Anti-RNF2 / RING1B / BAP1 Antibody (ARG55488)

Product datasheet [email protected]

ARG55488 Package: 100 μl anti-RNF2 / RING1B / BAP1 antibody Store at: -20°C

Summary

Product Description Rabbit Polyclonal antibody recognizes RNF2 / RING1B / BAP1

Tested Reactivity Hu, Ms, Rat

Tested Application ChIP, ICC/IF, IHC-P, IP, WB

Host Rabbit

Clonality Polyclonal

Isotype IgG

Target Name RNF2 / RING1B / BAP1

Antigen Species Human

Immunogen Recombinant protein of Human RNF2 (NP_009143.1)

Conjugation Un-conjugated

Alternate Names DING; RING1b; RING1B; EC 6.3.2.-; RING finger protein 1B; RING finger protein 2; RING2; RING finger protein BAP-1; BAP-1; HIPI3; BAP1; Protein DinG; E3 ubiquitin-protein ligase RING2; Huntingtin- interacting protein 2-interacting protein 3; HIP2-interacting protein 3

Application Instructions

Application table Application Dilution

ChIP 1:50 - 1:200

ICC/IF 1:50 - 1:200

IHC-P 1:50 - 1:200

IP 1:50 - 1:200

WB 1:500 - 1:2000

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control Jurkat

Calculated Mw 38 kDa

Observed Size ~ 38 kDa

Properties

Form Liquid

Purification Affinity purification with immunogen.

Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol

www.arigobio.com 1/3 Preservative 0.02% Sodium azide

Stabilizer 50% Glycerol

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation Gene Symbol RNF2 Gene Full Name ring finger protein 2 Background Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity. [provided by RefSeq, Jul 2008] Function E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation. H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes. [UniProt] Research Area Gene Regulation antibody; Polycomb Complexes antibody

Images

ARG55488 anti-RNF2 / RING1B / BAP1 antibody ICC/IF image

Immunofluorescence: U20S cells stained with ARG55488 anti-RNF2 / RING1B / BAP1 antibody.

www.arigobio.com 2/3 ARG55488 anti-RNF2 / RING1B / BAP1 antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue stained with ARG55488 anti-RNF2 / RING1B / BAP1 antibody at 1:100 dilution.

ARG55488 anti-RNF2 / RING1B / BAP1 antibody WB image

Western blot: Jurkat cell lysate stained with ARG55488 anti-RNF2 / RING1B / BAP1 antibody.

ARG55488 anti-RNF2 / RING1B / BAP1 antibody IP image

Immunoprecipitation: 200 µg extracts of 293T cells were immunoprecipitated and stained with ARG55488 anti-RNF2 / RING1B / BAP1 antibody at 1:1000 dilution.