DOCSLIB.ORG

PROTEIN DISULFIDE ISOMERASE LIKE 5-1 Is a Susceptibility Factor to Plant Viruses

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
PROTEIN DISULFIDE ISOMERASE LIKE 5-1 Is a Susceptibility Factor to Plant Viruses PROTEIN DISULFIDE ISOMERASE LIKE 5-1 is a susceptibility factor to plant viruses Ping Yanga, Thomas Lüpkenb, Antje Habekussb, Goetz Henselc, Burkhard Steuernageld,1, Benjamin Kiliana, Ruvini Ariyadasaa, Axel Himmelbacha, Jochen Kumlehnc, Uwe Scholzd, Frank Ordonb,2, and Nils Steina,2,3 aGenome Diversity, Department Genebank, Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany; bInstitute for Resistance Research and Stress Tolerance, Julius Kuehn Institute, Federal Research Centre for Cultivated Plants, D-06484 Quedlinburg, Germany; cPlant Reproductive Biology, Department of Physiology and Cell Biology, Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany; and dBioinformatics and Information Technology, Department of Cytogenetics and Genome Analyses, Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany Edited by David C. Baulcombe, University of Cambridge, Cambridge, United Kingdom, and approved January 3, 2014 (received for review October 29, 2013) Protein disulfide isomerases (PDIs) catalyze the correct folding of (10, 11). In addition, to establish replication and assembly com- proteins and prevent the aggregation of unfolded or partially plexes during infection, viruses typically create membrane-bound folded precursors. Whereas suppression of members of the PDI environments, referred to as “virus factories” (12). There, cellular gene family can delay replication of several human and animal chaperones such as HSP70 and DNAJ-like proteins likely contrib- viruses (e.g., HIV), their role in interactions with plant viruses is ute to the correct folding and translocation of substrates (12, 13). largely unknown. Here, using a positional cloning strategy we However, only a few such host factors are known (7, 9, 14). identified variants of PROTEIN DISULFIDE ISOMERASE LIKE 5–1 Barley yellow mosaic virus disease caused by barley yellow (HvPDIL5-1) as the cause of naturally occurring resistance to mul- mosaic virus (BaYMV) and barley mild mosaic virus (BaMMV) tiple strains of Bymoviruses. The role of wild-type HvPDIL5-1 in (both belong to Bymoviruses) seriously threatens winter barley conferring susceptibility was confirmed by targeting induced local production in Europe and East Asia (4). Infection leads to yel- lesions in genomes for induced mutant alleles, transgene-induced low discoloration and stunted growth and may result in yield loss complementation, and allelism tests using different natural resis- of up to 50% (15). Soil-borne transmission via the plasmodio- tance alleles. The geographical distribution of natural genetic var- phorid Polymyxa graminis prohibits plant protection by chemical iants of HvPDIL5-1 revealed the origin of resistance conferring measures, and breeding for resistant cultivars is therefore the alleles in domesticated barley in Eastern Asia. Higher sequence only practicable way to prevent yield loss. The naturally occur- ring recessive resistance locus rym11 confers complete broad- diversity was correlated with areas with increased pathogen di- spectrum resistance to all known European isolates of BaMMV versity suggesting adaptive selection for bymovirus resistance. and BaYMV (16–19). In the present study, we used a positional HvPDIL5-1 homologs are highly conserved across species of the cloning strategy to identify the gene underlying rym11-based re- plant and animal kingdoms implying that orthologs of HvPDIL5-1 PDI sistance. We show that it is a susceptibility factor belonging to or other closely related members of the gene family may be a gene family of PROTEIN DISULFIDE ISOMERASES (PDIs), potential susceptibility factors to viruses in other eukaryotic species. and is highly conserved across eukaryotic species. We observe a strong correlation between natural allelic variation and geographic allele mining | resistance breeding | soil-borne virus disease | chaperone distribution, suggesting that both the origin and subsequent nfectious diseases caused by plant viruses threaten agricultural Significance Iproductivity and reduce globally attainable agricultural pro- duction by about 3% (1). In specific pathosystems, plant viruses This work describes a susceptibility factor to plant viruses that can result in the loss of the entire crop. For example, the dev- belongs to the conserved PROTEIN DISULFIDE ISOMERASE (PDI) astation of cassava production by cassava mosaic geminiviruses gene family. We show that loss-of-function HvPDIL5-1 alleles (CMGs) in Uganda during the 1990s led to widespread food at the recessive RESISTANCE TO YELLOW MOSAIC DISEASE 11 shortages and famine-related deaths (2). Unfortunately pro- (rym11) resistance locus confer broad-spectrum resistance to tecting plants against viruses (especially soil-borne