, including all major journals of parasitology; necropsy facilities for large and small animals; histology archive with retrievable specimens. The University of Parma Veterinary School is accredited EAEVE, first level.

The Laboratory of Veterinary Parasitology at the University of Parma The Veterinary Parasitology Laboratory is equipped for all standard diagnostic procedures in Veterinary Parasitology. Diagnosis of common parasites is carried out on samples brought by general practitioners, as well as colleagues from Veterinary Teaching Hospital. Depending on the season and on the species considered, samples include: faeces, urine, blood, serum, skin and ear scrapings, tissue for histology.

The approximate number of diagnostic samples (feces, blood, urine, skin scrapings) ranges from 400-500/year (data from 2012-2013). The animal species considered include dog, cat, cattle, sheep, pig and, to a lesser extent, avian and reptile species. Procedures: -Flotation with different flotation solutions (NaCl, Zinc Sulphate, Sucrose…) SRTP Proposal Naples page 24 of 105

-Sedimentation -McMaster and FLOTAC enumeration procedures -Modified Knott test for microfilariae detection -Direct examination of skin/ear scrapings -Rapid ELISA kit for detection of parasite antigens in faeces -Baermann technique -Staining of fecal smears with modified Ziehl-Neelsen for Cryptosporidium spp. -ELISA for serology - immunocyto/histochemistry - Equipment for real-time and conventional PCR -Equipment for standard protein biochemistry techniques: protein preparations, SDS-PAGE, Western-Blot and 2-D electrophoresis.

Instruments: -2 stereo microscopes -2 optical microscopes -Leica RM2155Automatic microtome/histology line -BECKHAM refrigerated centrifuge PK 121R -OPTECH IB2FL inverted and fluoresence microscope -TKA Laminar flow hood -SANYO MCO-15AC CO2 incubator -ELISA washer/reader for serology

Parasite strains currently maintained Toxoplasma gondii Ixodes sp. cell lines Leishmania infantum

CV and publication list of Prof. Laura H. Kramer

Date/Place of Birth: September 22nd, 1960/ New York (USA) Nationality: USA/Italian

Education: 1978-1982 Bachelor of Science in Biology, Syracuse University, USA 1982-1984 Trinity College, Dublin Ireland (post-graduate course in Theology) 1984-1990 Degree in Veterinary Medicine from the University of Parma Veterinary School, Italy (graduated with honours) 1993-1996 PhD in Veterinary Pathology and Pathological Anatomy, University of Parma Veterinary School; thesis entitled “Feline Langerhans cells in healthy and in FIV+ cats.” 1996-1998 post-doctoral position in Veterinary Pathology and Pathological Anatomy, University of Parma Veterinary School; research project entitled “Langerhans cells in swine infected with Sarcoptes scabiei”

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Employment 1990-1993: Private practitioner for small animals at the “Vet Hospital” Veterinary Clinic, Reggio Emilia, Italy 1998-2004: Research Assistant in Veterinary Parasitology and Parasitic Diseases, University of Parma Veterinary School, Parma Italy 2004-present: Associate Professor of Veterinary Parasitology and Parasitic Diseases, University of Parma Veterinary School, via del Taglio 10, 43121 Parma Italy

Research experience in Veterinary Parasitology Prof. Laura Kramer began her experience in Veterinary Parasitology in 1996 as a study grant recipient for a project involving the local immune response to sarcoptic mange infection in swine. Her backround in veterinary pathology and her experience with immunohistochemical/ ultrastructural studies of dendritic cells in various animal species lead to an interest in host-parasite relationships and parasite immunology. Starting in 1998, collaboration with colleagues at the University of Milan on canine and feline heartworm disease lead to the participation in several research projects (national and European) on the epidemiology and immune response to filarial infection and the role of bacterial endosymbionts in the biology of filarial nematodes. This lead to the development and clinical evaluation of alternative adulticide protocols for canine heartworm disease. Other research interests and activity include the epidemiology of canine neosporosis and the role of local immune responses to Leishmania infantum infection in dogs.

Teaching experience in Veterinary Parasitology From 1998 to the present, Prof. Laura Kramer has been responsible for the teaching of Veterinary Parasitology and Parasitic Diseases at the University of Parma Veterinary School. This includes 90 hours of theory and 40 hours of wet labs, together with the guidance of those students who have chosen a project/topic in parasitology for their final year thesis. Prof. Laura Kramer currently spends all of her professional time in the teaching and research of Veterinary Parasitology at the University of Parma Veterinary School. She is also responsible for the management of the Parasitology Laboratory at the Veterinary School and is currently in charge of the PhD program in Veterinary Parasitology and the University of Parma.

GRANTS HELD: • Morris Animal Foundation (USA) – pilot study 2006 Canine dendritic cells in Leishmania infantum infection (30,000$) • MIUR, COFIN 2006-2009 Emerging microrganisms from Ixodidae ticks: isolation of Midichloria mitochondrii and in vitro infection of tick and mammal cell lines (190,000E) • MIUR-PRIN 2010-2011. Tick-drug interactions in Ixodes ricinus: in-vitro studies on pgp and Midichloria mitochondrii function in I. ricinus exposed to various drug regimes (120,000E)

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Memberships: • Società Italiana di Parassitologia (SOIPA) • World Association for the Advancement of Veterinary Parasitology (WAAVP) • European Dirofilaria Society (EDiS)

Publication List (2012 – 2016 * = corresponding authorship) • Morchón R, Carretón E, Grandi G, González-Miguel J, Montoya-Alonso JA, Simón F, Genchi C, Kramer LH*. Anti-Wolbachia Surface Protein antibodies are present in the urine of dogs naturally infected with Dirofilaria immitis with circulating microfilariae but not in dogs with occult infections. Vector Borne Zoonotic Dis. 2012 Jan;12(1):17-20 • Carretón E, Grandi G, Morchón R, Simón F, Passeri B, Cantoni AM, Kramer L*, Montoya-Alonso JA. Myocardial damage in dogs affected by heartworm disease (Dirofilaria immitis): Immunohistochemical study of cardiac myoglobin and troponin I in naturally infected dogs. Vet Parasitol. 2012 Oct 26;189(2-4):390-3. • Carretón E, González-Miguel J, Montoya-Alonso JA, Morchón R, Simón F, Passeri B, Cantoni AM, Kramer L.* D-dimer deposits in lungs and kidneys suggest its use as a marker in the clinical workup of dogs with heartworm (Dirofilaria immitis) disease. Vet Parasitol. 2012 Aug 20. [Epub ahead of print] PubMed PMID: 22963711. • García-Guasch, L., Caro-Vadillo, A., Manubens-Grau, J., Carretón, E., Morchón, R., Simón, F., Kramer, L.H., Montoya-Alonso, J.A. Is Wolbachia participating in the bronchial reactivity of cats with heartworm associated respiratory disease? (2013) Veterinary Parasitology, 196 (1-2), pp. 130- 135. • Carretón, E., González-Miguel, J., Montoya-Alonso, J.A., Morchón, R., Simón, F., Passeri, B., Cantoni, A.M., Kramer, L.* D-dimer deposits in lungs and kidneys suggest its use as a marker in the clinical workup of dogs with heartworm (Dirofilaria mmitis) disease (2013) Veterinary Parasitology, 191 (1-2), pp. 182-186. • González-Miguel, J., Morchón, R., Gussoni, S., Bossetti, E., Hormaeche, M., Kramer, L.H., Simón, F. Immunoproteomic approach for identification of Ascaris suum proteins recognized by pigs with porcine ascariasis (2014) Veterinary Parasitology, 203 (3-4), pp. 343-348. • Kramer, L., Genchi, C. Where are we with Wolbachia and doxycycline: An in-depth review of the current state of our knowledge (2014) Veterinary Parasitology, . Article in Press. • Mavropoulou, A., Gnudi, G., Grandi, G., Volta, A., Kramer, L.H.*, Quintavalla, C. Clinical assessment of post-adulticide complications in Dirofilaria immitis-naturally infected dogs treated with doxycycline and ivermectin (2014) Veterinary Parasitology, 205 (1-2), pp. 211-215. • Morchón, R., González-Miguel, J., Carretón, E., Kramer, L.H., Valero, L., Montoya-Alonso, J.A., Simón, F., Siles-Lucas, M. Proteomic analysis of the somatic and surface compartments from Dirofilaria immitis adult worms (2014) Veterinary Parasitology, 203 (1-2), pp. 144-152. • Passeri, B., Vismarra, A., Cricri, G., Bazzocchi, C., Kramer, L.*, Bacci, C. The adulticide effect of a combination of doxycycline and ivermectin in Dirofilaria immitis-experimentally infected dogs is associated with reduction in local T regulatory cell populations (2014) Veterinary Parasitology, 205 (1-2), pp. 208-210. • Cafarchia, C., Porretta, D., Mastrantonio, V., Epis, S., Sassera, D., Iatta, R., Immediato, D., Ramos, R.A., Lia, R.P., Dantas-Torres, F., Kramer, L., Urbanelli, S., Otranto, D. Potential role of ATP- SRTP Proposal Naples page 27 of 105

binding cassette transporters against acaricides in the brown dog tick Rhipicephalus sanguineus sensu lato (2015). Medical and Veterinary Entomology, 29(1), pp. 88-93. • Menozzi, A., Bertini, S., Turin, L., Serventi, P., Kramer, L.*, Bazzocchi, C. Doxycycline levels and anti-Wolbachia antibodies in sera from dogs experimentally infected with Dirofilaria immitis and treated with a combination of ivermectin/doxycycline (2015). Veterinary Parasitology, 209(3-4), pp.281-4. • Vismarra, A., Mangia, C., Passeri, B., Brundu, D., Masala, G., Ledda, S., Mariconti, M., Brindani, F., Kramer, L.*, Bacci, C. Immuno-histochemical study of ovine cystic echinococcosis (Echinococcus granulosus) shows predominant T cell infiltration in established cysts (2015). Veterinary Parasitology, 209(3-4), pp. 285-8. • Bacci, C., Vismarra, A., Mangia, C., Bonardi, S., Bruini, I., Genchi, M., Kramer, L.*, Brindani, F. Detection of Toxoplasma gondii in free-range, organic pigs in Italy using serological and molecular methods (2015), International Journal of Food Microbiology, 202, pp.54-6. • Mangia, C., Vismarra, A., Kramer, L.*, Bell-Sakyi, L., Porretta, D., Otranto, D., Epis, S., Grandi, G. Evaluation of the in vitro expression of ATP binding-cassette (ABC) proteins in an Ixodes ricinus cell line exposed to ivermectin (2016). Parasites & Vectors, 9:215. • Ciucă, L., Genchi, M., Kramer, L.*, Mangia, C., Miron, L.D., Del Prete L., Maurelli, M.P., Cringoli, G., Rinaldi, L. Heat treatment of serum samples from stray dogs naturally exposed to Dirofilaria immitis and Dirofilaria repens in Romania (2016). Veterinary Parasitology, 225, pp. 81- 5. • Vismarra, A., Mangia, C., Barilli, E., Brindani, F., Bacci, C., Kramer, L. Meat Juice Serology for Toxoplasma gondii Infection in Chickens (2016). Ital J Food Saf., 5(1), pp.5586.

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Appendix 3: Description of Facilities, services of the Clinic for companion animals “Città di Pavia”, Pavia Italy; CV and publication list of Dr. Luigi Venco

Contact: Dr. Luigi Venco, DVM, dipEVPC Veterinary Hospital “Città di Pavia”, Pavia, Italia

Clinic for companion animals “Città di Pavia”, Pavia Italy Veterinary Hospital for companion animals “Città di Pavia”is a fully equipped Hospital open 24 hours a day; 5 partners 12 veterinary, 2 technicians and 2 front desk people work there. The Hospital is equipped by 5 visiting rooms. 2 operating theaters , abdominal and cardiac sonography, radiology, fluoroscopy, CT scan and an internal laboratory for haematology, biochemistry, cytology and parasitology (coprological standard examinations, skin scrapings, identification of ectoparasites, rapid ELISA tests, modified Knott’s test, hematological and cytological exams of parasitic interest). The head of the laboratory is Dr. Walter Bertazzolo (ECVCP diplomate). An average of 50 clinical cases are managed every day. The Hospital is a referral place for clinical parasitology and cardiology and is the only hospital in Europe where surgical heartworm removal via jagular vein (Ishihara technique’s) is performed (an average of 2 cases a week) by Dr. L. Venco (EVPC diplomate), which is also responsible for diagnosis and management of most parasitological clinical cases.

