BD Biosciences Product Catalog
BD Biosciences Product Catalog
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TABLE OF CONTENTS
GENERAL INFORMATION 9 BD PHARMINGEN RAT 181 (TRANSDUCTION LABORATORIES) BD Biosciences Company Profile 10 BRAND PRODUCTS 115 Antibodies to Rat Leukocytes and Related Cells 182
Facilities 11 Antibodies to Rat Major Histocompatibility 188 Systems Products 117 Complex (MHC) Antigens How To Order 12 Cytometric Bead Array (CBA) 118 Antibodies to Rat T-Cell Receptors (TCR) 189 Technical Services 18 DimerX MHC:Ig Recombinant Fusion Proteins 120 Product Warranties 21 ELISPOT Instrumentation and Reagents 122 NON-HUMAN PRIMATE 191 Trademarks, Patents, and Abbreviations 24 IMag Magnetic Particles 126 Antibodies for Non-Human Primate Research 192 BD BIOSCIENCES CUSTOM Monolight 3096 Microplate Luminometer 128 Mouse Immunoglobulin Isotype Controls 195 PRODUCTS AND SERVICES 29 BD PowerBlot Western Array Screening Service 130 Rat Immunoglobulin Isotype Controls 195
BD IMMUNOCYTOMETRY RESEARCH REAGENTS 133 SYSTEMS BRAND PRODUCTS 39 OTHER NON-HUMAN SPECIES 197 IMMUNOHISTOCHEMISTRY 133 Systems Products 41 Antibodies to Dog Leukocytes 198
Instruments and Data Management 57 Antibodies to Guinea Pig Leukocytes 198 Kits 134 Sample Preparation and Instrument Setup 79 Antibodies to Pig Leukocytes 198 Detection Systems and Substrates 135 Service and Customer Education 83 Antibodies to Rabbit Leukocytes 199 Multiple Tissue Arrays (MTA) 136
Buffers and Fixatives 137 CLINICAL & RESEARCH REAGENTS 93 HUMAN 201 Antibodies for Human IHC 138
Tumor Markers/Cell Biology 140 System Reagents 94 Antibodies to Human Leukocytes 202 Antibodies for Mouse IHC 142 MultiTEST Four-Color Reagents 94 Antibodies to Cytokine and Chemokine Receptors 217 Antibodies for Rat IHC 144 TriTEST Three-Color & Oncomark Reagents 95 Antibodies to Human MHC Antigens 218 Antibodies for Immunocytochemistry 145 Antibodies to Human Erythrocytes 218 Isotype Controls and Second Step Reagents 146 (Blood Group Antigens) Simultest Two-Color Reagents 96 Antibodies to Human T-Cell Receptor 219 QuanitBRITE Reagents 97 MOUSE 149 Cell Proliferation Markers 220 Dendritic Cell Reagents 97 Antibodies to Human Immunoglobulins 221 FastImmune Activation System Reagents 97 Antibodies to Mouse Leukocytes and Related Cells 150 Miscellaneous Antibodies 222 FastImmune Cytokine System Reagents 98 Antibodies to Mouse Major Histocompatibility 168 Mouse Immunoglobulin Isotype Controls 224 Custom Reagent Solutions 99 Complex (MHC) Antigens Rat Immunoglobulin Isotype Controls 225 Single-color Reagents 100 Antibodies to Mouse T-Cell Receptors (TCR) 171 Miscellaneous Reagents 225 Second-step Reagents 105 Mouse Immunoglobulin Isotype Controls 174 Isotype Controls 106 Rat Immunoglobulin Isotype Controls 176 CONTINUED ON PAGE 3 DNA Reagents 106 Hamster Immunoglobulin Isotype Controls 178
Instrument Setup and Maintenance 107 Mouse Cell Separation - IMag Particles 179 and Separation Sets Sample Preparation 107
Quality Control 108
Medimachine Instrument and Products 109 BD BIOSCIENCES PRODUCT CATALOG
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TABLE OF CONTENTS
ADHESION 227 CYTOKINES & CHEMOKINES 257 MOLECULAR BIOLOGY 349
Antibodies to Mouse Adhesion Molecules - 228 Antibodies to Mouse Cytokines, Chemokines, 258 Baculovirus Reagents 350 Selectins and their Receptors Antibodies to Recombinant Fusion Proteins 353 Antibodies to Mouse Adhesion Molecules - 229 Mouse Cytokines and Chemokines 262 Immunoglobulin Superfamily Mammalian Expression Vectors 353 Antibodies to Human Cytokines, Chemokines, 263 Antibodies to Mouse Adhesion Molecules - 230 and their Receptors EBV in-situ Probe 354 Integrins Human Cytokines and Chemokines 270 BD RiboQuant Multi-Probe Ribonuclease 355 Antibodies to Mouse Adhesion Molecules - 233 Protection Assay (RPA) System Others Antibodies to Rat Cytokines, Chemokines, 271 and their Receptors BD RiboQuant Multi-Probe Template Sets 356 Antibodies to Rat Adhesion Molecules - 234 RNA Isolation 362 Selectins Rat Cytokines and Chemokines 272 Antibodies to Viral Cytokines 272 Telomere Length and Telomeric Repeat 362 Antibodies to Rat Adhesion Molecules - 234 Amplification Protocol (TRAP) Assay Kits Immunoglobulin Superfamily Antibodies to Porcine Cytokines 272 Luciferase Assay Reagents and Luminometer 362 Antibodies to Rat Adhesion Molecules - 235 Porcine Cytokines 272 Integrins Reagents for DNA Analysis 363 Antibodies to Rabbit Cytokines, Chemokines, 273 Antibodies to Rat Adhesion Molecules - 236 and their Receptors Others HUMAN HORMONE & 365 Rabbit Cytokines 273 INFECTIOUS AGENTS Antibodies to Human Adhesion Molecules - 236 Selectins Reagents for Mouse and Human Cytokine 273 Immunocytochemistry Antibodies to Human Adhesion Molecules - 237 FLOW SUPPORT & 369 Reagents for Intracellular Flow Cytometry 274 Immunoglobulin Superfamily CELL SEPARATION Antibodies to Human Adhesion Molecules - 238 Isotype Controls 275 Integrins Positive Control Cells 276 Flow Cytometry Support Reagents 370 Antibodies to Human Adhesion Molecules - 241 BD OptEIA ELISA Sets 277 Others SPHERO Fluorescent Particles 371 BD OptEIA ELISA Kits 279 Antibodies to Human Adhesion Molecules - 242 SPHERO Rainbow Calibration Particles 371 Matrix Metalloproteinases and their Inhibitors Mouse ELISPOT Reagent 280 Positive Control Cells 372
Human ELISPOT Reagent 280 IMag Magnetic Particles and Separation Sets 373 DBOCECSPOUTCATALOG PRODUCT BD BIOSCIENCES IG/2ND STEP 245 Cytometric Bead Array (CBA) Products 280
DimerX Products 280 Immunoglobulin Isotyping Kits 246
Monoclonal Antibodies Specific for 247 Mouse Immunoglobulin Isotypes CELL BIOLOGY/CELL SIGNALING 283
Monoclonal Antibodies Specific for 249 Mouse Immunoglobulin Allotypes Antibodies 284
Monoclonal Antibodies Specific for 250 Antibody Sampler Kit 339 Rat Immunoglobulin Isotypes & Allotypes Apoptosis Related Kit, Sets, and Reagents 340 Monoclonal Antibodies Specific for 251 Caspase Enzymes, Substrates, and Inhibitors 341 Hamster Immunoglobulin Isotypes Positive Control Lysates 342 Monoclonal Antibodies Specific for 252 Human Immunoglobulin Isotypes Positive Control Lysate Kits 344
Monoclonal Antibodies Specific for 253 Recombinant Proteins and Peptides 345 Baboon Immunoglobulin Isotypes Accessory Reagents 346 Rabbit and Other Immunoglobulin Reagents 253
Mouse Anti-Trinitrophenol (TNP) 253 Monoclonal Antibodies
Polyclonal Second-step Reagents 254
Avidin/streptavidin Conjugated 255 Second-step Reagents
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BD BIOSCIENCES COMPANY PROFILE
FACILITIES
HOW TO ORDER
TECHNICAL SERVICES
PRODUCT WARRANTIES
TRADEMARKS, PATENTS, AND ABBREVIATIONS
CUSTOM PRODUCTS AND SERVICES
General Information GENERAL INFORMATION i General Information General Information
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BD BIOSCIENCES
BD Biosciences Company Profile
BD Biosciences is a provider of products and services for researchers and technicians around the world, offering integrated solutions supporting the life sciences and accelerating the pace of discovery and diagnosis.
BD Biosciences is an operating division of BD (Becton, Dickinson and Company), built on BD’s 100-year old foun- dation of quality, reliability and commitment to customers BD Biosciences and business partners around the world. BD Biosciences is Clontech comprised of the Immunocytometry Systems, Pharmingen Discovery Labware (Transduction Laboratories), Discovery Labware, and Clontech Immunocytometry Systems businesses, the companies you have known and trusted for Pharmingen years. From genes to proteins to cells, BD Biosciences provides the most comprehensive portfolio of reagents, systems, and technical expertise to support the life sciences arena.
BD Biosciences offers integrated, high-value applications for drug discovery and development, immune function monitoring, and functional genomics. Customers include academic and government institutions doing basic research in life sciences; biotech and pharma companies engaged in drug discovery and development; and hospitals, reference labs and blood banks performing patient testing and monitoring for quality control. BD Biosciences' research and clinical diagnostic systems are the standard for studying and monitoring patients with HIV. GENERAL INFORMATION
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BD BIOSCIENCES
TO REQUEST ANOTHER Our Facilities BD BIOSCIENCES COMPANY CATALOG BD Biosciences Immunocytometry Systems, located in San The BD Biosciences Catalog you have Jose, California for over 20 years, develops, manufactures, and markets clinical and research products and instrumentation received contains products from worldwide. Immunocytometry Systems is a leader in flow Immunocytometry Systems, cytometry, providing reagents, data analysis, instruments, Pharmingen and Transduction and systems for research and clinical diagnostic applications. Laboratories. BD Biosciences Pharmingen has two central locations in San Diego, CA and Lexington, Kentucky. Pharmingen corporate To request a Discovery Labware headquarters is in San Diego located on a six acre campus in the Torrey Pines area, approximately one mile north of the catalog, please call: 1.800.343.2035 University of California at San Diego and in close proximity (in the US) or visit to the Scripps Research Institute and Salk Institute. http://www.bdbiosciences.com Pharmingen offers the widest portfolio of reagents for /labware/Order.html for international immunology, molecular biology and cell biology applica- ordering information. tions. Pharmingen, the largest biotechnology employer in San Diego, currently employs over 300 people with a variety of experience in product development, manufacturing, busi- To request a Clontech catalog, ness development, marketing, and management. please call 1.800.662.2566 or The 20,000 square foot Lexington, Kentucky facility houses
1.650.424.8222 (in the US) or visit GENERAL INFORMATION the signal transduction research and development team. http://www.clontech.com Signal transduction encompasses a multidisciplinary area of /clontech/Contact.shtml research that is focused on the understanding of inter-and for international ordering information. intracellular communication. The scientific expertise and technological capabilities at this site drive an excellent and rapid product development program that allows the researcher immediate access to the best monoclonal anti- body reagents.
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ORDERING INFORMATION
Telephone Ordering 1. CUSTOMER SERVICE
To obtain more information on BD Biosciences complete Immunocytometry Systems line of reagents please contact the Customer Service depart- 1.1. Flow cytometry instruments and ment at 1.877.232.8995 (toll free), contact your local reagents from Immunocytometry BD Biosciences representative, or visit our web site at Systems and imaging systems from Biometric Imaging www.bdbiosciences.com. Outside the US, please contact the 1.1.1. Reagent orders international office in your region. A current listing of our 1.1.2. Instrument orders international offices can be found on the back cover of this 1.1.3. Literature and workshop catalog. Information 1.1.4. Technical support BD Biosciences Telephone Order System - 1.877.232.8995 Pharmingen (Transduction Laboratories) The sidebar at right outlines the choices presented to 1.2. Research reagents from Pharmingen and Transduction Laboratories customers calling the above toll-free number. For example, 2.1.1 will direct a customer to Instrument Support for Discovery Labware 1.3. BD Biocoat™ and BD Falcon™ Immunocytometry Systems products. brand cell culture vessels, reagents and pipettes
2. TECHNICAL SUPPORT
Immunocytometry Systems 2.1. Flow cytometry instruments and reagents from Immunocytometry Systems and imaging systems from Biometric Imaging 2.1.1. Instrument support 2.1.2. Computer and network support 2.1.3. Reagent, software, application support 2.1.4. Service contract and spare parts 2.1.5. Customer education and training – FACS Academy Training 2.1.6. Documents FAX 2.1.7. Technical support GENERAL INFORMATION Pharmingen (Transduction Laboratories) 2.2. Pharmingen research reagents 2.3. Transduction Laboratories cell signaling reagents Discovery Labware 2.4. BD Biocoat and BD Falcon brand cell culture vessels, reagents and pipettes
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ORDERING INFORMATION
MAIL ORDERS Mail Ordering
BD Biosciences For the most efficient order processing, please include the Pharmingen (Transduction Laboratories) following information on your purchase order or corre- spondence: Attn: Customer Service Department 10975 Torreyana Road 1. Purchase order number San Diego, California 92121-1106 2. Shipping address FAX: 1.800.325.9637 (toll free) 3. Billing address or 858.812.8889 4. Catalog number and description Immunocytometry Systems 5. Quantity and size required Attn: Sales Administration Department 2350 Qume Drive 6. Name and phone number of purchasing agent San Jose, California 95131-1807 7. Name and phone number of end user Attn: Sales Administration Department 8. Quotation number (if applicable) Fax: 408.954.2007 9. Credit card number and expiration date (if applicable)
Written confirmations are not required for orders placed by telephone. If one must be sent, mark boldly "Confirming Order - Do Not Ship." BD Biosciences cannot issue credit or be held responsible for duplicate shipments resulting from improperly GENERAL INFORMATION marked confirming orders. Please note that BD Biosciences offers credit card purchases on any of our products; have your VISA or MasterCard account number available when placing an order.
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ORDERING INFORMATION
Conditions Of Sale PLEASE SEND ALL PAYMENTS TO OUR LOCKBOX AT: Terms are Net 30 days. Prices listed in the US version of this cat- alog are listed in US dollars and are valid only in the US. Pharmingen (Transduction Laboratories) BD Biosciences reserves the right to revise pricing without notice. P.O. Box 30350, File 55494 Los Angeles, CA 90074-5494 Return & Replacement Policy Or
Upon receipt of any product from BD Biosciences, the buyer Immunocytometry Systems should inspect the packaging and products. Damages can File 5446 occur during shipping and handling. The Customer Service Los Angeles, CA 90074 department should be notified immediately by phone, fol- lowed by written documentation of any claims for shortages, defects, or damages. To confirm receipt of noticeably damaged goods; the buyer should immediately notify the delivery service office and save the packing/packaging materials for inspection by the delivery service. BD Biosciences is not liable for any loss or damage to the product beyond our rea- sonable control during the course of shipment. This includes accidents, theft, vandalism, labor difficulties or disputes, weather extremes, other acts of nature, or any other circumstances causing a delay in delivery. A return authorization is required for all returns. Please contact the Customer Service department toll free within the US: 1.877.232.8995 (1.1. for Immunocytometry Systems or 2.2. for Pharmingen [Transduction Laboratories]) to receive authorization for returns or any additional information or clarification of our policy.
Restocking fee GENERAL INFORMATION In the case of a purchasing error or change in order subsequent to shipping the product, BD Biosciences will charge a 25% restocking fee or minimum of $25, whichever is greater. All shipping costs are to be paid by the buyer. If BD Biosciences makes a shipping error, the product will be replaced free of charge.