viruses) by multiple strains of Bymoviruses and could therefore play a using agrochemicals to control virus vectors is seldom efficient central role in durable virus resistance breeding in barley. The from economic or ecological perspectives. Therefore, crop pro- geographic distribution of functional alleles of rym11 in East tection based on naturally occurring virus resistance is key to Asia suggests adaptive selection for resistance in this region. minimizing losses and achieving sustainable crop yields. Orthologues of HvPDIL5-1 or related members of the PDI gene Positive-strand RNA viruses represent the largest group of family potentially provide susceptibility factors to viruses across plant viruses (3). They cause a very high proportion of the im- portant infectious virus diseases in agriculture (4, 5). Such plant animal and plant kingdoms. viruses carry a reduced genome that encodes a limited set of – — Author contributions: F.O. and N.S. designed research; T.L. mapped and identified the can- functional proteins (4 10 viral proteins) insufficient to com- didate gene HvPDIL5-1; P.Y., A. Habekuss, G.H., A. Himmelbach, and J.K. performed research; plete the entire virus replication and proliferation cycle (6). In- P.Y., T.L., B.S., B.K., R.A., and U.S. analyzed data; and P.Y. and N.S. wrote the paper. stead, over evolutionary time, viruses recruited host factors to Conflict of interest statement: A patent application has been filed relating to this work. perform the infectious life cycle (7). This dependence on host factors establishes a possibility that plants can evolve escape, tol- This article is a PNAS Direct Submission. erance, or resistance mechanisms to ameliorate the consequences Freely available online through the PNAS open access option. of viral infection. The absence of essential host factors could in- Data deposition: The sequences reported in this paper have been deposited in the EMBL terfere with the infection process or restrict proliferation (8) lead- database (accession nos. PRJEB4765 and HG793095). ing to either mono- or polygenic recessive resistance (5). Prominent 1Present address: The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, examples of such susceptibility factors that are conserved in mul- United Kingdom. tiple plant–virus systems are the EUKARYOTIC TRANSLATION 2F.O. and N.S. contributed equally to this work. INITIATION FACTORS (EIF)4E, iso4E, 4G, and iso4G (9). 3To whom correspondence should be addressed. E-mail: [email protected]. Translation initiation factors may interact directly with viral RNA This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10. where they catalyze the initiation of translation of viral polyproteins 1073/pnas.1320362111/-/DCSupplemental. 2104–2109 | PNAS | February 11, 2014 | vol. 111 | no. 6 www.pnas.org/cgi/doi/10.1073/pnas.1320362111 Downloaded by guest on September 24, 2021 was therefore a likely candidate for rym11-based resistance. The allele carrying the 1,375-bp deletion was named rym11-a. Functional Validation of HvPDIL5-1 as a Susceptibility Factor for Bymovirus Infection. We used three independent approaches to validate the hypothesis that HvPDIL5-1 is a susceptibility factor for bymovirus infection. First, we screened an EMS-induced mutant population of barley by targeting induced local lesions in genomes (TILLING) (21). Two independent mutants 9699 and 10253 were identified each carrying a premature STOP codon in the coding sequence (CDS) of HvPDIL5-1 (SI Appendix, Table S1 and Fig. 1B). M3 progeny of the originally heterozygous M2 plants segregated for virus resistance. After phenotyping, the plants were genotyped for the presence of the mutation. All homozygous mutant M3 plants were found to be completely re- sistant. Homozygous wild-type and heterozygous M3 plants were predominantly susceptible with infection rates comparable to the susceptible controls (Table 1 and SI Appendix, Table S2). Re- sistance tests were replicated twice in M4 progeny of a homozy- gous mutant and a wild-type M3 plant of each of the mutant lines 9699 and 10253, respectively. All homozygous mutant M4 plants were tested resistant. Homozygous wild-type M4 progeny were susceptible, with infection rates between 66% and 100%, similar to susceptible controls (Table 1 and SI Appendix, Table S2 and Fig. S3). Mechanically infected homozygous wild-type HvPDIL5- 1 M4 progenies showed the characteristic yellow discoloration during vegetative growth stage (Fig. 1C), stunted growth at heading stage (Fig. 1D), as well as late maturation (Fig. 1E). Mock in- oculated homozygous mutant M4 individuals did not show any detectable difference in overall growth habit at heading and Fig. 1. Map-based
Load more

Recommended publications
  • ICTV Code Assigned: 2011.001Ag Officers)