CV and publication list of Dr. Luigi Venco

Date/Place of Birth: April 1st, 1961/ Milan (Italy) Nationality: Italian

Education: 1975-1980 High School Liceo Classico Pavia, Italy 1980-1987 Degree in Veterinary Medicine from the University of Milan,Veterinary School, Italy Post-graduate training 1988-1990 Board “Small animal practice” from the University of Milan, Veterinary School, Italy 1992-1993 “Veterinary cardiology course” from the University of Turin, Veterinary School, Italy 2006 “de facto” European Veterinary Parasitology College Diplomate

Employment 1988 -1995: Private practitioner at the mixed large/small animal practice Clinica Veterinaria Croce Azzurra (via Pirandello 15, 27045 Casteggio, PV. Italy ) 1991-present: Private practitioner at the small animal practice Clinica Veterinaria Città di Pavia (viale Cremona 179, 27100 Pavia. Italy) 2012-present Parasitology consultant of Laboratorio analisi veterinarie “La Vallonea” Italy

Research experience in Veterinary Parasitology June-July 1993 Project title: Clinical efficacy of surgical heartworm removal in dogs. Veterinary Teaching Hospital. School of Veterinary Medicine. University of Gifu, Gifu, Japan. May-November 1997 SRTP Proposal Naples page 29 of 105

Project title: Experimentally induced heartworm disease in dogs, cats and ferrets. Department of Microbiology and Parasitology, School of Veterinary Medicine. University of Georgia. Athens, GA (USA) October-November 1998 Project title: The effect of ivermectin on anti-Dirofilaria immitis antibodies in cats receiving experimental infection with D. immitis Heska Corporation / Veterinary Teaching Hospital. Faculty of Veterinary Medicine, Colorado State University Fort Collins CO (USA)

Teaching experience in Veterinary Parasitology October 1997 Visiting Professor at the Faculty of Veterinary Medicine, Department of Small Animal Medicine. University of Georgia. Athens (GA) USA. Seminar title. “The use of echocardiography in the canine and feline heartworm disease” October 1999 - May 2012 Visiting Professor at the Faculty of Veterinary Medicine, University of Milan, Italy. Course title: “Parasitology and Parasitic diseases of small animals”. October 2002 Invited Professor at “AVULP” Associazione Veterinari Umbra Liberi Professionisti” Perugia Italy Seminar title “ Canine and Feline Heartworm Disease : Biology – Pathogenesis – Clinical features – Radiology – Echocardiography and Elettrocardiography – Supportive treatments – Adulcidal treatments – Surgical treatment – Chemoprophylaxis “

Memberships: • Società Italiana di Parassitologia (SOIPA) • World Association for the Advancement of Veterinary Parasitology (WAAVP) • American Heartworm Society • Vice President of European Dirofilaria and Angiostrongylus Society (EDiS)

Publication List (2012 – 2016 )

• Traversa D, Di Cesare A, Meloni S, Frangipane di Regalbono A, Milillo P, Pampurini F, Venco L. Canine angiostrongylosis in Italy: occurrence of Angiostrongylus vasorum in dogs with compatible clinical pictures. Parasitol Res. 2013 Jul;112(7):2473-80. • Albanese F, Abramo F, Braglia C, Caporali C, Venco L, Vercelli A, Ghibaudo G, Leone F, Carrani F, Giannelli A, Otranto D. Nodular lesions due to infestation by Dirofilaria repens in dogs from Italy. Vet Dermatol. 2013 Apr;24(2):255-e56. • Venco L, Bertazzolo W, Giordano G, Paltrinieri S. Evaluation of C-reactive protein as a clinical biomarker in naturally heartworm-infected dogs: A field study. Vet Parasitol. 2014 Sep 1. pii: S0304-4017(14)00471-3. doi: 10.1016/j.vetpar.2014.08.018. • Amati M, Venco L, Roccabianca P, Santagostino SF, Bertazzolo W. Pericardial lymphoma in seven cats. J Feline Med Surg. 2014 Jun;16(6):507-12. • Venco L, Mihaylova L, Boon JA. Right Pulmonary Artery Distensibility Index (RPAD Index). A field study of an echocardiographic method to detect early development of pulmonary hypertension SRTP Proposal Naples page 30 of 105

and its severity even in the absence of regurgitant jets for Doppler evaluation in heartworm-infected dogs. Vet Parasitol. 2014 Nov 15;206(1-2):60-6. • Cavaliere L, Romito G, Domenech O, Venco L. Heartworm Removal Guided by Transesophageal Echocardiography in a Dog with Naturally Acquired Caval Syndrome. J Am Anim Hosp Assoc. 2016 Nov 14. • Manzocchi S, Venco L, Di Cesare A. What is your diagnosis? Squash preparation from the lung of a hedgehog. Vet Clin Pathol. 2016 Dec;45(4):715-716. • Lacava G, Zini E, Marchesotti F, Domenech O, Romano F, Manzocchi S, Venco L, Auriemma E. Computed tomography, radiology and echocardiography in cats naturally infected with Aelurostrongylus abstrusus. J Feline Med Surg. 2016 Mar 9. pii: 1098612X16636419. [Epub ahead of print] • Venco L, Marchesotti F, Manzocchi S. Feline heartworm disease: A'Rubik's-cube-like' diagnostic and therapeutic challenge. J Vet Cardiol. 2015 Dec;17 Suppl 1:S190-201. • Di Cesare A, Traversa D, Manzocchi S, Meloni S, Grillotti E, Auriemma E, Pampurini F, Garofani C, Ibba F, Venco L. Elusive Angiostrongylus vasorum infections. Parasit Vectors. 2015 Aug 27;8:438. • Venco L, Bertazzolo W, Giordano G, Paltrinieri S. Evaluation of C-reactive protein as a clinical biomarker in naturally heartworm-infected dogs: a field study. Vet Parasitol. 2014 Nov 15;206(1- 2):48-54.

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Appendix 4: Description of Facilities, services and equipment of the Institute of Parasitology, Vetsuisse Faculty, University of Zurich, Switzerland; CV and publication list of Prof. Peter Deplazes

Contact: Prof. Peter Deplazes DVM, PhD, DipEVPC

Institute of Parasitology, Vetsuisse Faculty, University of Zurich, Switzerland

Scientific visitors will be guided by 3 EVPC Diplomats: -Prof. Dr. med. vet. P. Deplazes, -PD Dr. med. vet. H. Hertzberg -Dr. med. vet. M. Schnyder and by : - Prof. Dr. phil. nat. A. Hehl (section Molecular Parasitology) -Prof. sc. nat. ETH A. Mathis (section Arachno-Entomology) -Dr. phil. F. Grimm (diagnostic centre parasitology).

The Institutes of Parasitology in Berne and Zurich belong to the same faculty, the Vetsuisse Faculty. The Institute of Parasitology in Zurich (IPZ) was founded in 1968 as a dual institution of the veterinary and medical faculty. The IPZ is appointed by the federal veterinary office as the ”National Reference Centre” for echinococcosis and cryptosporidiosis.

Since 2007, this function has been expanded to include the field of vector entomology. In the field of Parasitic Zoonoses”, the Institute maintains collaborations with research groups in a number of other countries (especially UK, F, D, I, USA, Australia, DK, Kyrgyzstan, China and Lithuania). A large part of research is supported by a variety of national (NF, BLV, University of Zurich) and international funding organizations (BBW, NIH, INTAS, FAO, COST), as well as private companies (Novartis, Bayer) and foundations (3R, Gebert-Rüf, Messerli, Mercier, BaltNet). Scientists visiting from abroad have made significant contributions to the initiation and strengthening of important international co-operation.

In particular, the staff consists of 4 EVPC diplomates, 3 residents of EVPC, 6 biologists and 11 lab technicians, all of which are familiar with laboratory management and safety policies through information sessions and periodical evaluation. Additionally, a variable number (6-12) of post-graduate veterinarians and biologists and 3 animal care takers are present at the IPZ. Approximately 30 employees and 10 doctoral students are regularly involved with research (basic and problem oriented research in parasitology), teaching (for the university of Zurich) and diagnostic services for veterinary and human Parasitology.

The institute has a library, 3 principal parasitological units and animal facilities, which are approved to meet the Biological Safety and Health standards set by European legislation (L2). Each clinical trial performed by the Institute of Parasitology which includes animals is evaluated by the cantonal veterinary office and a local Ethical Committee in order to conform to the legislation SRTP Proposal Naples page 32 of 105

on clinical trials.

Teaching activities by members of the institute reaches veterinary, medical and biology students at all levels. In addition, the IPZ is involved in postgraduate teaching, in which parasitology seminars, diagnostic case reports and a colloquium on current aspects of research in parasitology are on offer. The diagnostic services (ISO/IEC accreditation 17025) provided by the IPZ in medical and veterinary parasitology are a key source of experience and information for teaching and research.

Case load The total number of annual diagnostic tests is around 25,000 and Include samples from dogs, cats, cattle, horses, swine and poultry.

The Zürich institute will provide expertise and training (1-4 weeks as required) in the following areas: -Morphological, immunological and molecular diagnostic techniques; -Development and validation of diagnostic tests and strategies; -In vitro and in vivo maintenance of parasite strains; -Epidemiology and ecology of helminths, specific training in vector-entomology.

CV and publication list of Prof. Peter Deplazes Date/Place of Birth: August 20th 1956/Sumvitg (GR), Switzerland Nationality: Swiss

Employment history including current position

1979 – 1984: Studies of Veterinary Medicine at the University of Zurich 1988 Dissertation: Institute of Parasitology, University of Zurich (Prof. Dr. Dr. h.c. J. Eckert) July 1998, Title: "Experimental studies on the reproduction biology of Taenia hydatigena in dogs" 1988 – 1990: Assistant at the Institute of Parasitology, University of Zurich, Department of Veterinary Serology and Immunology 1991: Grant of the Swiss National Foundation: Research stay at the Institute for Molecular Genetics and Animal Disease, School of Veterinary Studies, Murdoch University, Murdoch (Western Australia) 1997: Habilitation: Venia legendi on "Parasitology", University of Zurich, February 1997, Title: "Immunology and immune- and molecular diagnosis of Echinococcus and Taenia infections in carnivores” 1999: Visiting Professor, The Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Copenhagen, Denmark 2000: Full professor of Parasitology, Director of the Institute of Parasitology and Head of Laboratory for Zoonoses, Institute of Parasitology, University of Zurich 2007: Sabbatical at the Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine University of California, Davis, U.S.A. (in the group of Professor Patricia A Conrad) SRTP Proposal Naples page 33 of 105

2016: Sabbatical at the Bhutan National Centre for Animal Health in Serbithang, Thimphu, Bhutan

Research experience in Veterinary Parasitology Since 2000 I am the Director of the Institute of Parasitology and Head of Laboratory for Zoonoses, Institute of Parasitology, University of Zurich Supervision of junior researchers at graduate and postgraduate level Organisations of following confereces: The Paratrop 2014 Congress, as a joint meeting of Parasitology and Tropical Medicine at the Irchel- Campus of the University of Zurich, July 16 - 19, 2014 Echinococcus 2014 Congress and international Meeting in Vilnius (Lithuania) October 8 – 9, 2014.

Teaching experience in Veterinary Parasitology I am responsible for teaching of parasitology at the Vetsuisse Faculty and at the Medical Faculty of the University of Zurich.

Major scientific achievements: 1) Pet transmitted zoonoses is a research focus of our group. In the past toxocarosis and echinococcosis have been the main focus (Deplazes et al., 2011). More recently, we are intensively focusing on the transmission of Toxoplasma gondii in cats and the development of a vaccine. 2) Echinococcus transmission and control: this research is based on experimental studies with a variety of hosts. Especially the work with carnivores, pigs and wild rodent species are of value for further risk assessments and modelling. Control strategies have been evaluated by my group in the past, especially for urban areas. Comprehensive reviews documenting our scientific achievements are (Hegglin and Deplazes, 2013, Hegglin et al., 2015). Alveolar Echinococcosis is a neglected but serious and often lethal disease if not treated. In a “One Health” concept our group was closely collaborating with the medical area focusing on the validation of diagnostic strategies and chemotherapy. This efforts are documented is several publications (Deplazes et al., 2015, Gottstein et al., 2015, Ammann et al., 2015). Further information: http://www.research- projects.uzh.ch/p10435.htm Development of diagnostic tests and diagnostic strategies (translational research) 1) One major focus in the last years was the development of serological tests for Angiostrongylus vasorum, an important canine parasite (see Schnyder et al. 2011a, 2011b, 2012, 2013, 2014, Schucan et al. 2012). This development resulted in a commercialised rapid diagnostic test (IDEXX). Further information: http://www.research-projects.uzh.ch/p10434.htm 2) Since 2000 we are developing tools for the diagnosis of Echinococcosis in definitive, intermediate and dead end hosts. Several molecular tests have been developed for specific diagnosis of the parasite stages in animals and humans including from environmental samples. In this field we are one of the leading groups (see Conraths and Deplazes, 2015) and in the last years many international collaborations were performed with the aim of exchanging technical and methodological knowledge (collaborations with groups in Lithuania http://www.research- projects.uzh.ch/p4982.htm, Kosovo http://www.research-projects.uzh.ch/p16369.htm, Kyrgiztan and Bhutan). Further information http://www.research-projects.uzh.ch/p10436.htm

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Memberships: Founding member of European Companion Animal Parasites (ESCCAP) Founding member of European Veterinary Parasitology College (EVPC) Member of the Piekarski Price Commission of DGP Member of the advisory board of the Karl Enigk Member of the Swiss Society for Veterinary Diagnosticians Member of the German Society for Parasitology (DGP) Member of the World Association for the Advancement of Veterinary Parasitology (WAAVP) and Member of the Swiss Society for Tropical Medicine and Parasitology (SGTP) Member of the Vetsuisse Research Committee Ad hoc reviewer for science foundations (e.g. SNF, , DFG, Humbold Stiftung) and various journals in the fields of, parasitology, infectious diseases, and veterinary medecine Associate Editor, of Infection, Vet. Parasitology (until 2016) and International Journal of Parasitology, Parasites and Wildlife

Publication List (2012-2016)

• Fiechter, R., Deplazes, P., Schnyder, M.: Control of Giardia infections with ronidazole and intensive hygienemanagement in a dog kennel. Vet. Parasitol. 187, 93 – 98 (2012). • Schucan, A., Schnyder, M., Tanner, I., Barutzki, D., Traversa, D., Deplazes, P.: Detection of specific antibodies in dogs infected with Angiostrongylus vasorum. Vet. Parasitol. 185, 216 – 224 (2012). • Barnes, T.S., Deplazes, P., Gottstein, B., Jenkins, D.J., Mathis, A., Siles-Lucas, M., Torgerson, P.R., Ziadinov, I., Heath, D.D.: Challenges for diagnosis and control of cystic hydatid disease. Acta Trop. 123, 1 – 7 (2012). • Schweiger, A., Grimm, F., Tanner, I., Müllhaupt, B., Bertogg, K., Müller, N., Deplazes, P.: Serological diagnosis of echinococcosis: the diagnostic potential of native antigens. Infection 40, 139 – 152 (2012). • Matos, J.M., Schnyder, M., Bektas, R., Makara, M., Kutter, A., Jenni, S., Deplazes, P., Glaus, T.: Recruitment of arteriovenous pulmonary shunts may attenuate the development of pulmonary hypertension in dogs experimentally infected with Angiostrongylus vasorum. J. Vet. Cardiol. 14, 313 – 322 (2012). • Knapp, J., Staebler, S., Bart, J.M., Stien, A., Yoccoz, N.G., Drögemüller, c., Gottstein, B., Deplazes, P.: Echinococcus multilocularis in Svalbard, Norway: Microsatellite genotyping to investigate the origin of a highly focal contamination. Infect. Genet. Evol. 12, 1270 – 1274 (2012). • Gasser, R.B., Jabbar, A., Mohandas, N., Schnyder, M., Deplazes, P., Littlewood, D.T.J., Jex, A.R.: Mitochondrial genome of Angiostrongylus vasorum: Comparison with congeners and implications for studying the population genetics and epidemiology of this parasite. Infect. Genet. Evol. 12, 1884 – 1891 (2012). • Barth, T.F.E., Herrmann, T.S., Tappe, D., Stark, L., Grüner, B., Buttenschoen, K., Hillenbrand, A., Juchems, M., Henne-Bruns, D., Kern, P., Seitz, H.M., Möller, P., Rausch, R.L., Kern, P., Deplazes, P.: Sensitive and Specific Immunohistochemical Diagnosis of Human Alveolar Echinococcosis with the Monoclonal Antibody Em2g11. Plos. Neglect. Trop. D. 6(10): e1877. doi :10.1371/journal- pntd.0001877 (2012). SRTP Proposal Naples page 35 of 105

