DOCSLIB.ORG

Lepidoptera: Nymphalidae)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Lepidoptera: Nymphalidae) View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Repository of the Academy's Library Biological Journal of the Linnean Society, 2017, XX, 1–15. With 5 figures. Mito-nuclear discordance helps to reveal the phylogeographic patterns of Melitaea ornata (Lepidoptera: Nymphalidae) JÁNOS P. TÓTH1,*, ZOLTÁN VARGA2, RUDI VEROVNIK3, NIKLAS WAHLBERG4, ALEX VÁRADI2 and JUDIT BERECZKI2 1MTA-DE ‘Lendület’ Behavioural Ecology Research Group, 4032 Debrecen Egyetem tér 1, Hungary 2Department of Evolutionary Zoology and Human Biology, Institute of Biology and Ecology, University of Debrecen, 4032 Debrecen Egyetem tér 1, Hungary 3Department of Biology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia 4Department of Biology, Lund University, 223 62 Lund, Sweden Received 6 September 2016; revised 7 November 2016; accepted for publication 1 December 2016 Periodical changes of glacial and interglacial conditions have influenced the distribution of most living organisms and shape the separation of different genetic lineages significantly. We investigated the phylogeography of a nym- phalid butterfly Melitaea ornata. Our main aim was to explore the existence, the origin, and the variability of differ- ent genetic lineages based on a multilevel approach. M. ornata and its close relatives (with a focus on M. phoebe) from the Palearctic were analysed based on five gene regions (COI, EF-1a, MDH, RPS5, and wingless) using Bayesian methods to infer the phylogeographic history. The DNA-based analyses have been complemented with species dis- tribution modelling (SDM) and Wolbachia screening. The Bayesian inference analysis showed mito-nuclear discord- ance in M. ornata, which is split into an eastern and a western clade. Based on mitochondrial DNA , the western clade of M. ornata clusters together with M. phoebe, while the eastern clade is well-separated. In contrast to this, the combined nuDNA-based analysis revealed that M. ornata forms a monophyletic group which is clearly separated from M. phoebe. The timing of divergence analyses suggest that the split between M. ornata and M. phoebe is about 6 million years old based on both the COI and the concatenated nuclear genes. SDM predicted considerably larger area shifts for M. phoebe than for M. ornata. LGM refugia were predicted for both species to the Mediterranean pen- insulas in Europe and several Middle-East and Asian localities. The prevalence of Wolbachia infection was 88.9% in M. phoebe and only 7% in M. ornata. Our results clearly indicate a lack of ongoing hybridization between M. phoebe and M. ornata, but argue for an ancient hybridization event in the Apennine Peninsula which strongly influenced the observed split between the two clades of M. ornata. ADDITIONAL KEYWORDS: hybridization – Last Glacial Maximum – species distribution modelling – Wolbachia. INTRODUCTION of Earth’s climate sensitivity to CO2-based radiative forcing which was half as strong during the warm The Earth’s climate has shown a general cooling Pliocene as during the cold late Pleistocene epoch (0.8– trend that began around 45 Mya in the Eocene with 0.01 Mya) (Lisiecki & Raymo, 2007; Martínez-Botí strong oscillations from the Miocene (Zachos, Dickens et al., 2015). These periodical changes of glacial and & Zeebe, 2008). Various records from the Quaternary interglacial conditions have influenced the distribu- show increasingly severe ice age cycles with shorter tion of most living organisms significantly. According and shorter warm interglacials driven by the change to the classic refugia paradigm for Europe, the bulk of temperate species could survive in southern Europe *Corresponding author. E-mail: [email protected] during the glaciations, mostly in climatically buffered © 2017 The Linnean Society of London, Biological Journal of the Linnean Society, 2017, XX, 1–15 1 2 J. P. TÓTH ET AL. parts of the Mediterranean peninsulas (de Lattin, mtDNA-based analyses could be misleading. Thus, a 1967; Hewitt, 1996; Schmitt, 2007), although this phylogeographic or a phylogenetic study using only paradigm has been greatly refined by the growing mtDNA is unreliable without Wolbachia screening. amount of new information. Past distributions of spe- Multiple gene analyses are strongly recommended cies are essentially inferred from four types of evi- not only for phylogeography but also for phyloge- dence (Tzedakis, Emerson & Hewitt, 2013): (1) plant netic studies in general (e.g. Wiemers & Fiedler, 2007; and animal macrofossils; (2) pollen records; (3) genetic Wahlberg et al., 2009). Moreover, the combined use of data; and (4) species distribution models. different data sets and methods – for example mul- The most comprehensive coverage comes from plant tiple gene analyses complemented with Wolbachia macrofossils and pollen spectra. These indicate that screening, morphometric data, and species distribu- many species respond individually to climatic oscil- tion modelling (SDM) predictions – enables us to bet- lations (Bhagwat & Willis, 2008; Stewart et al., 2010) ter understand how the various factors together shape depending on differential responses to various ecological the evolutionary relationships among taxa. factors. These species-specific reactions produce a vari- SDM could be useful to estimate the potential dis- ety of distributional patterns by speeding up or slowing tribution at different time scales, thus it could provide down range expansion and contraction, thus they can insight into area dynamics even for fossil-deficient result in a significant difference in species composition species. Therefore, SDM has become an important of communities that could be essentially different from tool for historical biogeography usually combined with those observed today (Schmitt & Varga, 2012). Obviously, molecular methods or morphometry (e.g. Habel et al., the change of vegetation has also an effect on the animal 2011; Schorr et al., 2012; Rajaei et al., 2013; Zinetti assemblages, although the fossil record is poor or not et al., 2013; Tóth, Varga & Bereczki, 2016). available for the majority of animal species, especially The demand for such multilevel approach has arisen for insects. For these fossil-deficient groups, molecular in the case of the Melitaea phoebe species group, which methods and distribution modelling techniques could has been studied intensively in the last few years. It help us to infer their biogeographic history. The disjunct became clear that a cryptic species, namely M. ornata glacial distribution patterns have regularly resulted in Christoph, 1893, was concealed within the morphologi- the separation of different genetic lineages in the three cally similar species M. phoebe from which it was first major Mediterranean peninsulas, Anatolia (Comes distinguished based on larval morphology (Russell & Kadereit, 1998; Taberlet et al., 1998; Hewitt, 1999, et al., 2005; Varga, Szabó & Kozma, 2005). 2000; Schmitt, 2007), North Africa, large Mediterranean M. phoebe ([Denis & Schiffermüller, 1775]) is dis- islands, and also some microrefugial areas (Provan & tributed widely in the Palearctic, although it seems Bennett, 2008; Dapporto, 2010; Husemann et al., 2014). less abundant under Mediterranean climate condi- Mitochondrial DNA (mtDNA) proved to be suitable tions. M. ornata also has a relatively large distribu- for barcoding animal life and highly informative in tion but the most northern localities are found in the phylogenetic and phylogeographic studies (Hebert, Carpathian Basin and in the Volgograd Region in Ratnasingham & de Waard, 2003). Nevertheless, the Russia (Kuznetsov & Stradomsky, 2010). Owing to the use of nuclear (nuDNA) markers in addition to mtDNA application of geometric morphometrics and phyloge- improves the power of phylogenetic and phylogeo- netic methods, two more Melitaea species have been graphic hypothesis tests significantly and highlights identified in this group, namely M. telona Fruhstorfer, the limitations of studies using only mtDNA markers. 1908 and M. abbas Gross & Ebert, 1975, and the Results based solely on mtDNA could be very differ- relationships between these taxa have become much ent from those which are inferred from nuDNA (e.g. clearer through a series of recent studies (Leneveu, Wahlberg et al., 2009); a phenomenon known as mito- Chichvarkhin & Wahlberg, 2009; Tóth & Varga, 2011; nuclear discordance. According to Toews & Brelsford Tóth et al., 2014, 2016). One of the most surprising (2012) the most frequent explanations of mito-nuclear discoveries was that M. telona from the Levant region discordance are incomplete lineage sorting, adaptive appears to be a distinct species also based on molecu- introgression of mtDNA, demographic disparities lar data (Tóth et al., 2014). Additionally, a more focused and sex-biased asymmetries, hybrid zone movement, study on Iranian Melitaea specimens (Tóth, et al., human introductions, and Wolbachia infection. 2016) from several localities showed that M. ornata is Recently, it has become clear that patterns of a rare species in this region and found exclusively in mtDNA inheritance could be highly manipulated the north-eastern part of the country, while the widely by the maternally inherited, intracellular bacteria distributed sister species in Iran is M. abbas. genus Wolbachia. This microorganism could induce The distribution of M. ornata at different time scales ‘two barcodes – one species’ (Kodandaramaiah et al., has already been studied using the Maximum Entropy