    This form should be used for all taxonomic proposals. Please complete all those modules that are applicable (and then delete the unwanted sections). For guidance, see the notes written in blue and the separate document “Help with completing a taxonomic proposal” Please try to keep related proposals within a single document; you can copy the modules to create more than one genus within a new family, for example. MODULE 1: TITLE, AUTHORS, etc (to be completed by ICTV Code assigned: 2011.001aG officers) Short title: Change existing virus species names to non-Latinized binomials (e.g. 6 new species in the genus Zetavirus) Modules attached 1 2 3 4 5 (modules 1 and 9 are required) 6 7 8 9 Author(s) with e-mail address(es) of the proposer: Van Regenmortel Marc, [email protected] Burke Donald, [email protected] Calisher Charles, [email protected] Dietzgen Ralf, [email protected] Fauquet Claude, [email protected] Ghabrial Said, [email protected] Jahrling Peter, [email protected] Johnson Karl, [email protected] Holbrook Michael, [email protected] Horzinek Marian, [email protected] Keil Guenther, [email protected] Kuhn Jens, [email protected] Mahy Brian, [email protected] Martelli Giovanni, [email protected] Pringle Craig, [email protected] Rybicki Ed, [email protected] Skern Tim, [email protected] Tesh Robert, [email protected] Wahl-Jensen Victoria, [email protected] Walker Peter, [email protected] Weaver Scott, [email protected] List the ICTV study group(s) that have seen this proposal: A list of study groups and contacts is provided at http://www.ictvonline.org/subcommittees.asp .
    [Show full text]
  • Evidence to Support Safe Return to Clinical Practice by Oral Health Professionals in Canada During the COVID-19 Pandemic: a Repo
    Evidence to support safe return to clinical practice by oral health professionals in Canada during the COVID-19 pandemic: A report prepared for the Office of the Chief Dental Officer of Canada. November 2020 update This evidence synthesis was prepared for the Office of the Chief Dental Officer, based on a comprehensive review under contract by the following: Paul Allison, Faculty of Dentistry, McGill University Raphael Freitas de Souza, Faculty of Dentistry, McGill University Lilian Aboud, Faculty of Dentistry, McGill University Martin Morris, Library, McGill University November 30th, 2020 1 Contents Page Introduction 3 Project goal and specific objectives 3 Methods used to identify and include relevant literature 4 Report structure 5 Summary of update report 5 Report results a) Which patients are at greater risk of the consequences of COVID-19 and so 7 consideration should be given to delaying elective in-person oral health care? b) What are the signs and symptoms of COVID-19 that oral health professionals 9 should screen for prior to providing in-person health care? c) What evidence exists to support patient scheduling, waiting and other non- treatment management measures for in-person oral health care? 10 d) What evidence exists to support the use of various forms of personal protective equipment (PPE) while providing in-person oral health care? 13 e) What evidence exists to support the decontamination and re-use of PPE? 15 f) What evidence exists concerning the provision of aerosol-generating 16 procedures (AGP) as part of in-person
    [Show full text]
  • Molecular Analysis of Vanilla Mosaic Virus from the Cook Islands
    Molecular Analysis of Vanilla mosaic virus from the Cook Islands Christopher Puli’uvea A thesis submitted to Auckland University of Technology in partial fulfilment of the requirements for the degree of Master of Science (MSc) 2017 School of Science I Abstract Vanilla was first introduced to French Polynesia in 1848 and from 1899-1966 was a major export for French Polynesia who then produced an average of 158 tonnes of cured Vanilla tahitensis beans annually. In 1967, vanilla production declined rapidly to a low of 0.6 tonnes by 1981, which prompted a nation-wide investigation with the aim of restoring vanilla production to its former levels. As a result, a mosaic-inducing virus was discovered infecting V. tahitensis that was distinct from Cymbidium mosaic virus (CyMV) and Odontoglossum ringspot virus (ORSV) but serologically related to dasheen mosaic virus (DsMV). The potyvirus was subsequently named vanilla mosaic virus (VanMV) and was later reported to infect V. tahitensis in the Cook Islands and V. planifolia in Fiji and Vanuatu. Attempts were made to mechanically inoculate VanMV to a number of plants that are susceptible to DsMV, but with no success. Based on a partial sequence analysis, VanMV-FP (French Polynesian isolate) and VanMV-CI (Cook Islands isolate) were later characterised as strains of DsMV exclusively infecting vanilla. Since its discovery, little information is known about how VanMV-CI acquired the ability to exclusively infect vanilla and lose its ability to infect natural hosts of DsMV or vice versa. The aims of this research were to characterise the VanMV genome and attempt to determine the molecular basis for host range specificity of VanMV-CI.