• Lesser, M., Braun, U., Deplazes, P., Gottstein, B., HIilbe, M., Basso, W.: Erste Fälle von Besnoitiose bei Rindern in der Schweiz. Schweiz. Arch. Tierheilkd. 154, 469 – 474 (2012). • Schnyder, M., Deplazes, P.: Cross-reactions of sera from dogs infected with Angiostrongylus vasorum in commercially available Dirofilaria immitis test kits. Parasites Vectors 5: 258 (2012). • Takumi, K., Hegglin, D., Deplazes, P., Gottstein, B., Teunis, P., Van der Giessen, J.: Mapping the increasing risk of human alveolar echinococcosis in Limburg, The Netherlands. Epidemiol. Infect. 140, 867 – 871 (2012). • Guardone, L., Schnyder, M., Macchioni, F., Deplazes, P., Magi, M.: Serological detection of circulating Angiostrongylus vasorum antigen and specific antibodies in dogs from central and northern Italy. Vet. Parasitol. 192, 192 – 198 (2013). • Boubaker, G., Macchiaroli, N., Prada, L., Cucher, M.A., Rosenzvit, M.C., Ziadinov, I., Deplazes, P., Saarma, U., Babba, H., Gottstein, B., Spiliotis, M.: A multiplex PCR for the simultaneous detection and genotyping of the Echinococcus granulosus complex. Plos. Neglect. Trop. D. 7, Epub e2017. doi: 10.1371/journal.pntd.0002017 (2013). • Minbaeva, G., Schweiger, A., Bodosheva, A., Kuttubaev, O., Hehl, A.B., Tanner, I., Ziadinov, I., Torgerson, P.R., Deplazes, P.: Toxoplasma gondii Infection in Kyrgyzstan: Seroprevalence, Risk Factor Analysis, and Estimate of Congenital and AIDS-Related Toxoplasmosis. Plos. Neglect. Trop. D. 7, Epub e2043. doi: 10.1371/journal.pntd.0002043 (2013). • Magnis, J., Lorentz, S., Guardone, L., Grimm, F., Magi, M., Naucke, T.J., Deplazes, P.: Morphometric analyses of canine blood microfilariae isolated by the Knott's test enables Dirofilaria immitis and D. repens species-specific and Acanthocheilonema (syn. Dipetalonema) genus-specific diagnosis. Parasite Vector 6:48 Epub (2013). • Tsai, I.J., Zarowiecki, M., Holroyd, N., Garciarrubio, A., Sanchez-Flores, A., Brooks, K.L., Tracey, A., Bobes, R.J., Fragoso, G., Sciutto, E., Aslett, M., Beasley, H., Bennett, H.M., Cai, J., Camicia, F., Clark, R., Cucher, M., De Silva, N., Day, T.A., Deplazes, P., Estrada, K., Fernández, C., Holland, P.W., Hou, J., Hu, S., Huckvale, T., Hung, S.S., Kamenetzky, L., Keane, J.A., Kiss, F., Koziol, U., Lambert, O., Liu, K., Luo, X., Luo, Y., Macchiaroli, N., Nichol, S., Paps, J., Parkinson, J., Pouchkina-Stantcheva, N., Riddiford, N., Rosenzvit, M., Salinas, G., Wasmuth, J.D., Zamanian, M., Zheng, Y.; The Genome Consortium, Garciarrubio, A., Bobes, R.J., Fragoso, G., Sánchez-Flores, A., Estrada, K., Cevallos, M.A., Morett, E., González, V., Portillo, T., Ochoa- Leyva, A., José, M.V., Sciutto, E., Landa, A., Jiménez, L., Valdés, V., Carrero, J.C., Larralde, C., Morales-Montor, J., Limón-Lason, J., Soberón, X., Laclette, J.P., Cai, X., Soberón, X., Olson, P.D., Laclette, J.P., Brehm, K., Berriman, M.: The genomes of four tapeworm species reveal adaptations to . Nature, Epub 10.1038/nature12031 (2013). • Hegglin, D., Deplazes, P.: Control of Echinococcus multilocularis: Strategies, feasibility and cost- benefit analyses. Int. J. Parasitol. 43, 327 – 337 (2013). • Glor, S.B., Edelhofer, R., Grimm, F., Deplazes, P., Basso, W.: Evaluation of a commercial ELISA kit for detection of antibodies against Toxoplasma gondii in serum, plasma and meat juice from experimentally and naturally infected sheep. Parasites Vectors 6:85 (2013). • Comte, S., Raton, V., Raoul, F., Hegglin, D., Giraudoux, P., Deplazes, P., Favier, S., Gottschek, D., Umhang, G., Boué, F., Combes, B. Fox baiting against Echinococcus multilocularis: Contrasted achievements among two medium size cities. Prev. Vet. Med. 111, 147 - 155 (2013). • Basso, W., Hartnack, S., Pardini, L., Maksimov, P., Koudela, B., Venturini, M.C., Schares, G., Sidler, X., Lewis, F.I., Deplazes, P.: Assessment of diagnostic accuracy of a commercial ELISA for SRTP Proposal Naples page 36 of 105

the detection of Toxoplasma gondii infection in pigs compared with IFAT, TgSAG1-ELISA and Western blot, using a Bayesian latent class approach. Int. J. Parasitol. 43, 565 – 570 (2013).. • Schnyder, M., Schaper, R., Pantchev, N., Kowalska, D., Szwedko, A., Deplazes, P.: Serological Detection of Circulating Angiostrongylus vasorum Antigen- and Parasite-Specific Antibodies in Dogs from Poland. Parasitol. Res. 112, 109 – 117 (2013a). • Usubalieva, J., Minbaeva, G., Ziadinov, I., Deplazes, P., Torgerson, P.R.: Human Alveolar Echinococcosis in Kyrgyzstan. Emerg. Infect. Dis. 19, 1095 – 1097 (2013). • Eichenberger, R.M., Lewis, F., Gabriël, S., Dorny, P., Torgerson, P.R., Deplazes, P.: Multi-test analysis and model-based estimation of the prevalence of cysticercus infection in naturally infected dairy cows in the absence of a ‘gold standard’ reference test. Int. J. Parasitol. 43, 853-859 • Schnyder, M., Schaper, R., Bilbrough, G., Morgan, E.R., Deplazes, P.: Seroepidemiological survey for canine angiostrongylosis in dogs from Germany and the UK using combined detection of Angiostrongylus vasorum antigen and specific antibodies. Parasitology 140, 1442 – 1450 (2013). • Juránková, J., Basso, W., Neumayerová, H., Baláz, V., Jánová, E., Sidler, X., Deplazes, P., Koudela, B.: Brain is the predilection site of Toxoplasma gondii in experimentally inoculated pigs as revealed by magnetic capture and real-time PCR. Food Microbiol. 38, 167 – 170 (2013). • Helm, M., Müller, W., Schaarschmidt, D., Grimm, F., Deplazes, P.: Allopurinol-Therapie bei importierten Hunden mit Leishmaniose ausserhalb des Endemiegebietes. Schweiz. Arch. Tierh. 155(10), 559 – 567 (2013). • Ansell, B.R.E., Schnyder, M., Deplazes, P., Korhonen, P.K., Young, N.D., Hall, R.S., Mangiola, S., Boag, P.R., Hofmann, A., Sternberg, P.W., Jex, A.R., Gasser, R.B.: Insights into the immuno- molecular biology of Angiostrongylus vasorum through transcriptomics – Prospects for new interventions. Biotechnol. Adv. 31, 1486 – 1500 (2013). • Guerra, D., Armua-Fernandez, M.T., Silva, M., Bravo, I., Santos, N., Deplazes, P., Madeira de Carvalho, L.M. : Taeniid species of the Iberian wolf (Canis lupus signatus) in Portugal with special focus on Echinococcus spp. Int. J. Parasitol.: Parasites and Wildlife 2, 50 – 53 (2013). • Dennler, M., Bass, D.A., Gutierrez-Crespo, B., Schnyder, M., Guscetti, F., Di Cesare, A., Deplazes, P., Kircher, P.R., Glaus, T.M.: Thoracic computed tomography, angiographic computed tomography, and pahtology findings in six cats experimentally infected with Aelurostrongylus abstrusus. Vet. Radiol. Ultrasound. 54(5), 459 – 469 (2013). . • Basso, W., Lesser, M., Grimm, F., Hilbe, M., Sydler, T., Trösch, L., Ochs, H., Braun, U., Deplazes, P.: Bovine besnoitiosis in Switzerland: Imported cases and local transmission. Vet. Parasitol. 198(3- 4), 265-273 (2013). • Guardone, L, Deplazes, P., Macchioni, F., Magi, M., Mathis, A.: Ribosomal and mitochondrial DNA analysis of Trichuridae nematodes of carnivores and small mammals. Vet. Parasitol. 197(1-2), 364 – 369 (2013). • Basso, W., Rütten, M., Deplazes, P., Grimm, F.: Generalized Taenia crassiceps cysticercosis in a chinchilla (Chinchilla lanigera). Vet. Parasitol. 199(1-2), 116 – 120 (2014). • Basso, W., Spänhauer, Z., Arnold, S., Deplazes, P.: Capillaria plica (syn. Pearsonema plica) infection in a dog with chronic pollakiuria: Challenges in the diagnosis and treatment. Parasitol. Int. 63, 140 – 142 (2014). • Armua-Fernandez, M.T., Castro, O.F., Crampet, A., Bartzabal, A., Hofmann-Lehmann, R., Grimm, F., Deplazes, P.: First case of peritoneal cystic echinococcosis in a domestic cat caused by SRTP Proposal Naples page 37 of 105

Echinococcus granulosus sensu stricto (genotype 1) associated to feline immunodeficiency virus infection. Parasitol. Int. 63(2), 300 – 302 (2014). • Schnyder, M., Di Cesare, A., Basso, W., Guscetti, F., Riond, B., Glaus, T., Crisi, P., Deplazes, P.: Clinical, laboratory and pathological findings in cats experimentally infected with Aelurostrongylus abstrusus. Parasitol. Res. 113, 1425 – 1433 (2014). • Lewis, F.I., Otero-Abad, B., Hegglin, D., Deplazes, P., Torgerson, P.R.: Dynamics of the Force of Infection: Insights from Echinococcus multilocularis Infection in Foxes. Plos. Negl. Trop. Dis. 8(3), e2731 (2014). • Schnyder, M., Stebler, K., Naucke, T.J., Lorentz, S., Deplazes, P.: Evaluation of a rapid device for serological in-clinic diagnosis of canine angiostrongylosis. Parasit. Vectors. 7:72 (2014). • Motta, B., Nägeli, F., Nägeli, C., Solari-Basano, F., Schiessl, B., Deplazes, P., Schnyder, M.: Epidemiology of the eye worm Thelazia callipaeda in cats from southern Switzerland. Vet. Parasitol. 203, 287 – 293 (2014). • Guerra, D., Hegglin, D., Bacciarini, L., Schnyder, M., Deplazes, P.: Stability of the southern European border of Echinococcus multilocularis in the Alps: evidence that Microtus arvalis is a limiting factor. Parasitology doi: 10.1017/S0031182014000730 (2014). • Brunet, J., Pesson, B., Chermette, R., Regnard, P., Grimm, F., Deplazes, P., Ferreira, X., Sabou, M., Pfaff, A.W., Abou-Bacar, A., Candolfi, E.: First case of peritoneal cysticercosis in a non-human primate host (Macaca tonkeana) due to Taenia martis. Parasite Vector 7:422, 1 – 5 (2014). • Isaksson, M., Hagström, A., Armua-Fernandez, M.T., Wahlström, H., Agren, E.O., Miller, A., Holmberg, A., Lukacs, M., Casulli, A., Deplazes, P., Juremalm, M.: A semi-automated magnetic capture probe based DNA extraction and real-time PCR method applied in the Swedish surveillance of Echinococcus multilocularis in red fox (Vulpes vulpes) faecal samples. Parasite Vector 7:583, 1 – 10 (2014). • Basso, W., Handke, M., Sydler, T., Borel, N., Grimm, F., Sidler, X., Deplazes, P.: Involvement of Toxoplasma gondii in reproductive disorders in Swiss pig farms. Vet. Parasitol. 199, 272 – 277 (2015). • Hegglin, D., Bontadina, F., Deplazes, P.: Human-wildlife interactions and zoonotic transmission of Echinococcus multilocularis. Trends Parasitol. 31 (5) , 167 – 173 (2015). • Walker, R.A., Sharman, P.A., Miller, C.M. Lippuner, C., Okoniewski, M., Eichenberger, R.M., Ramakrishnan, C., Brossier, F., Deplazes, P., Hehl, A.B., Smith, N.C.: RNA Seq analysis of the Eimeria tenella gametocyte transciptome reveals clues about the molecular basis for sexual reproduction and oocyst biogenesis. BMC Genomics 16:94, doi: 10.1186/s12864-015-1298-6 (2015). • Hehl, A.B., Basso, W.U., Lippuner, C., Ramakrishnan, C., Okoniewski, M., Walker, R.A., Grigg, M.E., Smith, N.C., Deplazes, P.: Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes. BMC Genomics 16:66, doi: 10.1186/s12864-015-1225-x (2015). • Federer, K., Armua-Fernandez M.T., Hoby, S., Wenker, C., Deplazes, P.: In vivo viability of Echinococcus multilocularis eggs in a rodent model after different thermos-treatments. Exp. Parasitol. 154, 14 – 19 (2015). • Otranto, D., Cantacessi, C., Pfeffer, M., Dantas-Torres, F., Brianti, E., Deplazes, P., Genchi, C., Guberti, V., Capelli, G.: The role of wild canids and felids in spreading parasites to dogs and cats in Europe Part I: Protozoa and tick-borne agents. Vet. Parasitol. 213, 12 - 23 (2015). SRTP Proposal Naples page 38 of 105