Load more

Recommended publications
  • Révision Taxinomique Et Nomenclaturale Des Rhopalocera Et Des Zygaenidae De France Métropolitaine
    Direction de la Recherche, de l’Expertise et de la Valorisation Direction Déléguée au Développement Durable, à la Conservation de la Nature et à l’Expertise Service du Patrimoine Naturel Dupont P, Luquet G. Chr., Demerges D., Drouet E. Révision taxinomique et nomenclaturale des Rhopalocera et des Zygaenidae de France métropolitaine. Conséquences sur l’acquisition et la gestion des données d’inventaire. Rapport SPN 2013 - 19 (Septembre 2013) Dupont (Pascal), Demerges (David), Drouet (Eric) et Luquet (Gérard Chr.). 2013. Révision systématique, taxinomique et nomenclaturale des Rhopalocera et des Zygaenidae de France métropolitaine. Conséquences sur l’acquisition et la gestion des données d’inventaire. Rapport MMNHN-SPN 2013 - 19, 201 p. Résumé : Les études de phylogénie moléculaire sur les Lépidoptères Rhopalocères et Zygènes sont de plus en plus nombreuses ces dernières années modifiant la systématique et la taxinomie de ces deux groupes. Une mise à jour complète est réalisée dans ce travail. Un cadre décisionnel a été élaboré pour les niveaux spécifiques et infra-spécifique avec une approche intégrative de la taxinomie. Ce cadre intégre notamment un aspect biogéographique en tenant compte des zones-refuges potentielles pour les espèces au cours du dernier maximum glaciaire. Cette démarche permet d’avoir une approche homogène pour le classement des taxa aux niveaux spécifiques et infra-spécifiques. Les conséquences pour l’acquisition des données dans le cadre d’un inventaire national sont développées. Summary : Studies on molecular phylogenies of Butterflies and Burnets have been increasingly frequent in the recent years, changing the systematics and taxonomy of these two groups. A full update has been performed in this work.
    [Show full text]
  • Russell & Tennent: a Synonymic List of Names Associated With
    ©Societas Europaea Lepidopterologica; download unter http://www.soceurlep.eu/ und www.zobodat.at Nota Lepi. 39(1) 2016: 27–56 | DOI 10.3897/nl.39.5929 A synonymic list of names associated with western Palaearctic Melitaea phoebe (Denis & Schiffermüller, 1775) species group taxa (M. phoebe; M. punica Oberthür, 1876; M. ornata Christoph, 1893) (Lepidoptera, Nymphalidae) Peter Russell1, W. John Tennent2 1 Oakmeadow, Wessex Avenue, East Wittering, West Sussex PO20 8NP, U.K.; [email protected] 2 Scientific Associate, Division of Insects, Department of Life Sciences, Natural History Museum, Cromwell Road, London SW7 5BD, U.K.; [email protected] http://zoobank.org/721CB232-8B92-4542-BF4D-563040229871 Received 17 August 2015; accepted 9 January 2016; published: 18 April 2016 Subject Editor: Thomas Schmitt. Abstract. Following indecision and confusion in the literature regarding nomenclature and distribution of Melitaea phoebe (Denis & Schiffermüller, 1775) and its closely associated congeners M. punica Oberthür, 1876, and M. ornata Christoph, 1893, a synonymic list of phoebe names, and of names both correctly and mis- takenly associated with phoebe species-group taxa, is presented. Explanatory footnotes provide the basis of a stable source for future discussion of M. phoebe species-group populations throughout the species’ ranges. Introduction According to a recent revision (van Oorschot and Coutsis 2014), the genus Melitaea Fabricius, 1807, comprises some 98 species of phenotypically similar, medium sized nymphaline butterflies that occur throughout most of the Palaearctic Region and beyond. Adults are invariably orange- brown on the upper surface, with a series of black lines and other markings; several Melitaea species are notoriously difficult to separate using wing morphology due to their similarity in appearance (Jugovic and Koren 2014).