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ORDERING INFORMATION
Shipping
Pharmingen (Transduction Laboratories)
All shipments are F.O.B. San Diego, CA. All orders processed by 2:00 pm (PST) Monday through Thursday, and orders for non-frozen items processed by 11:00 am (PST) Friday, will be shipped the same day for next day delivery within the US. Friday orders for items shipped frozen will be shipped the following Monday for next day delivery within the US.
Freight and handling charges need to be prepaid and are added to the invoice.
Immunocytometry Systems
All shipments are F.O.B. San Jose, CA. All reagent orders received and processed by 2:00 pm (PST) for delivery within the US will be shipped the same day for overnight delivery.
In an effort to be friendlier to the environment, BD Biosciences is seeking ways to reduce solid waste. Within the US, most
of our products are shipped in environmentally compatible, GENERAL INFORMATION recyclable, padded envelopes without ice packs. We have performed shipping experiments that show that these reagents are stable when shipped at ambient temperature. All other products shipped with dry ice or ice packs are shipped according to their appropriate shipping conditions. We ask that, upon arrival, all products be placed under the conditions indicated on the technical data sheet.
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WARRANTIES AND PRODUCT USE
BD Biosciences Warranties and Product Use Limitations
Pharmingen (Transduction Laboratories)
Pharmingen (Transduction Laboratories) warrants that standard catalog items conform to descriptions contained in the product specific literature and technical data sheets. Pharmingen (Transduction Laboratories) is not liable for any claims or applications not listed in the company litera- ture. BD Biosciences Pharmingen (Transduction Laboratories) products are:
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAG- NOSTIC OR THERAPEUTIC PROCEDURES.
The presence of sodium azide and/or endotoxin in antibody preparations can affect the results in in vitro and in vivo functional studies. Therefore BD Biosciences Pharmingen offers a wide variety of its products in a no azide/low endo- toxin (NA/LE™) format which is specially formulated for these studies. For more information, please contact our Technical Services department.
Immunocytometry Systems
Immunocytometry Systems sells products for research and clinical use. Research products are labeled for Research Use Only (RUO) and are not for use in diagnostic or therapeutic procedures. Although not required, these products are manufactured in accordance with Good Manufacturing Practices. Products for clinical use are labeled as Analyte Specific Reagents (ASR) or for In Vitro Diagnostic Use (IVD). GENERAL INFORMATION For Analyte Specific Reagents (ASR), analytical and per- formance characteristics are not established. BD Biosciences is not liable for the misuse of any product.
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ORDERING INFORMATION
Special Orders and Custom Services
BD Biosciences strives to provide a wide variety of formats and sizes of our broad selection of reagents. If you cannot locate a reagent that meets your needs, we will try to accommodate you through our Special Order and Custom Services program. Each Special Order and Custom Service is custom tailored to meet your requirements and handled on a case-by-case basis. Please call BD Biosciences Customer Service at 877.232.8995 to inquire about availability and pricing of the following Special Orders and Custom Services:
• Bulk packaging
• No azide/low endotoxin (NA/LE™) formats
• Specific antibody concentration
• Custom conjugation of an antibody with a fluorochrome or enzyme
• Conjugation of customer’s antibody with a fluorochrome or enzyme
• Baculovirus services GENERAL INFORMATION
• Cloning, generation and amplification of recombinant baculoviruses
• Protein expression and purification
• BD RiboQuant™ Custom Services - production of custom Multi-Probe Template Sets
Detailed Custom Product information can be found in the Custom Products & Services section of this catalog.
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TECHNICAL SERVICE
BD Biosciences Technical Service Pharmingen (Transduction Laboratories)
The Technical Service department is dedicated to providing our customers with the most current and accurate information related to our full line of products and their applications. Each of our Technical Service representatives welcomes customer input, comments and suggestions that will assist BD Biosciences in providing the highest quality products and services to the scientific community.
Please call us at 1.877.232.8995 and follow the prompts in the phone tree for Pharmingen (2.2.) and Transduction Laboratories (2.3.) technical support or dial 1.800.TALKTEC (825.5832) directly in the US. Fax your inquiries to 1.800.325.9637 (toll free in the US) or 858.812.8888, or send your email to [email protected]. The Technical Service department hours are 6:30 am to 5:30 pm (PST), Monday through Friday. Our international customers may contact the inter- national office in their respective region (see complete list on the back cover of this catalog).
Immunocytometry Systems
The Immunocytometry Systems Customer Support Center is dedicated to providing customers with the most current and accurate information related to our full line of products and their applications. Each of our Customer Support representatives welcomes customer input, comments and suggestions that will assist BD Biosciences in providing the highest quality products and services to the scientific community. Please call us at 1.877.232.8995 (2.1.7) or fax your inquiry to GENERAL INFORMATION 408.954.2007.
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TECHNICAL SERVICE
BD Biosciences Technical Service Immunocytometry Systems (continued)
The Customer Support Center’s hours for application sup- port is 9:00 am (EST) to 8:00 pm (EST), Monday through Friday. Any call received during off-hours will be picked up by our answering service and logged-in for a prompt call back the next morning, at 9:00 AM (EST).
Technical Service Instrument Support
BD Biosciences regular business hours for instrument support is 8:00 am (EST) to 8:00 pm (EST)—live call processing. Instrument Support for after hours coverage is 8:00 pm (EST) to 12:00 am (EST), Monday through Friday. Your call will be picked up by our answering service, who will contact the Customer Support engineer on duty between these hours, with an expected call back of 20 minutes. Instrument support after hours is also available from 12:00 am (EST) to 8:00 am (EST), Monday through Sunday. If your call is received in this time frame, it will be picked up by our answering service and forwarded to our Mansfield, MA GENERAL INFORMATION office for prompt call back at 8:00 am (EST) the next morning. Holiday/weekend hours (Saturday and Sunday) are available at 8:00 am (EST) to 8:00 pm (EST). Your call will be picked up by our answering service, who will contact the Customer Support engineer on duty for that weekend/holiday, with an expected call back of 20 minutes. Our international customers may contact the international office in their respective region (see complete list on the back cover of this catalog), or contact us directly by phone at 1.877.232.8995 or by fax at 408.954.2007.
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TECHNICAL SERVICE
Technical Application Specialist Program
The BD Biosciences Technical Application specialist (TAS) Program, introduced late in 1998, is an important new initiative of our Marketing program. The TAS program is designed to be a multipurpose vehicle for personalized information exchange between customers, colleagues, collaborators, and our company. The mission of the program is to transform scientific discovery into quality products. In tandem with our Sales personnel, the TAS Program is a field-based appli- cation support program that provides enhanced technical sales support for Immunocytometry Systems, and Pharmingen (Transduction Laboratories) products. Activities of the TAS Program include product licensing, facilitating customer collaborations, on-site applications and instrument support, seminars, tutorials, workshops, literature shows, demon- strations, troubleshooting, and technical support of BD Biosciences products. To contact your regional Technical Applications specialist, please call 1.877.232.8995 (2.2) in the US.
Technology Transfer
Have you developed reagents that can be of interest to the research community? If so, are you overwhelmed by numerous requests for these reagents? BD Biosciences can help by commercializing your reagents. To date, BD Biosciences has established over 700 mutually beneficial license arrange- ments with over 300 institutions worldwide. In addition, BD Biosciences will also produce and provide you with samples of your own reagents so you can conserve valuable resources.
GENERAL INFORMATION If you have questions about the technology transfer process, please feel free to contact us. We look forward to having the opportunity to work with you. For more information, please call our Business Development staff at 858.812.8800.
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PRODUCT WARRANTIES
Reagent Warranties
BD Biosciences warrants that the products sold are war- ranted only to conform to the quantity and contents stated on the label at the time of delivery to the customer. There are no warranties, expressed or implied, that extend beyond the description on the label of the product. BD Biosciences sole liability is limited to either replacement of the products or refund of the purchase price. BD Biosciences is not liable for property damage, personal injury, or economic loss caused by the product.
Instrument Warranties
The instrument warranty applies only to the original pur- chaser of the instrument, option, accessory or upgrade and only to products purchased and installed by an authorized BD Biosciences service representative. Options purchased at the same time as the instrument are included in the instrument warranty and will be covered by subsequently purchased serv- ice contracts. Options purchased after the initial instrument purchase will assume the warranty or service contract of the GENERAL INFORMATION original instrument. If the purchase occurs during the last three months of the system warranty or service contract, or later, it will be considered a stand alone purchase and will receive a three month warranty from the date of installation or a four month warranty from date of shipment, whichever occurs first.
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PRODUCT WARRANTIES
Instrument Warranties (continued)
If the original instrument is no longer under warranty or service contract, the option will have a three month warranty from the date of installation or a four month warranty from the date of shipment, whichever occurs first. BD Biosciences will replace or repair free of charge any part, excluding con- sumable items as listed in BD Biosciences Current Parts Price Catalog, which fails within 12 months after the date of installation (as certified by the authorized BD Biosciences service representative) or 13 months after the date of ship- ment, whichever first occurs, due to defects in materials or workmanship; provided that the instrument has been oper- ated at all times in accordance with the instruction manual by, or under the direct supervision of, a certified operator who has attended the BD Biosciences training course for the instrument. One (1) preventive maintenance visit will be performed during the warranty period. Only the unexpired original warranty period on the instrument shall be in effect on any replacement parts, which parts can be new or recon- ditioned at the discretion of BD Biosciences. The warranty does not apply if the product has been damaged by accident, abuse, misuse, or misapplication, if the product has been modified without the written permission of BD Biosciences, or if the BD Biosciences serial number has been removed or defaced. Products not manufactured by BD Biosciences (pass through) will carry the warranty direct from the specified manufacturer or supplier. GENERAL INFORMATION
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PRODUCT WARRANTIES
Software Warranties
Software is covered by a one-year policy under which revi- sions released during the twelve-month period from original installation will be provided at no charge. The foregoing is exclusive and in lieu of all other warranties either express or implied, including warranty of merchantability and fitness for a particular purpose. Purchaser agrees that BD Biosciences is not responsible for any indirect, consequential, or busi- ness damages that can be suffered. GENERAL INFORMATION
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TRADEMARKS AND PATENTS
FACS Loader, FACSConnect, HLA-B27, IndexSort, BD BIOSCIENCES LoaderManager, Pulse Processing Plus, SortWatch, TurboSort Option, TurboSort Plus, and WorklistManager are recognized TRADEMARKS AND PATENTS product names of Becton, Dickinson and Company. Attractors™ Ficoll-Paque is a registered trademark of Amersham Pharmacia ™ Biotech, Inc. AutoCOMP BaculoGold Macintosh is a registered trademark of Apple Computer, Inc. BD Pharmingen™ CEQer, 4T8, IMAGN, and STELLer are registered trademarks of CaliBRITE™ Biometric Imaging, Inc. CaliBRITE 3™ Enterprise, PowerTrack, and LaserPure are trademarks and Innova CellFIT™ and Enterprise are registered trademarks of Coherent, Inc. CellFIX™ Filcon, Medimachine, and Medicon are trademarks of CellQuest™ Consul TS, Inc. CellWASH™ Timbuktu is a trademark of Farallon Computers. CEQer® CloneCyt™ Texas Red is a trademark of Molecular Probes, Inc. CloneCyt™ Plus CD-Chex and Retic-Chex are registered trademarks of CyChrome Streck Laboratories, Inc. CycleTEST™ Plus FlowJo is a trademark of Tree Star, Inc. FACS™ ™ ModFit LT and QuantiCALC are trademarks of Verity Software FACSCalibur House, Inc. FACScan™ ™ Please Note: The trademarks of other companies referenced in this FACSComp catalog: ABTS is a registered trademark of Boehringer Mannheim FACSCount™ GmbH. Sphero is a trademark of Spherotech, Inc. Ni-NTA Agarose FACService is a metal chelating agent developed by Hoffman-LaRoche and FACSFlow™ manufactured exclusively by QIAGEN. APO-DIRECT and APO-BRDU are trademarks of Phoenix Flow Systems. FACS™ Lysing Solution FACSNet™ Phycoerythrin (PE) conjugates and Allophycocyanin (APC): US ™ Patent No. 4,520,110; European Patent No. 76,695; Canadian FACSort Patent No. 1,179,942 FACS™ Permeabilizing Solution ™ FACS Lysing Solution/Simultest Lysing Solution: US Patent Nos. FACStar 4,654,312; 4,902,613; and 5,098,849 FACStarPLUS™ FACStation™ CD34 (Anti–HPCA-1, Anti–HPCA-2): US Patent Nos. 4,714,680; ™ 4,965,204; and 5,035,994 FACS Vantage FACSVantage™ SE GENERAL INFORMATION CD62P: US Patent No. 4,783,330 Falcon™ Method for displaying multiparameter data sets to aid in the analy- FastImmune™ sis of data characteristics (PAINT-A-GATE) US Patent No. FcBlock™ 4,845,653 FluoroBlok™ IMAGN® 2000 IndexSort™ Leu™
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TRADEMARKS AND PATENTS
Peridinin Chlorophyll Protein (PerCP): US Patent No. 4,876,190 BD BIOSCIENCES TRADEMARKS AND PATENTS Cy5.5: US Patent Nos. 5,268,486; 5,486,616; 5,569,587; 5,569,766; and 5,627,027
™ LeucoCOUNT Retic-COUNT: US Patent Nos. 4,883,867 and 4,957,870
™ LeucoGATE CD3/CD16+CD56: US Patent No. 4,895,796 LYSYS™ II Simultest IMK-Lymphocyte: US Patent Nos. 4,987,086 and ™ MacroSORT Plus 5,064,616 Matrigel™ Simultest IMK Plus: US Patent Nos. 4,987,086 and 5,064,616 Medimachine™ Multi-Clone™ Particle sorting using a moving capture tube: US Patent No. 5,030,002 MultiSET™ MultiTEST™ CD44: US Patent No. 5,108,904 ™ NA/LE Bone marrow cell lineage analysis with CD45: US Patent No. Oncomark™ 5,137,809 ™ OptEIA Method for data transformation: US Patent No. 5,224,058 (New Optilux™ View feature of PAINT-A-GATEPRO) PAINT-A-GATE™ FACSCount: US Patent No. 5,627,037 PAINT-A-GATE PLUS™ PAINT-A-GATE PRO™ Attractors: US Patent Nos. 5,627,040; 5,739,000; and 5,795,727 PharRed™ Lyse/no-wash method: US Patent No. 5,776,709 ProCOUNT™ Immune status monitoring using activation antigen: US Patent Nos. QuantiBRITE™ 5,445,939; 5,656,446; and 5,843,689; European Patent No. Retic-COUNT™ 319,543; Canadian Patent No. 1,296,622; Australia Patent No. GENERAL INFORMATION RiboQuant™ 615,880; Japan Patent No. 2,769,156 SimulSET™ R-Phycoerythrin (R-PE) and Allophycocyanin: US Patent Nos. Simultest™ 4,520,110; and 4,524,104; European Patent No. 0,076,695; Canadian Patent No. 1,179, 942 Simultest™ Control Simultest™ IMK Plus PCR is a process patented by Hoffman-LaRoche. US Patent No. 5,424,400. Simultest™ IMK- Lymphocyle SortWatch™ Sennes Drug Innovations, Inc. (Houston, TX) has granted BD SYBR® Pharmingen exclusive rights to commercialize the p21 monoclonal and polyclonal antibody technology for non-commercial research ® STELLer purposes. Transduction Laboratories TeloQuant™ technology is licensed from Geron Corporation US TriTEST™ Patent Nos. 5,629,154;5,489,508; 5,639,613; and 5,648,215. TruCOUNT™ Controls TruCOUNT™ Tubes A patent is pending for 12G5 (University of Pennsylvania). TruRed™ TurboSort Option™ TurboSort Plus™ VACUTAINER® ViaSante™
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ABBREVIATIONS
Abbreviations
The following are common abbreviations that you will find used throughout the BD Biosciences Catalog
Allophycocyanin APC Applications APPS Analyte Specific Reagent ASR Analytical and performance characteristics are not established. (Co)-stimulation Cost 2nd Step 2step Baboon Bab Baculovirus expression BV Bioassay Bio Blocking Block Cell separation Sep Controls Ctrl Cytotoxicity Cyt Cynomolgus Cyno Enzyme-linked immunosorbent assay ELISA Enzyme-linked immunospot ELISPOT Electron microscopy EM Electrophoresis EP Enzyme assay EA Fluoresecin isothiocyanate FITC Flow cytometry FCM Flow cytometry/immunoassay FCM/IA Fluorescence activated cell sorting FACS Fluorescence microscopy FM Fluorescence quantitation FQ Functional assay FA
GENERAL INFORMATION Gel shift GF Guinea pig G Pig Hamster Hm Immunocytochemistry (cytospins) ICC Immunofluorescence IF
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ABBREVIATIONS
Abbreviations (continued)
Immunohistochemistry IHC Immunohistochemistry - zinc-fixed IHC(Zn) Immunohistochemistry - frozen IHC(Fr) Immunohistochemistry - IHC(F)* Formalin (antigen retrieval required) Immunohistochemistry - paraffin IHC(F) Immunoprecipitation IP Immunoprecipitation/Western blot IP/WB In situ hybridization ISH For In Vitro Diagnostic Use IVD In vitro kinase assay IVK Intracellular staining (flow cytometry) IC/FCM Isotype control Ictrl Human Hu Kilodalton Kda Mouse Ms Neutralization Neu Phycoerythrin PE PE-Texas Red PE-TR Peridinin chlorophyll protein PerCP
Peridinin chlorophyll protein-cyanin 5.5 PerCP-Cy5.5 GENERAL INFORMATION Northern blot NB Reactivity REACT Radioimmunoassay RIA Ribonuclease Protection Assay RPA Rhesus Rhe For Research Use Only RUO Not for use in diagnostic or therapeutic procedures For Research Use Only RUO(GMP) (Good Manufacturing Practices) Not for use in diagnostic or therapeutic procedures. Although not required, these products are manufactured in accordance with Good Manufacturing Practices. Western blot WB
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BD BIOSCIENCES COMPANY PROFILE
FACILITIES
HOW TO ORDER
TECHNICAL SERVICES
PRODUCT WARRANTIES
TRADEMARKS, PATENTS, AND ABBREVIATIONS
CUSTOM PRODUCTS AND SERVICES
Custom Products & Services CUSTOM PRODUCTS & SERVICES i General Information Custom Products & Services
30
CUSTOM PRODUCTS & SERVICES
Custom Products and Services
BD Biosciences Custom Products and Services Group, is a synthesis of all of the custom programs from our various business units. We’re now linked together to create a pow- erful and unique resource for you, our customer. Now, you can explore the product and concept opportunities afforded by the largest array of life science research technologies under one roof. We offer a broad and diverse range of tech- nologies – be it at a genetic, protein, cellular or system level to enable you to get exactly what you need, and to get the job done right.