• Otranto, D., Cantacessi, C., Dantas-Torres, F., Brianti, E., Pfeffer, M., Genchi, C., Guberti, V., Capelli, G., Deplazes, P.: The role of wild canids and felids in spreading parasites to dogs and cats in Europe. Part II: Helminths and arthropods. Vet. Parasitol. 213, 24 - 37 (2015). • Brunet, J., Benoilid, A., Kremer, S., Dalvit, C., Lefebvre, N., Hansmann, Y., Chenard, M.-P., Mathieu, B., Grimm, F., Deplazes, P., Pfaff, A.W., Abou-Bacar, A., Marescaux, C., Candolfi, E.: First case of human cerebral Taenia martis cysticercosis. J. Clin. Microbiol. 53 (8), 2756 – 2759. doi:10.1128/JCM.01033-15 (2015). • Schnyder, M., Jeffries, R., Schucan, A., Morgan, E.R., Deplazes, P.: Comparison of coprological, immunological and molecular methods for the detection of dogs infected with Angiostrongylus vasorum before and after anthelmintic treatment. Parasitology, 1 – 8,doi:10.1017/S0031182015000554 (2015). • Gori, F., Armua-Fernandez, M.T., Milanesi, P., Serafini, M., Magi, M.,Deplazes, P., Macchioni, F. : The occurrence of taeniids of wolves in Liguria (northern Italy). Int. J. Parasitol. Parasites and Wildlife 4, 252 – 255 (2015). • Liccioli, S., Giraudoux, P., Deplazes, P., Massolo, A. : Wilderness in the « city » revisited : different urbes shape transmission of Echinococcus multilocularis by altering predator and prey communities. Trends Parasitol. 31 (7), 297 – 305 (2015). • Hauser, M., Basso, W., Deplazes, P.: Kontamination landwirtschaftlicher Nutzflächen durch Hunde- und Fuchskot. Schweiz. Arch. Tierheilkd. 157 (8), 449 – 455 (2015). • Heim, C., Hertzberg, H., Butschi, A., Bleuler-Martinez, S., Aebi, M., Deplazes, P., Künzler, M., Stefanic, S.: Inhibition of Haemonchus contortus larval development by fungal lectins. Parasite Vector 8:425, 1 – 10 (2015). • Jurankova, J., Basso, W., Neumayerova, H., Frencova, A., Balaz, V., Deplazes, P., Koudela, B.: Predilection sites for Toxoplasma gondii in sheep tissues revealed by magnetic capture and real- time PCR detection. Food Microbiology 52, 150 – 153 (2015). • Gottstein, B., Stojkovic, M., Vuitton, D.A., Millon, L., Marcinkute, A., Deplazes, P.: Threat of alveolar echinococcosis to public health – a challenge for Europe. Trends Parasitol. 31(9), 407 - 412 (2015). • Eichenberger, R.M., Deplazes, P., Mathis, A.: Ticks on dogs and cats: A pet owner-based survey in a rural town in northeastern Switzerland. Ticks and Tick-borne Diseases 6, 267 – 271 (2015). • Deplazes, P., Gottstein, B., Junghanss, T.: Alveolar and cystic echinococcosis in Europe: Old burdens and new challenges. Vet. Parasitol. 213, 73 - 75 (2015). • Lurati, L., Deplazes, P., Hegglin, D., Schnyder, M.: Seroepidemiological survey and spatial analysis of the occurrence of Angiostrongylus vasorum in Swiss dogs in relation to biogeographic aspects. Vet. Parasitol. 212, 219 - 226 (2015). • Schnyder, M., Jefferies, R., Schucan, A., Morgan, E.R., Deplazes, P.: Comparison of coprological, immunological and molecular methods for the detection of dogs infected with Angiostrongylus vasorum before and after anthelmintic treatment. Parasitology 142, 1270 – 1277 (2015). • Böhm, C., Wolken, S., Schnyder, M., Basso, W., Deplazes, P., Di Cesare, A., Deuster, K., Schaper, R.: Efficacy of Emodepside/Praziquantel Spot-on (Profender®) against adult Aelurostrongylus abstrusus Nematodes in Experimentally Infected Cats. Parasitol. Res. 114, 149 – 158 (2015). • Ammann, R.W., Stumpe, K.D.M., Grimm, F., Deplazes, P., Huber, S., bertogg, K., Fischer, D.R., Müllhaupt, B.: Outcome after Discontinuing Long-Term Benzimidazole Treatment in 11 Patients SRTP Proposal Naples page 39 of 105

with Non-resectable Alveolar Echinococcosis with Negative FDG-PET/CT and Anti-EmII/3-10 Serology. : PLoS Neglected Tropical Diseases 9(9), e0003964 • Marcinkute, A., Sarkunas, M., Moks, E., Saarma, U., Jokelainen, P., Bagrade, G., Laivacuma, S., Strupas, K., Sokolovas, V., Deplazes, P. : Echinococcus infections in the Baltic region. Vet. Parasitol. 213, 121 - 131. doi: 10.1016/j.vetpar.2015.07.032 (2015). • Conraths, F.J., Deplazes, P.: Echinococcus multilocularis: Epidemiology, surveillance and state-of- the-art diagnostics from a veterinary public health perspective. Vet. Parasitol. 213, 149 – 161. doi: 10.1016/j.vetpar.2015.07.027 (2015). • Woolsey, I.D., Jensen, P.M., Deplazes, P., Kapel C.M.: Establishment and development of Echinococcus multilocularis metacestodes in the common vole (Microtus arvalis) after oral inoculation with parasite eggs. Parasitol. Int. 64(6), 571 – 575 (2015). • Woolsey, I.D., Touborg Bune, N. E., Moestrup Jensen, P., Deplazes, P., Outzen Kapel, C.M.: Echinococcus multilocularis infection in the field vole (Microtus agrestis): an ecological model for studies on transmission dynamics. Parasitol. Res. 114, 1703 – 1709 (2015). • Baneth, G., Thamsborg, S.M., Otranto, D., Guillot, J. Blaga, R., Deplazes, P., Solano-Gallego, L.: Major Parasitic Zoonoses Associated with Dogs and Cats in Europe. J. Comp. Path. 155, 1 – 21 (2016). • Woolsey, I.D., Jensen, P.M., Deplazes, P., Kapel, C.M.: Peroral Echinococcus multilocularis egg inoculation in Myodes glareolus, Mesocricetus auratus and Mus musculus (CD-1 IGS and C57BL/6j). Int. J. Parasitol. Parasites Wildl. 5(2), 158 – 163 (2016). • Armua-Fernandez, M.T., Joekel, D., Schweiger, A., Eichenberger, R.M., Matsumoto, J., Deplazes, P.: Successful intestinal Echinococcus multilocularis oncosphere invasion and subsequent hepatic metacestode establishment in resistant RccHan™:WIST rats after pharmacological immunosuppression. Parasitology 18, 1 – 9 (2016). • Alho, A.M., Pita, J., Amaro, A., Amaro, F., Schnyder, M., Grimm, F., Custódio, A.C., Cardoso, L., Deplazes, P., de Carvalho, L.M.: Seroprevalence of vector-borne pathogens and molecular detection of Borrelia afzelii in military dogs from Portugal. Parasite Vector 9(1), 225: doi: 10.1186/s13071- 016-1509-2 (2016). • Alho, A.M., Schnyder, M., Schaper, R., Meireles, J., Belo, S., Deplazes, P., de Carvalho, L.M.: Seroprevalence of circulating Angiostrongylus vasorum antigen and parasite-specific antibodies in dogs from Portugal. Parasitol. Res. 115(7), 2567 – 2572 (2016). • Wahlström, H., Comin, A., Isaksson, M., Deplazes, P.: Detection of Echinococcus multilocularis by MC-PCR: evaluation of diagnostic sensitivity and specificity without gold standard. Infect. Ecol. Epidemiol. 6:30173; doi: 10.3402/iee.v6.30173 (2016). • Eichenberger, R.M., Riond, B., Willi, B., Hofmann-Lehmann, R., Deplazes, P.: Prognostic Markers in Acute Babesia canis Infections. J. Vet. Intern. Med. 30(1), 174 – 182; doi: 10.1111/jvim.13822 (2016). • Weber, S.E., Lippuner, C., Corti, S., Deplazes, P., Hässig, M.: Klinische Epidemiologie der Kälber- Cryptosporidiose. Schweiz. Arch. Tierh. 158 (5), 341 – 350 (2016). • Federer, K., Armua-Fernandez, M.T., Gori, F., Hoby, S., Wenker, C., Deplazes. P.: Detection of Taeniid (Taenia spp., Echinococcus spp.) eggs contaminating vegetables and fruits sold in European markets and the risk for metacestode infections in captive primates. Int. J. Parasitol. Parasites and Wildlife 5, 249-253 (2016).

SRTP Proposal Naples page 40 of 105

Appendix 5: Description of Facilities, services and equipment of the Laboratory of Parasitology, Veterinary Research Institute, Hellenic Agricultural Organisation- Demeter (former NAGREF), Thessaloniki Greece; CV and publication list of Prof. Smaragda Sotiraki

Contact: Prof. Smaragda Sotiraki, DVM, PhD, DipEVPC Veterinary Research Institute, Hellenic Agricultural Organisation/DEMETER, Thessaloniki (GR)

The Hellenic Agricultural organisation/Demeter is the national body for Agricultural Research and Technology in Greece. The Veterinary Research Institute is located in Thessaloniki, Northern Greece in a Campus together with four other Research Institutes (Agricultural Research Centre, Cereal Science Institute, Soil Science & Plant Protection Institute) all of which belong to HAO framework. The Veterinary Research Institute is the principle organisation for animal health and welfare R&D and advice in Greece and also aims to reduce environmental impacts and improve food quality. The Institute has long-term experience in collaboration on the basis of interstate agreements and in the frame of EU Programs and sufficient technical and personnel capabilities to conduct research and provide training for the young researchers. The team has many years of experience in managing research projects, dealing with all sorts of human/animal health protection and to improve product quality/safety addressing EU Policy priorities. With respect to research, the VRI covers a broad spectrum of animal health and welfare, breeding for robustness traits, as well as product safety and quality assurance areas/issues, in order to create new knowledge related to the prevention and control of animal diseases and zoonoses.

The Laboratory of Veterinary Parasitology at the Veterinary Research Institute The Laboratory of Parasitology at the VRI/HAO-Demeter focuses the R&D activities on epidemiology of parasitic infections, integrated disease management (including antiparasitic treatments and alternatives solutions) and spread of parasitic zoonoses. The Laboratory is fully equipped for all standard diagnostic procedures in Veterinary Parasitology. Diagnosis of common parasites is carried out on samples brought by general practitioners, as well as colleagues from Farmers.

The approximate number of diagnostic samples (faeces, blood, skin scrapings, ectoparasites, adult parasites and other matrices like food and water) ranges from 400-500/year The animal species considered include sheep, goat, dog, cat, cattle, pig and, to a lesser extent, avian species. Several human samples as well. Procedures: -Flotation with different flotation solutions (NaCl, Zinc Sulphate, Sucrose…) -Sedimentation -McMaster and FLOTAC enumeration procedures -Modified Knott test for microfilariae detection -Baermann technique -Immunofluoresence for Giardia, Cryptosporidium spp. -IDEXX filtration water system and IMS for Giardia, Cryptosporidium spp. in water -ELISA for serology - real-time and conventional PCR -Direct examination of skin/ear scrapings -Identification protocoal for endo/ectoparasites SRTP Proposal Naples page 41 of 105

Instruments and laboratories: a fully equipped classic parasitology diagnosis lab, a dark lab for fluorescent diagnosis, a molecular parasitology lab, a tissue culture unit, a necropsy unit, several centrifuges including ultra centrifuges, etc. - 2 stereo microscopes - 2 optical microscopes - 2 fluorescent microscopes (classic and inverted) - 4 different type centrifuges - 2 Laminar flow hoods - 3 incubators and 1 CO2 incubator - ELISA reader for serology - Real-time and conventional PCR thermocyclers - HPLC (DAD, FLD - ultra low T freezers - several spectrophotometers, - image analysis systems

Parasite strains currently maintained Sheep and swine coccidia (Eimeria, Isospora), Sheep gastrointestinal nematodes (e.g. Haemonchus contortus and Trichostrongylus colubriformis)

CV and publication list of Prof. Smaragda Sotiraki Personal information Date/Place of Birth: March 21, 1967/ Thessaloniki GREECE Nationality: Greek Address: Laboratory of Parasitology, Veterinary Research Institute, Hellenic Agricultural Organisation- Demeter (former NAGREF), NAGREF Campus Thermi, PO Box 60272, 57001 Thessaloniki (Greece) Telephone(s) +30 2310 365 373 Fax(es) +30 2310 365 371 E-mail(s) [email protected], [email protected]

Education: 2003 De Facto Diplomat of the European Veterinary Parasitology College, DF0064 1999 Doctoral Thesis, Faculty of Veterinary Medicine, Aristotle University, degree: excellent. Thesis subject: «Study on the epidemiology of Dicrocoelium dendriticum in sheep and goats in certain regions of central Macedonia, Greece» 1991 Degree of Veterinary Medicine, Aristotle University

Employment 2000 → today Senior Research Scientist, Head Laboratory of Parasitology, Veterinary Research Institute, Hellenic Agricultural Organisation- Demeter (former NAGREF), Thessaloniki Greece 1999 – 2007 Teaching Assistant Professor, Veterinary Faculty, University of Thessaly, Greece 2002 - 2004 Assistant Professor, Danish Centre for Experimental Parasitology SRTP Proposal Naples page 42 of 105

1991 - 2000 Laboratory of Parasitology and Parasitic Diseases Veterinary Medicine School, Aristotle University, Thessaloniki, Greece (Research Fellow and Teaching Assistant)

Post-graduate studies/seminars

2002-2004 Post doctoral studies on Piglet coccidiosis due to Isospora suis (Marie Curie Post Doctoral fellowship) Danish Centre for Experimental Parasitology, RVAU Denmark 1999 Training on Entomology and usage of Scanning Electron Microscope (SEM) at the Natural History Museum, London U.K.. (Scholarship from the E.C. Large Scale Facilities Program managed by the Natural History Museum 1998 Experimental helminthology: an introduction to procedures and analyses” Danish Centre for Experimental Parasitology, Copenhagen, Denmark 1998 Courses on Molecular Biology, Faculty of Biological Sciences, Aristotle University, Thessaloniki, Greece 1997 Diagnostic Parasitology Course” at London School of Hygiene and Tropical Medicine, London, U.K. 1995 International Training Course on Echinococcus, Cyprus 1994 Seventh International Training Course on the Identification of Helminth Parasites of Economic Importance» which was held at the International Institute of Parasitology in U.K. 1995 Protection of laboratory animals-usage in clinical research and diagnostics- alternatives Part II, Thessaloniki 1993 Protection of laboratory animals-usage in clinical research and diagnostics- alternatives, Part I, Thessaloniki

Research experience in Veterinary Parasitology Dr Sotiraki is leading the VRI Team and has over 15 years R&D and she has significant managerial experience in research projects. Overall, she has participated in more than 20 (coordinating most of them) major R&D programmes at National and International level.

