High Throughput Blood Processing Samples Using the New General Purpose Centrifuge

Application Note:

ANCFGGP3BBVACUT-0509 High Throughput Blood Processing Samples Using the New Thermo Scientiﬁc General Purpose Centrifuge

Nadia Boujtita, PhD, Thermo Fisher Scientiﬁc, Saint-Herblain, France

Introduction KEY WORDS Isolation of plasma from • High Throughput whole blood is a first step Processing for various in-vitro molecular diagnostic assays. A cur- • Plasma rent technique for plasma Preparation separation is to collect • Molecular whole blood into blood col- Diagnostic Test lection tubes, such as the Methods BD Vacutainer® tube con- taining anticoagulants • BIOLiner Swinging (EthyleneDiamineTetraacetic Bucket Rotor Acid (EDTA) or anticoagu- Thermo Scientific BIOLiner Swinging Bucket Rotor • General Purpose lant Citrate-Dextrose Centrifuge (ACD) solution) then to separate it from the other 2. After mixing, store the BD tube • BD Vacutainer blood components by cen- upright at room temperature until Tube trifugation using a swinging centrifugation. bucket rotor. Note: Blood samples should be cen- The obtained plasma can trifuged within 2 hours of blood col- be used in molecular diag- lection for best results. With nostic test methods, such as additional delay before centrifuga- polymerase chain reaction tion, red blood cell contamination of (PCR) or branched DNA the separated undiluted plasma sam- amplification techniques ple increases2. (bDNA), and for a wide 3. Centrifuge blood sample at room range of research and clini- temperature (18-25 °C) at 1,100 cal diagnostic applications Thermo Scientific general purpose centrifuge with BIOLiner x g for at least 10 min. where quality and integrity adapter for vacutainer tubes 4. Open the tube immediately after of undiluted plasma sample centrifugation; carefully pipette the is required. plasma into a separate container. The procedure can be conducted trifuge and the BIOLiner high capacity When using the BD Vacutainer PPT using the new Thermo Scientific gen- swinging bucket rotor. It also describes Plasma Preparation Tube most of eral purpose centrifuge series and the how to shorten processing time by in- the erythrocytes and granulocytes Thermo Scientific BIOLiner high ca- creasing throughput during the prepa- and some of the lymphocytes and pacity swinging bucket rotor. By using ration of plasma with BD Vacutainer monocytes are separated away from this equipment, productivity is in- PPT™ Plasma Preparation Tube. the supernatant. creased by preparing an undiluted Recommendations : plasma sample and throughput is sig- Procedure • Do not speed the BD tubes over nificantly maximized by spinning 196 The procedure described below out- 10,000 x g as this may cause tube 5/7 mL blood tubes per run versus 96 lines the process for preparation of breakage and exposure to blood to 120 tubes with competitive cen- undiluted plasma1 using the new gen- and possible injury. trifuges (see table 1) and 148 10 mL eral pupose centrifuge and the BIO- • Use special designed sealed contain- blood tubes/run versus 64 to 120 with Liner swinging bucket rotor with the ers during centrifugation in order to competitor models (see table 2). multiple carrier for 5/7 mL or 10 mL avoid any exposure to biologic This application brief describes a sealed tubes. samples (viral hepatitis, HIV procedure for preparation of plasma 1. After collecting whole blood in the (AIDS), or other infectious diseases) from whole blood using the new BD PPT tube, gently invert the BD or releasing of potentially haz- Thermo Scientific general purpose cen- tube 8 - 10 times. ardous materials. Results Table 1: Time savings in hours and percentages when using four different centrifuges for plasma In addition to these offices, The new Thermo Scientific general preparation into 5/7 mL blood tubes Thermo Fisher Scientific pupose centrifuge combined with maintains a network of