Assay design and development
BD Biosciences is recognized by top pharmaceutical, biotech and companies as a key provider of value added assays to assist in drug development and evaluation efforts. We offer a wide range of assay design and development services aimed at assessing and monitoring the efficacy, tolerability and safety parameters of candidate therapeutics and vaccines in cancer, infectious disease, autoimmunity, and transplantation. CUSTOM PRODUCTS & SERVICES
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CUSTOM PRODUCTS & SERVICES
BD BIOSCIENCES CUSTOM PRODUCTS Products to meet your needs AND SERVICES GROUP With our team of customer focused experts, we will work For more information please contact closely with you to create powerful solutions to meet your unique challenges and constraints. By drawing from the 1.888.401.4BDB in the USA or email strength and breadth of technologies that are unique to BD us at [email protected]. For Biosciences, we are confident that we can exceed your international inquiries please contact expectations, whether it involves genes, proteins, or cells your local representative. and their environments. Add to this, our fully equipped state of the art facilities, access to a staff of over 200 Ph.D.’s and the inside product knowledge that only a man- ufacturer can offer, you can see why we’re confident that we can help you get where you need to go.
Service on demand
To truly provide a unique range of custom solutions, we provide specialized lab service to support early phase clinical trial efforts. In addition to running your custom assay we can also run a wide range of standardized assays to support your study. We offer added value services upon request, such as independent pathologist review and publication-ready CUSTOM PRODUCTS & SERVICES images.
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GENES
Expression Analysis
Product Service
ATLAS™ Gene Expression Arrays cDNA Libraries Library Generation & Screening Baculovirus services RNase Protection Assays
Molecular Biology Services
Product Service
PCR-Select™ subtraction & screening ClonCapture™ Library Screening Assay Development Cloning, identificaton and purification Plasmid production RT-PCR Analysis CUSTOM PRODUCTS & SERVICES
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PROTEINS
Immunoassays
Product Service
Assay Development Cytometric Bead Array (CBA) ELISA CUSTOM PRODUCTS & SERVICES Western Blot
Product Service
Proteomics using BD PowerBlot™ western array
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CELLS
Flow Cytometry A.
Product Service
Assay Development (PE)
Multi color reagents (available as ASR) α
Antigen Quantitation TNF- DNA/Cell Cycle Analysis High Speed Cell Sorting CD3 (Cy-Chromeª)
Cell Function Analysis
Product Service
Antigen-specific activation Cytokine flow cytometry (intracellular staining) ELISPOT Antigen presentation (DimerX MHC:Ig Dimers) Calcium mobilization Studies CUSTOM PRODUCTS & SERVICES
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CELLS
Cellular Pathology
Product Service
Immunohistochemistry and in situ TUNEL Necropsy and tissue analysis Imaging and evaluation
Bio-Assays CUSTOM PRODUCTS & SERVICES
Product Service
LPA and cytotoxicity assays (non-radioactive) Chemotaxis assay
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PLASTICWARE & INSTRUMENTATION
Specialty Coatings
Product Service
Custom coated 96 or 384 well Plates
Instrumentation
Product Service
Custom Instrumentation CUSTOM PRODUCTS & SERVICES
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CONTRACT SERVICES
Contract Services
Product Service
In vitro ADME Services Metabolic Stability Screening Permeability/Bioavailability Reaction Phenotyping Protein Binding Cytochrome P450 Inhibition Cytochrome P450 Induction Sponsor Initiated Protocols Metabolite Production CUSTOM PRODUCTS & SERVICES
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SYSTEMS PRODUCTS
INSTRUMENTS & DATA MANAGEMENT
SAMPLE PREPARATION & INSTRUMENT SETUP
SERVICE & CUSTOMER EDUCATION
CLINICAL & RESEARCH REAGENTS
Systems Products SYSTEMS PRODUCTS IS BD Immunocytometry Systems Systems Products
42
LYMPHOCYTE SUBSETTING
FACS MultiSET System IVD AUTOMATED THREE-, FOUR-COLOR LYMPHOCYTE SUBSET ENUMERATION The FACS MultiSET System is used for enumeration of lym- Reagents phocyte subsets, such as CD4+ • MultiTEST reagents T lymphocytes, in human ery- • TriTEST reagents throcyte-lysed whole blood (LWB) by flow cytometry. It is • TruCOUNT Tubes a stand-alone system that Controls requires no external hematol- • CaliBRITE bead products ogy result for absolute counts. It can provide the complete • Multi-Check control CDC-recommended immune • TruCOUNT Controls monitoring panel in just two tubes. Productivity studies show that the FACS MultiSET System’s two-tube, four-color Sample Preparation method, in comparison with the conventional six-tube, two- • FACS Lysing Solution color method, can save up to 87% of hands-on time. • BD FACS Sample Processor SP-1 Productivity can be increased further with the BD FACS Sample Processor SP-1 for automated, lyse/no-wash sample Software preparation and the FACS Loader option for automated • MultiSET software sample acquisition. • FACSComp software
The system features an easy-to-use lyse/no-wash sample • WorklistManager and LoaderManager preparation method that, when combined with MultiSET software software’s automated CD45 vs side-scatter lymphocyte gate Instruments and dynamic, population-seeking algorithms, provides automation of sample analysis. • FACSCalibur flow cytometer • FACSort flow cytometer The FACS MultiSET System has the flexibility to analyze • FACScan flow cytometer user-defined reagent combinations for research use. • FACS Loader option When samples are stained using TruCOUNT Tubes, absolute counts as well as percentages of lymphocyte sub- Product Support
sets can be enumerated. • FACS MultiSET System white paper SYSTEMS PRODUCTS • FACS MultiSET Software Overview CD-ROM movie
• FACSCalibur Operator Course
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LYMPHOCYTE SUBSETTING
DEDICATED, COMPACT CD4, CD8, AND FACSCount System IVD CD3 ABSOLUTE COUNTING The FACSCount System is a complete, dedicated system for + + + Reagents automatically counting CD4 , CD8 , and CD3 T lymphocytes. These cell counts are used to monitor the immune status of • FACSCount Reagent Kit HIV-infected patients. The compact, self-contained system, Controls incorporating instrument, reagents, and controls, eliminates the need for hematology results to obtain absolute count • FACSCount Control Kit values and simplifies the sample preparation process. The Instruments FACSCount System uses whole blood, eliminating lyse and wash steps. A unique software algorithm automatically • FACSCount instrument identifies the lymphocyte populations of interest. Easy-to- Product Support use, cost effective, and reliable in the clinical laboratory, the
• FACSCount white paper FACSCount System is designed to utilize the power and advantages of flow cytometry. SYSTEMS PRODUCTS
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LYMPHOCYTE SUBSETTING
SimulSET System IVD AUTOMATED TWO-COLOR LYMPHOCYTE SUBSET ENUMERATION The SimulSET System is designed for the automated, two- color characterization of leucocyte subsets. SimulSET pro- Reagents vides rapid, consistent, and reproducible results. For each • Simultest reagents specimen and panel of reagents, the software automatically • Simultest IMK-Lymphocyte Kit determines the FSC/SSC lymphocyte gate based on the LeucoGATE (CD45/CD14) tube, assesses purity and recov- • Simultest IMK Plus Kit ery, and sets markers based on the isotype control. The Controls FACS Loader option provides automated sample acquisi- • CaliBRITE bead products tion. • Multi-Check control
Sample Preparation
• FACS Lysing Solution
Software
• SimulSET software
• FACSComp software
• WorklistManager and LoaderManager software
Instruments
• FACSCalibur flow cytometer
• FACSort flow cytometer
• FACScan flow cytometer
• FACS Loader option SYSTEMS PRODUCTS
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NOTES SYSTEMS PRODUCTS
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TRANSFUSION MEDICINE
LeucoCOUNT System IVD RESIDUAL WHITE BLOOD CELL ENUMERATION SYSTEM The LeucoCOUNT assay is a flow cytometric method for Reagents counting residual white blood • LeucoCOUNT Kit cells (rWBCs) in platelet and • TruCOUNT Tubes red-cell packs for transfusion. Leucoreduction of blood products, Controls either by filtration or apheresis, • Multi-Check control requires a process control to • CaliBRITE bead products monitor the amount of rWBCs (leucocytes) remaining in the Software pack. The LeucoCOUNT assay • CellQuest Pro software offers an efficient alternative to tedious and operator- dependent manual methods of enumeration. • FACSComp software • WorklistManager and LoaderManager The LeucoCOUNT assay provides excellent precision, lin- software earity, and accuracy at clinically relevant levels of detection, giving the highest level of confidence in process control. Instruments High throughput is achievable with LeucoCOUNT. It is no • FACSCalibur flow cytometer longer necessary to spend hours at the microscope perform- • FACSort flow cytometer ing manual counts. LeucoCOUNT eliminates the need for a separate counting standard by combining the robust • FACScan flow cytometer absolute counting technology of TruCOUNT Tubes with • FACS Loader option LeucoCOUNT reagent optimized to stain rWBCs in leucore- Product Support duced blood products. • LeucoCOUNT white paper The FACS Loader provides walkaway data acquisition. CellQuest Pro software provides flexible data analysis. SYSTEMS PRODUCTS
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TRANSFUSION MEDICINE
PROGENITOR CELL ENUMERATION ProCOUNT System IVD SYSTEM The ProCOUNT System is an Reagents integrated, flexible, automated system for the absolute counting • ProCOUNT Progenitor Cell of CD34+ cells. It combines Enumeration Kit powerful software, unique • TruCOUNT Tubes reagents, and robust absolute counting technology to reduce Controls variability in CD34 counts. • BD Stem Cell Control Kit ProCOUNT delivers efficiency • CaliBRITE bead products in the laboratory with fast, easy sample preparation using Sample Preparation ProCOUNT reagents, and automated acquisition and analy- • FACS Lysing Solution sis using ProCOUNT software.
Software The ProCOUNT kit includes combinations of CD34, CD45,
• ProCOUNT software nucleic acid dye, isotype control reagents, and TruCOUNT
• FACSComp software Tubes. • WorklistManager and LoaderManager FACS Loader–compatible ProCOUNT software is standardized, software yet flexible, for all CD34+ cell analysis needs. ProCOUNT software allows both automated CD34 analysis or ISHAGE- Instruments type manual analysis using sequential gating. Both analysis • FACSCalibur flow cytometer types offer precision-based CD34+ absolute counts and
• automated calculations of other important CD34 parameters,
FACSort flow cytometer SYSTEMS PRODUCTS + • FACScan flow cytometer such as CD34 cells as a percentage of nucleated cells or CD45+ cells. • FACS Loader option The BD Stem Cell Control Kit is available as a separate kit Product Support for use as a process control for CD34+ stem cell • ProCOUNT white paper immunophenotyping and enumeration by flow cytometry. • ProCOUNT application note The kit consists of one vial each of CD34+ Low and CD34+
• ProCOUNT Software CD-ROM movie High controls. This material is designed to represent samples containing CD34+ cells to control antibody staining, instrument setup and performance, red blood cell lysis, and CD34+ data analysis.