    [Show full text]
  • Progress and Prospects of Studies on Polymyxa Graminis and Its Transmitted Cereal Viruses in China＊
    PROGRESS IN NATURAL SCIENCE V ol .15 , No .6 , June 2005 REVIEW ARTICLE Progress and prospects of studies on Polymyxa graminis and its transmitted cereal viruses in China＊ CH EN Jianping ＊＊ (Virology and Biotechnology Institute , Zhejiang Academy of Agricultural Sciences, Key Laboratory of Plant Virology , Ministry of Agri- culture and Zhejiang Province, Hangzhou 310021, China) Received October 8 , 2004 ;revised October 25 , 2004 Abstract Polymyxa gram inis is a eukaryotic obligate biotrophic parasite of plant roots that belongs to a poorly studied discrete taxonomic unit informally called “ plasmodiophorids” .P .graminis is nonpathogenic , but has the ability to acquire and transmit nine plant viruses w hich belong to genera Bymovirus and Furovirus and cause serious diseases in cereal crop speciesand also result in significant yield reductions in China and elsew here.Genus Bymovirus contains barley yellow mosaic virus (BaYMV), barley mild mosaic virus (BaMMV), w heat yellow mosaic virus (WYMV), w heat spindle streak mosaic virus (WSS MV), and oat mosaic virus (OMV), and genus F urovirus contains soil-borne w heat mosaic virus(S BW MV), oat golden stripe virus(OGSV), and new ly identified Chinese w heat mosaic virus(CWM V)and soil-borne cereal mosaic virus(S BCMV).All these viruses have been sequenced and their w orldw ide distribu- tions have been studied .The viruses are protected by the environment w ithin P .gra minis resting spores that may remain dormant but vi- able for decades(probably until a suitable host plant is encountered).S pontaneous deletion mutants of S BW MV , OGS V and OMV are de- tected , and these deletion mutants are not transmissible by the fungus.The persistent, soil-borne nature of these diseases makes the use of virus-resistant crop varieties cu rren tly the only practical and environmentally friendly means to control them , and a large number of disease resistant germ plasms have been screened .
    [Show full text]
  • Multipartite Viruses: Organization, Emergence and Evolution
    UNIVERSIDAD AUTÓNOMA DE MADRID FACULTAD DE CIENCIAS DEPARTAMENTO DE BIOLOGÍA MOLECULAR MULTIPARTITE VIRUSES: ORGANIZATION, EMERGENCE AND EVOLUTION TESIS DOCTORAL Adriana Lucía Sanz García Madrid, 2019 MULTIPARTITE VIRUSES Organization, emergence and evolution TESIS DOCTORAL Memoria presentada por Adriana Luc´ıa Sanz Garc´ıa Licenciada en Bioqu´ımica por la Universidad Autonoma´ de Madrid Supervisada por Dra. Susanna Manrubia Cuevas Centro Nacional de Biotecnolog´ıa (CSIC) Memoria presentada para optar al grado de Doctor en Biociencias Moleculares Facultad de Ciencias Departamento de Biolog´ıa Molecular Universidad Autonoma´ de Madrid Madrid, 2019 Tesis doctoral Multipartite viruses: Organization, emergence and evolution, 2019, Madrid, Espana. Memoria presentada por Adriana Luc´ıa-Sanz, licenciada en Bioqumica´ y con un master´ en Biof´ısica en la Universidad Autonoma´ de Madrid para optar al grado de doctor en Biociencias Moleculares del departamento de Biolog´ıa Molecular en la facultad de Ciencias de la Universidad Autonoma´ de Madrid Supervisora de tesis: Dr. Susanna Manrubia Cuevas. Investigadora Cient´ıfica en el Centro Nacional de Biotecnolog´ıa (CSIC), C/ Darwin 3, 28049 Madrid, Espana. to the reader CONTENTS Acknowledgments xi Resumen xiii Abstract xv Introduction xvii I.1 What is a virus? xvii I.2 What is a multipartite virus? xix I.3 The multipartite lifecycle xx I.4 Overview of this thesis xxv PART I OBJECTIVES PART II METHODOLOGY 0.5 Database management for constructing the multipartite and segmented datasets 3 0.6 Analytical