CENTRIFUGES CAPACITY THROUGHPUT TIME NEEDED TO TIME SAVINGS EXPERIENCED representative organizations with the BIOLiner swinging bucket 5/7 ML TUBES TUBES CENTRIFUGE (h AND %) WITH THE rotor and BD Vacutainer tubes leads CENTRIFUGED/DAY 8000 TUBES THERMO SCIENTIFIC throughout the world. (40 SPINS)* GENERAL PURPOSE to reliable results and high quality CENTRIFUGE undiluted plasma samples, enabling Thermo Scientific 196 7840 8h // molecular diagnostic test and down- General Purpose stream clinical diagnostic applications. Centrifuge Competitor B 120 4800 13h 5h 38.5% Conclusion Centrifuge B 96 3840 17h 9h 53% The new general purpose centrifuge Competitor C 100 4000 16h 8h 50% North America: Centrifuge C USA / Canada enables extremely fast processing of +1 866 984 0039 Competitor D 96 3840 17h 9h 53% blood samples at the unique high ca- Europe: Centrifuge D pacity processing of the swinging Austria +43 1 801 40 0 bucket rotor with the multiple car- *Based on supposition that 5 spins can be performed/hour and that 10 min are needed to load/unload tubes/hour, i.e. with the Thermo Scientific general purpose centrifuge, this allowed us to perform 40 spins a day (~8h). Belgium rier for 5/7 mL sealed (196 x 5/7 mL Note: Blood samples (5/7 mL tubes) were centrifuged at room temperature (18-25 °C) at 1,100 x g for at least 10 min. +32 2 482 30 30 blood tubes/run) or for 10 mL sealed France tubes (148 x 10 mL blood +33 2 2803 2000 tubes/run). The swinging bucket Germany national toll free 08001-536 376 rotor includes a biocontainment lid Germany international to protect from aerosolization and +49 6184 90 6940 spills during centrifugation. Italy With this unique high-productivity Table 2: Time savings in hours and percentages when using four different centrifuges for plasma +39 02 02 95059 341 preparation into 10 mL blood tubes solution, achieve time savings of up to Netherlands +31 76 571 4440 56% (See Table 2) and up to 53% CENTRIFUGES CAPACITY THROUGHPUT TIME NEEDED TO TIME SAVINGS EXPERIENCED Nordic countries (See Table 1) when spinning 10 mL 5/7 ML TUBES TUBES CENTRIFUGE (h AND %) WITH THE +358 9 329 100 and 5/7 mL blood tubes respectively CENTRIFUGED/DAY 8000 TUBES THERMO SCIENTIFIC (40 SPINS)* GENERAL PURPOSE Russia / CIS and the environment is protected from CENTRIFUGE +7 (812) 703 42 15 Spain / Portugal biocontamination. Thermo Scientific 148 5920 11h // +34 93 223 09 18 Using this ideal new Thermo Sci- General Purpose Centrifuge Switzerland entific general purpose centrifuge, +41 44 454 12 12 with the BIOLiner swinging bucket Competitor B 96 3840 17h 6h 35.3% Centrifuge B UK / Ireland rotor and BD vacutainer tubes, en- +44 870 609 9203 Competitor C 64 2560 25h 14h 56% sures a high quality molecular test Asia: Centrifuge C China with the ability to increase through- Competitor D 96 3840 17h 9h 53% +86 21 6865 4588 or put by increasing blood sample pro- Centrifuge D +86 10 8419 3588 cessing capacity. India *Based on supposition that 5 spins can be performed/hour and that 10 min are needed to load/unload tubes/hour, i.e. with +91 22 6716 2200 the Thermo Scientific general purpose centrifuge, this allowed us to perform 40 spins a day (~8h). References Japan Note: Blood samples (10 mL tubes) were centrifuged at room temperature (18-25 °C) at 1,100 x g for at least 10 min. +81 45 453 9220 1. Procedure according to BD proto- Other Asian countries col of the Kit or Assay Manufac- +852 2885 4613 ture’s Instructions; Becton Countries not listed: +49 6184 90 6940 or Dickinson and Company +33 2 2803 2000 2 NCCLS – Procedures for the Han- www.thermo.com/centrifuge dling and Processing of Blood Specimens; Approved Standard- Third Edition, H18-A3 Vol. 24 No. 38, November 2004 © 2009 Thermo Fisher Scientific Inc. All rights reserved. BD Vacu- tainer and PPT are registered trademarks of Becton, Dickinson and Company. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details.