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TRANSFUSION MEDICINE
HLA-B27 System IVD HLA-B27 ANTIGEN DETECTION
The HLA-B27 System is a Reagents qualitative, two-color direct • HLA-B27 Kit immunofluorescence method for rapid detection of HLA-B27 Controls
antigen expression in lysed • HLA-B27 Calibration Beads whole blood (LWB) using flow • CaliBRITE bead products cytometry. The HLA-B27 System offers a complete walkaway Sample Preparation method useful in the diagnosis • FACS Lysing Solution of individuals suspected of having rheumatic or other Software
autoimmune disorders associated with the presence of • HLA-B27 software HLA-B27 antigen. HLA-B27 testing is clinically relevant to • FACSComp software the evaluation of seronegative spondyloarthropathies. There • WorklistManager and LoaderManager is a strong association of HLA-B27 with ankylosing spondylitis software (AS) as well as with several other disorders, such as Reiter’s syndrome, psoriatic arthritis, and inflammatory bowel disease. Instruments
The HLA-B27 System offers a complete reagent and • FACSCalibur flow cytometer
calibration kit and walkaway software for instrument setup, • FACSort flow cytometer calibration, acquisition, and analysis. The automated • FACScan flow cytometer software and rapid test-result reporting provide laboratory • FACS Loader option efficiency and productivity. There is no need to perform a microcytotoxicity test to confirm results unless borderline Product Support or dim positive results are obtained. The HLA-B27 reagent • HLA-B27 white paper kit contains HLA-B27 calibration beads, Anti–HLA-B27 FITC/CD3 PE reagent, and FACS Lysing Solution. SYSTEMS PRODUCTS
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INTRACELLULAR CYTOKINE DETECTION
INTRACELLULAR CYTOKINE DETECTION FastImmune Cytokine System
Reagents The FastImmune Cytokine System combines the power of multiparameter flow cytometry with an elegant method fea- • FastImmune cytokine reagents turing whole-blood activation and detection of intracellular Controls cytokine levels. The FastImmune Cytokine System yields
• FastImmune intracellular isotype controls comprehensive, subset-specific results in just a few hours rather than days. High sensitivity and low background are • CaliBRITE bead products ensured by stringent clone selection, proprietary permeabi- Sample Preparation lization chemistry, and proven conjugation technology. Cell- surface phenotypic markers can be assessed in combination • FACS Lysing Solution with intracellular detection, defining functional properties • FACS Permeabilizing Solution of cellular subsets. For more detailed information on the • FACS Permeabilizing Solution 2 FastImmune Cytokine System, refer to our immune function (optimized for antigen-specific application notes. activation methods)
Software
• CellQuest Pro software
• FACSComp software
• PAINT-A-GATEPRO software
Instruments
• FACS brand flow cytometers SYSTEMS PRODUCTS Product Support
• FastImmune cytokine application notes
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ANTIGEN-SPECIFIC CYTOKINE DETECTION
FastImmune CD4 Cytokine System ANTIGEN-SPECIFIC CYTOKINE DETECTION
The FastImmune assay system utilizes the multiparameter power Kit Contents of flow cytometry to simultaneously measure cell activation, • FastImmune CD4 intracellular cytokine production, and cellular immunophenotyping in a detection reagent and matching single tube. isotype control reagent
Each BD FastImmune CD4 Intracellular Cytokine Detection • FastImmune CD28/CD49d Kit makes getting started easier with a simplified protocol, • FACS Lysing Solution optimized reagents, and open system for the activation antigen • FACS Permeabilizing Solution 2 of your choice. These kits can detect single-cell responses (optimized for antigen-specific from a whole blood sample in a matter of hours and are available for human IFN-γ, IL-2, and TNF-α measurements. activation methods) • FastImmune Brefelden A (BFA) Intracellular cytokine measurements in response to antigen Solution activation have been used in studies of autoimmune disease, • FastImmune EDTA Solution HIV and tumor vaccine development, allergy, and viral and infectious diseases. Controls
• CaliBRITE bead products
Software
• CellQuest Pro software
• FACSComp 4.2 software
• PAINT-A-GATEPRO software
Instruments
• FACS brand flow cytometers
Product Support
• FastImmune cytokine application notes
• Intracellular cytokine detection workshop SYSTEMS PRODUCTS
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LYMPHOCYTE FUNCTION ASSESSMENT
ASSESSING LYMPHOCYTE FUNCTION FastImmune Activation System
Reagents The FastImmune Activation System provides a rapid method (under 5 hours) for evaluating functional properties of indi- • FastImmune activation reagents vidual T-, B-, or natural killer (NK)- (CD56+) lymphocyte Controls subsets. Based on the expression of CD69, an early activa-
• FastImmune isotype controls tion antigen, this revolutionary method uses a three-color staining protocol that employs FL3 fluorescence triggering. • CaliBRITE bead products The FastImmune assay yields specific, comprehensive Sample Preparation results, surpassing the limitations of bulk proliferation assays such as tritiated thymidine incorporation. • FACS Lysing Solution FastImmune products are available in several formats to Software allow flexibility in experimental design. For more informa-
• CellQuest Pro software tion on the FastImmune Activation System, refer to our white paper. • FACSComp software
Instruments
• FACS brand flow cytometers
Product Support
• FastImmune activation white paper and Source Book procedure SYSTEMS PRODUCTS
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STANDARDIZED FLUORESCENCE QUANTITATION
QuantiBRITE System STANDARDIZED FLUORESCENCE QUANTITATION As quantitative results become increasingly important, you need a system that ensures reliable results. The Reagents QuantiBRITE System offers the first complete standardized • QuantiBRITE reagents approach to fluorescence quantitation, from acquisition to analysis. With pelletized beads, high-quality QuantiBRITE Controls PE reagents, and powerful analysis software, the • QuantiBRITE PE beads QuantiBRITE System is optimized for standardized results. • CaliBRITE bead products QuantiBRITE PE reagents are now available for use in studies of inflammatory disease and HIV. Software For a better understanding of what the QuantiBRITE • CellQuest Pro System has to offer, read our white paper. • QuantiCALC or Attractors software
• FACSComp software
Instruments
• FACS brand flow cytometers
Product Support
• QuantiBRITE white paper SYSTEMS PRODUCTS
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DNA SAMPLE PREPARATION AND ANALYSIS
SYSTEM FOR DNA SAMPLE PREPARATION DNA Analysis System AND ANALYSIS The DNA Analysis System components work together, from Reagents tissue sample to results, to eliminate many of the variables associated with ploidy and cell-cycle analysis. The • CycleTEST PLUS DNA Reagent Kit BD Medimachine provides a fast, safe, and standardized Controls process for tissue disaggregation, yielding samples that can be stained with the CycleTEST PLUS DNA Reagent Kit. To • DNA QC Particles assure that instrument setup and sample preparation are • CaliBRITE bead products appropriate, the DNA QC Particles kit provides biological Sample Preparation controls, propidium iodide stain, and beads. Once data is acquired on a FACS brand flow cytometer with • Medimachine System CellQuest Pro software, powerful ModFit LT software can Software then be used to mathematically model and report cell-cycle and ploidy status. • ModFit LT software
• CellQuest Pro software
• FACSComp software
Instruments
• FACS brand flow cytometers
Product Support
• Techniques in Cell Cycle Analysis course
• ModFit LT Workshop SYSTEMS PRODUCTS
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DENDRITIC CELL IDENTIFICATION
Dendritic Cell System DENDRITIC CELL IDENTIFICATION IN PERIPHERAL BLOOD Now CD123+ dendritic cells, CD11c+ dendritic cells, and basophils can be identified directly from peripheral blood. Reagents Based on a whole-blood flow cytometric assay, this system • Dendritic cell reagents saves time and can identify multiple dendritic cell subsets in a single tube. With the first commercially available lineage Controls cocktail, these rare cellular subsets can be identified with- • CaliBRITE bead products out altering their function or phenotype. Learn more about these dendritic cell subsets and the advantages of dendritic Sample Preparation
cell identification using flow cytometry from our applica- • FACS Lysing Solution tion note. Software
• CellQuest Pro software
• FACSComp software
• PAINT-A-GATEPRO software
Instruments
• FACS brand flow cytometers
Product Support
• Dendritic cell application note SYSTEMS PRODUCTS
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SYSTEMS PRODUCTS
INSTRUMENTS & DATA MANAGEMENT
SAMPLE PREPARATION & INSTRUMENT SETUP
SERVICE & CUSTOMER EDUCATION
CLINICAL & RESEARCH REAGENTS
Instruments & Data Management INSTRUMENTS & DATA MANAGEMENT IS BD Immunocytometry Systems Instruments & Data Management
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INSTRUMENT SYSTEMS
FACS Brand Instrument Systems FLOW CYTOMETRY INSTRUMENT SYSTEMS
Our flow cytometry systems provide the high performance Instruments and quality expected from the leader in cell analysis. Our • FACSVantage SE flow cytometer instruments are designed and optimized, from end to end, as • FACSCalibur flow cytometer complete systems that include hardware, software, and • BD LSR flow cytometer reagents. We can also work with you individually to design solutions that meet your specific needs. • FACStation Data Management System
The FACSVantage SE, BD LSR, and FACSCalibur instru- Product Support
ments provide maximum performance and reliability. • Flow cytometer operator courses
Modularity has been designed into these systems from the • Sorting application notes beginning, providing the flexibility needed today and in the future.
The FACStation Data Management System is a key compo- nent of our instrument solutions. It combines the most pow- erful computer hardware available today with unsurpassed software tools that provide the productivity and flexibility required for both clinical and research cell analysis. INSTRUMENTS & DATA MANAGEMENT INSTRUMENTS & DATA
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INSTRUMENTS & DATA MANAGEMENT
FACSVANTAGE SE SYSTEM FACSVantage SE System High-performance, high-speed cell sorter • Multicolor analysis and sorting
• FACStation Data Management System The FACSVantage SE Flow Cytometry System is the superior
• Flexible and modular for future cell sorting instrument for the research laboratory. For over 25 years, BD cell sorters have been the gold standard and upgrades are found in leading university, pharmaceutical, and • Non-rectangular sort windows biotechnology research facilities throughout the world.
To meet the demands of today’s research, we’ve improved the FACS Vantage and added options that customize it. The result is the FACSVantage SE, a flow cytometer that performs with maximum flexibility and reliability. All of this technology is accessible through a familiar user interface and efficient ergonomic design. The FACSVantage SE Flow Cytometry System is fully modular, for today’s needs and beyond. INSTRUMENTS & DATA MANAGEMENT
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INSTRUMENTS & DATA MANAGEMENT
NEW FACSDiVa Option
The FACSDiVa Option for the FACSVantage SE transforms the familiar interface of the standard FACSVantage SE into the only completely digital flow cytometer available today. Expand your research capabilities with the FACSDiVa Option that includes full interbeam compensation networks (any laser and any parameter), fluorescence detection (expansion up to 10 parameters: 2 scatter and 8 fluorescence), and 4-way sorting capability with our new QuadraSort functionality.
The FACSDiVa Option takes advantage of the familiar ergo- nomic design and easy-to-use interface of the FACSVantage SE. With this option, you can continue to push the envelope of flow cytometric analysis and sorting technique. INSTRUMENTS & DATA MANAGEMENT INSTRUMENTS & DATA BD Biosciences WWW.BDBIOSCIENCES.COM IS Instruments & Data Management BD Immunocytometry Systems
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INSTRUMENTS & DATA MANAGEMENT
FACSVantage SE and FACSVantage Options
Some options require a FACSVantage SE upgrade. For more ANALYTICAL OPTIONS* information contact your BD Biosciences representative. Detector Option 1
• Provides fluorescence channel for use with the second or third laser CELL SORTING OPTIONS
Detector Option 2 TurboSort Plus
• Provides additional fluorescence • Electronic and fluidic enhancements to achieve multilaser, channel for use with the second or high-speed cell sorting third laser BD FACS AccuDrop • Requires Detector Option 1 • Rapid method for determining the drop delay value which
Detector Option 3 increases accuracy and productivity for sorting
• Provides fluorescence channel for use SortWatch with the third laser • Continuous sort-stream monitoring • Includes the following: • Fault or sort-stream instability alert - Triple beam splitter - Three-laser alignment tool MacroSORT Plus - Mounting tool • Large-particle sorting
• Nozzle tips with 200-, 300-, and 400-micron diameters Detector Option 4
• Provides additional fluorescence CloneCyt Plus with IndexSort INSTRUMENTS & DATA MANAGEMENT channel for use with the third laser • Integrated cell deposition system
• Requires Detector Option 3 • Single-cell or multicell deposition into the following with high precision: Detector Option 5 - Microtiter plates • Provides fourth fluorescence channel - Microscope slides for use with the primary laser - Other collection devices
* All detector options include the hardware • Verification capabilities and appropriate optics.
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INSTRUMENTS & DATA MANAGEMENT
FACSVantage SE and FACSVantage Options (continued)
ANALYTICAL OPTIONS (cont)
Forward Scatter PMT
• Replaces the forward scatter photodiode with a photomultiplier tube for specialized applications
Third Laser Mount (laser purchased separately)
• Provides hardware and optics that allow addition of a third laser
Closed Flow System
• Closed flow channel for analysis of biohazardous samples
• Autoclavable waste container with audible overfill alarm
NEW Aerosol Management Kit*
• Aids in aerosol management for high-speed sorters
• Supplies distributed vacuum at the sort chamber when sort chamber door is closed
• Includes drip containment and HEPA filters
* Although this aids in aerosol management it does not replace good laboratory practices. All routine laboratory biological hazards protection should be followed in conjunction with the aerosol management system.
PULSE PROCESSING OPTIONS*
Pulse Processor Plus
• Provides area, width, and ratio measurements of up to three detector signals
* Two pulse processing boards are required for simultaneous pulse processing of all instrument detectors.
OMNICOMP
• Provides cross beam compensation for the FACSVantage SE operating with the FACStation INSTRUMENTS & DATA MANAGEMENT INSTRUMENTS & DATA
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INSTRUMENTS & DATA MANAGEMENT
FACSVantage SE and FACSVantage Options (continued)
ADDITIONAL INSTRUMENT OPTIONS
Refrigerator/Heater System
• Provides temperature control for both the sample and sorted fraction
Sample Splash Shields
• Provides additional droplet containment around the sample inlet port and nozzle assembly area
Chromosome Filter Set Filters necessary for acquiring chromosome data from samples stained with Hoechst 33342 and Chromomycin A3
• 475 LP
• 390 LP
Indo-1 Filter Filters necessary for acquiring calcium flux data from samples stained with Indo-1
• DM 505 SP
• DF485/22 BP
• DF405/20 BP
PerCP Filter Set
• DF 675/20 BP filter necessary for acquiring data from samples stained with PerCP
PerCP-Cy5.5 Filter Set INSTRUMENTS & DATA MANAGEMENT
• DF 695/40 BP filter necessary for acquiring data from samples stained with PerCP-Cy5.5 in combination with a single laser (488 nm)
• DF 712/21 BP filter necessary for acquiring data from samples stained with PerCP-Cy5.5 in combination with APC or APC-Cy7 and a HeNe laser (488 nm and 635 nm)
PharRed Filter Set Filters necessary for acquiring data from samples stained with PharRed
• DM 710 LP
• DF 780/60 BP
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INSTRUMENTS & DATA MANAGEMENT
FACSVantage SE and FACSVantage Options (continued)
WATER-COOLED LASER OPTIONS
Enterprise IIC
• Provides simultaneous lines at 488 nm (maximum output of 180 mW) and 351 to 364 nm (maximum output of 50 mW)
• Includes UV/488 beam splitter, custom base plate, remote module, and all necessary cables and hoses
LaserPure 5I Water-Air Heat Exchanger
• Water-to-air heat exchanger for the Enterprise IIC laser system
Innova 302C Krypton-Ion Laser
• Provides lines at the following wavelengths: - Multiline violet, 600 mW at 406.7 to 415.4 nm - Multiline UV, 500 mW at 337.5 to 356.4 nm - 300 mW at 413.1 nm - 200 mW at 406.7 nm
• Includes PowerTrack and remote control
LaserPure 40 Water-Air Heat Exchanger
• Water-to-air heat exchanger for one laser of the Innova 90 series (all) and 300 series (all), or 2 of the Innova 70 series (all) or Enterprise lasers
Innova 304C Argon-Ion Laser
• Provides lines at the following wavelengths: - Multiline visible, 4 W at 457.9 nm to 514.5 nm - 200 mW at 528.7 nm - 1.7 W at 514.5 nm - 1.3 W at 488 nm - 250 mW at 457.9 nm - Multiline UV, 200 mW at 351 to 364 nm
• Includes PowerTrack and remote control INSTRUMENTS & DATA MANAGEMENT INSTRUMENTS & DATA
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INSTRUMENTS & DATA MANAGEMENT
FACSVantage SE and FACSVantage Options (continued)
WATER-COOLED LASER OPTIONS (cont)
Innova 305C Argon-Ion Laser
• Provides lines at the following wavelengths: - Multiline visible, 5 W at 457.9 nm to 514.5 nm - 350 mW at 528.7 nm - 2 W at 514.5 nm - 1.5 W at 488 nm - 350 mW at 457.9 nm - Multiline UV, 400 mW at 351 to 364 nm
• Includes PowerTrack and remote control
LaserPure 20 Water-Air Heat Exchanger
• Water-to-air heat exchanger for one laser of the Innova 90 series (all), 70 series (all), Spectrum laser, and Enterprise laser or any 2 Enterprise lasers
Innova 306C Argon-Ion Laser
• Provides lines at the following wavelengths: - Multiline visible, 6 W at 457.9 nm to 514.5 nm - 420 mW at 528.7 nm - 2.4 W at 514.5 nm INSTRUMENTS & DATA MANAGEMENT - 1.8 W at 488 nm - 420 mW at 457.9 nm - Multiline UV, 500 mW at 351 to 364 nm
• Includes PowerTrack and remote control
Innova 70C-4 Argon-Ion Laser
• Provides 1.3 W at 488 nm and 1.7 W at 514.5 nm
• Includes remote control
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FACSVantage SE and FACSVantage Options (continued)
WATER-COOLED LASER OPTIONS (cont)
Innova 70C-Spectrum Argon-Ion Laser (mixed argon-krypton gas laser)
• Provides lines at 647, 568.3, 530.9, 520.8, 514.5, 488, and 457.9 nm, and 50 mW UV
• Includes remote control
Innova 90C-4 Argon-Ion Laser
• Provides lines at the following wavelengths: - Multiline visible, 6 W at 457.9 nm to 514.5 nm - 300 mW at 528.7 nm - 1.7 W at 514.5 nm - 1.3 W at 488 nm - 300 mW at 457.9 nm - Multiline UV, 200 mW at 351 to 364 nm
• Includes PowerTrack and remote control
Innova 90C-5 Argon-Ion Laser
• Provides lines at the following wavelengths: - Multiline visible, 5 W at 457.9 nm to 514.5 nm - 350 mW at 528.7 nm - 2 W at 514.5 nm - 1.5 W at 488 nm - 350 mW at 457.9 nm - Multiline UV, 300 mW at 351 to 364 nm
• Includes PowerTrack and remote control
CR599 Dye Laser
• Provides tunable beam output between 580 and 620 nm using Rhodamine 6G as medium
• Used as a second or third laser
• Requires a 4- or 5-watt argon-ion pump laser INSTRUMENTS & DATA MANAGEMENT INSTRUMENTS & DATA
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FACSVantage SE and FACSVantage Options (continued)
AIR-COOLED LASER OPTIONS
Spectra Physics 177G Laser
• Provides 100 mW at 488 nm
Spectra Physics Helium-Neon Laser
• Provides 35 mW at 633 nm
Omnichrome Helium-Cadmium Laser
• Provides 35 mW at 325 nm
• Includes remote control
Power Meter for Helium-Cadmium Laser
LaserPure 60 Water-Air Heat Exchanger
• Water-to-air heat exchanger for two lasers of the Innova 300 series
FACS VANTAGE TO FACSVANTAGE SE UPGRADE
• Provides the base functionality of the FACSVantage SE by adding all new, high-performance electronics, fluidics, software, optics, and additional hardware INSTRUMENTS & DATA MANAGEMENT
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BD LSR System BD LSR SYSTEM Six-color UV-laser–based benchtop flow cytometer • Six-color fluorescence capability
Expand the range of multicolor • FACStation Data Management System
cell analysis applications you can • Flexible and modular for future run on a benchtop cytometer, upgrades with up to six fluorescence and two scatter parameters. Free up Options
your high-performance cell • 633-nm helium-neon laser sorters for high-performance cell sorting by moving your routine UV applications to the BD LSR flow cytometer. BD has combined benchtop ease-of-use with the flexibility and performance of high-end flow cytometers in an alignment- free 488/UV laser-based system or the optional 488/UV/633 three-laser system.