    [Show full text]
  • Serological and Genomic Characterization of Two Plant RNA Viruses: Barley Mild Mosaic Virus (Bammv) and a Potential Cryptic Virus from Pine (Pinus Sylvestris)
    Serological and genomic characterization of two plant RNA viruses: Barley Mild Mosaic Virus (BaMMV) and a potential cryptic virus from pine (Pinus sylvestris) Ph.D. thesis by Dorina Veliceasa Supervisor: Dr. Noémi Lukács Institute of Plant Biology, Biological Research Centre Hungarian Academy of Sciences Szeged, 2005 TABLE OF CONTENTS Acknowledgements i Abbreviations ii General Introduction iii 1. RESEARCH BACKGROUNG 1 1.1 Double-stranded RNA viruses 1 1.1.1 Plant cryptic viruses 1 1.1.2 dsRNA virus RNA-dependent RNA polymerase 5 1.1.3 dsRNA viruses: origin and phylogenetic implications 11 1.2 Single-stranded, messengers sense RNA viruses 13 1.2.1 Barley mild mosaic virus (BaMMV) 13 1.2.2 Serological relationships between different BaMMV isolates and BaYMV 17 1.2.3 Epitope mapping by using multipin overlapping peptide fragments 20 2. OBJECTIVES 24 3. MATERIAL AND METHODS 26 3.1 Reagents 26 3.2 Plant material and virus isolates 26 3.2.1 Plant material 26 3.2.2 Virus isolates 26 3.3 dsRNA isolation 26 3.4 cDNA synthesis 27 3.5. Cloning 27 3.6. Sequence analysis 28 3.7. Nucleotide sequence data 28 3.8. Preparation of the antigen 29 3.9. Production of monoclonal antibodies (MAb) 29 3.10. Synthesis of peptides 29 3.11. Assay of monoclonal antibodies 30 3.11.1. Enzyme-linked immunosorbent assay (ELISA) of hybrid cell lines 30 3.11.2. Triple antibody sandwich (TAS) ELISA 30 3.11.3. ELISA on peptides 31 3.11.4. Dot blot 32 3.11.5. Western blot (WB) analysis 32 3.11.6.
    [Show full text]
  • A Member of a New Genus in the Potyviridae Infects Rubus James Susaimuthu A,1, Ioannis E
    Available online at www.sciencedirect.com Virus Research 131 (2008) 145–151 A member of a new genus in the Potyviridae infects Rubus James Susaimuthu a,1, Ioannis E. Tzanetakis b,∗,1, Rose C. Gergerich a, Robert R. Martin b,c a Department of Plant Pathology, University of Arkansas, Fayetteville 72701, United States b Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331, United States c Horticultural Crops Research Laboratory, USDA-ARS, Corvallis 97330, United States Received 6 March 2007; received in revised form 30 August 2007; accepted 1 September 2007 Available online 22 October 2007 Abstract Blackberry yellow vein disease causes devastating losses on blackberry in the south and southeastern United States. Blackberry yellow vein associated virus (BYVaV) was identified as the putative causal agent of the disease but the identification of latent infections of BYVaV led to the investigation of additional agents being involved in symptomatology. A potyvirus, designated as Blackberry virus Y (BVY), has been identified in plants with blackberry yellow vein disease symptoms also infected with BYVaV. BVY is the largest potyvirus sequenced to date and the first to encode an AlkB domain. The virus shows minimal sequence similarity with known members of the family and should be con- sidered member of a novel genus in the Potyviridae. The relationship of BVY with Bramble yellow mosaic virus, the only other potyvirus known to infect Rubus was investigated. The presence of the BVY was verified in several blackberry plants, but it is not the causal agent of blackberry yellow vein disease since several symptomatic plants were not infected with the virus and BVY was also detected in asymptomatic plants.