    [Show full text]
  • Maquetación 1
    About IUCN IUCN is a membership Union composed of both government and civil society organisations. It harnesses the experience, resources and reach of its 1,300 Member organisations and the input of some 15,000 experts. IUCN is the global authority on the status of the natural world and the measures needed to safeguard it. www.iucn.org https://twitter.com/IUCN/ IUCN – The Species Survival Commission The Species Survival Commission (SSC) is the largest of IUCN’s six volunteer commissions with a global membership of more than 10,000 experts. SSC advises IUCN and its members on the wide range of technical and scientific aspects of species conservation and is dedicated to securing a future for biodiversity. SSC has significant input into the international agreements dealing with biodiversity conservation. http://www.iucn.org/theme/species/about/species-survival-commission-ssc IUCN – Global Species Programme The IUCN Species Programme supports the activities of the IUCN Species Survival Commission and individual Specialist Groups, as well as implementing global species conservation initiatives. It is an integral part of the IUCN Secretariat and is managed from IUCN’s international headquarters in Gland, Switzerland. The Species Programme includes a number of technical units covering Species Trade and Use, the IUCN Red List Unit, Freshwater Biodiversity Unit (all located in Cambridge, UK), the Global Biodiversity Assessment Initiative (located in Washington DC, USA), and the Marine Biodiversity Unit (located in Norfolk, Virginia, USA). www.iucn.org/species IUCN – Centre for Mediterranean Cooperation The Centre was opened in October 2001 with the core support of the Spanish Ministry of Agriculture, Fisheries and Environment, the regional Government of Junta de Andalucía and the Spanish Agency for International Development Cooperation (AECID).
    [Show full text]
  • Diversity and Ecology of Butterflies and Moths in Wadi Gaza, Gaza Strip, Palestine
    International Journal of Scientific and Research Publications, Volume 5, Issue 11, November 2015 707 ISSN 2250-3153 Diversity and Ecology of Butterflies and Moths in Wadi Gaza, Gaza strip, Palestine Zuhair .W. Dardona*, Ayman .W. Dardona**, Mohammed.A.Albayoumi * * Msc Microbiology ** Msc Limnology Abstract- Butterflies and moths were studied in regions of Wadi Gaza, extending from Salahe El-deen bridge west to Wadi Abo- Qatron near Wadi Gaza village to the east. The research is based on studying the diversity of butterflies and moths in terms of taxa diversity, Genera compositions, and family abundance. In terms of family abundance, the survey showed that all recorded butterflies are belonging to five main families (Pieridae, Hesperiidae, Lycaenidae, Nymphalidae, Papilionidae). The recorded moths are also belonging to five families (Arctiidae, Crambidae, Geometridae, Noctuidae, Sphingidae). In terms of species and genera compositions and diversity, the survey revealed that butterflies are more abundant concerning diversity and richness than moths. The five families of butterflies are consisting of (19 genera) and (25 species) while the five families of moths are consisting of only (10 genera) and (11species).The butterflies represented (69 %) of recorded species in the area of study while the moths were represented in (31 %) of the findings. The most abundant family of butterflies is Pieridae with (36%) of all recorded butterflies, followed by Lycaenidae (32%). As for moths, the abundant families are Noctuidae, Geometridae, and Crambidae were each family was represented by (3 species), and they form (82%) of recorded moths. In this study all genera, in both moths and butterflies are represented only by one specie except six genera of butterflies and one genus of moths as each one is represented with two species, these six genera of butterflies are zizeeria,Vanessa, Colias, Pieris, Carcharodus, and Pointa and that genues of moths is Stemorrhages.