The BD LSR features an air-cooled 8-mW, 325-nm helium- cadmium laser in addition to a 15-mW, 488-nm argon-ion laser. An optional 633 nm helium-neon (HeNe) laser is also available. Building on the ease-of-use standard set by the FACSCalibur flow cytometry system, the BD LSR offers software instrument control, push-button fluidics, and fine- adjust sample flow-rate control so that you can concentrate on your science and not the technology. The BD LSR supports a wide range of multicolor cell analysis applications with a standard 488-nm–excited dyes and UV-excited dyes such as Hoechst 33342, DAPI, and Indo-1. With the optional HeNe laser APC can also be detected. INSTRUMENTS & DATA MANAGEMENT INSTRUMENTS & DATA
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FACSCALIBUR SYSTEM FACSCalibur System Multipurpose flow cytometer • Three- or four-color fluorescence capability The FACSCalibur Flow Cytometry System is another BD first:
• FACStation Data Management System the only four-color, dual-laser, benchtop system available today that is capable of both cell analysis and sorting. • Flexible and modular for future upgrades Designed specifically to support a wide range of applications, the FACSCalibur System is a fully integrated multiparameter system.
The FACSCalibur System combines unique dual-laser tech- nology, an automated sample loader option, and powerful software to provide the high throughput necessary to meet productivity requirements of today’s clinical laboratories. The modularity and innovative technology designed into the FACSCalibur System also offer investigators the performance and flexibility required for a variety of research applications.
The FACSCalibur’s built-in versatility meets the current demands of both clinical and research environments. Its flexibility will help you meet tomorrow’s demands. INSTRUMENTS & DATA MANAGEMENT
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FACSCalibur Options
FACSCALIBUR OPTIONS
FL4 Option
• Four-color fluorescence capability
• Red diode second laser - High sensitivity - Minimal compensation
Sorting Option
• Minimizes sort setup
• Enhances safety with aerosol-free sorting
Cell Concentrator Module
• Sorts cells directly onto filters or cell culture inserts
FACS Loader IVD
• Automates sample introduction from 12 x 75-mm tubes
NEW BD Multiwell AutoSampler
• Automates sample introduction from 96- and 384-well microtiter plates
FACS LOADER OPTIONS
Hand-Held Barcode Scanner
• Simplifies data entry
• Reduces keystrokes
Loader Racks
• Additional uniquely identified racks INSTRUMENTS & DATA MANAGEMENT INSTRUMENTS & DATA
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FACStation Data Management System
The FACStation is the most powerful data management system for cell analysis when coupled with BD flow cytometers, application software, and reagents. The power, expandability, and connectivity of the FACStation system make it an ideal choice for all research and clinical needs.
Integrated networking allows the FACStation to be connected easily with departmental/institutional servers or Laboratory Information Systems (LIS). Equipped with a large hard drive, modem, fast CD-ROM drive, and onboard backup device, the FACStation is a complete data management workstation. The FACStation provides access to the extensive array of flow cytometry software available from BD. INSTRUMENTS & DATA MANAGEMENT
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FACStation Data Management System (continued)
FACSTATION FOR FACSVANTAGE SE
This specially configured FACStation system provides easy access to all the power and flexibility of the FACSVantage SE. The large 22" flat screen monitor, built-in Gigabit Ethernet, CD-RW drive, and a large hard drive combine to provide a powerful workstation ideally matched to your ever-changing needs. Options
• Replace monochrome laser or color inkjet printer with a high-speed color ink printer
• Replace 22" monitor with 18" LCD display
• Without printer
FACSTATION FOR BD LSR
The FACStation provided with the BD LSR allows users to take full advantage of the capabilities of the BD LSR Flow Cytometer. The FACStation includes a 17" monitor, CPU with dual processors, large hard drive, CD-RW drive, Zip Drive, USB and FireWire connectivity, and monochrome laser printer or color inkjet printer. ModFit LT and FlowJo software packages are included with the system for ultimate analysis flexibility. Options
• Replace 17" monitor with 22" color monitor
• Replace 17" monitor with 18" LCD display
• Replace monochrome laser or color inkjet printer with a high-speed color ink printer
• Without printer INSTRUMENTS & DATA MANAGEMENT INSTRUMENTS & DATA
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FACStation Data Management System (continued)
FACSTATION FOR FACSCALIBUR/FACSCAN/FACSORT
The FACStation system is designed to meet the needs of most users. With integrated networking, CD-RW drive, large hard drive capacity, and built-in Zip Drive, the FACStation is a complete workstation for all your flow cytometry computing needs. A wide range of available software and hardware options ensures that this system can be configured to meet your changing demands. Options
• Replace 17" monitor with 22" color monitor
• Replace 17" monitor with 18" LCD display
• Replace 17" monitor with 15" LCD display
• Replace monochrome laser or color inkjet printer with a high-speed color ink printer
• Without printer
FACSTATION ANALYSIS WORKSTATION
For busy laboratories additional workstations provide an ideal way of increasing throughput and productivity. The FACStation Analysis Workstation can be easily networked to other FACStation systems and the rest of your network to provide powerful data management and analysis capabilities. Options INSTRUMENTS & DATA MANAGEMENT • Replace 17" monitor with 22" color monitor
• Replace 17" monitor with 18" LCD display
• Replace 17" monitor with 15" LCD display
• Replace monochrome laser or color inkjet printer with a high-speed color ink printer
• Without printer
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Data Management Software
BD offers a range of flow cytometry software that is unsurpassed. From clinical IVD products to innovative research tools, we cover all of your data analysis needs. The following chart compares the software products available from BD to help in selecting the right package for your needs.
Software Comparison Table
Software Instrument Data Data Batch Dynamic Fluorescence FACS Loader– QC and Acquisition Analysis Analysis Population Quantitation Compatible Setup Seeking Analysis
FACSComp IVD CellQuest Pro IVD* MultiSET† IVD* ProCOUNT IVD HLA-B27 IVD SimulSET† IVD* Attractors PAINT-A-GATEPRO CBA Analysis‡ QuantiCALC ModFit LT Retic-COUNT§ IVD
* IVD for IVD-cleared assays † Provides automated absolute counts when used with TruCOUNT Tubes ‡ Requires Excel § Not IVD-cleared for Macintosh Power PCs
INSTRUMENT SETUP SOFTWARE
FACSComp Software IVD High-performance flow cytometry software with walkaway capability for instrument setup, and monitoring. Flexible options for optimizing sample setup.
• Reduces time and effort required for daily instrument setup.
• Automated instrument setup for two-, three-, and four-color flow cytometry applications
• Sample optimization INSTRUMENTS & DATA MANAGEMENT INSTRUMENTS & DATA
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Data Management Software (continued)
ACQUISITION AND ANALYSIS SOFTWARE
CellQuest Pro Software IVD for IVD-cleared assays New program functionality for flow cytometry data acquisition and analysis
• Automatic gating with the Snap-To–Region tool
• Export and share graphics in many popular formats
• Create user-defined mathematical expressions for analysis
MultiSET Software IVD for IVD-cleared assays Fully automated three- and four-color enumeration of lymphocyte subsets by flow cytometry
• Designed to support the MultiSET System
• Offers automated and manual CD45/SSC lymphocyte gating and analysis
• Supports user-defined reagents and panels in analysis
• Works with the FACS Loader for automated sample acquisition
ProCOUNT Software IVD Automated acquisition and analysis software used with ProCOUNT reagents to reduce variability of CD34 counts
• Designed to support the ProCOUNT System with TruCOUNT absolute count technology
• Allows both automated CD34 analysis and ISHAGE-type manual analysis using sequential gating
HLA-B27 Software IVD
Walkaway acquisition and analysis, and reports for HLA-B27 testing of whole blood samples INSTRUMENTS & DATA MANAGEMENT
• Designed for use in the HLA-B27 System
• Works with the FACS Loader for automated sample acquisition
• Works with FACSComp software for automated calibration
SimulSET Software IVD for IVD-cleared assays Rapid two-color enumeration of lymphocyte subsets by flow cytometry
• Designed to support the SimulSET System
• Offers automated and manual FSC/SSC lymphocyte gating and analysis
• Supports user-defined reagents and panels in analysis
• Works with the FACS Loader for automated sample acquisition
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Data Management Software (continued)
ANALYSIS SOFTWARE
Attractors Software Rapid batch analysis and classification of multicolor data
• Patented dynamic gating technology automatically tracks user-defined cell populations.
• Least populous painting algorithm allows for easy identification of small populations.
• FL2 axis automatically converts to antibodies bound per cell (ABC) when analyzing data acquired with QuantiBRITE PE reagents and QuantiBRITE PE beads for fluorescence quantitation.
PAINT-A-GATEPRO Software Interactive, multidimensional data analysis
• Patented color blending algorithm for simultaneous identification and display of multiple cell populations in all data views
• Powerful painting tools to aid with exploration of complex flow cytometric data relationships
QuantiCALC Software by Verity Software House, Inc. Standardized fluorescence quantitation analysis
• Automatic conversion of FL2 axis to antibodies bound per cell (ABC) when analyzing data acquired with QuantiBRITE PE reagents and QuantiBRITE PE beads
• Unlimited zoning capacity to investigate data ranges of interest
• Powerful database capabilities for long-term or large studies
ModFit LT Software by Verity Software House, Inc. High-performance, automated DNA results analysis
• Fast DNA analysis
• Batch processing
• Easy-to-use database output
• NEW AutoDebris, AutoAggregates, AutoLinearity, and Cycle Analyzer functions INSTRUMENTS & DATA MANAGEMENT INSTRUMENTS & DATA
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Data Management Software (continued)
ANALYSIS SOFTWARE (cont)
Retic-COUNT Software IVD* Walkaway gating and analysis, and reports for reticulocyte enumeration
• Absolute reticulocyte counts reported when RBC counts entered
• Reliable statistical precision at low level of reticulocyte counts
FlowJo Software by Tree Star, Inc. Experiment-based analysis software with powerful batching and novel display capabilities
• Publication-quality graphics
• Software compensation
• Kinetics and time-series analysis
NEW CBA Analysis Software Easy quantitation of soluable analysis using BD Cytometric Bead Array
• Automatic calculation and plotting of standard curves
• Easy-to-use program
• Complete multiplexing solution when used with FACS flow cytometric systems
* Not IVD-cleared for Macintosh Power PCs INSTRUMENTS & DATA MANAGEMENT
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INSTRUMENTS & DATA MANAGEMENT
SAMPLE PREPARATION & INSTRUMENT SETUP
SERVICE & CUSTOMER EDUCATION
CLINICAL & RESEARCH REAGENTS
Sample Preparation & Instrument Setup INSTRUMENT SETUP SAMPLE PREPARATION & IS BD Immunocytometry Systems Sample Preparation & Instrument Setup
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BD FACS Sample Processor SP-1 IVD BD FACS SAMPLE PROCESSOR SP-1 Walkaway sample-prep • Maximizes uninterrupted walkaway automation for the BD FACS MultiSET System time, freeing technologists to perform other value-added tasks The new BD FACS Sample • Reduces tech-to-tech variability, Processor SP-1 represents a major increasing consistency in assay breakthrough in automation for the flow cytometry lab. performance As a component of the • Increases your lab’s productivity FACS MultiSET System for lymphocyte subset enumeration, the SP-1 significantly reduces hands-on time and improves your lab’s productivity with walk-away automation for MultiTEST four-color and TriTEST three-color lyse/no- wash assays.
The SP-1 includes an on-board computer with LCD screen and touch pad interface. Minimal training is required, allowing your lab to experience increased productivity almost immediately.
In addition to pre-programmed protocols for MultiTEST and TriTEST assays, protocols can be customized to accom- modate applications you have developed and validated.
Patient samples, reagent vials, and a FACS Loader carousel with TruCOUNT Tubes (or standard 12 x 75-mm tubes) are placed in the SP-1. Select a protocol, then walk away. After the SP-1 completes its processing of the samples, sim- ply transfer the carousel rack from the SP-1 to your FACS Loader. Your samples are now ready to run. INSTRUMENT SETUP SAMPLE PREPARATION & SAMPLE PREPARATION
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FEATURES OF THE BD MEDIMACHINE BD Medimachine SYSTEM The BD Medimachine System is a safe, standardized sample preparation system for the automated, mechanical disaggre- • Prepares tissue quickly gation of solid animal or plant tissues for flow cytometric • Standardizes tissue disaggregation analysis, cell culture, or DNA amplification. • Is safe and easy to use
• Can be used with or without enzymes The quality of the final result in the flow cytometric analysis of any tissue depends upon the quality of the prepared suspension. Manual methods for dissociating tissue can be time consuming and all have limitations. Some tissues disaggregate more readily than others and some, such as abnormal tissue, can include very delicate cells. In addition, cell viability, cell yield, and reproducibility can be low. The BD Medimachine System facilitates standardizing the prepa- ration of all tissue types.
The Medimachine System consists of three components: the Medimachine, Medicons, and Filcons. Various pore sizes of Medicons and Filcons allow the operator to optimize experimental conditions to suit the tissue under study, ensuring clean, viable preparations of single cells, cell clusters, or cell nuclei. The procedure is fast and simple.