    [Show full text]
  • Vector-Borne Viruses of Pulse Crops, with a Particular Emphasis on North American Cropping System
    Annals of the Entomological Society of America, 111(4), 2018, 205–227 doi: 10.1093/aesa/say014 Special Collection: Pulse Crop Insect Pests and Their Review Management Strategies Vector-Borne Viruses of Pulse Crops, With a Particular Emphasis on North American Cropping System Arash Rashed,1,6 Xue Feng,2 Sean M. Prager,3 Lyndon D. Porter,4 Janet J. Knodel,5 Alexander Karasev,2 and Sanford D. Eigenbrode2 1Department of Entomology, Plant Pathology and Nematology, Aberdeen Research and Extension Center, University of Idaho, Aberdeen, ID 83210, 2Department of Entomology, Plant Pathology and Nematology, University of Idaho, Moscow, ID 83844, 3Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada, 4Grain Legume Genetics and Physiology Research Unit, Agricultural Research Service, United States Department of Agriculture, Prosser, WA 99350, 5Department of Plant Pathology, North Dakota State University, Fargo, ND 58108, and 6Corresponding author, e-mail: [email protected] Subject Editor: Gadi V. P. Reddy Received 23 February 2018; Editorial decision 2 April 2018 Abstract Due to their nutritional value and function as soil nitrogen fixers, production of pulses has been increasing markedly in the United States, notably in the dryland areas of the Northern Plains and the Pacific Northwest United States (NP&PNW). There are several insect-transmitted viruses that are prevalent and periodically injuri- ous to pulse crops in the NP&PNW and elsewhere in North America. Others are currently of minor concern, occurring over limited areas or sporadically. Others are serious constraints for pulses elsewhere in the world and are not currently known in North America, but have the potential to be introduced with significant economic consequences.
    [Show full text]
  • Deep Roots and Splendid Boughs of the Global Plant Virome
    PY58CH11_Dolja ARjats.cls May 19, 2020 7:55 Annual Review of Phytopathology Deep Roots and Splendid Boughs of the Global Plant Virome Valerian V. Dolja,1 Mart Krupovic,2 and Eugene V. Koonin3 1Department of Botany and Plant Pathology and Center for Genome Research and Biocomputing, Oregon State University, Corvallis, Oregon 97331-2902, USA; email: [email protected] 2Archaeal Virology Unit, Department of Microbiology, Institut Pasteur, 75015 Paris, France 3National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA Annu. Rev. Phytopathol. 2020. 58:11.1–11.31 Keywords The Annual Review of Phytopathology is online at plant virus, virus evolution, virus taxonomy, phylogeny, virome phyto.annualreviews.org https://doi.org/10.1146/annurev-phyto-030320- Abstract 041346 Land plants host a vast and diverse virome that is dominated by RNA viruses, Copyright © 2020 by Annual Reviews. with major additional contributions from reverse-transcribing and single- All rights reserved stranded (ss) DNA viruses. Here, we introduce the recently adopted com- prehensive taxonomy of viruses based on phylogenomic analyses, as applied to the plant virome. We further trace the evolutionary ancestry of distinct plant virus lineages to primordial genetic mobile elements. We discuss the growing evidence of the pivotal role of horizontal virus transfer from in- vertebrates to plants during the terrestrialization of these organisms, which was enabled by the evolution of close ecological associations between these diverse organisms. It is our hope that the emerging big picture of the forma- tion and global architecture of the plant virome will be of broad interest to plant biologists and virologists alike and will stimulate ever deeper inquiry into the fascinating field of virus–plant coevolution.