    [Show full text]
  • Phylogeny of European Butterflies V1.0
    bioRxiv preprint doi: https://doi.org/10.1101/844175; this version posted November 16, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. A complete time-calibrated multi-gene phylogeny of the European butterflies Martin Wiemers1,2*, Nicolas Chazot3,4,5, Christopher W. Wheat6, Oliver Schweiger2, Niklas Wahlberg3 1Senckenberg Deutsches Entomologisches Institut, Eberswalder Straße 90, 15374 Müncheberg, Germany 2UFZ – Helmholtz Centre for Environmental Research, Department of Community Ecology, Theodor- Lieser-Str. 4, 06120 Halle, Germany 3Department of Biology, Lund University, 22362 Lund, Sweden 4Department of Biological and Environmental Sciences, University of Gothenburg, Box 461, 405 30 Gothenburg, Sweden. 5Gothenburg Global Biodiversity Centre, Box 461, 405 30 Gothenburg, Sweden. 6Department of Zoology, Stockholm University, 10691 Stockholm, Sweden *corresponding author: e-mail: [email protected] Abstract With the aim of supporting ecological analyses in butterflies, the third most species-rich superfamily of Lepidoptera, this paper presents the first time-calibrated phylogeny of all 496 extant butterfly species in Europe, including 18 very localized endemics for which no public DNA sequences had been available previously. It is based on a concatenated alignment of the mitochondrial gene COI and up to 11 nuclear gene fragments, using Bayesian inference of phylogeny. To avoid analytical biases that could result from our region-focus sampling, our European tree was grafted upon a global genus- level backbone butterfly phylogeny for analyses. In addition to a consensus tree, we provide the posterior distribution of trees and the fully-concatenated alignment for future analyses.
    [Show full text]
  • Acta Academiae Beregsasiensis 2012/2 1
    Acta Academiae Beregsasiensis 2012/2 1 Acta Academiae Beregsasiensis 2012/2 2 A II.Ráó k czi Ferenc Kááyő rp taljai M ag ar F iskola tudományos évkönyve Acta Academiae Beregsasiensis 2012/2 3 Acta Academiae Beregsasiensis A II. Rákóczi Ferenc Kárpátaljai Magyar Főiskola tudományos évkönyve Науковий вісник Закарпатського угорського інституту ім. Ф. Ракоці ІІ A Scholarly Annual of Ferenc Rákóczi II. Transcarpathian Hungarian Institute 2012 XI. évfolyam, 2. kötet Том XI, № 2 Volume XI, № 2 PoliPrint 2012 4 УДК 001.2 ББК 72 А-19 Az Acta Academiae Beregsasiensis a II. Rákóczi Ferenc Kárpátaljai Magyar Főiskola tudományos kiadványa. Jelen kötet a 2012-es év második felének magyar, orosz és angol nyelvű tanulmányait foglalja magába. Az intézmény tanárainak, hallgatóinak, valamint külföldi tudósok munkáit publikáló kötet a nyelvészet, irodalom, biológia, történelem, turizmus és informatika tudományágainak különböző területeit öleli fel. www.kmf.uz.ua/hun114/index.php/kiadvanyaink/110-a-ii-rakoczi-ferenc-karpataljai-magyar-fiskola-tudomanyos-evkoenyve SZERKE S ZTÉ S : Kohut Attila, Penckófer János KORREKTÚRA : G. Varcaba Ildikó TÖRDELÉ S : Kohut Attila BORÍTÓ : K&P A KIADÁ S ÉRT FELEL : dr. Orosz Ildikó, dr. Szikura József A k ö t e t t A n u l m á n y A i b A n e l ő f o r d u l ó állításokért m i n d e n e s e t b e n A s z e r z ő f e l e l . A kiadvány megjelenését a támogatta Készült: PoliPrint Kft., Ungvár, Turgenyev u. 2. Felelős vezető: Kovács Dezső ISBN: 978-966-2595-29-1 © A szerzők, 2012 Acta Academiae Beregsasiensis 2012/2 5 Tartalom * PÉC S I ANDREA : A II.RFKMF hallgatóinak nyelvhasználata kérdőíves felmérés alapján .........................................................................................