Selected Research Projects: 1. Research of pathogenicity and epizootiology (at the final and intermediate host) of Dicrocoelium dendriticum in small ruminants» (981/5-2-91) – funded by the Greek General Secretariat for Science and Technology 2. “Environmentally sensitive approaches to nematode parasite control in sustainable agricultural systems” - E.U research project (PL96-1485) 3. «A comparative study on the efficacy of various dicrocelicides drugs and the application of the most effective one in vivo under sustainable Greek agricultural systems against Dicrocoelium dendriticum» -funded by the Greek General Secretariat for Science and Technology. 4. «Epizootiological survey and control of sheep and goats parasites in Municipality of Kavala» - funded by EU through the Municipality of Kavala, Greece 5. «Cystic echinococcosis – hydatidosis in the Eastern Mediteranean and Midle East» - EU research project ERB IC18-CT98-0354. 6. A study of the epidemic of livestock myiasis on the island of Crete: identification of the parasitic flies, epidemiology of infestations and strategies of prevention and control – funded by NAGREF and British Council (project leader) SRTP Proposal Naples page 43 of 105

7. “A study of the epidemic of livestock myiasis on the island of Crete: identification of the parasitic flies, epidemiology of infestations and strategies of prevention and control” – Greek-Hungary bilateral research collaboration (project leader) 8. «Biological control of nematode parasitism in dairy ewes using nematophagous fungi» - funded by NAGREF and INRA (project leader) 9. «Field trial to assess the efficacy of CLIK® for the prevention of fly strikes due to Wohlfahrtia magnifica on sheep in Greece» - funded by Novartis Animal Health Inc, Switzerland (project leader) 10. «Field trial to assess the efficacy of CLIK® for the prevention of wohlfahrtiosis on sheep – Part II (application management)» - funded by Novartis Animal Health Inc, Switzerland (project leader) 11. Pig coccidiosis: Regulating transmission, improving animal health and product quality – funded by Danish Bacon and Meat Council (project leader) 12. European Reintegration Grant (ERG) Marie Curie project entitled: Piglet Isosporosis: Integrated disease management to improve animal welfare, food quality and safety (project leader) 13. Marie Curie Training Network. Acronym: Healthy Hay, Full title: The reinvention of sainfoin: an example of novel resource for sustainable agriculture» (project leader for Greece) 14. Development of integrated livestock breeding and management strategies to improve animal health, product quality and performance in European organic and ‘low input’ milk, meat and egg production (FP7 Collaborative project) (project leader for Greece) and COST Actions: COST Action 833 “Mange and Myiasis in Livestock” as expert scientist and COST Action 854 “Protozoal reproduction losses in farm ruminants” as a member of the management committee COST Action FA0805 Goat-parasite interactions: from knowledge to control (CAPARA) (Chair of the Management Committee). COST Action TD1303 European Network for Neglected Vectors and Vector Borne Infection (Vice Chair of the Management Committee).

Teaching experience in Veterinary Parasitology From 1994 to 2007, she has been teaching Vet Parasitology at the Vet Schools of Thessaloniki and Karditsa and since 2004 to present she co-supervised 10 PhD students (4 completed) in collaboration with various Universities like AUTH, University of Thessaly, Agricultural University of Athens, Ghent University, Vet School Toulouse, Vet School Vienna, several Master Thesis and graduate thesis. Dr. Sotiraki has been also teaching Veterinary Parasitology in various educational courses for Veterinarians or other professionals. Dr. Sotiraki is a diplomate of the European Veterinary Parasitology College and for over 8 years Member of the Educational Committee of the College. Memberships: • Hellenic Veterinary Medical Society • World Association for the Advancement of Veterinary Parasitology (WAAVP) • International Committee for Trichinellosis

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Publication List (2012-2016) • Tzanidakis, N., Maksimov, P., Conraths, F.J., Kiossis, E., Brozos, C., Sotiraki, S., Schares, G. Toxoplasma gondii in sheep and goats: Seroprevalence and potential risk factors under dairy husbandry practices (2012) Veterinary Parasitology, 190 (3-4), pp. 340-348.Sotiraki, S., Hall, M.J.R. A review of comparative aspects of myiasis in goats and sheep in Europe (2012) Small Ruminant Research, 103 (1), pp. 75-83. • Tzanidakis N, Sotiraki S, Claerebout E, Ehsan A, Voutzourakis N, Kostopoulou D, Stijn C, Vercruysse J, Geurden T.Occurrence and molecular characterization of Giardia duodenalis and Cryptosporidium spp. in sheep and goats reared under dairy husbandry systems in Greece. Parasite. 2014;21:45. doi: 10.1051/parasite/2014048. Epub 2014 Sep. • Ready, P.D., Wardhana, A.H., Adams, Z.J.O., Sotiraki, S., Hall, M.J.R.Improved method for screening mitochondrial cytochrome b markers to identify regional populations of the Old World screwworm fly and other myiasis agents (2014) Acta Tropica, Article in Press. • Papa, A., Chaligiannis, I., Kontana, N., Sourba, T., Tsioka, K., Tsatsaris, A., Sotiraki, S. A novel AP92-like Crimean-Congo hemorrhagic fever virus strain, Greece (2014) Ticks and Tick-borne Diseases, 5 (5), pp. 590-593. • Devleesschauwer B, Allepuz A, Dermauw V, Johansen MV, Laranjo-González M, Smit GS, Sotiraki S, Trevisan C, Wardrop NA, Dorny P, Gabriël S. Taenia solium in Europe: Still endemic? Acta Trop. 2015 Aug 12. pii: S0001-706X(15)30083-8. doi: 10.1016/j.actatropica.2015.08.006. • Hoste H, Torres-Acosta JF, Sandoval-Castro CA, Mueller-Harvey I, Sotiraki S, Louvandini H, Thamsborg SM, Terrill TH.Tannin containing legumes as a model for nutraceuticals against digestive parasites in livestock.Vet Parasitol. 2015 Aug 15, 212, (1-2), 5-17. • Chaligiannis I, Maillard S, Boubaker G, Spiliotis M, Saratsis A, Gottstein B, Sotiraki S. Echinococcus granulosus infection dynamics in livestock of Greece. Acta Trop. 2015 Oct;150:64- 70. doi: 10.1016/j.actatropica.2015.06.021. • Kostopoulou D, Casaert S, Tzanidakis N, van Doorn D, Demeler J, von Samson-Himmelstjerna G, Saratsis A, Voutzourakis N, Ehsan A, Doornaert T, Looijen M, De Wilde N, Sotiraki S, Claerebout E, Geurden T. The occurrence and genetic characterization of Cryptosporidium and Giardia species in foals in Belgium, The Netherlands, Germany and Greece. Vet Parasitol. 2015 Jul 30; 211 (3-4), 170-174. • Papa A, Kontana A, Tsioka K, Chaligiannis I, Sotiraki S. Novel phleboviruses detected in ticks, Greece. Ticks Tick Borne Dis. 2016 Feb 24. pii: S1877-959X(16)30031-0. doi: 10.1016/j.ttbdis.2016.02.017. • Saratsis A, Voutzourakis N, Theodosiou T, Stefanakis A, Sotiraki S. The effect of sainfoin (Onobrychis viciifolia) and carob pods (Ceratonia siliqua) feeding regimes on the control of lamb coccidiosis. Parasitol Res. 2016 Feb 26. • Srednicka-Tober, D., Barański, M., Seal, C., Sanderson, R., Benbrook, C., Steinshamn, H., Gromadzka-Ostrowska, J., Rembiałkowska, E., Skwarło-Sońta, K., Eyre, M., Cozzi, G., Krogh Larsen, M., Jordon, T., Niggli, U., Sakowski, T., Calder, P.C., Burdge, G.C., Sotiraki, S., Stefanakis, A., Yolcu, H., Stergiadis, S., Chatzidimitriou, E., Butler, G., Stewart, G., Leifert, C. Composition differences between organic and conventional meat: A systematic literature review and meta-analysis (2016) British Journal of Nutrition, 115 (6), pp. 994-1011. • Srednicka-Tober, D., Barański, M., Seal, C.J., Sanderson, R., Benbrook, C., Steinshamn, H., Gromadzka-Ostrowska, J., Rembiałkowska, E., Skwarło-Sońta, K., Eyre, M., Cozzi, G., Larsen, SRTP Proposal Naples page 45 of 105

M.K., Jordon, T., Niggli, U., Sakowski, T., Calder, P.C., Burdge, G.C., Sotiraki, S., Stefanakis, A., Stergiadis, S., Yolcu, H., Chatzidimitriou, E., Butler, G., Stewart, G., Leifert, C. Higher PUFA and n-3 PUFA, conjugated linoleic acid, α-tocopherol and iron, but lower iodine and selenium concentrations in organic milk: A systematic literature review and meta- and redundancy analyses (2016) British Journal of Nutrition, 115 (6), pp. 1043-1060.

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Appendix 6: CV and Publication list of the Program Director, Prof. Giuseppe Cringoli

Date/Place of Birth: October 10th, 1952/ Bisaccia (AV) Nationality: Italian

Education: 1978 Degree in Veterinary Medicine from the University of Naples Federico II, Naples, Italy

Employment 1981-1991: Research in Pathology and Profilaxys of Infectious Diseases of Companion Animals, University of Naples, Italy 1991-1993: Associate Professor of Veterinary Parasitology and Parasitic Diseases, University of Molise, Campobasso, Italy 1993-2001: Associate Professor of Veterinary Parasitology and Parasitic Diseases, University of Naples, Naples, Italy 2001-present: Full Professor of Veterinary Parasitology and Parasitic Diseases, University of Naples, Naples, Italy 2001-present: Director of the Regional Center for Monitoring of Parasitic Diseases (Campania Region) 2013-present: Director of the Interuniversity Center of Researchers in Parasitology

Research experience in Veterinary Parasitology Prof.Cringoli’s main research interests are epidemiology, diagnosis and control of protozoa, helminthes and artropoda of veterinary interest. He is inventor of the FLOTAC, Mini-FLOTAC and Fill-FLOTAC, new diagnostic methods for uro/copromicroscopic diagnosis of parasites in animals and humans. Furthermore he introduced the application of Geographical Information Systems (GIS) to the epidemiology of parasitic diseases. The scientific production in veterinary parasitology consists of more than 650 publications: papers published on national and international scientific journals and proceedings of national and international conferences.

Teaching experience in Veterinary Parasitology From 2001 to the present, Prof. Giuseppe Cringoli has been responsible for the teaching of Veterinary Parasitology and Parasitic Diseases at the University of Naples Federico II and was tutor for students for their final year thesis and PhD thesis. Since 2012 he is Coordinator of the PhD course of “Veterinary Sciences”. Prof. Giuseppe Cringoli is a diplomate of the European Veterinary Parasitology College.

GRANTS HELD: He was scientific coordinator and responsible of Research Unit of research projects of national and international importance in veterinary and human field.

Memberships: • Società Italiana di Parassitologia (SOIPA) • World Association for the Advancement of Veterinary Parasitology (WAAVP) SRTP Proposal Naples page 47 of 105

• Società Italiana delle Scienze Veterinarie (SISVet); • Società Italiana di Patologia e di Allevamento degli Ovini e dei Caprini (SIPAOC) • Member of the Italian Team of the European Scientific Counsel Companion Animal Parasites (ESSCAP) • International Society of Geospatial Health (GnosisGIS)

Contribution to veterinary medical associations and journals

Giuseppe Cringoli is honorary member of the International Scientific Society GnosisGIS and co- founder (as well as member of the editorial board) of the international scientific journal Geospatial Health. He is series Editor of “Mappe Parassitologiche”, Associate Editor BMC Veterinary Researchand member of the Editorial Board of several International Scientific Journals

Publication List (2012-2016):

• Duthaler U, Huwyler J, Rinaldi L, Cringoli G, Keiser J. Evaluation of the pharmacokinetic profile of artesunate, artemether and their metabolites in sheep naturally infected with Fasciola hepatica. Vet Parasitol. 2012; 186 (3-4): 270-80. • Rinaldi L, Mihalca AD, Cirillo R, Maurelli MP, Montesano M, Capasso M, Cringoli G. FLOTAC can detect parasitic and pseudoparasit elements in reptiles. Exp Parasitol. 2012; 130(3): 282-4. • Steinmann P, Cringoli G, Bruschi F, Matthys B, Lohourignon LK, Castagna B, Maurelli MP, Morgoglione ME, Utzinger J, Rinaldi L. FLOTAC for the diagnosis of Hymenolepis spp. infection: proof-of-concept and comparing diagnostic accuracy with other methods. Parasitol Res. 2012; 111(2):749-54. • Dipineto L, Capasso M, Maurelli MP, Russo TP, Pepe P, Capone G, Fioretti A, Cringoli G, Rinaldi L. Survey of co-infection by Salmonella and oxyurids in tortoises. BMC Vet Res. 2012; 8:69. • Levecke B, Rinaldi L, Charlier J, Maurelli MP, Bosco A, Vercruysse J, Cringoli G.The bias, accuracy and precision of faecal egg count reduction test results in cattle using McMaster, Cornell-Wisconsin and FLOTAC egg counting methods.Vet Parasitol. 2012;188(1-2):194-9. • Rinaldi L, Gonzales S, Guerrero J, Aquilera LC, Musella V, Genchi C, Cringoli G. A One- Health integrated approach to control fascioliasis in the Cajamarca valley of Perù. Geospat Health. 2012, 6(3): S67-73. • Trapani F, Paciello O, Papparella S, Rinaldi L, Cringoli G, Maiolino P. Histopathological, histochemical and immunohistochemical findings of the small intestine in goats naturally infected by Trichostrongylus colubriformis.Vet Parasitol. 2013, 191(3-4):390-3. • Catelan D, Rinaldi L, Musella V, Cringoli G, Biggeri A. Statistical approaches for farm and parasitic risk profiling in geographical veterinary epidemiology.Stat Methods Med Res. 2012, 21(5): 531-43. • Rinaldi L, Capasso M, Mihalca AD, Cirillo R, Cringoli G, Cacciò S. Prevalence and molecular identification of Cryptosporidium isolates from pet lizards and snakes in Italy.Parasite. 2012,19(4):437-40. • Rinaldi L, Maurelli MP, Musella V, Bosco A, Cortes H, Cringoli G. First cross-sectional serological survey on Besnoitia besnoiti in cattle in Italy.Parasitol Res. 2013,112(4):1805-7. SRTP Proposal Naples page 48 of 105