1. Load the Medicon with tissue and buffer. Insert into the Medimachine.
2. Turn on the Medimachine for the specified time and INSTRUMENT SETUP SAMPLE PREPARATION & remove the Medicon.
3. Filter through a Filcon into the desired vessel.
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INSTRUMENTS & DATA MANAGEMENT
SAMPLE PREPARATION & INSTRUMENT SETUP
SERVICE & CUSTOMER EDUCATION
CLINICAL & RESEARCH REAGENTS
Service & Customer Education SERVICE & CUSTOMER EDUCATIONSERVICE IS BD Immunocytometry Systems Service & Customer Education
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FACService CUSTOMER-FOCUSED SOLUTIONS
Technical Support Services FACService SOLUTIONS
At the Technical Support Center, our highly knowledgeable Telephone Technical Support staff is ready to assist you with all facets of flow cytometry • Instrument support available seven and cell analysis. Our instruments and applications specialists days a week provide technical support on a diverse array of topics, • Software, network, reagent, and including sample preparation, sorting, data analysis, and computer networking. Backed by our national network of applications support available five representatives, support centers, training centers, and part days a week banks, FACService is ready to meet your needs. From your On-Site Applications and Training initial call through completion of service, our fully automated system provides a complete history of your service activity Support that lets you get service and parts promptly and efficiently. • Customized or advanced flow cytometry training and support To help you find answers to your questions in your lab, our applications specialists provide customized or advanced Technical Newsletter flow cytometry training and support at your facility. If you FACService TECHNOTES need to make arrangements, please contact us. • Quarterly clinical and research updates We keep you informed with regular issues of our newsletter. FACService TECHNOTES provides clinical and research Fax-On-Demand updates, as well as quality technical information distributed • Current methods, procedures, and quarterly. data sheets SERVICE & CUSTOMER EDUCATION
BD Biosciences WWW.BDBIOSCIENCES.COM SERVICE & CUSTOMER EDUCATION 85 IS encesences ii oscosc ii 1.877.232.8995 BD BBD B (continued) . Technical Support Services Support Services Technical data current methods, procedures, and Call us to order be day or night. These documents will sheets at any time, minutes, with high-resolution quality. faxed to you within to go online with BD Biosciences is the first in the industry can perform We remote access system. the FACSConnect of your cytometer real-time assessment and troubleshooting exactly what you and data by monitoring on our terminal see on yours. cytometry research and For the latest developments in flow Our website has a clinical application, visit our website. methods, proce- wealth of technical information including and clinical updates, dures, data sheets, application notes, as well as links to other important sites. call Support Services, For any Technical (prompt 2) BD Immunocytometry Systems Immunocytometry BD (cont) 1.877.232.8995 FACService SOLUTIONS CUSTOMER-FOCUSED On-site training tools Training Operator training courses Specialty workshops Response time of 24 to 48 hours Five- or seven- day coverage Unlimited service visits and important links Latest developments in flow cytometry and clinical applications of information at your disposal Wealth Connection via your FACStation modem and an analog phone line Enables real-time assessment of your assessment Enables real-time staff data by our support • • • • • Services Education and Training • • Service Contracts • • BD Biosciences Website www.bdbiosciences.com • FACSConnect Remote Access FACSConnect • FACSERVICE SOLUTIONS SOLUTIONS FACSERVICE Service & Customer Education & Customer Service US ORDERS: SERVICE & CUSTOMER EDUCATION 86 IS Service Contracts CUSTOMER-FOCUSEDSOLUTIONS FACService (prompt 2) sales orservicerepresentativecall maintenance inspections.ContactyourlocalBDBiosciences option youchooseshouldincludesemi-annualpreventative programs. Pleasenotefordual-laserFACSCaliburs the We alsoofferannualandsemi-annualpreventivemaintenance revisions. service contractsincludetrainingdiscountsandsoftware 48 hourstoarequestforemergencyon-siteservice.Most instrument uptime.We guaranteearesponsetimeof24or Our timelymaintenanceandsupportprogramsmaximize service visitsorprovidedonatimeandmaterialbasis. options includefive-orseven-daycoveragewithunlimited the needsofbothclinicalandresearchusers.Ourservice We offerawidevarietyofserviceoptionsaimedatmeeting BD ImmunocytometrySystems BD B i for moreinformation. osc i ences 1.877.232.8995 Service &CustomerEducation • • • • Five- orSeven-DayCoverage SERVICE CONTRACTOPTIONS Single- andDual-LaserCoverage Five-Day EconomyCoverage • • • • • • • • WWW.BDBIOSCIENCES.COM inspection isincluded) instrument, forwhichonePM inspections (excepttheFACSCount Two preventive maintenance(PM) Training discounts Software revisions consumable items) Travel, laborandparts(excluding Two PMinspections excluding consumableitems) Travel, labor, andparts(laseronly for theFACSCount instrument) FACSConnect service(notavailable One PMinspection Training discounts Software revisions consumable items) Travel, labor, andparts(excluding for theFACSCount instrument) FACSConnect service(notavailable SERVICE & CUSTOMER EDUCATION 87 IS ences i osc i BD B 48 hrs 48 hrs 24 hrs 24 hrs 48 hrs 48 hrs 48 hrs 48 hrs 48 hrs 24 hrs 24 hrs 24 hrs 24 hrs (5-Day) Laser Laser Response Time BD Immunocytometry Systems Immunocytometry BD 1.877.232.8995 FACService SOLUTIONS CUSTOMER-FOCUSED with TurboSort Option with TurboSort with TurboSort Option with TurboSort FACScan FACSort FACSCalibur SP-1 Sample Processor BD FACS IMAGN 2000 FACSCount FACStarPLUS BD LSR BD LSR 3 laser system FACSVantage SE FACSVantage Instrument Vantage FACS 5-Day 7-Day Economy Single- Dual- Guaranteed FACS Vantage FACS SE FACSVantage Service & Customer Education & Customer Service Service Plan US ORDERS: SERVICE & CUSTOMER EDUCATION 88 IS Education andTraining Services CUSTOMER-FOCUSEDSOLUTIONS FACService education. at also available complete Coordinator at courses andquestionscanbedirectedtotheEducation current withthischangingtechnology. Detailsofparticular Students leavewiththesolidfoundationittakestostay training neededtotakefulladvantageofourproducts. of nation Biosciences instructorsgivestudentstheoptimalcombi- BD from basicresearchtoclinicallaboratoryexperience, methodologies. With diversebackgroundsinflowcytometry, analysis usingstate-of-the-artflowcytometryequipmentand on-site trainingofferthehighestqualityeducationincell Our operatortrainingcourses,specialtyworkshops,and BD ImmunocytometrySystems BD B course descriptions,andaregistrationformare flow cytometrytheoryandpracticalhands-on i osc 1.877.232.8995 (prompt2) i ences our website,www.bdfacs.com/support/ . Classschedules, Service &CustomerEducation Mansfield, MA02048-1145 110 ForbesBoulevard BD ServiceCenter San Jose,CA95131-1807 2350 QumeDrive BD Biosciences educational centerlocations: courses are offered atbothofour For yourconvenience,allmajor CUSTOMER EDUCATION CENTERS WWW.BDBIOSCIENCES.COM SERVICE & CUSTOMER EDUCATION 89 IS ences i osc i BD B Two training provisions are normally offered with the pur- are normally offered with the training provisions Two is valid Each training provision chase of a flow cytometer. operator course held at BD Biosciences. for attendance at an installation. In no case This offer is valid only after instrument one year after instrument shall this offer be valid for more than the training. After the installation. Lodging is included with after it has expired, a training offer has been exercised or student. This fee does tuition fee will be charged for each not include lodging. is not trans- Operator training for a particular instrument products, nor may it be ferable to courses offered for other an additional fee. exchanged for on-site training without available if purchased Discounts on additional training are or at service contract when the flow cytometer is purchased renewal. Training Terms and Conditions Terms Training BD Immunocytometry Systems Immunocytometry BD 1.877.232.8995 FACService SOLUTIONS CUSTOMER-FOCUSED LT ModFit CellQuest Sorting Review and Sorter Options Intracellular Cytokine Detection Techniques in Cell-Cycle Analysis Techniques Analysis Chromosome FACSVantage SE–DiVa FACSVantage FACSVantage–Basics SE–Options FACSVantage FACSCalibur BD LSR FACScan/FACSort FACStation Software Overviews CD-ROM Software FACStation FACSAcademy slide sets, videos, and FACSAcademy interactive CD-ROMs Most courses offered at BD Biosciences Most courses offered as well as customized and advanced available on-site. are programs • • • • • • • WORKSHOPS SPECIALTY • • • • • OPERATOR COURSES OPERATOR • • TRAINING TOOLS ON-SITE TRAINING • Service & Customer Education & Customer Service US ORDERS: SERVICE & CUSTOMER EDUCATION 90 IS itrcieC-O)coversbasictheory; interactivetestingthroughout; 343385 343386 343384 Multilingual(English, French, German,Italian,Japanese,Spanish); Multilingual(English,French, German,Italian,Japanese,Spanish); FACSCalibur BasicswithSorting (interactiveCD-ROM) VHSformat;reviews single-,dual-,andtriple-laseralignment; (interactive CD-ROM) 343383 Immunology andFlowCytometryBasics Laser Alignment(videoandworkbook) Flowcytometrybasicsincluding:FACSCalibur andFACSVantage Introduction toFlowCytometry(35-mmslideset) rdc ecito CatalogNumber 343148 Description Shortdemonstration videosof:Attractors, FACStation Software Overviews(CD-ROM) Product Training Tools ASa/ASotOeao . ,5 nrclua yoie1495 990 1 2 IntracellularCytokine SortingReviewandSorter 2,950 2,495 4.5 4.5 FACScan/FACSsort Operator FACSCalibur Operator Course Information CUSTOMER-FOCUSEDSOLUTIONS FACService ASatg EOeao–ais45245ClQet1495 1,600 1 perday 990 2 On-SiteTraining Tuition* ModFit CellQuest Length 990 Chromosome Analysis 2,495 Note: Tuition feeslistedare subjecttochange. on-site training,feeincludescoursematerialsforthree studentsplustrainertimeandtravel.Additionalcourse materials may bepurchased at$48per set. Course/Workshop 2,495 2 *Tuition: ForcoursesheldatBDBiosciences,tuitionincludesallcoursematerials,lunches,andtransportationbetweenthehotel andBDBiosciences.For 2,495 4.5 2,495 (FACSCalibur) Techniques inCell-CycleAnalysis 4.5 4.5 FACSVantage SEOperator–DiVa FACSVantage SEOperator–Options 4.5 Tuition* FACSVantage SEOperator–Basics Length BD LSROperator Course/Workshop BD ImmunocytometrySystems BD B i osc i ences Dy)(S)(as (US$) (Days) (US$) (Days) throughout; Mac-compatibleonly covers basictheoryandoperationofthecytometer;interactivetesting Mac-compatible only FACStar PlusorFACSVantage operatortraining atBDBiosciences may bepurchased onlybyoperators whohaveattended presenter notesandhandoutmasters fluidics, optics,electronics, datadisplaysandanalysis;includes printable tutorialexercises; PC-andMac-compatible and WorklistManager software programs; contains CellQuest, CellQuestPro, FACSComp, MultiSET, SImulSET, Detection (FACSCalibur) Options (FACSVantage SE) (FACSVantage SE) LT Service &CustomerEducation WWW.BDBIOSCIENCES.COM 495 1 IS BD Immunocytometry Systems