    [Show full text]
  • Lepidoptera: Nymphalidae): New Insights from Molecular and Morphometric Information
    Relationships within the Melitaea phoebe species group (Lepidoptera: Nymphalidae): new insights from molecular and morphometric information János P. Tóth1,2, Judit Bereczki1,3, Zoltán Varga1, Jadranka Rota6, Gábor Sramkó4,5, Niklas Wahlberg6 1Department of Evolutionary Zoology & Human Biology, Institute of Biology & Ecology, University of Debrecen, Debrecen, Hungary; 2Research Institute for Viticulture and Oenology, Tarcal, Hungary; 3MTA-DE „Lendület‟ Behavioural Ecology Research Group, University of Debrecen, Debrecen, Hungary; 4MTA-ELTE-MTM Ecology Research Group, Budapest, Hungary; 5Department of Botany, Institute of Biology & Ecology, University of Debrecen, Debrecen, Hungary; 6Laboratory of Genetics, Department of Biology, University of Turku, Turku, Finland Correspondence: János P. Tóth, Department of Evolutionary Zoology & Human Biology, Institute of Biology & Ecology, University of Debrecen, Debrecen, P.O.Box 3, H-4010, Hungary. E-mail: [email protected] Abstract The genus Melitaea consists of about 80 species, divided into 10 species groups, which are all restricted to the Palaearctic region. The Melitaea phoebe group was defined by Higgins based on morphological characters such as wing pattern and genital structures. According to his interpretation, the M. phoebe group included seven species: M. phoebe, M. sibina, M. scotosia, M. aetherie, M. collina, M. consulis and M. turkmanica. The taxonomy of the phoebe species group has been poorly resolved and recent results on the species composition within the group suggest the need for a re-evaluation. In this study molecular sequences (5985 bp) including one mitochondrial (COI) and up to six nuclear (CAD, EF-1α , GAPDH, MDH, RpS5 and wingless) gene regions from 38 specimens of the Melitaea phoebe species group sensu Higgins and some closely related taxa from the Palaearctic region were analysed.
    [Show full text]
  • Phylogeny of European Butterflies V1.0
    bioRxiv preprint doi: https://doi.org/10.1101/844175; this version posted November 16, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. A complete time-calibrated multi-gene phylogeny of the European butterflies Martin Wiemers1,2*, Nicolas Chazot3,4,5, Christopher W. Wheat6, Oliver Schweiger2, Niklas Wahlberg3 1Senckenberg Deutsches Entomologisches Institut, Eberswalder Straße 90, 15374 Müncheberg, Germany 2UFZ – Helmholtz Centre for Environmental Research, Department of Community Ecology, Theodor- Lieser-Str. 4, 06120 Halle, Germany 3Department of Biology, Lund University, 22362 Lund, Sweden 4Department of Biological and Environmental Sciences, University of Gothenburg, Box 461, 405 30 Gothenburg, Sweden. 5Gothenburg Global Biodiversity Centre, Box 461, 405 30 Gothenburg, Sweden. 6Department of Zoology, Stockholm University, 10691 Stockholm, Sweden *corresponding author: e-mail: [email protected] Abstract With the aim of supporting ecological analyses in butterflies, the third most species-rich superfamily of Lepidoptera, this paper presents the first time-calibrated phylogeny of all 496 extant butterfly species in Europe, including 18 very localized endemics for which no public DNA sequences had been available previously. It is based on a concatenated alignment of the mitochondrial gene COI and up to 11 nuclear gene fragments, using Bayesian inference of phylogeny. To avoid analytical biases that could result from our region-focus sampling, our European tree was grafted upon a global genus- level backbone butterfly phylogeny for analyses. In addition to a consensus tree, we provide the posterior distribution of trees and the fully-concatenated alignment for future analyses.