• Silva LMR, Vila-Viscosa MJM, Maurelli MP, Morgoglione ME, Cortes HCE, Cringoli G, Rinaldi L. Mini-FLOTAC for the diagnosis of Eimeria infection in goats: An alternative to McMaster. Small Ruminant Research 2013, 114: 280-283. • Demeler J, Ramünke S, Wolken S, Ianiello D, Rinaldi L, Gahutu JB, Cringoli G, von Samson-Himmelstjerna G, Krücken J. Discrimination of gastrointestinal nematode eggs from crude fecal egg preparations by inhibitor-resistant conventional and real-time PCR.PLoS One2013,8(4):e61285. • Dipineto L,Rinaldi L,Bosco A,Russo TP,Fioretti A,Cringoli G. Co-infection by Escherichia coli O157 and gastrointestinal strongyles in sheep. Vet J.2013, 197(3):884-5. • Cringoli G, Rinaldi L, Albonico M, Bergquist R, Utzinger J. Geospatial (s)tools: integration of advanced epidemiological sampling and novel diagnostics.Geospat Health. 2013, 7(2):399-404. • Albonico M, Rinaldi L, Sciascia S, Morgoglione ME, Piemonte M, Maurelli MP, Musella V, Utzinger J, Ali SM, Ame SM, Cringoli G. Comparison of three copromicroscopic methods to assess albendazole efficacy against soil-transmitted helminth infections in school-aged children on Pemba island. Trans R Soc Trop Med Hyg. 2013, 107(8):493-501. • Barda B, Zepherine H, Rinaldi L, Cringoli G, Burioni R, Clementi M, Albonico M. Mini- FLOTAC and Kato-Katz: helminth eggs watching on the shore of lake Victoria. Parasit Vectors. 2013,6(1):220. • Barda BD, Rinaldi L, Ianniello D, Zepherine H, Salvo F, Sadutshang T, Cringoli G, Clementi M, Albonico M. Mini-FLOTAC, an Innovative Direct Diagnostic Technique for Intestinal Parasitic Infections: Experience from the Field.PLoS Negl Trop Dis. 2013, 7(8):e2344. • Morgan ER, Charlier J, Hendrickx G, Biggeri A, Catalan D, von Samson-Himmelstjerna G, Demeler J, Müller E, van Dijk J, Kenyon F, Skuce P, Höglund J, O’Kiely P, van Ranst B, de Waal T, Rinaldi L, Cringoli G, Hertzberg H, Torgerson P, Wolstenholme A, Vercruysse J. Global change and helminth Infections in grazing ruminants in Europe: impacts, trends and sustainable solutions. Agriculture 2013, 3, 484-502. • Beato S, Parreira R, Roque C, Gonçalves M, Silva L, Maurelli MP, Cringoli G, Grácio MA. Echinococcus granulosus in Portugal: The first report of the G7 genotype in cattle.Vet Parasitol. 2013, 198(1-2):235-9. • Meister I, Duthaler U, Huwyler J, Rinaldi L, Bosco A, Cringoli G, Keiser J.Efficacy and pharmacokinetics of OZ78 and MT04 against a natural infection with Fasciola hepatica in sheep.Vet Parasitol 2013,198(1-2):102-10. • Barda B, Ianniello D, Salvo F, Sadutshang T, Rinaldi L, Cringoli G, Burioni R, Albonico M."Freezing" parasites in pre-Himalayan region, Himachal Pradesh: Experience with mini- FLOTAC. Acta Trop. 2013; 130C:11-16. • Cuervo PF, Sierra RM, Waisman V, Gerbeno L, Sidoti L, Albonico F, Mariconti M, Mortarino M, Pepe P, Cringoli G, Genchi C, Rinaldi L. Detection of Dirofilaria immitis in mid-western arid Argentina. Acta Parasitol 2013; 58(4): 612-14. • Cuervo PF, Fantozzi MC, Di Cataldo S, Cringoli G, Mera y Sierra R, Rinaldi L. Analysis of climate and extrinsic incubation of Dirofilaria immitis in southern South America. Geospatial Health 2013;8: 175-81. SRTP Proposal Naples page 49 of 105

• Bazzocchi C, Mariconti M, Sassera D, Rinaldi L, Martin E, Cringoli G, Urbanelli S, Genchi C, Bandi C, Epis S. Molecular and serological evidence for the circulation of the tick symbiont Midichloria (Rickettsiales: Midichloriaceae) in different mammalian species. Parasit Vectors.2013; 6(1): 350. • d'Ovidio D,Rinaldi L,Ianniello D,Donnelly TM,Pepe P,Capasso M,Cringoli G. FLOTAC for diagnosis of endo-parasites in pet squirrels in southern Italy. Vet Parasitol.2014, 200(1- 2):221-4. • Dipineto L, Borrelli L, Pepe P, Fioretti A, Caputo V, Cringoli G, Rinaldi L. Synanthropic birds and parasites. Avian Dis. 2013, 57(4):756-8. • Rinaldi L, Morgan ER, Bosco A, Coles GC, Cringoli G. The maintenance of anthelmintic efficacy in sheep in a Mediterranean climate.Vet Parasitol. 2014, 203(1-2):139-43. • Rinaldi L, CringoliG. Exploring the interface between diagnostics and maps of neglected parasitic diseases.Parasitology 2014, 28:1-8. • Bosco A, Rinaldi L, Musella V, Pintus D, Santaniello M, Morgoglione ME, Zacometti G, Cringoli G. Helminths in Sheep on Farms of the Basilicata Region of Southern Italy. Trends in Veterinary Sciences 2013, p 91-94. • d'Ovidio D, Pepe P, Ianniello D, Noviello E, Quinton JF, Cringoli G, Rinaldi L.First survey of endoparasites in pet ferrets in Italy.Vet Parasitol. 2014, 203(1-2):227-30. • Maesano G, Capasso M, Ianniello D, Cringoli G, Rinaldi L.Parasitic infections detected by FLOTAC in zoo mammals from Warsaw, Poland.Acta Parasitol.59(2):343-53. • Barda B, Ianniello D, Zepheryne H, Rinaldi L, CringoliG, Burioni R, Albonico M. Parasitic infections on the shore of Lake Victoria (East Africa) detected by Mini-FLOTAC and standard techniques.Acta Trop. 2014;137:140-6. • Djokic V, Klun I, Musella V, Rinaldi L, Cringoli G, Sotiraki S, Djurkovic-Djakovic O. Spatial epidemiology of Toxoplasma gondii infection in goats in Serbia.Geospat Health. 2014, 8(2):479-88. • Barda B, Cajal P, Villagran E, Cimino R, Juarez M, Krolewiecki A, Rinaldi L, CringoliG, Burioni R, Albonico M.Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north Argentina.Parasit Vectors. 2014; 7(1):271. • Rinaldi L, Levecke B, Bosco A, Ianniello D, Pepe P, Charlier J, Cringoli G, Vercruysse J.Comparison of individual and pooled faecal samples in sheep for the assessment of gastrointestinal strongyle infection intensity and anthelmintic drug efficacy using McMaster and Mini-FLOTAC. Vet Parasitol. 2014; 205(1-2):216-23. • Maurelli MP, Rinaldi L, Alfano S, Pepe P, Coles GC, Cringoli G. Mini-FLOTAC, a new tool for copromicroscopic diagnosis of common intestinal nematodes in dogs. Parasit Vectors. 2014; 7:356. • Musella V, Rinaldi L, Lagazio C, Cringoli G, Biggeri A, Catelan D. On the use of posterior predictive probabilities and prediction uncertainty to tailor informative sampling for parasitological surveillance in livestock.Vet Parasitol. 2014; 205(1-2):158-68. • Steinmann P, Rinaldi L, Cringoli G, Du ZW, Marti H, Jiang JY, Zhou H, Zhou XN, Utzinger J.Morphological diversity of Trichuris spp. eggs observed during an anthelminthic drug trial in Yunnan, China, and relative performance of parasitologic diagnostic tools. Acta Trop. 2014;pii: S0001-706X(14)00279-4. SRTP Proposal Naples page 50 of 105

• Maurelli MP,RinaldiL, Rubino G, Lia R, Musella V,CringoliG.FLOTAC and Mini- FLOTAC for uro-microscopic diagnosis of Capillaria plica (syn. Pearsonema plica) in dogs.BMC Res Notes. 2014; 7(1):591. • Bosco A, Rinaldi L, Maurelli MP, Musella V, Coles GC, Cringoli G. The comparison of FLOTAC, Fecpak and McMaster techniques for nematode egg counts in cattle. Acta Parasitol. 2014;59(4):625-8. • Rinaldi L, Cortese L, Meomartino L, Pagano TB, Pepe P, Cringoli G, Papparella S. Angiostrongylus vasorum: epidemiological, clinical and histopathological insights. BMC Vet Res. 2014;10(1):236. • Djokic V, Blaga R, Rinaldi L, Le Roux D, Ducry T, Maurelli MP, Perret C, Djurkovic Djakovic O,Cringoli G, Boireau P.Mini-FLOTAC for counting Toxoplasma gondii oocysts from cat feces - Comparison with cell counting plates.Exp Parasitol. 2014;147C:67-71. • Mitrea IL, Ionita M, Costin II, Predoi G, Avram E, Rinaldi L, Maurelli MP, Cringoli G, Genchi C.Occurrence and genetic characterization of Echinococcus granulosus in naturally infected adult sheep and cattle in Romania.Vet Parasitol. 2014;206(3-4):159-166. • Neves JH, Carvalho N, Rinaldi L, Cringoli G, Amarante AF. Diagnosis of anthelmintic resistance in cattle in Brazil: A comparison of different methodologies.Vet Parasitol. 2014;206(3-4):216-226. • Godber OF, Phythian CJ, Bosco A, Ianniello D, Coles G, Rinaldi L, Cringoli G. A comparison of the FECPAK and Mini-FLOTAC faecal egg counting techniques.Vet Parasitol. 2014; 207(3-4):342-5. • Rinaldi L, Pennacchio S, Musella V, Maurelli M, La Torre F, Cringoli G. Helminth control in kennels: is the combination of milbemycin oxime and praziquantel a right choice? Parasit Vectors. 2015; 8(1):30. • d'Ovidio D, Noviello E, Ianniello D, Cringoli G, Rinaldi L. Survey of endoparasites in pet guinea pigs in Italy.Parasitol Res. 2015; 114(3):1213-6. • Cuervo PF, Rinaldi L, Cringoli G. Modeling the extrinsic incubation of Dirofilaria immitis in South America based on monthly and continuous climatic data. Vet Parasitol. 2015; 209(1-2):70-5. • Rinaldi L, Biggeri A, Musella V, De Waal T, Hertzberg H, Mavrot F, Torgerson PR, Selemetas N, Coll T, Bosco A, Grisotto L, Cringoli G, Catelan D. Sheep and Fasciola hepatica in Europe: the GLOWORM experience. Geospat Health. 2015; 9(2):309-317. • Bosco A, Rinaldi L, Musella V, Amadesi A, Cringoli G. Outbreak of acute fasciolosis in sheep farms in a Mediterranean area arising as a possible consequence of climate change.Geospat Health. 2015; 9(2):319-324. • Rinaldi L, Hendrickx G, Cringoli G, Biggeri A, Ducheyne E, Catelan D, Morgan E, Williams D, Charlier J, Von Samson-Himmelstjerna G, Vercruysse J.Mapping and modelling helminth infections in ruminants in Europe: experience from GLOWORM.Geospat Health. 2015;9(2):257-259. • Rinaldi L, Catelan D, Musella V, Cecconi L, Hertzberg H, Torgerson PR, Mavrot F, De Waal T, Selemetas N, Coll T, Bosco A, Biggeri A, Cringoli G. Haemonchus contortus: spatial risk distribution for infection in sheep in Europe.Geospat Health. 2015;9(2):325-331. SRTP Proposal Naples page 51 of 105

• Bolajoko MB, Rose H, Musella V, Bosco A, Rinaldi L, Van Dijk J, Cringoli G, Morgan ER.The basic reproduction quotient (Q0) as a potential spatial predictor of the seasonality of ovine haemonchosis.Geospat Health. 2015;9(2):333-350. • Barda B, Albonico M, Ianniello D, Ame SM, Keiser J, Speich B, Rinaldi L, Cringoli G, Burioni R, Montresor A, Utzinger J. How Long Can Stool Samples Be Fixed for an Accurate Diagnosis of Soil-Transmitted Helminth Infection Using Mini-FLOTAC?PLoS Negl Trop Dis. 2015; 9(4):e0003698. • Lima V.F., Cringoli G., Rinaldi L., Monteiro M.F., Calado A.M., Ramos R.A., Meira- Santos P.O., Alves L.C. A comparison of mini-FLOTAC and FLOTAC with classic methods to diagnosing intestinal parasites of dogs from Brazil. Parasitol Res. 2015; 114(9):3529-33. • Bosco A., Rinaldi L., Cappelli G., Saratsis A., Nisoli L., Cringoli G. Metaphylactic treatment strategies with toltrazuril and diclazuril and growth performance of buffalo calves exposed to a natural eimeria infection.Vet Parasitol. 2015;212(3-4):408-10. • Pepe P., Castellano M., Alfano S., Della Pepa M.E., Tirino V., Piemonte M., Desiderio V., Zappavigna S., Galdiero M., Caraglia M., Cringoli G., Rinaldi L., Galdiero M. Dicrocoelium dendriticum induces autophagic vacuoles accumulation in human hepatocarcinoma cells.Vet Parasitol. 2015; 212(3-4):175-80. • d'Ovidio D., Noviello E., Pepe P., Del Prete L., Cringoli G., Rinaldi L. Survey of Hymenolepis spp. in pet rodents in Italy.Parasitol Res. 2015; 114(12):4381-4. • Borrelli L., Dipineto L., Rinaldi L., Romano V., Noviello E., Menna L.F., Cringoli G., Fioretti A. New diagnostic insights for Macrorhabdus ornithogaster infection.J Clin Microbiol.2015; 53(11):3448-50. • Ramos RA, Lima VF, Monteiro MF, de Andrade Santana M, Lepold R, Faustino MA, Rinaldi L, Cringoli G, Alves LC. New insights into diagnosis of Platynosomum fastosum (Trematoda: Dicrocoeliidae) in cats.Parasitol Res. 2016; 115(2):479-82. • Del Prete L, Maurelli MP, Pennacchio S, Bosco A, Musella V, Ciuca L, Cringoli G, Rinaldi L. Dirofilaria immitis and Angiostrongylus vasorum: the contemporaneous detection in kennels. BMC Vet Res. 2015; 11(1):305. • Gualdieri L, Piemonte M, Alfano S, Maffei R, Della Pepa ME, Rinaldi L, Galdiero M, Galdiero M, Cringoli G. Immigrants living in an urban milieu with sanitation in Southern Italy: persistence and transmission of intestinal parasites. Parasitol Res. 2016; 115(3):1315- 23. • Lima VF, Ramos RA, Lepold R, Cringoli G, Rinaldi L, Faustino MA, Alves LC. Use of the FLOTAC technique to diagnosing parasites of the urinary tract of dogs. Parasitol Res. 2016; 115(4):1737-9. • Cecconi L, Biggeri A, Grisotto L, Berrocal V, Rinaldi L, Musella V, Cringoli G, Catelan D. Preferential sampling in veterinary parasitological surveillance. Geospat Health. 2016; 11(1):412. • Condoleo R, Musella V, Maurelli MP, Bosco A, Cringoli G, Rinaldi L. Mapping, cluster detection and evaluation of risk factors of ovine toxoplasmosis in Southern Italy. Geospat Health. 2016, 11(2):432. • Kenyon F, Rinaldi L, McBean D, Pepe P, Bosco A, Melville L, Devin L, Mitchell G, Ianniello D, Charlier J, Vercruysse J, Cringoli G, Levecke B. Pooling sheep faecal samples SRTP Proposal Naples page 52 of 105