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Clinical & Research Reagents CLINICAL & RESEARCH REAGENTS IS BD Immunocytometry Systems Clinical & Research Reagents
94 System Reagents N PRODUCT DESCRIPTION REACT APP REG SIZE CAT# E W Anti–HLA-B27 Kita Hu FCM IVD 50 tests 340183 $615
FACSCount Reagent Kitb Hu FCM IVD 50 tests 340167 $950
LeucoCOUNT Kitc Hu FCM IVD 50 tests 340523 $595
ProCOUNT Progenitor Cell Enumeration Kitd Hu FCM IVD 25 tests 340498 $390
Retic-COUNT Reagent FCM IVD 200 tests 349204 $225
aContents: Anti–HLA-B27 FITC/CD3 PE - HLA-B27 calibration beads - FACS Lysing Solution
bContents: 50 pairs of CD3/CD4 and CD3/CD8 reagents - Fixative solution (two 5-mL vials of 5% formaldehyde) - Tube caps
cContents: LeucoCOUNT reagent - TruCOUNT Tubes
dContents: Combinations of CD34, CD45, nucleic acid dye, and isotype controls - TruCOUNT Tubes
MultiTEST Four-Color Reagents
N PRODUCT DESCRIPTION REACT CLONE APP REG FORMAT SIZE CAT# E W MultiTEST CD3/CD16+CD56/CD45/CD19 Hu SK7, B73.1, NCAM16.2, FCM IVD FITC, PE, PerCP, APC 50 tests 340500 $1,095 2D1, SJ25C1
MultiTEST CD3/CD16+CD56/CD45/CD19 with TruCOUNT Tubes Hu SK7, B73.1, NCAM16.2, FCM IVD FITC, PE, PerCP, APC 50 tests 340492 $1,335 2D1, SJ25C1
MultiTEST CD3/CD8/CD45/CD4 Hu SK7, SK1, 2D1, SK3 FCM IVD FITC, PE, PerCP, APC 50 tests 340499 $1,095
MultiTEST CD3/CD8/CD45/CD4 with TruCOUNT Tubes Hu SK7, SK1, 2D1, SK3 FCM IVD FITC, PE, PerCP, APC 50 tests 340491 $1,335
MultiTEST CD4/CD38/CD3/Anti–HLA-DR Hu SK3, HB7, SK7, L243 FCM RUO (GMP) FITC, PE, PerCP, APC 50 tests 340573 $1,095
MultiTEST CD4/CD38/CD3/Anti–HLA-DR with TruCOUNT Tubes Hu SK3, HB7, SK7, L243 FCM RUO (GMP) FITC, PE, PerCP, APC 50 tests 340771 $1,335
MultiTEST CD45RA/CD45RO/CD3/CD4 Hu L48, UCHL-1, SK7, SK3 FCM RUO (GMP) FITC, PE, PerCP, APC 50 tests 340571 $1,095
MultiTEST CD45RA/CD45RO/CD3/CD4 with TruCOUNT Tubes Hu L48, UCHL-1, SK7, SK3 FCM RUO (GMP) FITC, PE, PerCP, APC 50 tests 340769 $1,335
MultiTEST CD45RA/CD45RO/CD3/CD8 Hu L48, UCHL-1, SK7, SK1 FCM RUO (GMP) FITC, PE, PerCP, APC 50 tests 340574 $1,095
MultiTEST CD45RA/CD45RO/CD3/CD8 with TruCOUNT Tubes Hu L48, UCHL-1, SK7, SK1 FCM RUO (GMP) FITC, PE, PerCP, APC 50 tests 340770 $1,335
MultiTEST CD45RA/CD62L/CD3/CD4 Hu L48, SK11, SK7, SK3 FCM RUO (GMP) FITC, PE, PerCP, APC 50 tests 340977 $1,095
MultiTEST CD45RA/CD62L/CD3/CD4 with TruCOUNT Tubes Hu L48, SK11, SK7, SK3 FCM RUO (GMP) FITC, PE, PerCP, APC 50 tests 340979 $1,335
MultiTEST CD45RA/CD62L/CD3/CD8 Hu L48, SK11, SK7, SK1 FCM RUO (GMP) FITC, PE, PerCP, APC 50 tests 340978 $1,095
MultiTEST CD45RA/CD62L/CD3/CD8 with TruCOUNT Tubes Hu L48, SK11, SK7, SK1 FCM RUO (GMP) FITC, PE, PerCP, APC 50 tests 340980 $1,335
MultiTEST CD8/CD38/CD3/Anti–HLA-DR Hu SK1, HB7, SK7, L243 FCM RUO (GMP) FITC, PE, PerCP, APC 50 tests 340572 $1,095
MultiTEST CD8/CD38/CD3/Anti–HLA-DR with TruCOUNT Tubes Hu SK1, HB7, SK7, L243 FCM RUO (GMP) FITC, PE, PerCP, APC 50 tests 340772 $1,335
MultiTEST IMK Kita Hu FCM IVD FITC, PE, PerCP, APC 50 tests 340503 $1,970
MultiTEST IMK Kit with TruCOUNT Tubesb Hu FCM IVD FITC, PE, PerCP, APC 50 tests 340504 $2,400
aContents: MultiTEST CD3/CD8/CD45/CD4 reagent - MultiTEST CD3/CD16+CD56/CD45/CD19 reagent - FACS Lysing Solution
bContents: MultiTEST CD3/CD8/CD45/CD4 reagent - MultiTEST CD3/CD16+CD56/CD45/CD19 reagent - FACS Lysing Solution CLINICAL & RESEARCH REAGENTS
BD Biosciences WWW.BDBIOSCIENCES.COM IS Clinical & Research Reagents BD Immunocytometry Systems
95 TriTEST Three-Color Reagents N PRODUCT DESCRIPTION REACT CLONE APP REG FORMAT SIZE CAT# PRICE E W TriTEST CD3/CD16+CD56/CD45 Hu SK7, B73.1, NCAM16.2, FCM IVD FITC, PE, PerCP 50 tests 340300 2D1
TriTEST CD3/CD16+CD56/CD45 with TruCOUNT Tubes Hu SK7, B73.1, NCAM16.2, FCM IVD FITC, PE, PerCP 50 tests 340403 2D1
TriTEST CD3/CD19/CD45 Hu SK7, SJ25C1, 2D1 FCM IVD FITC, PE, PerCP 50 tests 340381
TriTEST CD3/CD19/CD45 with TruCOUNT Tubes Hu SK7, SJ25C1, 2D1 FCM IVD FITC, PE, PerCP 50 tests 340405
TriTEST CD3/CD4/CD45 Hu SK7, SK3, 2D1 FCM IVD FITC, PE, PerCP 50 tests 340383
TriTEST CD3/CD4/CD45 with TruCOUNT Tubes Hu SK7, SK3, 2D1 FCM IVD FITC, PE, PerCP 50 tests 340402
TriTEST CD3/CD8/CD45 Hu SK7, SK1, 2D1 FCM IVD FITC, PE, PerCP 50 tests 340344
TriTEST CD3/CD8/CD45 with TruCOUNT Tubes Hu SK7, SK1, 2D1 FCM IVD FITC, PE, PerCP 50 tests 340406
TriTEST CD4/CD8/CD3 Hu SK3, SK1, SK7 FCM RUO (GMP) FITC, PE, PerCP 50 tests 340298
TriTEST CD4/CD8/CD3 with TruCOUNT Tubes Hu SK3, SK1, SK7 FCM IVD FITC, PE, PerCP 50 tests 340401
γ γ TriTEST 1/ 1/CD3 Isotype Control Hu X40, X40, SK7 ASR FITC, PE, PerCP 340751 γ γ TriTEST 1/ 1/CD3 Isotype Control with TruCOUNT Tubes Hu X40, X40, SK7 Ctrl RUO (GMP) FITC, PE, PerCP 50 tests 340445 γ γ TriTEST 1/ 1/CD45 Isotype Control Hu X40, X40, 2D1 Ctrl IVD FITC, PE, PerCP 50 tests 340385 γ γ TriTEST 1/ 1/CD45 Isotype Control with TruCOUNT Tubes Hu X40, X40, 2D1 Ctrl RUO (GMP) FITC, PE, PerCP 50 tests 340404
Oncomark Reagents
N PRODUCT DESCRIPTION REACT CLONE REG FORMAT CAT# PRICE E W Oncomark CD2/CD7/CD3 Hu S5.2, M-T701, SK7 RUO FITC, PE, PerCP-Cy5.5 50 tests 341130
Oncomark CD4/CD25/CD3 Hu SK3, 2A3, SK7 RUO FITC, PE, PerCP-Cy5.5 50 tests 341134
Oncomark CD8/CD56/CD3 Hu SK1, NCAM16.2, SK7 RUO FITC, PE, PerCP-Cy5.5 50 tests 341142
Oncomark CD57/CD16/CD3 Hu HNK-1, B73.1, SK7 RUO FITC, PE, PerCP-Cy5.5 50 tests 346597
Oncomark CD5/CD10/CD19 Hu L17F12, HI10a, SJ25C1 RUO FITC, PE, PerCP-Cy5.5 50 tests 341138
Oncomark FMC7/CD23/CD19 Hu FMC7, EBVCS-5, SJ25C1 RUO FITC, PE, PerCP-Cy5.5 50 tests 341148
Oncomark Anti-Kappa/Anti-Lambda/CD19 Hu TB 28-2, 1-155-2, SJ25C1 RUO FITC, PE, PerCP-Cy5.5 50 tests 341150
Oncomark Anti-Lambda/Anti-Kappa/CD20 Hu 1-155-2, TB 28-2, L27 RUO FITC, PE, PerCP-Cy5.5 50 tests 346596
Oncomark CD38/CD56/CD19 Hu HB7, NCAM16.2, SJ25C1 RUO FITC, PE, PerCP-Cy5.5 50 tests 341132 CLINICAL & RESEARCH REAGENTS
Oncomark CD103/CD22/CD20 Hu Ber-ACT 8, S-HCL-1, L27 RUO FITC, PE, PerCP-Cy5.5 50 tests 341122
Oncomark CD3/Anti-MPO/CD79a Hu UCHT1, 5B8, HM47 RUO FITC, PE, PerCP-Cy5.5 50 tests 340961
Oncomark CD45/Anti–glycophorin A/CD41a Hu 2D1, GAR-2(HIR-2), HIP8 RUO FITC, PE, PerCP-Cy5.5 50 tests 341136
Oncomark CD14/CD64 Hu MφP9, MD22 RUO FITC, PE 50 tests 341124
Oncomark CD15/CD117 Hu MMA, 104D2 RUO FITC, PE 50 tests 341126
Oncomark CD15/CD34 Hu MMA, 8G12 RUO FITC, PE 50 tests 341128
Oncomark CD7/CD33 Hu M-T701, P67.6 RUO FITC, PE 50 tests 341140
Oncomark Anti–HLA-DR/CD13 Hu L243, L138 RUO FITC, PE 50 tests 341144
Oncomark Anti–HLA-DR/CD34 Hu L243, 8G12 RUO FITC, PE 50 tests 341146
US ORDERS: 1.877.232.8995 BD Biosciences IS BD Immunocytometry Systems Clinical & Research Reagents
96 Simultest Two-Color Reagents N PRODUCT DESCRIPTION REACT CLONE APP REG FORMAT SIZE CAT# E W Simultest IMK-Lymphocyte Kita Hu FCM IVD FITC, PE 50 tests 340182 $2,400
Simultest IMK Plus Kitb Hu FCM IVD FITC, PE 50 tests 349217 $1,970
Simultest LeucoGATE (CD45/CD14) Hu 2D1, MφP9 FCM IVD FITC, PE 50 tests 340040 $425
Simultest CD3/CD4 Hu SK7, SK31 FCM IVD FITC, PE 50 tests 340043 $425
Simultest CD3/CD8 Hu SK7, SK1 FCM IVD FITC, PE 50 tests 340044 $425
Simultest CD3/CD16+CD56 Hu SK7, B73.1, MY31 FCM IVD FITC, PE 50 tests 340042 $425
Simultest CD3/CD19 Hu SK7, 4G7 FCM IVD FITC, PE 50 tests 349211 $425
Simultest CD3/Anti–HLA-DR Hu SK7, L243 FCM IVD FITC, PE 50 tests 340048 $425
Simultest CD4/CD8 Hu SK3, SK1 FCM IVD FITC, PE 50 tests 340039 $425
Simultest CD4/CD62L Hu SK3, SK11 FCM RUO (GMP) FITC, PE 50 tests 349513 $425
Simultest CD5/CD19 Hu L17F12, SJ25C1 FCM RUO (GMP) FITC, PE 50 tests 340396 $425
Simultest CD8/CD28 Hu SK1, L293 FCM RUO (GMP) FITC, PE 50 tests 340031 $420
Simultest CD8/CD38 Hu SK1, HB7 FCM RUO (GMP) FITC, PE 50 tests 349527 $425
Simultest CD8/Anti–HLA-DR Hu SK1, L243 FCM RUO (GMP) FITC, PE 50 tests 349528 $420
Simultest CD57/CD8 Hu HNK-1, SK1 FCM RUO (GMP) FITC, PE 50 tests 349510 $420
Simultest Anti-Kappa F(ab’)2/CD19 Hu Polyclonal, SJ25C1 FCM RUO (GMP) FITC, PE 50 tests 340392 $425
Simultest Anti-Lambda F(ab’)2/CD19 Hu Polyclonal, SJ25C1 FCM RUO (GMP) FITC, PE 50 tests 340393 $425
Simultest Anti-Kappa/Anti-Lambda Hu TB 28-2, 1-155-2 FCM RUO (GMP) FITC, PE 50 tests 349516 $425
γ Simultest F(ab’)2/ 1 Isotype Control Hu Polyclonal, X40 Ctrl RUO (GMP) FITC, PE 50 tests 340395 $400 γ γ Simultest 1/ 1 Isotype Control Hu X40, X40 Ctrl RUO (GMP) FITC, PE 50 tests 349526 $390 γ γ Simultest 1/ 2a Isotype Control Hu X40, X39 Ctrl IVD FITC, PE 50 tests 340041 $400 γ γ Simultest 2a/ 1 Isotype Control Hu X39, X40 Ctrl RUO (GMP) FITC, PE 50 tests 340394 $400
a γ γ Contents: FACS Lysing Solution and six Simultest reagents: - LeucoGATE (CD45/CD14) - Isotype Control ( 1/ 2a ) - CD3/CD19 - CD3/CD4 - CD3/CD8 - CD3/CD16+CD56 b γ γ Contents: FACS Lysing Solution and six Simultest reagents: - LeucoGATE (CD45/CD14) - Isotype Control ( 1/ 2a ) - CD3/CD19 - CD4/CD8 - CD3/Anti–HLA-DR - CD3/CD16+CD56 CLINICAL & RESEARCH REAGENTS
BD Biosciences WWW.BDBIOSCIENCES.COM IS Clinical & Research Reagents BD Immunocytometry Systems
97 QuantiBRITE Reagents N PRODUCT DESCRIPTION REACT CLONE ISOTYPE APP REG FORMAT SIZE CAT# E W QuantiBRITE CD38 Hu HB7 Mouse IgG1 FCM, FQ RUO (GMP) PE 50 tests 342371 $310
QuantiBRITE CD64/CD45 Hu MD22, 2D1 FCM, FQ RUO (GMP) PE, PerCP 50 tests 340768 $510
QuantiBRITE CD8/CD38/CD3 Hu SK1, HB7, SK7 FCM, FQ RUO (GMP) FITC, PE, PerCP 50 tests 341004 $710
QuantiBRITE Anti–HLA-DR/Anti-Monocyte Hu L243, MφP9 FCM, FQ RUO (GMP) PE, PerCP-Cy5.5 50 tests 340827 $510
QuantiBRITE PE Beads FCM, FQ RUO (GMP) PE 10 tubes 340495 $105
QuantiBRITE PE Custom Conjugate Hu FCM, FQ RUO (GMP) PE 50 tests 340586 $310
Dendritic Cell Reagents
N PRODUCT DESCRIPTION REACT CLONE APP REG FORMAT SIZE CAT# E W Lineage Cocktail 1 (lin 1)a Hu FCM RUO (GMP) FITC 50 tests 340546 $575
3-Color Dendritic Value Bundleb Hu FCM RUO (GMP) FITC, PE, PerCP 340566 $1,800
4-Color Dendritic Value Bundlec Hu FCM RUO (GMP) FITC, PE, PerCP, APC 340565 $1,825
aNote: The Lineage Cocktail 1 (lin 1) contains antibody clones that, in combination, stain lymphocytes, monocytes, eosinophils, and neutrophils. It includes CD3, CD14, CD16, CD19, CD20, and CD56. Peripheral blood dendritic cells and basophils can be distinguished from other leucocytes by their lack of staining with lin 1.