    [Show full text]
  • Vol. 9, No.1, 2019
    UNIVERSITY OF PRISTINA-FACULTY OF SCIENCES KOSOVSKA MITROVICA-REPUBLIC OF SERBIA VOL. 9, No 1, 2019. _______________________________________________________________________ ISSN 1450-7226 (Print) ISSN 2560-3094 (Online) UNIVERSITY THOUGHT-PUBLICATION IN NATURAL SCIENCES Published by University of Pristina-Faculty of Sciences Kosovska Mitrovica-Republic of Serbia Aims and Scope The University Thought - Publication in Natural Sciences (Univ. thought, Publ. nat. sci.) is a scientific journal founded in 1994. by the University of Priština, and was published semi annually until 1998. Today, the University Thought - Publication in Natural Sciences is an international, peer reviewed, Open Access journal, published semi annually in the online and print version by the University of Priština, temporarily settled in Kosovska Mitrovica, Serbia. The Journal publishes articles on all aspects of research in Biology, Chemistry, Geography, Information technologies, Mathematics and Physics in the form of original papers, short communications and reviews (invited) by authors from the country and abroad. The University Thought - Publication in Natural Sciences serves as an interdisciplinary forum covering a wide range of topics for a truly international audience. Journal is endeavor of the University of Priština to acquaint the scientific world with its achievements and wish to affirm the intellectual potential and natural resources of own region. Our aim is to put forward attitude of principle that science is universal and we invite all scientists to cooperate wherever their scope of research may be. We are convinced that shall contribute to do victory of science over barriers of all kinds erected throughout the Balkans. Directors Zdravko K. Vitošević and Nebojša V. Ţivić Editor in Chief Nebojša V.
    [Show full text]
  • Two Consecutive Wolbachia‐Mediated Mitochondrial Introgressions Obscure Taxonomy in Palearctic Swallowtail Butterflies (Lepidoptera, Papilionidae)
    Received: 17 January 2019 | Revised: 17 April 2019 | Accepted: 19 April 2019 DOI: 10.1111/zsc.12355 ORIGINAL ARTICLE Two consecutive Wolbachia‐mediated mitochondrial introgressions obscure taxonomy in Palearctic swallowtail butterflies (Lepidoptera, Papilionidae) Aurélien Gaunet1 | Vlad Dincă2 | Leonardo Dapporto3 | Sergio Montagud4 | Raluca Vodă5 | Sämi Schär1 | Arnaud Badiane6,7 | Enrique Font7 | Roger Vila1 1Institute of Evolutionary Biology (CSIC- UPF), Barcelona, Spain Abstract 2Department of Ecology and Swallowtail butterflies (Papilionidae) are among the most spectacular and well‐ Genetics, University of Oulu, Oulu, Finland known Lepidoptera in the European fauna, but their systematics is not fully elu- 3 Dipartimento di Biologia, Università degli cidated. A notable case is that of Iphiclides feisthamelii which, after more than Studi di Firenze, Florence, Italy 4 180 years since description, still has a debated status, being often considered as a Museu [UV] Història Natural, Universitat Iphiclides podalirius de València, Burjassot (Valencia), Spain subspecies of . To elucidate the relationship between the two 5Dipartimento di Scienze della Vita e taxa and the evolutionary processes that led to their separation, we combine mi- Biologia dei Sistemi, Università degli Studi tochondrial and nuclear DNA (mtDNA and nDNA) data, Wolbachia screening, di Torino, Turin, Italy genitalia morphology and wing UV reflectance. Our results show that the two taxa 6Department of Biological clearly differ in male and female genital morphology, male wing UV reflectance Sciences, Macquarie University, Sydney, New South Wales, Australia and nDNA. Two Wolbachia strains were found to widely infect the studied sam- 7Ethology Lab, Cavanilles Institute ples, apparently explaining the phylogeographic pattern displayed by mtDNA. The of Biodiversity and Evolutionary available data point towards a historical Wolbachia infection that spread from I.