for the assessment of anthelmintic drug efficacy using McMaster and Mini-FLOTAC in gastrointestinal strongyle and Nematodirus infection. Vet Parasitol. 2016, 225:53-60. • Ciucă L, Genchi M, Kramer L, Mangia C, Miron LD, Prete LD, Maurelli MP, Cringoli G, Rinaldi L. Heat treatment of serum samples from stray dogs naturally exposed to Dirofilaria immitis and Dirofilaria repens in Romania. Vet Parasitol. 2016, 225:81-5. • Coulibaly JT, Ouattara M, Becker SL, Lo NC, Keiser J, N'Goran EK, Ianniello D, Rinaldi L, Cringoli G, Utzinger J. Comparison of sensitivity and faecal egg counts of Mini-FLOTAC using fixed stool samples and Kato-Katz technique for the diagnosis of Schistosoma mansoni and soil-transmitted helminths. Acta Trop. 2016 Aug 30. pii: S0001- 706X(16)30661-1. • Chaligiannis I, Musella V, Rinaldi L, Cringoli G, de la Fuente J, Papa A, Sotiraki S. Species diversity and spatial distribution of ixodid ticks on small ruminants in Greece. Parasitol Res. 2016 Sep 22. [Epub ahead of print]. • Ramünke S, Melville L, Rinaldi L, Hertzberg H, de Waal T, von Samson-Himmelstjerna G, Cringoli G, Mavrot F, Skuce P, Krücken J, Demeler J. Benzimidazole resistance survey for Haemonchus, Teladorsagia and Trichostrongylus in three European countries using pyrosequencing including the development of new assays for Trichostrongylus. Int J Parasitol Drugs Drug Resist. 2016, 6(3):230-240. • Ciucă L, Musella V, Miron LD, Maurelli MP, Cringoli G, Bosco A, Rinaldi L. Geographic distribution of canine heartworm (Dirofilaria immitis) infection in stray dogs of eastern Romania. Geospat Health. 2016, 11(3):499. • d'Ovidio D, Pantchev N, Noviello E, Del Prete L, Maurelli MP, Cringoli G, Rinaldi L. Survey of Baylisascaris spp. in captive striped skunks (Mephitis mephitis) in some European areas. Parasitol Res. 2017, 116(2):483-486. • Deplazes P, Rinaldi L, Alvarez Rojas CA, Torgerson PR, Harandi MF, Romig T, Antolova D, Schurer JM, Lahmar S, Cringoli G, Magambo J, Thompson RC, Jenkins EJ. Global Distribution of Alveolar and Cystic Echinococcosis. Adv Parasitol. 2017, 95:315-493.

SRTP Proposal Naples page 53 of 105

Appendix 7: CV and Publication list of Supervisor of the SRTP Prof. Laura Rinaldi

Date/Place of Birth: November 4th, 1973/ Naples Nationality: Italian

Education: 1997 Degree in Biological Sciences from the University of Naples Federico II, Naples, Italy 2001 Specialization in Applied Biotechnologies at the University of Naples Federico II 2014 PhD in Veterinary Sciences at the University of Ghent, Belgium

Employment 1998-2003 Research Fellowship in Parasitology and Parasitic Diseases, University of Naples Federico II, Naples, Italy 2004: Researcher of Veterinary Parasitology and Parasitic Diseases, University of Naples Federico II, Naples, Italy 2015: Associate Professor of Veterinary Parasitology and Parasitic Diseases, University of Naples Federico II, Naples, Italy

Research experience in Veterinary Parasitology Prof.Rinaldi’s main research interests are epidemiology, diagnosis and control of protozoa, helminthes and artropoda of veterinary interest. She has worked on the development and implementation of new tools for the parasitological diagnosis. In particular she participated to the development and validation of FLOTAC and Mini-FLOTAC techniques for qualitative and quantitative copromicroscopic diagnosis of helminths and intestinal protozoa in animals and humans. The scientific production in veterinary parasitology consists of more than 350 publications: papers published on national and international scientific journals and proceedings of national and international conferences. In 2011 she received the International “Peter Nansen Young Scientist” Award given by the World Association for the Advancement of Veterinary Parasitology (WAAVP).

Teaching experience in Veterinary Parasitology From 2004 to the present, Prof. Laura Rinaldi has been responsible for the teaching of Veterinary Parasitology and Parasitic Diseases at the University of Naples Federico II and was tutor for students for their final year thesis. Since 2016 she is ERASMUS coordinator at Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy Prof. Laura Rinaldi is an Associate Member of the European Veterinary Parasitology College

GRANTS HELD: She was scientific coordinator and responsible of Research Unit of research projects of national and international importance in veterinary and human field.

Memberships: • Società Italiana di Parassitologia (SOIPA) • World Association for the Advancement of Veterinary Parasitology (WAAVP) • International Society for Geospatial Health (GnosisGIS) SRTP Proposal Naples page 54 of 105

• Società Italiana delle Scienze Veterinarie (SISVet)

Contribution to veterinary medical associations and journals Laura Rinaldi is the President of the International Society for Geospatial Health and Vice-President of the LiHRA. She is one of the co-founders and Associate Editor of the Journal Geospatial Health and Section Editor of the Journal BMC Veterinary Research, Associate Member of the European Veterinary Parasitology College.

Publication List (2012-2016) • Duthaler U, Huwyler J, Rinaldi L, Cringoli G, Keiser J. Evaluation of the pharmacokinetic profile of artesunate, artemether and their metabolites in sheep naturally infected with Fasciola hepatica. Vet Parasitol. 2012; 186 (3-4): 270-80. • Rinaldi L, Mihalca AD, Cirillo R, Maurelli MP, Montesano M, Capasso M, Cringoli G. FLOTAC can detect parasitic and pseudoparasit elements in reptiles. Exp Parasitol. 2012; 130(3): 282-4. • Steinmann P, Cringoli G, Bruschi F, Matthys B, Lohourignon LK, Castagna B, Maurelli MP, Morgoglione ME, Utzinger J, Rinaldi L. FLOTAC for the diagnosis of Hymenolepis spp. infection: proof-of-concept and comparing diagnostic accuracy with other methods. Parasitol Res. 2012; 111(2):749-54. • Dipineto L, Capasso M, Maurelli MP, Russo TP, Pepe P, Capone G, Fioretti A, Cringoli G, Rinaldi L. Survey of co-infection by Salmonella and oxyurids in tortoises. BMC Vet Res. 2012; 8:69. • Levecke B, Rinaldi L, Charlier J, Maurelli MP, Bosco A, Vercruysse J, Cringoli G.The bias, accuracy and precision of faecal egg count reduction test results in cattle using McMaster, Cornell- Wisconsin and FLOTAC egg counting methods.Vet Parasitol. 2012;188(1-2):194-9. • Rinaldi L, Gonzales S, Guerrero J, Aquilera LC, Musella V, Genchi C, Cringoli G. A One-Health integrated approach to control fascioliasis in the Cajamarca valley of Perù. Geospat Health. 2012, 6(3): S67-73. • Trapani F, Paciello O, Papparella S, Rinaldi L, Cringoli G, Maiolino P. Histopathological, histochemical and immunohistochemical findings of the small intestine in goats naturally infected by Trichostrongylus colubriformis.Vet Parasitol. 2013, 191(3-4):390-3. • Catelan D, Rinaldi L, Musella V, Cringoli G, Biggeri A. Statistical approaches for farm and parasitic risk profiling in geographical veterinary epidemiology.Stat Methods Med Res. 2012, 21(5): 531-43. • Rinaldi L, Capasso M, Mihalca AD, Cirillo R, Cringoli G, Cacciò S. Prevalence and molecular identification of Cryptosporidium isolates from pet lizards and snakes in Italy.Parasite. 2012,19(4):437-40. • Rinaldi L, Maurelli MP, Musella V, Bosco A, Cortes H, Cringoli G. First cross-sectional serological survey on Besnoitia besnoiti in cattle in Italy.Parasitol Res. 2013,112(4):1805-7. • Silva LMR, Vila-Viscosa MJM, Maurelli MP, Morgoglione ME, Cortes HCE, Cringoli G, Rinaldi L. Mini-FLOTAC for the diagnosis of Eimeria infection in goats: An alternative to McMaster. Small Ruminant Research 2013, 114: 280-283. • Demeler J, Ramünke S, Wolken S, Ianiello D, Rinaldi L, Gahutu JB, Cringoli G, von Samson- Himmelstjerna G, Krücken J. Discrimination of gastrointestinal nematode eggs from crude fecal egg preparations by inhibitor-resistant conventional and real-time PCR.PLoS One2013,8(4):e61285. SRTP Proposal Naples page 55 of 105

• Dipineto L,Rinaldi L,Bosco A,Russo TP,Fioretti A,Cringoli G. Co-infection by Escherichia coli O157 and gastrointestinal strongyles in sheep. Vet J.2013, 197(3):884-5. • Cringoli G, Rinaldi L, Albonico M, Bergquist R, Utzinger J. Geospatial (s)tools: integration of advanced epidemiological sampling and novel diagnostics.Geospat Health. 2013, 7(2):399-404. • Albonico M, Rinaldi L, Sciascia S, Morgoglione ME, Piemonte M, Maurelli MP, Musella V, Utzinger J, Ali SM, Ame SM, Cringoli G. Comparison of three copromicroscopic methods to assess albendazole efficacy against soil-transmitted helminth infections in school-aged children on Pemba island. Trans R Soc Trop Med Hyg. 2013, 107(8):493-501. • Barda B, Zepherine H, Rinaldi L, Cringoli G, Burioni R, Clementi M, Albonico M. Mini-FLOTAC and Kato-Katz: helminth eggs watching on the shore of lake Victoria. Parasit Vectors. 2013,6(1):220. • Barda BD, Rinaldi L, Ianniello D, Zepherine H, Salvo F, Sadutshang T, Cringoli G, Clementi M, Albonico M. Mini-FLOTAC, an Innovative Direct Diagnostic Technique for Intestinal Parasitic Infections: Experience from the Field.PLoS Negl Trop Dis. 2013, 7(8):e2344. • Meister I, Duthaler U, Huwyler J, Rinaldi L, Bosco A, Cringoli G, Keiser J.Efficacy and pharmacokinetics of OZ78 and MT04 against a natural infection with Fasciola hepatica in sheep.Vet Parasitol 2013,198(1-2):102-10. • Barda B, Ianniello D, Salvo F, Sadutshang T, Rinaldi L, Cringoli G, Burioni R, Albonico M."Freezing" parasites in pre-Himalayan region, Himachal Pradesh: Experience with mini- FLOTAC. Acta Trop. 2013; 130C:11-16. • Cuervo PF, Sierra RM, Waisman V, Gerbeno L, Sidoti L, Albonico F, Mariconti M, Mortarino M, Pepe P, Cringoli G, Genchi C, Rinaldi L. Detection of Dirofilaria immitis in mid-western arid Argentina. Acta Parasitol 2013; 58(4): 612-14. • Cuervo PF, Fantozzi MC, Di Cataldo S, Cringoli G, Mera y Sierra R, Rinaldi L. Analysis of climate and extrinsic incubation of Dirofilaria immitis in southern South America. Geospatial Health 2013;8: 175-81. • Bazzocchi C,Mariconti M,Sassera D,Rinaldi L,Martin E,Cringoli G,Urbanelli S,Genchi C,Bandi C,Epis S. Molecular and serological evidence for the circulation of the tick symbiont Midichloria (Rickettsiales: Midichloriaceae) in different mammalian species. Parasit Vectors.2013; 6(1): 350. • d'Ovidio D,Rinaldi L,Ianniello D,Donnelly TM,Pepe P,Capasso M,Cringoli G. FLOTAC for diagnosis of endo-parasites in pet squirrels in southern Italy. Vet Parasitol.2014, 200(1-2):221-4. • Dipineto L, Borrelli L, Pepe P, Fioretti A, Caputo V, Cringoli G, Rinaldi L. Synanthropic birds and parasites. Avian Dis. 2013, 57(4):756-8. • Rinaldi L, Morgan ER, Bosco A, Coles GC, Cringoli G. The maintenance of anthelmintic efficacy in sheep in a Mediterranean climate.Vet Parasitol. 2014, 203(1-2):139-43. • Rinaldi L, CringoliG. Exploring the interface between diagnostics and maps of neglected parasitic diseases.Parasitology 2014, 28:1-8. • Bosco A, Rinaldi L, Musella V, Pintus D, Santaniello M, Morgoglione ME, Zacometti G, Cringoli G. Helminths in Sheep on Farms of the Basilicata Region of Southern Italy. Trends in Veterinary Sciences 2013, p 91-94. • d'Ovidio D, Pepe P, Ianniello D, Noviello E, Quinton JF, Cringoli G, Rinaldi L.First survey of endoparasites in pet ferrets in Italy.Vet Parasitol. 2014, 203(1-2):227-30. • Maesano G, Capasso M, Ianniello D, Cringoli G, Rinaldi L.Parasitic infections detected by FLOTAC in zoo mammals from Warsaw, Poland.Acta Parasitol. 2014, 59(2):343-53. SRTP Proposal Naples page 56 of 105