    [Show full text]
  • Full Genome Evolutionary Studies of Wheat Streak Mosaic-Associated Viruses Using High-Throughput
    fmicb-12-699078 July 26, 2021 Time: 18:4 # 1 ORIGINAL RESEARCH published: 30 July 2021 doi: 10.3389/fmicb.2021.699078 Full Genome Evolutionary Studies of Wheat Streak Mosaic-Associated Viruses Using High-Throughput Edited by: Sequencing Akhtar Ali, University of Tulsa, United States Carla Dizon Redila, Savannah Phipps† and Shahideh Nouri* Reviewed by: Department of Plant Pathology, College of Agriculture, Kansas State University, Manhattan, KS, United States Xifeng Wang, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Wheat streak mosaic (WSM), a viral disease affecting cereals and grasses, causes Institute of Plant Protection (CAAS), China substantial losses in crop yields. Wheat streak mosaic virus (WSMV) is the main causal Artemis Rumbou, agent of the complex, but mixed infections with Triticum mosaic virus (TriMV) and High Humboldt University of Berlin, plains wheat mosaic emaravirus (HPWMoV) were reported as well. Although resistant Germany Satyanarayana Tatineni, varieties are effective for the disease control, a WSMV resistance-breaking isolate Agricultural Research Service, and several potential resistance-breaking isolates have been reported, suggesting that United States Department of Agriculture, United States viral populations are genetically diverse. Previous phylogenetic studies of WSMV were *Correspondence: conducted by focusing only on the virus coat protein (CP) sequence, while there is no Shahideh Nouri such study for either TriMV or HPWMoV. Here, we studied the genetic variation and [email protected] evolutionary mechanisms of natural populations of WSM-associated viruses mainly in †Present address: Savannah Phipps, Kansas fields and fields in some other parts of the Great Plains using high-throughput Department of Crop and Soil RNA sequencing.
    [Show full text]
  • Principles and Applications in Plant Virology
    plants Review Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology 1, , 2, 3, 1, Stefano Panno * y, Slavica Mati´c y, Antonio Tiberini y, Andrea Giovanni Caruso y , Patrizia Bella 1, Livio Torta 1 , Raffaele Stassi 1 and Salvatore Davino 1,4,* 1 Department of Agricultural, Food and Forest Sciences, University of Palermo, 90128 Palermo, Italy; [email protected] (A.G.C.); [email protected] (P.B.); [email protected] (L.T.); [email protected] (R.S.) 2 Department of Agricultural, Forestry and Food Sciences, University of Turin, 10095 Turin, Italy; [email protected] 3 Council for Agricultural Research and Economics, Research Center for Plant Protection and Certification, 00156 Rome, Italy; [email protected] 4 Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), 10135 Turin, Italy * Correspondence: [email protected] (S.P.); [email protected] (S.D.); Tel.: +39-0912-389-6049 (S.P. & S.D.) These authors contributed equally to this work. y Received: 6 March 2020; Accepted: 2 April 2020; Published: 6 April 2020 Abstract: In the last decades, the evolution of molecular diagnosis methods has generated different advanced tools, like loop-mediated isothermal amplification (LAMP). Currently, it is a well-established technique, applied in different fields, such as the medicine, agriculture, and food industries, owing to its simplicity, specificity, rapidity, and low-cost efforts. LAMP is a nucleic acid amplification under isothermal conditions, which is highly compatible with point-of-care (POC) analysis and has the potential to improve the diagnosis in plant protection. The great advantages of LAMP have led to several upgrades in order to implement the technique.
    [Show full text]
  • ICTV Virus Taxonomy Profile: Potyviridae
    ICTV VIRUS TAXONOMY PROFILES Wylie et al., Journal of General Virology 2017;98:352–354 DOI 10.1099/jgv.0.000740 ICTV ICTV Virus Taxonomy Profile: Potyviridae Stephen J. Wylie,1,* Mike Adams,2 Celia Chalam,3 Jan Kreuze,4 Juan Jose López-Moya,5 Kazusato Ohshima,6 Shelly Praveen,7 Frank Rabenstein,8 Drake Stenger,9 Aiming Wang,10 F. Murilo Zerbini11 and ICTV Report Consortium Abstract The Potyviridae is the largest family of RNA plant viruses, members of which have single-stranded, positive-sense RNA genomes and flexuous filamentous particles 680–900 nm long and 11–20 nm wide. There are eight genera, distinguished by the host range, genomic features and phylogeny of the member viruses. Genomes range from 8.2 to 11.3 kb, with an average size of 9.7 kb. Most genomes are monopartite but those of members of the genus Bymovirus are bipartite. Some members cause serious disease epidemics in cultivated plants. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Potyviridae, which is available at www.ictv.global/report/ potyviridae. Table 1. Characteristics of the family Potyviridae Typical member: potato virus Y-O (U09509), species Potato virus Y, genus Potyvirus Virion Non-enveloped, flexuous and filamentous capsid, 680–900 nm long and 11–20 nm in diameter with a single core capsid protein Genome 8–11 kb of positive-sense, single-stranded, usually monopartite RNA (bipartite in the genus Bymovirus) Replication Cytoplasmic, initiated in virus replication complexes on membranous vesicles at ER exit sites.
    [Show full text]