    [Show full text]
  • ACTA BIANCO 1 2014.Qxp
    ACTA ENTOMO LOGICA SL OVENICA LJUBLJANA, DECEMBER 2020 Vol. 28, øt. 2: 159 –164 NEW LOCALITIES FOR RARE BUTTERFLIES MUSCHAMPIA CRIBRELLUM AND MELITAEA ORNATA (LEPIDOPTERA: HESPERIIDAE, NYMPHALIDAE) IN SERBIA Mihailo Vujić , Milan Djurić , ivan ToT HabiProt, Cankareva 9/13, 21000 Novi Sad, Serbia, e-mails: [email protected]; [email protected]; [email protected] Abstract – During project related field survey, performed from 9 th to 13 th of May, 2020, in southeastern Serbia, two rare butterfly species were registered at locality izvor, at rudina planina Mt. – Melitaea ornata and Muschampia cribrellum. New records of these species and confirmation of M. ornata at locality Šaprance suggest huge potential and invite further faunistic research in southeastern Serbia. Key worDS : Lepidoptera, Hesperiidae, Nymphalidae, fauna, Serbia Izvleček – NoVA NAjDiŠČA reDKiH MeTuLjeV MUSCHAMPIA CRIBREL - LUM iN MELITAEA ORNATA (LePiDoPTerA: HeSPeriiDAe, NyMPHALi - DAe) V SrBiji Med terenskimi raziskavami povezanimi s projektom, ki so potekale od 9. do 13. maja 2020 v jugovzhodni Srbiji, smo na najdišču izvor na rudini planini našli dve redki vrsti metuljev – Melitaea ornata in Muschampia cribrellum. Nove najdbe teh vrst in potrditev vrste M. ornata na najdišču Šaprance kažejo na velik potencial in vabijo k nadaljnim favnističnim raziskavam v jugovzhodni Srbiji. KLjuČNe BeSeDe : Lepidoptera, Hesperiidae, Nymphalidae, favna, Srbija At locality izvor, on rudina planina Mt. in southeastern Serbia (42.513227 N 22.519799 e, 954 m.a.s.l.), we recorded two rare butterfly species – Melitaea ornata Christoph, 1893 and Muschampia cribrellum (eversmann, 1841). Spinose skipper (Muschampia cribrellum) is a rare and local species from family Hesperiidae. its range includes large part of Asia and only small part of europe, and its presence was confirmed from romania, Bulgaria, North Macedonia and Serbia.
    [Show full text]
  • ARTIGO / ARTÍCULO / ARTICLE Melitaea Ornata (Cristoph, 1893), Nueva Especie Para La Península Ibérica
    ISSN: 1989-6581 Sánchez Mesa & Muñoz Sariot (2017) www.aegaweb.com/arquivos_entomoloxicos ARQUIVOS ENTOMOLÓXICOS, 18: 313-324 ARTIGO / ARTÍCULO / ARTICLE Melitaea ornata (Cristoph, 1893), nueva especie para la Península Ibérica. Primeros datos de su morfología, biología y ecología comparada con los de Melitaea phoebe (Denis & Schiffermüller, 1775). (Lepidoptera: Nymphalidae). Luis Sánchez Mesa 1 & Miguel Ginés Muñoz Sariot 2 1 e-mail: [email protected] 2 e-mail: [email protected] Resumen: Por primera vez se da a conocer la presencia de la especie Melitaea ornata (Cristoph, 1893) (Lepidoptera: Nymphalidae) en la Península Ibérica, en las provincias españolas de Granada, Jaén y Albacete, tras la colecta de una serie de orugas en último estadio (L7) que mostraban caracteres diferentes a los de Melitaea phoebe (Denis & Schiffermüller, 1775). Se describe e ilustra someramente la oruga adulta y es comparada con la de M. phoebe. Se describe e ilustra el imago de M. ornata, señalando como rasgo morfológico distintivo sus antenas. Se ilustra el huevo, pupa e imagos. Asimismo se aportan los primeros datos de su biología y ecología. Palabras clave: Lepidoptera, Nymphalidae, Melitaea, ornata, phoebe, estadios inmaduros, morfología, biología, ecología, distribución, Península Ibérica. Abstract: Melitaea ornata (Cristoph, 1893), new species for the Iberian Peninsula. First data of its morphology, biology and ecology compared with those of Melitaea phoebe (Denis & Schiffermüller, 1775). (Lepidoptera: Nymphalidae). The presence of the species Melitaea ornata (Cristoph, 1893) (Lepidoptera: Nymphalidae) is reported for the first time in the Iberian Peninsula, in the Spanish provinces of Granada, Jaén and Albacete, collecting a series of caterpillars in the last stage (L7) showing characters different to those of Melitaea phoebe (Denis & Schiffermüller, 1775).
    [Show full text]