• Barda B, Ianniello D, Zepheryne H, Rinaldi L, CringoliG, Burioni R, Albonico M. Parasitic infections on the shore of Lake Victoria (East Africa) detected by Mini-FLOTAC and standard techniques.Acta Trop. 2014;137:140-6. • Djokic V, Klun I, Musella V, Rinaldi L, Cringoli G, Sotiraki S, Djurkovic-Djakovic O. Spatial epidemiology of Toxoplasma gondii infection in goats in Serbia.Geospat Health. 2014, 8(2):479-88. • Barda B, Cajal P, Villagran E, Cimino R, Juarez M, Krolewiecki A, Rinaldi L, CringoliG, Burioni R, Albonico M.Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north Argentina.Parasit Vectors. 2014; 7(1):271. • Beugnet F,Bourdeau P,Chalvet-Monfray K,Cozma V,Farkas R,Guillot J,Halos L,Joachim A,Losson B,Miró G,Otranto D,Renaud M,Rinaldi L. Parasites of domestic owned cats in Europe: co- infestations and risk factors.Parasit Vectors.2014;7(1):291. • Rinaldi L, Levecke B, Bosco A, Ianniello D, Pepe P, Charlier J, Cringoli G, Vercruysse J.Comparison of individual and pooled faecal samples in sheep for the assessment of gastrointestinal strongyle infection intensity and anthelmintic drug efficacy using McMaster and Mini-FLOTAC. Vet Parasitol. 2014; 205(1-2):216-23. • Maurelli MP, Rinaldi L, Alfano S, Pepe P, Coles GC, Cringoli G. Mini-FLOTAC, a new tool for copromicroscopic diagnosis of common intestinal nematodes in dogs. Parasit Vectors. 2014; 7:356. • Musella V, Rinaldi L, Lagazio C, Cringoli G, Biggeri A, Catelan D. On the use of posterior predictive probabilities and prediction uncertainty to tailor informative sampling for parasitological surveillance in livestock.Vet Parasitol. 2014; 205(1-2):158-68. • Steinmann P, Rinaldi L, Cringoli G, Du ZW, Marti H, Jiang JY, Zhou H, Zhou XN, Utzinger J.Morphological diversity of Trichuris spp. eggs observed during an anthelminthic drug trial in Yunnan, China, and relative performance of parasitologic diagnostic tools. Acta Trop. 2014; S0001- 706X(14)00279-4. • Maurelli MP,RinaldiL, Rubino G, Lia R, Musella V,CringoliG.FLOTAC and Mini-FLOTAC for uro-microscopic diagnosis of Capillaria plica (syn. Pearsonema plica) in dogs.BMC Res Notes. 2014; 7(1):591. • Bosco A, Rinaldi L, Maurelli MP, Musella V, Coles GC, Cringoli G. The comparison of FLOTAC, Fecpak and McMaster techniques for nematode egg counts in cattle. Acta Parasitol. 2014;59(4):625-8. • Rinaldi L, Cortese L, Meomartino L, Pagano TB, Pepe P, Cringoli G, Papparella S. Angiostrongylus vasorum: epidemiological, clinical and histopathological insights. BMC Vet Res. 2014;10(1):236. • Djokic V, Blaga R, Rinaldi L, Le Roux D, Ducry T, Maurelli MP, Perret C, Djurkovic Djakovic O,Cringoli G, Boireau P.Mini-FLOTAC for counting Toxoplasma gondii oocysts from cat feces - Comparison with cell counting plates.Exp Parasitol. 2014;147C:67-71. • Mitrea IL, Ionita M, Costin II, Predoi G, Avram E, Rinaldi L, Maurelli MP, Cringoli G, Genchi C.Occurrence and genetic characterization of Echinococcus granulosus in naturally infected adult sheep and cattle in Romania.Vet Parasitol. 2014;206(3-4):159-166. • Neves JH, Carvalho N, Rinaldi L, Cringoli G, Amarante AF. Diagnosis of anthelmintic resistance in cattle in Brazil: A comparison of different methodologies.Vet Parasitol. 2014;206(3-4):216-226. • Godber OF, Phythian CJ, Bosco A, Ianniello D, Coles G, Rinaldi L, Cringoli G. A comparison of the FECPAK and Mini-FLOTAC faecal egg counting techniques.Vet Parasitol. 2014; 207(3-4):342- 5. SRTP Proposal Naples page 57 of 105

• Rinaldi L, Pennacchio S, Musella V, Maurelli M, La Torre F, Cringoli G. Helminth control in kennels: is the combination of milbemycin oxime and praziquantel a right choice? Parasit Vectors. 2015; 8(1):30. • d'Ovidio D, Noviello E, Ianniello D, Cringoli G, Rinaldi L. Survey of endoparasites in pet guinea pigs in Italy.Parasitol Res. 2015; 114(3):1213-6. • Cuervo PF, Rinaldi L, Cringoli G. Modeling the extrinsic incubation of Dirofilaria immitis in South America based on monthly and continuous climatic data. Vet Parasitol. 2015; 209(1-2):70-5. • Rinaldi L, Biggeri A, Musella V, De Waal T, Hertzberg H, Mavrot F, Torgerson PR, Selemetas N, Coll T, Bosco A, Grisotto L, Cringoli G, Catelan D. Sheep and Fasciola hepatica in Europe: the GLOWORM experience. Geospat Health. 2015; 9(2):309-317. • Bosco A, Rinaldi L, Musella V, Amadesi A, Cringoli G. Outbreak of acute fasciolosis in sheep farms in a Mediterranean area arising as a possible consequence of climate change.Geospat Health. 2015; 9(2):319-324. • Rinaldi L, Hendrickx G, Cringoli G, Biggeri A, Ducheyne E, Catelan D, Morgan E, Williams D, Charlier J, Von Samson-Himmelstjerna G, Vercruysse J.Mapping and modelling helminth infections in ruminants in Europe: experience from GLOWORM.Geospat Health. 2015;9(2):257- 259. • Rinaldi L, Catelan D, Musella V, Cecconi L, Hertzberg H, Torgerson PR, Mavrot F, De Waal T, Selemetas N, Coll T, Bosco A, Biggeri A, Cringoli G. Haemonchus contortus: spatial risk distribution for infection in sheep in Europe.Geospat Health. 2015;9(2):325-331. • Bolajoko MB, Rose H, Musella V, Bosco A, Rinaldi L, Van Dijk J, Cringoli G, Morgan ER.The basic reproduction quotient (Q0) as a potential spatial predictor of the seasonality of ovine haemonchosis.Geospat Health. 2015;9(2):333-350. • Ducheyne E, Charlier J, Vercruysse J, Rinaldi L, Biggeri A, Demeler J, Brandt C, De Waal T, Selemetas N, Höglund J, Kaba J, Kowalczyk SJ, Hendrickx G.Modelling the spatial distribution of Fasciola hepatica in dairy cattle in Europe.Geospat Health. 2015;9(2):261-270. • Barda B, Albonico M, Ianniello D, Ame SM, Keiser J, Speich B, Rinaldi L, Cringoli G, Burioni R, Montresor A, Utzinger J. How Long Can Stool Samples Be Fixed for an Accurate Diagnosis of Soil-Transmitted Helminth Infection Using Mini-FLOTAC?PLoS Negl Trop Dis. 2015; 9(4):e0003698. • Lima V.F., Cringoli G., Rinaldi L., Monteiro M.F., Calado A.M., Ramos R.A., Meira-Santos P.O., Alves L.C. A comparison of mini-FLOTAC and FLOTAC with classic methods to diagnosing intestinal parasites of dogs from Brazil. Parasitol Res. 2015; 114(9):3529-33. • Bosco A., Rinaldi L., Cappelli G., Saratsis A., Nisoli L., Cringoli G. Metaphylactic treatment strategies with toltrazuril and diclazuril and growth performance of buffalo calves exposed to a natural eimeria infection.Vet Parasitol. 2015;212(3-4):408-10. • Pepe P., Castellano M., Alfano S., Della Pepa M.E., Tirino V., Piemonte M., Desiderio V., Zappavigna S., Galdiero M., Caraglia M., Cringoli G., Rinaldi L., Galdiero M. Dicrocoelium dendriticum induces autophagic vacuoles accumulation in human hepatocarcinoma cells.Vet Parasitol. 2015; 212(3-4):175-80. • d'Ovidio D., Noviello E., Pepe P., Del Prete L., Cringoli G., Rinaldi L. Survey of Hymenolepis spp. in pet rodents in Italy.Parasitol Res. 2015; 114(12):4381-4. • Borrelli L., Dipineto L., Rinaldi L., Romano V., Noviello E., Menna L.F., Cringoli G., Fioretti A. New diagnostic insights for Macrorhabdus ornithogaster infection.J Clin Microbiol.2015; SRTP Proposal Naples page 58 of 105

53(11):3448-50.Ramos RA, Lima VF, Monteiro MF, de Andrade Santana M, Lepold R, Faustino MA, Rinaldi L, Cringoli G, Alves LC. New insights into diagnosis of Platynosomum fastosum (Trematoda: Dicrocoeliidae) in cats.Parasitol Res. 2016; 115(2):479-82. • Tudor P, Turcitu M, Mateescu C, Dantas-Torres F, Tudor N, Bărbuceanu F, Ciuca L, Burcoveanu I, Acatrinei D, Rinaldi L, Mateescu R, Bădicu A, Ionașcu I, Otranto D. Zoonotic ocular onchocercosis caused by Onchocerca lupi in dogs in Romania.Parasitol Res. 2016; 115(2):859-62. • Del Prete L, Maurelli MP, Pennacchio S, Bosco A, Musella V, Ciuca L, Cringoli G, Rinaldi L. Dirofilaria immitis and Angiostrongylus vasorum: the contemporaneous detection in kennels. BMC Vet Res. 2015; 11(1):305. • Rose H, Rinaldi L, Bosco A, Mavrot F, de Waal T, Skuce P, Charlier J, Torgerson PR, Hertzberg H, Hendrickx G, Vercruysse J, Morgan ER. Widespread anthelmintic resistance in European farmed ruminants: a systematic review. Vet Rec. 2015;176(21):546. • Gualdieri L, Piemonte M, Alfano S, Maffei R, Della Pepa ME, Rinaldi L, Galdiero M, Galdiero M, Cringoli G. Immigrants living in an urban milieu with sanitation in Southern Italy: persistence and transmission of intestinal parasites. Parasitol Res. 2016; 115(3):1315-23. • Lima VF, Ramos RA, Lepold R, Cringoli G, Rinaldi L, Faustino MA, Alves LC. Use of the FLOTAC technique to diagnosing parasites of the urinary tract of dogs. Parasitol Res. 2016; 115(4):1737-9. • Cecconi L, Biggeri A, Grisotto L, Berrocal V, Rinaldi L, Musella V, Cringoli G, Catelan D. Preferential sampling in veterinary parasitological surveillance. Geospat Health. 2016; 11(1):412. • Condoleo R, Musella V, Maurelli MP, Bosco A, Cringoli G, Rinaldi L. Mapping, cluster detection and evaluation of risk factors of ovine toxoplasmosis in Southern Italy. Geospat Health. 2016, 11(2):432. • Kenyon F, Rinaldi L, McBean D, Pepe P, Bosco A, Melville L, Devin L, Mitchell G, Ianniello D, Charlier J, Vercruysse J, Cringoli G, Levecke B. Pooling sheep faecal samples for the assessment of anthelmintic drug efficacy using McMaster and Mini-FLOTAC in gastrointestinal strongyle and Nematodirus infection. Vet Parasitol. 2016, 225:53-60. • Ciucă L, Genchi M, Kramer L, Mangia C, Miron LD, Prete LD, Maurelli MP, Cringoli G, Rinaldi L. Heat treatment of serum samples from stray dogs naturally exposed to Dirofilaria immitis and Dirofilaria repens in Romania. Vet Parasitol. 2016, 225:81-5. • Costagliola A, Piegari G, Otrocka-Domagala I, Ciccarelli D, Iovane V, Oliva G, Russo V, Rinaldi L, Papparella S, Paciello O. Immunopathological Features of Canine Myocarditis Associated with Leishmania infantum Infection. Biomed Res Int. 2016;2016:8016186. • Coulibaly JT, Ouattara M, Becker SL, Lo NC, Keiser J, N'Goran EK, Ianniello D, Rinaldi L, Cringoli G, Utzinger J. Comparison of sensitivity and faecal egg counts of Mini-FLOTAC using fixed stool samples and Kato-Katz technique for the diagnosis of Schistosoma mansoni and soil- transmitted helminths. Acta Trop. 2016. S0001-706X(16)30661-1. • Chaligiannis I, Musella V, Rinaldi L, Cringoli G, de la Fuente J, Papa A, Sotiraki S. Species diversity and spatial distribution of ixodid ticks on small ruminants in Greece. Parasitol Res. 2016 Sep 22. [Epub ahead of print]. • Rose Vineer H, Steiner J, Knapp-Lawitzke F, Bull K, von Son-de Fernex E, Bosco A, Hertzberg H, Demeler J, Rinaldi L, Morrison AA, Skuce P, Bartley DJ, Morgan ER. Implications of between- isolate variation for climate change impact modelling of Haemonchus contortus populations. Vet Parasitol. 2016 229:144-149. SRTP Proposal Naples page 59 of 105

• Ramünke S, Melville L, Rinaldi L, Hertzberg H, de Waal T, von Samson-Himmelstjerna G, Cringoli G, Mavrot F, Skuce P, Krücken J, Demeler J. Benzimidazole resistance survey for Haemonchus, Teladorsagia and Trichostrongylus in three European countries using pyrosequencing including the development of new assays for Trichostrongylus. Int J Parasitol Drugs Drug Resist. 2016, 6(3):230-240. • Ciucă L, Musella V, Miron LD, Maurelli MP, Cringoli G, Bosco A, Rinaldi L. Geographic distribution of canine heartworm (Dirofilaria immitis) infection in stray dogs of eastern Romania. Geospat Health. 2016, 11(3):499.