plants Article Plant Disease Control Efficacy of Platycladus orientalis and Its Antifungal Compounds Sohyun Bae 1,2,†, Jae Woo Han 2,†, Quang Le Dang 3, Hun Kim 1,2,* and Gyung Ja Choi 1,2,* 1 Center for Eco-Friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, Korea;
[email protected] 2 Department of Medicinal Chemistry and Pharmacology, University of Science and Technology, Daejeon 34113, Korea;
[email protected] 3 Research and Development Center of Bioactive Compounds, Vietnam Institute of Industrial Chemistry, Hanoi 100000, Vietnam;
[email protected] * Correspondence:
[email protected] (H.K.);
[email protected] (G.J.C.) † These authors contributed equally to this work. Abstract: Plants contain a number of bioactive compounds that exhibit antimicrobial activity, which can be recognized as an important source of agrochemicals for plant disease control. In searching for natural alternatives to synthetic fungicides, we found that a methanol extract of the plant species Platycladus orientalis suppressed the disease development of rice blast caused by Magnaporthe oryzae. Through a series of chromatography procedures in combination with activity-guided fractionation, we isolated and identified a total of eleven compounds including four labdane-type diterpenes (1–4), six isopimarane-type diterpenes (5–10), and one sesquiterpene (11). Of the identified compounds, the MIC values of compounds 1, 2, 5 & 6 mixture, 9, and 11 ranged from 100 to 200 µg/mL against M. oryzae, whereas the other compounds were over 200 µg/mL. When rice plants were treated with the antifungal compounds, compounds 1, 2, and 9 effectively suppressed the development of rice Citation: Bae, S.; Han, J.W.; Dang, blast at all concentrations tested by over 75% compared to the non-treatment control.