b α Contents: Lineage Cocktail 1 FITC (340546) - CD123 (Anti–IL-3R ) PE (340545) - CD11c PE (347637) - Anti–HLA-DR PerCP (347364) - Mouse IgG1 PE (349043) - Mouse IgG2a PE (349053) c α Contents: Lineage Cocktail 1 FITC (340546) - CD123 (Anti–IL-3R ) PE (340545) - Anti–HLA-DR PerCP (347364) - CD11c APC (340544) - Mouse IgG1 PE (349043) - Mouse IgG2a APC (340473)
FastImmune Activation System Reagents
N PRODUCT DESCRIPTION REACT CLONE APP REG FORMAT SIZE CAT# E W FastImmune CD2/CD2R Hu L303.1, L304.1 Activ Ctrl, RUO (GMP) Purified 250 µg 340366 $270 FCM
FastImmune CD69/CD3 Hu L78, SK7 FCM RUO (GMP) PE, PerCP 50 tests 340368 $430
γ FastImmune 1/CD3 Isotype Control Hu X40, SK7 Ctrl RUO (GMP) PE, PerCP 50 tests 340414 $400
FastImmune CD4/CD69/CD3 Hu SK3, L78, SK7 FCM RUO (GMP) FITC, PE, PerCP 50 tests 340365 $620
FastImmune CD8/CD69/CD3 Hu SK1, L78, SK7 FCM RUO (GMP) FITC, PE, PerCP 50 tests 340367 $620 CLINICAL & RESEARCH REAGENTS γ γ FastImmune 1/ 1/CD3 Isotype Control Hu X40, X40, SK7 Ctrl RUO (GMP) FITC, PE, PerCP 50 tests 340369 $415
FastImmune CD19/CD69/CD45 Hu SJ25C1, L78, 2D1 FCM RUO (GMP) FITC, PE, PerCP 50 tests 340418 $620
FastImmune CD56/CD69/CD45 Hu NCAM16.2, L78, 2D1 FCM RUO (GMP) FITC, PE, PerCP 50 tests 340417 $620
γ FastImmune 1/CD45 Isotype Control Hu X40, 2D1 Ctrl RUO (GMP) PE, PerCP 50 tests 340416 $400
FastImmune T-Lymphocyte Value Bundle #1a Hu FCM RUO (GMP) FITC, PE, PerCP 50 tests 340407 $1,560
FastImmune T-Lymphocyte Value Bundle #2b Hu FCM RUO (GMP) FITC, PE, PerCP 50 tests 340408 $1,440
FastImmune B-Lymphocyte Value Bundlec Hu FCM RUO (GMP) FITC, PE, PerCP 50 tests 340428 $925
FastImmune NK-Lymphocyte (CD56+) Value Bundled Hu FCM RUO (GMP) FITC, PE, PerCP 50 tests 340429 $925
a γ γ Contents: CD4/CD69/CD3 (340365) - CD8/CD69/CD3 (340367) - 1/ 1/CD3 (340369) - CD2/CD2R (340366) b γ γ Contents: CD4/CD69/CD3 (340365) - CD8/CD69/CD3 (340367) - 1/ 1/CD3 (340369) c γ Contents: CD19/CD69/CD45 (340418) - 1/CD45 (340416) d γ Contents: CD56/CD69/CD45 (340417) - 1/CD45 (340416)
US ORDERS: 1.877.232.8995 BD Biosciences IS BD Immunocytometry Systems Clinical & Research Reagents
98 FastImmune Cytokine System Reagents N PRODUCT DESCRIPTION REACT CLONE ISOTYPE APP REG FORMAT SIZE CAT# E W FastImmune CD4 Intracellular IFN-γ Detection Kita Hu FCM, IC RUO (GMP) FITC, PE, PerCP-Cy5.5 25 tests 340970 $1,400
FastImmune CD4 Intracellular IL-2 Detection Kitb Hu FCM, IC RUO (GMP) FITC, PE, PerCP-Cy5.5 25 tests 340971 $1,400
FastImmune CD4 Intracellular TNF-α Detection Kitc Hu FCM, IC RUO (GMP) FITC, PE, PerCP-Cy5.5 25 tests 340972 $1,400
FastImmune Anti–IFN-γ/CD69/CD4 Hu 25723.11, L78, SK3 FCM, IC RUO (GMP) FITC, PE, PerCP-Cy5.5 50 tests 340962 $620
FastImmune Anti–IL-2/CD69/CD4 Hu 5344.111, L78, SK3 FCM, IC RUO (GMP) FITC, PE, PerCP-Cy5.5 50 tests 340963 $620
FastImmune Anti–TNF-α/CD69/CD4 Hu 6401.1111, L78, SK3 FCM, IC RUO (GMP) FITC, PE, PerCP-Cy5.5 50 tests 340964 $620
γ γ FastImmune 2a/ 1/CD4 Isotype Control Hu X39, X40, SK3 IC Ctrl RUO (GMP) FITC, PE, PerCP-Cy5.5 50 tests 340965 $415
FastImmune Anti–IFN-γ/Anti–IL-4 Hu 25723.11, 3010.211 FCM, IC RUO (GMP) FITC, PE 50 tests 340456 $440
γ γ FastImmune 2a/ 1 Isotype Control Hu X39, X40 IC Ctrl RUO (GMP) FITC, PE 50 tests 340458 $395 (Intracellular Formulation)
FastImmune Cytokine Value Bundled FCM, IC RUO (GMP) FITC, PE, PerCP 340460 $1,140
FastImmune Anti-BrdU with DNase Hu B44 Mouse IgG1 FCM, IC, RUO (GMP) FITC 50 tests 340649 $360 Prolif
α FastImmune Anti-Human IL-1 Hu AS5 Mouse IgG1 FCM, IC RUO (GMP) FITC 50 tests 340513 $245 FCM, IC RUO (GMP) PE 50 tests 340514 $280
β FastImmune Anti-Human IL-1 Hu AS10 Mouse IgG1 FCM, IC RUO (GMP) FITC 50 tests 340515 $245 FCM, IC RUO (GMP) PE 50 tests 340516 $280
FastImmune Anti-Human IL-1ra Hu AS17 Mouse IgG1 FCM, IC RUO (GMP) PE 50 tests 340525 $280
FastImmune Anti-Human IL-2 Hu 5344.111 Mouse IgG1 FCM, IC RUO (GMP) FITC 50 tests 340448 $245 FCM, IC RUO (GMP) PE 50 tests 340450 $280
FastImmune Anti-Human IL-4 Hu 3010.211 Mouse IgG1 FCM, IC RUO (GMP) PE 50 tests 340451 $280
FastImmune Anti-Human IL-6 Hu AS12 Mouse IgG1 FCM, IC RUO (GMP) FITC 50 tests 340526 $245 FCM, IC RUO (GMP) PE 50 tests 340527 $280
FastImmune Anti-Human IL-8 Hu AS14 Mouse IgG1 FCM, IC RUO (GMP) FITC 50 tests 340509 $245 FCM, IC RUO (GMP) PE 50 tests 340510 $280
FastImmune Anti-Human IL-13 Hu JES10-5A2 Rat IgG1 FCM, IC RUO (GMP) PE 50 tests 340508 $280 γ FastImmune Anti-Human IFN- Hu 25723.11 Mouse IgG2b FCM, IC RUO (GMP) FITC 50 tests 340449 $245 FCM, IC RUO (GMP) PE 50 tests 340452 $280
α FastImmune Anti-Human TNF- Hu 6401.1111 Mouse IgG1 FCM, IC RUO (GMP) FITC 50 tests 340511 $245 FCM, IC RUO (GMP) PE 50 tests 340512 $280 FCM, IC RUO (GMP) APC 100 tests 340534 $510
γ Mouse 1 Isotype Control Hu X40 Ctrl, IC RUO (GMP) PE 50 µg 340013 $190 (Platelet/Intracellular Formulation) Ctrl
γ Mouse 2a Isotype Control (Intracellular Formulation) Hu X39 Ctrl, IC RUO (GMP) PE 25 µg 340459 $135 Ctrl
a γ γ Contents: Brefeldin A (BFA) Solution - EDTA Solution - CD28/CD49d - FACS Lysing Solution (10X) - FACS Permeabilizing Solution 2 (10X) - FastImmune 2a FITC/ 1 PE/CD4 PerCP-Cy5.5 isotype control - FastImmune Anti-Hu–IFN-γ FITC/CD69 PE/CD4 PerCP-Cy5.5
b γ γ Contents: Brefeldin A (BFA) Solution - EDTA Solution - CD28/CD49d - FACS Lysing Solution (10X) - FACS Permeabilizing Solution 2 (10X) - FastImmune 2a FITC/ 1 PE/CD4 PerCP-Cy5.5 isotype control - FastImmune Anti-Hu–IL-2 FITC/CD69 PE/CD4 PerCP-Cy5.5
c γ γ Contents: Brefeldin A (BFA) Solution - EDTA Solution - CD28/CD49d - FACS Lysing Solution (10X) - FACS Permeabilizing Solution 2 (10X) - FastImmune 2a FITC/ 1 PE/CD4 PerCP-Cy5.5 isotype control - FastImmune Anti-Hu–TNF-α FITC/CD69 PE/CD4 PerCP-Cy5.5
d γ γ γ Contents: FastImmune Anti-Hu–IFN- FITC/Anti-Hu–IL-4 PE (340456) - FastImmune 2a FITC/ 1 PE Isotype Control (340458) - CD3 PerCP (347344) CLINICAL & RESEARCH REAGENTS
BD Biosciences WWW.BDBIOSCIENCES.COM IS Clinical & Research Reagents BD Immunocytometry Systems
99 Custom Reagent Solutions N PRODUCT DESCRIPTION REACT APP REG FORMAT SIZE CAT# E W Pure Custom Conjugate Hu FCM RUO (GMP) Purified 50 tests 340350 $150
Biotin Custom Conjugate Hu FCM RUO (GMP) Biotin 50 tests 340351 $200
FITC Custom Conjugate Hu FCM RUO (GMP) FITC 50 tests 340352 $220
PE Custom Conjugate Hu FCM RUO (GMP) PE 50 tests 340353 $260
QuantiBRITE PE Custom Conjugate Hu FCM, FQ RUO (GMP) PE 50 tests 340586 $310
PerCP Custom Conjugate Hu FCM RUO (GMP) PerCP 50 tests 340354 $295
PerCP-Cy5.5 Custom Conjugate Hu FCM RUO (GMP) PerCP-Cy5.5 50 tests 340981 $310
APC Custom Conjugate Hu FCM RUO (GMP) APC 50 tests 340355 $270
Cy3 Custom Conjugate Hu FM RUO (GMP) Cy3 50 tests 340356 $220
Cy5 Custom Conjugate Hu FM RUO (GMP) Cy5 50 tests 340357 $220
PE-Cy5 Custom Conjugate Hu FCM RUO (GMP) PE-Cy5 50 tests 340358 $300
BD Immunocytometry Systems clones only. For BD PharMingen clones, contact BD PharMingen directly at 1.858.812.8844. NOTE: Some isotypes and clones are not suitable for all fluorophores. Custom conjugates are subject to a minimum order of 250 tests. Contact your local representative or call 1.800.448.2347 for more information. CLINICAL & RESEARCH REAGENTS
US ORDERS: 1.877.232.8995 BD Biosciences IS BD Immunocytometry Systems Clinical & Research Reagents
100 Single-Color Reagents N PRODUCT DESCRIPTION REACT CLONE ISOTYPE APP REG FORMAT SIZE CAT# E W
CD1a Hu SK9 Mouse IgG2b FCM RUO (GMP) Purified 100 tests 347430 $270 FCM RUO (GMP) PE 50 tests 341651 $265
CD2 Hu S5.2 Mouse IgG2a FCM RUO (GMP) Purified 100 tests 347590 $270 FCM RUO (GMP) FITC 100 tests 347593 $415 FCM RUO (GMP) PE 100 tests 347597 $475
CD3 Hu SK7 Mouse IgG1 FCM RUO (GMP) Purified 100 tests 347340 $270 FCM RUO (GMP) Biotin 100 tests 347341 $375 FCM RUO (GMP) PE 100 tests 347347 $470 FCM RUO (GMP) PerCP 100 tests 347344 $575 FCM RUO (GMP) PerCP-Cy5.5 50 tests 340949 $310 FCM RUO (GMP) APC 100 tests 340440 $510 FCM IVD FITC 50 tests 349201 $235 FCM IVD FITC 250 tests 340542 $860
CD4 Hu SK3 Mouse IgG1 FCM RUO (GMP) Purified 200 tests 346320 $525 FCM RUO (GMP) Biotin 100 tests 347321 $375 FCM RUO (GMP) PE 100 tests 347327 $470 FCM RUO (GMP) PerCP 100 tests 347324 $575 FCM RUO (GMP) PerCP-Cy5.5 50 tests 341654 $310 FCM RUO (GMP) APC 100 tests 340443 $510 FCM IVD FITC 100 tests 340133 $415
CD4 Multi-Clone Hu SK3, SK4 Mouse IgG1 FCM RUO (GMP) FITC 100 tests 347413 $420
CD4 v4 Hu L120 Mouse IgG1 FCM RUO (GMP) FITC 50 tests 340422 $240 FCM RUO (GMP) PE 50 tests 340419 $270
CD5 Hu L17F12 Mouse IgG2a FCM RUO (GMP) Purified 200 tests 346300 $525 FCM RUO (GMP) Biotin 100 tests 347301 $375 FCM RUO (GMP) FITC 100 tests 347303 $415 FCM RUO (GMP) PE 100 tests 347307 $475 FCM RUO (GMP) APC 100 tests 340583 $510
CD7 Hu 4H9 Mouse IgG2a FCM RUO (GMP) Purified 100 tests 347480 $270 FCM RUO (GMP) FITC 100 tests 347483 $415
M-T701 Mouse IgG1 FCM RUO (GMP) FITC 50 tests 340737 $235 FCM RUO (GMP) PE 50 tests 340581 $265
CD8 Hu SK1 Mouse IgG1 FCM RUO (GMP) Purified 200 tests 346310 $525 FCM RUO (GMP) Biotin 100 tests 347311 $375 FCM RUO (GMP) FITC 100 tests 347313 $415 FCM RUO (GMP) PerCP 100 tests 347314 $575 FCM RUO (GMP) APC 100 tests 340584 $510 FCM IVD PE 100 tests 340046 $475
SK2 Mouse IgG2a FCM RUO (GMP) Purified 100 tests 347350 $270 CD9 Hu M-L13 Mouse IgG1 FCM RUO (GMP) FITC 50 tests 341646 $235 FCM RUO (GMP) PE 50 tests 341647 $265 FCM RUO (GMP) PerCP-Cy5.5 50 tests 341649 $310
FCM RUO (GMP) APC 50 tests 341648 $510
CD10 Hu HI10a Mouse IgG1 FCM RUO (GMP) FITC 50 tests 340925 $235 FCM RUO (GMP) PE 50 tests 340921 $265 FCM RUO (GMP) APC 100 tests 340923 $510 CLINICAL & RESEARCH REAGENTS
BD Biosciences WWW.BDBIOSCIENCES.COM IS Clinical & Research Reagents BD Immunocytometry Systems
101 Single-Color Reagents (continued) N PRODUCT DESCRIPTION REACT CLONE ISOTYPE APP REG FORMAT SIZE CAT# E W
CD10 (cont.) W8E7 Mouse IgG2a FCM RUO (GMP) Purified 100 tests 347500 $270 FCM RUO (GMP) FITC 100 tests 347503 $415
CD11a Hu G-25.2 Mouse IgG2a FCM RUO (GMP) Purified 100 tests 347980 $270 FCM RUO (GMP) FITC 100 tests 347983 $415
CD11b Hu D12 Mouse IgG2a FCM RUO (GMP) Purified 100 tests 347550 $270 FCM RUO (GMP) PE 100 tests 347557 $475 FCM RUO (GMP) APC 100 tests 340937 $510
CD11c Hu S-HCL-3 Mouse IgG2b FCM RUO (GMP) PE 100 tests 347637 $475 FCM RUO (GMP) APC 100 tests 340544 $510
CD13 Hu L138 Mouse IgG1 FCM RUO (GMP) Purified 100 tests 347830 $270 FCM RUO (GMP) PE 100 tests 347837 $475
φ CD14 Hu M P9 Mouse IgG2b FCM RUO (GMP) Purified 100 tests 347490 $270 FCM RUO (GMP) FITC 100 tests 347493 $415 FCM RUO (GMP) PE 100 tests 347497 $475 FCM RUO (GMP) PerCP 50 tests 340585 $310 FCM RUO (GMP) APC 100 tests 340436 $510
CD15 Hu MMA Mouse IgM FCM RUO (GMP) Purified 100 tests 347420 $270 FCM RUO (GMP) FITC 100 tests 347423 $415
CD16 Hu B73.1 Mouse IgG1 FCM RUO (GMP) PE 100 tests 347617 $475 GO22 Mouse IgM FCM RUO (GMP) Purified 100 tests 347530 $270
NKP15 Mouse IgG1 FCM RUO (GMP) FITC 100 tests 347523 $415
CD18 Hu L130 Mouse IgG1 FCM RUO (GMP) Purified 100 tests 347950 $270 FCM RUO (GMP) FITC 100 tests 347953 $415
CD19 Hu 4G7 Mouse IgG1 FCM RUO (GMP) Purified 100 tests 347540 $270 FCM RUO (GMP) FITC 100 tests 347543 $415 FCM RUO (GMP) PerCP 100 tests 347544 $565 FCM IVD PE 50 tests 349209 $265
SJ25C1 Mouse IgG1 FCM RUO (GMP) FITC 50 tests 340409 $235 FCM RUO (GMP) PE 50 tests 340364 $265 FCM RUO (GMP) PerCP 50 tests 340421 $310 FCM RUO (GMP) PerCP-Cy5.5 50 tests 340951 $310
FCM RUO (GMP) APC 100 tests 340437 $510 CLINICAL & RESEARCH REAGENTS
CD20 Hu L27 Mouse IgG1 FCM RUO (GMP) Purified 100 tests 347670 $270 FCM RUO (GMP) FITC 100 tests 347673 $415 FCM RUO (GMP) PE 50 tests 346595 $265 FCM RUO (GMP) PerCP 100 tests 347674 $575 FCM RUO (GMP) PerCP-Cy5.5 50 tests 340955 $310 FCM RUO (GMP) APC 100 tests 340941 $510
CD21 Hu HB-5 Mouse IgG2a FCM RUO (GMP) Purified 100 tests 347620 $265
CD22 Hu S-HCL-1 Mouse IgG2b FCM RUO (GMP) FITC 100 tests 347573 $415 FCM RUO (GMP) PE 100 tests 347577 $475 FCM RUO (GMP) APC 100 tests 340933 $510
CD23 Hu EBVCS-5 Mouse IgG1 FCM RUO (GMP) Purified 100 tests 347790 $270 FCM RUO (GMP) PE 100 tests 347797 $475 FCM RUO (GMP) APC 100 tests 340935 $510
US ORDERS: 1.877.232.8995 BD Biosciences IS BD Immunocytometry Systems Clinical & Research Reagents
102 Single-Color Reagents (continued) N PRODUCT DESCRIPTION REACT CLONE ISOTYPE APP REG FORMAT SIZE CAT# E W
CD25 Hu 2A3 Mouse IgG1 FCM RUO (GMP) Purified 100 tests 347640 $270 FCM RUO (GMP) FITC 100 tests 347643 $415 FCM RUO (GMP) PE 100 tests 347647 $475 FCM RUO (GMP) APC 100 tests 340939 $510
CD26 Hu L272 Mouse IgG2a FCM RUO (GMP) FITC 50 tests 340426 $235 FCM RUO (GMP) PE 50 tests 340423 $265
CD27 Hu L128 Mouse IgG1 FCM RUO (GMP) FITC 50 tests 340424 $235 FCM RUO (GMP) PE 50 tests 340425 $265
a,b CD28 Hu L293 Mouse IgG1 FCM, IHC RUO (GMP) Purified 200 µg 340975 $365 FCM RUO (GMP) Purified 500 µg 348040 $570 FCM RUO (GMP) PE 100 tests 348047 $480